spirochaetales

Summary

Summary: An order of slender, flexuous, helically coiled bacteria, with one or more complete turns in the helix.

Top Publications

  1. pmc Differentiation of porcine Brachyspira species by a novel nox PCR-based restriction fragment length polymorphism analysis
    Judith Rohde
    Institut für Mikrobiologie und Tierseuchen, Tieraerztliche Hochschule Hannover, Hannover, Germany
    J Clin Microbiol 40:2598-600. 2002
  2. pmc OspC facilitates Borrelia burgdorferi invasion of Ixodes scapularis salivary glands
    Utpal Pal
    Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Clin Invest 113:220-30. 2004
  3. ncbi Recognition of two new species of intestinal spirochetes: Serpulina intermedia sp. nov. and Serpulina murdochii sp. nov
    T B Stanton
    Enteric Diseases and Food Safety Research Unit, United States Department of Agriculture, Ames, Iowa 50010, USA
    Int J Syst Bacteriol 47:1007-12. 1997
  4. pmc Comparative prevalences of Brachyspira aalborgi and Brachyspira (Serpulina) pilosicoli as etiologic agents of histologically identified intestinal spirochetosis in Australia
    A S Mikosza
    Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia
    J Clin Microbiol 39:347-50. 2001
  5. ncbi Phylogenetic foundation of spirochetes
    B J Paster
    Department of Molecular Genetics, The Forsyth Institute, Boston, Massachusetts 02115, USA
    J Mol Microbiol Biotechnol 2:341-4. 2000
  6. pmc Lipoprotein computational prediction in spirochaetal genomes
    Joao C Setubal
    Virginia Bioinformatics Institute, Virginia Tech, Bioinformatics 1, Box 0477, Blacksburg, VA 24060 0477, USA
    Microbiology 152:113-21. 2006
  7. ncbi Infection and Alzheimer's disease: the APOE epsilon4 connection and lipid metabolism
    Nadezda Urosevic
    Sir James McCusker Alzheimer Disease Research Unit, School of Psychiatry and Clinical Neurosciences, The University of Western Australia, Australia
    J Alzheimers Dis 13:421-35. 2008
  8. pmc Borrelia burgdorferi basic membrane proteins A and B participate in the genesis of Lyme arthritis
    Utpal Pal
    Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA
    J Exp Med 205:133-41. 2008
  9. ncbi Genetics of motility and chemotaxis of a fascinating group of bacteria: the spirochetes
    Nyles W Charon
    Department of Microbiology, Immunology, and Cell Biology, Health Sciences Center, West Virginia University, Box 9177, Morgantown, West Virginia 26506 9177, USA
    Annu Rev Genet 36:47-73. 2002
  10. ncbi Whole genome analyses of transporters in spirochetes: Borrelia burgdorferi and Treponema pallidum
    M H Saier
    Department of Biology, University of California at San Diego, La Jolla 92093 0116, USA
    J Mol Microbiol Biotechnol 2:393-9. 2000

Detail Information

Publications235 found, 100 shown here

  1. pmc Differentiation of porcine Brachyspira species by a novel nox PCR-based restriction fragment length polymorphism analysis
    Judith Rohde
    Institut für Mikrobiologie und Tierseuchen, Tieraerztliche Hochschule Hannover, Hannover, Germany
    J Clin Microbiol 40:2598-600. 2002
    ..The restriction patterns for Brachyspira hyodysenteriae, B. pilosicoli, B. intermedia, B. murdochii, and B. innocens were highly distinct with two restriction endonucleases only. The assay proved to be user-friendly and robust...
  2. pmc OspC facilitates Borrelia burgdorferi invasion of Ixodes scapularis salivary glands
    Utpal Pal
    Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Clin Invest 113:220-30. 2004
    ..These studies conclusively demonstrate the importance of OspC in the invasion of tick salivary glands by B. burgdorferi, a critical step in the transmission of spirochetes from the arthropod vector to the mammalian host...
  3. ncbi Recognition of two new species of intestinal spirochetes: Serpulina intermedia sp. nov. and Serpulina murdochii sp. nov
    T B Stanton
    Enteric Diseases and Food Safety Research Unit, United States Department of Agriculture, Ames, Iowa 50010, USA
    Int J Syst Bacteriol 47:1007-12. 1997
    ..pilosicoli, respectively. S. hyodysenteriae and S. intermedia DNAs gave the same fragment pattern regardless of the enzyme tested. B. aalborgi was differentiated from the Serpulina species by MboII digestion of the 16S rDNA amplicon...
  4. pmc Comparative prevalences of Brachyspira aalborgi and Brachyspira (Serpulina) pilosicoli as etiologic agents of histologically identified intestinal spirochetosis in Australia
    A S Mikosza
    Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia
    J Clin Microbiol 39:347-50. 2001
    ..This study demonstrates that B. aalborgi is much more commonly involved in histologically identified IS in Australian patients than is B. pilosicoli. This is the first report of amplification of B. pilosicoli DNA from humans with IS...
  5. ncbi Phylogenetic foundation of spirochetes
    B J Paster
    Department of Molecular Genetics, The Forsyth Institute, Boston, Massachusetts 02115, USA
    J Mol Microbiol Biotechnol 2:341-4. 2000
    ..There are now over 200 spirochetal species or phylotypes, of which more than half is presently not cultivable. It is likely that there is still a significant unrecognized spirochetal diversity that should be evaluated...
  6. pmc Lipoprotein computational prediction in spirochaetal genomes
    Joao C Setubal
    Virginia Bioinformatics Institute, Virginia Tech, Bioinformatics 1, Box 0477, Blacksburg, VA 24060 0477, USA
    Microbiology 152:113-21. 2006
    ..The hybrid approach of supplementing weight matrix scoring with rules based on knowledge of protein secretion biochemistry may be a general strategy for development of improved prediction algorithms...
  7. ncbi Infection and Alzheimer's disease: the APOE epsilon4 connection and lipid metabolism
    Nadezda Urosevic
    Sir James McCusker Alzheimer Disease Research Unit, School of Psychiatry and Clinical Neurosciences, The University of Western Australia, Australia
    J Alzheimers Dis 13:421-35. 2008
    ..This connection was discussed in the broader context of lipid metabolism and in the light of different capacity of various infectious agents, their toxic lipophilic products and host lipoprotein particles for binding to cell receptor(s)...
  8. pmc Borrelia burgdorferi basic membrane proteins A and B participate in the genesis of Lyme arthritis
    Utpal Pal
    Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA
    J Exp Med 205:133-41. 2008
    ..These data delineate a role for differentially produced B. burgdorferi antigens in spirochete colonization of mouse joints, and suggest new strategies for the treatment of Lyme arthritis...
  9. ncbi Genetics of motility and chemotaxis of a fascinating group of bacteria: the spirochetes
    Nyles W Charon
    Department of Microbiology, Immunology, and Cell Biology, Health Sciences Center, West Virginia University, Box 9177, Morgantown, West Virginia 26506 9177, USA
    Annu Rev Genet 36:47-73. 2002
    ..Motility is likely to play a major role in the disease process. This review summarizes the progress in the genetics of motility and chemotaxis of spirochetes, and points to new directions for future experimentation...
  10. ncbi Whole genome analyses of transporters in spirochetes: Borrelia burgdorferi and Treponema pallidum
    M H Saier
    Department of Biology, University of California at San Diego, La Jolla 92093 0116, USA
    J Mol Microbiol Biotechnol 2:393-9. 2000
    ..The largest Spirochete secondary carrier families consist of efflux systems. While both Bbu and Tpa exhibit an unusual degree of transporter diversity, major differences in specificity exist between these two organisms...
  11. ncbi Chronic inflammation and amyloidogenesis in Alzheimer's disease -- role of Spirochetes
    Judith Miklossy
    University of British Columbia, Kinsmen Laboratory of Neurological Research, Vancouver, BC, Canada
    J Alzheimers Dis 13:381-91. 2008
    ....
  12. ncbi Application of nox-restriction fragment length polymorphism for the differentiation of Brachyspira intestinal spirochetes isolated from pigs and poultry in Australia
    Kirsty M Townsend
    School of Veterinary Science, The University of Queensland, Brisbane, QLD 4072, Australia
    J Vet Diagn Invest 17:103-9. 2005
    ..This protocol represents a significant improvement over conventional methods currently used in veterinary diagnostic laboratories for rapid specific identification of Brachyspira spp. isolated from both pigs and poultry...
  13. ncbi PCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces
    A S Mikosza
    Division of Veterinary and Biomedical Sciences, Murdoch University, 6150, Murdoch, WA, Australia
    FEMS Microbiol Lett 197:167-70. 2001
    ..These new faecal PCR protocols should be valuable for future studies on the epidemiology of intestinal spirochaete infections in human populations, particularly as it is not currently possible to isolate B. aalborgi from faeces...
  14. ncbi The prevalence of human intestinal spirochetosis in Norway
    C F Lindboe
    Department of Pathology, Vest-Agder Central Hospital, Kristiansand, Norway
    Anim Health Res Rev 2:117-9. 2001
    ..0% in South Norway, and in both regions there was a considerable male predominance of HIS (70% and 75%, respectively). Furthermore, HIS was more often diagnosed by the pathologists in South Norway (25%) than in mid-Norway (6.5%)...
  15. ncbi Human intestinal spirochaetosis in Parma: a focus on a selected population during 2002-2005
    Simona Peruzzi
    Department of Pathology and Laboratory Medicine, Section of Microbiology, University of Parma, Parma, Italy
    Acta Biomed 78:128-32. 2007
    ..Since poor data were available on the prevalence of this infection, the aim of our study was to describe the occurrence of this infection in our area in the period 2002-2005...
  16. pmc Molecular and ultrastructural characterization of porcine hippurate-negative Brachyspira pilosicoli
    Marja Fossi
    National Veterinary and Food Research Institute, Seinajoki Unit, PB 198, 60101 Seinäjoki, Finland
    J Clin Microbiol 42:3153-8. 2004
    ..In conclusion, B. pilosicoli can be either hippurate positive or negative and, thus, the scheme for biochemical differentiation of porcine Brachyspira should be revised to include identification of hippurate-negative B. pilosicoli...
  17. ncbi Intestinal spirochaetes of the genus Brachyspira: an update on recent findings
    David J Hampson
    School of Veterinary and Biomedical Sciences, Murdoch University, Murchoch, Western Australia, 6150, Autralia
    J Vet Sci 4:1-5. 2003
  18. ncbi Infective colitis associated with human intestinal spirochetosis
    Adriana Calderaro
    Department of Pathology and Laboratory Medicine, Section of Microbiology, University of Parma, Parma, Italy
    J Gastroenterol Hepatol 22:1772-9. 2007
    ..Our study reports the detection and identification of intestinal spirochetosis in patients with colonic diseases in a tertiary-care hospital over a 12-year period, and includes a description of all cases we diagnosed...
  19. ncbi [Diagnosis of swine dysentery and spirochaetal diarrhea. III. Results of cultural and biochemical differentiation of intestinal Brachyspira species by routine culture from 1997 to 1999.]
    J Verspohl
    ,
    Dtsch Tierarztl Wochenschr 108:67-9. 2001
    ..It is recommended to use special transport media for the transport of the specimen...
  20. ncbi Comparison of methods for antimicrobial susceptibility testing and MIC values for pleuromutilin drugs for Brachyspira hyodysenteriae isolated in Germany
    Judith Rohde
    Department of Infectious Diseases, School of Veterinary Medicine Hannover, Institute for Microbiology, Bischofsholer Damm 15, 30173, Germany
    Vet Microbiol 102:25-32. 2004
    ..05), while in 2002 the average MIC significantly decreased when compared to the value in 2000 (P < 0.05). However, strains with MIC values for tiamulin as high as 8 microg/ml (broth dilution) could still be isolated...
  21. ncbi Brachyspira pilosicoli isolated from pigs in Japan
    Chollada Tasu
    Animal Health Laboratory, School of Agriculture, Ibaraki University, Japan
    J Vet Med Sci 66:875-7. 2004
    ..2 to 0.3 microm in diameter, 4 periplasmic flagella at each end were observed dominantly. The isolates were hippurate positive but indole negative. This is the first report on the isolation of B. pilosicoli from pigs in Japan...
  22. ncbi Protection of pigs from swine dysentery by vaccination with recombinant BmpB, a 29.7 kDa outer-membrane lipoprotein of Brachyspira hyodysenteriae
    Tom La
    School of Veterinary and Biomedical Sciences, Division of Veterinary and Biomedical Sciences, Murdoch University, WA 6150, Australia
    Vet Microbiol 102:97-109. 2004
    ..The incidence of disease for the three His6-BmpB vaccinated groups was significantly less (P = 0.047) than for the control groups, with a approximately 50% reduction. BmpB appears to have potential as an SD vaccine component...
  23. ncbi Gastroduodenal adenocarcinomas and rectal adenoma in a cougar (Felis concolor) infected with Helicobacter-like organisms and spirochetes
    Yoshikazu Yamazaki
    Ehime Chuo Livestock Hygiene Service Center, Masaki, Iyo, Japan
    J Vet Med Sci 64:149-53. 2002
    ..In the rectum, chronic inflammation with numerous spirochetes was also noted. These results indicated that the occurrence of the gastrointestinal tumors might be associated with the bacterial infection described above...
  24. ncbi Optic neuropathy from viruses and spirochetes
    M Tariq Bhatti
    Duke University Eye Center, 2351 Erwin Road, Durham, NC 27710, USA
    Int Ophthalmol Clin 47:37-66, ix. 2007
  25. ncbi Dietary enzyme and zinc bacitracin reduce colonisation of layer hens by the intestinal spirochaete Brachyspira intermedia
    D J Hampson
    Division of Veterinary and Biomedical Sciences, Murdoch University, WA 6150, Perth, Australia
    Vet Microbiol 86:351-60. 2002
    ..It was not established how the ZnB at 50 ppm and the dietary enzyme reduced the ability of the spirochaete to colonise, but it may have been by bringing about changes in the intestinal microflora and/or the intestinal microenvironment...
  26. pmc Development of a duplex PCR assay for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces
    Tom La
    School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia
    J Clin Microbiol 41:3372-5. 2003
    ..The feces also underwent anaerobic culture and species-specific PCRs. Fecal extraction-D-PCR detected seven additional samples containing B. hyodysenteriae and five more containing B. pilosicoli...
  27. ncbi [Genes and genetic systems in spirochetes]
    Masahito Fukunaga
    Laboratory of Molecular Microbiology, Fukuyama University, Faculty of Pharmacy and Pharmaceutical Sciences
    Nihon Rinsho 61:779-84. 2003
  28. ncbi Clinical significance of human intestinal spirochetosis--a morphologic approach
    M Korner
    Institute of Pathology, Kantonsspital Luzern, CH 6000 Luzern 16, Switzerland
    Infection 31:341-9. 2003
    ..Homosexual and HIV-positive men are more likely to be symptomatic irrespective of invasion. Rare cases of spirochetemia and multiple organ failure have been reported in critically ill patients...
  29. ncbi Rapid detection and identification of Brachyspira aalborgi from rectal biopsies and faeces of a patient
    Adriana Calderaro
    Department of Pathology and Laboratory Medicine, Section of Microbiology, University of Parma, Viale A Gramsci 14, 43100 Parma, Italy
    Res Microbiol 154:145-53. 2003
    ..2% corresponding to that of the B. aalborgi type strain. Our results encourage further investigations for the development of a suitable selective agar medium for the isolating and cultivating B. aalborgi from human specimens...
  30. ncbi Colonic spirochetosis in children and adults
    Ayman Koteish
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Clin Pathol 120:828-32. 2003
    ..While treatment information remains limited, treatment can lead to resolution of symptoms in some cases...
  31. ncbi Zinc bacitracin enhances colonization by the intestinal spirochaete Brachyspira pilosicoli in experimentally infected layer hens
    A Jamshidi
    Division of Veterinary and Biomedical Sciences, Murdoch University, WA 6150, Australia
    Avian Pathol 31:293-8. 2002
    ..001). Dietary ZnB at 50 parts/10(6) therefore predisposed to colonization by B. pilosicoli. Despite colonization, no significant production differences were found between the birds in the three groups...
  32. ncbi Influence of in-feed zinc bacitracin and tiamulin treatment on experimental avian intestinal spirochaetosis caused by Brachyspira intermedia
    D J Hampson
    Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia
    Avian Pathol 31:285-91. 2002
    ..The other 20 infected birds had a significant drop in egg production, but those receiving ZnB showed a reduced colonization by B. intermedia after 3 weeks...
  33. ncbi Intestinal spirochetosis in eight pediatric patients from Southern Sweden
    Lars Marthinsen
    Department of Pediatrics, County Hospital, Halmstad, Sweden
    APMIS 110:571-9. 2002
    ..In four there was partial improvement and in one child no change in bowel-related symptoms. We believe that intestinal spirochetes may cause colorectal disease in children. Possible pathogenic mechanisms are discussed...
  34. ncbi Intestinal spirochetosis in a 21-month-old thoroughbred colt
    Tomoyuki Shibahara
    Hokkaido Research Station, National Institute of Animal Health, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062 0045, Japan
    J Vet Med Sci 64:633-6. 2002
    ..These invasive intestinal spirochetes might be one of pathogens inducing colitis and diarrhea in horses...
  35. ncbi Darkfield microscopic spirochete count in the differentiation of endodontic and periodontal abscesses
    M Trope
    Department of Endodontology, Temple University School of Dentistry, Philadelphia, PA 19140, USA
    J Endod 18:82-6. 1992
    ..In these cases the percentage of spirochetes in the exudates of the lesions was used as an aid to diagnosis with successful treatment results...
  36. ncbi Spirochetes in otology: are we testing for the right pathogens?
    Waleed M Abuzeid
    Department of Otorhinolaryngology Head and Neck Surgery, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Otolaryngol Head Neck Surg 138:107-9. 2008
    ....
  37. ncbi [A case of intestinal spirochaetosis due to Brachyspira aalbolgi]
    Hideki Tazawa
    Department of Gastroenterological Medicine, Iwate Prefectural Hanamaki Kosei Hospital
    Nihon Shokakibyo Gakkai Zasshi 101:162-7. 2004
  38. ncbi Influence of diet on the experimental infection of pigs with Brachyspira pilosicoli
    R H Lindecrona
    Danish Veterinary Institute, DK 1790 Copenhagen V, Denmark
    Vet Rec 154:264-7. 2004
    ..05). The fermented liquid feed and the diet containing lactic acid had no significant effect on the excretion of B pilosicoli or on the numbers of pigs showing clinical signs of disease...
  39. ncbi Spirochetes--the possible etiological factor of the cheilitis granulomatosa
    H Liu
    Department of Pathology, Tongren Hospital of Capital University of Medical Sciences, Beijing 100730
    Chin Med Sci J 16:52-5. 2001
    ..After treatment by penicillin, the facial and labial swelling of the 11 cases of CG and MRS were abated. CONCLUSION: CG and MRS probably are infectious diseases caused by spirochetes...
  40. ncbi [The changes of subgingival microbials during periodontal maintenance in patients with gingivitic and wearing fixed orthodontic appliances]
    Juan Du
    Molecular Laboratory for Gene Therapy, Beijing Hospital for Stomatology, Capital University of Medical Sciences, Beijing 100050, China
    Zhonghua Kou Qiang Yi Xue Za Zhi 39:324-6. 2004
    ..To investigate the microbiological changes of subgingival microbials in patients with gingivitis and wearing fixed orthodontic appliances...
  41. ncbi The effect of fermentable carbohydrates on experimental swine dysentery and whip worm infections in pigs
    Lisbeth E Thomsen
    Danish Centre for Experimental Parasitology, Department of Veterinary Pathobiology, KVL, Dyrlaegevej 100, DK 1870 Frederiksberg C, Denmark
    Vet Microbiol 119:152-63. 2007
    ..The study showed that diets supplemented with highly fermentable carbohydrates from dried chicory roots and sweet lupins can protect pigs against developing swine dysentery, but do not have any significant influence on T. suis...
  42. ncbi Antimicrobial susceptibility testing of Brachyspira intermedia and Brachyspira pilosicoli isolates from Australian chickens
    D J Hampson
    School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia
    Avian Pathol 35:12-6. 2006
    ..pilosicoli. These data suggest that some resistance to common antimicrobials exists among intestinal spirochetes obtained from laying hens and supports the need of MIC data for clinical isolates before any treatment is considered...
  43. ncbi Typhlocolitis associated with spirochaetes in goose flocks
    C S Nemes
    Veterinary Institute of Kaposvár of the Central Veterinary Institute, Cseri u 18, H 7401, Kaposvar, Hungary
    Avian Pathol 35:4-11. 2006
    ..This is the first report on the isolation of B. alvinipulli from laying geese affected with fibrinonecrotic typhlocolitis...
  44. ncbi The Brachyspira hyodysenteriae ftnA gene: DNA vaccination and real-time PCR quantification of bacteria in a mouse model of disease
    Antony J Davis
    CSIRO Livestock Industries, AAHL, Geelong, Vic, 3220, Australia
    Curr Microbiol 50:285-91. 2005
    ..hyodysenteriae infection. Vaccine efficacy was monitored by caecal pathology and quantification of B. hyodysenteriae numbers in the caeca of infected mice by real-time PCR...
  45. ncbi Cecal spirochetosis caused by Brachyspira pilosicoli in commercial turkeys
    H L Shivaprasad
    California Animal Health and Food Safety Laboratory System, Fresno Branch University of California, Davis, 2789 S Orange Avenue, Fresno, CA 93725, USA
    Avian Dis 49:609-13. 2005
    ..To the author's knowledge, this is the first report of B. pilosicoli in the ceca of turkeys...
  46. ncbi Comparative evaluation of molecular assays for the identification of intestinal spirochaetes from diseased pigs
    A Calderaro
    Department of Pathology and Laboratory Medicine, Section of Microbiology, Faculty of Medicine and Surgery, University of Parma, Viale A Gramsci, 14 43100 Parma, Italy
    Vet Microbiol 118:91-100. 2006
    ..hyodysenteriae reserving 16S RFLP-PCR for all other pathogenic and non-pathogenic Brachyspira species. Among the species-specific PCR assays tested only that for B. pilosicoli was useful in our hands...
  47. ncbi Experimental infection of layer hens with a human isolate of Brachyspira pilosicoli
    Abdollah Jamshidi
    Division of Veterinary and Biomedical Sciences, Murdoch University, Western Australia 6150, Australia
    J Med Microbiol 52:361-4. 2003
    ..These findings confirm that B. pilosicoli strains can infect across species barriers and cause chronic mild diarrhoea in intact adult chickens...
  48. ncbi [Severe diarrhoea with invasive intestinal spirochaetosis]
    N G Koopmans
    Universiteit Maastricht, Faculteit der Geneeskunde, Maastricht
    Ned Tijdschr Geneeskd 149:2873-6. 2005
    ..The literature reports patients who improve symptomatically without specific treatment. Treatment may be given to patients with severe symptoms...
  49. ncbi Amplification and sequencing of Brachyspira spp. specific portions of nox using paraffin-embedded tissue samples from clinical colitis in Austrian pigs shows frequent solitary presence of Brachyspiramurdochii
    Herbert Weissenbock
    Institute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, University of Veterinary Medicine, Veterinarplatz 1, A 1210 Vienna, Austria
    Vet Microbiol 111:67-75. 2005
    ..These observations point at the possibility that under certain conditions even Brachyspira species of low pathogenicity can multiplicate extensively and lead to Brachyspira-associated enterocolitis...
  50. ncbi Development of a two-step nested duplex PCR assay for the rapid detection of Brachyspira pilosicoli and Brachyspira intermedia in chicken faeces
    Nyree D Phillips
    School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia
    Vet Microbiol 116:239-45. 2006
    ..The application of this new technique should improve diagnostic capacity, and facilitate further studies on AIS...
  51. ncbi Intestinal spirochetosis and chronic watery diarrhea: clinical and histological response to treatment and long-term follow up
    Maria Esteve
    Department of Gastroenterology, Mutua Terrassa Hospital, University of Barcelona, Barcelona, Catalonia, Spain
    J Gastroenterol Hepatol 21:1326-33. 2006
    ....
  52. pmc The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
    Tae Jung Kim
    School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia
    J Vet Sci 7:277-80. 2006
    ..B. hyodysenteriae and B. pilosicoli) as well as an epidemiological tool for characterizing spirochetes isolated from swine...
  53. ncbi Proposed mechanisms and preventative options of Jarisch-Herxheimer reactions
    M W Pound
    Department of Pharmacy Practice, Campbell University School of Pharmacy, Buies Creek, NC 27506, USA
    J Clin Pharm Ther 30:291-5. 2005
    ..To review the aetiologies and preventative methods associated with Jarisch-Herxheimer reactions (JHR)...
  54. ncbi The use of multilocus enzyme electrophoresis to characterise intestinal spirochaetes (Brachyspira spp.) colonising hens in commercial flocks
    Carol P Stephens
    Toowoomba Veterinary Laboratory, Biosecurity, Department of Primary Industries and Fisheries, Toowoomba, QLD 4350, Australia
    Vet Microbiol 107:149-57. 2005
    ..These results show that the epidemiology of intestinal spirochaetal infections in broiler breeder and laying hen flocks can vary considerably between farms, although the reasons for these differences were not established...
  55. pmc Penicillin-binding proteins in the pathogenic intestinal spirochete Brachyspira pilosicoli
    Rohana P Dassanayake
    Department of Veterinary and Biomedical Sciences, University of Nebraska Lincoln, Rm 147, VBS Bldg, Lincoln, Nebraska 68583 0905, USA
    Antimicrob Agents Chemother 49:1561-3. 2005
    ..Identification of PBPs of B. pilosicoli provides a basis for characterization of the genes encoding these proteins among pathogenic intestinal spirochetes of humans and animals...
  56. ncbi Risk factors associated with Brachyspira hyodysenteriae PCR-positivity in East-European pig production units
    C P Mirko
    Bilkei Consulting, Bahnhofstr 42, 8600 Dübendorf Switzerland
    Tijdschr Diergeneeskd 131:398-402. 2006
    ..There was a strong association between grower-finisher status and whether the animals were on outdoor lots, with the odds of being B. hyo PCR positive being substantially lower for pigs on outdoor lots compared with all other surfaces...
  57. ncbi Intestinal spirochetosis
    Grace Guzman
    Department of Pathology, Division of Surgical Pathology, University of Illinois Medical Center, Chicago 60612, USA
    Arch Pathol Lab Med 128:1188. 2004
  58. ncbi The organic acid profiles in the feces of pigs at Brachyspira hyodysenteriae- or B. pilosicoli-positive farms
    Shong Ji Piao
    Laboratory of Animal Science, Kyoto Prefectural University, Shimogamo, Japan
    J Vet Med Sci 69:425-8. 2007
    ..B. pilosicoli was detected in 5 samples collected from another farm. Lower concentrations of iso-butyrate and iso-valerate were likely associated with development of pathogenic spirochete infection...
  59. ncbi A cross-sectional study to investigate the occurrence and distribution of intestinal spirochaetes (Brachyspira spp.) in three flocks of laying hens
    N D Phillips
    School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch, Perth, WA 6150, Australia
    Vet Microbiol 105:189-98. 2005
    ..intermedia than the flock of intermediate age (P = 0.004), suggesting that following initial infection of individual young hens on this farm there was amplification and transmission of infection amongst members of the flock...
  60. ncbi Development of a multiplex-PCR for rapid detection of the enteric pathogens Lawsonia intracellularis, Brachyspira hyodysenteriae, and Brachyspira pilosicoli in porcine faeces
    T La
    School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA, Australia
    Lett Appl Microbiol 42:284-8. 2006
    ..To develop an assay to simultaneously detect Lawsonia intracellularis, Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig faeces...
  61. ncbi Simultaneous detection of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in swine intestinal specimens by multiplex polymerase chain reaction
    Dong Kyun Suh
    Research Institute of Health and Environment, Daegu, Korea
    J Vet Sci 6:231-7. 2005
    ..In conclusion, this multiplex PCR is a feasible alternative to standard diagnostic methods for detection of L. intracellularis, B. hyodysenteriae and Salmonella spp. from swine intestinal specimens...
  62. ncbi Detection of Lyme disease spirochete, Borrelia burgdorferi sensu lato, including three novel genotypes in ticks (Acari: Ixodidae) collected from songbirds (Passeriformes) across Canada
    John D Scott
    Lyme Disease Association of Ontario, 365 St David Street South, Fergus, Ontario, Canada N1M 2L7
    J Vector Ecol 35:124-39. 2010
    ..Because songbirds disperse millions of infected ticks across Canada, people and domestic animals contract Lyme disease outside of the known and expected range...
  63. ncbi Neotrombicula autumnalis (Acari, Trombiculidae) as a vector for Borrelia burgdorferi sensu lato?
    H Kampen
    Institute for Medical Parasitology, University of Bonn, Sigmund Freud Str, Bonn, Germany
    Exp Appl Acarol 33:93-102. 2004
    ..However, unless the transmission of borreliae to a given host is demonstrated, a final statement on the vector competence of trombiculid mites is not possible...
  64. ncbi Human thyroid autoantigens and proteins of Yersinia and Borrelia share amino acid sequence homology that includes binding motifs to HLA-DR molecules and T-cell receptor
    Salvatore Benvenga
    Sezione di Endocrinologia del Dipartimento Clinico Sperimentale di Medicina e Farmacologia, Universita di Messina, Messina, Italy
    Thyroid 16:225-36. 2006
    ..In conclusion, our in silico data do not directly demonstrate that Borrelia and Yersinia proteins trigger AITD but suggest that a restricted number of them might have the potential to, at least in persons with certain HLA-DR alleles...
  65. ncbi Diets containing inulin but not lupins help to prevent swine dysentery in experimentally challenged pigs
    C F Hansen
    Animal Research Institute, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia
    J Anim Sci 88:3327-36. 2010
    ..05). In conclusion, this study shows that diets supplemented with highly fermentable carbohydrates from inulin protected pigs against developing swine dysentery...
  66. ncbi Western gray squirrel (Rodentia: Sciuridae): a primary reservoir host of Borrelia burgdorferi in Californian oak woodlands?
    Robert S Lane
    Division of Insect Biology, Department of Environmental Science, Policy and Management, University of California, Berkeley, CA 94720, USA
    J Med Entomol 42:388-96. 2005
    ..burgdorferi s.s. in certain types of woodlands. The findings with respect to A. phagocytophilum, although of less certain significance, suggest that S. griseus could serve as a secondary host of this rickettsia...
  67. ncbi Search for eukaryotic motility proteins in spirochetes: immunological detection of a tektin-like protein in Spirochaeta halophila
    A L Barth
    Department of Botany, University of Massachusetts, Amherst 01003
    Biosystems 24:313-9. 1991
    ..Although neither centrin nor calmodulin were detected, a 30 kDa tektin-like protein apparently is present in these spirochetes...
  68. pmc Phylogenetic analysis of the spirochetes
    B J Paster
    Forsyth Dental Center, Boston, Massachusetts 02115
    J Bacteriol 173:6101-9. 1991
    ..The division of the spirochetes into six major phylogenetic clusters was defined also by sequence signature elements. These signature analyses supported the conclusion that the spirochetes represent a monophylectic bacterial phylum...
  69. ncbi Tubulinlike protein from Spirochaeta bajacaliforniensis
    D Bermudes
    Department of Biology, Boston University, Massachusetts 02215, USA
    Ann N Y Acad Sci 503:515-27. 1987
    ..Additionally we report the identification and characterization of the polypeptide responsible for cross-reactivity with antitubulin antiserum...
  70. ncbi Tubulinlike protein from Spirochaeta bajacaliforniensis
    D Bermudes
    Department of Biology, Boston University, MA 02215, USA
    Ann N Y Acad Sci 503:515-27. 1987
    ..Additionally we report the identification and characterization of the polypeptide responsible for cross-reactivity with antitubulin antiserum...
  71. pmc Resistance to tick-borne spirochete challenge induced by Borrelia burgdorferi strains that differ in expression of outer surface proteins
    T J Kurtti
    Department of Entomology, University of Minnesota, St Paul 55108, USA
    Infect Immun 64:4148-53. 1996
    ..Destruction of spirochetes in ticks that fed on the hamsters vaccinated with the 297 bacterin suggests that antibodies to OspA and OspB may have been responsible, since the mutant did not induce this activity...
  72. pmc Phagocytosis of Borrelia burgdorferi and Treponema pallidum potentiates innate immune activation and induces gamma interferon production
    Meagan W Moore
    Department of Medicine, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3715, USA
    Infect Immun 75:2046-62. 2007
    ..burgdorferi by host cells and the ability of T. pallidum to avoid detection and uptake by virtue of its denuded outer membrane rather than by differences in surface lipoprotein expression...
  73. pmc Collateral effects of antibiotics: carbadox and metronidazole induce VSH-1 and facilitate gene transfer among Brachyspira hyodysenteriae strains
    Thaddeus B Stanton
    Pre Harvest Food Safety and Enteric Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, U S Department of Agriculture, Ames, Iowa 50010, USA
    Appl Environ Microbiol 74:2950-6. 2008
    ..hyodysenteriae strains. The results of these studies suggest that certain antibiotics may induce the production of prophage or prophage-like elements by intestinal bacteria and thereby impact intestinal microbial ecology...
  74. ncbi Evidence for a new type of outer membrane lipid in oral spirochete Treponema denticola. Functioning permeation barrier without lipopolysaccharides
    C P Schultz
    Robert Koch Institut, D 13353 Berlin, Nordufer 20, Germany
    J Biol Chem 273:15661-6. 1998
    ..The results suggest a different kind of permeation barrier that is built up by this particular OML of T. denticola, which is quite different from LPS normally essential for Gram-negative bacteria...
  75. ncbi Role of small mammals in the ecology of Borrelia burgdorferi in a peri-urban park in north coastal California
    C A Peavy
    Department of Environmental Science, Policy and Management University of California, Berkeley 94720, USA
    Exp Appl Acarol 21:569-84. 1997
    ..We conclude that brush rabbits and several species of rodents besides woodrats may contribute to the maintenance of B. burgdorferi because they harbour the spirochete and are fed upon by competent enzootic vectors...
  76. ncbi [First report of three culture confirmed human Lyme cases in Turkey]
    Erdal Polat
    İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Istanbul
    Mikrobiyol Bul 44:133-9. 2010
    ..These three cases supported the presence of Lyme disease in Turkey and indicated that the disease could present itself in various clinical pictures...
  77. ncbi Borrelia burgdorferi sensu stricto in yellow-necked mice and feeding Ixodes ricinus ticks in a forest habitat of west central Poland
    Jerzy Michalik
    Department of Animal Morphology, Adam Mickiewicz University, ul Umultowska 89, 61 614 Poznan, Poland
    J Med Entomol 42:850-6. 2005
    ..ricinus vector seems to be very low. The research provides additional information on the complexity of B. burgdorferi s.l. ecology in Europe, pointing to the importance of the local host community...
  78. ncbi Leptospirosis: an ignored cause of acute renal failure in Taiwan
    C W Yang
    Chang Gung Memorial Hospital, Department of Veterinary Medicine, National Taiwan University, Taipei, Republic of China
    Am J Kidney Dis 30:840-5. 1997
    ..Leptospirosis should be suspected in febrile patients with jaundice and renal failure when pathogens cannot be identified by traditional culture for microorganisms...
  79. ncbi Alleviation of renal and pulmonary injury by immunomodulation in leptospirosis: hamster model
    Kearkiat Praditpornsilpa
    Division of Nephrology, Department of Medicine, Faculty of Medicine, Chulalongkorn University Hospital, Bangkok, Thailand
    J Med Assoc Thai 89:S178-87. 2006
    ..The authors determined whether the current immunosuppressive agents could alleviate the inflammation and minimize the organ injury in hamster model...
  80. pmc Temporal changes in outer surface proteins A and C of the lyme disease-associated spirochete, Borrelia burgdorferi, during the chain of infection in ticks and mice
    T G Schwan
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    J Clin Microbiol 38:382-8. 2000
    ..Therefore, the temporal synthesis of OspC by spirochetes only in feeding ticks that were infected prior to the blood meal suggests that this surface protein is involved in transmission from tick to mammal but not from mammal to tick...
  81. ncbi Plaques of Alzheimer's disease originate from cysts of Borrelia burgdorferi, the Lyme disease spirochete
    Alan B MacDonald
    St Catherine of Siena Medical Center, Department of Pathology, 50 Rte 25 A, Smithtown, NY 11787, USA
    Med Hypotheses 67:592-600. 2006
    ..Parataxis tells us that what appears to be cause and effect in the couplet "amyloid plaque" merely by a proximity relationship may be "spurious causality" which is a cognitive dead end...
  82. pmc Complex population structure of Lyme borreliosis group spirochete Borrelia garinii in subarctic Eurasia
    Pär Comstedt
    Department of Molecular Biology, Umea University, Umea, Sweden
    PLoS ONE 4:e5841. 2009
    ....
  83. pmc Molecular characterization of a large Borrelia burgdorferi motility operon which is initiated by a consensus sigma70 promoter
    Y Ge
    Department of Microbiology and Immunology, West Virginia University, Robert C Byrd Health Sciences Center, Morgantown 26506 9177, USA
    J Bacteriol 179:2289-99. 1997
    ..These results indicate that these antigens are not favorable candidate reagents to be used in the diagnosis of Lyme disease...
  84. pmc Isolation, oxygen sensitivity, and virulence of NADH oxidase mutants of the anaerobic spirochete Brachyspira (Serpulina) hyodysenteriae, etiologic agent of swine dysentery
    T B Stanton
    National Animal Disease Center, Agricultural Research Service, Ames, Iowa 50010, USA
    Appl Environ Microbiol 65:5028-34. 1999
    ..These results provide evidence that NADH oxidase serves to protect B. hyodysenteriae cells against oxygen toxicity and that the enzyme, in that role, contributes to the pathogenic ability of the spirochete...
  85. pmc Dual flaA1 flaB1 mutant of Serpulina hyodysenteriae expressing periplasmic flagella is severely attenuated in a murine model of swine dysentery
    E L Rosey
    Veterinary Infectious Diseases, Pharmacia and Upjohn, Inc, Kalamazoo, Michigan 49001, USA
    Infect Immun 64:4154-62. 1996
    ..This strain represents the first spirochete engineered to contain specifically defined mutations in more than one genetic locus...
  86. pmc The chymotrypsin-like protease complex of Treponema denticola ATCC 35405 mediates fibrinogen adherence and degradation
    Caroline V Bamford
    Department of Oral and Dental Science, University of Bristol, Lower Maudlin St, Bristol BS1 2LY, United Kingdom
    Infect Immun 75:4364-72. 2007
    ..denticola cells, but not strain CKE (CTLP(-)) cells, were extended. These results suggest that interactions of T. denticola with fibrinogen, which may promote colonization and modulate hemostasis, are mediated principally by CTLP...
  87. ncbi Distribution and characterization of Borrelia burgdorferi isolates from Ixodes scapularis and presence in mammalian hosts in Ontario, Canada
    M G Morshed
    Laboratory Services, BC Centre for Disease Control, 655 West 12th Ave, Vancouver, British Columbia, Canada V5Z 4R4
    J Med Entomol 43:762-73. 2006
    ..Moreover, our findings of B. burgdorferi in I. scapularis reveal that people and domestic animals may be exposed to Lyme disease vector ticks, which have wide-ranging distribution in eastern and central Canada...
  88. ncbi [West African tick-borne relapsing fever]
    Y Lecompte
    Laboratoire de Paludologie, Institut de Recherche pour le Developpement, BP 1386, Dakar, Senegal
    Ann Biol Clin (Paris) 61:541-8. 2003
    ..In neurologic complications, the effective treatment is intravenous penicillin G or ceftriaxone. West African tick-borne relapsing fever must be systematically mentioned in case of fever in a patient returning from the endemic area...
  89. pmc Purine salvage pathways among Borrelia species
    Jonas Pettersson
    Rocky Mountain Laboratories, 903 S Fourth Street, Hamilton, MT 59840, USA
    Infect Immun 75:3877-84. 2007
    ..burgdorferi. This complete pathway for purine salvage in the relapsing fever spirochetes may contribute, in part, to these spirochetes achieving high cell densities in blood...
  90. pmc Genetic characteristics of Borrelia coriaceae isolates from the soft tick Ornithodoros coriaceus (Acari: Argasidae)
    M Hendson
    Division of Insect Biology, University of California, Berkeley, California 94720, USA
    J Clin Microbiol 38:2678-82. 2000
    ..The lack of concordance between different detection or isolation methods suggests that reliance upon a single technique may grossly underestimate the true prevalence of spirochetal infection in wild-caught O. coriaceus ticks...
  91. pmc Identification of homologs for thioredoxin, peptidyl prolyl cis-trans isomerase, and glycerophosphodiester phosphodiesterase in outer membrane fractions from Treponema pallidum, the syphilis spirochete
    D V Shevchenko
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Infect Immun 65:4179-89. 1997
    ..At the same time, however, these findings also raise important issues regarding the use of OM preparations for identifying rare OM proteins of T. pallidum...
  92. ncbi Migratory songbirds disperse ticks across Canada, and first isolation of the Lyme disease spirochete, Borrelia burgdorferi, from the avian tick, Ixodes auritulus
    Muhammad G Morshed
    Laboratory Services, British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada V5Z 4R4
    J Parasitol 91:780-90. 2005
    ..burgdorferi-infected ticks and increased risk of Lyme disease exposure...
  93. pmc Analysis of promoter elements involved in the transcriptional initiation of RpoS-dependent Borrelia burgdorferi genes
    Christian H Eggers
    Center for Microbial Pathogenesis, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3710, USA
    J Bacteriol 186:7390-402. 2004
    ..burgdorferi play a different role than has been described for E. coli...
  94. ncbi The prevalence of spirochete Borrelia burgdorferi sensu lato in ticks Ixodes ricinus and mosquitoes Aedes spp. within a selected recreational area in the city of Szczecin
    Danuta Kosik-Bogacka
    Department of Biology and Medical Parasitology, Pomeranian Medical University, 70 111 Szczecin, al Powstancow Wielkopolskich 72, Poland
    Ann Agric Environ Med 11:105-8. 2004
    ..The observed low values of the prevalence and infection intensity of female mosquitoes Aedes spp. compared to ticks suggest that the former do not pose a serious epidemiological threat in the spreading of Lyme disease...
  95. pmc Bloodmeal size and spirochete acquisition of Ornithodoros hermsi (Acari: Argasidae) during feeding
    Brandi N McCoy
    Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    J Med Entomol 47:1164-72. 2010
    ..These observations establish a basis for future studies to determine the minimum number of spirochetes required in the host's blood to allow O. hermsi to become persistently infected and transmit during subsequent bloodmeals...
  96. pmc RpoS is not central to the general stress response in Borrelia burgdorferi but does control expression of one or more essential virulence determinants
    Melissa J Caimano
    Center for Microbial Pathogenesis, University of Connecticut Health Center, 263 Farmington Ave, Farmington 06030 3710, USA
    Infect Immun 72:6433-45. 2004
    ....
  97. ncbi Epitope mapping of the outer surface protein A (OspA) of the spirochete Borrelia burgdorferi using a panel of monoclonal antibodies and lanthanide competition fluoroimmunoassay
    I G Kharitonenkov
    School of Basic Medicine, Lomonosov Moscow State University, Moscow, 119899, Russia
    Biochemistry (Mosc) 67:640-50. 2002
    ....
  98. pmc Archaeal-type lysyl-tRNA synthetase in the Lyme disease spirochete Borrelia burgdorferi
    M Ibba
    Department of Molecular Biophysics and Biochemistry, Cellular and Developmental Biology, Yale University, New Haven, CT 06520 8114, USA
    Proc Natl Acad Sci U S A 94:14383-8. 1997
    ..This difference between the lysyl-tRNA synthetases of spirochetes and their hosts may be readily exploitable for the development of anti-spirochete therapeutics...
  99. ncbi Intestinal spirochetes isolated from wild-living jackdaws, hooded crows and rooks (genus Corvus): provisionally designated "Brachyspira corvi" sp. nov
    Desiree S Jansson
    Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute SVA, SE 751 89 Uppsala, Sweden
    Anaerobe 14:287-95. 2008
    ..L gen. n. corvi, of a crow)...
  100. ncbi Prevalence of borrelia burgdorferi sensu lato infection among rodents and host-seeking ticks in South Carolina
    Kerry L Clark
    Department of Health Science, University of North Florida, Jacksonville 32224, USA
    J Med Entomol 39:198-206. 2002
    ..These findings add additional evidence that I. affinis and I. minor are potentially significant maintenance vectors of the spirochete...
  101. ncbi Isolation and characterization of chemotaxis mutants of the Lyme disease Spirochete Borrelia burgdorferi using allelic exchange mutagenesis, flow cytometry, and cell tracking
    MD A MOTALEB
    Department of Microbiology, West Virginia University, Morgantown, West Virginia, USA
    Methods Enzymol 422:421-37. 2007
    ..It should be possible to adapt these procedures to study other spirochete species, as well as other species of bacteria, especially those that have long generation times...

Research Grants73

  1. A unique approach to identify markers for congenital syphilis and neurosyphilis
    Nikhat Parveen; Fiscal Year: 2010
    ....
  2. Biophysics of the morphology and motility of Borrelia burgdorferi in diverse envi
    Charles W Wolgemuth; Fiscal Year: 2013
    ....
  3. Tick Midgut Proteins Critical for Borrelia Transmission
    Sukanya Narasimhan; Fiscal Year: 2010
    ..In this proposal we will identify tick midgut proteins that interact with Borrelia during spirochete growth and migration and examine the impact of this interaction on Borrelia growth in the tick and transmission to the murine host. ..
  4. Endemic maintenance of relapsing fever genotypes.
    Michael Bela Teglas; Fiscal Year: 2013
    ....
  5. GAMMA DELTA T CELLS IN LYME ARTHRITIS
    Ralph C Budd; Fiscal Year: 2011
    ..Understanding these interactions will improve our understanding of how ?? T cell regulate the immune response during infection and autoimmune conditions. ..
  6. Phagosomal Signals Shape Inflammatory Responses to Borrelia Burgdorferi
    Juan C Salazar; Fiscal Year: 2010
    ..abstract_text> ..
  7. Lyme neuroborreliosis pathogenesis in the rhesus monkey
    MARIO TOMAS PHILIPP; Fiscal Year: 2013
    ..burgdorferi, causes Lyme neuroborreliosis by killing neurons and oligodendrocytes. This research could lead to a new way of treating Lyme neuroborreliosis. ..
  8. Virulence Determinants of Borrelia burgdorferi
    Steven J Norris; Fiscal Year: 2013
    ..We anticipate that this project will continue to fuel new discoveries useful in improving the diagnosis, treatment, and prevention of Lyme borreliosis. ..
  9. Humoral responses to Lyme Borreliosis: A mouse model
    Nicole Baumgarth; Fiscal Year: 2012
    ..Such information is necessary to develop vaccines and treatments for this increasingly prevalent disease. ..
  10. RpoS Regulation of Borrelia burgdorferi Genes in vivo
    Justin D Radolf; Fiscal Year: 2013
    ..Our studies are based on the premise that improved understanding of the molecular mechanisms whereby Bb is transmitted by the arthropod vector will lead to new strategies for prevention and control of LD. ..
  11. RpoS-mediated virulence regulation in Borrelia burgdorferi
    JON SCOTT BLEVINS; Fiscal Year: 2013
    ..burgdorferi, which will help us identify potential therapeutic targets that can be used to prevent and/or treat Lyme disease. ..
  12. OspC regulation and pathogenic strategy of Borrelia burgdorferi
    Fang Ting Liang; Fiscal Year: 2013
    ..Tight regulation of gene expression is crucial for the pathogenic strategy of B. burgdorferi. The goal of the proposed study is to understand how B. burgdorferi regulates gene expression to meet its pathogenic strategy. ..
  13. Diagnostic Assays for early Lyme Borreliosis using in-vivo expressed antigens
    Michel Ledizet; Fiscal Year: 2012
    ..This application will determine whether a new assay for Lyme disease based on a novel panel of proteins expressed by spirochetes when they initially infect the skin accurately detects Lyme disease in its earliest stages. ..
  14. BORRELIA BURGDORFERI MITOGEN IN DEVELOPMENT OF ARTHRITIS
    Janis J Weis; Fiscal Year: 2013
    ..Preliminary studies suggest Lyme arthritis to be a versatile model for studying the in vivo role of microRNAs in inflammatory pathologies. ..
  15. The novel cross-linking of the flagellar hook protein of Borrelia burgdorferi
    NYLES CHARON; Fiscal Year: 2012
    ..By understanding this cross-linking, novel drugs can be developed to target this cross-linking and combat spirochete-caused diseases. ..
  16. Determinants of Borrelia Diversity and Population Structure
    ELISABETH BAUM; Fiscal Year: 2010
    ..The functional or neutralizing immune responses of the P. leucopus will also be characterized with the transgenic B. burgdorferi constructs that are isogenic, except for of the OspC type to be tested. ..
  17. 2009 Spirochetes, Biology of
    David A Haake; Fiscal Year: 2010
    ..Participants will include, but not be limited to, women and/or other minorities. ..
  18. Outer membrane proteins of Borrelia burgdorferi
    Darrin R Akins; Fiscal Year: 2013
    ..burgdorferi and Treponema pallidum, but could potentially be widely applicable against other important human pathogens, such as E. coli, Salmonella, Vibrio, Pseudomonas, Neisseria, Helicobacter and others. ..
  19. Virulence Determinants of Borrelia burgdorferi
    Steven J Norris; Fiscal Year: 2012
    ..We anticipate that this project will continue to fuel new discoveries useful in improving the diagnosis, treatment, and prevention of Lyme borreliosis. ..
  20. A New Cytokine-Based Immunoassay for the Diagnosis of Lyme Disease
    Linda K Bockenstedt; Fiscal Year: 2013
    ..As a strategy that complements current serologic tests, the whole blood cytokine assay will improve the combined sensitivity and specificity of LD diagnostics, especially in situations in which the serologic tests alone under-perform. ..
  21. Gene Expression Biomarkers for Diagnosis of Lyme Disease
    IRA S SCHWARTZ; Fiscal Year: 2012
    ..Once identified, this subset of classifier genes could be employed as the basis for development of a sensitive and specific gene-based diagnostic test for active B. burgdorferi infection. ..
  22. Comprehensive Analysis of Borrelia burgdorferi Adhesins
    John M Leong; Fiscal Year: 2013
    ..burgdorferi proteins that have unique biochemical activities and may be excellent candidates for development as vaccine candidates or targeting for therapeutic interventions. ..
  23. The RpoN-RpoS regulatory pathway in Borrelia burgdorferi
    Michael V Norgard; Fiscal Year: 2013
    ..Understanding the roles of these genes in transmission and pathogenesis processes could lead to new intervention strategies (vaccines, therapeutics) for Lyme disease. ..
  24. MOUSE MODEL OF LYME BORRELIOSIS
    STEPHEN WILLIAM BARTHOLD; Fiscal Year: 2012
    ..The proposed studies will incisively investigate the mechanisms of Borrelia burgdorferi persistence, which will help lead to more effective treatment. ..
  25. Natural History of Urban Leptospirosis
    Albert Icksang Ko; Fiscal Year: 2013
    ..The information gained from the project will lead to policy recommendations, which are needed to address leptospirosis in regions of the world where slum settlements are rapidly expanding. ..
  26. Biosynthesis and Function of Treponema denticola Lipooligosaccharide
    Hui Wu; Fiscal Year: 2013
    ....
  27. Mechanisms of Immune Evasion and Recognition in Human Syphilis
    ADRIANA RAQUEL CRUZ; Fiscal Year: 2013
    ....
  28. An Intracellular Niche for Borrelia burgdorferi
    Jonathan T Skare; Fiscal Year: 2013
    ..The overall goal of these studies is to determine the extent in which an intracellula locale contributes to borrelial persistence. ..
  29. Anti-polysaccharide antibody responses in humanized mice
    TIMOTHY L MANSER; Fiscal Year: 2013
    ..As such, we predict that the resulting HISmice will serve as far better models for the development of strategies for the diagnosis and treatment of human disease. ..
  30. RpoS Regulation of Borrelia burgdorferi Genes in vivo
    Justin D Radolf; Fiscal Year: 2012
    ..The underlying premise of these studies is that improved understanding of the molecular mechanisms whereby Bb is transmitted by the arthropod vector will lead to new prevention strategies. ..
  31. MOLECULAR GENETICS OF LYME ARTHRITIS SUSCEPTIBILITY
    Janis J Weis; Fiscal Year: 2013
    ..burgdorferi signaling in macrophages. ..
  32. Mutational analysis of the vlp/vsp antigenic variation system of the relapsing fe
    TROY MICHAEL BANKHEAD; Fiscal Year: 2013
    ..When applied, these results are expected to allow the targeting of this system in order to significantly reduce the ability of this pathogen to persist ad cause disease in the mammalian host. ..
  33. Borrelia burgdorferi tick phase genes and Lyme disease pathogenesis
    IRA S SCHWARTZ; Fiscal Year: 2013
    ..This will provide new insights into the natural history of B. burgdorferi infection in nature which, in turn, may have implications for prevention and treatment of human Lyme disease. ..
  34. A Lyme disease vaccine using newly identified in-vivo expressed antigens
    Michel Ledizet; Fiscal Year: 2013
    ..Phase II experiments will determine the optimal formulation and method of delivery. We will also examine whether antigens can be combined to provide full protection against Borrelia. ..
  35. Cellular processing and role of a surface antigen in borrelial pathogenesis
    Xiuli Yang; Fiscal Year: 2013
    ..This study will shed new light on the mechanism of spirochete pathogenesis and may also help the development of novel preventive measures against Lyme disease. ..
  36. The Role of BBI36/38 in Lyme Disease Pathogenesis
    Andrew J Nowalk; Fiscal Year: 2010
    ..The University of Pittsburgh and the mentoring support proposed here offer excellent tools for the development of a career in basic research. ..
  37. Disease Determinants of Urban Leptospirosis
    Albert Icksang Ko; Fiscal Year: 2013
    ....
  38. Outer membrane proteins of Borrelia burgdorferi
    Darrin R Akins; Fiscal Year: 2011
    ..coli, Salmonella, Vibrio, Pseudomonas, Neisseria, Helicobacter and others. ..
  39. Phagosomal Signals Shape Inflammatory Responses to B. Burgdorferi
    Juan C Salazar; Fiscal Year: 2013
    ....
  40. BORRELIA BURGDORFERI INTERACTIONS WITH IXODES SCAPULARIS
    Erol Fikrig; Fiscal Year: 2012
    ..If successful, these studies may lead to new strategies for the prevention of B. burgdorferi infection. This paradigm would also be applicable to other vector-borne pathogens of medical importance. ..
  41. Borella Gene Products Critical For Natural Infection Cycle
    Utpal Pal; Fiscal Year: 2013
    ..In sum, these studies will contribute to the understanding of the intriguing biology and infectivity of B. burgdorferi and hel develop novel preventive and therapeutic measures to combat infection. ..
  42. Real-time Imaging Analysis of Vector-borne Lyme Borreliosis Pathogenesis &Persis
    Linda K Bockenstedt; Fiscal Year: 2013
    ....
  43. The RpoN-RpoS regulatory pathway in Borrelia burgdorferi
    MICHAEL NORGARD; Fiscal Year: 2009
    ..Understanding the roles of these genes could lead to new intervention strategies (vaccines, therapeutics) for Lyme disease. ..
  44. A serologic assay to measure successful Lyme borreliosis antibiotic therapy
    Michel Ledizet; Fiscal Year: 2010
    ....
  45. Identifying Borrelia turicatae genes needed for colonization and transmission fro
    Job E Lopez; Fiscal Year: 2013
    ..These findings will have a broad impact enabling the development of therapeutics blocking transmission of this disease, and will increase our understanding of the adaptation and transmission mechanisms of vector-borne pathogens. ..
  46. Genetic analysis of the NKT cell response to Streptococcus pneumoniae
    JOSE LUIS VELA; Fiscal Year: 2010
    ..pneumoniae infection. Aim 2. To identify the consequences of glycosyl transferase loss of function mutation on direct and indirect antigen mediated Valpha 14iNKT cell activation in vivo and in vitro. ..
  47. Borrelia burdorferi Ebfc regulon
    Brian Stevenson; Fiscal Year: 2013
    ..Thus, our continued studies will focus efforts toward production of improved preventative and curative therapies for not only Lyme disease, but also many other significant human infectious diseases. ..
  48. Borrelia burgdorferi-glycosaminoglycan interactions and Lyme disease pathogenesis
    Nikhat Parveen; Fiscal Year: 2013
    ....
  49. 2012 Biology of Spirochetes Gordon Research Conference
    David A Haake; Fiscal Year: 2012
    ..The informal, discussion-oriented environment of the conference fosters valuable interactions between all participants. ..
  50. Protein-protein covalent bonding and Treponema motility
    Michael R Miller; Fiscal Year: 2013
    ..The results obtained should allow us to determine the role of FlgE cross-linking in Td pathogenesis. ..
  51. NDV-vectored Lyme disease vaccines
    Siba K Samal; Fiscal Year: 2013
    ..Overall, these studies will contribute to the development of preventive measures against multi-organ infection caused by B. burgdorferi. ..
  52. Exploring New Virulence Factors of the Oral Spirochete Treponema denticola
    Chunhao Li; Fiscal Year: 2013
    ....
  53. Role of a Borrelia burgdorferi fibronection-binding protein in Lyme Disease
    CATHERINE AYN BRISSETTE; Fiscal Year: 2010
    ..Data obtained in these studies will direct development of improved tools for treatment, prevention, and diagnosis of Lyme disease. ..
  54. Structure and Function of Treponema pallidum Lipoproteins
    Michael V Norgard; Fiscal Year: 2013
    ..pallidum during human infection. As such, our studies have the potential to elucidate the molecular bases of many new aspects of syphilis pathogenesis and, consequently, new potential avenues to thwart infection. ..
  55. CRASP-2 proteins of Lyme disease Borrelia
    Brian Stevenson; Fiscal Year: 2010
    ....
  56. Relapsing fever spirochete protein production within the vector
    Job E Lopez; Fiscal Year: 2013
    ..The results will also direct vaccine development against RF spirochetes, targeting bacterial Omps produced within the salivary glands, consequently neutralizing the bacteria as they enter the host. ..
  57. Autoimmunity to ECGF in Lyme disease and its post-infectious syndromes
    Allen C Steere; Fiscal Year: 2012
    ..Finally, this work has implications beyond LD, since it may create a new paradigm for how to identify and understand infection-induced autoimmunity with immune dysregulation in other diseases. ..
  58. Defining Borrelia Genes Required for Tick Infection and Transmission
    Aravinda M de Silva; Fiscal Year: 2012
    ..The goal of this proposal is to study how Lyme disease bacteria are transmitted by ticks. The work is applicable to developing novel vaccines that prevent ticks from acquiring or transmitting Lyme disease bacteria. ..
  59. Autoimmunity to ECGF in Lyme disease and its post-infectious syndromes
    Allen C Steere; Fiscal Year: 2013
    ..We anticipate that a test for these antibody responses will become a part of the clinical evaluation of LD. Such information will help physicians to treat patients with LD more appropriately and effectively. ..
  60. Genotypic Variation and B. burgdorferi Pathogenesis
    Ira Schwartz; Fiscal Year: 2009
    ..The proposed studies will shed light on the basis for symptom development in infected patients and on the factors contributing to dissemination of B. burgdorferi in humans. ..
  61. Understanding Unique Aspects of Motility and Chemotaxis in Borrelia burgdorferi
    Chunhao Li; Fiscal Year: 2011
    ..The goal of this project is to understand the basic biology of spirochete motility and chemotaxis, and their roles in the pathogenesis of the diseases. The obtained results may lead to new means of disease prevention and treatment. ..
  62. The Health Burden of A Recently Discovered Borrelia Spirochete
    PETER JAMES KRAUSE; Fiscal Year: 2012
    ..miyamotoi infection. ..
  63. Protective Role of Interleukin 10 in Neuroborreliosis
    Diego Cadavid; Fiscal Year: 2009
    ..Our preliminary results indicate that Interleukin 10 may be very important to protect the brain blood vessels from damage during this infection. We propose to study this in a mouse model of spirochetal brain infection. ..
  64. PERIPLASMIC FLAGELLA OF TREPONEMA DENTICOLA
    John Ruby; Fiscal Year: 1993
    ..Regions of homology will define conserved sequences among the spirochetes. These regions are likely to be necessary for PF structure and motility...
  65. GENE ANALYSIS OF SPIROCHETE PERIPLASMIC FLAGELLA
    NYLES CHARON; Fiscal Year: 1999
    ..They are especially relevant in that flagella and/or motility are implicated as a virulence factor for spirochetes and several other species of pathogenic bacteria. ..
  66. Pathogenesis of Lyme Borreliosis in the Rhesus Monkey
    MARIO PHILIPP; Fiscal Year: 2007
    ..burgdorferi and lipoprotein in inflammation and neurodegeneration, specifically, the expression and involvement of pertinent TLR in mediating cytokine production and apoptosis in the different cell combinations. ..
  67. The role of Borrelia burgdorferi chemotoxis in the pathogenesis of Lyme disease
    Md Motaleb; Fiscal Year: 2009
    ..The results of all studies will provide critical information on the pathogenesis of Lyme disease and could provide the foundation for novel approaches to prevent Lyme disease including Lyme associated arthritis. ..
  68. Anaplasma - B. burgorferi/endothelijm interactions
    DENNIS GRAB; Fiscal Year: 2006
    ..burgdorferi. The elucidation of the steps that explain the enhancement may allow for more intensive basic and clinical investigation into mechanisms of virulence for Lyme disease, HGE, and coinfections. ..
  69. Chronic Lyme Arthritis: Is this Autoimmunity?
    Brigitte Huber; Fiscal Year: 2005
    ..These experiments are designed to elucidate the mechanism of treatment resistant Lyme arthritis. ..
  70. MOLECULAR ANALYSIS OF TREPONEMAL MOTILITY GENES
    RONALD LIMBERGER; Fiscal Year: 2002
    ..Understanding the relationship of motility and cell structure to spirochete pathogenesis will result in development of therapeutics targeted towards inhibition of spirochete motility for prevention of human disease. ..
  71. Leptospira interrogans Len adhesins
    Brian Stevenson; Fiscal Year: 2009
    ..The aim of this project is to characterize a family of six leptospiral proteins we found to interact with human immune system regulators and extracellular matrix components, and may therefore play important roles in human leptospirosis. ..
  72. Role of Zonula Occludens Toxin in the Pathogenesis of Treponema denticola
    Chunhao Li; Fiscal Year: 2009
    ..Oral spirochete Treponema denticola is strongly associated with human periodontal and endodontic diseases. The goal of this proposal is to investigate the role of a putative Zot-like toxin in the pathogenesis of T. denticola. ..
  73. LYME DISEASE--DEVELOPMENT OF SKIN LESIONS AND ARTHRITIS
    GAIL HABICHT; Fiscal Year: 1993
    ..A rat model of Lyme arthritis will be investigated for the roles of LPS and PG in arthritogenesis...