rhizobium

Summary

Summary: A genus of gram-negative, aerobic, rod-shaped bacteria that produce nodules on the roots of leguminous plants. Members of this genus are common soil inhabitants.

Top Publications

  1. ncbi Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems
    Christel Schmeisser
    Biozentrum Klein Flottbek, Abteilung für Mikrobiologie und Biotechnologie, Universitat Hamburg, Germany
    Appl Environ Microbiol 75:4035-45. 2009
  2. ncbi Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
    Imogen A Sparkes
    School of Life Sciences, Oxford Brookes University, Oxford OX3 0BP, UK
    Nat Protoc 1:2019-25. 2006
  3. ncbi Replication-independent expression of genome components and capsid protein of brome mosaic virus in planta: a functional role for viral replicase in RNA packaging
    Padmanaban Annamalai
    Department of Plant Pathology, University of California, Riverside, CA 92521-0122, USA
    Virology 338:96-111. 2005
  4. ncbi Rhizobium alamii sp. nov., an exopolysaccharide-producing species isolated from legume and non-legume rhizospheres
    Odile Berge
    CEA, DSV, IBEB, SBVME, Laboratoire d Ecologie Microbienne de la Rhizosphère et d Environnements Extrêmes LEMiRE, F 13108 Saint Paul lez Durance, France
    Int J Syst Evol Microbiol 59:367-72. 2009
  5. ncbi Association of the Agrobacterium T-DNA-protein complex with plant nucleosomes
    Benoît Lacroix
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Proc Natl Acad Sci U S A 105:15429-34. 2008
  6. ncbi Biological systems of the host cell involved in Agrobacterium infection
    Vitaly Citovsky
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794, USA
    Cell Microbiol 9:9-20. 2007
  7. ncbi Symbiotic use of pathogenic strategies: rhizobial protein secretion systems
    William J Deakin
    LBMPS, University of Geneva, 30, quai Ernest Ansermet Sciences III, CH 1211 Geneve 4, Geneva, Switzerland
    Nat Rev Microbiol 7:312-20. 2009
  8. ncbi Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing and endomycorrhizal symbiotic associations
    A Boisson-Dernier
    , INRA-CNRS UMR215, Castanet-Tolosan, France
    Mol Plant Microbe Interact 14:695-700. 2001
  9. ncbi Early infection of scutellum tissue with Agrobacterium allows high-speed transformation of rice
    Seiichi Toki
    Plant Genetic Engineering Research Unit, National Institute of Agrobiological Sciences, 2 1 2 Kannondai, Tsukuba, Ibaraki 305 8602, Japan
    Plant J 47:969-76. 2006
  10. ncbi Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression system
    L K Johansen
    Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA
    Plant Physiol 126:930-8. 2001

Research Grants

  1. RHIZOBIAL LIPOPOLYSACCHARIDES ESSENTIAL FOR INFECTION
    Russell Carlson; Fiscal Year: 2009
  2. DnaK Protein Inhibitor Antimicrobials
    Michael Sturgess; Fiscal Year: 2003
  3. STRUCTURES OF ANTIBIOTICS AND RELATED COMPOUNDS
    KENNETH RINEHART; Fiscal Year: 1999
  4. LIPID A MODIFICATION SYSTEMS IN GRAM-NEGATIVE BACTERIA
    CHRISTIAN RAETZ; Fiscal Year: 2007
  5. RHIZOBIAL LIPOPOLYSACCHARIDES ESSENTIAL FOR INFECTION
    Russell Carlson; Fiscal Year: 2007
  6. MOLECULAR GENETICS OF RHIZOBIUM NODULATION PLASMIDS
    Graham Walker; Fiscal Year: 2009
  7. MOLECULAR GENETICS OF RHIZOBIUM NODULATION PLASMIDS
    Graham Walker; Fiscal Year: 2007
  8. MECHANISMS OF POLYSACCHARIDE SYNTHESIS BY S. PNEUMONIAE
    Janet Yother; Fiscal Year: 2005
  9. GENETICS OF DISEASE RESISTANCE IN ARABIDOPSIS THALIANA
    Roger Innes; Fiscal Year: 2003
  10. Genetic analysis of Cryptococcus neoformans virulence
    Joseph Heitman; Fiscal Year: 2007

Detail Information

Publications244 found, 100 shown here

  1. ncbi Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems
    Christel Schmeisser
    Biozentrum Klein Flottbek, Abteilung für Mikrobiologie und Biotechnologie, Universitat Hamburg, Germany
    Appl Environ Microbiol 75:4035-45. 2009
    b>Rhizobium sp. strain NGR234 is a unique alphaproteobacterium (order Rhizobiales) that forms nitrogen-fixing nodules with more legumes than any other microsymbiont. We report here that the 3...
  2. ncbi Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
    Imogen A Sparkes
    School of Life Sciences, Oxford Brookes University, Oxford OX3 0BP, UK
    Nat Protoc 1:2019-25. 2006
    ..These cells can be used to generate stable plant lines using a more time-consuming, cell culture technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months...
  3. ncbi Replication-independent expression of genome components and capsid protein of brome mosaic virus in planta: a functional role for viral replicase in RNA packaging
    Padmanaban Annamalai
    Department of Plant Pathology, University of California, Riverside, CA 92521-0122, USA
    Virology 338:96-111. 2005
    ..These results demonstrated that packaging of BMV genomic RNAs is not replication dependent whereas expression of a functional viral replicase plays an active role in increasing specificity of RNA packaging...
  4. ncbi Rhizobium alamii sp. nov., an exopolysaccharide-producing species isolated from legume and non-legume rhizospheres
    Odile Berge
    CEA, DSV, IBEB, SBVME, Laboratoire d Ecologie Microbienne de la Rhizosphère et d Environnements Extrêmes LEMiRE, F 13108 Saint Paul lez Durance, France
    Int J Syst Evol Microbiol 59:367-72. 2009
    ..16S rRNA gene sequence analysis showed that the isolates are closely related to the strains Rhizobium sp. YAS34 and USDA 1920, respectively isolated from sunflower roots and Medicago ruthenica nodules...
  5. ncbi Association of the Agrobacterium T-DNA-protein complex with plant nucleosomes
    Benoît Lacroix
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Proc Natl Acad Sci U S A 105:15429-34. 2008
    ..Furthermore, this nucleosome-associated ternary complex can bind another bacterial virulence factor, VirF, which is an F-box protein known to target both VirE2 and VIP1 for proteasomal degradation and uncoat the T-DNA...
  6. ncbi Biological systems of the host cell involved in Agrobacterium infection
    Vitaly Citovsky
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794, USA
    Cell Microbiol 9:9-20. 2007
    ....
  7. ncbi Symbiotic use of pathogenic strategies: rhizobial protein secretion systems
    William J Deakin
    LBMPS, University of Geneva, 30, quai Ernest Ansermet Sciences III, CH 1211 Geneve 4, Geneva, Switzerland
    Nat Rev Microbiol 7:312-20. 2009
    ..One group of proteins secreted by rhizobia have homologues in bacterial pathogens and may have been co-opted by rhizobia for symbiotic purposes...
  8. ncbi Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing and endomycorrhizal symbiotic associations
    A Boisson-Dernier
    , INRA-CNRS UMR215, Castanet-Tolosan, France
    Mol Plant Microbe Interact 14:695-700. 2001
    ..Our results suggest that M. truncatula hairy roots represent a particularly attractive system with which to study endosymbiotic associations in transgenically modified roots...
  9. ncbi Early infection of scutellum tissue with Agrobacterium allows high-speed transformation of rice
    Seiichi Toki
    Plant Genetic Engineering Research Unit, National Institute of Agrobiological Sciences, 2 1 2 Kannondai, Tsukuba, Ibaraki 305 8602, Japan
    Plant J 47:969-76. 2006
    ..Our new system should reduce the somaclonal variation accompanying prolonged culture of rice cells in the dedifferentiated state and facilitate the molecular breeding of rice...
  10. ncbi Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression system
    L K Johansen
    Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA
    Plant Physiol 126:930-8. 2001
    ..They also indicate that RNA silencing may be a significant limitation to expression of genes in the Agrobacterium-mediated transient assay but that this limitation can be overcome by using RNA silencing suppressors...
  11. ncbi The mosaic structure of the symbiotic plasmid of Rhizobium etli CFN42 and its relation to other symbiotic genome compartments
    Victor Gonzalez
    Centro de Investigacion Sobre Fijacion de Nitrogeno, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico 62210
    Genome Biol 4:R36. 2003
    ..We report the complete sequence of the symbiotic plasmid of Rhizobium etli CFN42, a microsymbiont of beans, and a comparison with other SGC sequences available.
  12. ncbi T-DNA binary vectors and systems
    Lan-Ying Lee
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA
    Plant Physiol 146:325-32. 2008
  13. ncbi Rhizobium selenireducens sp. nov.: a selenite-reducing alpha-Proteobacteria isolated from a bioreactor
    W J Hunter
    United States Department of Agriculture Agricultural Research Service, 2150 D Centre Avenue, Fort Collins, CO 80526 8119, USA
    Curr Microbiol 55:455-60. 2007
    ..16S rRNA gene-sequence alignment identified the isolate as a Rhizobium sp. belonging to the Rhizobium clade, which includes R. daejeonense, R. giardinii, R. undicola, R...
  14. ncbi Plant proteins involved in Agrobacterium-mediated genetic transformation
    Stanton B Gelvin
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 1392, USA
    Annu Rev Phytopathol 48:45-68. 2010
    ....
  15. ncbi NopL, an effector protein of Rhizobium sp. NGR234, thwarts activation of plant defense reactions
    Alexander V Bartsev
    Laboratoire de Biologie Moleculaire des Plantes Superieures, Sciences III, Universite de Geneve, 1211 Geneve 4, Switzerland
    Plant Physiol 134:871-9. 2004
    ..Type III secretion systems have been found in Rhizobium species that form symbiotic, nitrogen-fixing associations with legumes. One such bacterium, Rhizobium sp...
  16. ncbi A case of promiscuity: Agrobacterium's endless hunt for new partners
    Benoît Lacroix
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Trends Genet 22:29-37. 2006
    ..Moreover, better knowledge of the DNA-transfer mechanisms from bacteria to eukaryotic organisms can also help in understanding horizontal gene transfer--a driving force throughout biological evolution...
  17. ncbi Manipulation of auxin transport in plant roots during Rhizobium symbiosis and nematode parasitism
    Wim Grunewald
    Department Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, B 9000 Gent, Belgium
    Plant Cell 21:2553-62. 2009
    ..In this review, we focus on recent findings in auxin transport mechanisms during plant development and on how plant symbionts and parasites have evolved to manipulate these mechanisms for their own purposes...
  18. ncbi Role of BacA in lipopolysaccharide synthesis, peptide transport, and nodulation by Rhizobium sp. strain NGR234
    Silvia Ardissone
    Laboratoire de Biologie Moleculaire des Plantes Superieures, Universite de Geneve, 30 Quai Ernest Ansermet, Sciences III, 1211 Geneva 4, Switzerland
    J Bacteriol 193:2218-28. 2011
    ..We investigated the role of BacA in Rhizobium species strain NGR234 by mutating the bacA gene...
  19. ncbi Conservation of plasmid-encoded traits among bean-nodulating Rhizobium species
    Susana Brom
    Programa de Genetica Molecular de Plasmidos Bacterianos, Centro de Investigacion Sobre Fijacion de Nitrogeno, UNAM, Cuernavaca, Morelos, Mexico
    Appl Environ Microbiol 68:2555-61. 2002
    b>Rhizobium etli type strain CFN42 contains six plasmids...
  20. ncbi Lifestyle alternatives for rhizobia: mutualism, parasitism, and forgoing symbiosis
    R Ford Denison
    Department of Agronomy and Range Science, University of California at Davis, 1 Shields Avenue, Davis, CA 95616, USA
    FEMS Microbiol Lett 237:187-93. 2004
    ..Nonsymbiotic strains are unable to infect legumes at all. Why have rhizobium strains with one of these three strategies not displaced the others? A symbiotic (mutualistic or parasitic) ..
  21. ncbi Lipopolysaccharides from Legionella and Rhizobium stimulate mouse bone marrow granulocytes via Toll-like receptor 2
    Robert Girard
    Lymphocyte Development, URA-1961 of the National Center for Scientific Research, Pasteur Institute, Paris, France
    J Cell Sci 116:293-302. 2003
    ..from bacteria that do not belong to the Enterobacteriaceae, we show that in bone marrow cells (BMCs) the LPS of Rhizobium species Sin-1 and of three strains of Legionella pneumophila require TLR2 rather than TLR4 to elicit the ..
  22. ncbi Analysis of core genes supports the reclassification of strains Agrobacterium radiobacter K84 and Agrobacterium tumefaciens AKE10 into the species Rhizobium rhizogenes
    Encarna Velázquez
    Departamento de Microbiologia y Genetica, Edificio Departamental de Biología, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain
    Syst Appl Microbiol 33:247-51. 2010
    ..phylogenetic analysis of the chromosomal genes rrs, atpD and recA showed that they should be reclassified into Rhizobium rhizogenes...
  23. ncbi Rhizobium alkalisoli sp. nov., isolated from Caragana intermedia growing in saline-alkaline soils in the north of China
    Yang Li Lu
    State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China
    Int J Syst Evol Microbiol 59:3006-11. 2009
    ..7 and 99.5 % sequence similarities with those of Rhizobium huautlense SO2(T) and Rhizobium galegae USDA 4128(T), respectively...
  24. ncbi An embryogenic suspension cell culture system for Agrobacterium-mediated transformation of citrus
    M Dutt
    Citrus Research and Education Center, University of Florida IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850, USA
    Plant Cell Rep 29:1251-60. 2010
    ..This protocol can create an avenue for insertion of useful traits into any polyembryonic citrus cultivar that can be established as embryogenic cell suspension cultures, including popular specialty mandarins and seedless cultivars...
  25. ncbi Stabilizing mechanisms in a legume-rhizobium mutualism
    Katy D Heath
    Department of Plant Biology, University of Minnesota, St Paul, Minnesota 55108, USA
    Evolution 63:652-62. 2009
    ..partner choice and sanctioning may play roles in preventing the invasion of less-beneficial rhizobia in legume-rhizobium mutualisms. The importance of these mechanisms in natural communities, however, remains unclear...
  26. ncbi Involvement of targeted proteolysis in plant genetic transformation by Agrobacterium
    Tzvi Tzfira
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794 5215, USA
    Nature 431:87-92. 2004
    ..The critical role of proteasomal degradation in Agrobacterium-mediated genetic transformation was also evident from inhibition of T-DNA expression by a proteasomal inhibitor...
  27. ncbi Curdlan and other bacterial (1-->3)-beta-D-glucans
    M McIntosh
    Department of Biochemistry, La Trobe University, Bundoora, 3086 Victoria, Australia
    Appl Microbiol Biotechnol 68:163-73. 2005
    ....
  28. ncbi Surface polysaccharide involvement in establishing the rhizobium-legume symbiosis
    Nicolas Fraysse
    Laboratoire des IMRCP, UMR5623 UPS/CNRS, 118 route de Narbonne, F-31062 Toulouse, France
    Eur J Biochem 270:1365-80. 2003
    ..Their chemistry is important for structural recognition as well as for physico-chemical properties...
  29. ncbi Conjugative transfer of p42a from rhizobium etli CFN42, which is required for mobilization of the symbiotic plasmid, is regulated by quorum sensing
    Cristina Tun-Garrido
    Programa de Genetica Molecular de Plasmidos Bacterianos, Centro de Investigacion Sobre Fijacion de Nitrogeno, UNAM, Cuernavaca, Morelos, Mexico
    J Bacteriol 185:1681-92. 2003
    b>Rhizobium etli CFN42 contains six plasmids. Only one of them, p42a, is self-conjugative at high frequency. This plasmid is strictly required for mobilization of the symbiotic plasmid (pSym)...
  30. ncbi Agrobacterium tumefaciens and Agrobacterium rhizogenes transformed roots of the parasitic plant Triphysaria versicolor retain parasitic competence
    Alexey Tomilov
    Department of Plant Sciences, University of California Davis, Davis, CA 95616, USA
    Planta 225:1059-71. 2007
    ..Transgenic roots also remained competent to form haustoria in response to purified inducing factors. These transformation systems will allow an in planta assessment of genes predicted to function in plant parasitism...
  31. ncbi A revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al. 1998 as new combinations: Rhizobium radiobacter, R. rhizogenes, R. rubi,
    J M Young
    Landcare Research, Auckland, New Zealand
    Int J Syst Evol Microbiol 51:89-103. 2001
    b>Rhizobium, Agrobacterium and Allorhizobium are genera within the bacterial family Rhizobiaceae, together with Sinorhizobium. The species of Agrobacterium, Agrobacterium tumefaciens (syn...
  32. ncbi The VirE3 protein of Agrobacterium mimics a host cell function required for plant genetic transformation
    Benoît Lacroix
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    EMBO J 24:428-37. 2005
    ....
  33. ncbi Rhizavidin from Rhizobium etli: the first natural dimer in the avidin protein family
    Satu H Helppolainen
    Institute of Medical Technology, University of Tampere, FI 33014 Tampere, Finland
    Biochem J 405:397-405. 2007
    b>Rhizobium etli CFN42 is a symbiotic nitrogen-fixing bacterium of the common bean Phaseolus vulgaris. The symbiotic plasmid p42d of R. etli comprises a gene encoding a putative (strept)avidin-like protein, named rhizavidin...
  34. ncbi Rhizobium meliloti fixGHI sequence predicts involvement of a specific cation pump in symbiotic nitrogen fixation
    D Kahn
    Laboratoire de Biologie Moleculaire des relations Plantes Microorganismes, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Castanet Tolosan, France
    J Bacteriol 171:929-39. 1989
    We present genetic and structural analyses of a fix operon conserved among rhizobia, fixGHI from Rhizobium meliloti. The nucleotide sequence of the operon suggests it may contain a fourth gene, fixS...
  35. ncbi Variability in the level of RNA silencing suppression caused by triple gene block protein 1 (TGBp1) from various potexviruses during infection
    Hiroko Senshu
    Department of Agricultural and Environmental Biology, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    J Gen Virol 90:1014-24. 2009
    ..Moreover, we investigated the effect of TGBp1 encoded by Plantago asiatica mosaic virus (PlAMV), which exhibited a strong suppressor activity, on the accumulation of microRNA, virus genomic RNA and virus-derived small interfering RNAs...
  36. ncbi rosR, a determinant of nodulation competitiveness in Rhizobium etli
    M A Bittinger
    Cellular and Molecular Biology Program, University of Wisconsin Madison 53706, USA
    Mol Plant Microbe Interact 10:180-6. 1997
    We previously described a Tn5 mutant of Rhizobium etli strain CE3, designated CE3003, that is decreased in nodulation competitiveness, reduced in competitive growth in the rhizosphere, and has a hydrophobic cell surface (R. S. Araujo, E...
  37. ncbi RepA negatively autoregulates the transcription of the repABC operon of the Rhizobium etli symbiotic plasmid basic replicon
    M A Ramírez-Romero
    Programa de Evolución Molecular, Centro de Investigacion Sobre Fijacion de Nitrogeno, Universidad Nacional Autonoma de Mexico, Apartado Postal 565 A, Cuernavaca, Morelos, Mexico
    Mol Microbiol 42:195-204. 2001
    The basic replicon of Rhizobium etli CE3, like other members of the repABC plasmid family, is constituted by the repABC operon. RepC is essential for replication, and RepA and RepB play a role in plasmid segregation...
  38. ncbi R gene-controlled host specificity in the legume-rhizobia symbiosis
    Shengming Yang
    Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546, USA
    Proc Natl Acad Sci U S A 107:18735-40. 2010
    ..This study suggests that establishment of a root nodule symbiosis requires the evasion of plant immune responses triggered by rhizobial effectors...
  39. ncbi The mid genes of Rhizobium sp strain TAL1145 are required for degradation of mimosine into 3-hydroxy-4-pyridone and are inducible by mimosine
    D Borthakur
    Department of Molecular Biosciences and Bioengineering, University of Hawaii, 1955 E West Road, Ag Sci 218, Honolulu, HI 96822, USA
    Microbiology 149:537-46. 2003
    ..The leucaena-nodulating Rhizobium sp. strain TAL1145 degrades mimosine (Mid(+)) and utilizes it as a source of carbon and nitrogen...
  40. ncbi Functional analysis of the type 3 effector nodulation outer protein L (NopL) from Rhizobium sp. NGR234: symbiotic effects, phosphorylation, and interference with mitogen-activated protein kinase signaling
    Ling Zhang
    State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat Sen University, Guangzhou 510006, China
    J Biol Chem 286:32178-87. 2011
    ..Nodulation outer protein L (NopL) of Rhizobium sp. strain NGR234 is a Rhizobium-specific type 3 effector...
  41. ncbi Bathy phytochromes in rhizobial soil bacteria
    Gregor Rottwinkel
    Institute of Botany, Faculty of Chemistry and Biosciences, Karlsruhe Institute of Technology, Kaiserstr 2, 76131 Karlsruhe, Germany
    J Bacteriol 192:5124-33. 2010
    ..b>Rhizobium leguminosarum 3841, Rhizobium etli CIAT652, and Azorhizobium caulinodans ORS571 contain a single phytochrome of ..
  42. ncbi Rhizobial measures to evade host defense strategies and endogenous threats to persistent symbiotic nitrogen fixation: a focus on two legume-rhizobium model systems
    Kazuhiko Saeki
    Department of Biological Sciences, Faculty of Science, Nara Women s University, Kitauoya Nishimachi, Nara, Japan
    Cell Mol Life Sci 68:1327-39. 2011
    The establishment and maintenance of rhizobium-legume symbioses require a sequence of highly regulated and coordinated events between the organisms...
  43. ncbi Genetic dissection of Rhizobium-induced infection and nodule organogenesis in pea based on ENOD12A and ENOD5 expression analysis
    E A Dolgikh
    All Russia Research Institute for Agricultural Microbiology ARRIAM, St Petersburg, Russia
    Plant Biol (Stuttg) 13:285-96. 2011
    ..Activation of PsENOD12a only requires components involved in early steps of signalling and can be considered as a marker of early symbiotic events preceding infection...
  44. ncbi Agroinfiltration as a technique for rapid assays for evaluating candidate insect resistance transgenes in plants
    Brian Michael Leckie
    Department of Plant Sciences, University of Tennessee, 2431 Joe Johnson Dr, 252 Ellington Plant Sciences Bldg, Knoxville, TN 37996 4561, USA
    Plant Cell Rep 30:325-34. 2011
    ..This agroinfiltration-insect bioassay procedure can give a rapid assessment of insect resistance significantly decreasing the time needed for evaluation of candidate genes...
  45. ncbi Agrobacterium-mediated transient expression in citrus leaves: a rapid tool for gene expression and functional gene assay
    Jose F L Figueiredo
    Plant Pathology Department, University of Florida, Gainesville, FL, USA
    Plant Cell Rep 30:1339-45. 2011
    ..Transient expression in citrus has the potential for aiding in the development of new disease defense strategies in citrus...
  46. ncbi Renilla luciferase-based quantitation of Potato virus A infection initiated with Agrobacterium infiltration of N. benthamiana leaves
    K Eskelin
    Department of Applied Chemistry and Microbiology, PO Box 27, FIN 00014 University of Helsinki, Finland
    J Virol Methods 164:101-10. 2010
    ..However, a subtle increase in the mRNA level led to pronounced changes in Rluc activity. PVA CP accumulation was quantitated by enzyme-linked immunosorbent assay. The increase in Rluc activity correlated closely with virus accumulation...
  47. ncbi Studies on the biodegradation of fosfomycin: growth of Rhizobium huakuii PMY1 on possible intermediates synthesised chemically
    John W McGrath
    School of Biology and Biochemistry, The Queen s University of Belfast, Medical Biology Centre, 97 Lisburn Rd, Belfast, BT9 7BL, Northern Ireland
    Org Biomol Chem 7:1944-53. 2009
    ..The P(i) released from (1R,2R)-1,2-dihydroxypropylphosphonic acid labelled with one oxygen-18 in the PO3H2 group did not stay long enough in the cells to allow complete exchange of 18O for 16O by enzymic turnover...
  48. ncbi Detection of two loci involved in (1-->3)-beta-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene
    S J Stasinopoulos
    Department of Biochemistry and Department of Microbiology, La Trobe University, Bundoora 3083, Australia
    Glycobiology 9:31-41. 1999
    ..Whatever the determinants of the linkage specificity of these beta-glucan synthases might be, these results raise the possibility that (1-->3)-beta-glucans and (1-->4)-beta-glucans are formed by related catalytic polypeptides...
  49. ncbi High catalase production by Rhizobium radiobacter strain 2-1
    Mami Nakayama
    S G Laboratory Co, 2 35 Ohorikoen, Chuo Ku, Fukuoka 810 0051, Japan
    J Biosci Bioeng 106:554-8. 2008
    ..Strain 2-1 was identified as Rhizobium radiobacter (formerly known as Agrobacterium tumefaciens) on the basis of both phenotypic and genotypic ..
  50. ncbi Intergenomic epistasis and coevolutionary constraint in plants and rhizobia
    Katy D Heath
    Department of Plant Biology, University of Minnesota, St Paul, Minnesota 55108, USA
    Evolution 64:1446-58. 2010
    ..natural populations, I studied the symbiotic fitness benefits for both partners in 108 plant maternal family by rhizobium strain combinations...
  51. ncbi Exo-oligosaccharides of Rhizobium sp. strain NGR234 are required for symbiosis with various legumes
    Christian Staehelin
    State Key Laboratory of Biocontrol, School of Life Sciences, SunYat Sen Zhongshan University, Guangzhou 510275, China
    J Bacteriol 188:6168-78. 2006
    ..An acidic EPS from Rhizobium sp...
  52. ncbi Dissection of symbiosis and organ development by integrated transcriptome analysis of lotus japonicus mutant and wild-type plants
    Niels Høgslund
    Centre for Carbohydrate Recognition and Signalling, MBI, Aarhus University, Aarhus C, Denmark
    PLoS ONE 4:e6556. 2009
    Genetic analyses of plant symbiotic mutants has led to the identification of key genes involved in Rhizobium-legume communication as well as in development and function of nitrogen fixing root nodules...
  53. ncbi Rhizobium lusitanum sp. nov. a bacterium that nodulates Phaseolus vulgaris
    Angel Valverde
    , IRNASA-CSIC, Salamanca, Spain
    Int J Syst Evol Microbiol 56:2631-7. 2006
    ..to phylogenetic analysis of the 16S rRNA gene sequence, the strains from this study belong to the genus Rhizobium, with Rhizobium rhizogenes and Rhizobium tropici as the closest related species, with 99.9 and 99...
  54. ncbi Rhizobium cellulosilyticum sp. nov., isolated from sawdust of Populus alba
    Paula García Fraile
    Departamento de Microbiologia y Genetica, Universidad de Salamanca, Spain
    Int J Syst Evol Microbiol 57:844-8. 2007
    ..sources, two strains were isolated from pulverized decaying wood of Populus alba and classified in the genus Rhizobium on basis of their almost complete 16S rRNA gene sequences...
  55. ncbi Transcriptional profiling of ESTs responsive to Rhizobium vitis from 'Tamnara' grapevines (Vitis sp.)
    Youn Jung Choi
    Fruits Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 440 706, Republic of Korea
    J Plant Physiol 167:1084-92. 2010
    Genes related with defense responses were screened from the cDNA library constructed with Rhizobium vitis-inoculated or salicylic acid (SA)-treated 'Tamnara' grapevine (Vitis sp.) leaves...
  56. ncbi Rhizobium hainanense sp. nov., isolated from tropical legumes
    W X Chen
    Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing, People s Republic of China
    Int J Syst Bacteriol 47:870-3. 1997
    ..group isolated from leguminous plants in Hainan Province, a tropical region of China, is proposed as a new Rhizobium species on the basis of 16S rRNA gene sequencing. DNA-DNA hybridization, and phenotypic characterization...
  57. ncbi Role of symbiotic auxotrophy in the Rhizobium-legume symbioses
    Jurgen Prell
    John Innes Centre, Norwich Research Park, Norwich, United Kingdom
    PLoS ONE 5:e13933. 2010
    b>Rhizobium leguminosarum bv...
  58. ncbi Renaming of Agrobacterium larrymoorei Bouzar and Jones 2001 as Rhizobium larrymoorei (Bouzar and Jones 2001) comb. nov
    J M Young
    Landcare Research, Private Bag 92170, Auckland, New Zealand
    Int J Syst Evol Microbiol 54:149. 2004
    In conformity with the nomenclature of the genus Rhizobium published by Young et al. (Int J Syst Evol Microbiol 51, 89-103, 2001), it is proposed that Agrobacterium larrymoorei be named as Rhizobium larrymoorei comb. nov.
  59. ncbi Molecular characterization of grapevine plants transformed with GFLV resistance genes: I
    Giorgio Gambino
    , Via Leonardo da Vinci 44, I-10095 Grugliasco (Turin, Italy
    Plant Cell Rep 24:655-62. 2005
    ..Specific mRNA was present at variable expression levels; some lines accumulated the coat protein while in others the protein was not detectable by ELISA...
  60. ncbi Rhizobium daejeonense sp. nov. isolated from a cyanide treatment bioreactor
    Zhe-Xue Quan
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea
    Int J Syst Evol Microbiol 55:2543-9. 2005
    ..inferred from comparative 16S rRNA gene sequences indicated that the isolates fall in a sublineage of the genus Rhizobium comprising the type strains of Rhizobium giardinii, Rhizobium radiobacter, Rhizobium rubi, Rhizobium larrymoorei,..
  61. ncbi Cloning and characterization of a Rhizobium meliloti homolog of the Escherichia coli cell division gene ftsZ
    W Margolin
    Department of Biological Sciences, Stanford University, California 94305 5020
    J Bacteriol 173:5822-30. 1991
    ..To begin our studies of division arrest during differentiation of Rhizobium meliloti bacteroids, we isolated a R. meliloti ftsZ homolog, ftsZRm...
  62. ncbi Molecular cloning and nucleotide sequence of the Rhizobium phaseoli recA gene
    J Michiels
    F A Janssens Laboratory of Genetics, University of Leuven, Heverlee, Belgium
    Mol Gen Genet 228:486-90. 1991
    A recombinant lambda phage carrying the recA gene of Rhizobium phaseoli was isolated from a R. phaseoli genomic library by complementation of the Fec- phenotype of the recombinant phage in Escherichia coli. When expressed in E...
  63. ncbi Stable transformation and direct regeneration in Coffea canephora P ex. Fr. by Agrobacterium rhizogenes mediated transformation without hairy-root phenotype
    Vinod Kumar
    Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore 570 020, Karnataka, India
    Plant Cell Rep 25:214-22. 2006
    ..The study clearly demonstrates that A. rhizogenes can be used for delivering transgenes into tree species like Coffea using binary vectors with Agrobacterium tumefaciens T-DNA borders...
  64. ncbi Phylogenetic analysis of the genera Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium on the basis of 16S rRNA gene and internally transcribed spacer region sequences
    Soon Wo Kwon
    Korean Agricultural Culture Collection KACC, Genetic Resources Division, National Institute of Agricultural Biotechnology, Rural Development Administration RDA, Suwon 441 707, Korea
    Int J Syst Evol Microbiol 55:263-70. 2005
    ..relationships between these Korean isolates and reference strains of the genera Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium were analysed using their 16S rRNA gene and internally transcribed spacer (ITS) region ..
  65. ncbi Rhizobium meliloti exoG and exoJ mutations affect the exoX-exoY system for modulation of exopolysaccharide production
    J W Reed
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    J Bacteriol 173:3776-88. 1991
    ..this region reveals that it contains two open reading frames, called exoX and exoY, which have homologs in other Rhizobium species...
  66. ncbi Evidence that the Rhizobium regulatory protein RirA binds to cis-acting iron-responsive operators (IROs) at promoters of some Fe-regulated genes
    K H Yeoman
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Microbiology 150:4065-74. 2004
    Mutations in rirA of Rhizobium have been shown to deregulate expression of several genes that are normally repressed by iron...
  67. ncbi Cascade regulation of nif gene expression in Rhizobium meliloti
    M David
    Laboratoire de Biologie Moléculaire des Relations Plantes Microorganismes CNRS INRA, BP27, Castanet Tolosan, France
    Cell 54:671-83. 1988
    We report the discovery of two genes from Rhizobium meliloti, fixL and fixJ, which are positive regulators of symbiotic expression of diverse nitrogen fixation (nif and fix) genes...
  68. ncbi Analysis of pss genes of Rhizobium leguminosarum required for exopolysaccharide synthesis and nodulation of peas: their primary structure and their interaction with psi and other nodulation genes
    D Borthakur
    John Innes Institute, Norwich, UK
    Mol Gen Genet 213:155-62. 1988
    Strains of Rhizobium leguminosarum (R.l.) biovar viciae containing pss mutations fail to make the acidic exopolysaccharides (EPS) and are unable to nodulate peas...
  69. ncbi Efficient production of Agrobacterium rhizogenes-transformed roots and composite plants for studying gene expression in coffee roots
    E Alpizar
    , , UMR-DGPC, , Montpellier, France
    Plant Cell Rep 25:959-67. 2006
    ..These results suggest that composite plants constitute a powerful tool for studying nematode resistance genes...
  70. ncbi Engineering the nifH promoter region and abolishing poly-beta-hydroxybutyrate accumulation in Rhizobium etli enhance nitrogen fixation in symbiosis with Phaseolus vulgaris
    Humberto Peralta
    , , , Cuernavaca, Morelos CP62271, Mexico
    Appl Environ Microbiol 70:3272-81. 2004
    b>Rhizobium etli, as well as some other rhizobia, presents nitrogenase reductase (nifH) gene reiterations. Several R...
  71. ncbi Identification and characterization of the Rhizobium meliloti ntrC gene: R. meliloti has separate regulatory pathways for activation of nitrogen fixation genes in free-living and symbiotic cells
    W W Szeto
    J Bacteriol 169:1423-32. 1987
    We show here that Rhizobium meliloti, the nitrogen-fixing endosymbiont of alfalfa (Medicago sativa), has a regulatory gene that is structurally homologous to previously characterized ntrC genes in enteric bacteria...
  72. ncbi Quorum sensing in Rhizobium sp. strain NGR234 regulates conjugal transfer (tra) gene expression and influences growth rate
    Xuesong He
    Department of Biology, Indiana University, 1001 E. 3rd Street, Bloomington, IN 47405, USA
    J Bacteriol 185:809-22. 2003
    b>Rhizobium sp. strain NGR234 forms symbiotic, nitrogen-fixing nodules on a wide range of legumes via functions largely encoded by the plasmid pNGR234a...
  73. ncbi Expression of human acidic fibroblast growth factor in Nicotiana benthamiana with a potato-virus-X-based binary vector
    Jingying Liu
    Institute of Genetics and Cytology, Northeast Normal University, 5268 Renmin Street, Changchun 130024, People s Republic of China
    Biotechnol Appl Biochem 48:143-7. 2007
    ..haFGF was then purified on heparin-Sepharose CL-6B. The purified haFGF could stimulate the growth of NIH 3T3 cells, suggesting that the recombinant haFGF expressed via PVX viral vector in N. benthamiana was active biologically...
  74. ncbi Rhizobium oryzae sp. nov., isolated from the wild rice Oryza alta
    Guixiang Peng
    College of Resources and Environment, South China Agricultural University, Guangzhou 510642, PR China
    Int J Syst Evol Microbiol 58:2158-63. 2008
    ..species Oryza alta, eight novel isolates were obtained from surface-sterilized roots and classified in the genus Rhizobium on the basis of almost-complete 16S rRNA gene sequence analysis...
  75. ncbi A genome-wide gain-of function analysis of rice genes using the FOX-hunting system
    Hidemitsu Nakamura
    Division of Genome and Biodiversity Research, NationalInstitute of Agrobiological Sciences, 2 1 2 Kannondai, Tsukuba, Ibaraki 305 8602, Japan
    Plant Mol Biol 65:357-71. 2007
    ..We also show here the other morphological alterations caused by individual FL-cDNA expression. These dominant phenotypes should be valuable indicators for gene discovery and functional analysis...
  76. ncbi Agrobacterium-mediated transformation of embryogenic cultures and plant regeneration in Vitis rotundifolia Michx. (muscadine grape)
    S A Dhekney
    Mid Florida Research and Education Center, University of Florida IFAS, 2725 Binion Road, Apopka, FL 32703 8504, USA
    Plant Cell Rep 27:865-72. 2008
    ..Transgenic plants exhibited uniform GFP expression in cells of all plant tissues and organs including leaves, stems, roots, inflorescences and the embryo and endosperm of developing berries...
  77. ncbi An experimental and modelling exploration of the host-sanction hypothesis in legume-rhizobia mutualism
    Diana E Marco
    Laboratorio de Ecología Matemática, Area de Producción Orgánica, Facultad de Ciencias Agropecuarias, Universidad Nacional de Cordoba, Ciudad Universitaria, CC 509, 5000 Cordoba, Argentina
    J Theor Biol 259:423-33. 2009
    ..against non-N(2) fixing, cheating symbionts have been proposed as a force stabilizing mutualism in legume-Rhizobium symbiosis...
  78. ncbi Rhizobium yanglingense sp. nov., isolated from arid and semi-arid regions in China
    Z Y Tan
    Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing, PR China
    Int J Syst Evol Microbiol 51:909-14. 2001
    ..of 16S rRNA gene sequences showed that isolate CCBAU 71623T, representing cluster 9, was most related to Rhizobium gallicum and Rhizobium mongolense. The DNA-DNA homologies were lower than 42...
  79. ncbi Heme-dependent metalloregulation by the iron response regulator (Irr) protein in Rhizobium and other Alpha-proteobacteria
    Sandra K Small
    Department of Biochemistry and the Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Biometals 22:89-97. 2009
    ..The iron response regulator (Irr) is part of a novel regulatory scheme employed by Rhizobium and other Alpha-Proteobacteria to control iron-dependent gene expression...
  80. ncbi Rhizobium fabae sp. nov., a bacterium that nodulates Vicia faba
    Chang Fu Tian
    Key Laboratory of Agro Microbial Resource and Application, Ministry of Agriculture College of Biological Sciences, China Agricultural University, Beijing, PR China
    Int J Syst Evol Microbiol 58:2871-5. 2008
    ..According to phylogenetic analyses of 16S rRNA gene, atpD and recA sequences, these strains belong to the genus Rhizobium, with Rhizobium etli and Rhizobium leguminosarum as the closest related species...
  81. ncbi Rhizobium miluonense sp. nov., a symbiotic bacterium isolated from Lespedeza root nodules
    Chun Tao Gu
    Key Laboratory of Agro Microbial Resource and Application, Ministry of Agriculture Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, China
    Int J Syst Evol Microbiol 58:1364-8. 2008
    ..Based on data obtained in the present and previous studies, a novel species, Rhizobium miluonense sp. nov., is proposed for these strains. The type strain of R...
  82. ncbi Rhizobium multihospitium sp. nov., isolated from multiple legume species native of Xinjiang, China
    Tian Xu Han
    Key Laboratory of Agro Microbial Resource and Application, Ministry of Agriculture College of Biological Sciences, China Agricultural University, Beijing 100094, PR China
    Int J Syst Evol Microbiol 58:1693-9. 2008
    ..These strains were classified as belonging to the genus Rhizobium based on amplified 16S rDNA restriction analysis (ARDRA)...
  83. ncbi Molecular diversity and phylogeny of rhizobia associated with wild legumes native to Xinjiang, China
    Tian Xu Han
    Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, China
    Syst Appl Microbiol 31:287-301. 2008
    ..Nine genomic species belonging to four genera of Rhizobium, Mesorhizobium, Ensifer, and Bradyrhizobium were defined among these strains based on the characterization of ..
  84. ncbi Rhizobium mesosinicum sp. nov., isolated from root nodules of three different legumes
    Dong Xu Lin
    State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China
    Int J Syst Evol Microbiol 59:1919-23. 2009
    ..Phylogenetic analysis of 16S rRNA gene sequences revealed that they belong to the genus Rhizobium. A representative strain, CCBAU 25010T, showed 98...
  85. ncbi AniA regulates reserve polymer accumulation and global protein expression in Rhizobium etli
    Sergio Encarnación
    Programa de Ingeniería Metabólica, Centro de Investigacion Sobre Fijacion de Nitrogeno, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos CP62210, Mexico
    J Bacteriol 184:2287-95. 2002
    ..capacity and ability to grow on carbon sources such as pyruvate and glucose were severely diminished in the Rhizobium etli phaC::OmegaSm(r)/Sp(r) mutant CAR1, which is unable to synthesize poly-beta-hydroxybutyric acid (PHB) (M. A...
  86. ncbi Nitrate reduction and nitrogen fixation in symbiotic association Rhizobium-legumes
    Robert Lucinski
    Department of Plant Physiology, Adam Mickiewicz University, Poznan, Poland
    Acta Biochim Pol 49:537-46. 2002
    ..Recent advances indicate that symbiotic associations of Rhizobium strains characterized by a high nitrate reductase activity are less susceptible to inhibition by nitrate...
  87. ncbi Evolving insights: symbiosis islands and horizontal gene transfer
    Turlough M Finan
    Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada
    J Bacteriol 184:2855-6. 2002
  88. ncbi Rhizobium mongolense sp. nov. is one of three rhizobial genotypes identified which nodulate and form nitrogen-fixing symbioses with Medicago ruthenica [(L.) Ledebour]
    P van Berkum
    US Department of Agriculture, ARS, Soybean and Alfalfa Research Laboratory, Beltsville, MD 20705, USA
    Int J Syst Bacteriol 48:13-22. 1998
    ..b>Rhizobium tropici also formed nitrogen-fixing symbioses with Medicago ruthenica...
  89. ncbi Rhizobium tibeticum sp. nov., a symbiotic bacterium isolated from Trigonella archiducis-nicolai (Sirj.) Vassilcz
    Bao Chao Hou
    State Key Laboratories for Agro biotechnology College of Biological Sciences, China Agricultural University, 100193 Beijing, PR China
    Int J Syst Evol Microbiol 59:3051-7. 2009
    ..DNA-DNA hybridization, the two strains were very similar and were different from reference strains of defined Rhizobium species...
  90. ncbi Rhizobium sullae sp. nov. (formerly 'Rhizobium hedysari'), the root-nodule microsymbiont of Hedysarum coronarium L
    Andrea Squartini
    Dipartimento di Biotecnologie Agrarie, Universita di Padova, Italy
    Int J Syst Evol Microbiol 52:1267-76. 2002
    ..physiology, genetics and ecology of such organisms, which have in the past been referred to provisionally as 'Rhizobium hedysari'...
  91. ncbi Rhizobium indigoferae sp. nov. and Sinorhizobium kummerowiae sp. nov., respectively isolated from Indigofera spp. and Kummerowia stipulacea
    Ge Hong Wei
    College of Biological Sciences, China Agricultural University, Beijing 100094, People s Republic of China
    Int J Syst Evol Microbiol 52:2231-9. 2002
    ..All the isolates within cluster 1 were isolated from Indigofera and they were identified as Rhizobium strains by 16S rRNA gene analysis. DNA relatedness of 29.5-48...
  92. ncbi Phylogenetic and symbiotic characterization of rhizobial bacteria nodulating Argyrolobium uniflorum in Tunisian arid soils
    M Mahdhi
    Laboratoire de Biotechnologies vegetales Appliquees a l Amelioration des cultures, Faculte des Sciences de Gabes, Cite Erriadh Zrig 6072 Gabes, Tunisia
    Can J Microbiol 54:209-17. 2008
    ..Phylogenetically, the new isolates were grouped in the genera Sinorhizobium (27), Rhizobium (13), and Agrobacterium (2)...
  93. ncbi Agrobacterium-mediated transformation of maize
    Yuji Ishida
    Plant Innovation Center, Japan Tobacco Inc, 700 Higashibara, Iwata, Shizuoka, Japan
    Nat Protoc 2:1614-21. 2007
    ..More than 90% of transformants are expected to be normal in morphology. The protocol takes about 3 months from the start of co-cultivation to the planting of transformants into pots...
  94. ncbi Occurrence of tetraploidy in Nicotiana attenuata plants after Agrobacterium-mediated transformation is genotype specific but independent of polysomaty of explant tissue
    Ben Bubner
    , , 07745, Jena, Germany
    Plant Cell Rep 25:668-75. 2006
    ..We propose that future work on post-transformation polyploidy concentrate on the processes that occur during callus formation and plant regeneration from callus...
  95. ncbi A highly efficient in vitro plant regeneration system and Agrobacterium-mediated transformation in Plumbago zeylanica
    Xiaoping Wei
    Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA
    Plant Cell Rep 25:513-21. 2006
    ..zeylanica cells of petiole, cotyledon and hypocotyl with A. tumefaciens strains AGL1 and LBA4404. Transient expression was observed in all recipient tissues. Stable transgenic calli originating from petiole were obtained...
  96. ncbi Changes in morphological phenotypes and withanolide composition of Ri-transformed roots of Withania somnifera
    Maumita Bandyopadhyay
    Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Calcutta 700019, India
    Plant Cell Rep 26:599-609. 2007
    ..15-0.21% dry weight) and withanolide D (0.08-0.11% dry weight), and they grew faster than the transformed root lines. This is the first report of the presence of withasteroids in undifferentiated callus cultures of W. somnifera...
  97. ncbi A highly efficient transformation protocol for Micro-Tom, a model cultivar for tomato functional genomics
    Hyeon-Jin Sun
    Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan
    Plant Cell Physiol 47:426-31. 2006
    ..Cotyledon explants of tomato inoculated with Agrobacterium tumefaciens (Rhizobium radiobacter) C58C1Rif(R) harboring the binary vector pIG121Hm generated a mass of chimeric non-transgenic and ..
  98. ncbi High efficiency Agrobacterium rhizogenes-mediated transformation of Saponaria vaccaria L. (Caryophyllaceae) using fluorescence selection
    Janice F Schmidt
    Plant Biotechnology Institute, 110 Gymnasium Place, S7N OW9 Saskatoon, SK, Canada
    Plant Cell Rep 26:1547-54. 2007
    ..rhizogenes strain LBA9402 and internode explants precultured on a phytohormone mixture. Selection of cotransformed roots by observation of enhanced green fluorescent protein fluorescence alone was highly effective and convenient...
  99. ncbi The use of green fluorescent protein (GFP) improves Agrobacterium-mediated transformation of 'Spadona' pear (Pyrus communis L.)
    S D Yancheva
    Plant Biotechnology Laboratory, Agricultural University, 12 Mendeleev Street, 4000 Plovdiv, Bulgaria
    Plant Cell Rep 25:183-9. 2006
    ..0-4.0%. We conclude that the introduction of GFP improves the selection of transformed plants of Spadona pear...
  100. ncbi Establishment of Withania somnifera hairy root cultures for the production of withanolide A
    Hosakatte N Murthy
    Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK
    J Integr Plant Biol 50:975-81. 2008
    ..The concentration of withanolide A in transformed roots (157.4 microg/g dry weight) was 2.7-fold more than in non-transformed cultured roots (57.9 microg/g dry weight)...
  101. ncbi Hairy root-activation tagging: a high-throughput system for activation tagging in transformed hairy roots
    Hikaru Seki
    RIKEN Plant Science Center, Kanagawa, Tsurumi-ku, Yokohama 230-0045, Japan
    Plant Mol Biol 59:793-807. 2005
    ..High-throughput metabolic profiling of activation-tagged root culture lines will offer opportunities for identifying regulatory or biosynthetic genes for the production of valuable secondary metabolites of interest...

Research Grants69

  1. RHIZOBIAL LIPOPOLYSACCHARIDES ESSENTIAL FOR INFECTION
    Russell Carlson; Fiscal Year: 2009
    b>Rhizobium-legume symbioses involve processes that are fundamental to all bacterial-host interactions. These include (a.) prokaryotic-eukaryotic recognition, (b.) interaction with the host defense mechanism, and (c...
  2. DnaK Protein Inhibitor Antimicrobials
    Michael Sturgess; Fiscal Year: 2003
    ..abstract_text> ..
  3. STRUCTURES OF ANTIBIOTICS AND RELATED COMPOUNDS
    KENNETH RINEHART; Fiscal Year: 1999
    ..solidum, on hepatotoxins from cyanobacteria, on iron-transport hydroxamates from Hemophilus influenzae, and on a hibernation- and fasting-characteristic compound produced by black bears and humans. ..
  4. LIPID A MODIFICATION SYSTEMS IN GRAM-NEGATIVE BACTERIA
    CHRISTIAN RAETZ; Fiscal Year: 2007
    ..glycolipids that comprise the outer surfaces of Gram-negative bacteria, including the symbiotic organism, Rhizobium leguminosamm...
  5. RHIZOBIAL LIPOPOLYSACCHARIDES ESSENTIAL FOR INFECTION
    Russell Carlson; Fiscal Year: 2007
    b>Rhizobium-legume symbioses involve processes that are fundamental to all bacterial-host interactions. These include (a.) prokaryotic-eukaryotic recognition, (b.) interaction with the host defense mechanism, and (c...
  6. MOLECULAR GENETICS OF RHIZOBIUM NODULATION PLASMIDS
    Graham Walker; Fiscal Year: 2009
    ....
  7. MOLECULAR GENETICS OF RHIZOBIUM NODULATION PLASMIDS
    Graham Walker; Fiscal Year: 2007
    ..abstract_text> ..
  8. MECHANISMS OF POLYSACCHARIDE SYNTHESIS BY S. PNEUMONIAE
    Janet Yother; Fiscal Year: 2005
    ..Homologous proteins (CpsABCD) involved in this type of biosynthesis occur in S. pneumoniae, Rhizobium, other streptococci, and staphylococci. We have demonstrated an interaction among the S...
  9. GENETICS OF DISEASE RESISTANCE IN ARABIDOPSIS THALIANA
    Roger Innes; Fiscal Year: 2003
    ..These experiments will advance understanding of how interactions of plant R genes and pathogen avr genes lead to disease resistance. ..
  10. Genetic analysis of Cryptococcus neoformans virulence
    Joseph Heitman; Fiscal Year: 2007
    ..These studies will enable a genome-wide definition of the gene set contributing to virulence of this common human fungal pathogen. ..
  11. Genetic analysis of Cryptococcus neoformans virulence
    Joseph Heitman; Fiscal Year: 2009
    ..These studies will enable a genome-wide definition of the gene set contributing to virulence of this common human fungal pathogen. ..
  12. INTERACTIONS BETWEEN AGROBACTERIUM AND HOST PLANTS
    STEPHEN WINANS; Fiscal Year: 2007
    ..Direct targets of YenR and YenS will be identified and assessed for their roles in the biology and ecology of Y. enterocolitica and Y. pestis. ..
  13. INTERACTIONS BETWEEN AGROBACTERIUM AND HOST PLANTS
    Stephen C Winans; Fiscal Year: 2010
    ..Direct targets of YenR and YenS will be identified and assessed for their roles in the biology and ecology of Y. enterocolitica and Y. pestis. ..
  14. MOLECULAR GENETICS OF RHIZOBIUM NODULATION PLASMIDS
    Graham C Walker; Fiscal Year: 2010
    ..The project has offered novel insights into the biosynthesis of B12, a critically important vitamin for human health, and will continue to explore the role of vitamin B12 in biology. ..
  15. MOLECULAR GENETICS OF RHIZOBIUM NODULATION PLASMIDS
    Graham Walker; Fiscal Year: 2005
    ..abortus bacA mutants are potential vaccines. Our work will continue to offer insights into how specific low molecular weight oligosaccharides can serve as signals to eukaryotic hosts. ..