Research Topics
Genomes and Genes | zymomonasSummarySummary: A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that is not known to be pathogenic for man, animals, or plants. Its organisms are spoilers for beers and ciders and in sweet English ciders they are the causative agents of a secondary fermentation known as "cider sickness." The species Z. mobilis is used for experiments in molecular genetic studies. Top Publications
Research Grants
| Scientific Experts
|
Detail Information
Publications
Characterization of heterologous and native enzyme activity profiles in metabolically engineered Zymomonas mobilis strains during batch fermentation of glucose and xylose mixturesQiang Gao
Department of Chemical Engineering, University of Colorado, Boulder 80309-0424, USA
Appl Biochem Biotechnol 98:341-55. 2002b>Zymomonas mobilis has been metabolically engineered to broaden its substrate utilization range to include D-xylose and L-arabinose. Both genomically integrated and plasmid-bearing Z...
Cofermentation of glucose, xylose, and arabinose by genomic DNA-integrated xylose/arabinose fermenting strain of Zymomonas mobilis AX101Ali Mohagheghi
Biotechnology Division for Fuels and Chemicals, National Renewable Energy Laboratory, Golden, CO 80401, USA
Appl Biochem Biotechnol 98:885-98. 2002..We have previously developed a plasmid-bearing strain of Zymomonas mobilis (206C[pZB301]) capable of cofermenting glucose, xylose, and arabinose to ethanol...
Performance of immobilized Zymomonas mobilis 31821 (pZB5) on actual hydrolysates produced by Arkenol technologyTomiaki Yamada
JGC, Bio Refinery Group, New Business Development Operations, No 2 Project Division, Yokohama, Japan
Appl Biochem Biotechnol 98:899-907. 2002..in a flash fermentation reactor in which the performance of National Renewable Energy Laboratory's patented rec-Zymomonas mobilis 31821 (pZB5) after immobilization was investigated...
Engineering lactic acid bacteria with pyruvate decarboxylase and alcohol dehydrogenase genes for ethanol production from Zymomonas mobilisNancy N Nichols
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 North University Street, Peoria, IL 61604, USA
J Ind Microbiol Biotechnol 30:315-21. 2003..to express pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH), encoded by the pdc and adhB genes of Zymomonas mobilis, in lactic acid bacteria...
Serine substitution for cysteine residues in levansucrase selectively abolishes levan forming activityVelusamy Senthilkumar
Department of Microbial Technology, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai-625021, India
Biotechnol Lett 25:1653-6. 2003Levansucrase is responsible for levan formation during sucrose fermentation of Zymomonas mobilis, and this decreases the efficiency of ethanol production...
An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xyloseZhanchao Wang
College of Biological Sciences, China Agricultural University, Beijing 100094, P R China
Biotechnol Lett 30:657-63. 2008..Strains JM109 and ET1 were transformed with expression vector pZY507bc containing Zymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes, resulting in an ethanol-..
irrE, an exogenous gene from Deinococcus radiodurans, improves the growth of and ethanol production by a Zymomonas mobilis strain under ethanol and acid stressYing Zhang
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
J Microbiol Biotechnol 20:1156-62. 2010..Here, we present evidence that IrrE acting as a global regulator confers high stress tolerance to a Zymomonas mobilis strain. Expression of the gene protected Z...
Recombinant production of Zymomonas mobilis pyruvate decarboxylase in the haloarchaeon Haloferax volcaniiSteven J Kaczowka
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611-0700, USA
Archaea 1:327-34. 2005..goal of modifying an archaeon to produce bioethanol or other useful products, the pyruvate decarboxylase gene of Zymomonas mobilis (Zm pdc) was expressed in Haloferax volcanii...
Expression, purification and immobilization of the intracellular invertase INVA, from Zymomonas mobilis on crystalline cellulose and Nylon-6María de Los Angeles Calixto-Romo
Departamento de Biotecnologia y Bioingenieria, CINVESTAV IPN Av Instituto Politécnico, Nacional 2508 Col San Pedro Zacatenco, CP 07360, Mexico D F, Mexico
J Ind Microbiol Biotechnol 35:1455-63. 2008This paper presents two immobilization methods for the intracellular invertase (INVA), from Zymomonas mobilis...
Expression of the Escherichia coli pmi gene, encoding phosphomannose-isomerase in Zymomonas mobilis, leads to utilization of mannose as a novel growth substrate, which can be used as a selective markerP Weisser
Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Germany
Appl Environ Microbiol 62:4155-61. 1996Wild-type Zymomonas mobilis can utilize only three substrates (sucrose, glucose, and fructose) as sole carbon sources, which are largely converted into ethanol and carbon dioxide...
Respiratory behaviour of a Zymomonas mobilis adhB::kan(r) mutant supports the hypothesis of two alcohol dehydrogenase isoenzymes catalysing opposite reactionsU Kalnenieks
Institute of Microbiology and Biotechnology, University of Latvia, Kronvalda boulv 4, LV 1586, Riga, Latvia
FEBS Lett 580:5084-8. 2006Perturbation of the aerobic steady-state in a chemostat culture of the ethanol-producing bacterium Zymomonas mobilis with a small pulse of ethanol causes a burst of ethanol oxidation, although the reactant ratio of the alcohol ..
Physiology of Zymomonas mobilis: some unanswered questionsUldis Kalnenieks
Institute of Microbiology and Biotechnology, Chair of Microbiology and Biotechnology, University of Latvia, Kronvalda boulv. 4, Riga, LV-1586, Latvia
Adv Microb Physiol 51:73-117. 2006The ethanol-producing bacterium Zymomonas mobilis can serve as a model organism for the study of rapid catabolism and inefficient energy conversion in bacteria. Some basic aspects of its physiology still remain poorly understood...
Heterologous expression and extracellular secretion of cellulolytic enzymes by Zymomonas mobilisJeffrey G Linger
National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401, USA
Appl Environ Microbiol 76:6360-9. 2010..In this report, the initial steps toward achieving this goal in the fermentation host Zymomonas mobilis were investigated by expressing heterologous cellulases and subsequently examining the potential to ..
NMR analysis of covalent intermediates in thiamin diphosphate enzymesKai Tittmann
Martin Luther Universitat Halle Wittenberg, Institut fur Biochemie, Kurt Mothes Strasse 3, D 06099 Halle, Germany
Biochemistry 42:7885-91. 2003..The application of this method to other ThDP-dependent enzymes provides insight into their specific chemical pathways...
Imidazole acetol phosphate aminotransferase in Zymomonas mobilis: molecular genetic, biochemical, and evolutionary analysesW Gu
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611
J Bacteriol 177:1576-84. 1995hisH encodes imidazole acetol phosphate (IAP) aminotransferase in Zymomonas mobilis and is located immediately upstream of tyrC, a gene which codes for cyclohexadienyl dehydrogenase...
Molecular cloning and characterization of the extracellular sucrase gene (sacC) of Zymomonas mobilisR Kannan
Department of Microbial Technology, School of Biological Sciences, Madurai Kamaraj University, India
Arch Microbiol 163:195-204. 1995The Zymomonas mobilis gene sacC that encodes the extracellular sucrase (protein B46) was cloned and expressed in Escherichia coli...
Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineeringK Deanda
National Renewable Energy Laboratory, Golden, Colorado 80401, USA
Appl Environ Microbiol 62:4465-70. 1996The substrate fermentation range of the ethanologenic bacterium Zymomonas mobilis was expanded to include the pentose sugar, L-arabinose, which is commonly found in agricultural residues and other lignocellulosic biomass...
Kinetic modeling to optimize pentose fermentation in Zymomonas mobilisMehmet M Altintas
Department of Chemical Engineering, University of Colorado, Boulder, Colorado 80309-0424, USA
Biotechnol Bioeng 94:273-95. 2006b>Zymomonas mobilis engineered to express four heterologous enzymes required for xylose utilization ferments xylose along with glucose...
The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4Jeong Sun Seo
Macrogen Inc, World Meridian Venture Center, 60 24, Gasan Dong, Seoul 153 781, Korea
Nat Biotechnol 23:63-8. 2005We report the complete genome sequence of Zymomonas mobilis ZM4 (ATCC31821), an ethanologenic microorganism of interest for the production of fuel ethanol...
Ethanol fermentation on the moveThomas W Jeffries
Nat Biotechnol 23:40-1. 2005
Site-directed mutagenesis of the ionizable groups in the active site of Zymomonas mobilis pyruvate decarboxylase: effect on activity and pH dependenceC Y Huang
Centre for Protein Structure, Function and Engineering, Department of Biochemistry and Molecular Biology, The University of Queensland, Brisbane, Australia
Eur J Biochem 268:3558-65. 2001..45. We conjecture that the kcat/Km profile reflects the protonation of an alcoholate anion intermediate of the catalytic cycle...
Metabolic state of Zymomonas mobilis in glucose-, fructose-, and xylose-fed continuous cultures as analysed by 13C- and 31P-NMR spectroscopyA A de Graaf
Institut für Biotechnologie I, Forschungszentrum Julich, D 52425 Julich, Germany
Arch Microbiol 171:371-85. 1999..for the well-known significantly different behaviour of the anaerobic, gram-negative, ethanologenic bacterium Zymomonas mobilis during growth on fructose (i.e...
Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putidaPetra Siegert
Institute of Molecular Enzyme Technology, , , , Germany
Protein Eng Des Sel 18:345-57. 2005Pyruvate decarboxylase from Zymomonas mobilis (PDC) and benzoylformate decarboxylase from Pseudomonas putida (BFD) are thiamine diphosphate-dependent enzymes that decarboxylate 2-keto acids...
Metabolic engineering of Corynebacterium glutamicum for fuel ethanol production under oxygen-deprivation conditionsMasayuki Inui
Research Institute of Innovative Technology for the Earth (RITE, 9-2 Kizugawadai Kizu, Soraku, Kyoto 619-0292, Japan
J Mol Microbiol Biotechnol 8:243-54. 2004..A recombinant strain which expressed the Zymomonas mobilis genes coding for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) was constructed...
High resolution crystal structure of pyruvate decarboxylase from Zymomonas mobilis. Implications for substrate activation in pyruvate decarboxylasesD Dobritzsch
Department of Medical Biochemistry and Biophysics, Karolinska Institutet S 17177 Stockholm, Sweden
J Biol Chem 273:20196-204. 1998The crystal structure of tetrameric pyruvate decarboxylase from Zymomonas mobilis has been determined at 1.9 A resolution and refined to a crystallographic R-factor of 16.2% and Rfree of 19.7%...
Cofactor activation and substrate binding in pyruvate decarboxylase. Insights into the reaction mechanism from molecular dynamics simulationsMette Alstrup Lie
Danish National Research Foundation, Center for Catalysis
Biochemistry 44:14792-806. 2005We have performed long-term molecular dynamics simulations of pyruvate decarboxylase from Zymomonas mobilis...
Cloning and characterization of a pair of genes that stimulate the production and secretion of Zymomonas mobilis extracellular levansucrase and invertaseY Kondo
Department of Agricultural Chemistry, Faculty of Agriculture, University of Osaka Prefecture, Japan
Biosci Biotechnol Biochem 58:526-30. 1994A 1.7-kb DNA fragment cloned from Zymomonas mobilis genomic DNA complemented the inability to grow on sucrose of a Suc- mutant of Z. mobilis that was deficient in the production of both extracellular levansucrase and invertase...
Zymomonas mobilis for fuel ethanol and higher value productsP L Rogers
School of Biotechnology and Biomolecular Sciences, UNSW, 2052 Sydney, Australia
Adv Biochem Eng Biotechnol 108:263-88. 2007..chemicals, and the recent publication of its genome sequence, have provided continuing stimulus for studies on Zymomonas mobilis...
Metabolic engineering of Escherichia coli for L-tyrosine production by expression of genes coding for the chorismate mutase domain of the native chorismate mutase-prephenate dehydratase and a cyclohexadienyl dehydrogenase from Zymomonas mobilisMaría I Chávez-Béjar
Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Apdo Postal 510 3, Cuernavaca, Morelos 62210, Mexico
Appl Environ Microbiol 74:3284-90. 2008The expression of the feedback inhibition-insensitive enzyme cyclohexadienyl dehydrogenase (TyrC) from Zymomonas mobilis and the chorismate mutase domain from native chorismate mutase-prephenate dehydratase (PheA(CM)) from Escherichia ..
Identification of functionally important amino acid residues in Zymomonas mobilis levansucraseHideshi Yanase
Department of Biotechnology, Tottori University, Koyamacho minami, Tottori, Tottori 680 8552, Japan
J Biochem 132:565-72. 2002..4.1.10) from Zymomonas mobilis were analyzed by random mutation and site-directed mutagenesis...
Analysis of the respiratory chain in Ethanologenic Zymomonas mobilis with a cyanide-resistant bd-type ubiquinol oxidase as the only terminal oxidase and its possible physiological rolesKaewta Sootsuwan
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan
J Mol Microbiol Biotechnol 14:163-75. 2008The respiratory chain of the ethanologenic bacterium Zymomonas mobilis was investigated, in which the pyruvate-to-ethanol pathway has been demonstrated to be mainly responsible for NADH oxidation and the tricarboxylic acid cycle is ..
Sequence analysis and overexpression of the Zymomonas mobilis tgt gene encoding tRNA-guanine transglycosylase: purification and biochemical characterization of the enzymeK Reuter
Institut fur Biochemie, Universitat Erlangen Nurnberg, Germany
J Bacteriol 177:5284-8. 1995..A partially sequenced gene upstream of the DNA ligase (lig) gene of the Zymomonas mobilis chromosome shows strong homology to the tgt gene of Escherichia coli (K.B. Shark and T...
Performance testing of Zymomonas mobilis metabolically engineered for cofermentation of glucose, xylose, and arabinoseHugh G Lawford
Bio Engineering Laboratory, Department of Biochemistry, University of Toronto, Ontario, Canada
Appl Biochem Biotechnol 98:429-48. 2002..performance characteristics of the National Renewable Energy Labora-tory's metabolically engineered Zymomonas mobilis using various biomass hydrolysates...
NADH dehydrogenase deficiency results in low respiration rate and improved aerobic growth of Zymomonas mobilisUldis Kalnenieks
Institute of Microbiology and Biotechnology, University of Latvia, Kronvalda boulv 4, LV 1586, Riga, Latvia
Microbiology 154:989-94. 2008The respiratory chain of the ethanol-producing bacterium Zymomonas mobilis is able to oxidize both species of nicotinamide cofactors, NADH and NADPH...
Immobilization of the recombinant invertase INVB from Zymomonas mobilis on Nylon-6Vanessa Vallejo-Becerra
Departamento de Biotecnologia y Bioingenieria, CINVESTAV, IPN Av Instituto Politécnico Nacional 2508, Col San Pedro Zacatenco, 07360, Mexico D F, Mexico
J Ind Microbiol Biotechnol 35:1289-95. 2008The recombinant invertase INVB (re-INVB) from Zymomonas mobilis was immobilized on microbeads of Nylon-6, by means of covalent bonding. The enzyme was strongly and successfully bound to the support...
Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentationsShihui Yang
Biosciences Division and BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
BMC Genomics 10:34. 2009b>Zymomonas mobilis ZM4 (ZM4) produces near theoretical yields of ethanol with high specific productivity and recombinant strains are able to ferment both C-5 and C-6 sugars. Z...
Molecular characterization of the Zymomonas mobilis enolase (eno) geneM E Burnett
School of Biological Sciences, University of Nebraska, Lincoln 68588 0118
J Bacteriol 174:6548-53. 1992The Zymomonas mobilis gene encoding enolase was cloned by genetic complementation of an Escherichia coli eno mutant...
The glutamate uptake regulatory protein (Grp) of Zymomonas mobilis and its relation to the global regulator Lrp of Escherichia coliN Peekhaus
Institut für Biotechnologie I, Forschungszentrum Julich, Germany
J Bacteriol 177:5140-7. 1995After being expressed in Escherichia coli JC5412, which is defective in glutamate transport, a Zymomonas mobilis gene which enabled this strain to grow on glutamate was cloned...
Inhibition of thiamin diphosphate dependent enzymes by 3-deazathiamin diphosphateStephane Mann
University Chemical Laboratory, Lensfield Road, Cambridge, UKCB2 1EW
Org Biomol Chem 2:1732-41. 2004..These compounds are both extremely potent inhibitors of pyruvate decarboxylase from Zymomonas mobilis; binding is competitive with TPP and is essentially irreversible even though no covalent linkage is ..
Isolation of the dxr gene of Zymomonas mobilis and characterization of the 1-deoxy-D-xylulose 5-phosphate reductoisomeraseS Grolle
Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, 52425, Julich, Germany
FEMS Microbiol Lett 191:131-7. 2000..diphosphate biosynthesis, 1-deoxy-D-xylulose 5-phosphate (DXP) reductoisomerase, was cloned and sequenced from Zymomonas mobilis. The deduced amino acid sequence showed the highest identity (48...
Performance of a newly developed integrant of Zymomonas mobilis for ethanol production on corn stover hydrolysateAli Mohagheghi
National Bioenergy Center National Renewable Energy Laboratory, Golden, CO 80401, USA
Biotechnol Lett 26:321-5. 2004..Recombinant Zymomonas mobilis 8b, a recently developed integrant of Zymomonas mobilis 31821(pZB5), tolerated acetic acid up to 16 g l(-..
An in vivo, label-free quick assay for xylose transport in Escherichia coliTingjian Chen
Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
Anal Biochem 390:63-7. 2009..XynB was expressed alone or in conjunction with a Zymomonas mobilis glucose facilitator protein (Glf) capable of transporting xylose...
Asymmetric alkene reduction by yeast old yellow enzymes and by a novel Zymomonas mobilis reductaseAndre Muller
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia
Biotechnol Bioeng 98:22-9. 2007..encoding yeast old yellow enzymes (OYE 1, 2, and 3) and NAD(P)H-dependent 2-cyclohexen-1-one reductase from Zymomonas mobilis (NCR) were expressed separately in Escherichia coli...
An allosterically insensitive class of cyclohexadienyl dehydrogenase from Zymomonas mobilisG Zhao
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611 0100
Eur J Biochem 212:157-65. 1993The key enzyme of tyrosine biosynthesis in many Gram-negative prokaryotes is cyclohexadienyl dehydrogenase. The Zymomonas mobilis gene (tyrC) coding for this enzyme was cloned in Escherichia coli by complementation of a tyrosine ..
Detection of aromatic alpha-hydroxyketones with tetrazolium saltsMichael Breuer
BASF-AG, Fine Chemicals and Biocatalysis Research, Ludwigshafen, Germany
Methods Mol Biol 230:223-9. 2003
Catalytic activity of zymomonas mobilis extracellular "levan-levansucrase" complex in sucrose mediumM Bekers
Institute of Microbiology and Biotechnology, University of Latvia, Kronvalda boulevard 4, LV 1586, Riga, Latvia
Commun Agric Appl Biol Sci 68:321-4. 2003The fructan biosynthesis by ethanol sedimented "levan-levansucrase" complex from Zymomonas mobilis fermentation broth as well as purified levansucrase was investigated...
Structure and properties of pyruvate decarboxylase and site-directed mutagenesis of the Zymomonas mobilis enzymeJ M Candy
Centre for Protein Structure, Function and Engineering, Department of Biochemistry, University of Queensland, Brisbane 4072, Australia
Biochim Biophys Acta 1385:323-38. 1998..Here we review its structure and properties with particular emphasis on how site-directed mutagenesis of the enzyme from Zymomonas mobilis has assisted us to understand the function of critical residues.
Cellulosic fuel ethanol: alternative fermentation process designs with wild-type and recombinant Zymomonas mobilisHugh G Lawford
Bio Engineering Laboratory, Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8
Appl Biochem Biotechnol 105:457-69. 2003..with the University of Toronto to test the fermentation performance characteristics of metabolically engineered Zymomonas mobilis created at the National Renewable Energy Laboratory...
The biotechnological production of sorbitolM M Silveira
Universidade de Caxias do Sul-Instituto de Biotecnologia. CP 1352, 95001-970 Caxias do Sul, RS, Brazil
Appl Microbiol Biotechnol 59:400-8. 2002..At present, it is produced chemically. The bacterium Zymomonas mobilis is able to produce sorbitol and gluconic acid from fructose and glucose, respectively...
Altered glucose transport and shikimate pathway product yields in E. coliJian Yi
Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA
Biotechnol Prog 19:1450-9. 2003..Use of heterologously expressed Zymomonas mobilis glf-encoded glucose facilitator and glk-encoded glucokinase resulted in the synthesis in 48 h of 3-..
A novel and simple method for the purification of extracellular levansucrase from Zymomonas mobilisArmands Vigants
Institute of Microbiology and Biotechnology, University of Latvia, Kronvalda boulevard 4, 1586 Riga, Latvia
Curr Microbiol 47:198-202. 2003A new and simple method for the purification of extracellular levansucrase from Zymomonas mobilis from highly viscous fermentation broth was developed...
Ethanol cycle in an ethanologenic bacteriumU Kalnenieks
Institute of Microbiology and Biotechnology, University of Latvia, Kronvalda boulv 4, LV 1586, Riga, Latvia
FEBS Lett 522:6-8. 2002..performing interconversion between acetaldehyde and ethanol in aerobically growing ethanologenic bacterium Zymomonas mobilis...
High-level acetaldehyde production in Lactococcus lactis by metabolic engineeringRoger S Bongers
NIZO Food Research, Department of Flavour and Natural Ingredients, P O Box 20, 6710 BA Ede, The Netherlands
Appl Environ Microbiol 71:1109-13. 2005Efficient conversion of glucose to acetaldehyde is achieved by nisin-controlled overexpression of Zymomonas mobilis pyruvate decarboxylase (pdc) and Lactococcus lactis NADH oxidase (nox) in L. lactis...
High-solid enzymatic hydrolysis and fermentation of solka floc into ethanolByung Hwan Um
Forest Bioproducts Research Initiative, Department of Chemical Engineering, University of Maine, Orono, Maine 04469, USA
J Microbiol Biotechnol 18:1257-65. 2008..In this process, commercial enzymes were used in combination with a recombinant strain of Zymomonas mobilis (39679:pZB4L)...
A comparison of simple rheological parameters and simulation data for Zymomonas mobilis fermentation broths with high substrate loading in a 3-L bioreactorByung Hwan Um
Department of Chemical Engineering, Samuel Ginn College of Engineering, Auburn University, Auburn, AL 36849, USA
Appl Biochem Biotechnol 145:29-38. 2008..Computational fluid dynamics analysis of bioreactor mixing demonstrates the change in bioreactor mixing with increasing biomass concentration. The portion-loading method is shown to be effective for processing high-solids slurries...
Heterologous expression of pyruvate decarboxylase in Geobacillus thermoglucosidasiusAnn H Thompson
Division of Biology, Faculty of Natural Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK
Biotechnol Lett 30:1359-65. 2008..We have demonstrated that Pdc from Zymomonas mobilis can be expressed in an active form in Geobacillus thermoglucosidasius at up to 52 degrees C, while ..
Fermentable metabolite of Zymomonas mobilis controls collagen reduction in photoaging skin by improving TGF-beta/Smad signaling suppressionHiroshi Tanaka
Research Laboratories, Nippon Menard Cosmetic Co Ltd, 2 7 Torimi cho, Nishi ku, Nagoya 451 0071, Japan
Arch Dermatol Res 300:S57-64. 2008..b>Zymomonas mobilis is an alcohol fermentable, gram-negative facultative anaerobic bacterium whose effect on skin tissue is ..
Improvement of P450(BM-3) whole-cell biocatalysis by integrating heterologous cofactor regeneration combining glucose facilitator and dehydrogenase in E. coliHendrik Schewe
Biochemical Engineering Group, DECHEMA e V, Karl Winnacker Institut, Theodor Heuss Allee 25, 60486, Frankfurt, Germany
Appl Microbiol Biotechnol 78:55-65. 2008..a recombinant intracellular NADPH regeneration system through co-expression of a glucose facilitator from Zymomonas mobilis for uptake of unphosphorylated glucose and a NADP(+)-dependent glucose dehydrogenase from Bacillus ..
Zymomonas mobilis as catalyst for the biotechnological production of sorbitol and gluconic acidGilmar Sidney Erzinger
Pharmacy School, University of Joinville, Santa Catarina, SC, Brazil
Appl Biochem Biotechnol 131:787-94. 2006..GA) and sorbitol (SOR) was conducted in a batch reactor with free (CTAB-treated or not) or immobilized cells of Zymomonas mobilis...
Ethanol production from hydrolysed agricultural wastes using mixed culture of Zymomonas mobilis and Candida tropicalisSonali Patle
TERI University, Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi, 110003, India
Biotechnol Lett 29:1839-43. 2007..of total reducing sugars with the hydrolysates being evaluated for ethanol production using a mixed culture of Zymomonas mobilis and Candida tropicalis...
Fermentation of molasses by Zymomonas mobilis: effects of temperature and sugar concentration on ethanol productionM L Cazetta
Department of Food and Drugs, Londrina State University, Post Box 6001, CEP 86051 990, Londrina PR, Brazil
Bioresour Technol 98:2824-8. 2007Fermentations utilizing strains of Zymomonas mobilis, in place of the traditional yeasts, have been proposed due their ethanol yields being close to theoretical...
Genetic engineering of Zymobacter palmae for production of ethanol from xyloseHideshi Yanase
Department of Biotechnology, Faculty of Engineering, Tottori University, 4 101 Koyamacho minami, Tottori, Tottori, Japan
Appl Environ Microbiol 73:2592-9. 2007..palmae, where their expression was driven by the Zymomonas mobilis glyceraldehyde-3-phosphate dehydrogenase promoter...
Zymomonas mobilis as catalyst for the biotechnological production of sorbitol and gluconic acidGilmar Sidney Erzinger
University of Joinville, Pharmacy School, Santa Catarina, SC, Brazil
Appl Biochem Biotechnol 129:787-94. 2006..GA) and sorbitol (SOR) was conducted in a batch reactor with free (CTAB-treated or not) or immobilized cells of Zymomonas mobilis...
Measurement and analysis of intracellular ATP levels in metabolically engineered Zymomonas mobilis fermenting glucose and xylose mixturesJuan Carlos Saez-Miranda
Department of Chemical Engineering, University of Puerto Rico, Mayaguez Campus, P O Box 9046 Mayagüez, Puerto Rico 00681 9046
Biotechnol Prog 22:359-68. 2006Intracellular adenosine-5'-triphosphate (ATP) levels were measured in a metabolically engineered Zymomonas mobilis over the course of batch fermentations of glucose and xylose mixtures...
Enhanced benzaldehyde tolerance in Zymomonas mobilis biofilms and the potential of biofilm applications in fine-chemical productionXuan Zhong Li
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia
Appl Environ Microbiol 72:1639-44. 2006..b>Zymomonas mobilis was used in this study as a model organism to examine the potential of surface-associated biofilms for ..
High efficiency ethanol fermentation by entrapment of Zymomonas mobilis into LentiKatsM Rebros
Faculty of Chemical and Food Technology, Department of Biotechnology, Slovak Technical University, Slovakia
Lett Appl Microbiol 41:412-6. 2005To examine the potential of Zymomonas mobilis entrapped into polyvinylalcohol (PVA) lens-shaped immobilizates in batch and continuous ethanol production.
The role of aspartic acid 143 in E. coli tRNA-guanine transglycosylase: insights from mutagenesis studies and computational modelingKatherine Abold Todorov
Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109 1065, USA
Biophys J 89:1965-77. 2005..The crystal structure of Zymomonas mobilis TGT with preQ(1) bound suggests that a key aspartate is critically involved in substrate recognition...
Solvent kinetic isotope effects monitor changes in hydrogen bonding at the active center of yeast pyruvate decarboxylase concomitant with substrate activation: the substituent at position 221 can control the state of activationWen Wei
Department of Chemistry and Program in Cellular and Molecular Biodynamics, Rutgers, The State University of New Jersey, Newark, New Jersey 07102, USA
Biochemistry 41:451-61. 2002..A comparison with SKIE for the wild-type enzyme provides insight to changes in hydrogen bonding at the active center as a result of substrate activation...
Construction of a novel cell-surface display system for heterologous gene expression in Escherichia coli by using an outer membrane protein of Zymomonas mobilis as anchor motifMing Xiong He
Sichuan Key Laboratory of Molecular Biology and Biotechnology, Key Laboratory of Resource Biology and Eco environment of Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China
Biotechnol Lett 30:2111-7. 2008..display system was developed in Escherichia coli using omp1, a hypothetical outer membrane protein of Zymomonas mobilis. By using this system, we successfully expressed beta-amylase gene of sweet potato in E. coli...
Improvement of cellulolytic properties of Clostridium cellulolyticum by metabolic engineeringEmmanuel Guedon
, , , , France
Appl Environ Microbiol 68:53-8. 2002..accumulation is decreased by heterologous expression of pyruvate decarboxylase and alcohol dehydrogenase from Zymomonas mobilis...
The role of residues glutamate-50 and phenylalanine-496 in Zymomonas mobilis pyruvate decarboxylaseJ M Candy
Department of Biochemistry, University of Queensland, Brisbane, Australia
Biochem J 315:745-51. 1996..1.1.1) from Zymomonas mobilis, as a model for this group of enzymes...
Cloning, sequencing and expression of stress genes from the ethanol-producing bacterium Zymomonas mobilis: the groESL operonM F Barbosa
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611
Gene 148:51-7. 1994b>Zymomonas mobilis is unique among bacteria in its ability to produce high levels of ethanol (EtOH) during fermentation...
Enzymes of the Entner-Doudoroff and pyruvate decarboxylation pathways in Zymomonas mobilis wild-type CP4 and mutant strains grown in continuous cultureA L Savvides
Department of Botany, Faculty of Biology, University of Athens, Greece
Antonie Van Leeuwenhoek 80:287-95. 2001The osmotolerant Zymomonas mobilis strain suc40, (containing plasmid pDS3154-inaZ), which is capable of producing simultaneously ethanol and ice nuclei protein, was cultivated in a chemically defined complete sucrose medium, as well as ..
Purification of food-grade oligosaccharides using immobilised cells of Zymomonas mobilisR G Crittenden
Food Science Australia, Victoria
Appl Microbiol Biotechnol 58:297-302. 2002Immobilised cells of the bacterium Zymomonas mobilis were used to remove glucose, fructose, and sucrose from food-grade oligosaccharide mixtures...
Continuous production of (R)-phenylacetylcarbinol in an enzyme-membrane reactor using a potent mutant of pyruvate decarboxylase from Zymomonas mobilisG Goetz
, , Germany
Biotechnol Bioeng 74:317-25. 2001..We compare the suitability of three pyruvate decarboxylases (PDC), PDC from Saccharomyces cerevisiae, PDC from Zymomonas mobilis, and a potent mutant of the latter, PDCW392M, with respect to their application in the biotransformation ..
Steady-state measurements of lactic acid production in a wild-type and a putative D-lactic acid dehydrogenase-negative mutant of Zymomonas mobilis: influence of glycolytic fluxHugh G Lawford
Department of Biochemistry, University of Toronto, Ontario, Canada
Appl Biochem Biotechnol 98:215-28. 2002..a continuation of our investigation into environmental conditions that promote lactic acid synthesis by Zymomonas mobilis. The characteristic near theoretical yield of ethanol from glucose by Z...
Modeling and advanced control of recombinant Zymomonas mobilis fed-batch fermentationDavid B Hodge
Department of Chemical and Bioresource Engineering, Colorado State University, Ft Collins, Colorado 80523, USA
Biotechnol Prog 18:572-9. 2002..degrees of product, substrate, and pH inhibition on the kinetic rates of ethanol fermentation by recombinant Zymomonas mobilis CP4:pZB5 for growth on two substrates...
Evaluation of a recombinant Klebsiella oxytoca strain for ethanol production from cellulose by simultaneous saccharification and fermentation: comparison with native cellobiose-utilising yeast strains and performance in co-culture with thermotolerant yeasHelen Golias
Department of Chemical Engineering, University of Melbourne, Parkville, Victoria, Australia
J Biotechnol 96:155-68. 2002..investigated, including ethanol-tolerant strains of Saccharomyces pastorianus, Kluyveromyces marxianus and Zymomonas mobilis...
Disruption of the Zymomonas mobilis extracellular sucrase gene (sacC) improves levan productionV Senthilkumar
Department of Microbial Technology, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, India
J Appl Microbiol 96:671-6. 2004AIMS: Disruption of the extracellular Zymomonas mobilis sucrase gene (sacC) to improve levan production. METHODS AND RESULTS: A PCR-amplified tetracycline resistance cassette was inserted within the cloned sacC gene in pZS2811...
Specific spoilage organisms in breweries and laboratory media for their detectionL Jespersen
Royal Veterinary and Agricultural University, Department of Dairy and Food Science, Food Microbiology, Frederiksberg, Denmark
Int J Food Microbiol 33:139-55. 1996..beer spoilage organism; Megasphaera cerevisiae as an obligate spoilage organism of low alcohol beer and Zymomonas mobilis as capable of spoiling primed beer...
Analysis of bacterial community during the fermentation of pulque, a traditional Mexican alcoholic beverage, using a polyphasic approachAdelfo Escalante
Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico UNAM, Avenida Universidad 2001, Col Chamilpa, Cuernavaca, Morelos, Mexico
Int J Food Microbiol 124:126-34. 2008..homofermentative lactobacilli related to Lactobacillus acidophilus, several alpha-Proteobacteria such as Zymomonas mobilis and Acetobacter malorum, other gamma-Proteobacteria and an important amount of yeasts, creating a ..
Identification and characterization of phoN-Sf, a gene on the large plasmid of Shigella flexneri 2a encoding a nonspecific phosphataseK I Uchiya
Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan
J Bacteriol 178:4548-54. 1996..2%), Morganella morganii (PhoC, 80.6%), Salmonella typhimurium (PhoN, 47.8%), and Zymomonas mobilis (PhoC, 34.8%). The PhoN-Sf protein was purified, and its biochemical properties were characterized...
Ethanol production from cellobiose, amorphous cellulose, and crystalline cellulose by recombinant Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes frB E Wood
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611
Appl Environ Microbiol 58:2103-10. 1992The Zymomonas mobilis genes for ethanol production have been integrated into the chromosome of Klebsiella oxytoca M5A1. The best of these constructs, strain P2, produced ethanol efficiently from cellobiose in addition to monomeric sugars...
Crystal structure of histidinol phosphate aminotransferase (HisC) from Escherichia coli, and its covalent complex with pyridoxal-5'-phosphate and l-histidinol phosphateJ Sivaraman
Biotechnology Research Institute, 6100 Royalmount Ave, Montreal, H4P 2R2 and, Canada
J Mol Biol 311:761-76. 2001..bond with Tyr110, a residue that is substituted by Phe in the broad substrate specific HisC enzymes from Zymomonas mobilis and Bacillus subtilis...
Tuber-specific expression of a yeast invertase and a bacterial glucokinase in potato leads to an activation of sucrose phosphate synthase and the creation of a sucrose futile cycleR N Trethewey
, Karl Liebknecht Str 25, D 14476 Golm, Germany
Planta 208:227-38. 1999..2.1.26;U-IN2-30) or in combination with a Zymomonas mobilis glucokinase (EC 2.7.1...
Isolation and sequence analysis of rpoH genes encoding sigma 32 homologs from gram negative bacteria: conserved mRNA and protein segments for heat shock regulationK Nakahigashi
HSP Research Institute, Kyoto Research Park, Japan
Nucleic Acids Res 23:4383-90. 1995..cloacae (gamma), Serratia marcescens (gamma), Proteus mirabilis (gamma), Agrobacterium tumefaciens (alpha) and Zymomonas mobilis (alpha). Comparison of these and three known genes from E...
Adaptation yields a highly efficient xylose-fermenting Zymomonas mobilis strainManoj Agrawal
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, Georgia 30332 0100, USA
Biotechnol Bioeng 108:777-85. 2011b>Zymomonas mobilis is a superb ethanol producer with productivity exceeding yeast strains by several fold...
Extension of the substrate utilization range of Ralstonia eutropha strain H16 by metabolic engineering to include mannose and glucoseShanna Sichwart
Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat Munster, Corrensstrasse 3, D 48149 Munster, Germany
Appl Environ Microbiol 77:1325-34. 2011..The genes for a facilitated diffusion protein (glf) from Zymomonas mobilis and for a glucokinase (glk), mannofructokinase (mak), and phosphomannose isomerase (pmi) from ..
Squalene-hopene cyclase from Bradyrhizobium japonicum: cloning, expression, sequence analysis and comparison to other triterpenoid cyclasesM Perzl
Universitat Tubingen, Germany
Microbiology 143:1235-42. 1997..sequence with the sequences of other SHCs revealed highest similarity (70%) to the SHC from the Gram-negative Zymomonas mobilis and lower similarity (48%) to the SHCs from the Gram-positive Alicyclobacillus acidocaldarius and ..
Effect of acetic acid on xylose conversion to ethanol by genetically engineered E. coliH G Lawford
Department of Biochemistry, University of Toronto, Ontario, Canada
Appl Biochem Biotechnol 34:185-204. 1992..B (ATCC 11303), carrying the plasmid pLO1297 with pyruvate decarboxylase and alcohol dehydrogenase II genes from Zymomonas mobilis (CP4), converts xylose to ethanol with a product yield that approaches theoretical maximum...
Myo-inositol facilitators IolT1 and IolT2 enhance D-mannitol formation from D-fructose in Corynebacterium glutamicumCarsten Bäumchen
Institut fur Biotechnologie, Forschungszentrum Julich GmbH, Julich, Germany
FEMS Microbiol Lett 290:227-35. 2009..uptake of d-fructose in its unphosphorylated form, because additional expression of the sugar facilitator from Zymomonas mobilis resulted in a significantly increased productivity...
Novel xylose dehydrogenase in the halophilic archaeon Haloarcula marismortuiUlrike Johnsen
Institut fur Allgemeine Mikrobiologie, Christian Albrechts Universitat Kiel, Am Botanischen Garten 1 9, D 24118 Kiel, Germany
J Bacteriol 186:6198-207. 2004..Xylose dehydrogenase showed the highest sequence similarity to glucose-fructose oxidoreductase from Zymomonas mobilis and other putative bacterial and archaeal oxidoreductases...
Growth and metabolism in sugarcane are altered by the creation of a new hexose-phosphate sinkBarrie Fong Chong
David North Plant Research Centre, BSES Limited, PO Box 86, Indooroopilly, Qld 4068, Australia
Plant Biotechnol J 5:240-53. 2007..When the Zymomonas mobilis glucokinase (zmglk) gene was co-expressed with mds6pdh to increase the production of glucose-6-phosphate,..
Construction and characterization of an effector strain of Streptococcus mutans for replacement therapy of dental cariesJ D Hillman
Department of Oral Biology, University of Florida College of Dentistry, Gainesville, Florida 32610, USA
Infect Immun 68:543-9. 2000..imbalance, a supplemental alcohol dehydrogenase activity was introduced by substituting the adhB ORF from Zymomonas mobilis in place of the deleted ldh ORF...
Phosphoenolpyruvate availability and the biosynthesis of shikimic acidSunil S Chandran
Department of Chemistry, Michigan State University, East Lansing, Michigan 48824 1322, USA
Biotechnol Prog 19:808-14. 2003..coli ppsA-encoded phosphoenolpyruvate synthase or heterologous expression of the Zymomonas mobilis glf-encoded glucose facilitator...
Molecular characterization of the levansucrase gene from the endophytic sugarcane bacterium Acetobacter diazotrophicus SRT4J Arrieta
Center for Genetic Engineering and Biotechnology, Agriculture Division, Havana, Cuba
Microbiology 142:1077-85. 1996..acid sequence of the IsdA gene was 48% and 46% identical with the levansucrases from the Gram-negative bacteria Zymomonas mobilis and Erwinia amylovora, respectively, but only 28-31% identical with levansucrases from Gram-positive ..
Metabolic engineering of Bacillus subtilis for ethanol production: lactate dehydrogenase plays a key role in fermentative metabolismSusana Romero
Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, A P 510 3 Cuernavaca, MOR 62250, Mexico
Appl Environ Microbiol 73:5190-8. 2007..was used to disrupt the native lactate dehydrogenase (LDH) gene (ldh) by chromosomal insertion of the Zymomonas mobilis pyruvate decarboxylase gene (pdc) and alcohol dehydrogenase II gene (adhB) under the control of the ldh ..
Symbiotic induction of pyruvate dehydrogenase genes from Sinorhizobium melilotiD Cabanes
Laboratoire de Biologie Moleculaire des relations Plantes Microorganismes, CNRS INRA, Castanet Tolosan, France
Mol Plant Microbe Interact 13:483-93. 2000..bp) and pdhAbeta (1,383 bp), a situation encountered in the alpha-proteobacteria Rickettsia prowazekii and Zymomonas mobilis as well as in some gram-positive bacteria and in mitochondria...
Proposal of Sphingomonadaceae fam. nov., consisting of Sphingomonas Yabuuchi et al. 1990, Erythrobacter Shiba and Shimidu 1982, Erythromicrobium Yurkov et al. 1994, Porphyrobacter Fuerst et al. 1993, Zymomonas Kluyver and van Niel 1936, and SandaracinobacY Kosako
Japan Collection of Microorganisms, RIKEN Institute of Physical and Chemical Research, Wako, Saitama
Microbiol Immunol 44:563-75. 2000..dihydrosphingosine 1-glucuronic acid (SGL-1) and 2-hydroxymyristic acid (non-hydroxymyristic acid in Zymomonas) in cellular lipids, a new family, Sphingomonadaceae, for Group 4 of the alpha-subclass of the class ..
The Zymomonas mobilis glf, zwf, edd, and glk genes form an operon: localization of the promoter and identification of a conserved sequence in the regulatory regionW O Barnell
School of Biological Sciences, University of Nebraska, Lincoln 68588 0118
J Bacteriol 174:2816-23. 1992The Zymomonas mobilis genes that encode the glucose-facilitated diffusion transporter (glf), glucose-6-phosphate dehydrogenase (zwf), 6-phosphogluconate dehydratase (edd), and glucokinase (glk) are clustered on the genome...
TFAM detects co-evolution of tRNA identity rules with lateral transfer of histidyl-tRNA synthetaseDavid H Ardell
Department of Molecular Evolution, Evolutionary Biology Center Norbyvägen 18C Uppsala University SE 752 36 Uppsala Sweden
Nucleic Acids Res 34:893-904. 2006..Caulobacter crescentus and Silicibacter pomeroyi, unlike a sister clade containing the Rickettsiales, Zymomonas mobilis and Gluconobacter oxydans, uses the eukaryotic identity element A73 instead of the highly conserved ..
Research Grants
- Phenotype MicroArrays for Drug Toxicity ScreeningBarry Bochner; Fiscal Year: 2003..In future studies (Phase II SBIR) we will expand the technology and scope of reference drugs tested to build a strong predictive database including cell lines representing more tissues and organs. ..
- Detection of Bioterrorism Agents with PM TechnologyBarry Bochner; Fiscal Year: 2006..abstract_text> ..
- Phenotype MicroArrays for Drug Toxicity ScreeningBarry Bochner; Fiscal Year: 2007....
- Phenotype MicroArray Analysis of Fastidious PathogensBarry Bochner; Fiscal Year: 2007..It will be applied in hospital diagnostic work, environmental diagnostic work, bioterrorism monitoring, new drug development, toxicology studies, and in many areas of basic biology research. ..
