enterococcus faecium

Summary

Summary: A species of gram-positive, coccoid bacteria whose organisms are normal flora of the intestinal tract. Unlike ENTEROCOCCUS FAECALIS, this species may produce an alpha-hemolytic reaction on blood agar and is unable to utilize pyruvic acid as an energy source.

Top Publications

  1. ncbi Novel mechanism of beta-lactam resistance due to bypass of DD-transpeptidation in Enterococcus faecium
    J L Mainardi
    L R M A, UFR Broussais Hotel Dieu, Universite Paris VI, 75270 Paris, France
    J Biol Chem 275:16490-6. 2000
  2. pmc Genetic basis for daptomycin resistance in enterococci
    Kelli L Palmer
    Departments of Ophthalmology and Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, USA
    Antimicrob Agents Chemother 55:3345-56. 2011
  3. pmc Pyrosequencing-based comparative genome analysis of the nosocomial pathogen Enterococcus faecium and identification of a large transferable pathogenicity island
    Willem van Schaik
    Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    BMC Genomics 11:239. 2010
  4. doi Mobile genetic elements and their contribution to the emergence of antimicrobial resistant Enterococcus faecalis and Enterococcus faecium
    K Hegstad
    Reference Centre for Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway
    Clin Microbiol Infect 16:541-54. 2010
  5. pmc Distribution of genes encoding MSCRAMMs and Pili in clinical and natural populations of Enterococcus faecium
    Jouko Sillanpaa
    Department of Internal Medicine, Division of Infectious Diseases, University of Texas Medical School at Houston, Houston, TX 77030, USA
    J Clin Microbiol 47:896-901. 2009
  6. doi Transition of Enterococcus faecium from commensal organism to nosocomial pathogen
    Rob Jl Willems
    Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan, CX Utrecht, The Netherlands
    Future Microbiol 4:1125-35. 2009
  7. doi A genetic element present on megaplasmids allows Enterococcus faecium to use raffinose as carbon source
    Xinglin Zhang
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    Environ Microbiol 13:518-28. 2011
  8. pmc Restricted gene flow among hospital subpopulations of Enterococcus faecium
    Rob J L Willems
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    MBio 3:e00151-12. 2012
  9. pmc Management of multidrug-resistant enterococcal infections
    C A Arias
    Department of Internal Medicine, Division of Infectious Diseases and Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School at Houston, Houston, TX, USA
    Clin Microbiol Infect 16:555-62. 2010
  10. pmc Genome-wide identification of ampicillin resistance determinants in Enterococcus faecium
    Xinglin Zhang
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS Genet 8:e1002804. 2012

Research Grants

Detail Information

Publications281 found, 100 shown here

  1. ncbi Novel mechanism of beta-lactam resistance due to bypass of DD-transpeptidation in Enterococcus faecium
    J L Mainardi
    L R M A, UFR Broussais Hotel Dieu, Universite Paris VI, 75270 Paris, France
    J Biol Chem 275:16490-6. 2000
    The peptidoglycan structure of in vitro selected ampicillin-resistant mutant Enterococcus faecium D344M512 and of the susceptible parental strain D344S was determined by reverse phase high performance liquid chromatography and mass ..
  2. pmc Genetic basis for daptomycin resistance in enterococci
    Kelli L Palmer
    Departments of Ophthalmology and Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, USA
    Antimicrob Agents Chemother 55:3345-56. 2011
    ..Since its introduction, resistance to daptomycin by strains of Enterococcus faecalis and Enterococcus faecium has been reported but is still rare. We evolved daptomycin-resistant strains of the multidrug-resistant E...
  3. pmc Pyrosequencing-based comparative genome analysis of the nosocomial pathogen Enterococcus faecium and identification of a large transferable pathogenicity island
    Willem van Schaik
    Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    BMC Genomics 11:239. 2010
    The Gram-positive bacterium Enterococcus faecium is an important cause of nosocomial infections in immunocompromized patients.
  4. doi Mobile genetic elements and their contribution to the emergence of antimicrobial resistant Enterococcus faecalis and Enterococcus faecium
    K Hegstad
    Reference Centre for Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway
    Clin Microbiol Infect 16:541-54. 2010
    ..are pivotal in the dissemination and persistence of antimicrobial resistance in Enterococcus faecalis and Enterococcus faecium. Enterococcal MGEs have also been shown to be able to transfer resistance determinants to more pathogenic ..
  5. pmc Distribution of genes encoding MSCRAMMs and Pili in clinical and natural populations of Enterococcus faecium
    Jouko Sillanpaa
    Department of Internal Medicine, Division of Infectious Diseases, University of Texas Medical School at Houston, Houston, TX 77030, USA
    J Clin Microbiol 47:896-901. 2009
    b>Enterococcus faecium has recently emerged as an important cause of nosocomial infections...
  6. doi Transition of Enterococcus faecium from commensal organism to nosocomial pathogen
    Rob Jl Willems
    Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan, CX Utrecht, The Netherlands
    Future Microbiol 4:1125-35. 2009
    The Gram-positive species Enterococcus faecium has long been thought of as a harmless commensal of the mammalian GI tract. In the last two decades, however, E. faecium has become an important cause of nosocomial bacteremias...
  7. doi A genetic element present on megaplasmids allows Enterococcus faecium to use raffinose as carbon source
    Xinglin Zhang
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    Environ Microbiol 13:518-28. 2011
    b>Enterococcus faecium is a commensal of the gastrointestinal tract of humans and animals. Since the 1990s, it has also emerged as a nosocomial pathogen. Little is known about carbon metabolism of E...
  8. pmc Restricted gene flow among hospital subpopulations of Enterococcus faecium
    Rob J L Willems
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    MBio 3:e00151-12. 2012
    b>Enterococcus faecium has recently emerged as an important multiresistant nosocomial pathogen...
  9. pmc Management of multidrug-resistant enterococcal infections
    C A Arias
    Department of Internal Medicine, Division of Infectious Diseases and Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School at Houston, Houston, TX, USA
    Clin Microbiol Infect 16:555-62. 2010
    ..The increased prevalence and dissemination of multidrug-resistant Enterococcus faecium worldwide has resulted in a major decrease in therapeutic options because the majority of E...
  10. pmc Genome-wide identification of ampicillin resistance determinants in Enterococcus faecium
    Xinglin Zhang
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS Genet 8:e1002804. 2012
    b>Enterococcus faecium has become a nosocomial pathogen of major importance, causing infections that are difficult to treat owing to its multi-drug resistance...
  11. ncbi Identification of high-risk enterococcal clonal complexes: global dispersion and antibiotic resistance
    Helen L Leavis
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    Curr Opin Microbiol 9:454-60. 2006
    Vancomycin-resistant Enterococcus faecium spread dramatically in hospital settings in the USA in the 1990s and reached endemicity at the turn of the century...
  12. pmc Analysis of PBP5 of early U.S. isolates of Enterococcus faecium: sequence variation alone does not explain increasing ampicillin resistance over time
    Jessica R Galloway-Peña
    Division of Infectious Disease, Department of Medicine, Center for the Study of Emerging and Re Emerging Pathogens, University of Texas Medical School, Houston, Texas 77030, USA
    Antimicrob Agents Chemother 55:3272-7. 2011
    ..S. Enterococcus faecium isolates...
  13. pmc Contribution of the enterococcal surface protein Esp to pathogenesis of Enterococcus faecium endocarditis
    Esther Heikens
    Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    Microbes Infect 13:1185-90. 2011
    The enterococcal surface protein Esp, specifically linked to nosocomial Enterococcus faecium, is involved in biofilm formation. To assess the role of Esp in endocarditis, a biofilm-associated infection, an Esp-expressing E...
  14. pmc D-Ala-d-Ser VanN-type transferable vancomycin resistance in Enterococcus faecium
    Francois Lebreton
    Interactions Hôte et Microorganismes des Epithéliums, EA2128, CHU Cote de Nacre, 14033 Caen Cedex, France
    Antimicrob Agents Chemother 55:4606-12. 2011
    b>Enterococcus faecium UCN71, isolated from a blood culture, was resistant to low levels of vancomycin (MIC, 16 μg/ml) but susceptible to teicoplanin (MIC, 0.5 μg/ml)...
  15. pmc Analysis of clonality and antibiotic resistance among early clinical isolates of Enterococcus faecium in the United States
    Jessica R Galloway-Peña
    Department of Internal Medicine, Division of Infectious Diseases, University of Texas Medical School at Houston, 6431 Fannin Street, Houston, TX 77030, USA
    J Infect Dis 200:1566-73. 2009
    The Enterococcus faecium genogroup, referred to as clonal complex 17 (CC17), seems to possess multiple determinants that increase its ability to survive and cause disease in nosocomial environments.
  16. doi Genome-based insights into the evolution of enterococci
    W van Schaik
    Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan, Utrecht, The Netherlands
    Clin Microbiol Infect 16:527-32. 2010
    ..In this review, we discuss recent progress on the genomics of Enterococcus faecalis and Enterococcus faecium, which are the two enterococcal species that cause the large majority of enterococcal infections...
  17. doi Pathogenesis and immunity in enterococcal infections
    I G Sava
    Division of Infectious Diseases, Department of Medicine, University Medical Center Freiburg, Germany
    Clin Microbiol Infect 16:533-40. 2010
    Enterococcus faecalis and Enterococcus faecium have emerged as multi-resistant nosocomial pathogens in immunocompromised and critically ill patients...
  18. pmc The recombinase IntA is required for excision of esp-containing ICEEfm1 in Enterococcus faecium
    Janetta Top
    Department of Medical Microbiology, University Medical Center Utrecht, 3584CX Utrecht, Netherlands
    J Bacteriol 193:1003-6. 2011
    Comparative genome analysis of Enterococcus faecium recently revealed that a genomic island containing the esp gene, referred to as the esp-containing pathogenicity island (esp PAI), can be transferred by conjugation and contains a ..
  19. pmc Molecular epidemiology of vancomycin-resistant Enterococcus faecium: a prospective, multicenter study in South American hospitals
    Diana Panesso
    Molecular Genetics and Antimicrobial Resistance Unit, Universidad El Bosque, Bogota, Colombia
    J Clin Microbiol 48:1562-9. 2010
    b>Enterococcus faecium has emerged as an important nosocomial pathogen worldwide, and this trend has been associated with the dissemination of a genetic lineage designated clonal cluster 17 (CC17)...
  20. pmc Importance of two Enterococcus faecium loci encoding Gls-like proteins for in vitro bile salts stress response and virulence
    Tina Choudhury
    Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School at Houston, Texas 77030, USA
    J Infect Dis 203:1147-54. 2011
    ..Here, we identified 2 gene clusters in Enterococcus faecium each encoding a homolog of Gls24 (Gls33 and Gls20; designated on the basis of their predicted sizes) and of ..
  21. pmc Enterococcal surface protein Esp is important for biofilm formation of Enterococcus faecium E1162
    Esther Heikens
    Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    J Bacteriol 189:8233-40. 2007
    ..A single clonal lineage of Enterococcus faecium, complex 17 (CC17), appears to be a successful nosocomial pathogen, and most CC17 isolates harbor the ..
  22. pmc Comparative analysis of the first complete Enterococcus faecium genome
    Margaret M C Lam
    Department of Microbiology and Immunology, University of Melbourne, Victoria, Australia
    J Bacteriol 194:2334-41. 2012
    ..In particular, infections caused by vancomycin-resistant Enterococcus faecium are becoming increasingly common. Comparative and functional genomic studies of E...
  23. pmc IS element IS16 as a molecular screening tool to identify hospital-associated strains of Enterococcus faecium
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    BMC Infect Dis 11:80. 2011
    Hospital strains of Enterococcus faecium could be characterized and typed by various molecular methods (MLST, AFLP, MLVA) and allocated to a distinct clonal complex known as MLST CC17...
  24. pmc Genomic and SNP analyses demonstrate a distant separation of the hospital and community-associated clades of Enterococcus faecium
    Jessica Galloway-Peña
    Division of Infectious Disease, Department of Medicine, University of Texas Medical School, Houston, Texas, United States of America
    PLoS ONE 7:e30187. 2012
    Recent studies have pointed to the existence of two subpopulations of Enterococcus faecium, one containing primarily commensal/community-associated (CA) strains and one that contains most clinical or hospital-associated (HA) strains, ..
  25. pmc Multiple-drug resistant enterococci: the nature of the problem and an agenda for the future
    M M Huycke
    University of Oklahoma Health Sciences Center 73190, USA
    Emerg Infect Dis 4:239-49. 1998
    ..Equally important is renewed vigor in the search for additional drugs, accompanied by the evolution of new therapeutic paradigms less vulnerable to the cycle of drug introduction and drug resistance...
  26. pmc Insertion sequence-driven diversification creates a globally dispersed emerging multiresistant subspecies of E. faecium
    Helen L Leavis
    Eijkman Winkler Institute for Medical Microbiology, Infectious Diseases and Inflammation, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS Pathog 3:e7. 2007
    b>Enterococcus faecium, an ubiquous colonizer of humans and animals, has evolved in the last 15 years from an avirulent commensal to the third most frequently isolated nosocomial pathogen among intensive care unit patients in the United ..
  27. pmc Global spread of the hyl(Efm) colonization-virulence gene in megaplasmids of the Enterococcus faecium CC17 polyclonal subcluster
    Ana R Freitas
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Carretera de Colmenar, Madrid 28034, Spain
    Antimicrob Agents Chemother 54:2660-5. 2010
    b>Enterococcus faecium has increasingly been reported as a nosocomial pathogen since the early 1990s, presumptively associated with the expansion of a human-associated Enterococcus faecium polyclonal subcluster known as clonal complex 17 (..
  28. doi Expression of two distinct types of pili by a hospital-acquired Enterococcus faecium isolate
    Antoni P A Hendrickx
    Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, Rm G04 614, 3584 CX, Utrecht, The Netherlands
    Microbiology 154:3212-23. 2008
    ..distinct pilus-like structures, designated PilA and PilB, on the surface of a hospital-adapted Enterococcus faecium bloodstream isolate. E...
  29. pmc Bacteriophage therapy rescues mice bacteremic from a clinical isolate of vancomycin-resistant Enterococcus faecium
    Biswajit Biswas
    National Institutes of Health, Bethesda, Maryland 20892, USA
    Infect Immun 70:204-10. 2002
    Colonization of the gastrointestinal tract with vancomycin-resistant Enterococcus faecium (VRE) has become endemic in many hospitals and nursing homes in the United States...
  30. ncbi Ecological replacement of Enterococcus faecalis by multiresistant clonal complex 17 Enterococcus faecium
    J Top
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Centre Utrecht, Utrecht, The Netherlands
    Clin Microbiol Infect 13:316-9. 2007
    The proportion of enterococcal infections caused by ampicillin-resistant Enterococcus faecium (AREfm) in a European hospital increased from 2% in 1994 to 32% in 2005, with prevalence rates of AREfm endemicity of up to 35% in at least six ..
  31. pmc Dogs are a reservoir of ampicillin-resistant Enterococcus faecium lineages associated with human infections
    Peter Damborg
    Department of Disease Biology, Faculty of Life Sciences, University of Copenhagen, Stigbøjlen 4, 1870 Frederiksberg C, Denmark
    Appl Environ Microbiol 75:2360-5. 2009
    Ampicillin resistance is a marker for hospital-associated Enterococcus faecium. Feces from 208 dogs were selectively screened for the occurrence of ampicillin-resistant E. faecium (AREF)...
  32. doi Factors affecting the reversal of antimicrobial-drug resistance
    Pål J Johnsen
    Department of Pharmacy, Faculty of Medicine, University of Tromsø, Tromsø, Norway
    Lancet Infect Dis 9:357-64. 2009
    ..Resistance determinants may persist at low, but detectable, levels for many years in the absence of the corresponding drugs...
  33. pmc SgrA, a nidogen-binding LPXTG surface adhesin implicated in biofilm formation, and EcbA, a collagen binding MSCRAMM, are two novel adhesins of hospital-acquired Enterococcus faecium
    Antoni P A Hendrickx
    Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, Rm G04 614, 3584 CX Utrecht, The Netherlands
    Infect Immun 77:5097-106. 2009
    Hospital-acquired Enterococcus faecium isolates responsible for nosocomial outbreaks and invasive infections are enriched in the orf2351 and orf2430 genes, encoding the SgrA and EcbA LPXTG-like cell wall-anchored proteins, respectively...
  34. doi Emergence of CC17 Enterococcus faecium: from commensal to hospital-adapted pathogen
    Janetta Top
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    FEMS Immunol Med Microbiol 52:297-308. 2008
    For many years, Enterococcus faecium was considered to be a commensal of the digestive tract, which only sporadically caused opportunistic infections in severely ill patients. Over the last two decades, vancomycin-resistant E...
  35. pmc Identification and phenotypic characterization of a second collagen adhesin, Scm, and genome-based identification and analysis of 13 other predicted MSCRAMMs, including four distinct pilus loci, in Enterococcus faecium
    Jouko Sillanpaa
    Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, TX, USA
    Microbiology 154:3199-211. 2008
    Attention has recently been drawn to Enterococcus faecium because of an increasing number of nosocomial infections caused by this species and its resistance to multiple antibacterial agents...
  36. ncbi Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark
    F M Aarestrup
    Danish Veterinary Laboratory, 27 Bülowsvej, DK 1790 V, Copenhagen, Denmark
    Diagn Microbiol Infect Dis 37:127-37. 2000
    ..However, similar resistance patterns and resistance genes were detected frequently indicating that transmission of resistant enterococci or resistance genes takes place between humans, broilers, and pigs...
  37. pmc Characterization of the ebp(fm) pilus-encoding operon of Enterococcus faecium and its role in biofilm formation and virulence in a murine model of urinary tract infection
    Jouko Sillanpaa
    Department of Internal Medicine, and Center for the Study of Emerging and Re emerging Pathogens, Division of Infectious Diseases, University of Texas Medical School at Houston, Houston, TX, USA
    Virulence 1:236-46. 2010
    ..identified 15 genes encoding putative surface proteins with features of MSCRAMMs and/or pili in the Enterococcus faecium TX0016 (DO) genome, including four predicted pilus-encoding gene clusters; we also demonstrated that one of ..
  38. doi Enterococcal surface protein transiently aggravates Enterococcus faecium-induced urinary tract infection in mice
    Masja Leendertse
    Center for Infection and Immunity Amsterdam, Academic Medical Center, Amsterdam, The Netherlands
    J Infect Dis 200:1162-5. 2009
    The role that the enterococcal surface protein Esp plays in the capacity of Enterococcus faecium to adhere to uroepithelial cells and the role that it plays in urinary tract infection and peritonitis was investigated in vitro and in vivo, ..
  39. ncbi A potential virulence gene, hylEfm, predominates in Enterococcus faecium of clinical origin
    Louis B Rice
    Research and Medical Services, Louis Stokes Cleveland Veterans Affairs Medical Center and Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
    J Infect Dis 187:508-12. 2003
    ..with homologies to previously described hyaluronidase genes has been identified in nonstool isolates of Enterococcus faecium. E...
  40. doi PCR-based plasmid typing in Enterococcus faecium strains reveals widely distributed pRE25-, pRUM-, pIP501- and pHTbeta-related replicons associated with glycopeptide resistance and stabilizing toxin-antitoxin systems
    Torill C S Rosvoll
    Department of Medical Biology, University of Tromsø, Tromsø, Norway
    FEMS Immunol Med Microbiol 58:254-68. 2010
    ..was applied to identify plasmids in an epidemiologically and geographically diverse strain collection of Enterococcus faecium (n=93)...
  41. doi Enterococcal surface protein contributes to persistence in the host but is not a target of opsonic and protective antibodies in Enterococcus faecium infection
    I G Sava
    Division of Infectious Diseases, Department of Medicine, University Medical Center, Hugstetter Str 55, 79106 Freiburg, Germany
    J Med Microbiol 59:1001-4. 2010
    ..In the past, the majority of infections were caused by Enterococcus faecalis; however, an increase in Enterococcus faecium clinical isolates has been observed in recent years...
  42. pmc AsrR is an oxidative stress sensing regulator modulating Enterococcus faecium opportunistic traits, antimicrobial resistance, and pathogenicity
    Francois Lebreton
    University of Caen Basse Normandie, EA4655 team Antibioresistance, Medical School, Caen, France
    PLoS Pathog 8:e1002834. 2012
    ..an oxidative stress sensor and response regulator in the important multidrug-resistant nosocomial pathogen Enterococcus faecium belonging to the MarR family and called AsrR (antibiotic and stress response regulator)...
  43. pmc RNase 7 contributes to the cutaneous defense against Enterococcus faecium
    Bente Köten
    Department of Dermatology, University Hospital Schleswig Holstein, Campus Kiel, Kiel, Germany
    PLoS ONE 4:e6424. 2009
    ..Because RNase 7 exhibits high activity against Enterococcus faecium the aim of this study was to further explore the role of RNase 7 in the cutaneous innate defense system ..
  44. pmc The hylEfm gene in pHylEfm of Enterococcus faecium is not required in pathogenesis of murine peritonitis
    Diana Panesso
    Department of Internal Medicine, Division of Infectious Diseases, Center for the Study of Emerging and Reemerging Pathogens, Houston, TX, USA
    BMC Microbiol 11:20. 2011
    ..hylEfm (pHylEfm) were previously shown to increase gastrointestinal colonization and lethality of Enterococcus faecium in experimental peritonitis...
  45. doi Optimizing future treatment of enterococcal infections: attacking the biofilm?
    Fernanda L Paganelli
    Department of Medical Microbiology, University Medical Center Utrecht, The Netherlands
    Trends Microbiol 20:40-9. 2012
    Enterococcus faecalis and Enterococcus faecium are among the leading causative agents of nosocomial infections and are infamous for their resistance to many antibiotics...
  46. ncbi Virulence factors of Enterococcus faecalis and Enterococcus faecium blood culture isolates
    H A Elsner
    Abteilung für Transfusionsmedizin, Medizinische Hochschule Hannover, Germany
    Eur J Clin Microbiol Infect Dis 19:39-42. 2000
    ..virulence factors of enterococcal blood culture isolates were studied using 89 Enterococcus faecalis and 24 Enterococcus faecium isolates. The prevalence of the respective factors was (Enterococcus faecalis vs...
  47. pmc vanM, a new glycopeptide resistance gene cluster found in Enterococcus faecium
    Xiaogang Xu
    Institute of Antibiotics, Huashan Hospital, Fudan University, 12 M Wulumuqi Rd, Shanghai, P R China
    Antimicrob Agents Chemother 54:4643-7. 2010
    ..We investigated a clinical strain of Enterococcus faecium Efm-HS0661 that was isolated in 2006 from an inpatient with intra-abdominal infection in Shanghai...
  48. ncbi Clinical isolates of Enterococcus faecium exhibit strain-specific collagen binding mediated by Acm, a new member of the MSCRAMM family
    Sreedhar R Nallapareddy
    Division of Infectious Diseases, Department of Internal Medicine and Center for the Study of Emerging and Re emerging Pathogens, University of Texas Medical School at Houston, 6431 Fannin Street, Houston, TX 77030, USA
    Mol Microbiol 47:1733-47. 2003
    A collagen-binding adhesin of Enterococcus faecium, Acm, was identified...
  49. pmc Impact of specific pbp5 mutations on expression of beta-lactam resistance in Enterococcus faecium
    Louis B Rice
    Medical and Research Services, Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio 44106, USA
    Antimicrob Agents Chemother 48:3028-32. 2004
    We tested the impact of individual PBP 5 mutations on expression of ampicillin resistance in Enterococcus faecium using a shuttle plasmid designed to facilitate expression of cloned pbp5 in ampicillin-susceptible E. faecium D344SRF...
  50. doi Galleria mellonella as a model for studying Enterococcus faecium host persistence
    Francois Lebreton
    EA2128, Service de Microbiologie, Centre Hospitalier Universitaire, Universite de Caen Basse Normandie, Caen, France
    J Mol Microbiol Biotechnol 21:191-6. 2011
    b>Enterococcus faecium is an opportunistic pathogen responsible for numerous outbreaks worldwide. The basis for the colonization capacities, host persistence and environmental stress response of the hospital-adapted clones emerging from E...
  51. pmc High-level ciprofloxacin resistance from point mutations in gyrA and parC confined to global hospital-adapted clonal lineage CC17 of Enterococcus faecium
    Helen L Leavis
    Department of Internal Medicine, Division of Infectious Diseases and Internal Medicine, Eijkman Winkler Institute, University Medical Center Utrecht, Heidelberglaan 100, Rm G04 614, 3584CX Utrecht, The Netherlands
    J Clin Microbiol 44:1059-64. 2006
    To substantiate a common genetic background of ciprofloxacin-resistant Enterococcus faecium, 32 ciprofloxacin-resistant (Cip(r)) and 31 ciprofloxacin-susceptible (Cip(s)) isolates from outbreaks, clinical infections, surveillances, and ..
  52. ncbi Phylogenetic analysis of vancomycin-resistant Enterococcus faecium genotypes associated with outbreaks or sporadic infections in Italy
    Maria Grazia Bonora
    Microbiology Section, Department of Pathology, University of Verona, Verona, Italy
    Microb Drug Resist 13:171-7. 2007
    ..and multilocus sequence typing (MLST) were used to investigate the genetic relatedness of a total of 41 Enterococcus faecium isolates from different backgrounds (hospital outbreaks, n = 9; documented sporadic infections, n = 10; ..
  53. doi High-level ciprofloxacin resistance among hospital-adapted Enterococcus faecium (CC17)
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    Int J Antimicrob Agents 35:119-25. 2010
    Hospital-adapted Enterococcus faecium differ from their colonising variants in humans and animals by additional genomic content...
  54. pmc Human and swine hosts share vancomycin-resistant Enterococcus faecium CC17 and CC5 and Enterococcus faecalis CC2 clonal clusters harboring Tn1546 on indistinguishable plasmids
    Ana R Freitas
    REQUIMTE, Laboratorio de Microbiologia, Faculdade de Farmacia, Universidade do Porto, Porto, Portugal
    J Clin Microbiol 49:925-31. 2011
    ..Thirty clonally related Enterococcus faecium clonal complex 5 (CC5) isolates (17 sequence type 6 [ST6], 6 ST5, 5 ST185, 1 ST147, and 1 ST493) were ..
  55. pmc Inducible expression eliminates the fitness cost of vancomycin resistance in enterococci
    Marie Laure Foucault
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 107:16964-9. 2010
    ..the biological cost of VanB-type resistance due to acquisition of conjugative transposon Tn1549 in Enterococcus faecium and Enterococcus faecalis...
  56. pmc A novel putative enterococcal pathogenicity island linked to the esp virulence gene of Enterococcus faecium and associated with epidemicity
    Helen Leavis
    Diagnostic Laboratory for Infectious Diseases, National Institute for Public Health and the Environment, Bilthoven, The Netherlands
    J Bacteriol 186:672-82. 2004
    ..A gene similar to esp has recently been found in Enterococcus faecium isolates recovered from hospitalized patients...
  57. doi A multiresistance megaplasmid pLG1 bearing a hylEfm genomic island in hospital Enterococcus faecium isolates
    Jenny A Laverde Gomez
    Robert Koch Institute, Infectious Diseases, Wernigerode Branch, Burgstraße 37, D 38855 Wernigerode, Germany
    Int J Med Microbiol 301:165-75. 2011
    b>Enterococcus faecium is considered to be a nosocomial pathogen with increasing medical importance...
  58. pmc Identification of a broadly active phage lytic enzyme with lethal activity against antibiotic-resistant Enterococcus faecalis and Enterococcus faecium
    Pauline Yoong
    Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Ave, New York, NY 10021, USA
    J Bacteriol 186:4808-12. 2004
    Enterococcus faecalis and Enterococcus faecium infections are increasingly difficult to treat due to high levels of resistance to antibiotics. PlyV12, a bacteriophage lytic enzyme, was isolated and shown to effectively kill both E...
  59. pmc Five genes encoding surface-exposed LPXTG proteins are enriched in hospital-adapted Enterococcus faecium clonal complex 17 isolates
    Antoni P A Hendrickx
    Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, Rm G04 614, 3584 CX Utrecht, The Netherlands
    J Bacteriol 189:8321-32. 2007
    Most Enterococcus faecium isolates associated with hospital outbreaks and invasive infections belong to a distinct genetic subpopulation called clonal complex 17 (CC17)...
  60. pmc VanA-type enterococci from humans, animals, and food: species distribution, population structure, Tn1546 typing and location, and virulence determinants
    F Biavasco
    Institute of Microbiology and Biomedical Sciences, Polytechnic University of Marche, Via Ranieri, Monte D Ago, 60131 Ancona, Italy
    Appl Environ Microbiol 73:3307-19. 2007
    ..PFGE) revealed two small genetically related clusters, M39 (n=4) and M49 (n=13), representing Enterococcus faecium isolates from animal and human feces and from clinical and fecal human samples...
  61. pmc Dispersion of multidrug-resistant Enterococcus faecium isolates belonging to major clonal complexes in different Portuguese settings
    Ana R Freitas
    REQUIMTE, Laboratorio de Microbiologia, Faculdade de Farmacia, Universidade do Porto, Porto, Portugal
    Appl Environ Microbiol 75:4904-8. 2009
    The population structure of 56 Enterococcus faecium isolates selected from a collection of enterococci from humans, animals, and the environment in Portugal (1997 to 2007) was analyzed by multilocus sequence typing...
  62. pmc Identification of a novel genomic island specific to hospital-acquired clonal complex 17 Enterococcus faecium isolates
    Esther Heikens
    Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    Appl Environ Microbiol 74:7094-7. 2008
    Hospital-acquired clonal complex 17 (CC17) Enterococcus faecium strains are genetically distinct from indigenous strains and are enriched with resistance genes and virulence genes. We identified a genomic island in CC17 E...
  63. ncbi Vancomycin-resistant enterococci: why are they here, and where do they come from?
    M J Bonten
    Department of Internal Medicine and the Eijkman Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, University Medical Center Utrecht, The Netherlands
    Lancet Infect Dis 1:314-25. 2001
    ....
  64. pmc Development of a multiplex PCR for the detection of asa1, gelE, cylA, esp, and hyl genes in enterococci and survey for virulence determinants among European hospital isolates of Enterococcus faecium
    Vanessa Vankerckhoven
    Laboratory of Medical Microbiology, University of Antwerp, Antwerp, Belgium
    J Clin Microbiol 42:4473-9. 2004
    ..The presence of these genes was investigated in 153 clinical and 118 fecal Enterococcus faecium isolates from inpatients at an increased risk of developing infections (such as patients in intensive care ..
  65. doi Clonal spread of CC17 vancomycin-resistant Enterococcus faecium with multilocus sequence type 78 (ST78) and a novel ST444 in Taiwan
    Y C Hsieh
    Division of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Children s Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan
    Eur J Clin Microbiol Infect Dis 29:25-30. 2010
    From May 2007 to January 2008, 30 isolates of vancomycin-resistant enterococci (VRE), including 29 Enterococcus faecium (96.7%) and 1 E. faecalis (3...
  66. pmc Influence of a probiotic strain of Enterococcus faecium on Salmonella enterica serovar Typhimurium DT104 infection in a porcine animal infection model
    Istvan Szabo
    Federal Institute for Risk Assessment, Division of Biological Safety, Diedersdorfer Weg 1, 12277 Berlin, Germany
    Appl Environ Microbiol 75:2621-8. 2009
    ..Here, we investigated the influence of oral treatment of pigs with the probiotic bacterium Enterococcus faecium NCIMB 10415 on Salmonella enterica serovar Typhimurium DT104 infections in weaning piglets...
  67. doi Epidemiological link between wastewater and human vancomycin-resistant Enterococcus faecium isolates
    Malihe Talebi
    Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
    Curr Microbiol 56:468-73. 2008
    ..Out of the total samples, 38 Enterococcus faecium (3.6%) from sewage (n = 19) and clinical (n = 19) isolates were found to be highly resistant to vancomycin...
  68. doi Host range of enterococcal vanA plasmids among Gram-positive intestinal bacteria
    Guido Werner
    Robert Koch Institute, Wernigerode, Germany
    J Antimicrob Chemother 66:273-82. 2011
    ..glycopeptide resistance is encoded by the vanA transposon Tn1546 located mainly on transferable plasmids in Enterococcus faecium. The limited occurrence in other species could be due to the lack of inter-species transferability and/or ..
  69. ncbi Linkage of erm(B) and aadE-sat4-aphA-3 in multiple-resistant Enterococcus faecium isolates of different ecological origins
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, D 38855 Wernigerode, Germany
    Microb Drug Resist 9:S9-16. 2003
    b>Enterococcus faecium shares its antibiotic resistance gene pool with various Gram-positive bacteria. A gene cluster aadE-sat4-aphA-3, which was first described in staphylococci, has been recently identified also in E. faecium...
  70. pmc Hospital and community ampicillin-resistant Enterococcus faecium are evolutionarily closely linked but have diversified through niche adaptation
    Marieke J A de Regt
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 7:e30319. 2012
    Ampicillin-resistant Enterococcus faecium (ARE) has emerged as a nosocomial pathogen. Here, we quantified ARE carriage in different community sources and determined genetic relatedness with hospital ARE.
  71. ncbi In vitro activity of phenothiazine derivatives in Enterococcus faecalis and Enterococcus faecium
    Oliver Hendricks
    Department of Research and Department of Microbiology, University of Southern Denmark, Sønderborg, Denmark
    Basic Clin Pharmacol Toxicol 96:33-6. 2005
    ..thioridazine and prochlorperazine have been evaluated with 11 Enterococcus faecalis strains and 9 Enterococcus faecium strains, originating from human infections and animal faecal flora. We found that all E. faecalis and E...
  72. pmc Prevalence, persistence, and molecular characterization of glycopeptide-resistant enterococci in Norwegian poultry and poultry farmers 3 to 8 years after the ban on avoparcin
    M Sørum
    Department of Microbiology and Virology, Faculty of Medicine, University and University Hospital of North Norway, Tromsø, Norway
    Appl Environ Microbiol 72:516-21. 2006
    ..Throughout the study period, 78.5% of the poultry samples were GRE positive. Glycopeptide-resistant Enterococcus faecium (GREF) was isolated from 27.6% of the farmer samples in 1998 and from 27...
  73. ncbi Effect of banning vancomycin analogue avoparcin on vancomycin-resistant enterococci in chicken farms in Taiwan
    Tsai Ling Lauderdale
    Division of Clinical Research, National Health Research Institutes, Zhunan, Taiwan
    Environ Microbiol 9:819-23. 2007
    ....
  74. pmc Construction of improved temperature-sensitive and mobilizable vectors and their use for constructing mutations in the adhesin-encoding acm gene of poorly transformable clinical Enterococcus faecium strains
    Sreedhar R Nallapareddy
    Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School at Houston, 6431 Fannin St, MSB 2 112, Houston, TX 77030, USA
    Appl Environ Microbiol 72:334-45. 2006
    Inactivation by allelic exchange in clinical isolates of the emerging nosocomial pathogen Enterococcus faecium has been hindered by lack of efficient tools, and, in this study, transformation of clinical isolates was found to be ..
  75. ncbi Molecular analysis of the 21-kb bacteriocin-encoding plasmid pEF1 from Enterococcus faecium 6T1a
    José Luis Ruiz-Barba
    Departamento de Biotecnologia de Alimentos, Instituto de la Grasa, Consejo Superior de Investigaciones Cientificas, Avda Padre Garcia Tejero, 4 Aptdo 1078, 41012 Sevilla, Spain
    Plasmid 57:175-81. 2007
    The complete 21,344-bp DNA sequence of the bacteriocin-encoding plasmid pEF1 from Enterococcus faecium 6T1a was determined. Thirty-four putative open reading frames which could code for proteins longer than 42 amino acids were found...
  76. pmc Molecular structure and transferability of Tn1546-like elements in Enterococcus faecium isolates from clinical, sewage, and surface water samples in Iran
    M Talebi
    Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
    Appl Environ Microbiol 74:1350-6. 2008
    The molecular structure and transferability of Tn1546 in 143 vancomycin-resistant Enterococcus faecium (VREF) isolates obtained from patients (n = 49), surface water (n = 28), and urban and hospital sewage (n = 66) in Tehran, Iran, were ..
  77. pmc Emergence of clonal complex 17 Enterococcus faecium in The Netherlands
    Janetta Top
    Department of Medical Microbiology, University Medical Center Utrecht, G04 614, P O Box 85500, 3508 GA Utrecht, The Netherlands
    J Clin Microbiol 46:214-9. 2008
    The global emergence of vancomycin-resistant Enterococcus faecium has been characterized as the clonal spread of clonal complex 17 (CC17) E. faecium...
  78. pmc Differences in antibiotic resistance patterns of Enterococcus faecalis and Enterococcus faecium strains isolated from farm and pet animals
    P Butaye
    Laboratory of Veterinary Bacteriology and Mycology, Department of Pathology, Bacteriology, and Poultry Diseases, Faculty of Veterinary Medicine, University of Ghent, B 9820 Merelbeke, Belgium
    Antimicrob Agents Chemother 45:1374-8. 2001
    The prevalence of acquired resistance in 146 Enterococcus faecium and 166 Enterococcus faecalis strains from farm and pet animals, isolated in 1998 and 1999 in Belgium, against antibiotics used for growth promotion and for therapy was ..
  79. pmc Efficient transfer of the pheromone-independent Enterococcus faecium plasmid pMG1 (Gmr) (65.1 kilobases) to Enterococcus strains during broth mating
    Y Ike
    Department of Microbiology, Gunma University School of Medicine, Maebashi, Gunma, Japan
    J Bacteriol 180:4886-92. 1998
    Plasmid pMG1 (65.1 kb) was isolated from a gentamicin-resistant Enterococcus faecium clinical isolate and was found to encode gentamicin resistance...
  80. pmc Characterization of Tn1546 in vancomycin-resistant Enterococcus faecium isolated from canine urinary tract infections: evidence of gene exchange between human and animal enterococci
    S Simjee
    Center for Veterinary Medicine, U S Food and Drug Administration, Laurel, Maryland 20708, USA
    J Clin Microbiol 40:4659-65. 2002
    ..Isolated species included Enterococcus faecium (n = 13), Enterococcus faecalis (n = 7), Enterococcus gallinarum (n = 11), and Enterococcus casseliflavus (..
  81. doi Suitability of silica hydride stationary phase, aqueous normal phase chromatography for untargeted metabolomic profiling of Enterococcus faecium and Staphylococcus aureus
    Scott A Weisenberg
    Department of Medicine, Division of Infectious Diseases, Weill Cornell Medical College, NY 10065, USA
    J Sep Sci 32:2262-5. 2009
    ..chromatography to the chemical complexity of the intracellular metabolomes of Staphylococcus aureus and Enterococcus faecium. We specifically demonstrate that the chromatographic behavior of known metabolites is unaffected by the ..
  82. ncbi Association between the use of avilamycin for growth promotion and the occurrence of resistance among Enterococcus faecium from broilers: epidemiological study and changes over time
    F M Aarestrup
    Danish Veterinary Laboratory, Copenhagen
    Microb Drug Resist 6:71-5. 2000
    This study describes the changes in the occurrence of resistance to avilamycin among Enterococcus faecium from broilers in Denmark and the epidemiological association between usage of avilamycin for growth promotion and the occurrence of ..
  83. ncbi Transmission of opportunistic pathogens in a veterinary teaching hospital
    P Boerlin
    Institute for Veterinary Bacteriology, University of Bern, Langgass strasse 122, CH 3012, Bern, Switzerland
    Vet Microbiol 82:347-59. 2001
    Acinetobacter baumannii, Enterococcus faecalis, Enterococcus faecium, and Staphylococcus intermedius isolates from infected surgical wounds and other types of infections in a veterinary teaching hospital were typed by pulsed field gel ..
  84. doi A sustained hospital outbreak of vancomycin-resistant Enterococcus faecium bacteremia due to emergence of vanB E. faecium sequence type 203
    Paul D R Johnson
    Department of Infectious Diseases and Microbiology, Austin Health, Monash University, Melbourne, Victoria, Australia
    J Infect Dis 202:1278-86. 2010
    A significant increase in the rate of vancomycin-resistant Enterococcus faecium (VREfm) bacteremia at our health service, despite improved infection control, prompted us to investigate the cause.
  85. pmc Genotyping of Enterococcus faecalis and Enterococcus faecium isolates by use of a set of eight single nucleotide polymorphisms
    I U Rathnayake
    Cell and Molecular Biosciences, Faculty of Science and Technology, Queensland University of Technology, 2 George St, Brisbane, Queensland 4001, Australia
    J Clin Microbiol 49:367-72. 2011
    A single nucleotide polymorphism (SNP) genotyping method for Enterococcus faecalis and Enterococcus faecium was developed using the "Minimum SNPs" program. SNP sets were interrogated using allele-specific real-time PCR...
  86. doi Daptomycin for the treatment of enterococcal bacteraemia: results from the Cubicin Outcomes Registry and Experience (CORE)
    John F Mohr
    Cubist Pharmaceuticals, Inc, 65 Hayden Avenue, Lexington, MA 02421, USA
    Int J Antimicrob Agents 33:543-8. 2009
    ..Outcome was assessed using protocol-defined criteria. Of 159 patients included in the efficacy population, Enterococcus faecium and Enterococcus faecalis were isolated in 120 (75.5%) and 39 (24.5%) patients, respectively...
  87. pmc In vivo transfer of the vanA resistance gene from an Enterococcus faecium isolate of animal origin to an E. faecium isolate of human origin in the intestines of human volunteers
    Camilla H Lester
    National Center for Antimicrobials and Infection Control, Statens Serum Institut, 5 Artillerivej, DK 2300 Copenhagen S, Denmark
    Antimicrob Agents Chemother 50:596-9. 2006
    ..Six volunteers ingested a vancomycin-resistant Enterococcus faecium isolate of chicken origin, together with a vancomycin-susceptible E. faecium recipient of human origin...
  88. pmc Comparison of an automated repetitive-sequence-based PCR microbial typing system with pulsed-field gel electrophoresis for molecular typing of vancomycin-resistant Enterococcus faecium
    Yu Chung Chuang
    Department of Internal Medicine, National Taiwan University Hospital, No 7 Chung Shan South Road, Taipei, Taiwan
    J Clin Microbiol 48:2897-901. 2010
    Vancomycin-resistant Enterococcus faecium (VRE) has become an important health care-associated pathogen because of its rapid spread, limited therapeutic options, and possible transfer of vancomycin resistance to more-virulent pathogens...
  89. pmc Cotransfer of antibiotic resistance genes and a hylEfm-containing virulence plasmid in Enterococcus faecium
    Cesar A Arias
    Department of Internal Medicine, Division of Infectious Diseases, University of Texas Medical School at Houston, Houston, TX 77030, USA
    Antimicrob Agents Chemother 53:4240-6. 2009
    The hyl(Efm) gene (encoding a putative hyaluronidase) has been found almost exclusively in Enterococcus faecium clinical isolates, and recently, it was shown to be on a plasmid which increased the ability of E...
  90. ncbi Correlation between enterococcal biofilm formation in vitro and medical-device-related infection potential in vivo
    Jonathan A T Sandoe
    Department of Microbiology, The General Infirmary at Leeds and University of Leeds, Leeds LS1 3EX, UK
    J Med Microbiol 52:547-50. 2003
    ..Biofilm formation on microplates was demonstrated by all Enterococcus faecalis isolates and 16/38 (42 %) Enterococcus faecium isolates. E. faecalis isolates produced significantly more biofilm than E...
  91. ncbi Antimicrobial resistance and molecular epidemiology of vancomycin-resistant enterococci from North America and Europe: a report from the SENTRY antimicrobial surveillance program
    Lalitagauri M Deshpande
    JMI Laboratories, North Liberty, IA 52317, USA
    Diagn Microbiol Infect Dis 58:163-70. 2007
    ..Most of the submitted isolates were Enterococcus faecium (91.0%) and Enterococcus faecalis (7.8%)...
  92. pmc High-level vancomycin-resistant Staphylococcus aureus isolates associated with a polymicrobial biofilm
    Linda M Weigel
    NCID DHQP ELB MS G 08, Centers for Disease Control and Prevention, 1600 Clifton Road, N E, Atlanta, GA 30333, USA
    Antimicrob Agents Chemother 51:231-8. 2007
    ..S. aureus, Enterococcus faecalis, Enterococcus faecium, Micrococcus species, Morganella morganii, and Pseudomonas aeruginosa were isolated from the biofilm...
  93. ncbi Glycopeptide-resistant enterococci: deciphering virulence, resistance and epidemicity
    Rob J L Willems
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    Curr Opin Infect Dis 20:384-90. 2007
    ..In this review the most recent findings that relate to enterococcal epidemiology, virulence and glycopeptide-resistance maintenance will be discussed...
  94. pmc The current MLVA typing scheme for Enterococcus faecium is less discriminatory than MLST and PFGE for epidemic-virulent, hospital-adapted clonal types
    Guido Werner
    Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    BMC Microbiol 7:28. 2007
    ..repeat analysis) is a reliable typing technique introduced recently to differentiate also isolates of Enterococcus faecium. We used the established VNTR (variable number of tandem repeats) scheme to test its suitability to ..
  95. ncbi Enterococcus faecium SF68 enhances the immune response to Giardia intestinalis in mice
    J Benyacoub
    Nestle Research Center, CH 1000, Lausanne 26, Switzerland
    J Nutr 135:1171-6. 2005
    We studied the ability of the probiotic organism Enterococcus faecium SF68 to antagonize Giardia intestinalis infection in mice. Oral feeding of E...
  96. pmc Emergence of daptomycin resistance in Enterococcus faecium during daptomycin therapy
    James S Lewis
    Antimicrob Agents Chemother 49:1664-5. 2005
  97. ncbi Effect of subtherapeutic antimicrobials on genetic diversity of Enterococcus faecium from chickens
    Charlene R Jackson
    Bacterial Epidemiology and Antimicrobial Resistance Research Unit, USDA ARS, Russell Research Center, Athens, GA 30605, USA
    Avian Dis 50:115-9. 2006
    ..These results suggest that E. faecium from chicken are genetically diverse and that growth-promoting antimicrobials do not affect the genetic population of E. faecium...
  98. pmc Inhibition of Enterococcus faecium adherence to collagen by antibodies against high-affinity binding subdomains of Acm
    Sreedhar R Nallapareddy
    Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School at Houston, 6431 Fannin Street, MSB 2 112, Houston, TX 77030, USA
    Infect Immun 75:3192-6. 2007
    Strains of Enterococcus faecium express a cell wall-anchored protein, Acm, which mediates adherence to collagen...
  99. pmc Effects of a probiotic strain of Enterococcus faecium on the rate of natural chlamydia infection in swine
    M Pollmann
    Institut für Mikrobiologie und Tierseuchen, Freie Universitat Berlin, Philippstrasse 13, 10115 Berlin, Germany
    Infect Immun 73:4346-53. 2005
    ..Here we performed a study comparing the efficacy of a probiotic strain of Enterococcus faecium on the reduction of both the rate of natural infection and the shedding of chlamydiae in swine...
  100. pmc Possible connection between a widely disseminated conjugative gentamicin resistance (pMG1-like) plasmid and the emergence of vancomycin resistance in Enterococcus faecium
    Haruyoshi Tomita
    Department of Microbiology, Gunma University School of Medicine, Maebashi, Gunma 371 8511, Japan
    J Clin Microbiol 40:3326-33. 2002
    A total of 640 vancomycin-resistant Enterococcus faecium (VRE) isolates, which were obtained between 1994 and 1999 from the Medical School Hospital of the University of Michigan, Ann Arbor, were used in this study...
  101. ncbi Structure of an antigenic teichoic acid shared by clinical isolates of Enterococcus faecalis and vancomycin-resistant Enterococcus faecium
    Y Wang
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115 5804, USA
    Carbohydr Res 316:155-60. 1999
    ..It was composed of glucose, glycerol, and phosphate as determined by chemical and GC-MS analysis. The repeating-unit structure was elucidated by a series of 1H, 13C, and 31P NMR spectroscopy to be the following: [formula: see text]..

Research Grants75

  1. Synbiotics as Adjuvant to Influenza Vaccine in Elderly
    Abinash Virk; Fiscal Year: 2006
    ..with Agri-King Corporation has developed a novel synbiotic called AKSB that contains a probiotic bacterium (Enterococcus faecium), a probiotic yeast (Saccharomyces cerevisiae), and a prebiotic (fructo-oligosaccharide [FOS])...
  2. Ampicillin resistance mechanisms in E. faecium
    Louis Rice; Fiscal Year: 2007
    Multi-resistant Enterococcus faecium cause significant problems for both nosocomial infection treatment and control. Ampicillin is the therapy of choice for enterococcal strains that are susceptible to this agent. E...
  3. Glycopeptide Complexes with Bacterial Cell Walls by REDOR NMR
    Jacob Schaefer; Fiscal Year: 2010
    ..detection of drug-complex formation and biosynthesis in whole cells of wild-type Staphylococcus aureus and Enterococcus faecium. In the next grant period, we plan to characterize the full 3D architecture of the peptidoglycan lattice of ..
  4. Novel antibacterials targeting the 50S ribosomal subunit
    Joyce Sutcliffe; Fiscal Year: 2005
    ..pathogens, a drug must cover Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, and Enterococcus faecium. Coverage of Streptococcus pneumonias (for treatment of patients who are either hospitalized due to ..
  5. HYBRID MOLECULES DESIGNED TO ENHANCE ANTIBIOTIC ACTIVITY
    George Wright; Fiscal Year: 2007
    ..of antibiotic resistant Gram-positive bacteria, especially Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium and Streptococcus pneumoniae, has stimulated discovery of novel agents that can selectively attack new ..
  6. Role of Enterococcal Polysaccharides in Virulence
    Johannes Huebner; Fiscal Year: 2006
    ..biochemical, and immunologic factors that contribute to the pathogenesis of Enterococcus faecalis and Enterococcus faecium infections...
  7. VIRULENCE OF VANCOMYCIN RESISTANT AND OTHER E FAECIUM
    Barbara Murray; Fiscal Year: 2003
    DESCRIPTION (Adapted from investigator's abstract): For serious infections due to vancomycin resistant Enterococcus faecium that are also highly ampicillin resistant, there is no known, effective therapy...
  8. BIO-ORGANIC MECHANISMS OF PEPTIDE ANTIBIOTICS
    Dewey McCafferty; Fiscal Year: 2004
    ..antibiotic with proven activity against methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus faecium, and cephalosporin-resistant Streptococcus pneumonia, three important Gram positive opportunistic human ..
  9. Bioorganic mechanism of peptide antibiotics
    Dewey McCafferty; Fiscal Year: 2007
    ..against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus faecium (VRE), and cephalosporin-resistant Streptococcus pneumonia, three important opportunistic human pathogens...
  10. Bioorganic mechanism of peptide antibiotics
    Dewey McCafferty; Fiscal Year: 2009
    ..against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus faecium (VRE), and cephalosporin-resistant Streptococcus pneumonia, three important opportunistic human pathogens...
  11. Importance of adhesin-like proteins of Enteroccocus faecium
    Barbara Murray; Fiscal Year: 2009
    ..While, in the past, infections due to Enterococcus faecium were infrequent, this organism has increased markedly in frequency and in importance in the hospital ..
  12. Importance of adhesin-like proteins of Enteroccocus faecium
    Barbara E Murray; Fiscal Year: 2010
    ..While, in the past, infections due to Enterococcus faecium were infrequent, this organism has increased markedly in frequency and in importance in the hospital ..
  13. Importance of adhesin-like proteins of Enteroccocus faecium
    Barbara Murray; Fiscal Year: 2007
    ..While, in the past, infections due to Enterococcus faecium were infrequent, this organism has increased markedly in frequency and in importance in the hospital ..
  14. Cell wall synthesis enzymes and beta-lactam resistance in Enterococcus faecium
    Louis B Rice; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: Enterococcus faecium is a common and increasingly important cause of nosocomial infections...
  15. REGULATION OF AMPICILLIN RESISTANCE IN E FACEIUM
    Louis Rice; Fiscal Year: 2004
    ..However, it is equally problematic that virtually all vancomycin-resistant enterococci (VRE) are Enterococcus faecium that express resistance to high levels of ampicillin. While it is clear that ampicillin resistance in E...
  16. Strategies and Tactics for Natural Products Synthesis
    GARY SULIKOWSKI; Fiscal Year: 2007
    ..Lomaiviticins A and B are potent antibiotics against Gram-positive bacteria Staphylococcus aureus and Enterococcus faecium (MIC 6-25 ng/spot)...
  17. Strategies and Tactics for Natural Products Synthesis
    GARY SULIKOWSKI; Fiscal Year: 2007
    ..Lomaiviticins A and B are potent antibiotics against Gram-positive bacteria Staphylococcus aureus and Enterococcus faecium (MIC 6-25 ng/spot)...
  18. TOTAL SYNTHESIS OF KINAMYCINS AND LOMAIVITICINS
    K Nicolaou; Fiscal Year: 2007
    ..The significance of the proposed work will lie specifically in the area of cancer chemotherapy research, and in the development of new synthetic strategies and technologies for general use in the drug discovery and development process. ..
  19. TOTAL SYNTHESIS OF AZASPIRACIDS
    K Nicolaou; Fiscal Year: 2006
    ..g. lung, liver, spleen and lymphocyte damage as well as cancer) health hazards. The project is also expected to advance our knowledge in chemical synthesis and impact favorably the drug discovery and development process. ..
  20. Novel Therapies for BioFilm-Related Infections
    Timothy Opperman; Fiscal Year: 2005
    ..epidermidis. The work in Phase I will provide all the molecular genetic tools and screening methods needed in Phase II for the discovery of potent small molecule inhibitors of staphylococcal biofilm formation. ..
  21. Helicobacter pylori: Pathogenesis and Virulence
    Kenneth Simpson; Fiscal Year: 2005
    ....
  22. THERAPEUTIC APPROACHES TO BIOFILM ANTIBIOTIC RESISTANCE
    Timothy Opperman; Fiscal Year: 2005
    ..We will develop these compounds into innovative drugs that will be used in combination with clinically relevant antibiotics to eradicate biofilm infections. ..
  23. Mentored Patient-Oriented Research Careeer Development Award
    Debra Poutsiaka; Fiscal Year: 2007
    ..abstract_text> ..
  24. c-diGMP Signal Transduction Proteins are Biofilm Drug Targets
    Timothy Opperman; Fiscal Year: 2006
    ..This development will represent a major step forward in the treatment of CF lung disease, and will have a profound economic and social impact; enabling clinicians to better control the biofilm-related infections that plague CF patients. ..