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Genomes and Genes | bradyrhizobiumSummarySummary: A genus of gram-negative, aerobic, rod-shaped bacteria usually containing granules of poly-beta-hydroxybutyrate. They characteristically invade the root hairs of leguminous plants and act as intracellular symbionts. Top Publications
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Publications
Thiosulfate-dependent chemolithoautotrophic growth of Bradyrhizobium japonicumSachiko Masuda
Graduate School of Life Sciences, Tohoku University, 2 1 1 Katahira, Aoba ku, Sendai 980 8577, Japan
Appl Environ Microbiol 76:2402-9. 2010Thiosulfate-oxidizing sox gene homologues were found at four loci (I, II, III, and IV) on the genome of Bradyrhizobium japonicum USDA110, a symbiotic nitrogen-fixing bacterium in soil. In fact, B...
Complete transcriptome of the soybean root hair cell, a single-cell model, and its alteration in response to Bradyrhizobium japonicum infectionMarc Libault
Division of Plant Sciences, National Center for Soybean Biotechnology, CS Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211, USA
Plant Physiol 152:541-52. 2010..g. soybean [Glycine max] and Bradyrhizobium japonicum) initiated by the infection of plant root hair cells by the symbiont...
Isovaleryl-homoserine lactone, an unusual branched-chain quorum-sensing signal from the soybean symbiont Bradyrhizobium japonicumAndrea Lindemann
Department of Microbiology, University of Washington, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 108:16765-70. 2011..Here we report that BjaI from the soybean symbiont Bradyrhizobium japonicum USDA110 is closely related to RpaI and BtaI and catalyzes the synthesis of isovaleryl-HSL (IV-HSL), ..
The PhyR-sigma(EcfG) signalling cascade is involved in stress response and symbiotic efficiency in Bradyrhizobium japonicumBenjamin Gourion
ETH, Institute of Microbiology, Zurich, Switzerland
Mol Microbiol 73:291-305. 2009..Here, we investigate the role of these two regulators in the soybean symbiont Bradyrhizobium japonicum USDA110...
An integrated proteomics and transcriptomics reference data set provides new insights into the Bradyrhizobium japonicum bacteroid metabolism in soybean root nodulesNathanael Delmotte
ETH, Institute of Microbiology, Zurich, Switzerland
Proteomics 10:1391-400. 2010b>Bradyrhizobium japonicum, a gram-negative soil bacterium that establishes an N(2)-fixing symbiosis with its legume host soybean (Glycine max), has been used as a symbiosis model system...
Novel and nodulation-regulated microRNAs in soybean rootsSenthil Subramanian
Donald Danforth Plant Science Center, 975 N Warson Road, St Louis, MO 63132, USA
BMC Genomics 9:160. 2008..Symbiosis between legumes (e.g. soybean) and rhizobia bacteria (e.g. Bradyrhizobium japonicum) results in root nodules where the majority of biological nitrogen fixation occurs...
Bradavidin II from Bradyrhizobium japonicum: a new avidin-like biotin-binding proteinSatu H Helppolainen
Institute of Medical Technology, Biokatu 6, FI 33014 University of Tampere and Tampere University Hospital, Finland
Biochim Biophys Acta 1784:1002-10. 2008..Structural elements of bradavidin II such as an interface residue pattern or biotin contact residues could be used as such or transferred to engineered avidin forms to improve or create new tools for biotechnological applications...
Simultaneous interaction of Arabidopsis thaliana with Bradyrhizobium Sp. strain ORS278 and Pseudomonas syringae pv. tomato DC3000 leads to complex transcriptome changesFabienne Cartieaux
UMR113, Universite Montpellier 2, Institut de Recherche pour le Developpement, CIRAD, Ecole Nationale Supérieure d Agronomie de Montpellier, Institut National de la Recherche Agronomique, CC 002, Place Eugene Bataillon, 34095 Montpellier Cedex 5, France
Mol Plant Microbe Interact 21:244-59. 2008..Here, we show for the first time that a photosynthetic Bradyrhizobium sp...
Enhanced attachment of Bradyrhizobium japonicum to soybean through reduced root colonization of internally seedborne microorganismsN W Oehrle
University of Missouri Columbia, Department of Biochemistry 65211, USA
Can J Microbiol 46:600-6. 2000..soybean (Glycine max) seed, introducing an undefined parameter into studies on attachment and infection by Bradyrhizobium japonicum. Bacterial isolates from surface-sterilized soybean seed, cv. Williams 82 and cv...
Phosphatidylcholine levels in Bradyrhizobium japonicum membranes are critical for an efficient symbiosis with the soybean host plantA C Minder
Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
Mol Microbiol 39:1186-98. 2001..Here, we report on the isolation of a Bradyrhizobium japonicum gene (pmtA) encoding the phospholipid N-methyltransferase PmtA...
Detection of homoserine lactone-like quorum sensing molecules in bradyrhizobium strainsNeelawan Pongsilp
Department of Agronomy, University of Wisconsin-Madison, 53706, USA
Curr Microbiol 51:250-4. 2005One hundred and forty-two Bradyrhizobium strains were screened for their ability to produce N-acyl homoserine lactone-like molecules (AHLs) by using an Agrobacterium tumefaciens biosensor strain containing a traI-lacZ fusion...
Functional characterization of the Bradyrhizobium japonicum modA and modB genes involved in molybdenum transportMaria J Delgado
Departamento de Microbiologia del Suelo y Sistemas Simbioticos, Estacion Experimental del Zaidin, CSIC, PO Box 419, 18080 Granada, Spain
Microbiology 152:199-207. 2006A modABC gene cluster that encodes an ABC-type, high-affinity molybdate transporter from Bradyrhizobium japonicum has been isolated and characterized. B...
A proximal arginine R206 participates in switching of the Bradyrhizobium japonicum FixL oxygen sensorMarie Alda Gilles-Gonzalez
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9038, USA
J Mol Biol 360:80-9. 2006..We assessed the contributions of this residue in Bradyrhizobium japonicum FixL by determining the effects of an R206A substitution on the heme-PAS structure, ligand affinity, ..
Population density-dependent regulation of the Bradyrhizobium japonicum nodulation genesJ T Loh
Center for Legume Research, Department of Microbiology, The University of Tennessee, Knoxville, TN 37996, USA
Mol Microbiol 42:37-46. 2001The nodulation genes of Bradyrhizobium japonicum are essential for infection and establishment of a nitrogen-fixing symbiosis...
Generation of Bradyrhizobium japonicum mutants with increased N2O reductase activity by selection after introduction of a mutated dnaQ geneManabu Itakura
Graduate School of Life Sciences, Tohoku University, Aoba ku, Sendai, Miyagi 980 8577, Japan
Appl Environ Microbiol 74:7258-64. 2008We obtained two beneficial mutants of Bradyrhizobium japonicum USDA110 with increased nitrous oxide (N(2)O) reductase (N(2)OR) activity by introducing a plasmid containing a mutated B...
Identification of active-site residues in Bradyrhizobium japonicum malonyl-coenzyme A synthetaseH M Koo
Department of Biochemistry, College of Science, Bioproducts Research Center, Yonsei University, Seoul, Korea
Arch Biochem Biophys 378:167-74. 2000Malonyl-CoA synthetase (MCS) has been previously purified and characterized from Bradyrhizobium japonicum USDA 110. The gene encoding this enzyme is now cloned, sequenced, and expressed in Escherichia coli...
Analysis of the secretome of the soybean symbiont Bradyrhizobium japonicumJana Hempel
Institut fur Genetik, Technische Universitat Dresden, Dresden, Germany
J Biotechnol 140:51-8. 2009Proteins from the supernatant of Bradyrhizobium japonicum were separated by two-dimensional gel electrophoresis and stained with Coomassie. This revealed more than 100 protein spots...
The major Nod factor of Bradyrhizobium japonicum promotes early growth of soybean and cornA Souleimanov
Department of Plant Science, Macdonald Campus, McGill University, 21, 111 Lakeshore Road, Ste Anne de Bellevue, QC, Canada H9X 3V9
J Exp Bot 53:1929-34. 2002..These results suggest that Nod factor, besides mediating early stages of nodulation, has more general plant growth-promoting effects...
Unexpectedly diverse Mesorhizobium strains and Rhizobium leguminosarum nodulate native legume genera of New Zealand, while introduced legume weeds are nodulated by Bradyrhizobium speciesBevan S Weir
Landcare Research, Private Bag 92170, Auckland, New Zealand
Appl Environ Microbiol 70:5980-7. 2004..In contrast, all sequences from isolates from introduced weeds aligned with Bradyrhizobium species but formed clusters distinct from existing named species...
Effect of Bradyrhizobium photosynthesis on stem nodulation of Aeschynomene sensitivaE Giraud
Laboratoire des Symbioses Tropicales et Méditerranéennes, Institut de Recherche pour le Developpement, Centre de Cooperation Internationale en Recherche Agronomique pour le Developpement, Ecole Nationale d Agronomie de Montpellier, Cedex, France
Proc Natl Acad Sci U S A 97:14795-800. 2000..the effect of bacterial photosynthesis during symbiosis, we generated a photosynthesis-negative mutant of the Bradyrhizobium sp. strain ORS278 symbiont of Aeschynomene sensitiva...
Oxidation of elemental sulfur by Fusarium solani strain THIF01 harboring endobacterium Bradyrhizobium spXian Shu Li
Department of Material Science and Engineering, Nagoya Institute of Technology, Showa Ku, Nagoya, Japan
Microb Ecol 60:96-104. 2010..16S rRNA gene and analyses of full 16S rRNA gene sequence indicated strain THIF01 harbors an endobacterium Bradyrhizobium sp.; however, involvement of the bacterium in the sulfur oxidation is still unclear...
Phylogenetic analysis of the genera Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium on the basis of 16S rRNA gene and internally transcribed spacer region sequencesSoon Wo Kwon
Korean Agricultural Culture Collection KACC, Genetic Resources Division, National Institute of Agricultural Biotechnology, Rural Development Administration RDA, Suwon 441 707, Korea
Int J Syst Evol Microbiol 55:263-70. 2005..Phylogenetic relationships between these Korean isolates and reference strains of the genera Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium were analysed using their 16S rRNA gene and internally transcribed ..
Conflicting phylogeographic patterns in rRNA and nifD indicate regionally restricted gene transfer in BradyrhizobiumMatthew A Parker
Department of Biological Sciences, State University of New York, Binghamton, NY 13902, USA
Microbiology 148:2557-65. 2002..of the 16S rRNA gene and the nitrogenase alpha-subunit gene (nifD) were observed among 38 strains of Bradyrhizobium sp. nodule bacteria from North America, Central America, Asia and Australia...
Lack of galactose or galacturonic acid in Bradyrhizobium japonicum USDA 110 exopolysaccharide leads to different symbiotic responses in soybeanJuan Ignacio Quelas
Instituto de Biotecnologia y Biologia Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata y CCT La Plata CONICET, Calles 47 y 115 1900 La Plata, Argentina
Mol Plant Microbe Interact 23:1592-604. 2010Exopolysaccharide (EPS) and lipopolysaccharide (LPS) from Bradyrhizobium japonicum are important for infection and nodulation of soybean (Glycine max), although their roles are not completely understood...
Kinetic and thermodynamic analysis of Bradyrhizobium japonicum PutA-membrane associationsWeimin Zhang
Department of Biochemistry, Redox Biology Center, University of Nebraska, Lincoln, NE 68588, USA
Arch Biochem Biophys 445:174-83. 2006..In other gram-negative bacteria such as Bradyrhizobium japonicum, PutA lacks DNA binding activity and functions only as a proline catabolic enzyme...
Disparate pathways for the biogenesis of cytochrome oxidases in Bradyrhizobium japonicumDoris Bühler
Institute of Microbiology, Swiss Federal Institute of Technology, Wolfgang Pauli Strasse 10, CH 8093 Zurich, Switzerland
J Biol Chem 285:15704-13. 2010This work addresses the biogenesis of heme-copper terminal oxidases in Bradyrhizobium japonicum, the nitrogen-fixing root nodule symbiont of soybean. B. japonicum has four quinol oxidases and four cytochrome oxidases...
Phylogenetic analyses of symbiotic nodulation genes support vertical and lateral gene co-transfer within the Bradyrhizobium genusLionel Moulin
, IRD-INRA-CIRAD-ENSAM, 34398 Montpellier, France
Mol Phylogenet Evol 30:720-32. 2004..PCR amplification, sequencing and phylogenetic analysis of nodA gene sequences from a collection of diverse Bradyrhizobium strains revealed the monophyletic character with the possible exception of photosynthetic Bradyrhizobium, ..
A new nitrilase from Bradyrhizobium japonicum USDA 110. Gene cloning, biochemical characterization and substrate specificityDunming Zhu
Department of Chemistry, Southern Methodist University, Dallas, TX 75275, USA
J Biotechnol 133:327-33. 2008A nitrilase gene blr3397 from Bradyrhizobium japonicum USDA110 was cloned and over-expressed in Escherichia coli, and the encoded protein was purified to give a nitrilase with a single band of about 34.5kD on SDS-PAGE...
Rhizobial adaptation to hosts, a new facet in the legume root-nodule symbiosisMarion Koch
Institute of Microbiology, ETH, Zurich, Switzerland
Mol Plant Microbe Interact 23:784-90. 2010..The host-adaptation concept was elaborated with Bradyrhizobium japonicum and three different legumes (soybean, cowpea, and siratro)...
Discovery of a haem uptake system in the soil bacterium Bradyrhizobium japonicumA Nienaber
Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
Mol Microbiol 41:787-800. 2001In Bradyrhizobium japonicum, the nitrogen-fixing symbiont of soybeans, we have identified a haem uptake system, Hmu, that comprises a cluster of nine open reading frames...
Control of peripheral light-harvesting complex synthesis by a bacteriophytochrome in the aerobic photosynthetic bacterium Bradyrhizobium strain BTAi1Marianne Jaubert
Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD, CIRAD, AGRO M, INRA, UM2, TA A 82 J, Campus de Baillarguet, 34398 Montpellier Cedex 5, France
J Bacteriol 190:5824-31. 2008The recent sequence analysis of the photosynthetic and plant-symbiotic Bradyrhizobium sp. strain BTAi1 revealed the unexpected presence of a pucBA operon encoding the apoproteins of peripheral light-harvesting (LH) complexes...
In situ phylogenetic structure and diversity of wild Bradyrhizobium communitiesJ L Sachs
Department of Integrative Biology, University of California, Berkeley, California 94720, USA
Appl Environ Microbiol 75:4727-35. 2009..clustering and diversity were investigated in a natural community of rhizobial bacteria from the genus Bradyrhizobium. These bacteria live in the soil and also form beneficial root nodule symbioses with legumes, including those ..
Origins of cheating and loss of symbiosis in wild BradyrhizobiumJ L Sachs
Integrative Biology, University of California, Berkeley, CA, USA
J Evol Biol 23:1075-89. 2010..Phylogenetic reconstruction of Bradyrhizobium rDNA and transmissible symbiosis-island loci suggest that the cheater evolved via symbiotic gene transfer...
Divergent Bradyrhizobium symbionts on Tachigali versicolor from Barro Colorado Island, PanamaM A Parker
Department of Biological Sciences, State University of New York, Binghamton 13902 6000, USA
Syst Appl Microbiol 23:585-90. 2000..versicolor fall into the genus Bradyrhizobium. However, they are divergent from the bradyrhizobia associated with other BCI legumes, as well as from other ..
Large-scale transposon mutagenesis of photosynthetic Bradyrhizobium sp. strain ORS278 reveals new genetic loci putatively important for nod-independent symbiosis with Aeschynomene indicaKatia Bonaldi
Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD, UMR IRD SupAgro INRA UM2 CIRAD, F 34398 Montpellier, France
Mol Plant Microbe Interact 23:760-70. 2010Photosynthetic Bradyrhizobium strains possess the unusual ability to form nitrogen-fixing nodules on a specific group of legumes in the absence of Nod factors...
The Bradyrhizobium japonicum napEDABC genes encoding the periplasmic nitrate reductase are essential for nitrate respirationMaria J Delgado
Departamento de Microbiologia del Suelo y Sistemas Simbioticos, Estacion Experimental del Zaidin, CSIC, E 18080 Granada, Spain
Microbiology 149:3395-403. 2003The napEDABC gene cluster that encodes the periplasmic nitrate reductase from Bradyrhizobium japonicum USDA110 has been isolated and characterized...
Transcription profiling of soybean nodulation by Bradyrhizobium japonicumLaurent Brechenmacher
National Center for Soybean Biotechnology, Divisions of Plant Sciences and Biochemistry, University of Missouri, Columbia, MO 65211, USA
Mol Plant Microbe Interact 21:631-45. 2008..microarrays, we monitored gene expression in soybean (Glycine max) inoculated with the nodulating bacterium Bradyrhizobium japonicum 4, 8, and 16 days after inoculation, timepoints that coincide with nodule development and the onset ..
Population genetics and phylogenetic inference in bacterial molecular systematics: the roles of migration and recombination in Bradyrhizobium species cohesion and delineationPablo Vinuesa
Centro de Investigacion Sobre Fijacion de Nitrogeno, Programa de Ecología Molecular y Microbiana, UNAM, Av Universidad s n, Col Chamilpa, AP 565 A, Cuernavaca CP 62210, Morelos, Mexico
Mol Phylogenet Evol 34:29-54. 2005A combination of population genetics and phylogenetic inference methods was used to delineate Bradyrhizobium species and to uncover the evolutionary forces acting at the population-species interface of this bacterial genus...
A comparative proteomic evaluation of culture grown vs nodule isolated Bradyrhizobium japonicumAnnamraju D Sarma
Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA
Proteomics 6:3008-28. 2006Total protein extract of Bradyrhizobium japonicum cultivated in HM media were resolved by 2-D PAGE using narrow range IPG strips...
A two-component regulator mediates population-density-dependent expression of the Bradyrhizobium japonicum nodulation genesJohn Loh
Center for Legume Research and Department of Microbiology, The University of Tennessee, Knoxville, Tennessee 37996, USA
J Bacteriol 184:1759-66. 2002b>Bradyrhizobium japonicum nod gene expression was previously shown to be population density dependent. Induction of the nod genes is highest at low culture density and repressed at high population densities...
Identification of genistein-inducible and type III-secreted proteins of Bradyrhizobium japonicumChristin Süss
Institut fur Genetik, Technische Universitat Dresden, Helmholtzstrasse 10, 01069 Dresden, Germany
J Biotechnol 126:69-77. 2006Flagellin is the bulk protein secreted by Bradyrhizobium japonicum. For easier identification of minor protein fractions, the flagellin genes bll6865 and bll6866 were deleted...
The promoters of two isoflavone synthase genes respond differentially to nodulation and defense signals in transgenic soybean rootsSenthil Subramanian
Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA
Plant Mol Biol 54:623-39. 2004..b>Bradyrhizobium japonicum, a diazotropic symbiont of soybean, induced the expression of IFS1:GUS in root hairs and xylem poles ..
An oligonucleotide microarray resource for transcriptional profiling of Bradyrhizobium japonicumWoo Suk Chang
National Center for Soybean Biotechnology, University of Missouri, Columbia 65211, USA
Mol Plant Microbe Interact 20:1298-307. 2007..70-mer oligonucleotides, representing 8,453 open reading frames (ORFs), was constructed based on the Bradyrhizobium japonicum strain USDA110 genomic sequence...
Insights learned from pBTAi1, a 229-kb accessory plasmid from Bradyrhizobium sp. strain BTAi1 and prevalence of accessory plasmids in other Bradyrhizobium sp. strainsEddie J Cytryn
Department of Soil, Water, and Climate, Biotechnology Institute, University of Minnesota, St Paul, MN 55108, USA
ISME J 2:158-70. 2008..silico, physiological and in planta analyses were used to characterize pBTAi1, a 229-kb accessory plasmid from Bradyrhizobium sp...
Flavonoid-responsive nodY-lacZ expression in three phylogenetically different Bradyrhizobium groupsTadashi Yokoyama
Tokyo University of Agriculture and Technology, Saiwai cho 3 5 8, Fuchu, Tokyo 183 8509, Japan
Can J Microbiol 54:401-10. 2008..analysis using the nodD1YABC gene probe showed the genetic diversity of common nodD1ABC gene regions of Bradyrhizobium japonicum, Bradyrhizobium elkanii, and the Thai soybean Bradyrhizobium...
Cowpea and peanut in southern Africa are nodulated by diverse Bradyrhizobium strains harboring nodulation genes that belong to the large pantropical clade common in AfricaEmma T Steenkamp
Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0002, South Africa
Mol Phylogenet Evol 48:1131-44. 2008..unguiculata) and peanut (Arachis hypogaea) in southern Africa are nodulated by a genetically diverse group of Bradyrhizobium strains...
Characterization of two sets of subpolar flagella in Bradyrhizobium japonicumMasaomi Kanbe
Department of Life Sciences, Prefectural University of Hiroshima, 562 Nanatsuka, Shobara, Hiroshima 727 0023, Japan
J Bacteriol 189:1083-9. 2007b>Bradyrhizobium japonicum is one of the soil bacteria that form nodules on soybean roots. The cell has two sets of flagellar systems, one thick flagellum and a few thin flagella, uniquely growing at subpolar positions...
Expression of the Bradyrhizobium japonicum type III secretion system in legume nodules and analysis of the associated tts box promoterSusanne Zehner
Institute of Genetics, Dresden University of Technology, Helmholtzstrasse 10, Dresden, Germany
Mol Plant Microbe Interact 21:1087-93. 2008In Bradyrhizobium japonicum, as in some other rhizobia, symbiotic efficiency is influenced by a type III secretion system (T3SS)...
Nodulation gene regulation in Bradyrhizobium japonicum: a unique integration of global regulatory circuitsJohn Loh
Department of Plant Microbiology and Pathology, University of Missouri, Columbia, Missouri 65211, USA
Appl Environ Microbiol 69:10-7. 2003
Comprehensive assessment of the regulons controlled by the FixLJ-FixK2-FixK1 cascade in Bradyrhizobium japonicumSocorro Mesa
Institute of Microbiology, ETH, Wolfgang Pauli Strasse 10, CH 8093 Zurich, Switzerland
J Bacteriol 190:6568-79. 2008Symbiotic N(2) fixation in Bradyrhizobium japonicum is controlled by a complex transcription factor network...
Mutational and transcriptional analysis of the type III secretion system of Bradyrhizobium japonicumAndrea Krause
Institut fur Genetik, Technische Universitat Dresden, Mommsenstrasse 13, 01062 Dresden, Germany
Mol Plant Microbe Interact 15:1228-35. 2002Sequencing the symbiotic region of Bradyrhizobium japonicum revealed a gene cluster (tts) encoding a type III secretion system (TTSS) that is similar to those found in Mesorhizobium loti MAFF303099 and Rhizobium strain NGR234...
Conservation of type III secretion system genes in Bradyrhizobium isolated from soybeanSylvie Mazurier
INRA Université de Bourgogne, UMR Microbiologie et Géochimie des Sols, CMSE, Dijon, France
FEMS Microbiol Lett 259:317-25. 2006The distribution of rhcRST genes encoding the type III secretion system (T3SS) in a collection of Bradyrhizobium strains was characterized by PCR and Southern blot hybridization...
Investigation of downstream signals of the soybean autoregulation of nodulation receptor kinase GmNARKMark Kinkema
The University of Queensland, St Lucia, QLD 4072, Australia
Mol Plant Microbe Interact 21:1337-48. 2008..biosynthesis as well as JA-response genes were regulated systemically but not locally by root inoculation with Bradyrhizobium japonicum...
Functional role of Bradyrhizobium japonicum trehalose biosynthesis and metabolism genes during physiological stress and nodulationMasayuki Sugawara
Department of Soil, Water, and Climate, University of Minnesota, St Paul, MN 55108, USA
Appl Environ Microbiol 76:1071-81. 2010..Previous studies reported that the trehalose biosynthetic genes (otsA, treS, and treY) in Bradyrhizobium japonicum were induced by salinity and desiccation stresses...
Perception of Bradyrhizobium japonicum Nod factor by soybean [Glycine max (L.) Merr.] root hairs under abiotic stress conditionsH M Duzan
Department of Plant Science, McGill University, Macdonald Campus, 21111 Lakeshore Road, Ste. Anne de Bellevue, Quebec, Canada H9X 3V9
J Exp Bot 55:2641-6. 2004..Early stages of the Bradyrhizobium japonicum-soybean [Glycine max (L.) Merr...
Transcriptional and physiological responses of Bradyrhizobium japonicum to desiccation-induced stressEddie J Cytryn
Department of Soil, Water, and Climate, Biotechnology Institute, University of Minnesota, St Paul, Minnesota, USA corrected
J Bacteriol 189:6751-62. 2007..study, we used genome-wide transcriptional analyses to obtain a comprehensive understanding of the response of Bradyrhizobium japonicum to drought...
Dissection of the Bradyrhizobium japonicum NifA+sigma54 regulon, and identification of a ferredoxin gene (fdxN) for symbiotic nitrogen fixationFelix Hauser
Institute of Microbiology, Eidgenossische Technische Hochschule, Wolfgang Pauli Strasse 10, 8093 Zurich, Switzerland
Mol Genet Genomics 278:255-71. 2007..network for expression control of symbiotic and accessory genes in the nitrogen-fixing soybean symbiont Bradyrhizobium japonicum...
The hmuQ and hmuD genes from Bradyrhizobium japonicum encode heme-degrading enzymesSumant Puri
Department of Biochemistry, 140 Farber Hall, State University of New York at Buffalo, Buffalo, NY 14214, USA
J Bacteriol 188:6476-82. 2006..b>Bradyrhizobium japonicum can use heme as an iron source, but no heme-degrading oxygenase has been described...
Oxidative stress promotes degradation of the Irr protein to regulate haem biosynthesis in Bradyrhizobium japonicumJianhua Yang
Department of Biochemistry and Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, NY 14214, USA
Mol Microbiol 60:209-18. 2006..In the bacterium Bradyrhizobium japonicum, expression of the gene encoding the haem biosynthesis enzyme delta-aminolevulinic acid dehydratase (..
Endogenous isoflavones are essential for the establishment of symbiosis between soybean and Bradyrhizobium japonicumSenthil Subramanian
Donald Danforth Plant Science Center, St Louis, MO 63132, USA
Plant J 48:261-73. 2006..are essential for nodulation of soybean roots because of their ability to induce the nodulation genes of Bradyrhizobium japonicum...
The Iron control element, acting in positive and negative control of iron-regulated Bradyrhizobium japonicum genes, is a target for the Irr proteinGesine Rudolph
Institute of Microbiology, , Wolfgang-Pauli-Strasse 10, , Switzerland
J Bacteriol 188:733-44. 2006b>Bradyrhizobium japonicum, the nitrogen-fixing soybean symbiont, possesses a heme uptake system encoded by the gene cluster hmuVUT-hmuR-exbBD-tonB...
Effect of two AMF life strategies on the tripartite symbiosis with Bradyrhizobium japonicum and soybeanPedro M Antunes
Department of Land Resource Science, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
Mycorrhiza 16:167-73. 2006..mycorrhizal fungi (AMF) with different life-history strategies to the tripartite symbiosis with soybeans and Bradyrhizobium japonicum (Kirchner) Jordan...
Jasmonates induce Nod factor production by Bradyrhizobium japonicumF Mabood
Plant Science Department, Macdonald Campus of McGill University, 21, 111 Lakeshore Road, Sainte Anne de Bellevue, Que, Canada
Plant Physiol Biochem 44:759-65. 2006..We have previously demonstrated that jasmonates can induce nod genes of Bradyrhizobium japonicum when measured by beta-galactosidase activity...
Transcription activation in vitro by the Bradyrhizobium japonicum regulatory protein FixK2Socorro Mesa
Institute of Microbiology, Eidgenossische Technische Hochschule, CH 8093 Zurich, Switzerland
J Bacteriol 187:3329-38. 2005In Bradyrhizobium japonicum, the N2-fixing root nodule endosymbiont of soybean, a group of genes required for microaerobic, anaerobic, or symbiotic growth is controlled by FixK2, a key regulator that is part of the FixLJ-FixK2 cascade...
Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and BradyPablo Vinuesa
Centro de Ciencias Genomicas, UNAM, Av Universidad s n, Col Chamilpa, CP 62210, AP 565 A, Cuernavaca, Morelos, Mexico
Int J Syst Evol Microbiol 55:569-75. 2005Highly diverse Bradyrhizobium strains nodulate genistoid legumes (brooms) in the Canary Islands, Morocco, Spain and the Americas...
Novel avidin-like protein from a root nodule symbiotic bacterium, Bradyrhizobium japonicumHenri R Nordlund
Department of Biological and Environmental Science, Nanoscience Center, P. O. Box 35 (YAB, , Finland
J Biol Chem 280:13250-5. 2005b>Bradyrhizobium japonicum is an important nitrogenfixing symbiotic bacterium, which can form root nodules on soybeans...
Rhizobitoxine production in Agrobacterium tumefaciens C58 by Bradyrhizobium elkanii rtxACDEFG genesMasayuki Sugawara
Graduate School of Life Sciences, Tohoku University, Katahira, Aoba ku, Sendai, Japan
FEMS Microbiol Lett 269:29-35. 2007..for production of rhizobitoxine, an inhibitor of ethylene biosynthesis in plants, directed by the rtx genes of Bradyrhizobium elkanii...
Two heme binding sites are involved in the regulated degradation of the bacterial iron response regulator (Irr) proteinJianhua Yang
Department of Biochemistry, State University of New York, Buffalo, New York 14214, USA
J Biol Chem 280:7671-6. 2005The iron response regulator (Irr) protein from Bradyrhizobium japonicum is a conditionally stable protein that degrades in response to cellular iron availability...
Evidence of horizontal transfer of symbiotic genes from a Bradyrhizobium japonicum inoculant strain to indigenous diazotrophs Sinorhizobium (Ensifer) fredii and Bradyrhizobium elkanii in a Brazilian Savannah soilFernando Gomes Barcellos
Embrapa Soja, Cx Postal 231, 86001 970 Londrina, Parana, Brazil
Appl Environ Microbiol 73:2635-43. 2007..Both soybean (Glycine max [L.] Merrill) and compatible Bradyrhizobium japonicum and Bradyrhizobium elkanii strains are exotic to Brazil and have been massively introduced in the ..
Legumes symbioses: absence of Nod genes in photosynthetic bradyrhizobiaEric Giraud
Institut de Recherche pour le Developpement, Centre de Coopération International en Recherche Agronomique pour le Développement, Institut National de la Recherche Agronomique, Universite Montpellier 2, France
Science 316:1307-12. 2007..Here we show by complete genome sequencing of two symbiotic, photosynthetic, Bradyrhizobium strains, BTAi1 and ORS278, that canonical nodABC genes and typical lipochito-oligosaccharidic Nod factors are ..
Effect of trehalose on survival of Bradyrhizobium japonicum during desiccationJ G Streeter
Department of Horticulture and Crop Science, Ohio State University OARDC, Wooster, OH 44691, USA
J Appl Microbiol 95:484-91. 2003..The major purpose of this study was to identify conditions under which alpha,alpha-trehalose would improve survival of Bradyrhizobium japonicum during desiccation.
Small-subunit rRNA genotyping of rhizobia nodulating Australian Acacia sppB Lafay
Centre for Plant Biodiversity Research, CSIRO Plant Industry, Canberra ACT 2601, Australia
Appl Environ Microbiol 67:396-402. 2001..J. Burdon, Appl. Environ. Microbiol. 64:3989-3997, 1998). Eight of these genomospecies belonged to the Bradyrhizobium lineage and accounted for 96.6% of the isolates. The remaining genomospecies corresponded to Rhizobium tropici...
Stimulation of adhesiveness, infectivity, and competitiveness for nodulation of Bradyrhizobium japonicum by its pretreatment with soybean seed lectinA R Lodeiro
Instituto de Bioquimica y Biologia Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata UNLP, Calles 47 y 115 1900, La Plata, Argentina
FEMS Microbiol Lett 188:177-84. 2000Soybean seed lectin stimulates adsorption of Bradyrhizobium japonicum to its host roots...
Two genes encoding a putative multidrug efflux pump of the RND/MFP family are cotranscribed with an rpoH gene in Bradyrhizobium japonicumP Krummenacher
Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Zurich, Switzerland
Gene 241:247-54. 2000The rpoH3 gene of Bradyrhizobium japonicum codes for one of three sigma32-type transcription factors in this organism and is flanked by rag (rpoH3-associated) genes comprising the chromosomal arrangement ragABrpoH3ragCD...
Potential symbiosis-specific genes uncovered by sequencing a 410-kilobase DNA region of the Bradyrhizobium japonicum chromosomeM Gottfert
Institut fur Genetik, Technische Universitat Dresden, D 01062 Dresden, Germany
J Bacteriol 183:1405-12. 2001The physical and genetic map of the Bradyrhizobium japonicum chromosome revealed that nitrogen fixation and nodulation genes are clustered...
Structure of the soluble domain of a membrane-anchored thioredoxin-like protein from Bradyrhizobium japonicum reveals unusual propertiesG Capitani
Biochemisches Institut, Universitat Zurich, CH 8057 Zurich, Switzerland
J Mol Biol 311:1037-48. 2001TlpA is an unusual thioredoxin-like protein present in the nitrogen-fixing soil bacterium Bradyrhizobium japonicum...
Symbiotic hydrogenase activity in Bradyrhizobium sp. (Vigna) increases nitrogen content in Vigna unguiculata plantsCecilia Baginsky
Laboratory of Microbiology, Department of Biotechnology, , , Ciudad Universitaria s/n, 28040 Madrid, Spain
Appl Environ Microbiol 71:7536-8. 2005b>Bradyrhizobium sp. (Lupinus) and Bradyrhizobium sp. (Vigna) mutants in which hydrogenase (hup) activity was affected were constructed and analyzed. Vigna unguiculata plants inoculated with the Bradyrhizobium sp...
Global protein expression pattern of Bradyrhizobium japonicum bacteroids: a prelude to functional proteomicsAnnamraju D Sarma
Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA
Proteomics 5:4170-84. 2005..understanding the process of symbiotic nitrogen fixation between the legume soybean and the soil bacteria Bradyrhizobium japonicum, we examined the total protein expression pattern of the nodule bacteria, often referred to as ..
An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosisHeather P Benson
Department of Biological Sciences, 6044 Gilman, Dartmouth College, Hanover, NH 03755, USA
Mol Plant Microbe Interact 18:950-9. 2005..Each environment presents different challenges with respect to iron acquisition. The soybean symbiont Bradyrhizobium japonicum 61A152 can utilize a variety of siderophores (Fe[III]-specific ligands)...
Diverse rhizobia associated with woody legumes Wisteria sinensis, Cercis racemosa and Amorpha fruticosa grown in the temperate zone of ChinaJie Liu
Key Laboratory of Agro Microbial Resource and Application, Ministry of Agriculture Department of Microbiology, College of Biological Sciences, China Agricultural University, 100094 Beijing, PR China
Syst Appl Microbiol 28:465-77. 2005..were defined in numerical taxonomy, which corresponded to distinct groups within the genera Agrobacterium, Bradyrhizobium, Mesorhizobium and Rhizobium in 16S and 23S rRNA gene PCR-RFLP...
Diverse bacteria isolated from root nodules of Phaseolus vulgaris and species within the genera Campylotropis and Cassia grown in ChinaSu-Zhen Han
Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, China
Syst Appl Microbiol 28:265-76. 2005..four species of Cassia grown in the central zones of China were classified into three groups within the genus Bradyrhizobium. One of these three groups could be defined as Bradyrhizobium japonicum. Our results demonstrated that P...
SNP identification and SNAP marker development for a GmNARK gene controlling supernodulation in soybeanM Y Kim
Department of Plant Science, Seoul National University, San 56-1, Shillim-dong, Kwanak-Gu, Seoul, 151-921, The Republic of Korea
Theor Appl Genet 110:1003-10. 2005..Therefore, use of this SNAP marker might be faster, cheaper, and more reproducible than using other genotyping methods, such as a cleaved amplified polymorphic sequence marker, which demand of restriction enzymes...
Prevalence of Burkholderia sp. nodule symbionts on four mimosoid legumes from Barro Colorado Island, PanamaCraig F Barrett
Department of Biological Sciences, State University of New York, Binghamton, NY 13902-6000, USA
Syst Appl Microbiol 28:57-65. 2005..hymenaeafolium), 44 were Burkholderia strains while the rest were placed in Rhizobium, Mesorhizobium, or Bradyrhizobium. The Burkholderia strains displayed four distinct rRNA sequence types, ranging from 89% to 97% similarity for ..
Ectomycorrhizal symbiosis enhanced the efficiency of inoculation with two Bradyrhizobium strains and Acacia holosericea growthS Andre
UMR 113 CIRAD INRA IRD AGRO M UM2, Laboratoire des Symbioses Tropicales et Méditerranéennes, TA10 J, Campus International de Baillarguet, 34398 Montpellier, France
Mycorrhiza 15:357-64. 2005Two strains of Bradyrhizobium sp...
Symbiotic Bradyrhizobium japonicum reduces N2O surrounding the soybean root system via nitrous oxide reductaseReiko Sameshima-Saito
Graduate School of Life Sciences, Tohoku University, Katahira, Aoba ku, Sendai 980 8577, Japan
Appl Environ Microbiol 72:2526-32. 2006N(2)O reductase activity in soybean nodules formed with Bradyrhizobium japonicum was evaluated from N(2)O uptake and conversion of (15)N-N(2)O into (15)N-N(2)...
Effect of organic N source on bacterial growth, lipo-chitooligosaccharide production, and early soybean nodulation by Bradyrhizobium japonicumS Supanjani
Department of Plant Science, McGill University, Macdonald Campus, Canada
Can J Microbiol 52:227-36. 2006Production of Bradyrhizobium japonicum inoculants is problematic because high inoculation rates are necessary but expensive, while production of rhizobial Nod factors (lipo-chitooligosaccharides (LCOs)), key signal molecules in the ..
Bradyrhizobia isolated from root nodules of Parasponia (Ulmaceae) do not constitute a separate coherent lineageBenedicte Lafay
CSIRO Plant Industry, PO Box 1600, Canberra, ACT 2601, Australia
Int J Syst Evol Microbiol 56:1013-8. 2006..on the 16S rRNA or nodA gene sequences, but were scattered among other bradyrhizobia belonging to either the Bradyrhizobium japonicum or the Bradyrhizobium elkanii lineages...
Bradyrhizobium elkanii, Bradyrhizobium yuanmingense and Bradyrhizobium japonicum are the main rhizobia associated with Vigna unguiculata and Vigna radiata in the subtropical region of ChinaYong Fa Zhang
The Key Laboratory of Agro Microbial Resource and Application Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China
FEMS Microbiol Lett 285:146-54. 2008..The results showed that 90% of the analyzed strains belonged to or were related to Bradyrhizobium japonicum, Bradyrhizobium liaoningense, Bradyrhizobium yuanmingense and Bradyrhizobium elkanii, while the ..
Soybean seed extracts preferentially express genomic loci of Bradyrhizobium japonicum in the initial interaction with soybean, Glycine max (L.) MerrMin Wei
Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
DNA Res 15:201-14. 2008..In this study, the global expression profiles of Bradyrhizobium japonicum USDA 110 in response to soybean (Glycine max) seed extracts (SSE) and genistein, a major soybean-..
The genistein stimulon of Bradyrhizobium japonicumKathrin Lang
Institute of Genetics, Dresden University of Technology, Helmholtzstrasse 10, 01069, Dresden, Germany
Mol Genet Genomics 279:203-11. 2008..Here we report the genome-wide transcriptional response of Bradyrhizobium japonicum to genistein, an isoflavone secreted by soybean. About 100 genes were induced in the wild type...
Plant science. Infectious heresyJ Allan Downie
Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK
Science 316:1296-7. 2007
Diverse bacteria isolated from root nodules of Trifolium, Crotalaria and Mimosa grown in the subtropical regions of ChinaXiao Yun Liu
Key Laboratory of Agro Microbial Resource and Application, Ministry of Agriculture College of Biological Sciences, China Agricultural University, Beijing, 100094, China
Arch Microbiol 188:1-14. 2007..proteins, sequencing of 16S rDNA and DNA-DNA hybridization grouped the isolates into 17 lineages belonging to Bradyrhizobium, Mesorhizobium, Rhizobium, Sinorhizobium and Burkholderia, as well as a non-symbiotic group of Agrobacterium...
Nodulation gene regulation and quorum sensing control density-dependent suppression and restriction of nodulation in the Bradyrhizobium japonicum-soybean symbiosisSiriluck Jitacksorn
University of Minnesota, Department of Soil, Water, and Climate, University of Minnesota, St Paul, MN 55108, USA
Appl Environ Microbiol 74:3749-56. 2008..Lambert and the nodulation-restricting plant introduction (PI) genotype PI 417566 by wild-type Bradyrhizobium japonicum USDA110 is regulated in a population-density-dependent manner...
[Glutamate dehydrogenase activity of Bradyrhizobium japonicum in the presence of phytoregulators]N O Leonova
Mikrobiol Z 68:20-6. 2006..It was shown that all used phytoregulators stimulated glutamate dehydrogenase activity of Bradyrhizobium japonicum 71t (the strain with highly efficient symbiotic properties) 1.2-4.9 times...
Isolation of a novel nodulin: a molecular marker of osmotic stress in Glycine max/Bradyrhizobium japonicum noduleMathilde Clement
, Institut Sophia Agrobiotech, 400 Routes des Chappes, 06903 Sophia-Antipolis Cedex, France
Plant Cell Environ 29:1841-52. 2006..The symbiosis between soybean (Glycine max) and Bradyrhizobium japonicum, because of its extreme sensitivity to drought, was chosen as a model to analyse the response to ..
Differential symbiotic response of phage-typed strains of Bradyrhizobium japonicum with soybean cultivarsChinnaswamy Appunu
Microbial Genetics Laboratory, Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India
J Microbiol 44:363-8. 2006In this study, native Bradyrhizobium strains were isolated from the host plant, Glycine max, harvested from fields in Madhya Pradesh, India, and were typed by lytic rhizobiophages...
Phylogeny of nodulation genes and symbiotic properties of Genista tinctoria bradyrhizobiaMichał Kalita
Department of General Microbiology, M Curie Skłodowska University, 19 Akademicka St, 20 033 Lublin, Poland
Arch Microbiol 186:87-97. 2006..sequences to those available in databanks revealed their highest sequence identities to nodulation loci of Bradyrhizobium sp. (Lupinus) strains and rhizobia from other genistoid legumes...
Diversity among Bradyrhizobium isolates nodulating yardlong bean and sunnhemp in GuamZ You
Department of Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, HI 96822, USA
J Appl Microbiol 93:577-84. 2002..that nodulate yardlong bean and sunnhemp in Guam were examined for genetic diversity and their relatedness to Bradyrhizobium japonicum and B. elkanii reference strains. Genomic DNA of 58 isolates of Bradyrhizobium spp...
Genetic diversity of native bradyrhizobia isolated from soybeans (Glycine max L.) in different agricultural-ecological-climatic regions of IndiaChinnaswamy Appunu
Microbial Genetics Laboratory, Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India
Appl Environ Microbiol 74:5991-6. 2008..Eight haplotypes assigned to the Bradyrhizobium genus were identified, and the genetic diversity was conserved across regions...
[The significance of exometabolites in the formation and operation of the soybean-rhizobium symbiosis]E V Kirichenko
Prikl Biokhim Mikrobiol 40:567-70. 2004The effect of various inoculates of the soybean-specific strain of nodule bacteria Bradyrhizobium japonicum 634b (unwashed cells, cells washed from the exopolysaccharide-protein complex, and cells combined with the complex) on the ..
A novel genetic locus outside the symbiotic island is required for effective symbiosis of Bradyrhizobium japonicum with soybean Glycine maxBernd Ulrich Becker
Philipps University of Marburg, Faculty of Biology, Cell Biology and Applied Botany, Karl von Frisch Str 8, 35032 Marburg, Germany
Res Microbiol 155:770-80. 2004In order to investigate the symbiotic interaction between soybean and Bradyrhizobium japonicum, TnphoA mutagenesis of the microsymbiont was performed. Mutant strain 2-10 was found to induce a strongly reduced number of ineffective nodules...
Research Grants
- STRUCTURE AND DYNAMICS OF HEME PROTEIN ACTIVE SITESJAMES SATTERLEE; Fiscal Year: 2005..These include the FixLs from Bradyrhizobium japonicum (BjFixL) and Sinorhizobium meliloti (SmFixL) and the Direct Oxygen Sensor protein from E...
- STRUCTURE AND DYNAMICS OF HEME PROTEIN ACTIVE SITESJAMES SATTERLEE; Fiscal Year: 2000....
- STUDIES OF THE DYNAMICS OF HEME PROTEIN ACTIVE SITESJAMES SATTERLEE; Fiscal Year: 1993..In addition we intend to use these results and others in order to test our ability to design and create mutants designed for a specific purpose, based upon inferences from the crystal structure...
- Regulation of Bacterial Heme MetabolismMARK R O apos BRIAN; Fiscal Year: 2010..We discovered a regulatory protein called Irr (iron response regulator) from the bacterium Bradyrhizobium japonicum that interacts directly with ferrochelatase...
- Regulation of Bacterial Heme BiosynthesisMARK O BRIAN; Fiscal Year: 2007..The Irr protein from the bacterium Bradyrhizobium japonicum represses the pathway at an early step under iron limitation to prevent protoporphyrin synthesis ..
- 2004 Tetrapyrroles Gordon ConferenceMARK O BRIAN; Fiscal Year: 2004..Strengths of this Gordon conference are its disciplinary diversity, focus on new unpublished work, time for discussion, informal atmosphere and ban on dissemination of conference proceedings. ..
- Regulation of Bacterial Heme MetabolismMARK R O apos BRIAN; Fiscal Year: 2011..We discovered a regulatory protein called Irr (iron response regulator) from the bacterium Bradyrhizobium japonicum that interacts directly with ferrochelatase...
