anaplasma marginale

Summary

Summary: A species of gram-negative bacteria and causative agent of severe bovine ANAPLASMOSIS. It is the most pathogenic of the ANAPLASMA species.

Top Publications

  1. doi The natural history of Anaplasma marginale
    Katherine M Kocan
    Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK 74078 2007, USA
    Vet Parasitol 167:95-107. 2010
  2. doi Targeting the tick protective antigen subolesin reduces vector infestations and pathogen infection by Anaplasma marginale and Babesia bigemina
    Octavio Merino
    Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ciudad Real, Spain
    Vaccine 29:8575-9. 2011
  3. ncbi Reduction of tick infections with Anaplasma marginale and A. phagocytophilum by targeting the tick protective antigen subolesin
    Jose De La Fuente
    Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA
    Parasitol Res 100:85-91. 2006
  4. pmc Transformation of Anaplasma marginale
    Roderick F Felsheim
    Department of Entomology, University of Minnesota, St Paul, MN 55108, USA
    Vet Parasitol 167:167-74. 2010
  5. pmc Quantitative differences in salivary pathogen load during tick transmission underlie strain-specific variation in transmission efficiency of Anaplasma marginale
    Massaro W Ueti
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 77:70-5. 2009
  6. pmc Multistrain genome analysis identifies candidate vaccine antigens of Anaplasma marginale
    Michael J Dark
    Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA
    Vaccine 29:4923-32. 2011
  7. doi A review of bovine anaplasmosis
    P Aubry
    Canadian Food Inspection Agency, Ottawa, ON, Canada
    Transbound Emerg Dis 58:1-30. 2011
  8. ncbi Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale
    Flábio R Araújo
    Embrapa Gado de Corte, Rodovia BR 262, 79002 970 Campo Grande, MS, Brazil
    Mem Inst Oswaldo Cruz 100:765-9. 2005
  9. pmc Functional epitope core motif of the Anaplasma marginale major surface protein 1a and its incorporation onto bioelectrodes for antibody detection
    Paula S Santos
    Laboratório de Nanobiotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de Uberlandia, Uberlandia, Brazil
    PLoS ONE 7:e33045. 2012
  10. pmc Experimental transmission of Anaplasma marginale by male Dermacentor reticulatus
    Zorica Zivkovic
    Utrecht Centre for Tick borne Diseases UCTD, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584CL, Utrecht, The Netherlands
    BMC Vet Res 3:32. 2007

Research Grants

  1. Antigenic variation of Anaplasma marginale
    PATRICK MEEUS; Fiscal Year: 2003
  2. MOLECULAR MECHANISMS OF MSP2 VARIATION IN RICKETTSIAE
    Anthony Barbet; Fiscal Year: 2007
  3. MOLECULAR MECHANISMS OF MSP2 VARIATION IN RICKETTSIAE
    Anthony Barbet; Fiscal Year: 2003
  4. MOLECULAR MECHANISMS OF MSP2 VARIATION IN RICKETTSIAE
    Anthony Barbet; Fiscal Year: 2006
  5. MOLECULAR MECHANISMS OF MSP2 VARIATION IN RICKETTSIAE
    Anthony Barbet; Fiscal Year: 2002
  6. Immunity directed against MSP 2 during anaplasmosis
    Jeffrey Abbott; Fiscal Year: 2004
  7. Immunogenicity of the Type IV Secretin System
    WENDY CATHERINE BROWN; Fiscal Year: 2010
  8. Identification of T-Cell Immunogens in Anaplasma
    Wendy Brown; Fiscal Year: 2007
  9. Immunogenicity of the Type IV Secretin System
    Wendy Brown; Fiscal Year: 2009
  10. IMMUNOLOGY TRAINING PROGRAM
    Guy Palmer; Fiscal Year: 2007

Detail Information

Publications199 found, 100 shown here

  1. doi The natural history of Anaplasma marginale
    Katherine M Kocan
    Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK 74078 2007, USA
    Vet Parasitol 167:95-107. 2010
    The intracellular pathogen Anaplasma marginale (Rickettsiales: Anaplasmataceae), described by Sir Arnold Theiler in 1910, is endemic worldwide in tropical and subtropical areas. Infection of cattle with A...
  2. doi Targeting the tick protective antigen subolesin reduces vector infestations and pathogen infection by Anaplasma marginale and Babesia bigemina
    Octavio Merino
    Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ciudad Real, Spain
    Vaccine 29:8575-9. 2011
    ..In this study, we used the fact that these animals were naturally infected with Anaplasma marginale and Babesia bigemina to evaluate the effect of subolesin vaccination and gene knockdown on tick infection ..
  3. ncbi Reduction of tick infections with Anaplasma marginale and A. phagocytophilum by targeting the tick protective antigen subolesin
    Jose De La Fuente
    Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA
    Parasitol Res 100:85-91. 2006
    ..RNAi or vaccination interfered with the ability of ticks to become infected with two tick-borne pathogens, Anaplasma marginale which causes bovine anaplasmosis and Anaplasma phagocytophilum, the causative agent of human granulocytin ..
  4. pmc Transformation of Anaplasma marginale
    Roderick F Felsheim
    Department of Entomology, University of Minnesota, St Paul, MN 55108, USA
    Vet Parasitol 167:167-74. 2010
    The tick-borne pathogen, Anaplasma marginale, has a complex life cycle involving ruminants and ixodid ticks. It causes bovine anaplasmosis, a disease with significant economic impact on cattle farming worldwide...
  5. pmc Quantitative differences in salivary pathogen load during tick transmission underlie strain-specific variation in transmission efficiency of Anaplasma marginale
    Massaro W Ueti
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 77:70-5. 2009
    ..Strains of the tick-borne rickettsia Anaplasma marginale differ markedly in transmission efficiency, with a consequent impact on pathogen strain structure...
  6. pmc Multistrain genome analysis identifies candidate vaccine antigens of Anaplasma marginale
    Michael J Dark
    Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA
    Vaccine 29:4923-32. 2011
    ..Vaccines have been developed and marketed against the causative organism, Anaplasma marginale; however, these have not been widely used because of breakthrough infections caused by heterologous strains ..
  7. doi A review of bovine anaplasmosis
    P Aubry
    Canadian Food Inspection Agency, Ottawa, ON, Canada
    Transbound Emerg Dis 58:1-30. 2011
    Bovine anaplasmosis, caused by Anaplasma marginale, is an infectious but non-contagious disease...
  8. ncbi Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale
    Flábio R Araújo
    Embrapa Gado de Corte, Rodovia BR 262, 79002 970 Campo Grande, MS, Brazil
    Mem Inst Oswaldo Cruz 100:765-9. 2005
    ..enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle...
  9. pmc Functional epitope core motif of the Anaplasma marginale major surface protein 1a and its incorporation onto bioelectrodes for antibody detection
    Paula S Santos
    Laboratório de Nanobiotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de Uberlandia, Uberlandia, Brazil
    PLoS ONE 7:e33045. 2012
    Anaplasmosis, a persistent intraerythrocytic infection of cattle by Anaplasma marginale, causes severe anemia and a higher rate of abortion, resulting in significant loss to both dairy and beef industries...
  10. pmc Experimental transmission of Anaplasma marginale by male Dermacentor reticulatus
    Zorica Zivkovic
    Utrecht Centre for Tick borne Diseases UCTD, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584CL, Utrecht, The Netherlands
    BMC Vet Res 3:32. 2007
    ..anaplasmosis has been reported in several European countries, but the vector competency of tick species for Anaplasma marginale from these localities has not been determined...
  11. doi Epidemiological analysis of tick-borne diseases in Zambia
    Martin Simuunza
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden Road, Glasgow G61 1QH, Scotland, UK
    Vet Parasitol 175:331-42. 2011
    ..bovis), anaplasmosis (caused by Anaplasma marginale) and heartwater (caused by Ehrlichia ruminantium)...
  12. ncbi Intrahaplotype and interhaplotype pairing of bovine leukocyte antigen DQA and DQB molecules generate functional DQ molecules important for priming CD4(+) T-lymphocyte responses
    Junzo Norimine
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Immunogenetics 57:750-62. 2005
    ..and six DQ pairs for antigen-specific CD4(+) T-cell responses against tick-transmitted bovine hemoparasites Anaplasma marginale or Babesia bovis. Interhaplotype pairing of DQ A and B molecules was demonstrated...
  13. ncbi Relative efficiency of biological transmission of Anaplasma marginale (Rickettsiales: Anaplasmataceae) by Dermacentor andersoni (Acari: Ixodidae) compared with mechanical transmission by Stomoxys calcitrans (Diptera: Muscidae)
    Glen A Scoles
    USDA ARS, Animal Disease Research Unit, Washington State University, Pullman, WA 99164, USA
    J Med Entomol 42:668-75. 2005
    b>Anaplasma marginale Theiler is a tick-borne intraerythrocytic rickettsial pathogen of cattle that also can be mechanically transmitted by biting flies...
  14. pmc Identification of midgut and salivary glands as specific and distinct barriers to efficient tick-borne transmission of Anaplasma marginale
    Massaro W Ueti
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 75:2959-64. 2007
    ..Using the highly transmissible St. Maries strain of Anaplasma marginale in Dermacentor andersoni as a positive control and two unrelated nontransmissible strains, we identified ..
  15. pmc Stability and tick transmission phenotype of gfp-transformed Anaplasma marginale through a complete in vivo infection cycle
    Susan M Noh
    Animal Disease Research Unit, Agriculture Research Service, US Department of Agriculture, Pullman, WA 99164 7030, USA
    Appl Environ Microbiol 77:330-4. 2011
    We tested the stability and tick transmission phenotype of transformed Anaplasma marginale through a complete in vivo infection cycle. Similar to the wild type, the gfp-transformed A...
  16. pmc Silencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor variabilis
    Katherine M Kocan
    Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
    BMC Dev Biol 9:42. 2009
    The cattle pathogen, Anaplasma marginale, undergoes a developmental cycle in ticks that begins in gut cells. Transmission to cattle occurs from salivary glands during a second tick feeding. At each site of development two forms of A...
  17. doi Molecular identification of Anaplasma marginale and rickettsial endosymbionts in blood-sucking flies (Diptera: Tabanidae, Muscidae) and hard ticks (Acari: Ixodidae)
    Sándor Hornok
    Department of Parasitology and Zoology, Faculty of Veterinary Science, Szent Istvan University, Istvan u 2, 1078 Budapest, Hungary
    Vet Parasitol 154:354-9. 2008
    ..specific msp4 PCR, four individuals of T. bovinus collected near to grazing cattle were positive for Anaplasma marginale. The results of the present study provide the first molecular evidence for the potential mechanical vector ..
  18. doi Experimental transmission of field Anaplasma marginale and the A. centrale vaccine strain by Hyalomma excavatum, Rhipicephalus sanguineus and Rhipicephalus (Boophilus) annulatus ticks
    Varda Shkap
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan 50250, Israel
    Vet Microbiol 134:254-60. 2009
    The cattle rickettsia Anaplasma marginale is distributed worldwide and is transmitted by about 20 tick species, but only Rhipicephalus simus, a strictly African tick species, has been shown to transmit the vaccine strain of A. centrale...
  19. doi High-throughput identification of T-lymphocyte antigens from Anaplasma marginale expressed using in vitro transcription and translation
    Job E Lopez
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, United States
    J Immunol Methods 332:129-41. 2008
    ..An outer membrane fraction of the rickettsial pathogen Anaplasma marginale induces protective immunity against infection and disease in cattle...
  20. doi Induced immune response of DNA vaccine encoding an association MSP1a, MSP1b, and MSP5 antigens of Anaplasma marginale
    Flora S Kano
    Protozoology Laboratory, Departamento de Medicina Veterinaria Preventiva, CCA, Universidade de Londrina UEL, Londrina, Parana state, Brazil
    Vaccine 26:3522-7. 2008
    The outer membrane proteins of Anaplasma marginale have been the focus of research to obtain an improved vaccine against bovine anaplasmosis...
  21. ncbi Genetic diversity of Anaplasma marginale strains from cattle farms in the province of Palermo, Sicily
    J de la Fuente
    Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
    J Vet Med B Infect Dis Vet Public Health 52:226-9. 2005
    Bovine anaplasmosis, caused by the tick-borne rickettsia Anaplasma marginale, is endemic in Sicily and results in economic loss to the cattle industry. This study was designed to characterize strains of A...
  22. pmc Expression patterns of Anaplasma marginale Msp2 variants change in response to growth in cattle, and tick cells versus mammalian cells
    Adela S Oliva Chávez
    Department of Entomology, University of Minnesota, Saint Paul, Minnesota, USA
    PLoS ONE 7:e36012. 2012
    ..Likewise, major surface protein 2 (Msp2) of the tick-borne pathogen, Anaplasma marginale, is thought to be involved in antigenic variation to evade the mammalian host immune response...
  23. ncbi IgG and IgG2 antibodies from cattle naturally infected with Anaplasma marginale recognize the recombinant vaccine candidate antigens VirB9, VirB10, and elongation factor-Tu
    Flábio R Araújo
    Embrapa Gado de Corte, Campo Grande, MS, Brasil
    Mem Inst Oswaldo Cruz 103:186-90. 2008
    b>Anaplasma marginale is an important vector-borne rickettsia of ruminants in tropical and subtropical regions of the world. Immunization with purified outer membranes of this organism induces protection against acute anaplasmosis...
  24. pmc Rapid deletion of antigen-specific CD4+ T cells following infection represents a strategy of immune evasion and persistence for Anaplasma marginale
    Sushan Han
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
    J Immunol 181:7759-69. 2008
    ..We investigated this by using infection with Anaplasma marginale, a ruminant pathogen that replicates to levels of 10(9) bacteria per ml of blood during acute infection and ..
  25. ncbi Lack of infectivity of a Brazilian Anaplasma marginale isolate for Boophilus microplus ticks
    Patrícia M Gonçalves Ruiz
    Departamento de Medicina Veterinaria Preventiva, Escola de Veterinaria, Universidade Federal de Minas Gerais UFMG, C P 567, Belo Horizonte, 30123 970 Minas Gerais, Brazil
    Vet Parasitol 128:325-31. 2005
    Previous studies have shown that one Brazilian Anaplasma marginale isolate presents an inclusion appendage (tail), while other isolates do not present such inclusion...
  26. pmc Comparative genomics and transcriptomics of trait-gene association
    Sebastian Aguilar Pierle
    Program in Genomics, Department of Veterinary Microbiology and Pathology, Paul G, Allen School for Global Animal Health, Washington State University, Pullman, WA 99164 7040, USA
    BMC Genomics 13:669. 2012
    ..important tick-borne pathogens, from Rickettsia rickettsii, which causes Rocky Mountain spotted fever, to Anaplasma marginale, the most prevalent vector-borne pathogen of cattle...
  27. doi Cloning, expression, and characterization of the MSP1a and MSP1b recombinant proteins from PR1 Anaplasma marginale strain, Brazil
    Katia Tamekuni
    Universidade Estadual de Londrina, Departamento de Medicina Veterinaria Preventiva, Centro de Ciencias Agrarias, Pr 445 Km 380, Cx Postal 6001, 86051 990 Londrina, PR, Brazil
    Res Vet Sci 86:98-107. 2009
    The Anaplasma marginale is a bacterium that has obligate intraerythrocytic multiplication in cattle causing important economic loss. The A...
  28. pmc Conservation in the face of diversity: multistrain analysis of an intracellular bacterium
    Michael J Dark
    Program in Genomics, Department of Veterinary Microbiology and Pathology, School for Global Animal Health, Washington State University, Pullman, WA 99164 7040, USA
    BMC Genomics 10:16. 2009
    ..We determined the complete genome sequence of the Florida strain of Anaplasma marginale, and near complete (>96%) sequences for an additional three strains, for comparative analysis with the ..
  29. pmc The immunization-induced antibody response to the Anaplasma marginale major surface protein 2 and its association with protective immunity
    Susan M Noh
    Animal Disease Research Unit, Agricultural Research Service, U S Department of Agriculture, Pullman, WA 99164 7030, USA
    Vaccine 28:3741-7. 2010
    ..This is exemplified by Anaplasma marginale, a tick-borne bacterial pathogen that generates major surface protein 2 (Msp2) variants to provide for ..
  30. ncbi Structural basis for segmental gene conversion in generation of Anaplasma marginale outer membrane protein variants
    James E Futse
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Mol Microbiol 57:212-21. 2005
    ..In addition, Anaplasma marginale generates variants by recombination of oligonucleotide segments derived from the pseudogenes into the ..
  31. ncbi Genetic diversity and molecular phylogeny of Anaplasma marginale isolates from Minas Gerais, Brazil
    Jose De La Fuente
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA
    Vet Parasitol 121:307-16. 2004
    b>Anaplasma marginale (Rickettsiales: Anaplasmataceae), a tick-borne pathogen of cattle, is endemic in tropical and subtropical regions of the world, and many isolates of A. marginale may occur in a given geographic area...
  32. ncbi Immune response of BALB/c mouse immunized with recombinant MSPs proteins of Anaplasma marginale binding to immunostimulant complex (ISCOM)
    Paula M Kawasaki
    Universidade Estadual de Londrina, Departamento Medicina Veterinária Preventiva, CCA, Campus Universitario, Caixa Postal 6001, 86051 970 Londrina, PR, Brazil
    Res Vet Sci 83:347-54. 2007
    Anaplasmosis, caused by Anaplasma marginale, results in significant economic losses of cattle in tropical and subtropical regions worldwide...
  33. pmc Breadth of the CD4+ T cell response to Anaplasma marginale VirB9-1, VirB9-2 and VirB10 and MHC class II DR and DQ restriction elements
    Kaitlyn Morse
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Immunogenetics 64:507-23. 2012
    ..CD4+ T cell responses are important for controlling infection by many bacterial pathogens including Anaplasma marginale and are observed in cattle immunized with the protective A. marginale outer membrane (OM) vaccine...
  34. pmc Temporal characterisation of the organ-specific Rhipicephalus microplus transcriptional response to Anaplasma marginale infection
    Ricardo F Mercado-Curiel
    Department of Veterinary Microbiology and Pathology and Paul G Allen School for Global Animal Health, Washington State University, Pullman, WA 99164 7040, USA
    Int J Parasitol 41:851-60. 2011
    ..Using Anaplasma marginale infection of Rhipicephalus microplus as the experimental model, we tested three hypotheses exploring the ..
  35. doi Acute phase response in cattle infected with Anaplasma marginale
    S Nazifi
    Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
    Vet Microbiol 155:267-71. 2012
    ..and TNF-α) and acute phase proteins (Hp and SAA) in 20 adult crossbred cattle naturally infected by Anaplasma marginale. The infected animals were divided into 2 subgroups on the basis of parasitemia rate (<20% and >20%)...
  36. pmc Association and evidence for linked recognition of type IV secretion system proteins VirB9-1, VirB9-2, and VirB10 in Anaplasma marginale
    Kaitlyn Morse
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Paul G Allen School for Global Animal Health, Washington State University, Pullman, Washington, USA
    Infect Immun 80:215-27. 2012
    Like several other bacterial pathogens, Anaplasma marginale has an outer membrane that induces complete protection from infection and disease...
  37. ncbi Comparison of the efficiency of biological transmission of Anaplasma marginale (Rickettsiales: Anaplasmataceae) by Dermacentor andersoni Stiles (Acari: Ixodidae) with mechanical transmission by the horse fly, Tabanus fuscicostatus Hine (Diptera: Muscidae)
    Glen A Scoles
    USDA ARS, Animal Disease Research Unit, Washington State University, Pullman, WA 99164, USA
    J Med Entomol 45:109-14. 2008
    ..We compared the relative efficiencies of mechanical transmission of Anaplasma marginale by the horse fly, Tabanus fuscicostatus Hine, during acute infection (approximately 10(7) to approximately ..
  38. doi Clinical features associated with seroconversion to Anaplasma marginale, Babesia bigemina and Theileria parva infections in African cattle under natural tick challenge
    J W Magona
    National Livestock Resources Research Institute NaLIRRI, P O Box 96, Tororo, Uganda
    Vet Parasitol 155:273-80. 2008
    ..natural tick challenge, with a view to identifying clinical features for prediction of seroconversion to Anaplasma marginale, Babesia bigemina and Theileria parva infections...
  39. ncbi Diagnostic accuracy of methods for detecting Anaplasma marginale infection in lactating dairy cattle of Puerto Rico
    Geoffrey T Fosgate
    Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa
    J Vet Diagn Invest 22:192-9. 2010
    ..The objective of this study was to estimate the accuracy of assays for detection of Anaplasma marginale infection in lactating dairy cattle of Puerto Rico using Bayesian methods without a perfect reference test...
  40. ncbi Adaptations of the tick-borne pathogen, Anaplasma marginale, for survival in cattle and ticks
    Katherine M Kocan
    Department of Veterinary Pathobiology, 250 McElroy Hall, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078 2007, USA
    Exp Appl Acarol 28:9-25. 2002
    The tick-borne cattle pathogen Anaplasma marginale (Rickettsiales: Anaplasmataceae) multiplies within membrane-bound inclusions in host cell cytoplasm. Many geographic isolates of A...
  41. doi Genetic diversity of Anaplasma marginale in Argentina
    Paula Ruybal
    Instituto de Biotecnologia, Centro de Investigaciones de Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnologia Agropecuaria INTA, Los Reseros y Las Cabanas, CP 1686, Hurlingam, Buenos Aires, Argentina
    Vet Parasitol 162:176-80. 2009
    Bovine anaplasmosis caused by Anaplasma marginale is a worldwide major constraint to cattle production. The A...
  42. ncbi In vivo endothelial cell infection by Anaplasma marginale
    A D Carreño
    Department of Pathobiology, Auburn University, AL 36849 5519, USA
    Vet Pathol 44:116-8. 2007
    b>Anaplasma marginale has recently been shown to infect endothelial cells in vitro, but it remains unknown as to whether endothelial infection also occurs in vivo...
  43. doi Prevalence of Anaplasma marginale in different tick species from Ngorongoro Crater, Tanzania
    Robert D Fyumagwa
    Tanzania Wildlife Research Institute TAWIRI Wildlife Veterinary Programme, P O Box 661, Arusha, Tanzania
    Vet Parasitol 161:154-7. 2009
    ..acids extraction and a molecular analysis was performed to determine the range of tick species harboring Anaplasma marginale. The real-time PCR was used in the amplification of rickettsia DNA in tick pools (n=527) from 11 identified ..
  44. ncbi [Cloning, expression, molecular characterization of the MSP5 protein from PR1 strain of Anaplasma marginale and its application in a competitive enzyme-linked immunosorbent test]
    Elizabete Regina M Marana
    Departamento de Medicina Veterinaria Preventiva, Centro de Ciencias Agrarias, Universidade Estadual de Londrina UEL, Campus Universitario, Rod Celso Garcia Cid, PR 445, Km 380, CP 6001, CEP 86051 990 Londrina, PR, Brasil
    Rev Bras Parasitol Vet 18:5-12. 2009
    ..enzyme-linked immunosorbent test using the PR1 recombinant major surface protein 5 (rMSP5-PR1-ELISA) of Anaplasma marginale was standardized and validated using sera from anaplasmosis free and endemic regions...
  45. doi Concomitant infection of cattle with the vaccine strain Anaplasma marginale ss centrale and field strains of A. marginale
    Varda Shkap
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
    Vet Microbiol 130:277-84. 2008
    Bovine anaplasmosis, caused by Anaplasma marginale, the intraerythrocytic rickettsia, is controlled by vaccination with live Anaplasma marginale ss centrale (A. centrale), a subspecies of relatively low pathogenicity...
  46. doi Anaplasma marginale Yucatan (Mexico) Strain. Assessment of low virulence and potential use as a live vaccine
    Sergio D Rodríguez Camarillo
    Centro de Investigaciones Disciplinarias, en Parasitología Veterinaria, Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, Secretaría de Agricultura, Ganaderia, Desarrollo Rural, Pesca y Alimentació, Jiutepec, Morelos, Mexcio
    Ann N Y Acad Sci 1149:98-102. 2008
    b>Anaplasma marginale Yucatan strain was found to have low virulence in cattle...
  47. pmc Complete genome sequence of Anaplasma marginale subsp. centrale
    David R Herndon
    Animal Diseases Research Unit, Agricultural Research Service, U S Department of Agriculture, Pullman, Washington 99164, USA
    J Bacteriol 192:379-80. 2010
    b>Anaplasma marginale subsp. centrale is a naturally attenuated subtype that has been used as a vaccine for a century. We sequenced the genome of this organism and compared it to those of virulent senso stricto A. marginale strains...
  48. ncbi Analysis of membrane protein genes in a Brazilian isolate of Anaplasma marginale
    Daniel S G Junior
    Universidade Federal Rural do Rio de Janeiro, Seropedica, RJ, Brasil
    Mem Inst Oswaldo Cruz 105:843-9. 2010
    The sequencing of the complete genome of Anaplasma marginale has enabled the identification of several genes that encode membrane proteins, thereby increasing the chances of identifying candidate immunogens...
  49. pmc Differential expression of genes in salivary glands of male Rhipicephalus (Boophilus)microplus in response to infection with Anaplasma marginale
    Zorica Zivkovic
    Department of Infectious Diseases and Immunology, Utrecht Centre for Tick borne Diseases UCTD, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584CL Utrecht, The Netherlands
    BMC Genomics 11:186. 2010
    Bovine anaplasmosis, caused by the rickettsial tick-borne pathogen Anaplasma marginale (Rickettsiales: Anaplasmataceae), is vectored by Rhipicephalus (Boophilus)microplus in many tropical and subtropical regions of the world. A...
  50. pmc Identification of Anaplasma marginale outer membrane protein antigens conserved between A. marginale sensu stricto strains and the live A. marginale subsp. centrale vaccine
    Joseph T Agnes
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 79:1311-8. 2011
    Live vaccination with Anaplasma marginale subsp. centrale (synonym for Anaplasma centrale) induces protection against severe disease upon challenge with A. marginale sensu stricto strains...
  51. doi Genetic diversity of major surface protein 1a of Anaplasma marginale in beef cattle
    T Molad
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan 50250, Israel
    Vet Microbiol 136:54-60. 2009
    ..Learning how this diversity is generated and identification of distinct A. marginale strains coupled with high sequence variation of MSP1a will aid in understanding Anaplasma transmission and disease development...
  52. pmc Composition of the surface proteome of Anaplasma marginale and its role in protective immunity induced by outer membrane immunization
    Susan M Noh
    Animal Disease Research Unit, Agriculture Research Service, U S Department of Agriculture, Pullman, WA 99164 7030, USA
    Infect Immun 76:2219-26. 2008
    ..In this study, the surface proteome of Anaplasma marginale, a tick-transmitted bacterial pathogen, was targeted by using surface-specific cross-linking to form ..
  53. pmc Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins
    Kelly A Brayton
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washingston State University, Pullman, WA 99164 7040, USA
    Proc Natl Acad Sci U S A 102:844-9. 2005
    The rickettsia Anaplasma marginale is the most prevalent tick-borne livestock pathogen worldwide and is a severe constraint to animal health. A...
  54. pmc Superinfection as a driver of genomic diversification in antigenically variant pathogens
    James E Futse
    Program in Vector Borne Diseases, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA
    Proc Natl Acad Sci U S A 105:2123-7. 2008
    ....
  55. ncbi Potential vertebrate reservoir hosts and invertebrate vectors of Anaplasma marginale and A. phagocytophilum in central Spain
    Jose De La Fuente
    Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ciudad Real, Spain
    Vector Borne Zoonotic Dis 5:390-401. 2005
    ..The increasing contact between wildlife, domestic animals, and human populations increases the risk of outbreaks of human and bovine anaplasmosis, and the difficulty of implementing surveillance and control measures...
  56. pmc Anaplasma marginale infection with persistent high-load bacteremia induces a dysfunctional memory CD4+ T lymphocyte response but sustained high IgG titers
    Sushan Han
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Clin Vaccine Immunol 17:1881-90. 2010
    ..b>Anaplasma marginale is a persistent infection of cattle characterized by acute and chronic high-load bacteremia...
  57. pmc Identification of Anaplasma marginale proteins specifically upregulated during colonization of the tick vector
    Solomon S Ramabu
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology and School for Global Animal Health, Washington State University, Pullman, Washington 99164 7040, USA
    Infect Immun 78:3047-52. 2010
    ..We used an unbiased proteomic screen to identify Anaplasma marginale proteins specifically upregulated in the tick compared to the mammalian host...
  58. ncbi Molecular and serological detection of A. centrale- and A. marginale-infected cattle grazing within an endemic area
    T Molad
    Division of Parasitology, Kimron Veterinary Institute, P O Box 12, Bet Dagan 50250, Israel
    Vet Microbiol 113:55-62. 2006
    ..line blot hybridization (RLB) one-stage nested PCR (nPCR) for Anaplasma centrale and a nested PCR for Anaplasma marginale were used to detect infected cattle grazing within an endemic region in Israel...
  59. pmc Immunogenicity of Anaplasma marginale type IV secretion system proteins in a protective outer membrane vaccine
    Job E Lopez
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 75:2333-42. 2007
    ..Immunization with purified outer membranes induces protection against acute Anaplasma marginale infection and disease, and a proteomic and genomic approach recently identified 21 proteins within the ..
  60. pmc Anaplasma marginale type IV secretion system proteins VirB2, VirB7, VirB11, and VirD4 are immunogenic components of a protective bacterial membrane vaccine
    Eric L Sutten
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 78:1314-25. 2010
    ..Immunization of cattle with an Anaplasma marginale fraction enriched in outer membranes (OM) can provide complete protection against disease and persistent ..
  61. pmc Identification of novel antigenic proteins in a complex Anaplasma marginale outer membrane immunogen by mass spectrometry and genomic mapping
    Job E Lopez
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 73:8109-18. 2005
    Immunization with purified Anaplasma marginale outer membranes induces complete protection against infection that is associated with CD4+ T-lymphocyte-mediated gamma interferon secretion and immunoglobulin G2 (IgG2) antibody titers...
  62. doi Validation of an Anaplasma marginale cELISA for use in the diagnosis of A. ovis infections in domestic sheep and Anaplasma spp. in wild ungulates
    Glen A Scoles
    Animal Disease Research Unit, USDA ARS, Pullman, WA 99164, USA
    Vet Microbiol 130:184-90. 2008
    A commercially available (cELISA) kit for diagnosing Anaplasma marginale infection in cattle was validated for diagnosing A ovis infection in sheep using the bovine serum controls as supplied by the manufacturer (BcELISA) and sheep serum ..
  63. ncbi Comparison of three oxytetracycline regimes for the treatment of persistent Anaplasma marginale infections in beef cattle
    Johann F Coetzee
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Vet Parasitol 127:61-73. 2005
    Anaplasmosis, caused by the tick-borne rickettsia, Anaplasma marginale, is an economically important disease of cattle in the United States and worldwide...
  64. ncbi Anaplasma infection in free-ranging Iberian red deer in the region of Castilla-La Mancha, Spain
    Jose De La Fuente
    Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13005 Ciudad Real, Spain
    Vet Microbiol 100:163-73. 2004
    ..The type species, Anaplasma marginale, causes bovine anaplasmosis and infects erythrocytes of the vertebrate host and undergoes a complex ..
  65. ncbi Detection and quantification of Anaplasma marginale DNA in blood samples of cattle by real-time PCR
    G Carelli
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 124:107-14. 2007
    A TaqMan-based real-time PCR assay was developed for the diagnosis of Anaplasma marginale infection of cattle...
  66. ncbi Amplification of 16S rRNA genes of Anaplasma species in China for phylogenetic analysis
    Zhijie Liu
    Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 11, Lanzhou, Gansu 730 046, China
    Vet Microbiol 107:145-8. 2005
    ..was inferred through comparing five 16S rRNA gene sequences of four isolates of Anaplasma ovis and one of Anaplasma marginale in China with all nineteen 16S rRNA gene sequences deposited in GenBank (12 A. marginale, 3 A...
  67. ncbi Detection of antibodies against Anaplasma marginale in milk using a recombinant MSP5 indirect ELISA
    Susana Torioni de Echaide
    Instituto Nacional de Tecnologia Agropecuaria, Estacion Experimental Agropecuaria Rafaela, Ruta 34 km 227, CP 2300 Rafaela, Santa Fe, Argentina
    Vet Microbiol 106:287-92. 2005
    ..The recombinant 19 kDa major surface protein 5 (rMSP5) of Anaplasma marginale was used as antigen...
  68. ncbi Comparative gene expression by WC1+ gammadelta and CD4+ alphabeta T lymphocytes, which respond to Anaplasma marginale, demonstrates higher expression of chemokines and other myeloid cell-associated genes by WC1+ gammadelta T cells
    Kevin K Lahmers
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    J Leukoc Biol 80:939-52. 2006
    ..which respond to major surface protein 2 (MSP2) of the intraerythrocytic rickettsial pathogen of cattle, Anaplasma marginale. Gene expression profiles of activated T cell clones were compared using a microarray format, and ..
  69. ncbi Anaplasma marginale (Rickettsiales: Anaplasmataceae): recent advances in defining host-pathogen adaptations of a tick-borne rickettsia
    K M Kocan
    Department of Veterinary Pathobiology, 250 McElroy Hall, Oklahoma State University, Stillwater, OK 74078 2007, USA
    Parasitology 129:S285-300. 2004
    The tick-borne intracellular pathogen Anaplasma marginale (Rickettsiales: Anaplasmataceae) develops persistent infections in cattle and tick hosts. While erythrocytes appear to be the only site of infection in cattle, A...
  70. ncbi Insights into mechanisms of bacterial antigenic variation derived from the complete genome sequence of Anaplasma marginale
    Guy H Palmer
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Ann N Y Acad Sci 1078:15-25. 2006
    ..The resulting combinatorial diversity generates the number of variants both predicted and shown to emerge during persistence...
  71. ncbi First serological and molecular evidence on the endemicity of Anaplasma ovis and A. marginale in Hungary
    Sándor Hornok
    Department of Parasitology and Zoology, Faculty of Veterinary Science, Szent Istvan University, Istvan u 2, Budapest, Hungary
    Vet Microbiol 122:316-22. 2007
    ..from 9 farms in the region were examined serologically with a competitive ELISA to detect antibodies to Anaplasma marginale, A. centrale and A. ovis. The seropositivity in sheep was 99.4%, and in cattle 80.8%. A. ovis and A...
  72. ncbi Analysis of world strains of Anaplasma marginale using major surface protein 1a repeat sequences
    Jose De La Fuente
    Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078 2007, USA
    Vet Microbiol 119:382-90. 2007
    b>Anaplasma marginale is a tick-borne pathogen of cattle that causes the disease bovine anaplasmosis worldwide...
  73. ncbi Conservation of transmission phenotype of Anaplasma marginale (Rickettsiales: Anaplasmataceae) strains among Dermacentor and Rhipicephalus ticks (Acari: Ixodidae)
    Glen A Scoles
    USDA ARS, Animal Disease Research Unit, Washington State University, Pullman, WA 99164, USA
    J Med Entomol 44:484-91. 2007
    ..and Rhipicephalus (Boophilus) annulatus (Say) were important biological vectors of the cattle pathogen Anaplasma marginale Theiler. In the absence of Boophilus ticks, A. marginale continues to be transmitted by Dermacentor ticks...
  74. ncbi Capillary tube feeding system for studying tick-pathogen interactions of Dermacentor variabilis (Acari: Ixodidae) and Anaplasma marginale (Rickettsiales: Anaplasmataceae)
    Katherine M Kocan
    Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078 2007, USA
    J Med Entomol 42:864-74. 2005
    ..for studying the interaction between Dermacentor variabilis (Say) and the rickettsial cattle pathogen Anaplasma marginale Theiler. A...
  75. ncbi Genetic diversity of anaplasma species major surface proteins and implications for anaplasmosis serodiagnosis and vaccine development
    Jose De La Fuente
    Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078 2007, USA
    Anim Health Res Rev 6:75-89. 2005
    ..Information concerning the genetic diversity of Anaplasma spp. MSPs will likely influence the development of serodiagnostic assays and vaccine strategies for the control of anaplasmosis...
  76. ncbi Distinctly different msp2 pseudogene repertoires in Anaplasma marginale strains that are capable of superinfection
    Jose Luis Rodriguez
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Gene 361:127-32. 2005
    Lifelong persistent infection of cattle is a hallmark of the tick transmitted pathogen Anaplasma marginale. Antigenic variation of Major Surface Protein 2 (MSP2) plays an important role in evasion of the host immune response to allow ..
  77. pmc Selection for simple major surface protein 2 variants during Anaplasma marginale transmission to immunologically naïve animals
    Guy H Palmer
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 75:1502-6. 2007
    b>Anaplasma marginale, a rickettsial pathogen, evades clearance in the animal host by antigenic variation. Under immune selection, A. marginale expresses complex major surface protein 2 mosaics, derived from multiple donor sequences...
  78. ncbi Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale
    Jeffrey R Abbott
    Program in Vector borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, 99164, USA
    J Immunol 174:6702-15. 2005
    ..This study tested the hypothesis that challenge of cattle with Anaplasma marginale expressing MSP2 variants to which the animals had been immunized, would stimulate variant epitope-specific ..
  79. pmc Maintenance of antibody to pathogen epitopes generated by segmental gene conversion is highly dynamic during long-term persistent infection
    Yan Zhuang
    Program in Vector Borne Diseases and Immunology, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 7040, USA
    Infect Immun 75:5185-90. 2007
    ..This question was addressed by tracking the Anaplasma marginale variant population and corresponding segment-specific immunoglobulin G (IgG) antibody responses during long-..
  80. pmc Generation of antigenic variants via gene conversion: Evidence for recombination fitness selection at the locus level in Anaplasma marginale
    James E Futse
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, 99164, USA
    Infect Immun 77:3181-7. 2009
    ..We examined allelic usage in generating Anaplasma marginale variants during in vivo infection in the mammalian reservoir host and identified preferential usage of ..
  81. ncbi Differential expression of the msp1alpha gene of Anaplasma marginale occurs in bovine erythrocytes and tick cells
    Jose C Garcia-Garcia
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA
    Vet Microbiol 98:261-72. 2004
    Major surface proteins (MSP) 1a and 1b of the tick-borne pathogen Anaplasma marginale (Rickettsiales: Anaplasmataceae) are conserved on A. marginale derived from bovine erythrocytes and tick cells...
  82. ncbi Mapping of B-cell epitopes in the N-terminal repeated peptides of Anaplasma marginale major surface protein 1a and characterization of the humoral immune response of cattle immunized with recombinant and whole organism antigens
    Jose C Garcia-Garcia
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078 2007, USA
    Vet Immunol Immunopathol 98:137-51. 2004
    Major surface protein (MSP) 1a of the genus type species Anaplasma marginale (Rickettsiales: Anaplasmataceae) together with MSP1b forms the MSP1 complex...
  83. doi Silencing expression of the defensin, varisin, in male Dermacentor variabilis by RNA interference results in reduced Anaplasma marginale infections
    Katherine M Kocan
    Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, USA
    Exp Appl Acarol 46:17-28. 2008
    ..research, we studied the role of varisin in the immunity of ticks to the gram-negative cattle pathogen, Anaplasma marginale. Expression of the varisin gene was silenced by RNA interference (RNAi) in which male ticks were injected ..
  84. pmc Antigens and alternatives for control of Anaplasma marginale infection in cattle
    Katherine M Kocan
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    Clin Microbiol Rev 16:698-712. 2003
    Anaplasmosis, a tick-borne cattle disease caused by the rickettsia Anaplasma marginale, is endemic in tropical and subtropical areas of the world...
  85. doi Differential expression of the tick protective antigen subolesin in anaplasma marginale- and A. phagocytophilum-infected host cells
    Jose De La Fuente
    Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma, USA
    Ann N Y Acad Sci 1149:27-35. 2008
    ..and to affect tick feeding, reproduction, and development as well as infection of host cells by Anaplasma marginale and A. phagocytophilum...
  86. doi Propagation of a Brazilian isolate of Anaplasma marginale with appendage in a tick cell line (BME26) derived from Rhipicephalus (Boophilus) microplus
    E Esteves
    Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Avenida Prof Lineu Prestes 1374, CEP 05508 900, Sao Paulo, Brazil
    Vet Parasitol 161:150-3. 2009
    b>Anaplasma marginale is a tick-borne pathogen of cattle responsible for the disease anaplasmosis. Data suggest that Rhipicephalus (Boophilus) microplus and R. annulatus may be the major tick vectors of A...
  87. pmc Independence of Anaplasma marginale strains with high and low transmission efficiencies in the tick vector following simultaneous acquisition by feeding on a superinfected mammalian reservoir host
    Maria F B M Galletti
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 USA
    Infect Immun 77:1459-64. 2009
    Strain superinfection occurs when a second pathogen strain infects a host already carrying a primary strain. Anaplasma marginale superinfection occurs when the second strain carries a variant repertoire different from that of the primary ..
  88. ncbi Infection of endothelial cells with Anaplasma marginale and A. phagocytophilum
    Ulrike G Munderloh
    University of Minnesota, Department of Entomology, 219 Hodson Hall, 1980 Folwell Ave, St Paul, MN 55108, USA
    Vet Microbiol 101:53-64. 2004
    b>Anaplasma marginale and A. phagocytophilum are obligate intracellular, tick-borne pathogens that target erythrocytes and neutrophil granulocytes, respectively...
  89. pmc Concurrent infections with vector-borne pathogens associated with fatal hemolytic anemia in a cattle herd in Switzerland
    Regina Hofmann-Lehmann
    Clinical Laboratory, University of Zurich, Switzerland
    J Clin Microbiol 42:3775-80. 2004
    ..Upon microscopic examination of red blood cells, Anaplasma marginale inclusion bodies were found in 47% of the cows...
  90. ncbi The CD4+ T cell immunodominant Anaplasma marginale major surface protein 2 stimulates gammadelta T cell clones that express unique T cell receptors
    Kevin K Lahmers
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    J Leukoc Biol 77:199-208. 2005
    Major surface protein 2 (MSP2) of the bovine rickettsial pathogen Anaplasma marginale is an abundant, serologically immunodominant outer membrane protein...
  91. pmc Stochastic transmission of multiple genotypically distinct Anaplasma marginale strains in a herd with high prevalence of Anaplasma infection
    Guy H Palmer
    Program in Vector Borne Diseases, Washington State University, Pullman, WA 99164 7040, USA
    J Clin Microbiol 42:5381-4. 2004
    Multiple genotypically unique strains of the tick-borne pathogen Anaplasma marginale occur and are transmitted within regions where the organism is endemic. In this study, we tested the hypothesis that specific A...
  92. pmc Identification of a novel Anaplasma marginale appendage-associated protein that localizes with actin filaments during intraerythrocytic infection
    Roger W Stich
    Department of Veterinary Preventive Medicine, The Ohio State University, 1900 Coffey Rd, Columbus, OH 43210, USA
    Infect Immun 72:7257-64. 2004
    The rickettsial pathogen Anaplasma marginale assembles an actin filament bundle during intracellular infection. Unlike other bacterial pathogens that generate actin filament tails, A...
  93. pmc Anaplasma marginale major surface protein 2 CD4+-T-cell epitopes are evenly distributed in conserved and hypervariable regions (HVR), whereas linear B-cell epitopes are predominantly located in the HVR
    Jeffrey R Abbott
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
    Infect Immun 72:7360-6. 2004
    ..Throughout Anaplasma marginale infection, recombination results in the sequential appearance of novel MSP2 variants and subsequent control ..
  94. pmc Glycosylation of Anaplasma marginale major surface protein 1a and its putative role in adhesion to tick cells
    Jose C Garcia-Garcia
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078 2007, USA
    Infect Immun 72:3022-30. 2004
    b>Anaplasma marginale, the causative agent of bovine anaplasmosis, is a tick-borne rickettsial pathogen of cattle that multiplies in erythrocytes and tick cells. Major surface protein 1a (MSP1a) and MSP1b form the MSP1 complex of A...
  95. ncbi Seroprevalence of anaplasmosis among cattle in Switzerland in 1998 and 2003: no evidence of an emerging disease
    Ute M Dreher
    Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, CH 8057 Zurich, Switzerland
    Vet Microbiol 107:71-9. 2005
    b>Anaplasma marginale infection in Europe has been limited to the Mediterranean and eastern countries, to Austria and to very sporadic cases in Switzerland. There are no reports of its occurrence in the countries north of Switzerland...
  96. ncbi Functional genomic studies of tick cells in response to infection with the cattle pathogen, Anaplasma marginale
    Jose De La Fuente
    Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
    Genomics 90:712-22. 2007
    ..these studies, we used a functional genomics approach to characterize tick genes regulated in response to Anaplasma marginale infection...
  97. doi Evaluation of sequential coinfection with Anaplasma phagocytophilum and Anaplasma marginale in cattle
    Bruce R Hoar
    Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
    Am J Vet Res 69:1171-8. 2008
    To determine whether sequelae of infection differed among single versus double infection with Anaplasma phagocytophilum or Anaplasma marginale, with and without tick salivary extract, in cattle.
  98. pmc Tick-borne transmission of two genetically distinct Anaplasma marginale strains following superinfection of the mammalian reservoir host
    Christina K Leverich
    Department of Veterinary Microbiology and Pathology, Program in Vector Borne Diseases, School for Global Animal Health, Washington State University, Pullman, Washington 99164 7040, USA
    Infect Immun 76:4066-70. 2008
    ..has recently been shown to occur for two genetically distinct strains of the tick-borne pathogen Anaplasma marginale that encode distinctly different surface protein variants...
  99. pmc Phylogeographic analysis reveals association of tick-borne pathogen, Anaplasma marginale, MSP1a sequences with ecological traits affecting tick vector performance
    Agustin Estrada-Pena
    Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain
    BMC Biol 7:57. 2009
    The tick-borne pathogen Anaplasma marginale, which is endemic worldwide, is the type species of the genus Anaplasma (Rickettsiales: Anaplasmataceae). Rhipicephalus (Boophilus) microplus is the most important tick vector of A...
  100. ncbi Comparison of the complement fixation test and competitive ELISA for serodiagnosis of Anaplasma marginale infection in experimentally infected steers
    Johann F Coetzee
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Am J Vet Res 68:872-8. 2007
    To compare sensitivity of a complement fixation (CF) test and competitive ELISA (cELISA) for detection of Anaplasma marginale in experimentally infected steers.
  101. ncbi Development of an immunosensor for the diagnosis of bovine anaplasmosis
    Marta Silva
    Instituto de Biologia Experimental e Tecnológica Instituto de Tecnologia Química e Biológica, Apartado 12, P 2781 901 Oeiras, Portugal
    Ann N Y Acad Sci 1081:379-81. 2006
    An optical immunosensor based in major surface protein 5 (MSP5) of Anaplasma marginale was developed towards detection of anti-Anaplasma sp. antibodies in acute infection as well as in vaccinated cattle...

Research Grants84

  1. Antigenic variation of Anaplasma marginale
    PATRICK MEEUS; Fiscal Year: 2003
    ..b>Anaplasma marginale, a bovine pathogen, is closely related to Anaplasma (Ehrlichia) phagocytophila, the causative agent of ..
  2. MOLECULAR MECHANISMS OF MSP2 VARIATION IN RICKETTSIAE
    Anthony Barbet; Fiscal Year: 2007
    ..MSP2 was initially defined in Anaplasma marginale and infections of cattle and ticks with this pathogen provide an excellent model for discovering the ..
  3. MOLECULAR MECHANISMS OF MSP2 VARIATION IN RICKETTSIAE
    Anthony Barbet; Fiscal Year: 2003
    ..MSP2 was initially defined in Anaplasma marginale and infections of cattle and ticks with this pathogen provide an excellent model for discovering the ..
  4. MOLECULAR MECHANISMS OF MSP2 VARIATION IN RICKETTSIAE
    Anthony Barbet; Fiscal Year: 2006
    ..MSP2 was initially defined in Anaplasma marginale and infections of cattle and ticks with this pathogen provide an excellent model for discovering the ..
  5. MOLECULAR MECHANISMS OF MSP2 VARIATION IN RICKETTSIAE
    Anthony Barbet; Fiscal Year: 2002
    ..canis, E. equi, Cowdria ruminantium and Anaplasma marginale infections of animals...
  6. Immunity directed against MSP 2 during anaplasmosis
    Jeffrey Abbott; Fiscal Year: 2004
    ..b>Anaplasma marginale is closely related genetically (16S rRNA) with E...
  7. Immunogenicity of the Type IV Secretin System
    WENDY CATHERINE BROWN; Fiscal Year: 2010
    ..We propose to use the Anaplasma marginale model to test the hypothesis that immunization with a linked immunogen composed of naturally associated ..
  8. Identification of T-Cell Immunogens in Anaplasma
    Wendy Brown; Fiscal Year: 2007
    ..To date, only four T cell immunostimulatory molecules have been identified in Anaplasma marginale, the type species, and none in the related human pathogens within the Anaplasmataceae...
  9. Immunogenicity of the Type IV Secretin System
    Wendy Brown; Fiscal Year: 2009
    ..We propose to use the Anaplasma marginale model to test the hypothesis that immunization with a linked immunogen composed of naturally associated ..
  10. IMMUNOLOGY TRAINING PROGRAM
    Guy Palmer; Fiscal Year: 2007
    ..The program is based in the College of Veterinary Medicine and is enhanced by interaction with faculty in the School of Molecular Biosciences. ..
  11. Antigenic variation in rickettsial transmission
    Guy Palmer; Fiscal Year: 2007
    ..This hypothesis will be addressed using comprehensive identification of the variant population in the reservoir host and tick vector and testing whether immune responses against a broad array of variants prevents superinfection. ..
  12. ANTIGENIC VARIATION IN EHRLICHIAL TRANSMISSION
    Guy Palmer; Fiscal Year: 2003
    ..The system under study is Anaplasma marginale, a member of the ehrlichial genogroup II, and its transmission by the ixodid tick Dermacentor andersoni...
  13. Genetic Determinants of Anaplasma Transmission
    SUSAN NOH; Fiscal Year: 2004
    ..I propose to study transmissible and non-transmissible strains of Anaplasma marginale as a model to identify the molecular determinants of Anaplasma spp. transmission...
  14. MOLECULAR BASIS OF PERSISTENT EHRLICHIA INFECTION
    XUE JIE YU; Fiscal Year: 2004
    ..chaffeensis. This will provide valuable information regarding the potential use of these antigens as subunit vaccines. ..
  15. Comparison of Human Ehrlichiosis Agent Genomes
    Yasuko Rikihisa; Fiscal Year: 2007
    ..chaffeensis virulence determinants and their pathogenic mechanisms. The results may point to potential chemotherapy, chemopreventive and/or vaccine candidates for treatment and prevention of human ehrlichiosis. ..
  16. ROLES OF EHRLICHIAL OUTER MEMBRANE PROTEIN IN PARASITISM
    Yasuko Rikihisa; Fiscal Year: 2001
    ..Aim 5 addresses hypothesis 3 by examining the effects of immunization with the ehrlichial OMP in a mouse model of human ehrlichiosis. ..
  17. MOLECULAR BASIS OF BORRELIA PATHOGENESIS
    Alan Barbour; Fiscal Year: 2007
    ..We will evaluate this by developing models that are based on currently available data about infection dynamics and then use these early models' deficiencies to direct us in further experimentation and modelbuilding. ..
  18. Development of Paratransgenic ticks for Disease Control
    Ulrike Munderloh; Fiscal Year: 2009
    ..Our research will identify ways in which these symbionts can be exploited to reduce transmission of tick-borne disease agents to humans. ..
  19. Comparison of Human Ehrlichiosis Agent Genomes
    Yasuko Rikihisa; Fiscal Year: 2010
    ..chaffeensis virulence determinants and their pathogenic mechanisms. The results may point to potential chemotherapy, chemopreventive and/or vaccine candidates for treatment and prevention of human ehrlichiosis. ..
  20. Infection Dynamics of Ehrlichia chaffeensis
    Andrea Varela Stokes; Fiscal Year: 2006
    ..Varela to develop into an outstanding medical parasitologist and add to the understanding of tick-borne disease and human health. ..
  21. The Role of Plasmids in Rickettsia Biology
    ULRIKE GERTRUD MUNDERLOH; Fiscal Year: 2010
    ..The results of this research may provide new tools to control Rickettsia infections of humans. ..
  22. Development of Paratransgenic ticks for Disease Control
    ULRIKE GERTRUD MUNDERLOH; Fiscal Year: 2010
    ..Our research will identify ways in which these symbionts can be exploited to reduce transmission of tick-borne disease agents to humans. ..
  23. Tick-Mammal Interface of Human Granulocytic Anaplasmosis
    ULRIKE GERTRUD MUNDERLOH; Fiscal Year: 2010
    ..Taken together, our research will continue to identify new leads on how to prevent and cure human anaplasmosis and similar diseases such as ehrlichiosis and spotted fevers. ..
  24. PATHOGENESIS OF HUMAN GRANULOCYTIC EHRLICHIOSIS
    Ulrike Munderloh; Fiscal Year: 2001
    ....
  25. Tick-Mammal Interface of Human Granulocytic Anaplasmosis
    Ulrike Munderloh; Fiscal Year: 2009
    ..Surface association of Ap and relationship to cellular markers will be studied by FESEM. To identify Ap adhesion proteins, we will screen a cDNA library with blocking antisera, but focus on p100, p130 and MSP2 variants. ..
  26. Anaplasma marginale genes determining tick transmission
    MICHAEL DARK; Fiscal Year: 2007
    ..The sponsors collaborate closely in research and have successfully mentored clinicians, graduate students, and post-doctoral fellows to research independence. ..
  27. Identifying human genes required by tick-borne pathogens
    Ulrike Munderloh; Fiscal Year: 2007
    ..We anticipate that the interaction of the human host cells with these highly specialized intracellular pathogens will shed light,on the nature of some unidentified human genes. ..
  28. Development of Paratransgenic ticks for Disease Control
    Ulrike Munderloh; Fiscal Year: 2006
    ..Finally, we will work towards 3. stable transformation of R. peacockii with cecropinA. We will then test the transfonnants for antimicrobial activity in vitro and in ticks, and characterize them by sequence analysis. ..