Genomes and Genes
Summary: A species of gram-positive, aerobic bacteria non-pathogenic for humans. It is the etiologic agent of Johne's disease (see PARATUBERCULOSIS).
Publications190 found, 100 shown here
- Age-specific characteristics of ELISA and fecal culture for purpose-specific testing for paratuberculosisS S Nielsen
Department of Large Animal Sciences, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark
J Dairy Sci 89:569-79. 2006..Purpose-specific tests are required to appropriately interpret and use test results for management of paratuberculosis, and relevant covariates, such as age, should be included when possible...
- Testing the interaction between NOD-2 status and serological response to Mycobacterium paratuberculosis in cases of inflammatory bowel diseaseCharles N Bernstein
Department of Internal Medicine, University of Manitoba Inflammatory Bowel Disease Clinical and Research Centre, Winnipeg, Manitoba, Canada
J Clin Microbiol 45:968-71. 2007..In the same population groups, we assessed the seroprevalence for Mycobacterium paratuberculosis and found it to be 35%, with no differences between CD, ulcerative colitis (UC), and controls...
- Early induction of humoral and cellular immune responses during experimental Mycobacterium avium subsp. paratuberculosis infection of calvesW R Waters
Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa 50010, USA
Infect Immun 71:5130-8. 2003..Additionally, this study confirms that mycobacteria-specific antibody is detectable early in the course of experimental Johne's disease, even preceding the development of specific cell-mediated responses...
- Evaluation of five antibody detection tests for diagnosis of bovine paratuberculosisMichael T Collins
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, 2015 Linden Dr, Madison, WI 53706 1102, USA
Clin Diagn Lab Immunol 12:685-92. 2005..samples from 359 dairy cattle in seven paratuberculosis-free herds and 2,094 dairy cattle in seven Mycobacterium paratuberculosis-infected dairy herds...
- Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samplesYing Fang
Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA
J Clin Microbiol 40:287-91. 2002..Results were quantitated by using a standard curve derived from a plasmid containing IS900. A minimum quantity of 1.7 x 10(-4) pg of DNA, correlating to 1 to 8 CFU, was detected...
- Persistence of Mycobacterium paratuberculosis during manufacture and ripening of cheddar cheeseJ A Donaghy
Agriculture, Food and Environmental Science Division Food Microbiology Branch, Department of Agriculture and Rural Development for N Ireland, Newforge Lane, Belfast BT9 5PX, N Ireland, United Kingdom
Appl Environ Microbiol 70:4899-905. 2004..with high 10(4) to 10(5) CFU/ml) and low (10(1) to 10(2) CFU/ml) inocula of three different Mycobacterium paratuberculosis strains...
- Real-time quantitative PCR detection of Mycobacterium avium subsp. paratuberculosis and differentiation from other mycobacteria using SYBR Green and TaqMan assaysSubbarao V Ravva
United States Department of Agriculture, Agriculture Research Service, Western Regional Research Center, Foodborne Contaminants Research Unit, 800 Buchanan St, Albany, CA 94710, USA
J Microbiol Methods 63:305-17. 2005..No reporter signal was detected during TaqMan assays performed with 100 pg of template DNA from the non-Map organisms...
- Development of an F57 sequence-based real-time PCR assay for detection of Mycobacterium avium subsp. paratuberculosis in milkT Tasara
Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Winterthurerstr 272, CH 8057 Zurich, Switzerland
Appl Environ Microbiol 71:5957-68. 2005..avium subsp. paratuberculosis-infected cows, as well as pooled samples from a dairy herd with a confirmed history of paratuberculosis...
- The use of spiral plating and microscopic colony counting for the rapid quantitation of Mycobacterium paratuberculosisW L Smith
Dairy Food Safety Laboratory, School of Veterinary Medicine, Veterinary Medicine and Teaching Research Center, University of California, Davis, CA 95616, USA
Lett Appl Microbiol 36:293-6. 2003To evaluate a spiral plating and microscopic colony counting technique to hasten the quantitation of Mycobacterium paratuberculosis.
- Cloning, expression, purification and serodiagnostic evaluation of fourteen Mycobacterium paratuberculosis proteinsDonghee Cho
School of Veterinary Medicine, University of Wisconsin Madison, 2015 Linden Drive, Madison, WI 53706, USA
Protein Expr Purif 53:411-20. 2007..of potential diagnostic value for bovine paratuberculosis were identified in the culture filtrate of Mycobacterium paratuberculosis JTC303 by immunoblot and mass spectrometry...
- Temporal Mycobacterium paratuberculosis infection in T-cell receptor (TCR)-alpha and TCR-delta-deficient miceJ R Stabel
National Disease of Livestock Research Unit, National Animal Disease Center, USDA ARS, Ames, IA 50010, USA
Vet Immunol Immunopathol 89:127-32. 2002b>Mycobacterium paratuberculosis is the causative agent of paratuberculosis (Johne's disease), a chronic inflammation of the terminal portion of the ileum in ruminants...
- Multilocus short sequence repeat sequencing approach for differentiating among Mycobacterium avium subsp. paratuberculosis strainsAlongkorn Amonsin
Department of Microbiology and Biomedical Genomics Center, University of Minnesota, St Paul, Minnesota 55108, USA
J Clin Microbiol 42:1694-702. 2004..Taken together, the results of our studies suggest that MLSSR sequencing enables facile and reproducible high-resolution subtyping of M. paratuberculosis isolates for molecular epidemiologic and population genetic analyses...
- Evaluation of a Pourquier ELISA kit in relation to agar gel immunodiffusion (AGID) test for assessment of the humoral immune response in sheep and goats with and without Mycobacterium paratuberculosis infectionS Gumber
Faculty of Veterinary Science, The University of Sydney, Private Bag 3, Camden 2570, NSW, Australia
Vet Microbiol 115:91-101. 2006..0% were observed for positive and negative controls included on 193 plates over a 10-month period and there was a high level of intraassay repeatability with 12.0% of the duplicate samples having CV of >15.0%...
- Paratuberculosis in farmed and free-living wild ruminants in the Czech Republic (1999-2001)M Machackova
Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
Vet Microbiol 101:225-34. 2004..We found that non-vertebrates, wild ruminants or non-ruminant wildlife can be vectors and potentially become a risk factor in the spread of M. a. paratuberculosis infection...
- Comparison of the proteosomes and antigenicities of secreted and cellular proteins produced by Mycobacterium paratuberculosisDonghee Cho
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, 2015 Linden Drive, Madison, WI 53706, USA
Clin Vaccine Immunol 13:1155-61. 2006..expression profiles and antigenicities of both culture filtrates (CF) and cellular extracts (CE) of Mycobacterium paratuberculosis were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), one-dimensional ..
- Application of the genome sequence to address concerns that Mycobacterium avium subspecies paratuberculosis might be a foodborne pathogenJohn P Bannantine
USDA ARS National Animal Disease Center, Ames, Iowa 50010, USA
Foodborne Pathog Dis 1:3-15. 2004..Application of these new tools in addressing foodborne related issues of M. paratuberculosis is discussed...
- Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis: evidence for limited strain diversity, strain sharing, and identification of unique targets for diagnosisAlifiya S Motiwala
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, 44691, USA
J Clin Microbiol 41:2015-26. 2003..Further, the results of our analyses reveal a relatively higher degree of genetic heterogeneity among M. avium subsp. paratuberculosis isolates recovered from human and ovine sources...
- Evaluation of bacteriologic culture of pooled fecal samples for detection of Mycobacterium paratuberculosisScott J Wells
College of Veterinary Medicine, Department of Clinical and Population Sciences, University of Minnesota, St Paul 55108 1004, USA
Am J Vet Res 63:1207-11. 2002To compare sensitivity of several methods of bacteriologic culture of pooled bovine fecal samples for detection of Mycobacterium paratuberculosis and evaluate homogeneity in number of M paratuberculosis in pooled fecal samples.
- Survival and dormancy of Mycobacterium avium subsp. paratuberculosis in the environmentRichard J Whittington
Faculty of Veterinary Science, The University of Sydney, Sydney, Australia
Appl Environ Microbiol 70:2989-3004. 2004..However, survival of M. avium subsp. paratuberculosis in the environment is finite, consistent with its taxonomic description as an obligate parasite of animals...
- Mycobacterium paratuberculosis, Mycobacterium smegmatis, and lipopolysaccharide induce different transcriptional and post-transcriptional regulation of the IRG1 gene in murine macrophagesTina Basler
Institut fuer Mikrobiologie, Zentrum fuer Infektionsmedizin, Stiftung Tieraerztliche Hochschule Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany
J Leukoc Biol 79:628-38. 2006..Thus, our data demonstrate that transcriptional and post-transcriptional mechanisms are responsible for a differential IRG1 gene expression in murine macrophages treated with LPS, MAP, and M. smegmatis...
- Rapid and reliable method for quantification of Mycobacterium paratuberculosis by use of the BACTEC MGIT 960 systemSung Jae Shin
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, WI 53706 1102, USA
J Clin Microbiol 45:1941-8. 2007A simple method for the enumeration of viable Mycobacterium paratuberculosis cells was developed and evaluated using the MGIT 960 culture system. For each of 12 M...
- Identification of novel virulence determinants in Mycobacterium paratuberculosis by screening a library of insertional mutantsSung Jae Shin
Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581, USA
Infect Immun 74:3825-33. 2006Johne's disease, caused by Mycobacterium paratuberculosis infection, is a worldwide problem for the dairy industry and has a possible involvement in Crohn's disease in humans...
- Evaluation of bacteriologic culture of individual and pooled fecal samples for detection of Mycobacterium paratuberculosis in dairy cattle herdsScott J Wells
Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA
J Am Vet Med Assoc 223:1022-5. 2003To determine the sensitivity of bacteriologic culture of pooled fecal samples in detecting Mycobacterium paratuberculosis, compared with bacteriologic culture of individual fecal samples in dairy cattle herds.
- Prevalence of and risk factors for paratuberculosis in purebred beef cattleAllen J Roussel
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843, USA
J Am Vet Med Assoc 226:773-8. 2005..To estimate the prevalence of paratuberculosis in purebred beef cattle in Texas and identify risk factors for seropositivity...
- Rapid and sensitive detection of Mycobacterium avium subsp. paratuberculosis in bovine milk and feces by a combination of immunomagnetic bead separation-conventional PCR and real-time PCRSangeeta Khare
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843 4467, USA
J Clin Microbiol 42:1075-81. 2004....
- Survival of Mycobacterium avium subsp. paratuberculosis in dam water and sedimentRichard J Whittington
Faculty of Veterinary Science, The University of Sydney, Private Bag 3, Camden, NSW 2570, Australia
Appl Environ Microbiol 71:5304-8. 2005..Survival of the organism in water destined for human consumption will need to be addressed if the organism is found to be involved in the etiology of Crohn's disease...
- PCR detection of Mycobacterium paratuberculosis in Crohn's disease granulomas isolated by laser capture microdissectionP Ryan
Department of Medicine, National University of Ireland, Cork, Ireland
Gut 51:665-70. 2002....
- Development and application of quantitative polymerase chain reaction assay based on the ABI 7700 system (TaqMan) for detection and quantification of Mycobacterium avium subsp. paratuberculosisSung G Kim
Department of Population Medicine and Diagnostic Science, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853, USA
J Vet Diagn Invest 14:126-31. 2002..IS900 TaqMan could be a useful tool for determining viable cell counts by measuring changes in cell numbers over the incubation period...
- Variation in resistance of Mycobacterium paratuberculosis to acid environments as a function of culture mediumNackmoon Sung
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Appl Environ Microbiol 69:6833-40. 2003Acid resistance of Mycobacterium paratuberculosis was examined as a function of growth conditions (i.e., in vitro growth medium and pH). M...
- Detection of Mycobacterium avium subspecies paratuberculosis in Swiss dairy cattle by real-time PCR and culture: a comparison of the two assaysK Bögli-Stuber
Laboratories of the Swiss Federal Veterinary Office, Schwarzenburgstrasse 161, CH 3003 Bern, Switzerland
J Appl Microbiol 99:587-97. 2005..To compare the two different diagnostic assays for the detection of Mycobacterium avium ssp. paratuberculosis, the aetiological agent of paratuberculosis...
- An IS900-like sequence found in a Mycobacterium sp. other than Mycobacterium avium subsp. paratuberculosisStina Englund
Department of Bacteriology, National Veterinary Institute, SE 751 89 Uppsala, Sweden
FEMS Microbiol Lett 209:267-71. 2002..Strong amplifications were obtained with several PCR primers described for detection of IS900. This finding shows the need of alternative PCR systems based on other genes than IS900 to confirm the presence of M. paratuberculosis...
- Incidence of Mycobacterium paratuberculosis in bulk raw and commercially pasteurized cows' milk from approved dairy processing establishments in the United KingdomIrene R Grant
Department of Food Science, Food Microbiology, Queen s University of Belfast, Northern Ireland, United Kingdom
Appl Environ Microbiol 68:2428-35. 2002..approved dairy processing establishments throughout the United Kingdom were tested for the presence of Mycobacterium paratuberculosis by immunomagnetic PCR (to detect all cells living and dead) and culture (to detect viable cells)...
- Development and evaluation of a Mycobacterium avium subspecies paratuberculosis (MAP) specific multiplex PCR assayT Tasara
Institute of Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Winterthurerstr 272, CH 8057 Zurich, Switzerland
Int J Food Microbiol 104:279-87. 2005..Finally, the potential application of this multiplex PCR system in milk analysis is demonstrated through analysis of raw milk samples artificially contaminated with MAP...
- Genome scale comparison of Mycobacterium avium subsp. paratuberculosis with Mycobacterium avium subsp. avium reveals potential diagnostic sequencesJohn P Bannantine
National Animal Disease Center, Agricultural Research Service, U S Department of Agriculture, Ames, Iowa 50010, USA
J Clin Microbiol 40:1303-10. 2002..avium subsp. paratuberculosis. Collectively, these studies suggest that a genomics approach will help in identifying novel M. avium subsp. paratuberculosis genes as candidate diagnostic sequences...
- Paratuberculosis with special reference to cattle. A reviewI Olsen
National Veterinary Institute, Oslo, Norway
Vet Q 24:12-28. 2002..Special emphasis will be on the available diagnostic methods, their use and limitations...
- Characterization of novel coding sequences specific to Mycobacterium avium subsp. paratuberculosis: implications for diagnosis of Johne's DiseaseMichael L Paustian
National Animal Disease Center, USDA ARS, 2300 North Dayton Ave, Ames, IA 50010, USA
J Clin Microbiol 42:2675-81. 2004..avium subsp. paratuberculosis-infected cattle. Collectively, the results of this study demonstrate the utility of genomic data to identify potential diagnostic sequences...
- Newly developed primers for the detection of Mycobacterium avium subspecies paratuberculosisElke Vansnick
Veterinary Department, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium
Vet Microbiol 100:197-204. 2004..The combination of the two PCR assays has proven to be useful for the identification of Map but validation on a large range of clinical samples still needs to be done...
- Gene expression profiling of peripheral blood mononuclear cells from cattle infected with Mycobacterium paratuberculosisPaul M Coussens
Department of Animal Science and Center for Animal Functional Genomics, Michigan State University, East Lansing, Michigan 48824, USA
Infect Immun 70:5494-502. 2002..Stimulation of PBMCs from clinically infected cows with Mycobacterium paratuberculosis tended to decrease expression of 83 genes (fold change, >1.5)...
- Efficacy of commercial and field-strain Mycobacterium paratuberculosis vaccinations with recombinant IL-12 in a bovine experimental infection modelJude E Uzonna
Department of Clinical Studies, University of Pennsylvania School of Veterinary Medicine, New Bolton Center, 382 West Street Road, 19348, Kennett Square, PA, USA
Vaccine 21:3101-9. 2003..The effect of prior exposure to Mycobacterium paratuberculosis and the adjuvant effect of rIL-12 on vaccine efficacy were also tested...
- Surveillance of bulk raw and commercially pasteurized cows' milk from approved Irish liquid-milk pasteurization plants to determine the incidence of Mycobacterium paratuberculosisCiara E O'Reilly
Food Safety Authority of Ireland, Abbey Court, Lower Abbey St, Dublin 1, Ireland
Appl Environ Microbiol 70:5138-44. 2004..n = 389) and commercially pasteurized (n = 357) liquid-milk supplies to determine the incidence of Mycobacterium paratuberculosis. The pasteurization time-temperature conditions were recorded for all pasteurized samples...
- Genetic diversity of Mycobacterium avium subspecies paratuberculosis isolates from goats detected by pulsed-field gel electrophoresisL De Juan
Moredun Research Institute, Pentlands Science Park, Penicuik EH26 OPZ, Scotland, UK
Vet Microbiol 106:249-57. 2005..All isolates were genotyped as Type II strains, except two isolates that resembled the intermediate group referred as Type III...
- Immunogenicity of a 16.7 kDa Mycobacterium paratuberculosis antigenJacob Mullerad
Department of Clinical Microbiology, Faculty of Medicine, Hadassah Medical School, The Hebrew University, P O Box 12272, Jerusalem, Israel
Microb Pathog 34:81-90. 2003b>Mycobacterium paratuberculosis (MPT), the agent of paratuberculosis is a slow growing mycobacteria that causes important economic losses mainly due to lower weight gains and drastic decrease in milk production...
- Real-time PCR-based methods for detection of Mycobacterium avium subsp. paratuberculosis in water and milkDavid Rodríguez-Lázaro
Institute of Food and Agricultural Technology INTEA, University of Girona, Spain
Int J Food Microbiol 101:93-104. 2005..avium paratuberculosis in 20 ml artificially contaminated semi-skimmed milk. The assay will be a useful addition to the range of diagnostic tools available for the study of M. avium paratuberculosis...
- Use of multilocus variable-number tandem-repeat analysis for typing Mycobacterium avium subsp. paratuberculosisPieter Overduin
Mycobacteria Reference Laboratory, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
J Clin Microbiol 42:5022-8. 2004..MLVA typing subdivided the most predominant RFLP type, R01, into six subtypes and thus provides a promising molecular subtyping approach to study the diversity of M. avium subsp. paratuberculosis...
- Certification of herds as free of Mycobacterium paratuberculosis infection: actual pooled faecal results versus certification model predictionsC H J Kalis
Animal Health Service GDltd, PO Box 9, 7400 AA Deventer, The Netherlands
Prev Vet Med 65:189-204. 2004..One hundred dairy herds were tested for Mycobacterium paratuberculosis at 6-month intervals by pooled faecal culture (five individual animal samples per pool) with solid ..
- Detection and Isolation of Mycobacterium avium subspecies paratuberculosis from intestinal mucosal biopsies of patients with and without Crohn's disease in SardiniaLeonardo A Sechi
Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Universita degli Studi di Sassari, Sassari, Italy
Am J Gastroenterol 100:1529-36. 2005..The present study was designed to determine what proportion of people in Sardinia attending for ileocolonoscopy with or without Crohn's disease were infected with this pathogen...
- Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis isolates recovered from wild animal speciesAlifiya S Motiwala
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster, Ohio 44691, USA
J Clin Microbiol 42:1703-12. 2004..paratuberculosis isolates from different host species and provide evidence for the host specificity of some M. paratuberculosis strains as well as sharing of strains between wild and domesticated animal species...
- Detection and verification of Mycobacterium avium subsp. paratuberculosis in fresh ileocolonic mucosal biopsy specimens from individuals with and without Crohn's diseaseTim J Bull
Department of Surgery, St George s Hospital Medical School, London SW17 0RE, United Kingdom
J Clin Microbiol 41:2915-23. 2003..avium subsp. paratuberculosis was verified by amplicon sequencing. The rate of detection of M. avium subsp. paratuberculosis in individuals with CD is highly significant and implicates this chronic enteric pathogen in disease causation...
- Rapid real-time PCR assay for detection and quantitation of Mycobacterium avium subsp. paratuberculosis DNA in artificially contaminated milkJim O'Mahony
Microbiology Department and Alimentary Pharmabiotic Centre, National University of Ireland, Cork, Ireland
Appl Environ Microbiol 70:4561-8. 2004..Furthermore, the entire procedure (extraction and PCR) was performed in less than 3 h and was successfully adapted to quantify M. avium subsp. paratuberculosis in spiked milk from heavily and mildly contaminated samples...
- Effect of commercial-scale high-temperature, short-time pasteurization on the viability of Mycobacterium paratuberculosis in naturally infected cows' milkIrene R Grant
Department of Food Science Food Microbiology, Queen s University of Belfast, Newforge Lane, Belfast BT9 5PX, N Ireland, UK
Appl Environ Microbiol 68:602-7. 2002Raw cows' milk naturally infected with Mycobacterium paratuberculosis was pasteurized with an APV HXP commercial-scale pasteurizer (capacity 2,000 liters/h) on 12 separate occasions...
- Comparison of four different culture media for isolation and growth of type II and type I/III Mycobacterium avium subsp. paratuberculosis strains isolated from cattle and goatsLucia de Juan
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda Puerta de Hierro s n, 28040 Madrid, Spain
Appl Environ Microbiol 72:5927-32. 2006....
- Replication and long-term persistence of bovine and human strains of Mycobacterium avium subsp. paratuberculosis within Acanthamoeba polyphagaManuela Mura
Department of Cardiac and Vascular Sciences Surgery, St George s University of London, London SW17 0RE, United Kingdom
Appl Environ Microbiol 72:854-9. 2006..Control cultures were negative. M. avium subsp. paratuberculosis has the potential for long-term persistence in environmental protists...
- Effect of Mycobacterium paratuberculosis infection on production, reproduction, and health traits in US HolsteinsM G Gonda
Dairy Science Department, University of Wisconsin, 1675 Observatory Drive, Madison, WI 53706, United States
Prev Vet Med 80:103-19. 2007Our objective was to estimate the effect of Mycobacterium paratuberculosis infection on milk, fat, and protein yield deviations, pregnancy rate, lactation somatic cell score, and projected total months in milk (productive life)...
- Mycobacterium avium ssp. paratuberculosis and its potential survival tacticsM T Rowe
Food Microbiology Branch, Agriculture, Food and Environmental Science Division, Department of Agriculture and Rural Development for Northern Ireland, Belfast, UK
Lett Appl Microbiol 42:305-11. 2006..It then focuses on the potential mechanisms Map might employ to survive and disseminate in the environment, including interaction with protozoa and insects, dormancy, biofilm formation and aerosolization...
- Application of an F57 sequence-based real-time PCR assay for Mycobacterium paratuberculosis detection in bulk tank raw milk and slaughtered healthy dairy cowsC Bosshard
Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, CH 8057 Zurich, Switzerland
J Food Prot 69:1662-7. 2006..9% (5 of 101) in mesenteric lymph nodes, 0.9% (1 of 101) in ileum tissue, and 3.6% (3 of 84) in milk. Meanwhile, for 2.9% (3 of 101) of the culled cows, MAP DNA was detected in samples of diaphragmatic muscles...
- Molecular typing of Mycobacterium avium subspecies paratuberculosis strains from different hosts and regionsI x Sevilla
Instituto Vasco de Investigacion y Desarrollo Agrario NEIKER, Berreaga 1, 48160 Derio, Bizkaia, Spain
Rev Sci Tech 24:1061-6. 2005..These results show that there are genetic differences between Map isolates related to geographic and host factors that have a potential use in the epidemiological tracing of new paratuberculosis isolates...
- Epitope shared by functional variant of organic cation/carnitine transporter, OCTN1, Campylobacter jejuni and Mycobacterium paratuberculosis may underlie susceptibility to Crohn's disease at 5q31Anne Marie Lamhonwah
Division of Neurology, Department of Pediatrics, The Hospital for Sick Children, Toronto, Ont, Canada
Biochem Biophys Res Commun 337:1165-75. 2005Campylobacter jejuni and Mycobacterium paratuberculosis have been implicated in the pathogenesis of Crohn's disease...
- Mycobacterium paratuberculosis and milkI R Grant
Department of Food Science Microbiology, Queen s University, Belfast, N Ireland, UK
Acta Vet Scand 44:261-6. 2003..Hence, both raw and pasteurised cows' milk are potential vehicles of transmission of M. paratuberculosis to humans...
- Sensitive detection of Mycobacterium avium subsp. paratuberculosis in bovine semen by real-time PCRD Herthnek
National Veterinary Institute SVA, Uppsala, Sweden
J Appl Microbiol 100:1095-102. 2006..To develop a fast and sensitive protocol for detection of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine semen and to make a critical evaluation of the analytical sensitivity...
- Population-based case control study of seroprevalence of Mycobacterium paratuberculosis in patients with Crohn's disease and ulcerative colitisCharles N Bernstein
Inflammatory Bowel Disease Clinical and Research Centre, Department of Internal Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
J Clin Microbiol 42:1129-35. 2004There is renewed enthusiasm for exploring the possibility that Mycobacterium paratuberculosis may be causative in Crohn's disease (CD). We aimed to determine whether CD subjects are more likely to be M...
- Molecular epidemiology of Types I/III strains of Mycobacterium avium subspecies paratuberculosis isolated from goats and cattleL De Juan
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda Puerta de Hierro s n, 28040 Madrid, Spain
Vet Microbiol 115:102-10. 2006..The majority of these animals had not been in direct or indirect contact with sheep flocks infected with M. a. paratuberculosis. This fact should be taken into account when implementing paratuberculosis control programs...
- Johne's disease, inflammatory bowel disease, and Mycobacterium paratuberculosisOfelia Chacon
Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska 68583 090, USA
Annu Rev Microbiol 58:329-63. 2004..In addition, the experimental evidence defining MAP as the cause of Johne's disease and the issues and controversies surrounding its potential pathogenic role in humans are discussed...
- Effect of pasteurization on survival of Mycobacterium paratuberculosis in milkA Gao
Laboratory Services Division, University of Guelph, Guelph, Ontario N1H 8J7, Canada
J Dairy Sci 85:3198-205. 2002b>Mycobacterium paratuberculosis (Mptb) is the causative agent of Johne's disease of ruminant animals including cattle, goats, and sheep...
- The complete genome sequence of Mycobacterium avium subspecies paratuberculosisLingling Li
Department of Microbiology, University of Minnesota, St Paul, MN 55108, USA
Proc Natl Acad Sci U S A 102:12344-9. 2005....
- The immunogenicity of Mycobacterium paratuberculosis 85B antigenJacob Mullerad
Department of Clinical Microbiology, Hadassah Medical School, The Hebrew University, Jerusalem, Israel
Med Microbiol Immunol 190:179-87. 2002b>Mycobacterium paratuberculosis (MPT) is the etiological agent of paratuberculosis. The disease is prevalent throughout the world, and exacts a heavy financial toll...
- IS900 restriction fragment length polymorphism (RFLP) analysis of Mycobacterium avium subsp. paratuberculosis isolates from goats and cattle in NorwayB Djønne
Department of Bacteriology, National Veterinary Institute, Post Box 8156 Dep, N 0033 Oslo, Norway
Acta Vet Scand 46:13-8. 2005..This lack of difference indicates that the most common M. a. paratuberculosis isolates in Norway may infect both cattle and goats...
- An estimated prevalence of Johne's disease in a subpopulation of Alabama beef cattleB B Hill
Department of Pathobiology, Auburn University College of Veterinary Medicine, Auburn, AL 36849, USA
J Vet Diagn Invest 15:21-5. 2003..avium ssp. Paratuberculosis, and the overall prevalence of infection in Alabama beef cattle is approximately 8%, which correlates with other previously published regional estimates...
- Immune responses after oral inoculation of weanling bison or beef calves with a bison or cattle isolate of Mycobacterium avium subsp. paratuberculosisJ R Stabel
USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, 2300 Dayton Road, Ames, Iowa 50010, USA
J Wildl Dis 39:545-55. 2003..Antibody was not detected in either beef or bison calves during the 6 mo infection period. These results indicate that the host response to strains of M. avium subsp. paratuberculosis may differ between ruminant species...
- Factors affecting the herd level of antibodies against Mycobacterium avium subspecies paratuberculosis in dairy cattleB Fredriksen
Department of Epidemiology, National Veterinary Institute, PO Box 8156, Dep N 0033 Oslo, Norway
Vet Rec 154:522-6. 2004..However, diagnostic tests showed that none of the animals in the case herds was infected with M a paratuberculosis...
- Antigenicity of Mycobacterium paratuberculosis superoxide dismutase in miceJacob Mullerad
Department of Clinical Microbiology, The Hebrew University, Hadassah Medical School, P O B 12272, Jerusalem, Israel
FEMS Immunol Med Microbiol 34:81-8. 2002b>Mycobacterium paratuberculosis (MPT) is the etiologic agent of paratuberculosis. The disease is prevalent in cattle worldwide, and exacts a heavy financial toll...
- Use of growth indices from radiometric culture for quantification of sheep strains of Mycobacterium avium subsp. paratuberculosisLeslie A Reddacliff
Faculty of Veterinary Science, University of Sydney, Sydney, New South Wales 2570, Australia
Appl Environ Microbiol 69:3510-6. 2003..Organisms from feces or contaminated soil grew more slowly than those from cultures or tissues, and separate equations were developed for enumeration from these sources...
- Assessment of management-related risk factors for paratuberculosis in Danish dairy herds using Bayesian mixture modelsS S Nielsen
Department of Large Animal Sciences, Faculty of Life Science, University of Copenhagen, Grønnegårdsvej 8, DK 1870 Frederiksberg C, Denmark
Prev Vet Med 81:306-17. 2007..Surprisingly, the hygiene level in the calving area was not found to affect the odds of infection...
- Simulated economic effects of improving the sensitivity of a diagnostic test in paratuberculosis controlAnne Braad Kudahl
Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, Foulum, Denmark
Prev Vet Med 78:118-29. 2007..Also, the milk-ELISA test with its current Se seemed to be profitable already after 2 years in high-prevalence herds using a test-&-cull strategy based on the milk-ELISA alone...
- Longitudinal study of interferon-gamma, serum antibody and milk antibody responses in cattle infected with Mycobacterium avium subsp. paratuberculosisA Huda
Danish Institute for Food and Veterinary Research, Bulowsvej 27, DK 1790 Copenhagen V, Denmark
Vet Microbiol 104:43-53. 2004..In conclusion, the IFN-gamma test may be applied for screening of cattle of 1 and 2 years of age for exposure to M. paratuberculosis and the antibody ELISAs from 3 years of age...
- Comparison of milk and serum enzyme-linked immunosorbent assays for diagnosis of Mycobacterium avium subspecies paratuberculosis infection in dairy cattleJason E Lombard
Integrated Livestock Management, Department of Clinical Sciences, Colorado State University, Fort Collins, CO, USA
J Vet Diagn Invest 18:448-58. 2006..At the cow and herd levels, milk ELISA performed equivalent to serum ELISA using fecal culture as a reference for MAP infection and has the advantage of decreased labor costs on farms that use Dairy Herd Improvement Association testing...
- Association of fecal shedding of mycobacteria with high ELISA-determined seroprevalence for paratuberculosis in beef herdsAllen J Roussel
Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843, USA
J Am Vet Med Assoc 230:890-5. 2007..To evaluate the seroprevalence of paratuberculosis by use of 2 commercial ELISAs in association with prevalence of fecal shedding of mycobacteria within beef cattle herds...
- Serological, bacteriological and molecularbiological survey of paratuberculosis (Johne's disease) in Austrian cattleS Dreier
Clinic for Ruminants, Department for Farm Animals and Herd Management, University of Veterinary Medicine Vienna, Veterinarplatz 1, 1210 Vienna, Austria
J Vet Med B Infect Dis Vet Public Health 53:477-81. 2006..When this study is compared with a similar study performed in Austria 1999, a significant increase of positive cattle and farms could be seen in Austria...
- Comparison between a commercial and an in-house ELISA or anti-M. avium paratuberculosis antibodies detection in dairy herds in Rio de Janeiro, BrazilRachel Ferreira
Instituto de Microbiologia Prof Paulo de Góes Departamento de Microbiologia Medica, Universidade Federal do Rio de Janeiro
Rev Latinoam Microbiol 44:129-32. 2002..320. PPA-ELISA can be useful as a collective herd test and can be recommended as a screening test for identification of infected herds and foci of Johne's disease...
- Field testing of an enhanced direct-fecal polymerase chain reaction procedure, bacterial culture of feces, and a serum enzyme-linked immunosorbent assay for detecting Mycobacterium avium subsp paratuberculosis infection in adult dairy cattleH Morgan Scott
Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843, USA
Am J Vet Res 68:236-45. 2007....
- Optimization of methods for detecting Mycobacterium avium subsp. paratuberculosis in environmental samples using quantitative, real-time PCRKimberly L Cook
AWMRU, USDA ARS, 230 Bennett Lane, Bowling Green, KY 42104, USA
J Microbiol Methods 69:154-60. 2007..paratuberculosis concentrations in environmental samples...
- [The suitability of ELISA for the detection of antibodies against Mycobacterium avium ssp. paratuberculosis in bulk milk samples from Rhineland-Palatinate]Lutz Geue
Friederich Loeffler Institut, Bundesforschungsinstiut fũr Tiergesundheit, Institut fur Epidemiologie, Wusterhausen
Berl Munch Tierarztl Wochenschr 120:67-78. 2007....
- Detection of Mycobacterium avium subspecies paratuberculosis in Swiss dairy cattle by culture and serologyB Glanemann
Institute of Veterinary Bacteriology, University of Zurich, Switzerland
Schweiz Arch Tierheilkd 146:409-15. 2004..The percentage of ELISA-positive cows was 16.8%. Overall agreement between antibody detection and MAP-positive faecal culture was 15.4%...
- Nested PCR on blood and milk for the detection of Mycobacterium avium subsp paratuberculosis DNA in clinical and subclinical bovine paratuberculosisC D Buergelt
Department of Pathobiology, College of Veterinary Medicine, University of Florida, PO Box 110880, Gainesville, Florida 32610, USA
Aust Vet J 82:497-503. 2004..To determine the potential of PCR on blood and milk to detect cattle infected with Mycobacterium avium subsp paratuberculosis...
- Efficacy of immunologic assays for the detection of Johne's disease in dairy cows fed additional energy during the periparturient periodJudith R Stabel
USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, Ames, IA 50010, USA
J Vet Diagn Invest 16:412-20. 2004..Twelve dairy cows naturally infected with Mycobacterium paratuberculosis were fitted with rumen cannulas in late gestation and assigned to treatment groups: control, n = 6; or ..
- Evaluation of highly sensitive indigenous milk ELISA kit with fecal culture, milk culture and fecal-PCR for the diagnosis of bovine Johne's disease (BJD) in IndiaS V Singh
Goat Health Division, Central Institute for Research on Goats, Makhdoom, P O Farah, Mathura, UP 281 122, India
Comp Immunol Microbiol Infect Dis 30:175-86. 2007....
- Evaluation of enzyme-linked immunosorbent assays performed on milk and serum samples for detection of paratuberculosis in lactating dairy cowsSteven H Hendrick
Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada
J Am Vet Med Assoc 226:424-8. 2005..To determine whether results obtained for milk and serum samples with ELISAs intended for diagnosis of paratuberculosis in dairy cows were comparable to results obtained by means of mycobacterial culture of fecal samples...
- Use of age and milk production data to improve the ability of enzyme-linked immunosorbent assay test results to predict Mycobacterium avium ssp. paratuberculosis fecal culture statusRoy D Berghaus
Department of Population Health and Reproduction School of Veterinary Medicine, University of California, Davis, CA 95616, USA
J Vet Diagn Invest 18:233-42. 2006..For a given ELISA S/P ratio, older cows and those with lower 305 ME milk production relative to other cows in the herd were significantly more likely to be shedding MAP in their feces at the end of lactation...
- Environmental sampling for detection of Mycobacterium avium ssp. paratuberculosis on large California dairiesR D Berghaus
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, 95616, USA
J Dairy Sci 89:963-70. 2006..Environmental sampling was an effective and inexpensive method of identifying herds infected with Mycobacterium avium ssp. paratuberculosis...
- The prevalence of milk and serum antibodies to Mycobacterium avium subspecies paratuberculosis in dairy herds in OntarioSteven Hendrick
Department of Population Medicine, University of Guelph, Guelph, Ontario N1G 2W1
Can Vet J 46:1126-9. 2005..The apparent cow level prevalence was 1.7% and 2.6% on the milk and serum ELISA, respectively. The serum and milk assays agreed moderately...
- Risk of removal and effects on milk production associated with paratuberculosis status in dairy cowsJason E Lombard
USDA Animal and Plant Health Inspection Service, Centers for Epidemiology and Animal Health, 2150 Centre Ave, Bldg B 2E7, Fort Collins, CO 80526, USA
J Am Vet Med Assoc 227:1975-81. 2005..To determine effects on production and risk of removal related to Mycobacterium avium subsp paratuberculosis (MAP) infection at the individual animal level in dairy cattle...
- Evaluation of IS900-PCR assay for detection of Mycobacterium avium subspecies Paratuberculosis infection in cattle using quarter milk and bulk tank milk samplesB M Jayarao
Department of Veterinary Science, Pennsylvania State University, University Park 16802, USA
Foodborne Pathog Dis 1:17-26. 2004..avium subsp. paratuberculosis infection...
- New method of serological testing for Mycobacterium avium subsp. paratuberculosis (Johne's disease) by flow cytometryS Eda
Center for Wildlife Health, Department of Forestry, Wildlife and Fisheries, University of Tennessee, Knoxville, TN 37996, USA
Foodborne Pathog Dis 2:250-62. 2005..Moreover, the FCM is objective, technically easy and can be automated for handling large numbers of samples. This novel assay might form the basis of a highly sensitive and subspecies-specific test for the diagnosis of JD...
- Development of a nested PCR method targeting a unique multicopy element, ISMap02, for detection of Mycobacterium avium subsp. paratuberculosis in fecal samplesJ R Stabel
USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, Ames, IA 50010, USA
J Clin Microbiol 43:4744-50. 2005..In conclusion, the ISMap02 element provides a very sensitive and specific alternative as a diagnostic reagent for use in PCR assays for the detection of paratuberculosis...
- Evaluation of three ELISAs for Mycobacterium avium subsp. paratuberculosis using tissue and fecal culture as comparison standardsS L B McKenna
University of Prince Edward Island, Atlantic Veterinary College, Department of Health Managament, 550 University Avenue, Charlottetown, PEI, Canada C1A 4P3
Vet Microbiol 110:105-11. 2005..529. Overall, the sensitivities of the ELISAs when compared to tissue culture were low. The apparent advantage of the unabsorbed ELISA with respect to sensitivity is at the cost of lowered specificity and test accuracy...
- [Observations on the control and eradication of paratuberculosis in dairy herds]J Stratmann
Institut fur Mikrobiologie, Zentrum für Infektionsmedizin, Stiftung Tierarztliche Hochschule Hannover
Dtsch Tierarztl Wochenschr 112:304-6. 2005....
- Longitudinal study of ELISA seroreactivity to Mycobacterium avium subsp. paratuberculosis in infected cattle and culture-negative herd matesRaymond W Sweeney
Department of Clinical Studies New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square 19348, USA
J Vet Diagn Invest 18:2-6. 2006..In this study, change in S/P was not a useful predictor of infection status in seronegative cows...
- Genetic variation of Mycobacterium avium ssp. paratuberculosis infection in US HolsteinsM G Gonda
Dairy Science Department, University of Wisconsin, Madison 53706, USA
J Dairy Sci 89:1804-12. 2006..183, respectively), with a corresponding increase in precision (PSD = 0.096 and 0.082, respectively). This study demonstrates that exploitable genetic variation exists in dairy cattle for M. paratuberculosis infection susceptibility...
- Monensin might protect Ontario, Canada dairy cows from paratuberculosis milk-ELISA positivitySteven H Hendrick
Department of Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
Prev Vet Med 76:237-48. 2006..54). Monensin use might be associated with milk-ELISA positivity, but its impact on the transmission of paratuberculosis remains unknown...
- Detection of Mycobacterium avium subsp. paratuberculosis in bovine manure using Whatman FTA card technology and Lightcycler real-time PCRCarmela V Jaravata
Dairy Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, California 95616, USA
Foodborne Pathog Dis 3:212-5. 2006..This cost-efficient assay allows reporting of results as early as 4 h after fecal collection, which can be particularly useful in highthroughput herd screening...
- Time delay, temperature effects and assessment of positive controls on whole blood for the gamma interferon ELISA to detect paratuberculosisS Robbe-Austerman
National Animal Disease Center, Agricultural Research Service, USDA, 2300 Dayton Ames, IA 50010, USA
J Vet Med B Infect Dis Vet Public Health 53:213-7. 2006..The PPDs were more potent at stimulating IFN-gamma production than the whole cell sonicate. In conclusion, whole blood should be stored/transported at ambient room temperature and stimulated within 12 h of collection...
- A long-term study in Merino sheep experimentally infected with Mycobacterium avium subsp. paratuberculosis: clinical disease, faecal culture and immunological studiesD J Stewart
CSIRO Livestock Industries, Australian Animal Health Laboratory, Private Bag 24, Geelong 3220, Victoria, Australia
Vet Microbiol 104:165-78. 2004..The results indicated that for sheep the interferon-gamma test may be useful for determining if a flock has been exposed to ovine Johne's disease...
- Molecular Definition of Mycobacterium paratuberculosisJULIA INAMINE; Fiscal Year: 2005..paratuberculosis to maintain a specific biological niche that is not shared by the closely related M. avium subspecies avium. ..
- Mycobacterium avium subspecies in Crohn's DiseaseSaleh Naser; Fiscal Year: 2005..Ultimately, the outcome of this study will go a long way toward clearing up the MAP debate. ..
- HUMAN EHRLICHIOSIS SURVEILLANCE AND EPIDEMIOLOGYWilliam Davidson; Fiscal Year: 2003....
- Regulatory networks contributing to L. monocytogenes transmission and virulenceKathryn Boor; Fiscal Year: 2009..This knowledge will contribute to identification of specific bacterial mechanisms that can be targeted for development of novel and innovative bacterial control strategies, including new antibacterial therapeutics. ..
- Sigma B and stress response in Listeria monocytogenesKathryn Boor; Fiscal Year: 2006..More broadly, L. monocytogenes will serve as a model system for examining the role of alternative sigma factor-directed general stress response systems in survival and pathogenesis of gram-positive food-borne pathogens. ..
- Regulatory networks contributing to L. monocytogenes transmission and virulenceKathryn J Boor; Fiscal Year: 2010..This knowledge will contribute to identification of specific bacterial mechanisms that can be targeted for development of novel and innovative bacterial control strategies, including new antibacterial therapeutics. ..