thermoplasma

Summary

Summary: A genus of facultatively anaerobic heterotrophic archaea, in the order THERMOPLASMALES, isolated from self-heating coal refuse piles and acid hot springs. They are thermophilic and can grow both with and without sulfur.

Top Publications

  1. ncbi Crystal structure of the tricorn protease reveals a protein disassembly line
    H Brandstetter
    Nature 414:466-70. 2001
  2. pmc Domain architecture of the stator complex of the A1A0-ATP synthase from Thermoplasma acidophilum
    Erik Kish-Trier
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 284:12031-40. 2009
  3. ncbi Variation in molecular species of polar lipids from thermoplasma acidophilum depends on growth temperature
    I Uda
    Division of Chemistry, Center for Natural Science, Kitasato University, Sagamihara, Kanagawa, Japan
    Lipids 36:103-5. 2001
  4. ncbi Quantitative dynamics and binding studies of the 20S proteasome by NMR
    Remco Sprangers
    Department of Biochemistry, The University of Toronto, Toronto, Ontario M5S 1A8, Canada
    Nature 445:618-22. 2007
  5. ncbi Glyceraldehyde dehydrogenases from the thermoacidophilic euryarchaeota Picrophilus torridus and Thermoplasma acidophilum, key enzymes of the non-phosphorylative Entner-Doudoroff pathway, constitute a novel enzyme family within the aldehyde dehydrogenase s
    Matthias Reher
    Institut fur Allgemeine Mikrobiologie, Christian Albrechts Universitat Kiel, Am Botanischen Garten 1 9, D 24118 Kiel, Germany
    FEBS Lett 580:1198-204. 2006
  6. pmc Effects of pH and temperature on the composition of polar lipids in Thermoplasma acidophilum HO-62
    Haruo Shimada
    Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan
    J Bacteriol 190:5404-11. 2008
  7. ncbi The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum - recombinant expression, assembly and characterization
    Caroline Heath
    Centre for Extremophile Research, Department of Biology and Biochemistry, University of Bath, UK
    FEBS J 274:5406-15. 2007
  8. ncbi VAT, the thermoplasma homolog of mammalian p97/VCP, is an N domain-regulated protein unfoldase
    Alexandra Gerega
    Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    J Biol Chem 280:42856-62. 2005
  9. pmc Nuclear localization signals of human and Thermoplasma proteasomal alpha subunits are functional in vitro
    P M Nederlof
    Department of Structural Biology, Max Planck Institut fur Biochemie, Martinsried, Germany
    Proc Natl Acad Sci U S A 92:12060-4. 1995
  10. ncbi The Chaperones of the archaeon Thermoplasma acidophilum
    A Ruepp
    Department of Molecular Structural Biology, Max-Planck-Institute for Biochemistry, Am Klopferspitz 18a, Martinsried, D-82152, Germany
    J Struct Biol 135:126-38. 2001

Research Grants

Detail Information

Publications197 found, 100 shown here

  1. ncbi Crystal structure of the tricorn protease reveals a protein disassembly line
    H Brandstetter
    Nature 414:466-70. 2001
    ..These products need further processing. Recently, a proteolytic system was identified in the model organism Thermoplasma acidophilum that performs this processing...
  2. pmc Domain architecture of the stator complex of the A1A0-ATP synthase from Thermoplasma acidophilum
    Erik Kish-Trier
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 284:12031-40. 2009
    ..Previously, we have characterized the peripheral stalk forming subunits E and H of the A-ATPase from Thermoplasma acidophilum and demonstrated that the two polypeptides interact to form a stable heterodimer with 1:1 ..
  3. ncbi Variation in molecular species of polar lipids from thermoplasma acidophilum depends on growth temperature
    I Uda
    Division of Chemistry, Center for Natural Science, Kitasato University, Sagamihara, Kanagawa, Japan
    Lipids 36:103-5. 2001
    ..C40 isoprenoid chains, having different numbers of cyclopentane rings, were detected in the ether core lipid of Thermoplasma acidophilum...
  4. ncbi Quantitative dynamics and binding studies of the 20S proteasome by NMR
    Remco Sprangers
    Department of Biochemistry, The University of Toronto, Toronto, Ontario M5S 1A8, Canada
    Nature 445:618-22. 2007
    ..Our results establish that NMR spectroscopy can provide detailed insight into supra-molecular structures over an order of magnitude larger than those routinely studied using methodology that is generally applicable...
  5. ncbi Glyceraldehyde dehydrogenases from the thermoacidophilic euryarchaeota Picrophilus torridus and Thermoplasma acidophilum, key enzymes of the non-phosphorylative Entner-Doudoroff pathway, constitute a novel enzyme family within the aldehyde dehydrogenase s
    Matthias Reher
    Institut fur Allgemeine Mikrobiologie, Christian Albrechts Universitat Kiel, Am Botanischen Garten 1 9, D 24118 Kiel, Germany
    FEBS Lett 580:1198-204. 2006
    ..torridus as the encoding gene, designated gadh, and the recombinant GADH was characterized. In Thermoplasma acidophilum ORF Ta0809 represents a gadh homolog with highest sequence identity; the gene was expressed and the ..
  6. pmc Effects of pH and temperature on the composition of polar lipids in Thermoplasma acidophilum HO-62
    Haruo Shimada
    Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan
    J Bacteriol 190:5404-11. 2008
    b>Thermoplasma acidophilum HO-62 was grown at different pHs and temperatures, and its polar lipid compositions were determined. Although the number of cyclopentane rings in the caldarchaeol moiety increased when T...
  7. ncbi The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum - recombinant expression, assembly and characterization
    Caroline Heath
    Centre for Extremophile Research, Department of Biology and Biochemistry, University of Bath, UK
    FEBS J 274:5406-15. 2007
    ..In the current paper, we clone and express the four genes from the thermophilic archaeon, Thermoplasma acidophilum, and demonstrate that the recombinant enzymes are active and assemble into a large (M(r) = 5 x 10(6)..
  8. ncbi VAT, the thermoplasma homolog of mammalian p97/VCP, is an N domain-regulated protein unfoldase
    Alexandra Gerega
    Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    J Biol Chem 280:42856-62. 2005
    The Thermoplasma VCP-like ATPase from Thermoplasma acidophilum (VAT) ATPase is a member of the two-domain AAA ATPases and homologous to the mammalian p97/VCP and NSF proteins...
  9. pmc Nuclear localization signals of human and Thermoplasma proteasomal alpha subunits are functional in vitro
    P M Nederlof
    Department of Structural Biology, Max Planck Institut fur Biochemie, Martinsried, Germany
    Proc Natl Acad Sci U S A 92:12060-4. 1995
    ..proteasomal subunits (Hsc iota, Hsc9, and Hsc3), as well as a putative NLS-type sequence from the archaeon Thermoplasma acidophilum, for their ability to direct non-nuclear proteins to the nucleus...
  10. ncbi The Chaperones of the archaeon Thermoplasma acidophilum
    A Ruepp
    Department of Molecular Structural Biology, Max-Planck-Institute for Biochemistry, Am Klopferspitz 18a, Martinsried, D-82152, Germany
    J Struct Biol 135:126-38. 2001
    ..The archaeon most extensively used for such studies is Thermoplasma acidophilum, which thrives at 59 degrees C and pH 2...
  11. ncbi Cloning and overexpression of a thermostable signal peptide peptidase (SppA) from Thermoplasma volcanium GSS1 in E. coli
    Semra Kocabiyik
    Middle East Technical University, Department of Biological Sciences, Ankara, Turkey
    Biotechnol J 4:1055-65. 2009
    ..study, a gene coding for thermophilic serine protease of the ClpP class from the thermoacidophilic archaeon Thermoplasma volcanium (Tpv) was cloned and expressed in Escherichia coli...
  12. pmc The Thermoplasma acidophilum LplA-LplB complex defines a new class of bipartite lipoate-protein ligases
    Quin H Christensen
    Departments of Microbiology, Urbana, Illinois 61801, USA
    J Biol Chem 284:21317-26. 2009
    ..Two x-ray structures of the Thermoplasma acidophilum LplA structure have been reported, although the protein was reported to lack ligase activity...
  13. ncbi Cloning, sequencing and expression of VAT, a CDC48/p97 ATPase homologue from the archaeon Thermoplasma acidophilum
    V Pamnani
    Max Planck Institut fur Biochemie, Martinsried, Germany
    FEBS Lett 404:263-8. 1997
    A member of the AAA family of Mg2(+)-ATPases from the archaeon Thermoplasma acidophilum has been cloned and expressed in Escherichia coli...
  14. pmc Interaction of the Thermoplasma acidophilum A1A0-ATP synthase peripheral stalk with the catalytic domain
    Erik Kish-Trier
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
    FEBS Lett 583:3121-6. 2009
    ..The data show a specific interaction of B(NT) peptide with 26 residues of the E(CT1)H(CT) domain, thereby providing a molecular picture of how the peripheral stalk is anchored to the A3B3 catalytic domain in A1A0...
  15. pmc Complete polar lipid composition of Thermoplasma acidophilum HO-62 determined by high-performance liquid chromatography with evaporative light-scattering detection
    Haruo Shimada
    Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    J Bacteriol 184:556-63. 2002
    Polar ether lipids of Thermoplasma acidophilum HO-62 were purified by high-performance liquid chromatography with an evaporative light-scattering detector...
  16. pmc Identification and characterization of Thermoplasma acidophilum glyceraldehyde dehydrogenase: a new class of NADP+-specific aldehyde dehydrogenase
    Jin Hwa Jung
    Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja Dong, Pohang 790 784, Korea
    Biochem J 397:131-8. 2006
    Thermoacidophilic archaea such as Thermoplasma acidophilum and Sulfolobus solfataricus are known to metabolize D-glucose via the nED (non-phosphorylated Entner-Doudoroff) pathway...
  17. ncbi Thermal stability and biochemical properties of isocitrate dehydrogenase from the thermoacidophilic archaeon Thermoplasma acidophilum
    Runar Stokke
    Department of Biology, University of Bergen, Jahnebakken 5, P O Box 7800, 5020, Bergen, Norway
    Extremophiles 11:397-402. 2007
    Isocitrate dehydrogenase [IDH; EC 1.1.1.42] from the thermoacidophilic archaeon Thermoplasma acidophilum (TaIDH) showed high thermal stability with an apparent melting temperature, T(m), of 82.2 and 84.5 degrees C at pH 7.5 and 5...
  18. ncbi Bifunctional phosphoglucose/phosphomannose isomerases from the Archaea Aeropyrum pernix and Thermoplasma acidophilum constitute a novel enzyme family within the phosphoglucose isomerase superfamily
    Thomas Hansen
    Institut fur Allgemeine Mikrobiologie, Christian Albrechts Universitat Kiel, Am Botanischen Garten 1 9, D 24118 Kiel, Germany
    J Biol Chem 279:2262-72. 2004
    ..The homolog from Thermoplasma acidophilum (Ta1419) was overexpressed in E...
  19. ncbi The crystal structure of citrate synthase from the thermophilic archaeon, Thermoplasma acidophilum
    R J Russell
    School of Biology and Biochemistry, University of Bath, UK
    Structure 2:1157-67. 1994
    ..We have chosen citrate synthase, the first enzyme of the citric acid cycle, as a model enzyme for such studies...
  20. doi XPB helicase regulates DNA incision by the Thermoplasma acidophilum endonuclease Bax1
    Heide M Roth
    Rudolf Virchow Center for Experimental Biomedicine, Wurzburg, Germany
    DNA Repair (Amst) 11:286-93. 2012
    ..We previously characterized Bax1 from Thermoplasma acidophilum as a Mg²⁺-dependent structure-specific endonuclease...
  21. ncbi Archaeal signal peptidases from the genus Thermoplasma: structural and mechanistic hybrids of the bacterial and eukaryal enzymes
    Jerry Eichler
    J Mol Evol 54:411-5. 2002
  22. ncbi Thermoplasma acidophilum histone-like protein. Partial amino acid sequence suggestive of homology to eukaryotic histones
    D G Searcy
    Biochim Biophys Acta 609:197-200. 1980
    b>Thermoplasma acidophilum is a freeliving mycoplasma-like organism that has a small basic protein tightly bound to its DNA. The N-terminal sequence of this protein has been determined...
  23. ncbi Monomolecular organization of the main tetraether lipid from Thermoplasma acidophilum at the water-air interface
    U Bakowsky
    Institut für Physiologische Chemie der Medizinischen Fakultät, Universität Halle Wittenberg, Halle Saale, Germany
    Chem Phys Lipids 105:31-42. 2000
    The monomolecular organization of the main tetraether phospholipid from the archaeon Thermoplasma acidophilum was studied by means of a Langmuir film balance integrated into a fluorescence microscope...
  24. ncbi Purification and characterization of geranylgeranylglyceryl phosphate synthase from a thermoacidophilic archaeon, Thermoplasma acidophilum
    Naoki Nemoto
    Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    J Biochem 133:651-7. 2003
    We purified a geranylgeranylglyceryl phosphate (GGGP) synthase from Thermoplasma acidophilum by several steps of chromatography...
  25. ncbi Biosynthesis of archaeal membrane lipids: digeranylgeranylglycerophospholipid reductase of the thermoacidophilic archaeon Thermoplasma acidophilum
    Yuji Nishimura
    Department of Chemistry and Materials Science, Tokyo Institute of Technology, O Okayama, Meguro ku, Tokyo 152 8551
    J Biochem 139:1073-81. 2006
    ..reductase, was detected in a cell free extract of the thermoacidophilic archaeon Thermoplasma acidophilum...
  26. ncbi Crystal structure of the 20S proteasome from the archaeon T. acidophilum at 3.4 A resolution
    J Lowe
    Max Planck Institut fur Biochemie, Abteilung für Strukturforschung, Martinsried, Germany
    Science 268:533-9. 1995
    The three-dimensional structure of the proteasome from the archaebacterium Thermoplasma acidophilum has been elucidated by x-ray crystallographic analysis by means of isomorphous replacement and cyclic averaging...
  27. ncbi Dynamic regulation of archaeal proteasome gate opening as studied by TROSY NMR
    Tomasz L Religa
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada
    Science 328:98-102. 2010
    ..spectroscopy to show that the amino-terminal residues that compose the gates of the alpha subunits of the Thermoplasma acidophilum proteasome are highly dynamic over a broad spectrum of time scales and that gating termini are in ..
  28. pmc Mechanism of gate opening in the 20S proteasome by the proteasomal ATPases
    Julius Rabl
    Fachbereich Biologie, Chemie, Pharmazie, Freie Universitat Berlin, Takustrasse 3, 14195 Berlin, Germany
    Mol Cell 30:360-8. 2008
    ..This mechanism differs from that of PA26/28, which lacks the HbYX motif and does not cause alpha subunit rotation. These findings demonstrated how the ATPases' C termini function to facilitate substrate entry...
  29. ncbi Kinetics and mechanism of the citrate synthase from the thermophilic archaeon Thermoplasma acidophilum
    L C Kurz
    Department of Biochemistry and Molecular Biophysics, Division of Biology and Biomedical Sciences, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 39:2283-96. 2000
    The kinetics and mechanism of the citrate synthase from a moderate thermophile, Thermoplasma acidophilum (TpCS), are compared with those of the citrate synthase from a mesophile, pig heart (PCS)...
  30. doi Biochemical characterization of the minichromosome maintenance protein from the archaeon Thermoplasma acidophilum
    Gyri Teien Haugland
    Department of Biology, University of Bergen, PO Box 7800, 5020 Bergen, Norway
    Extremophiles 13:81-8. 2009
    ..Studies have shown that the MCM complex from the thermoacidophilic euryarchaeon Thermoplasma acidophilum (TaMCM) has some properties not reported in other archaeal MCM helicases...
  31. ncbi Structural analysis of the plasmid pTA1 isolated from the thermoacidophilic archaeon Thermoplasma acidophilum
    Kan Yamashiro
    Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    Extremophiles 10:327-35. 2006
    b>Thermoplasma acidophilum is a thermoacidophilic archaeon that grows optimally at pH1.8 and 56 degrees C and has no cell wall. Plasmid pTA1 was found in some strains of the species...
  32. ncbi Crystal structures of the tricorn interacting factor F3 from Thermoplasma acidophilum, a zinc aminopeptidase in three different conformations
    Otto J P Kyrieleis
    Max Planck Institut fur Biochemie, Am Klopferspitz 18, D 82152 Martinsried, Germany
    J Mol Biol 349:787-800. 2005
    The tricorn interacting factor F3 is an 89 kDa zinc aminopeptidase from the archaeon Thermoplasma acidophilum...
  33. ncbi X-ray snapshots of peptide processing in mutants of tricorn-interacting factor F1 from Thermoplasma acidophilum
    Peter Goettig
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18, D 82152 Martinsried, Germany
    J Biol Chem 280:33387-96. 2005
    The tricorn-interacting factor F1 of the archaeon Thermoplasma acidophilum cleaves small hydrophobic peptide products of the proteasome and tricorn protease...
  34. pmc Thermoplasma acidophilum Cdc6 protein stimulates MCM helicase activity by regulating its ATPase activity
    Gyri Teien Haugland
    Department of Biology, University of Bergen, N 5020 Bergen, Norway
    Nucleic Acids Res 36:5602-9. 2008
    ..To date, the only exception is the helicase and Cdc6 proteins from the archaeon Thermoplasma acidophilum...
  35. pmc Structural and mutational analysis of tRNA intron-splicing endonuclease from Thermoplasma acidophilum DSM 1728: catalytic mechanism of tRNA intron-splicing endonucleases
    Young Kwan Kim
    Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136 701, South Korea
    J Bacteriol 189:8339-46. 2007
    ..Two crystallographic structures of tRNA intron-splicing endonuclease from Thermoplasma acidophilum DSM 1728 (EndA(Ta)) have been solved to 2.5-A and 2...
  36. ncbi Quantitative proteome and transcriptome analysis of the archaeon Thermoplasma acidophilum cultured under aerobic and anaerobic conditions
    Na Sun
    Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    J Proteome Res 9:4839-50. 2010
    A comparative proteome and transcriptome analysis of Thermoplasma acidophilum cultured under aerobic and anaerobic conditions has been performed. One-thousand twenty-five proteins were identified covering 88% of the cytosolic proteome...
  37. ncbi A PPM-family protein phosphatase from the thermoacidophile Thermoplasma volcanium hydrolyzes protein-bound phosphotyrosine
    Hanan Dahche
    Department of Biochemistry, Virginia Polytechnic Institute and State University, 111C Engel Hall, Blacksburg, VA 24061, USA
    Extremophiles 13:371-7. 2009
    ..However, the distribution of their cognate protein-serine/threonine/phosphatases displays a mosaic pattern. Thermoplasma volcanium is unique among the Archaea inasmuch as it is the sole archaeon whose complement of deduced ..
  38. ncbi Purification and enzymic characterization of the cytoplasmic pyrophosphatase from the thermoacidophilic archaebacterium Thermoplasma acidophilum
    O M Richter
    Institut fur Biochemie, Medizinische Universitat zu Lubeck, Federal Republic of Germany
    Eur J Biochem 209:343-9. 1992
    Cytoplasmic pyrophosphatase has been isolated from the thermoacidophilic archaebacterium Thermoplasma acidophilum. The enzyme was purified to electrophoretic homogeneity by combining ion-exchange and affinity-chromatographic separations...
  39. doi The proteasome antechamber maintains substrates in an unfolded state
    Amy M Ruschak
    Departments of Molecular Genetics, Biochemistry and Chemistry, The University of Toronto, Toronto, Ontario M5S 1A8, Canada
    Nature 467:868-71. 2010
    ..Proteolysis occurs in a barrel-shaped 20S core particle, which in Thermoplasma acidophilum is built from four stacked homoheptameric rings of subunits, α and β, arranged α(7)β(7)β(7)α(..
  40. ncbi Discovery of the catalytic function of a putative 2-oxoacid dehydrogenase multienzyme complex in the thermophilic archaeon Thermoplasma acidophilum
    Caroline Heath
    Centre for Extremophile Research, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    FEBS Lett 577:523-7. 2004
    ..current paper, we have cloned and expressed the first two genes of this operon from the thermophilic archaeon, Thermoplasma acidophilum...
  41. pmc Stimulation of MCM helicase activity by a Cdc6 protein in the archaeon Thermoplasma acidophilum
    Gyri Teien Haugland
    Department of Biology, University of Bergen, PO Box 7800, N 5020 Bergen, Norway
    Nucleic Acids Res 34:6337-44. 2006
    ..In this report the biochemical properties of the MCM protein from the archaeon Thermoplasma acidophilum is described...
  42. pmc Archaeal-eubacterial mergers in the origin of Eukarya: phylogenetic classification of life
    L Margulis
    Department of Biology, University of Massachusetts, Amherst 01003 5810, USA
    Proc Natl Acad Sci U S A 93:1071-6. 1996
    ..g., Thermoplasma-like organisms) and of Eubacteria (e.g., Spirochaeta-like organisms)...
  43. ncbi Characterization of the AP endonucleases from Thermoplasma volcanium and Lactobacillus plantarum: Contributions of two important tryptophan residues to AP site recognition
    Kohichi Kaneda
    Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan
    Biosci Biotechnol Biochem 70:2213-21. 2006
    ..To elucidate whether these residues are involved in AP site recognition, the ExoIII homologs of Thermoplasma volcanium and Lactobacillus plantarum were characterized...
  44. pmc An actin homolog of the archaeon Thermoplasma acidophilum that retains the ancient characteristics of eukaryotic actin
    Futoshi Hara
    Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192 0392, Japan
    J Bacteriol 189:2039-45. 2007
    ..Recently, the structure of the archaeal actin homolog Ta0583, isolated from the archaeon Thermoplasma acidophilum, which lacks a cell wall, was reported by Roeben et al. (J. Mol. Biol. 358:145-156, 2006)...
  45. pmc The intein of the Thermoplasma A-ATPase A subunit: structure, evolution and expression in E. coli
    A G Senejani
    Department of Molecular and Cell Biology, University of Connecticut, 75 North Eagleville Rd Storrs, CT 06269 3044, USA
    BMC Biochem 2:13. 2001
    ..In addition to this splicing activity, most reported inteins also contain an endonuclease domain that is important in intein propagation...
  46. ncbi Proteomics analysis of Thermoplasma acidophilum with a focus on protein complexes
    Na Sun
    Department of Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried bei munchen, Germany
    Mol Cell Proteomics 6:492-502. 2007
    ..2DE) and MALDI-TOF MS were used to obtain a global view of the cytoplasmic proteins expressed by Thermoplasma acidophilum...
  47. ncbi Proteasome function is dispensable under normal but not under heat shock conditions in Thermoplasma acidophilum
    A Ruepp
    Max Planck Institut fur Biochemie, Martinsried, Germany
    FEBS Lett 425:87-90. 1998
    ..carboxybenzyl-leucyl-leucyl-leucine vinyl sulfone (Z-L3VS) to study the in vivo function of proteasomes in Thermoplasma acidophilum...
  48. pmc Isolation and characterization of flagella and flagellin proteins from the Thermoacidophilic archaea Thermoplasma volcanium and Sulfolobus shibatae
    D M Faguy
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Bacteriol 178:902-5. 1996
    ..The flagellar filaments of Thermoplasma volcanium were 12 nm in diameter and were composed of one major flagellin which has an M(r) of 41,000 and which ..
  49. ncbi Photophysics of tryptophan fluorescence: link with the catalytic strategy of the citrate synthase from Thermoplasma acidophilum
    Linda C Kurz
    Department of Biochemistry and Molecular Biophysics, Division of Biology and Biomedical Sciences, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 44:1394-413. 2005
    The formation of all major intermediates in the reaction catalyzed by the citrate synthase from Thermoplasma acidophilum is accompanied by changes in tryptophan fluorescence...
  50. ncbi Mechanosensitive channel of Thermoplasma, the cell wall-less archaea: cloning and molecular characterization
    A Kloda
    Department of Pharmacology, QEII Medical Center, The University of Western Australia, Nedlands, Australia
    Cell Biochem Biophys 34:321-47. 2001
    ..experiments, we identified a novel mechanosensitive (MS) channel in the thermophilic cell wall-less archaeon Thermoplasma volcanium...
  51. ncbi Mutational analysis of conserved AAA+ residues in the archaeal Lon protease from Thermoplasma acidophilum
    Henrike Besche
    Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, 82152 Martinsried, Germany
    FEBS Lett 574:161-6. 2004
    The Lon protease from the archaeon Thermoplasma acidophilum (TaLon) is composed of an N-terminal ATPase associated with various cellular activities (AAA+) domain and a C-terminal Lon protease domain...
  52. pmc Effects of a squalene epoxidase inhibitor, terbinafine, on ether lipid biosyntheses in a thermoacidophilic archaeon, Thermoplasma acidophilum
    Takahide Kon
    Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Tokyo 153 8902
    J Bacteriol 184:1395-401. 2002
    ..epoxidase inhibitor terbinafine on the growth and ether lipid biosyntheses in the thermoacidophilic archaeon Thermoplasma acidophilum. Terbinafine was found to inhibit the growth of T. acidophilum in a concentration-dependent manner...
  53. pmc Structure- and function-based characterization of a new phosphoglycolate phosphatase from Thermoplasma acidophilum
    Youngchang Kim
    Argonne National Laboratory, Structural Biology Center, Argonne, Illinois 60439, USA
    J Biol Chem 279:517-26. 2004
    ..structure information, and kinetic analysis, we have characterized TA0175 as phosphoglycolate phosphatase from Thermoplasma acidophilum...
  54. ncbi Structure of a putative lipoate protein ligase from Thermoplasma acidophilum and the mechanism of target selection for post-translational modification
    Edward McManus
    Department of Biochemistry, University of Cambridge, Old Addenbrooke s Site, Sanger Building, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    J Mol Biol 356:625-37. 2006
    ..A gene encoding a putative lipoate protein ligase (LplA) of Thermoplasma acidophilum was cloned and expressed in Escherichia coli...
  55. ncbi Crystal structure of a nicotinate phosphoribosyltransferase from Thermoplasma acidophilum
    Dong Hae Shin
    Berkeley Structural Genomics Center, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    J Biol Chem 280:18326-35. 2005
    ..The structures of TaNAPRTase also provide valuable insight for other sequence subfamilies such as pre-B cell colony-enhancing factor, known to have nicotinamide phosphoribosyltransferase activity...
  56. ncbi Primary structure of the Thermoplasma proteasome and its implications for the structure, function, and evolution of the multicatalytic proteinase
    P Zwickl
    Max Planck Institut fur Biochemie, Martinsried, FRG
    Biochemistry 31:964-72. 1992
    ..On the basis of circumstantial evidence we suggest that the alpha-subunits have regulatory and targeting functions, while the beta-subunits carry the active sites...
  57. ncbi The Thermoplasma acidophilum Lon protease has a Ser-Lys dyad active site
    Henrike Besche
    Department of Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Eur J Biochem 271:4361-5. 2004
    ..to bacterial Lon proteases was identified during the sequencing of the genome of the thermoacidophilic archaeon Thermoplasma acidophilum...
  58. ncbi Structural insights into unique substrate selectivity of Thermoplasma acidophilum D-aldohexose dehydrogenase
    Yoshiaki Yasutake
    Research Institute of Genome Based Biofactory, National Institute of Advanced Industrial Science and Technology, Toyohira ku, Sapporo 062 8517, Japan
    J Mol Biol 367:1034-46. 2007
    The D-aldohexose dehydrogenase from the thermoacidophilic archaea Thermoplasma acidophilum (AldT) belongs to the short-chain dehydrogenase/reductase (SDR) superfamily and catalyzes the oxidation of several monosaccharides with a ..
  59. ncbi Crystal structure of an archaeal actin homolog
    Annette Roeben
    Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Mol Biol 358:145-56. 2006
    ..As a first step, we expressed a predicted actin homolog of the thermophilic archaeon Thermoplasma acidophilum, Ta0583, and determined its crystal structure at 2.1A resolution...
  60. ncbi Resonance assignments for the hypothetical protein TA0938 from Thermoplasma acidophilum
    Daniel Monleon
    Department of Physical Chemistry, University of Valencia, C Dr Moliner 50, Burjassot, 46100, Valencia, Spain
    J Biomol NMR 36:36. 2006
  61. pmc Solution structure of the hypothetical protein TA0095 from Thermoplasma acidophilum: a novel superfamily with a two-layer sandwich architecture
    Esther León
    Instituto de Quimica Fisica Rocasolano, Madrid, Spain
    Protein Sci 16:2278-86. 2007
    TA0095 is a 96-residue hypothetical protein from Thermoplasma acidophilum that exhibits no sequence similarity to any protein of known structure...
  62. pmc Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative transposase from Thermoplasma acidophilum encoded by the Ta0474 gene
    Ji Yong Kang
    Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151 742, South Korea
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:1147-9. 2006
    ..among IS200-like transposases, structural analysis has been initiated of a putative transposase from Thermoplasma acidophilum encoded by the Ta0474 gene...
  63. ncbi Solution structure of TA0895, a MoaD homologue from Thermoplasma acidophilum
    Jinwon Jung
    Department of Biochemistry, College of Science and National Research Laboratory, Yonsei University, Seoul 120 749, Korea
    Proteins 65:1055-7. 2006
  64. pmc The pore of activated 20S proteasomes has an ordered 7-fold symmetric conformation
    Andreas Forster
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    EMBO J 22:4356-64. 2003
    ..Evolutionary considerations suggest that other activators might induce the same open proteasome conformation as seen with the 11S activator...
  65. ncbi Proteasome-cytochrome c interactions: a model system for investigation of proteasome host-guest interactions
    Holly A Huffman
    Department of Biochemistry, University of California, Riverside, California 92521, USA
    Biochemistry 42:8679-86. 2003
    Owing to its high thermal stability and structural simplicity, the archaebacterium Thermoplasma Acidophilum 20S proteasome was selected for mechanistic studies in this work...
  66. ncbi The asymmetric ATPase cycle of the thermosome: elucidation of the binding, hydrolysis and product-release steps
    Maria Giulia Bigotti
    Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK
    J Mol Biol 362:835-43. 2006
    ..we have begun to dissect the process of energy transduction in the thermosome, a type II chaperonin from Thermoplasma acidophilum...
  67. pmc Crystal structure of the hypothetical protein TA1238 from Thermoplasma acidophilum: a new type of helical super-bundle
    Ruslan Sanishvili
    Biosciences, Argonne National Laboratory, Structural Biology Center, Midwest Center for Structural Genomics, 9700 South Cass Avenue, Argonne, IL 60439, USA
    J Struct Funct Genomics 5:231-40. 2004
    The crystal structure of the hypothetical protein TA1238 from Thermoplasma acidophilum was solved with multiple-wavelength anomalous diffraction and refined at 2.0 A resolution...
  68. ncbi Interaction analysis of the AAA ATPase TAA43 by the bacterial two-hybrid system
    Leticia Santos
    Division of Molecular Biology, Institute for Scientific and Technological Research of San Luis Potosi, San Luis Potosi, Mexico
    FEMS Microbiol Lett 254:293-9. 2006
    The TAA43 ATPase of Thermoplasma acidophilum, an archaeal member of the AAA protein family, is known to have an atypical oligomeric state and a nonspecific association with high-molecular-weight protein complexes...
  69. ncbi NMR structure of the conserved novel-fold protein TA0743 from Thermoplasma acidophilum
    Beomkyu Kim
    Department of Biochemistry and HTSD NMR Application NRL, Yonsei University, Seoul, Korea
    Proteins 62:819-21. 2006
  70. ncbi NMR structure of hypothetical protein TA0938 from Thermoplasma acidophilum
    Daniel Monleon
    Department of Physical Chemistry, University of Valencia, Burjassot 46100, Valencia, Spain
    Proteins 67:1185-8. 2007
  71. ncbi Stereochemistry of reduction in digeranylgeranylglycerophospholipid reductase involved in the biosynthesis of archaeal membrane lipids from Thermoplasma acidophilum
    Yuji Nishimura
    Department of Chemistry and Materials Science, Tokyo Institute of Technology, O Okayama, Meguro ku, Tokyo 152 8551, Japan
    Bioorg Chem 35:276-83. 2007
    ..The putative DGGGPL reductase gene (Ta0516m) of Thermoplasma acidophilum was cloned and expressed...
  72. ncbi Crystal structure of bovine lipoyltransferase in complex with lipoyl-AMP
    Kazuko Fujiwara
    Institute for Enzyme Research, The University of Tokushima, Tokushima 770 8503, Japan
    J Mol Biol 371:222-34. 2007
    ..We discuss the structural differences between bLT and the lipoate-protein ligase A from Escherichia coli and Thermoplasma acidophilum...
  73. ncbi The 2-oxoacid dehydrogenase multienzyme complex of Haloferax volcanii
    Dina M Al-Mailem
    Department of Biological Sciences, Faculty of Science, Kuwait University, PO Box 5969, Safat 13060, State of Kuwait
    Extremophiles 12:89-96. 2008
    ..However, no catalytic activity of any such complex has ever been detected in the archaea. In Thermoplasma acidophilum, evidence has been presented that the heterologously expressed recombinant enzyme possesses ..
  74. ncbi Solution structure of hypothetical protein TA1414 from Thermoplasma acidophilum
    Daniel Monleon
    J Biomol NMR 28:81-4. 2004
  75. ncbi Purification and analysis of recombinant 11S activators of the 20S proteasome: Trypanosoma brucei PA26 and human PA28 alpha, PA28 beta, and PA28 gamma
    Eugene I Masters
    Department of Biology, University of Utah, Salt Lake City, UT 84103, USA
    Methods Enzymol 398:306-21. 2005
    ..This chapter describes protocols for the purification of recombinant 11S regulators, characterization of their ability to stimulate proteasome activity, and crystallization of proteasome complexes...
  76. ncbi Cultivated anaerobic acidophilic/acidotolerant thermophiles from terrestrial and deep-sea hydrothermal habitats
    Maria I Prokofeva
    Institute of Microbiology, Russian Academy of Sciences, Prospect 60 Letya Oktyabrya 7 2, 117312 Moscow, Russia
    Extremophiles 9:437-48. 2005
    ..obtained from terrestrial enrichment cultures growing at 60 degrees C revealed the presence of archaea of genus Thermoplasma and bacteria of genus Thermoanaerobacter...
  77. pmc Biochemical and structural characterization of a novel family of cystathionine beta-synthase domain proteins fused to a Zn ribbon-like domain
    Michael Proudfoot
    Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Room 72, Toronto, ON, Canada
    J Mol Biol 375:301-15. 2008
    ..Four proteins were overexpressed in Escherichia coli and purified: TA0289 from Thermoplasma acidophilum, TV1335 from Thermoplasma volcanium, PF1953 from Pyrococcus furiosus, and PH0267 from Pyrococcus ..
  78. ncbi Tricorn protease--the core of a modular proteolytic system
    T Tamura
    Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Science 274:1385-9. 1996
    ..A tricorn-shaped protease was isolated from the archaeon Thermoplasma and was shown to form a multisubunit proteolytic complex...
  79. ncbi Cloning and characterization of ftsZ and pyrF from the archaeon Thermoplasma acidophilum
    T Yaoi
    NASA Ames Research Center, Moffett Field, California 94035, USA
    Biochem Biophys Res Commun 275:936-45. 2000
    To characterize cytoskeletal components of archaea, the ftsZ gene from Thermoplasma acidophilum was cloned and sequenced. In T...
  80. ncbi Crystal structure of the beta-apical domain of the thermosome reveals structural plasticity in the protrusion region
    G Bosch
    Max Planck Institute for Biochemistry, Am Klopferspitz 18a, Martinsried bei Munchen, D 82152, Germany
    J Mol Biol 301:19-25. 2000
    The crystal structure of the beta-apical domain of the thermosome, an archaeal group II chaperonin from Thermoplasma acidophilum, has been determined at 2.8 A resolution...
  81. ncbi Novel zinc-containing ferredoxin family in thermoacidophilic archaea
    T Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113, Japan
    J Biol Chem 272:3453-8. 1997
    The dicluster-type ferredoxins from the thermoacidophilic archaea such as Thermoplasma acidophilum and Sulfolobus sp. are known to contain an unusually long extension of unknown function in the N-terminal region...
  82. ncbi Characterization of cofactor-dependent and cofactor-independent phosphoglycerate mutases from Archaea
    Ulrike Johnsen
    Institut fur Allgemeine Mikrobiologie, Christian Albrechts Universitat Kiel, Am Botanischen Garten 1 9, 24118, Kiel, Germany
    Extremophiles 11:647-57. 2007
    ..Here, we report the characterization of the first archaeal cofactor-dependent PGM from Thermoplasma acidophilum, which is encoded by ORF TA1347...
  83. ncbi Gene duplication and the evolution of group II chaperonins: implications for structure and function
    J M Archibald
    Program in Evolutionary Biology, Canadian Institute for Advanced Research, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada
    J Struct Biol 135:157-69. 2001
    ..When mapped onto the crystal structure of the archaeal chaperonin from Thermoplasma acidophilum, these signatures are located in the apical, intermediate, and equatorial domains...
  84. pmc Crystal structure of heat shock locus V (HslV) from Escherichia coli
    M Bochtler
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Proc Natl Acad Sci U S A 94:6070-4. 1997
    ..A tripeptide aldehyde inhibitor, acetyl-Leu-Leu-norleucinal, binds to the N-terminal threonine residue of HslV, probably as a hemiacetal, relating HslV also functionally to the proteasomes of archaea and eukaryotes...
  85. ncbi Electron cryo-microscopy of VAT, the archaeal p97/CDC48 homologue from Thermoplasma acidophilum
    Beate Rockel
    Max Planck Institut fur Biochemie, Abteilung Molekulare Strukturbiologie, Am Klopferspitz 18 A, 82152 Martinsried, Germany
    J Mol Biol 317:673-81. 2002
    VAT (valosine containing protein-like ATPase from Thermoplasma acidophilum), an archaeal member of the AAA-family (ATPases associated with a variety of cellular activities) that possesses foldase as well as unfoldase-activity, forms homo-..
  86. pmc Structural insights into an equilibrium folding intermediate of an archaeal ankyrin repeat protein
    Christian Löw
    Institut fur Physik, Biophysik, Martin Luther Universitat Halle Wittenberg, D 06120 Halle Saale, Germany
    Proc Natl Acad Sci U S A 105:3779-84. 2008
    ..we studied the structure and folding of a new thermophilic AR protein (tANK) identified in the archaeon Thermoplasma volcanium...
  87. ncbi Structural and functional insights into Dom34, a key component of no-go mRNA decay
    Hyung Ho Lee
    Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151 747, Korea
    Mol Cell 27:938-50. 2007
    ..gain insights into the structure and function of Dom34/Pelota, we have determined the structure of Pelota from Thermoplasma acidophilum (Ta Pelota) and investigated the ribonuclease activity of Dom34/Pelota...
  88. pmc Crystal structure of a predicted precorrin-8x methylmutase from Thermoplasma acidophilum
    M E Cuff
    Biosciences Division, Structural Biology Center, Argonne National Laboratory, Argonne, Illinois 60439, USA
    Proteins 58:751-4. 2005
  89. ncbi Tricorn protease exists as an icosahedral supermolecule in vivo
    J Walz
    Abteilung Molekulare Strukturbiologie, Max Planck Institut fur Biochemie, Martinsried, Germany
    Mol Cell 1:59-65. 1997
    ..We suggest that the tricorn capsid, in addition to its intrinsic proteolytic activity, serves as the organizing center of a multienzyme complex...
  90. ncbi 3D reconstruction of the ATP-bound form of CCT reveals the asymmetric folding conformation of a type II chaperonin
    O Llorca
    Centro Nacional de Biotecnologia, CSIC, Campus Universidad Autonoma de Madrid, Spain
    Nat Struct Biol 6:639-42. 1999
    ..Upon ATP binding the apical domains rotate and point towards the cylinder axis, so that the helical protrusions present at their tips could act as a lid closing the ring cavity...
  91. ncbi Capsids of tricorn protease studied by electron cryomicroscopy
    J Walz
    Department of Molecular Structural Biology, Max Planck Institut fur Biochemie, Martinsried, 82152, Germany
    J Struct Biol 128:65-8. 1999
    Tricorn protease from the archaeon Thermoplasma acidophilum acts "downstream" of the proteasome; in conjunction with its aminopeptidase cofactors it converts peptides generated by the proteasome into free amino acids...
  92. pmc Phylogenomic analysis of proteins that are distinctive of Archaea and its main subgroups and the origin of methanogenesis
    Beile Gao
    Department of Biochemistry and Biomedical Science, McMaster University, Hamilton, Canada
    BMC Genomics 8:86. 2007
    ..The evolutionary relationships among different groups within the Euryarchaeota branch are also not clearly understood...
  93. ncbi Expression of long- and short-type FK506 binding proteins in hyperthermophilic archaea
    Akira Ideno
    Marine Biotechnology Institute, 3 75 1 Heita, Kamaishi, Iwate 026 0001, Japan
    Gene 292:57-63. 2002
    ..FKBPs showed chaperone-like protein-folding activity to enhance the refolding yield of an unfolded protein (Thermoplasma citrate synthase) in vitro...
  94. ncbi Early evolutionary relationships among known life forms inferred from elongation factor EF-2/EF-G sequences: phylogenetic coherence and structure of the archaeal domain
    P Cammarano
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Biopatologia Umana, Universita di Roma, La Sapienza, Roma, Italy
    J Mol Evol 34:396-405. 1992
    ..deepest bifurcation divides a Sulfolobus branch from a branch comprising Methanococcus, Halobacterium, and Thermoplasma. Bootstrapped distance-matrix treeing confirmed the monophyly-holophyly of Archaea in 100% of the samples and ..
  95. pmc ADPase activity of recombinantly expressed thermotolerant ATPases may be caused by copurification of adenylate kinase of Escherichia coli
    Baoyu Chen
    Integrative Biosciences Graduate Degree Program Chemical Biology, The Pennsylvania State University, University Park, PA, USA
    FEBS J 276:807-15. 2009
    ..of the ATPase chaperonins from Pyrococcus furiosus, Pyrococcus horikoshii, Methanococcus jannaschii and Thermoplasma acidophilum; the ATP/ADP-dependent DNA ligases from Aeropyrum pernix K1 and Staphylothermus marinus; or the ..
  96. ncbi Bacterial origin for the isoprenoid biosynthesis enzyme HMG-CoA reductase of the archaeal orders Thermoplasmatales and Archaeoglobales
    Y Boucher
    Canadian Institute for Advanced Research, Dalhousie University, Halifax, Nova Scotia, Canada
    Mol Biol Evol 18:1378-88. 2001
    ..Surprisingly, two species of the genus Thermoplasma also harbor an HMGR of bacterial origin highly similar to the enzymes found in the Archaeoglobales...
  97. ncbi Characteristic archaebacterial 16S rRNA oligonucleotides
    T J McGill
    Department of Biochemical Sciences and Biophysical Sciences, University of Houston University Park, Texas 77004, USA
    Syst Appl Microbiol 7:194-7. 1986
    ..The signature approach also was used to assess the phylogenetic position of Thermoplasma acidophilum which is found to be more closely related to the methanogen/halophile Division than to the sulfur ..
  98. pmc Molecular microbial diversity of an anaerobic digestor as determined by small-subunit rDNA sequence analysis
    J J Godon
    Laboratoire de Biotechnologie de l Environnement, Institut National de la Recherche Agronomique, Narbonne, France
    Appl Environ Microbiol 63:2802-13. 1997
    ..In contrast, the three other Archaea OTUs are unusual and are related to thermophilic microorganisms such as Crenarchaea or Thermoplasma spp. Five percent of the sequences analyzed were chimeras and were removed from the analysis.
  99. pmc B12-dependent ribonucleotide reductases from deeply rooted eubacteria are structurally related to the aerobic enzyme from Escherichia coli
    A Jordan
    Department of Biochemistry I, Medical Nobel Institute, MBB, Karolinska Institute, S 17177 Stockholm, Sweden
    Proc Natl Acad Sci U S A 94:13487-92. 1997
    ..to be coenzyme-B12 (class II) enzymes, similar to the earlier described reductases from the archaebacteria Thermoplasma acidophila and Pyrococcus furiosus...
  100. pmc Identification of and spatio-temporal differences between microbial assemblages from two neighboring sulfurous lakes: comparison by microscopy and denaturing gradient gel electrophoresis
    E O Casamayor
    Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar CSIC, E 08039 Barcelona, Spain
    Appl Environ Microbiol 66:499-508. 2000
    ..Euryarchaeal sequences (i.e., methanogen- and thermoplasma-related sequences) also were present in both lakes...
  101. ncbi Archaeal genomics
    T Gaasterland
    Laboratory of Computational Genomics 1230 York Avenue The Rockefeller University New York, New York, 10021, USA
    Curr Opin Microbiol 2:542-7. 1999
    ..In addition, seven more archaeal genome sequencing projects are under way, including two halophiles, two Thermoplasma, and a methanogen...

Research Grants13

  1. Mechanisms of Regulation of the Proteosome Activities
    Maria Gaczynska; Fiscal Year: 2007
    ..The proposed studies will provide a basis for the future targeting of the proteasomal activities with noncompetitive modulators interacting with the a rings and ultimately 19S caps. [unreadable] [unreadable]..
  2. Mechanisms of Regulation of the Proteosome Activities
    Maria Gaczynska; Fiscal Year: 2005
    ..The proposed studies will provide a basis for the future targeting of the proteasomal activities with noncompetitive modulators interacting with the a rings and ultimately 19S caps. ..
  3. Mechanisms of Regulation of the Proteosome Activities
    Maria Gaczynska; Fiscal Year: 2006
    ..The proposed studies will provide a basis for the future targeting of the proteasomal activities with noncompetitive modulators interacting with the a rings and ultimately 19S caps. [unreadable] [unreadable]..
  4. Mechanisms of Regulation of the Proteosome Activities
    Maria Gaczynska; Fiscal Year: 2005
    ..The proposed studies will provide a basis for the future targeting of the proteasomal activities with noncompetitive modulators interacting with the a rings and ultimately 19S caps. ..
  5. Mechanisms of Regulation of the Proteasome Activities
    Maria Gaczynska; Fiscal Year: 2004
    ..The proposed studies will provide a basis for the future targeting of the proteasomal activities with noncompetitive modulators interacting with the a rings and ultimately 19S caps. ..
  6. MECHANISMS OF ENZYMES WHICH FORM CARBON CARBON BONDS
    Linda Kurz; Fiscal Year: 2004
    ..We will test predictions based on previous experimental and theoretical work. ..
  7. MECHANISMS OF ENZYMES WHICH FORM CARBON CARBON BONDS
    Linda Kurz; Fiscal Year: 2003
    ..We will test predictions based on previous experimental and theoretical work. ..
  8. Conference--The Global Genome: Microbes
    EDWARD DELONG; Fiscal Year: 2002
    ..The colloquium will be convened to develop the intellectual material that will comprise an analytical and comprehensive report. ..
  9. Aging /Caloric Restriction /Cytosolic Protease
    Maria Gaczynska; Fiscal Year: 2002
    ..These studies will provide us with detailed pattern of age- dependent and caloric restriction-dependent changes in cellular nonlysosomal controlled proteolysis. ..
  10. MECHANISMS OF ENZYMES WHICH FORM CARBON CARBON BONDS
    Linda Kurz; Fiscal Year: 2002
    ..We will test predictions based on previous experimental and theoretical work. ..
  11. MECHANISMS OF ENZYMES WHICH FORM CARBON CARBON BONDS
    Linda Kurz; Fiscal Year: 2001
    ..We will test predictions based on previous experimental and theoretical work. ..
  12. Mechanisms of Regulation of the Proteosome Activities
    Maria Gaczynska; Fiscal Year: 2008
    ..The proposed studies will provide a basis for the future targeting of the proteasomal activities with noncompetitive modulators interacting with the a rings and ultimately 19S caps. [unreadable] [unreadable]..