methanococcus

Summary

Summary: A genus of anaerobic coccoid METHANOCOCCACEAE whose organisms are motile by means of polar tufts of flagella. These methanogens are found in salt marshes, marine and estuarine sediments, and the intestinal tract of animals.

Top Publications

  1. pmc Characterization of CRISPR RNA processing in Clostridium thermocellum and Methanococcus maripaludis
    Hagen Richter
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse 10, D 35037 Marburg, Germany
    Nucleic Acids Res 40:9887-96. 2012
  2. pmc Metabolic modeling of a mutualistic microbial community
    Sergey Stolyar
    Department of Civil and Environmental Engineering, University of Washington, Seattle, WA 98195, USA
    Mol Syst Biol 3:92. 2007
  3. ncbi Methanococcus aeolicus sp. nov., a mesophilic, methanogenic archaeon from shallow and deep marine sediments
    Melissa M Kendall
    Biology Department, Portland State University, PO Box 751, Portland, OR 97207 0751, USA
    Int J Syst Evol Microbiol 56:1525-9. 2006
  4. doi Flagella and pili are both necessary for efficient attachment of Methanococcus maripaludis to surfaces
    Ken F Jarrell
    Department of Microbiology and Immunology, Queen s University, Kingston, ON, Canada
    FEMS Microbiol Lett 319:44-50. 2011
  5. ncbi RNA methylation under heat shock control
    H Bugl
    Department of Biology, University of Michigan, Ann Arbor 48109, USA
    Mol Cell 6:349-60. 2000
  6. pmc Metabolic network model of a human oral pathogen
    Varun Mazumdar
    Boston University, Bioinformatics Program, Boston, MA 02215, USA
    J Bacteriol 191:74-90. 2009
  7. ncbi X-ray structure of a protein-conducting channel
    Bert van den Berg
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:36-44. 2004
  8. pmc Characterization of guanine and hypoxanthine phosphoribosyltransferases in Methanococcus voltae
    T L Bowen
    Department of Microbiology, University of Georgia, Athens 30602 2605, USA
    J Bacteriol 178:2521-6. 1996
  9. pmc Identification and characterization of a DNA primase from the hyperthermophilic archaeon Methanococcus jannaschii
    G Desogus
    Imperial College of Science, Technology, and Medicine, Biophysics Section, Blackett Laboratory, London SW7 2BZ, UK
    Nucleic Acids Res 27:4444-50. 1999
  10. pmc pH-induced structural change in a sodium/proton antiporter from Methanococcus jannaschii
    Kutti R Vinothkumar
    Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany
    EMBO J 24:2720-9. 2005

Research Grants

  1. Archaeal gene recruitment by the Chlamydiales
    David Graham; Fiscal Year: 2007
  2. Aminoacyl-tRNA synthetases in Archaea
    Ya Ming Hou; Fiscal Year: 2003
  3. SERYL-TRNA SYNTHETASES: EVOLUTION AND DIVERSITY
    Dieter Soll; Fiscal Year: 2001
  4. SERYL-TRNA SYNTHETASES: EVOLUTION AND DIVERSITY
    Dieter Soll; Fiscal Year: 2002
  5. SERYL-TRNA SYNTHETASES: EVOLUTION AND DIVERSITY
    Dieter Soll; Fiscal Year: 2003
  6. Membrane Protein Folding and Assembly
    Stephen H White; Fiscal Year: 2011
  7. FEN1 STRUCTURES AND HUMAN GENOME STABILITY
    John Tainer; Fiscal Year: 2000
  8. FEN1 STRUCTURES AND HUMAN GENOME STABILITY
    John Tainer; Fiscal Year: 1999
  9. FEN1 STRUCTURES AND HUMAN GENOME STABILITY
    John Tainer; Fiscal Year: 2002
  10. FEN1 STRUCTURES AND HUMAN GENOME STABILITY
    John Tainer; Fiscal Year: 2001

Detail Information

Publications244 found, 100 shown here

  1. pmc Characterization of CRISPR RNA processing in Clostridium thermocellum and Methanococcus maripaludis
    Hagen Richter
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse 10, D 35037 Marburg, Germany
    Nucleic Acids Res 40:9887-96. 2012
    ..of novel Cas6 enzymes from the bacterial and archaeal model organisms Clostridium thermocellum and Methanococcus maripaludis C5. Methanococcus maripaludis Cas6b in vitro activity and specificity was determined...
  2. pmc Metabolic modeling of a mutualistic microbial community
    Sergey Stolyar
    Department of Civil and Environmental Engineering, University of Washington, Seattle, WA 98195, USA
    Mol Syst Biol 3:92. 2007
    ..of these relationships, we took advantage of the fully sequenced genomes of Desulfovibrio vulgaris and Methanococcus maripaludis to produce and analyze the first multispecies stoichiometric metabolic model...
  3. ncbi Methanococcus aeolicus sp. nov., a mesophilic, methanogenic archaeon from shallow and deep marine sediments
    Melissa M Kendall
    Biology Department, Portland State University, PO Box 751, Portland, OR 97207 0751, USA
    Int J Syst Evol Microbiol 56:1525-9. 2006
    ..and Nankai-3(T) grew at temperatures that were more similar to those of recognized species within the genus Methanococcus. Strain Nankai-3(T) grew fastest at 46 degrees C...
  4. doi Flagella and pili are both necessary for efficient attachment of Methanococcus maripaludis to surfaces
    Ken F Jarrell
    Department of Microbiology and Immunology, Queen s University, Kingston, ON, Canada
    FEMS Microbiol Lett 319:44-50. 2011
    b>Methanococcus maripaludis has two surface appendages, namely flagella and pili. Flagella have been shown to be required for swimming, but no specific role has been assigned as yet to pili. In this report, wild-type M...
  5. ncbi RNA methylation under heat shock control
    H Bugl
    Department of Biology, University of Michigan, Ann Arbor 48109, USA
    Mol Cell 6:349-60. 2000
    ..Null mutations in ftsJ show a dramatically altered ribosome profile, a severe growth disadvantage, and a temperature-sensitive phenotype. Our results reveal an unexpected link between the heat shock response and RNA metabolism...
  6. pmc Metabolic network model of a human oral pathogen
    Varun Mazumdar
    Boston University, Bioinformatics Program, Boston, MA 02215, USA
    J Bacteriol 191:74-90. 2009
    ..The current model, which is amenable to further experimental testing and refinements, could prove useful in evaluating the oral microbiome dynamics and in the development of novel biomedical applications...
  7. ncbi X-ray structure of a protein-conducting channel
    Bert van den Berg
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:36-44. 2004
    ..We report the crystal structure of the complex from Methanococcus jannaschii at a resolution of 3.2 A...
  8. pmc Characterization of guanine and hypoxanthine phosphoribosyltransferases in Methanococcus voltae
    T L Bowen
    Department of Microbiology, University of Georgia, Athens 30602 2605, USA
    J Bacteriol 178:2521-6. 1996
    ..PRTase) and nucleoside phosphorylase (NPase) activities were detected by radiometric methods in extracts of Methanococcus voltae. Guanine PRTase activity was present at 2...
  9. pmc Identification and characterization of a DNA primase from the hyperthermophilic archaeon Methanococcus jannaschii
    G Desogus
    Imperial College of Science, Technology, and Medicine, Biophysics Section, Blackett Laboratory, London SW7 2BZ, UK
    Nucleic Acids Res 27:4444-50. 1999
    We report the identification and characterisation of a DNA primase from the thermophilic methanogenic archaeon Methanococcus jannaschii (Mjpri)...
  10. pmc pH-induced structural change in a sodium/proton antiporter from Methanococcus jannaschii
    Kutti R Vinothkumar
    Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany
    EMBO J 24:2720-9. 2005
    ..MjNhaP1 from Methanococcus jannaschii, a hyperthermophilic archaeon that grows optimally at 85 degrees C, was cloned and expressed in ..
  11. ncbi Calcium stiffens archaeal Rad51 recombinase from Methanococcus voltae for homologous recombination
    Xinguo Qian
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
    J Biol Chem 281:39380-7. 2006
    ..that the strand exchange activity but not the ATPase activity of an archaeal RadA/Rad51 recombinase from Methanococcus voltae (MvRadA) is also subject to calcium stimulation...
  12. ncbi Catalytic and thermodynamic properties of tetrahydromethanopterin-dependent serine hydroxymethyltransferase from Methanococcus jannaschii
    Sebastiana Angelaccio
    Dipartimento di Scienze Biochimiche A Rossi Fanelli, Universita degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, Rome 00185, Italy
    J Biol Chem 278:41789-97. 2003
    ..reports on the purification and characterization of recombinant SHMT from the hyperthermophilic methanogen Methanococcus jannaschii...
  13. pmc Rapid evolution of stability and productivity at the origin of a microbial mutualism
    Kristina L Hillesland
    Department of Civil and Environmental Engineering, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 107:2124-9. 2010
    ..Twenty-four independent pairings (cocultures) of the bacterium Desulfovibrio vulgaris and the archaeon Methanococcus maripaludis were established and followed for 300 community doublings in two environments, one allowing for ..
  14. ncbi Translation termination factor aRF1 from the archaeon Methanococcus jannaschii is active with eukaryotic ribosomes
    M Dontsova
    Institute of Protein Research, Russian Academy of Sciences, 142292, Pushchino, Russia
    FEBS Lett 472:213-6. 2000
    ..expression in Escherichia coli of the gene encoding the peptide chain RF from the hyperthermophilic archaeon Methanococcus jannaschii (MjaRF1). In an in vitro assay with mammalian ribosomes, MjaRF1, which was overproduced in E...
  15. pmc Crystal structure of a DNA binding protein from the hyperthermophilic euryarchaeon Methanococcus jannaschii
    Ganggang Wang
    MOE Laboratory of Protein Science and Laboratory of Structural Biology, Department of Biological Science and Biotechnology, Tsinghua University, Beijing, 100084, People s Republic of China
    Protein Sci 12:2815-22. 2003
    ..Here, we report the 2.0 A resolution crystal structure of Mja10b from the euryarchaeon Methanococcus jannaschii. The model of Mja10b has been refined to an R-factor of 20.9%...
  16. pmc AglC and AglK are involved in biosynthesis and attachment of diacetylated glucuronic acid to the N-glycan in Methanococcus voltae
    Bonnie Chaban
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, K7L 3N6, Canada
    J Bacteriol 191:187-95. 2009
    ..To continue the study of the Methanococcus voltae N-glycosylation pathway, characteristics of known eukaryotic, bacterial, and archaeal proteins involved ..
  17. pmc Genetic and mass spectrometry analyses of the unusual type IV-like pili of the archaeon Methanococcus maripaludis
    Sandy Y M Ng
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    J Bacteriol 193:804-14. 2011
    The structure of pili from the archaeon Methanococcus maripaludis is unlike that of any bacterial pili. However, genetic analysis of the genes involved in the formation of these pili has been lacking until this study...
  18. pmc Identification of a putative acetyltransferase gene, MMP0350, which affects proper assembly of both flagella and pili in the archaeon Methanococcus maripaludis
    David J VanDyke
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Bacteriol 190:5300-7. 2008
    ..Recently, Methanococcus voltae flagellin proteins were found to have an N-linked trisaccharide necessary for proper flagellum assembly...
  19. pmc Characterization of energy-conserving hydrogenase B in Methanococcus maripaludis
    Tiffany A Major
    Department of Microbiology, University of Georgia, Athens, Georgia 30602 2605, USA
    J Bacteriol 192:4022-30. 2010
    The Methanococcus maripaludis energy-conserving hydrogenase B (Ehb) generates low potential electrons required for autotrophic CO(2) assimilation...
  20. pmc Dynamics of SecY translocons with translocation-defective mutations
    Ana Nicoleta Bondar
    Department of Physiology and Biophysics, University of California at Irvine, Irvine, CA 92697 4560, USA
    Structure 18:847-57. 2010
    ..The crystallographic structure of SecYEbeta from Methanococcus jannaschii revealed widespread distribution of mutations causing topogenesis defects, but not their molecular ..
  21. pmc Overlapping repressor binding sites regulate expression of the Methanococcus maripaludis glnK(1) operon
    Thomas J Lie
    Department of Microbiology, University of Washington, Box 357242, Seattle, WA 98195 7242, USA
    Mol Microbiol 75:755-62. 2010
    ..Particularly intriguing is the contrast between the nif and glnK(1) promoter regions of Methanococcus maripaludis, where two operators are present but with different configurations...
  22. pmc Autogenous translational regulation of the ribosomal MvaL1 operon in the archaebacterium Methanococcus vannielii
    M Hanner
    Institute of Medical Microbiology, University of Innsbruck, Austria
    J Bacteriol 176:409-18. 1994
    ..regulation of the synthesis of ribosomal proteins MvaL1, MvaL10, and MvaL12, encoded by the MvaL1 operon of Methanococcus vannielii, a methanogenic archaebacterium...
  23. ncbi Structure of the Methanococcus jannaschii mevalonate kinase, a member of the GHMP kinase superfamily
    Dong Yang
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843 2128 and Department of Chemistry, Texas A and M University, College Station, Texas 77843 3255
    J Biol Chem 277:9462-7. 2002
    ..The crystal structure of thermostable MVK from Methanococcus jannaschii has been determined at 2.4 A, revealing an overall fold similar to the homoserine kinase from M...
  24. ncbi New insights into the interaction of ribosomal protein L1 with RNA
    Natalia Nevskaya
    Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation
    J Mol Biol 355:747-59. 2006
    ..Incorporation of the structure of bacterial L1 into a model of the Escherichia coli ribosome revealed two additional contact regions for L1 on the 23S rRNA that were not identified in previous ribosome models...
  25. pmc Ammonia switch-off of nitrogen fixation in the methanogenic archaeon Methanococcus maripaludis: mechanistic features and requirement for the novel GlnB homologues, NifI(1) and NifI(2)
    P S Kessler
    Department of Microbiology, University of Washington, Seattle, Washington 98195, USA
    J Bacteriol 183:882-9. 2001
    ..We showed previously that in Methanococcus maripaludis switch-off requires at least one of two novel homologues of glnB, a family of genes whose products ..
  26. ncbi Activation mechanism of HSP16.5 from Methanococcus jannaschii
    Dong Ryoung Kim
    Department of Molecular Cell Biology, Center for Molecular Medicine, SBRI, Sungkyunkwan University School of Medicine, Suwon 440 746, South Korea
    Biochem Biophys Res Commun 307:991-8. 2003
    ..Although the crystal structure of HSP16.5, a small heat shock protein from Methanococcus jannaschii, revealed that it is a hollow sphere composed of 24 identical subunits, its activation mechanism ..
  27. pmc Genetics in methanogens: transposon insertion mutagenesis of a Methanococcus maripaludis nifH gene
    C E Blank
    Department of Microbiology, University of Washington, Seattle 98195
    J Bacteriol 177:5773-7. 1995
    We designed a transposon insertion mutagenesis system for Methanococcus species and used it to make mutations in and around a nifH gene in Methanococcus maripaludis...
  28. pmc Structural and functional differences between two homologous mechanosensitive channels of Methanococcus jannaschii
    A Kloda
    Department of Pharmacology, QEII Medical Centre, The University of Western Australia, Crawley, WA 6009, Australia
    EMBO J 20:1888-96. 2001
    ..cloning and characterization of MscMJLR, a second type of mechanosensitive (MS) channel found in the archaeon Methanococcus jannaschii. MscMJLR is structurally very similar to MscMJ, the MS channel of M...
  29. ncbi The active site of a lon protease from Methanococcus jannaschii distinctly differs from the canonical catalytic Dyad of Lon proteases
    Young Jun Im
    Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500 712, Korea
    J Biol Chem 279:53451-7. 2004
    ..b>Methanococcus jannaschii Lon (Mj-Lon) is a homologue of Escherichia coli Lon (Ec-Lon) but has two transmembrane helices ..
  30. ncbi Structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with divalent metal ions and the substrate ribulose 5-phosphate: implications for the catalytic mechanism
    Stefan Steinbacher
    Max Planck Institut fur Biochemie, Abteilung für Strukturforschung, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Biol Chem 278:42256-65. 2003
    ..Here, we present the crystal structure of Methanococcus jannaschii 3,4-dihydroxy-2-butanone 4-phosphate synthase in complex with the substrate ribulose 5-phosphate at ..
  31. ncbi Structural basis for recognition and catalysis by the bifunctional dCTP deaminase and dUTPase from Methanococcus jannaschii
    Joy L Huffman
    Department of Molecular Biology, The Scripps Research Institute, The Skaggs Institute for Chemical Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037 1027, USA
    J Mol Biol 331:885-96. 2003
    ..deaminase and dUTPase activities are usually found in separate but homologous enzymes, the hyperthermophile Methanococcus jannaschii has an enzyme, DCD-DUT, that harbors both dCTP deaminase and dUTP pyrophosphatase activities...
  32. pmc Rupture of the cell envelope by decompression of the deep-sea methanogen Methanococcus jannaschii
    Chan Beum Park
    Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA
    Appl Environ Microbiol 68:1458-63. 2002
    The effect of decompression on the structure of Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen, was studied in a novel high-pressure, high-temperature bioreactor. The cell envelope of M...
  33. ncbi Biosynthesis of riboflavin in archaea studies on the mechanism of 3,4-dihydroxy-2-butanone-4-phosphate synthase of Methanococcus jannaschii
    Markus Fischer
    Institut fur Organische Chemie und Biochemie, Technische Universitat Munchen, Lichtenbergstrasse 4, D 85747 Garching, Germany
    J Biol Chem 277:41410-6. 2002
    The hypothetical protein predicted by the open reading frame MJ0055 of Methanococcus jannaschii was expressed in a recombinant Escherichia coli strain under the control of a synthetic gene optimized for translation in an eubacterial host...
  34. pmc Conformational transition of Sec machinery inferred from bacterial SecYE structures
    Tomoya Tsukazaki
    Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama shi, Kanagawa 226 8501, Japan
    Nature 455:988-91. 2008
    ..The only high-resolution structure of a translocon available so far is that for SecYEbeta from the archaeon Methanococcus jannaschii, which lacks SecA. Here we present the 3...
  35. pmc Crystal structures of eukaryotic translation initiation factor 5A from Methanococcus jannaschii at 1.8 A resolution
    K K Kim
    Physical Biosciences Division of Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 95:10419-24. 1998
    ..The crystal structure of eIF-5A from Methanococcus jannaschii (MJ eIF-5A) has been determined at 1.9 A and 1...
  36. pmc Multiple states of a nucleotide-bound group 2 chaperonin
    Daniel K Clare
    Department of Crystallography, Birkbeck College and Institute of Structural Molecular Biology, University of London, London WC1E 7HX, United Kingdom
    Structure 16:528-34. 2008
    ..In contrast to the ordered apo state of the group 1 chaperonin GroEL, the unliganded form of the homo-16-mer Methanococcus maripaludis group 2 chaperonin is very open and flexible, with intersubunit contacts only in the central ..
  37. ncbi CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii
    Hong Li
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0308, USA
    Biochemistry 42:9771-8. 2003
    The protein product of the Methanococcus jannaschii MJ0768 gene has been expressed in Escherichia coli, purified to homogeneity, and shown to catalyze the GTP-dependent addition of two l-glutamates to the l-lactyl phosphodiester of 7,8-..
  38. ncbi Characterization of an 8-oxoguanine DNA glycosylase from Methanococcus jannaschii
    A Gogos
    Department of Biophysics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 274:30447-50. 1999
    A thermostable 8-oxoguanine (oxoG) DNA glycosylase from Methanococcus jannaschii has been expressed in Escherichia coli, purified, and characterized...
  39. ncbi Interaction of ribosomal L1 proteins from mesophilic and thermophilic Archaea and Bacteria with specific L1-binding sites on 23S rRNA and mRNA
    C Kohrer
    Institute of Medical Chemistry and Biochemistry, University of Innsbruck, Austria
    Eur J Biochem 256:97-105. 1998
    ..ribosomal L1 proteins from different archaeal and bacterial species, and 23S rRNA and mRNA fragments from Methanococcus vannielii containing the MvaL1-binding site. Under standard conditions (0 degrees C, pH 7...
  40. ncbi Development of genetic approaches for the methane-producing archaebacterium Methanococcus maripaludis
    W B Whitman
    Department of Microbiology, University of Georgia, Athens 30602 2605, USA
    Biofactors 6:37-46. 1997
    b>Methanococcus maripaludis is a strict anaerobe that utilizes H2 or formate as an electron donor for CO2 reduction to methane...
  41. pmc Genetic analysis of selenocysteine biosynthesis in the archaeon Methanococcus maripaludis
    Michael J Hohn
    Departments of Molecular Biophysics and Biochemistry Chemistry, Yale University, New Haven, CT 06520 8114, USA
    Mol Microbiol 81:249-58. 2011
    ..Here we present a mutational analysis of Sec biosynthesis in Methanococcus maripaludis strain Mm900...
  42. ncbi Selenium is involved in the negative regulation of the expression of selenium-free [NiFe] hydrogenases in Methanococcus voltae
    Y Berghöfer
    Department of Biology, Philipps University, Marburg, Germany
    Mol Gen Genet 242:369-73. 1994
    ..quantitatively the transcription patterns of the four different gene groups encoding [NiFe] hydrogenases in Methanococcus voltae...
  43. ncbi Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii
    Jens Hellmer
    Universitat Hannover, Institut fur Biophysik, Herrenhäuserstr 2, D 30419, Hannover, Germany
    FEBS Lett 527:245-9. 2002
    The genome of the hyperthermophilic archaeon Methanococcus jannaschii contains three Na(+)/H(+) antiporter related genes Mj0057, Mj1521 and Mj1275...
  44. ncbi A lysR-type regulator is involved in the negative regulation of genes encoding selenium-free hydrogenases in the archaeon Methanococcus voltae
    Junsong Sun
    Genetics, Department of Biology, Philipps University of Marburg, D 35032 Marburg, Germany
    Mol Microbiol 52:563-71. 2004
    The archaeon Methanococcus voltae encodes two pairs of NiFe-hydrogenase isoenzymes. One hydrogenase of each pair contains selenium in the active site, whereas the other one is selenium-free...
  45. ncbi Methanococcus vulcanius sp. nov., a novel hyperthermophilic methanogen isolated from East Pacific Rise, and identification of Methanococcus sp. DSM 4213T as Methanococcus fervens sp. nov
    C Jeanthon
    Station Biologique, UPR9042, Centre National de la Recherche Scientifique, Roscoff, France
    Int J Syst Bacteriol 49:583-9. 1999
    ..As determined by 16S rDNA gene sequence analysis, this organism was closely related to Methanococcus jannaschii strain JAL-1T...
  46. pmc The Sac10b homolog in Methanococcus maripaludis binds DNA at specific sites
    Yuchen Liu
    Departments of Microbiology, University of Georgia, Athens, 30602, USA
    J Bacteriol 191:2315-29. 2009
    ..Mma10b from the euryarchaeote Methanococcus maripaludis is a mesophilic member of the Sac10b family...
  47. pmc Expression vectors for Methanococcus maripaludis: overexpression of acetohydroxyacid synthase and beta-galactosidase
    W L Gardner
    Department of Microbiology, University of Georgia, Athens, Georgia 30602 2605, USA
    Genetics 152:1439-47. 1999
    A series of integrative and shuttle expression vectors was developed for use in Methanococcus maripaludis...
  48. pmc Characterization of pURB500 from the archaeon Methanococcus maripaludis and construction of a shuttle vector
    D L Tumbula
    Department of Microbiology, University of Georgia, Athens 30602 2605, USA
    J Bacteriol 179:2976-86. 1997
    The complete sequence of the 8,285-bp plasmid pURB500 from Methanococcus maripaludis C5 was determined. Sequence analysis identified 18 open reading frames as well as two regions of potential iterons and complex secondary structures...
  49. pmc Biosynthesis of 4-thiouridine in tRNA in the methanogenic archaeon Methanococcus maripaludis
    Yuchen Liu
    Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 287:36683-92. 2012
    ..Using the methanogenic archaeon Methanococcus maripaludis, we show that deletion of ThiI (MMP1354) abolished the biosynthesis of s(4)U but not of thiamine...
  50. pmc Methanococcus vannielii selenium-binding protein (SeBP): chemical reactivity of recombinant SeBP produced in Escherichia coli
    Kemberly G Patteson
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 50 Room 2120, 50 South Drive, Bethesda, MD 20892 8012, USA
    Proc Natl Acad Sci U S A 102:12029-34. 2005
    A selenium-binding protein (SeBP) from Methanococcus vannielii was recently identified, and its gene was isolated and overexpressed in Escherichia coli [Self, W. T., Pierce, R. & Stadtman, T. C. (2004) IUBMB Life 56, 501-507]...
  51. ncbi Subunit exchange, conformational stability, and chaperone-like function of the small heat shock protein 16.5 from Methanococcus jannaschii
    Michael P Bova
    Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, California 90095, USA
    J Biol Chem 277:38468-75. 2002
    Hsp16.5, isolated from the hyperthermophilic Archaea Methanococcus jannaschii, is a member of the small heat-shock protein family. Small Hsps have 12- to 42-kDa subunit sizes and have sequences that are conserved among all organisms...
  52. ncbi Methanococcus jannaschii ORF mj0608 codes for a class C inorganic pyrophosphatase protected by Co(2+) or Mn(2+) ions against fluoride inhibition
    N J Kuhn
    School of Biosciences, The University of Birmingham, Birmingham, B15 2TT, United Kingdom
    Arch Biochem Biophys 379:292-8. 2000
    Openreading frame mj0608 of the Methanococcus jannaschii genome, recognized by its sequence similarity to that of the gene coding for class C inorganic pyrophosphatase in Bacillus subtilis, was cloned and over-expressed in Escherichia ..
  53. doi Disruption and complementation of the selenocysteine biosynthesis pathway reveals a hierarchy of selenoprotein gene expression in the archaeon Methanococcus maripaludis
    Tilmann Stock
    Institut für Molekulare Biowissenschaften, Johann Wolfgang Goethe Universitat, Frankfurt am Main, Germany
    Mol Microbiol 82:734-47. 2011
    ..An Escherichia coli strain lacking the gene for tRNA(sec) could only be complemented with the homologue from Methanococcus maripaludis when a single base in the anticodon loop was exchanged demonstrating that this base is a crucial ..
  54. ncbi Mutational analysis of genes encoding chromatin proteins in the archaeon Methanococcus voltae indicates their involvement in the regulation of gene expression
    I Heinicke
    Fachbereich Biologie Genetik, Philipps Universitat Marburg, Karl v Frisch Str 8, 35043 Marburg, Germany
    Mol Genet Genomics 272:76-87. 2004
    ..The methanogenic archaeon Methanococcus voltae harbors four genes coding for all these three types of chromatin proteins...
  55. ncbi Crystal structure determination of FtsZ from Methanococcus jannaschii
    J Lowe
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    J Struct Biol 124:235-43. 1998
    ..Full-length FtsZ1 from Methanococcus jannaschii has been over expressed in Escherichia coli, employing the hyperthermophilic properties of the ..
  56. ncbi Structure and function of the FeoB G-domain from Methanococcus jannaschii
    Stefan Köster
    Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany
    J Mol Biol 392:405-19. 2009
    ..We determined high-resolution X-ray structures of the FeoB G-domain from Methanococcus jannaschii with and without bound GDP or Mg(2+)-GppNHp...
  57. ncbi Metal ion binding and enzymatic mechanism of Methanococcus jannaschii RNase HII
    Bing Lai
    State Key Laboratory for Structural Chemistry Studies of Stable and Unstable Species, Institute of Physical Chemistry, College of Chemistry, Peking University, Beijing 100871, China
    Biochemistry 42:785-91. 2003
    ..have studied the thermodynamic properties of four metal ions (Mg(2+), Mn(2+), Ca(2+), and Ba(2+)) binding to Methanococcus jannaschii RNase HII using isothermal titration calorimetry...
  58. ncbi Structure-based identification of a novel NTPase from Methanococcus jannaschii
    K Y Hwang
    Structural Biology Center, Korea Institute of Science and Technology, Seoul, South Korea
    Nat Struct Biol 6:691-6. 1999
    Almost half of the entire set of predicted genomic products from Methanococcus jannaschii are classified as functionally unknown hypothetical proteins...
  59. pmc Nitrogenase phylogeny and the molybdenum dependence of nitrogen fixation in Methanococcus maripaludis
    P S Kessler
    Department of Microbiology, University of Washington, Seattle 98195, USA
    J Bacteriol 179:541-3. 1997
    We studied the effects of molybdenum, vanadium, and tungsten on the diazotrophic growth of Methanococcus maripaludis. Mo stimulated growth, with a maximal response at 4.0 microM, while V had no effect at any concentration tested...
  60. ncbi Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock
    Boonchai B Boonyaratanakornkit
    Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA
    Environ Microbiol 7:789-97. 2005
    Temperature shock of the hyperthermophilic methanarchaeon Methanococcus jannaschii from its optimal growth temperature of 85 degrees C to 65 degrees C and 95 degrees C resulted in different transcriptional responses characteristic of ..
  61. doi Methanococcus maripaludis: an archaeon with multiple functional MCM proteins?
    Alison D Walters
    Department of Biology Area 5, PO Box 373, University of York, York YO10 5YW, UK
    Biochem Soc Trans 37:1-6. 2009
    ..Unusually, the genome of the methanogenic archaeon Methanococcus maripaludis contains four MCM homologues, all of which contain the conserved motifs required for function...
  62. pmc Cysteine is not the sulfur source for iron-sulfur cluster and methionine biosynthesis in the methanogenic archaeon Methanococcus maripaludis
    Yuchen Liu
    Department of Microbiology, University of Georgia, Athens, Georgia 30602 2605, USA
    J Biol Chem 285:31923-9. 2010
    ..b>Methanococcus maripaludis is a methanogenic archaeon that contains a high amount of protein-bound Fe-S clusters (45 nmol/mg ..
  63. doi Cloning, expression and purification of DNA-binding protein Mvo10b from Methanococcus voltae
    Jinsong Xuan
    Dept of Biological Science and Technology, University of Science and Technology Beijing, 30 Xueyuan Road, Beijing 100083, China
    Protein Expr Purif 64:162-6. 2009
    Mvo10b from the mesophilic archaeon Methanococcus voltae is a member of the Sac10b family which may play an important role in the organization and accessibility of genetic information in Archaea...
  64. ncbi Crystal structure of phosphoserine phosphatase from Methanococcus jannaschii, a hyperthermophile, at 1.8 A resolution
    W Wang
    Department of Chemistry, University of California, Berkeley, 94720, USA
    Structure 9:65-71. 2001
    ..The strong resemblance in sequence and mechanism implies structural similarity among these enzymes...
  65. pmc Reactor-scale cultivation of the hyperthermophilic methanarchaeon Methanococcus jannaschii to high cell densities
    B Mukhopadhyay
    Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Appl Environ Microbiol 65:5059-65. 1999
    For the hyperthermophilic and barophilic methanarchaeon Methanococcus jannaschii, we have developed a medium and protocols for reactor-scale cultivation that improved the final cell yield per liter from approximately 0...
  66. ncbi The crystal structure of flap endonuclease-1 from Methanococcus jannaschii
    K Y Hwang
    Structural Biology Center, Korea Institute of Science and Technology, Seoul, South Korea
    Nat Struct Biol 5:707-13. 1998
    ..The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2...
  67. pmc An Hfq-like protein in archaea: crystal structure and functional characterization of the Sm protein from Methanococcus jannaschii
    Jesper S Nielsen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    RNA 13:2213-23. 2007
    ..there are generally only one or two subtypes of Sm-related proteins in archaea, but at least one archaeon, Methanococcus jannaschii, encodes a protein that is related to Hfq...
  68. pmc Archaeal phylogeny based on proteins of the transcription and translation machineries: tackling the Methanopyrus kandleri paradox
    Celine Brochier
    Equipe Phylogénomique, Université Aix Marseille I, Centre Saint Charles, 13331 Marseille Cedex 3, France
    Genome Biol 5:R17. 2004
    ..We analyzed archaeal phylogeny using two concatenated datasets consisting of 14 proteins involved in transcription and 53 ribosomal proteins (3,275 and 6,377 positions, respectively)...
  69. ncbi Prophage-like gene transfer agents-novel mechanisms of gene exchange for Methanococcus, Desulfovibrio, Brachyspira, and Rhodobacter species
    Thad B Stanton
    Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2300 Dayton Road, Ames, IA 50010, USA
    Anaerobe 13:43-9. 2007
    ..4 to 13.6kb. GTAs have been reported in species of Brachyspira, Methanococcus, Desulfovibrio, and Rhodobacter...
  70. pmc Noncoding RNA genes identified in AT-rich hyperthermophiles
    Robert J Klein
    Howard Hughes Medical Institute and Department of Genetics, Washington University School of Medicine, Saint Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 99:7542-7. 2002
    ..bias in genomes of some hyperthermophilic organisms: simply screening for GC-rich regions in the AT-rich Methanococcus jannaschii and Pyrococcus furiosus genomes efficiently detects both known and new RNA genes with a high degree ..
  71. pmc Impact of translational selection on codon usage bias in the archaeon Methanococcus maripaludis
    Laura R Emery
    Institute of Evolutionary Biology, University of Edinburgh, King s Buildings, Edinburgh EH9 3JT, UK
    Biol Lett 7:131-5. 2011
    ..Here, we examine the nature of codon usage bias in a methanogenic archaeon, Methanococcus maripaludis...
  72. pmc Functionally distinct genes regulated by hydrogen limitation and growth rate in methanogenic Archaea
    Erik L Hendrickson
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 104:8930-4. 2007
    ..Cells of Methanococcus maripaludis were grown by using continuous culture to deconvolute the effects of hydrogen limitation and ..
  73. ncbi Crystal structure of the antitoxin-toxin protein complex RelB-RelE from Methanococcus jannaschii
    Djordje Francuski
    Kristallographie, Institut fur Chemie und Biochemie, Freie Universitat Berlin, Takustrasse 6, Berlin 14195, Germany
    J Mol Biol 393:898-908. 2009
    Here we present the crystal structure of the Methanococcus jannaschii RelE-RelB (RelBE) toxin-antitoxin (TA) protein complex determined by the MIRAS (multiple isomorphous replacement with anomalous signal) method...
  74. pmc A thermostable platform for transcriptional regulation: the DNA-binding properties of two Lrp homologs from the hyperthermophilic archaeon Methanococcus jannaschii
    M Ouhammouch
    Division of Biology and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    EMBO J 20:146-56. 2001
    The hyperthermophilic archaeon Methanococcus jannaschii encodes two putative transcription regulators, Ptr1 and Ptr2, related to the bacterial Lrp/AsnC family of transcriptional regulators...
  75. pmc Archaeal RNA polymerase subunits F and P are bona fide homologs of eukaryotic RPB4 and RPB12
    F Werner
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, Exhibition Road, London SW7 2AY, UK
    Nucleic Acids Res 28:4299-305. 2000
    ..We prepared recombinant versions of the F and P subunits from Methanococcus jannaschii and used them in in vitro and in vivo protein interaction assays to demonstrate that they interact ..
  76. pmc Solution nuclear magnetic resonance structure of a protein disulfide oxidoreductase from Methanococcus jannaschii
    J W Cave
    Department of Chemistry, University of California at Berkeley, Berkeley, California 94720 1460, USA
    Protein Sci 10:384-96. 2001
    ..The secondary and tertiary structure of this protein from the archaebacterium Methanococcus jannaschii is similar to the structures that have been solved for the glutaredoxin proteins from Escherichia ..
  77. ncbi Structure of ribosomal protein L1 from Methanococcus thermolithotrophicus. Functionally important structural invariants on the L1 surface
    Natalia Nevskaya
    Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia
    Acta Crystallogr D Biol Crystallogr 58:1023-9. 2002
    The crystal structure of ribosomal protein L1 from the archaeon Methanococcus thermolithotrophicus has been determined at 2.7 A resolution. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 67.0, b = 70...
  78. ncbi Detailed analysis of RNA-protein interactions within the ribosomal protein S8-rRNA complex from the archaeon Methanococcus jannaschii
    S Tishchenko
    Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia
    J Mol Biol 311:311-24. 2001
    The crystal structure of ribosomal protein S8 bound to its target 16 S rRNA from a hyperthermophilic archaeon Methanococcus jannaschii has been determined at 2.6 A resolution...
  79. ncbi Divergent adaptation of tRNA recognition by Methanococcus jannaschii prolyl-tRNA synthetase
    B Burke
    Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 276:20286-91. 2001
    ..The archaeal Methanococcus jannaschii ProRS is a member of the eukaryotic-like group, although its tRNA(Pro) possesses prokaryotic ..
  80. pmc Structure and dynamics of translation initiation factor aIF-1A from the archaeon Methanococcus jannaschii determined by NMR spectroscopy
    W Li
    Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, 78712, USA
    Protein Sci 10:2426-38. 2001
    Translation initiation factor 1A (aIF-1A) from the archaeon Methanococcus jannaschii was expressed in Escherichia coli, purified, and characterized in terms of its structure and dynamics using multidimensional NMR methods...
  81. pmc Site-directed mutagenesis analysis of amino acids critical for activity of the type I signal peptidase of the archaeon Methanococcus voltae
    Sonia L Bardy
    Department of Microbiology and Immunology, Queen s University, Kingston, ON, Canada K7L 3N6
    J Bacteriol 187:1188-91. 2005
    Site-directed mutagenesis studies of the signal peptidase of the methanogenic archaeon Methanococcus voltae identified three conserved residues (Ser52, His122, and Asp148) critical for activity...
  82. ncbi Pyruvoyl-dependent arginine decarboxylase from Methanococcus jannaschii: crystal structures of the self-cleaved and S53A proenzyme forms
    W David Tolbert
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
    Structure 11:285-94. 2003
    The three-dimensional structure of pyruvoyl-dependent arginine decarboxylase from Methanococcus jannaschii was determined at 1.4 A resolution...
  83. pmc Quantitative proteomics of the archaeon Methanococcus maripaludis validated by microarray analysis and real time PCR
    Qiangwei Xia
    Department of Chemical Engineering, University of Washington, Seattle, Washington 98195, USA
    Mol Cell Proteomics 5:868-81. 2006
    For the archaeon Methanococcus maripaludis, a fully sequenced and annotated model species of hydrogenotrophic methanogen, we report validation of quantitative protein level expression ratios on a proteome-wide basis...
  84. pmc RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea
    Jing Yuan
    Department of Molecular Biophysics and Biochemistry and Chemistry, Yale University, New Haven, CT 06520 8114, USA
    Proc Natl Acad Sci U S A 103:18923-7. 2006
    ..SepSecS and PSTK coevolved with the archaeal and eukaryotic lineages, but the history of PSTK is marked by several horizontal gene transfer events, including transfer to non-Sec-decoding Cyanobacteria and fungi...
  85. pmc Disruption of the operon encoding Ehb hydrogenase limits anabolic CO2 assimilation in the archaeon Methanococcus maripaludis
    Iris Porat
    Department of Microbiology, University of Georgia, Athens, Georgia 30602 2605, USA
    J Bacteriol 188:1373-80. 2006
    b>Methanococcus maripaludis is a mesophilic archaeon that reduces CO2 to methane with H2 or formate as an energy source. It contains two membrane-bound energy-conserving hydrogenases, Eha and Ehb...
  86. ncbi RNA-dependent cysteine biosynthesis in archaea
    Anselm Sauerwald
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Science 307:1969-72. 2005
    ..This was proven for Methanococcus maripaludis, where deletion of the SepRS-encoding gene resulted in cysteine auxotrophy...
  87. ncbi A fully recombinant system for activator-dependent archaeal transcription
    Mohamed Ouhammouch
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
    J Biol Chem 279:51719-21. 2004
    ....
  88. ncbi Continuous culture of Methanococcus maripaludis under defined nutrient conditions
    Andrew K Haydock
    Department of Microbiology, University of Washington, P O Box 357242, Seattle, WA 98195 7242, USA
    FEMS Microbiol Lett 238:85-91. 2004
    To study global regulation in the methanogenic archaeon Methanococcus maripaludis, we devised a system for steady-state growth in chemostats...
  89. ncbi Structure and subunit arrangement of the A-type ATP synthase complex from the archaeon Methanococcus jannaschii visualized by electron microscopy
    Unal Coskun
    Universitat des Saarlandes, Fachrichtung 2 5 Biophysik, D 66421 Homburg, Germany
    J Biol Chem 279:38644-8. 2004
    ..S. A. 100, 2312-2315). Here, we report the first projected structure of an intact A(1)A(0) ATP synthase from Methanococcus jannaschii as determined by electron microscopy and single particle analysis at a resolution of 1.8 nm...
  90. pmc Characterization of the 3' exonuclease subunit DP1 of Methanococcus jannaschii replicative DNA polymerase D
    Maarit Jokela
    Biocenter Oulu and Department of Biochemistry, PO Box 3000, FIN 90014 University of Oulu, Finland
    Nucleic Acids Res 32:2430-40. 2004
    ..To test this hypothesis we expressed Methanococcus jannaschii DP1 (MjaDP1), the B-subunit of DNA polymerase D, in Escherichia coli, and demonstrate that MjaDP1 ..
  91. pmc Global responses of Methanococcus maripaludis to specific nutrient limitations and growth rate
    Erik L Hendrickson
    University of Washington, Department of Microbiology, Box 357242, Seattle, WA 98195 7242, USA
    J Bacteriol 190:2198-205. 2008
    ..and measurements of cellular amino acid pools and tRNA charging levels were used to determine the response of Methanococcus maripaludis to leucine limitation...
  92. ncbi Cleavage of preflagellins by an aspartic acid signal peptidase is essential for flagellation in the archaeon Methanococcus voltae
    Sonia L Bardy
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Mol Microbiol 50:1339-47. 2003
    ..The enzyme responsible for the removal of the flagellin leader peptide was identified as FlaK. FlaK of Methanococcus voltae retains its preflagellin peptidase activity when expressed in Escherichia coli and used in an in vitro ..
  93. ncbi Topography of the euryarchaeal transcription initiation complex
    Michael S Bartlett
    Division of Biological Sciences, Center for Molecular Genetics, University of California at San Diego, La Jolla, California 92093 0634, USA
    J Biol Chem 279:5894-903. 2004
    ..The location of this protein suggests that it, like TFB1, could also have a role in transcription initiation following RNA polymerase recruitment...
  94. pmc Quantitative proteomics of nutrient limitation in the hydrogenotrophic methanogen Methanococcus maripaludis
    Qiangwei Xia
    Department of Chemical Engineering, Box 355014, University of Washington, Seattle, WA 98195, USA
    BMC Microbiol 9:149. 2009
    Methanogenic Archaea play key metabolic roles in anaerobic ecosystems, where they use H2 and other substrates to produce methane. Methanococcus maripaludis is a model for studies of the global response to nutrient limitations.
  95. ncbi Expression and purification of Arg196 and Lys272 mutants of mevalonate kinase from Methanococcus jannaschii
    Xiusheng Chu
    Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, PR China
    Protein Expr Purif 30:210-8. 2003
    ..We subcloned the gene of mevalonate kinase from Methanococcus jannaschii into a bacterial expression vector pLM1 with six continuous histidine codons attached to the 5' end ..
  96. ncbi Asp302 determines potassium dependence of a RadA recombinase from Methanococcus voltae
    Xinguo Qian
    Department of Biochemistry, University of Saskatchewan, A3 Health Sciences Building, 107 Wiggins Road, Saskatoon, Saskatchewan, Canada S7N 5E5
    J Mol Biol 360:537-47. 2006
    ..Our recent ATPase and structure studies on RadA recombinase from Methanococcus voltae have suggested that not only magnesium but also potassium ions are absorbed at the ATPase center...
  97. ncbi Structures of thermophilic and mesophilic adenylate kinases from the genus Methanococcus
    Angela R Criswell
    Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA
    J Mol Biol 330:1087-99. 2003
    The crystal structures of adenylate kinases from the thermophile Methanococcus thermolithotrophicus and the mesophile Methanococcus voltae have been solved to resolutions of 2.8A and 2.5A, respectively...
  98. pmc The Thermotoga maritima phenotype is impacted by syntrophic interaction with Methanococcus jannaschii in hyperthermophilic coculture
    M R Johnson
    Dept of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695 7905, USA
    Appl Environ Microbiol 72:811-8. 2006
    ..the hyperthermophilic bacterium Thermotoga maritima when it was cocultured with the hyperthermophilic archaeon Methanococcus jannaschii. For the mid-log-to-early-stationary-phase transition of a T. maritima monoculture, 24 genes (1...
  99. ncbi A stable archaeal pyruvate carboxylase from the hyperthermophile Methanococcus jannaschii
    B Mukhopadhyay
    Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, 61801, USA
    Arch Microbiol 174:406-14. 2000
    ..carboxylase (PYC) of the hyperthermophilic, strictly hydrogenotrophic, autotrophic and marine methanarchaeon Methanococcus jannaschii was purified to homogeneity. Optimal activity was at pH 8...
  100. ncbi Crystal structure and evolution of a transfer RNA splicing enzyme
    H Li
    Division of Biology, Mail Code 147 75, California Institute of Technology, Pasadena, CA 91125, USA
    Science 280:279-84. 1998
    ..The crystal structure of the endonuclease from the archaeon Methanococcus jannaschii was determined to a resolution of 2.3 angstroms...
  101. ncbi Crystal structure of a dimeric archaeal splicing endonuclease
    H Li
    Division of Biology, California Institute of Technology, Pasadena, CA, USA
    J Mol Biol 302:639-48. 2000
    ..to that of a single subunit of the previously determined homotetrameric tRNA splicing endonuclease from Methanococcus jannaschii (MJ), indicating its role in catalysis...

Research Grants85

  1. Archaeal gene recruitment by the Chlamydiales
    David Graham; Fiscal Year: 2007
    ..of a pyruvoyl-dependent arginine decarboxylase gene, previously identified in the non-pathogenic archaeon Methanococcus jannaschii...
  2. Aminoacyl-tRNA synthetases in Archaea
    Ya Ming Hou; Fiscal Year: 2003
    ..For example, recent studies have identified a dual specific prolyl-tRNA synthetase (ProRS) of the archaeon Methanococcus jannaschii, which has the ability to activate both proline and cysteine and catalyze aminoacylation of tRNA ..
  3. SERYL-TRNA SYNTHETASES: EVOLUTION AND DIVERSITY
    Dieter Soll; Fiscal Year: 2001
    ..The sequencing of the first archaeal genome (Methanococcus jannaschii) revealed the "lack" of few canonical aminoacyl-tRNA synthetases, including the one specific for ..
  4. SERYL-TRNA SYNTHETASES: EVOLUTION AND DIVERSITY
    Dieter Soll; Fiscal Year: 2002
    ..The sequencing of the first archaeal genome (Methanococcus jannaschii) revealed the "lack" of few canonical aminoacyl-tRNA synthetases, including the one specific for ..
  5. SERYL-TRNA SYNTHETASES: EVOLUTION AND DIVERSITY
    Dieter Soll; Fiscal Year: 2003
    ..The sequencing of the first archaeal genome (Methanococcus jannaschii) revealed the "lack" of few canonical aminoacyl-tRNA synthetases, including the one specific for ..
  6. Membrane Protein Folding and Assembly
    Stephen H White; Fiscal Year: 2011
    ..We have discovered through molecular dynamics simulations of the SecYEG translocon from Methanococcus jannaschii, whose 3D structure is known, that the translocon is stabilized in its closed state by an intricate ..
  7. FEN1 STRUCTURES AND HUMAN GENOME STABILITY
    John Tainer; Fiscal Year: 2000
    ..and then used to solve pFEN-1 mutant structures as well as possible human, yeast, Archaeglobis fulgidus and Methanococcus jannaschii FEN-1 structures...
  8. FEN1 STRUCTURES AND HUMAN GENOME STABILITY
    John Tainer; Fiscal Year: 1999
    ..and then used to solve pFEN-1 mutant structures as well as possible human, yeast, Archaeglobis fulgidus and Methanococcus jannaschii FEN-1 structures...
  9. FEN1 STRUCTURES AND HUMAN GENOME STABILITY
    John Tainer; Fiscal Year: 2002
    ..and then used to solve pFEN-1 mutant structures as well as possible human, yeast, Archaeglobis fulgidus and Methanococcus jannaschii FEN-1 structures...
  10. FEN1 STRUCTURES AND HUMAN GENOME STABILITY
    John Tainer; Fiscal Year: 2001
    ..and then used to solve pFEN-1 mutant structures as well as possible human, yeast, Archaeglobis fulgidus and Methanococcus jannaschii FEN-1 structures...
  11. PROTEIN TRANSLOCATION ACROSS THE ENDOPLASMIC RETICULUM
    JAMES REID GILMORE; Fiscal Year: 2010
    ..The structure of the Methanococcus jannaschii SecYE2 complex provides an excellent model for the closed conformation of a eukaryotic protein ..
  12. STRUCTURAL STUDIES OF METHYL CYCLE ENZYMES
    Fusao Takusagawa; Fiscal Year: 2003
    ..S-adenosylm ethionine synthetase from human, rat, Methanococcus jannaschii, and E. coli. Glycline N-methyltransferase and guanidinoacetate methyltransferase from rat liver...
  13. STRUCTURAL STUDIES OF METHYL CYCLE ENZYMES
    Fusao Takusagawa; Fiscal Year: 2001
    ..S-adenosylm ethionine synthetase from human, rat, Methanococcus jannaschii, and E. coli. Glycline N-methyltransferase and guanidinoacetate methyltransferase from rat liver...
  14. STRUCTURAL STUDIES OF METHYL CYCLE ENZYMES
    Fusao Takusagawa; Fiscal Year: 2000
    ..S-adenosylm ethionine synthetase from human, rat, Methanococcus jannaschii, and E. coli. Glycline N-methyltransferase and guanidinoacetate methyltransferase from rat liver...
  15. STRUCTURAL STUDIES OF METHYL CYCLE ENZYMES
    Fusao Takusagawa; Fiscal Year: 2002
    ..S-adenosylm ethionine synthetase from human, rat, Methanococcus jannaschii, and E. coli. Glycline N-methyltransferase and guanidinoacetate methyltransferase from rat liver...
  16. High Resolution Structural Dyanmics of K Channels
    EDUARDO A PEROZO; Fiscal Year: 2010
    ..Phosphatase (Ci-VSP) and the hyperpolarization-activated six-transmembrane segment (6TM) channel from Methanococcus janschii (MVP)...
  17. ENZYMATIC MECHANISMS OF SULFUR NUCLEOSIDE METABOLISM
    GEORGE MARKHAM; Fiscal Year: 2000
    ..very different sequences from those of the eukarya and prokarya, suggesting an altered catalytic strategy; the Methanococcus jannaschii synthetase will be characterized...
  18. ENZYMATIC MECHANISMS OF SULFUR NUCLEOSIDE METABOLISM
    GEORGE MARKHAM; Fiscal Year: 2003
    ..very different sequences from those of the eukarya and prokarya, suggesting an altered catalytic strategy; the Methanococcus jannaschii synthetase will be characterized...
  19. ENZYMATIC MECHANISMS OF SULFUR NUCLEOSIDE METABOLISM
    GEORGE MARKHAM; Fiscal Year: 2002
    ..very different sequences from those of the eukarya and prokarya, suggesting an altered catalytic strategy; the Methanococcus jannaschii synthetase will be characterized...
  20. ENZYMATIC MECHANISMS OF SULFUR NUCLEOSIDE METABOLISM
    GEORGE MARKHAM; Fiscal Year: 2001
    ..very different sequences from those of the eukarya and prokarya, suggesting an altered catalytic strategy; the Methanococcus jannaschii synthetase will be characterized...
  21. Structure Studies of Processive DNA Replication
    John Kuriyan; Fiscal Year: 2007
    ..coli, the thermophilic eubacterium Aquifex aeolicus, the archaeon Methanococcus janschii and the eukaryote S. cerevisiae...
  22. Structure Studies of Processive DNA Replication
    John Kuriyan; Fiscal Year: 2009
    ..coli, the thermophilic eubacterium Aquifex aeolicus, the archaeon Methanococcus janschii and the eukaryote S. cerevisiae...
  23. Structure Studies of Processive DNA Replication
    John Kuriyan; Fiscal Year: 2010
    ..coli, the thermophilic eubacterium Aquifex aeolicus, the archaeon Methanococcus janschii and the eukaryote S. cerevisiae...
  24. Investigation of Noncanonical Cysteine tRNA Synthetases
    Kirk Beebe; Fiscal Year: 2004
    ..Three members from this unique group were revealed from the Methanococcus jannaschii, Deinococcus radiodurans, and Thermotoga maritima...
  25. Nuclear Magnetic Resonance Studies of Proteins
    ARTHUR PALMER; Fiscal Year: 2005
    ..binding by the ATP-binding cassette (ABC) MJ1267 of the branched chain amino acid (LIV) transporter from Methanococcus jannaschii. GCN4 is the prototypical member of the bZip family of transcription activators...
  26. Nuclear Magnetic Resonance Studies of Structures and Dynamics of Proteins
    ARTHUR PALMER; Fiscal Year: 2006
    ..binding by the ATP-binding cassette (ABC) MJ1267 of the branched chain amino acid (LIV) transporter from Methanococcus jannaschii. GCN4 is the prototypical member of the bZip family of transcription activators...
  27. Nuclear Magnetic Resonance Studies of Structures and Dynamics of Proteins
    ARTHUR PALMER; Fiscal Year: 2007
    ..binding by the ATP-binding cassette (ABC) MJ1267 of the branched chain amino acid (LIV) transporter from Methanococcus jannaschii. GCN4 is the prototypical member of the bZip family of transcription activators...
  28. Nuclear Magnetic Resonance Studies of Structures and Dynamics of Proteins
    ARTHUR PALMER; Fiscal Year: 2009
    ..binding by the ATP-binding cassette (ABC) MJ1267 of the branched chain amino acid (LIV) transporter from Methanococcus jannaschii. GCN4 is the prototypical member of the bZip family of transcription activators...
  29. High Resolution Sructural Dynamics of K Channels
    Eduardo Perozo; Fiscal Year: 2007
    ..attention on a newly characterized hyperpolarization-activated six-transmembrane segment (6TM) channel from Methanococcus janschii (MVP)...
  30. High Resolution Sructural Dynamics of K Channels
    Eduardo Perozo; Fiscal Year: 2009
    ..attention on a newly characterized hyperpolarization-activated six-transmembrane segment (6TM) channel from Methanococcus janschii (MVP)...
  31. High Resolution Sructural Dynamics of K Channels
    Eduardo Perozo; Fiscal Year: 2006
    ..attention on a newly characterized hyperpolarization-activated six-transmembrane segment (6TM) channel from Methanococcus janschii (MVP)...
  32. Methanococcus maripaludis as an emerging model organism
    John A Leigh; Fiscal Year: 2010
    b>Methanococcus maripaludis is emerging as a premier model for the methanogens and the Archaea. It grows unusually well, has an extensive set of facile genetic tools, and has a known complete annotated genome sequence...
  33. Methanococcus maripaludis as an emerging model organism
    JOHN LEIGH; Fiscal Year: 2009
    b>Methanococcus maripaludis is emerging as a premier model for the methanogens and the Archaea. It grows unusually well, has an extensive set of facile genetic tools, and has a known complete annotated genome sequence...
  34. Methanococcus maripaludis as an emerging model organism
    John A Leigh; Fiscal Year: 2010
    b>Methanococcus maripaludis is emerging as a premier model for the methanogens and the Archaea. It grows unusually well, has an extensive set of facile genetic tools, and has a known complete annotated genome sequence...
  35. HIGH RESOLUTION STRUCTURAL DYNAMICS OF K CHANNELS
    Eduardo Perozo; Fiscal Year: 2003
    ..In addition, we will focus our attention on a newly characterized six-transmembrane segment (6TM) channel from Methanococcus janschii (which we have named KchV-O)...
  36. HIGH RESOLUTION STRUCTURAL DYNAMICS OF K CHANNELS
    Eduardo Perozo; Fiscal Year: 2005
    ..In addition, we will focus our attention on a newly characterized six-transmembrane segment (6TM) channel from Methanococcus janschii (which we have named KchV-O)...
  37. HIGH RESOLUTION STRUCTURAL DYNAMICS OF K CHANNELS
    Eduardo Perozo; Fiscal Year: 2005
    ..In addition, we will focus our attention on a newly characterized six-transmembrane segment (6TM) channel from Methanococcus janschii (which we have named KchV-O)...
  38. HIGH RESOLUTION STRUCTURAL DYNAMICS OF K CHANNELS
    Eduardo Perozo; Fiscal Year: 2004
    ..In addition, we will focus our attention on a newly characterized six-transmembrane segment (6TM) channel from Methanococcus janschii (which we have named KchV-O)...
  39. HIGH RESOLUTION STRUCTURAL DYNAMICS OF K CHANNELS
    Eduardo Perozo; Fiscal Year: 2002
    ..In addition, we will focus our attention on a newly characterized six-transmembrane segment (6TM) channel from Methanococcus janschii (which we have named KchV-O)...
  40. SPECTROSCOPIC STUDIES OF HYDROGENASE MODELS
    Michael Maroney; Fiscal Year: 2000
    ..Upon successful completion of the model studies, enzyme samples will be prepared by supplementing the growth medium of the bacteria involved (e.g., Desulfomicrobiurn baculaturn or Methanococcus voltae) with 77Se.
  41. CHARACTERIZATION OF METHANOCOCCUS VOLTAE ATPASE
    Jordan Konisky; Fiscal Year: 1993
    ..is a continuation of our efforts to characterize membrane function and energetic in the archaebacterium Methanococcus voltae. This methanogen is a hydrogen-oxidizing obligate anaerobe which requires sodium. Studies of M...
  42. CHARACTERIZATION OF METHANOCOCCUS VOLTAE ATPASE
    Jordan Konisky; Fiscal Year: 1990
    ..is a continuation of our efforts to characterize membrane function and energetic in the archaebacterium Methanococcus voltae. This methanogen is a hydrogen-oxidizing obligate anaerobe which requires sodium. Studies of M...
  43. PREFOLDING AND PROTEIN FOLDING
    NICHOLAS COWAN; Fiscal Year: 2000
    ..We will coexpress the two prefoldin subunit homologs from the archaeon Methanococcus jannaschii in E...
  44. PREFOLDING AND PROTEIN FOLDING
    NICHOLAS COWAN; Fiscal Year: 2002
    ..We will coexpress the two prefoldin subunit homologs from the archaeon Methanococcus jannaschii in E...
  45. PREFOLDING AND PROTEIN FOLDING
    NICHOLAS COWAN; Fiscal Year: 1999
    ..We will coexpress the two prefoldin subunit homologs from the archaeon Methanococcus jannaschii in E...
  46. PREFOLDING AND PROTEIN FOLDING
    NICHOLAS COWAN; Fiscal Year: 2001
    ..We will coexpress the two prefoldin subunit homologs from the archaeon Methanococcus jannaschii in E...
  47. Structural Studies of the Pyrimidine Pathway
    Jacqueline Vitali; Fiscal Year: 2004
    ..this proposal we focus on the enzyme aspartate transcarbamoylase from the thermophilic and barophilic archaeon Methanococcus jannaschii...
  48. Maintenance of the tRNA 3' end
    Ya Ming Hou; Fiscal Year: 2007
    ..Because of the importance of CCA in maintenance of cellular life, the proposed studies will have a strong impact on human health and diseases. ..
  49. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 2003
    ..coli CysRS for its ability to distinguish cysteine from serine. These studies shall shed new light on the molecular interactions responsible for the accurate translation of the genetic code. ..
  50. Maintenance of the tRNA 3' end
    Ya Ming Hou; Fiscal Year: 2005
    ..Because of the importance of CCA in maintenance of cellular life, the proposed studies will have a strong impact on human health and diseases. ..
  51. FUNCTIONS OF tRNA MODIFICATIONS
    Ya Ming Hou; Fiscal Year: 2009
    ..abstract_text> ..
  52. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 2004
    ..coli CysRS for its ability to distinguish cysteine from serine. These studies shall shed new light on the molecular interactions responsible for the accurate translation of the genetic code. ..
  53. Transcriptional Nitrogen Regulation in Methanococcus
    JOHN LEIGH; Fiscal Year: 2006
    b>Methanococcus maripaludis is an ideal model organism for the study of transcriptional regulation in the Archaea. Through the study of a nitrogen assimilation regulon, novel aspects of transcriptional regulation are coming to light...
  54. GENOME SEQUENCE AND REGULATION IN M MARIPALUDIS
    JOHN LEIGH; Fiscal Year: 2000
    ..we propose to carry out three-fold (3X) random DNA sequencing of the genome of the methanogenic archaeon Methanococcus maripaludis...
  55. Activation of PI Specific Phospholipase C Enzymes
    MARY FEDARKO ROBERTS; Fiscal Year: 2010
    ..The studies of the bacterial enzymes may provide new treatments for slowing/inhibiting infections. ..
  56. ACTIVATION OF PI-SPECIFIC PHOSPHOLIPASE C ENZYMES
    Mary Roberts; Fiscal Year: 2005
    ..monocytogenes, an intracellular pathogen of humans that can cause serious infections in immunocompromised individuals, the work should also provide insight into how the enzyme aids in escape of the bacterium from the phagosome. ..
  57. GENOME SEQUENCE AND REGULATION IN M MARIPALUDIS
    JOHN LEIGH; Fiscal Year: 2002
    ..we propose to carry out three-fold (3X) random DNA sequencing of the genome of the methanogenic archaeon Methanococcus maripaludis...
  58. ACTIVATION OF PI-SPECIFIC PHOSPHOLIPASE C ENZYMES
    Mary Roberts; Fiscal Year: 2003
    ..This information will be critical in finding ways to manipulate these enzymes in vivo. ..
  59. ACTIVATION OF PI-SPECIFIC PHOSPHOLIPASE C ENZYMES
    Mary Roberts; Fiscal Year: 2001
    ..This information will be critical in finding ways to manipulate these enzymes in vivo. ..
  60. INTERFACIAL ACTIVATION OF SOLUBLE LIPOLYTIC ENZYMES
    Mary Roberts; Fiscal Year: 1990
    ..The latter play key roles in cellular activation including the production of inositol phosphates for intracellular Ca2+ mobilization and the generation of diglyceride for activation of protein kinase C...
  61. Transcriptional Nitrogen Regulation in Methanococcus
    JOHN LEIGH; Fiscal Year: 2004
    b>Methanococcus maripaludis is an ideal model organism for the study of transcriptional regulation in the Archaea. Through the study of a nitrogen assimilation regulon, novel aspects of transcriptional regulation are coming to light...
  62. INTERFACIAL ACTIVATION OF WATER SOLUBLE PHOSPHOLIPASES
    Mary Roberts; Fiscal Year: 2001
    ..abstract_text> ..
  63. NIF GENE REGULATION IN METHANOCOCCUS
    JOHN LEIGH; Fiscal Year: 1999
    ..The regulation of nif (nitrogen fixation) gene expression in the archaeon Methanococcus maripaludis will be investigated...
  64. Methanococcus maripaludis as an emerging model organism
    JOHN LEIGH; Fiscal Year: 2008
    b>Methanococcus maripaludis is emerging as a premier model for the methanogens and the Archaea. It grows unusually well, has an extensive set of facile genetic tools, and has a known complete annotated genome sequence...
  65. Methanococcus maripaludis as an emerging model organism
    JOHN LEIGH; Fiscal Year: 2007
    b>Methanococcus maripaludis is emerging as a premier model for the methanogens and the Archaea. It grows unusually well, has an extensive set of facile genetic tools, and has a known complete annotated genome sequence...
  66. GENOME SEQUENCE AND REGULATION IN M MARIPALUDIS
    JOHN LEIGH; Fiscal Year: 2001
    ..we propose to carry out three-fold (3X) random DNA sequencing of the genome of the methanogenic archaeon Methanococcus maripaludis...
  67. NIF GENE REGULATION IN METHANOCOCCUS
    JOHN LEIGH; Fiscal Year: 2000
    ..The regulation of nif (nitrogen fixation) gene expression in the archaeon Methanococcus maripaludis will be investigated...
  68. GENOME SEQUENCE AND REGULATION IN M MARIPALUDIS
    JOHN LEIGH; Fiscal Year: 2001
    ..we propose to carry out three-fold (3X) random DNA sequencing of the genome of the methanogenic archaeon Methanococcus maripaludis...
  69. Transcriptional Nitrogen Regulation in Methanococcus
    JOHN LEIGH; Fiscal Year: 2005
    b>Methanococcus maripaludis is an ideal model organism for the study of transcriptional regulation in the Archaea. Through the study of a nitrogen assimilation regulon, novel aspects of transcriptional regulation are coming to light...
  70. INTERFACIAL ACTIVATION OF WATER SOLUBLE PHOSPHOLIPASES
    Mary Roberts; Fiscal Year: 2003
    ..abstract_text> ..
  71. ACTIVATION OF PI-SPECIFIC PHOSPHOLIPASE C ENZYMES
    Mary Roberts; Fiscal Year: 2006
    ..monocytogenes, an intracellular pathogen of humans that can cause serious infections in immunocompromised individuals, the work should also provide insight into how the enzyme aids in escape of the bacterium from the phagosome. ..
  72. ACTIVATION OF PI-SPECIFIC PHOSPHOLIPASE C ENZYMES
    Mary Roberts; Fiscal Year: 2008
    ..monocytogenes, an intracellular pathogen of humans that can cause serious infections in immunocompromised individuals, the work should also provide insight into how the enzyme aids in escape of the bacterium from the phagosome. ..
  73. ACTIVATION OF PI-SPECIFIC PHOSPHOLIPASE C ENZYMES
    Mary Roberts; Fiscal Year: 2002
    ..This information will be critical in finding ways to manipulate these enzymes in vivo. ..
  74. NIF GENE REGULATION IN METHANOCOCCUS
    JOHN LEIGH; Fiscal Year: 2001
    ..The regulation of nif (nitrogen fixation) gene expression in the archaeon Methanococcus maripaludis will be investigated...