acidianus

Summary

Summary: A genus of facultatively anaerobic coccoid ARCHAEA, in the family SULFOLOBACEAE. Cells are highly irregular in shape and thermoacidophilic. Lithotrophic growth occurs aerobically via sulfur oxidation in some species. Distribution includes solfataric springs and fields, mudholes, and geothermically heated acidic marine environments.

Top Publications

  1. ncbi Purification and properties of the sulfur oxygenase/reductase from the acidothermophilic archaeon, Acidianus strain S5
    Cui Wei Sun
    Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing, P R China
    Extremophiles 7:131-4. 2003
  2. doi Novel archaeal plasmid pAH1 and its interactions with the lipothrixvirus AFV1
    Tamara Basta
    Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
    Mol Microbiol 71:23-34. 2009
  3. pmc Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles
    Xiao Yan You
    State Key Laboratory of Microbial Resources and Center for Environmental Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Bei Chen Xi Lu No Chao Yang District, Beijing, People s Republic of China
    Extremophiles 15:487-97. 2011
  4. pmc ORF157 from the archaeal virus Acidianus filamentous virus 1 defines a new class of nuclease
    Adeline Goulet
    Architecture et Fonction des Macromolcéules Biologiques, CNRS and Universités d Aix Marseille I and II, Marseille, France
    J Virol 84:5025-31. 2010
  5. pmc Chaperone role for proteins p618 and p892 in the extracellular tail development of Acidianus two-tailed virus
    Urte Scheele
    Archaea Centre, Department of Biology, University of Copenhagen, DK 2200 Copenhagen N, Denmark
    J Virol 85:4812-21. 2011
  6. doi Getting the best out of long-wavelength X-rays: de novo chlorine/sulfur SAD phasing of a structural protein from ATV
    Adeline Goulet
    Architecture et Fonction des Macromolécules Biologiques UMR 6098, CNRS, Universités d Aix Marseille I et II, Case 932, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
    Acta Crystallogr D Biol Crystallogr 66:304-8. 2010
  7. pmc Structure of the acidianus filamentous virus 3 and comparative genomics of related archaeal lipothrixviruses
    Gisle Vestergaard
    Centre for Comparative Genomics and Danish Archaea Centre, Department of Molecular Biology, Biocenter, Copenhagen University, Ole Maaløes Vej 5, DK 2200 Copenhagen N, Denmark
    J Virol 82:371-81. 2008
  8. pmc Viral diversity in hot springs of Pozzuoli, Italy, and characterization of a unique archaeal virus, Acidianus bottle-shaped virus, from a new family, the Ampullaviridae
    Monika Häring
    Molecular Biology of the Gene in Extremophiles Unit, Institut Pasteur, Paris, France
    J Virol 79:9904-11. 2005
  9. ncbi Genome of the Acidianus bottle-shaped virus and insights into the replication and packaging mechanisms
    Xu Peng
    Centre for Comparative Genomics, Department of Molecular Biology, Biocenter, Copenhagen University, Ole Maaløes Vej 5, DK 2200 Copenhagen N, Denmark
    Virology 364:237-43. 2007
  10. ncbi Biogenic scorodite crystallization by Acidianus sulfidivorans for arsenic removal
    Paula Gonzalez-Contreras
    Subdepartment of Environmental Technology, Wageningen University, 6700 EV Wageningen, The Netherlands
    Environ Sci Technol 44:675-80. 2010

Research Grants

  1. X-RAY SPECTROSCOPIC STUDIES ON HEME-COPPER OXIDASES
    Ninian Blackburn; Fiscal Year: 2001
  2. X-RAY SPECTROSCOPIC STUDIES ON HEME-COPPER OXIDASES
    Ninian Blackburn; Fiscal Year: 2002
  3. X-RAY SPECTROSCOPIC STUDIES ON HEME-COPPER OXIDASES
    Ninian Blackburn; Fiscal Year: 2003
  4. X-RAY SPECTROSCOPIC STUDIES ON HEME-COPPER OXIDASES
    Ninian Blackburn; Fiscal Year: 2004
  5. X-RAY SPECTROSCOPIC STUDIES ON HEME-COPPER OXIDASES
    Ninian Blackburn; Fiscal Year: 2006

Scientific Experts

  • Arnulf Kletzin
  • Aloysio Janner
  • Paula Gonzalez-Contreras
  • Norio Kurosawa
  • L Stout
  • Carlos Frazao
  • Xu Peng
  • Päivi H M Kinnunen
  • Ralf G Moll
  • Urte Scheele
  • W G Purschke
  • Magnus Lundgren
  • P Wittung-Stafshede
  • P Hellwig
  • David Prangishvili
  • Jason J Plumb
  • Jenny Keller
  • Ninian Blackburn
  • Claudio M Gomes
  • Tiago M Bandeiras
  • Roger A Garrett
  • Adeline Goulet
  • Tim Urich
  • Gisle Vestergaard
  • Sónia S Leal
  • Miguel Teixeira
  • Andreas Veith
  • Reinhard Rachel
  • Monika Häring
  • C M Gomes
  • Wei Zhu
  • Li Wang
  • Christian Cambillau
  • Valerie Campanacci
  • Tamara Basta
  • M Teixeira
  • José A Brito
  • Manuela M Pereira
  • Deirdre Mikkelsen
  • Kai Zhang
  • Fei Sun
  • Hugo M Botelho
  • Patrick Forterre
  • Ariane Bize
  • Christian Bauer
  • Smilja Todorovic
  • Shuang Jiang Liu
  • Abhilasha Bharadwaj
  • Zhiguo He
  • Zhen Yuan Nie
  • Yi Yang
  • Jin Lan Xia
  • Guan Zhou Qiu
  • An An Peng
  • Zhiyang Dong
  • Xiaoyun Pang
  • Zhi Wei Chen
  • Xiao Yan You
  • Qihou Li
  • Courtney A Benson
  • Zheng Guo He
  • M Ishii
  • Marco Marcia
  • Yanwu Huo
  • Miguel Ortiz-Lombardia
  • Shiraz A Shah
  • Peter Redder
  • Herman van Tilbeurgh
  • Catarina Felisberto-Rodrigues
  • Nicolas Leulliot
  • Stéphanie Blangy
  • Manuela Baumgartner
  • Mei Li
  • Ana P Batista
  • Cheng Ying Jiang
  • Daniel H Murgida
  • Peter Hildebrandt
  • Kerstin Seyfarth
  • Qunxin She
  • Ulrike Kappler
  • Clemens Vonrhein
  • Alastair G McEwan
  • Lindsay I Sly
  • Margarida Archer
  • Stephan Bathe
  • Kim Brugger
  • Lanming Chen
  • Naoki Yoshida
  • Chun Qiang Liu
  • John P McDonald

Detail Information

Publications100

  1. ncbi Purification and properties of the sulfur oxygenase/reductase from the acidothermophilic archaeon, Acidianus strain S5
    Cui Wei Sun
    Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing, P R China
    Extremophiles 7:131-4. 2003
    The sulfur oxygenase/reductase (SOR) of Acidianus strain S5 was purified and characterized after expressing the SOR gene in a recombinant strain of Escherichia coli...
  2. doi Novel archaeal plasmid pAH1 and its interactions with the lipothrixvirus AFV1
    Tamara Basta
    Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
    Mol Microbiol 71:23-34. 2009
    ..Here we describe an Acidianus strain which carries naturally a novel 28 kb conjugative plasmid-like element, pAH1, and also serves as a ..
  3. pmc Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles
    Xiao Yan You
    State Key Laboratory of Microbial Resources and Center for Environmental Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Bei Chen Xi Lu No Chao Yang District, Beijing, People s Republic of China
    Extremophiles 15:487-97. 2011
    The Acidianus hospitalis W1 genome consists of a minimally sized chromosome of about 2.13 Mb and a conjugative plasmid pAH1 and it is a host for the model filamentous lipothrixvirus AFV1...
  4. pmc ORF157 from the archaeal virus Acidianus filamentous virus 1 defines a new class of nuclease
    Adeline Goulet
    Architecture et Fonction des Macromolcéules Biologiques, CNRS and Universités d Aix Marseille I and II, Marseille, France
    J Virol 84:5025-31. 2010
    b>Acidianus filamentous virus 1 (AFV1) (Lipothrixviridae) is an enveloped filamentous virus that was characterized from a crenarchaeal host...
  5. pmc Chaperone role for proteins p618 and p892 in the extracellular tail development of Acidianus two-tailed virus
    Urte Scheele
    Archaea Centre, Department of Biology, University of Copenhagen, DK 2200 Copenhagen N, Denmark
    J Virol 85:4812-21. 2011
    The crenarchaeal Acidianus two-tailed virus (ATV) undergoes a remarkable morphological development, extracellularly and independently of host cells, by growing long tails at each end of a spindle-shaped virus particle...
  6. doi Getting the best out of long-wavelength X-rays: de novo chlorine/sulfur SAD phasing of a structural protein from ATV
    Adeline Goulet
    Architecture et Fonction des Macromolécules Biologiques UMR 6098, CNRS, Universités d Aix Marseille I et II, Case 932, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
    Acta Crystallogr D Biol Crystallogr 66:304-8. 2010
    The structure of a 14 kDa structural protein from Acidianus two-tailed virus (ATV) was solved by single-wavelength anomalous diffraction (SAD) phasing using X-ray data collected at 2.0 A wavelength...
  7. pmc Structure of the acidianus filamentous virus 3 and comparative genomics of related archaeal lipothrixviruses
    Gisle Vestergaard
    Centre for Comparative Genomics and Danish Archaea Centre, Department of Molecular Biology, Biocenter, Copenhagen University, Ole Maaløes Vej 5, DK 2200 Copenhagen N, Denmark
    J Virol 82:371-81. 2008
    Four novel filamentous viruses with double-stranded DNA genomes, namely, Acidianus filamentous virus 3 (AFV3), AFV6, AFV7, and AFV8, have been characterized from the hyperthermophilic archaeal genus Acidianus, and they are assigned to ..
  8. pmc Viral diversity in hot springs of Pozzuoli, Italy, and characterization of a unique archaeal virus, Acidianus bottle-shaped virus, from a new family, the Ampullaviridae
    Monika Häring
    Molecular Biology of the Gene in Extremophiles Unit, Institut Pasteur, Paris, France
    J Virol 79:9904-11. 2005
    ..to represent viable virions of five novel viruses which infect members of the hyperthermophilic archaeal genus Acidianus. One of these, named the Acidianus bottle-shaped virus, ABV, exhibits a previously unreported morphotype...
  9. ncbi Genome of the Acidianus bottle-shaped virus and insights into the replication and packaging mechanisms
    Xu Peng
    Centre for Comparative Genomics, Department of Molecular Biology, Biocenter, Copenhagen University, Ole Maaløes Vej 5, DK 2200 Copenhagen N, Denmark
    Virology 364:237-43. 2007
    The Acidianus bottle-shaped virus, ABV, infects strains of the hyperthermophilic archaeal genus Acidianus and is morphologically distinct from all other known viruses...
  10. ncbi Biogenic scorodite crystallization by Acidianus sulfidivorans for arsenic removal
    Paula Gonzalez-Contreras
    Subdepartment of Environmental Technology, Wageningen University, 6700 EV Wageningen, The Netherlands
    Environ Sci Technol 44:675-80. 2010
    ..In the present study we show that the thermoacidophilic iron-oxidizing archaeon Acidianus sulfidivorans is able to precipitate scorodite in the absence of any primary minerals or seed crystals, when ..
  11. pmc The thermo- and acido-stable ORF-99 from the archaeal virus AFV1
    Adeline Goulet
    Architecture et Fonction des Macromolecules Biologiques, CNRS and Universités d Aix Marseille I and II, 163 Avenue de Luminy, Marseille Cedex 9, France
    Protein Sci 18:1316-20. 2009
    b>Acidianus Filamentous Virus 1 (AFV1), isolated from acidic hot springs, is an enveloped lipid-containing archaeal filamentous virus with a linear double-stranded DNA genome...
  12. pmc Crystal structure of AFV1-102, a protein from the acidianus filamentous virus 1
    Jenny Keller
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Universite Paris Sud, IFR115, UMR8619 CNRS, 91405 Orsay, France
    Protein Sci 18:845-9. 2009
    ..project on the proteins of these viruses, we present here the structure of a 102 amino acid protein from acidianus filamentous virus 1 (AFV1-102)...
  13. ncbi AFV1, a novel virus infecting hyperthermophilic archaea of the genus acidianus
    Marcus Bettstetter
    Department of Microbiology, Archaea Centre, University of Regensburg, Universitatsstrasse 31, D 93053 Regensburg, Germany
    Virology 315:68-79. 2003
    We describe a novel virus, AFV1, of the hyperthermophilic archaeal genus Acidianus. Filamentous virions are covered with a lipid envelope and contain at least five different proteins with molecular masses in the range of 23-130 kDa and a ..
  14. ncbi Structural and genomic properties of the hyperthermophilic archaeal virus ATV with an extracellular stage of the reproductive cycle
    David Prangishvili
    Molecular Biology of the Gene in Extremophiles Unit, Institut Pasteur, Paris, France
    J Mol Biol 359:1203-16. 2006
    A novel virus, ATV, of the hyperthermophilic archaeal genus Acidianus has the unique property of undergoing a major morphological development outside of, and independently of, the host cell...
  15. doi The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc(1) complexes
    Tiago M Bandeiras
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Av da Republica EAN, 2780 157 Oeiras, Portugal
    Biochim Biophys Acta 1787:37-45. 2009
    A novel cytochrome ba complex was isolated from aerobically grown cells of the thermoacidophilic archaeon Acidianus ambivalens. The complex was purified with two subunits, which are encoded by the cbsA and soxN genes...
  16. pmc The sulphur oxygenase reductase from Acidianus ambivalens is a multimeric protein containing a low-potential mononuclear non-haem iron centre
    Tim Urich
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Biochem J 381:137-46. 2004
    The SOR (sulphur oxygenase reductase) is the initial enzyme in the sulphur-oxidation pathway of Acidianus ambivalens...
  17. doi Crystal structure studies on sulfur oxygenase reductase from Acidianus tengchongensis
    Mei Li
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, PR China
    Biochem Biophys Res Commun 369:919-23. 2008
    ..In this study, crystal structures of wild type and mutants of SOR from Acidianus tengchongensis (SOR-AT) in two different crystal forms were determined and it was observed that 24 identical SOR ..
  18. ncbi Acidianus sulfidivorans sp. nov., an extremely acidophilic, thermophilic archaeon isolated from a solfatara on Lihir Island, Papua New Guinea, and emendation of the genus description
    Jason J Plumb
    Centre for Environment and Life Sciences, CSIRO Land and Water, Private Bag No 5, Wembley, Western Australia 6913, Australia
    Int J Syst Evol Microbiol 57:1418-23. 2007
    ..strain JP7(T) was shown to belong to the Sulfolobaceae, being most closely related to the type strains of Acidianus ambivalens (93.7 %) and Acidianus infernus (93.6 %)...
  19. pmc Acidianus filamentous virus 1 coat proteins display a helical fold spanning the filamentous archaeal viruses lineage
    Adeline Goulet
    Architecture et Fonction des Macromolecules Biologiques, Centre national de la Recherche Scientifique and Universités Aix Marseille I and II, Architecture et Fonction des Macromolecules Biologiques, Unite Mixte de Recherche 6098, Case 932, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
    Proc Natl Acad Sci U S A 106:21155-60. 2009
    b>Acidianus filamentous virus 1 (AFV1), a member of the Lipothrixviridae family, infects the hyperthermophilic, acidophilic crenarchaeaon Acidianus hospitalis...
  20. ncbi Acidianus manzaensis sp. nov., a novel thermoacidophilic archaeon growing autotrophically by the oxidation of H2 with the reduction of Fe3+
    Naoki Yoshida
    Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, 113 8657, Japan
    Curr Microbiol 53:406-11. 2006
    ..9 mol%. From 16S rDNA analysis, it was determined that strain NA-1 is closely related to Acidianus ambivalens (93.1%) and Acidianus infernus (93.0%)...
  21. pmc Key role of cysteine residues in catalysis and subcellular localization of sulfur oxygenase-reductase of Acidianus tengchongensis
    Zhi Wei Chen
    Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People s Republic of China
    Appl Environ Microbiol 71:621-8. 2005
    ..cysteine residues within two conserved motifs (V-G-P-K-V-C(31) and C(101)-X-X-C(104); numbering according to the Acidianus tengchongensis numbering system)...
  22. ncbi The sulfur oxygenase reductase from Acidianus ambivalens is an icosatetramer as shown by crystallization and Patterson analysis
    Tim Urich
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Biochim Biophys Acta 1747:267-70. 2005
    ..initial enzyme in the aerobic sulfur metabolism of the thermoacidophilic and chemolithoautotrophic crenarchaeote Acidianus ambivalens...
  23. doi Distinct symmetry and limited peptide refolding activity of the thermosomes from the acidothermophilic archaea Acidianus tengchongensis S5(T)
    Li Wang
    Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China
    Biochem Biophys Res Commun 393:228-34. 2010
    Recombinant thermosomes from the Acidianus tengchongensis strain S5(T) were purified to homogeneity and assembled in vitro into homo-oligomers (rATcpnalpha or rATcpnbeta) and hetero-oligomers (rATcpnalphabeta)...
  24. ncbi Virology: independent virus development outside a host
    Monika Häring
    Molecular Biology of the Gene in Extremophiles Unit, Institut Pasteur, 75724 Paris Cedex 15, France
    Nature 436:1101-2. 2005
    ..This host-independent morphological development may be a strategy for viral survival in an environment that is unusually harsh and has limited host availability...
  25. ncbi Identification of core active site residues of the sulfur oxygenase reductase from Acidianus ambivalens by site-directed mutagenesis
    Tim Urich
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    FEMS Microbiol Lett 248:171-6. 2005
    The sulfur oxygenase reductase (SOR) is the initial enzyme in the sulfur oxidation pathway of Acidianus ambivalens. The SOR is composed of 308 aa residues, three of which are cysteines, and contains a mononuclear non-heme iron site...
  26. ncbi Membrane-bound hydrogenase and sulfur reductase of the hyperthermophilic and acidophilic archaeon Acidianus ambivalens
    Simone Laska
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Microbiology 149:2357-71. 2003
    ..were purified from solubilized membrane fractions of anaerobically grown cells of the sulfur-dependent archaeon Acidianus ambivalens and the corresponding genes were sequenced...
  27. ncbi Prokaryotic sulfur oxidation
    Cornelius G Friedrich
    Department of Biochemical and Chemical Engineering, University of Dortmund, D 44221 Dortmund, Germany
    Curr Opin Microbiol 8:253-9. 2005
    ..This indicates a different pathway for oxidation of sulfur to sulfate. Acidophilic bacteria oxidize sulfur by a system different from the Sox enzyme system, as do chemotrophic endosymbiotic bacteria...
  28. pmc Theoretical identification of proton channels in the quinol oxidase aa3 from Acidianus ambivalens
    Bruno L Victor
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, 2781 901 Oeiras, Portugal
    Biophys J 87:4316-25. 2004
    ..In this work we modeled the structure of the quinol oxidase from Acidianus ambivalens using comparative modeling techniques for identifying proton channels...
  29. ncbi Comparative architecture of octahedral protein cages. II. Interplay between structural elements
    Aloysio Janner
    Theoretical Physics, Radboud University, Toernooiveld, NL 6525 ED Nijmegen, The Netherlands
    Acta Crystallogr A 64:503-12. 2008
    ....
  30. ncbi Structure and coordination of CuB in the Acidianus ambivalens aa3 quinol oxidase heme-copper center
    Tiago M Bandeiras
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Av da Republica EAN, 2784 505, Oeiras, Portugal
    J Biol Inorg Chem 10:625-35. 2005
    The coordination environment of the Cu(B) center of the quinol oxidase from Acidianus ambivalens, a type B heme-copper oxygen reductase, was investigated by Fourier transform (FT) IR and extended X-ray absorption fine structure (EXAFS) ..
  31. ncbi Comparative architecture of octahedral protein cages. I. Indexed enclosing forms
    Aloysio Janner
    Theoretical Physics, Radboud University, Toernooiveld, NL 6525 ED Nijmegen, The Netherlands
    Acta Crystallogr A 64:494-502. 2008
    ..The determination of the cubic indices of the monomeric vertices opens the possibility of the way back analysis, bottom-to-top, as discussed in paper II [Janner (2008). Acta Cryst. A64, 503-512]...
  32. ncbi A spectroscopic study of the temperature induced modifications on ferredoxin folding and iron-sulfur moieties
    Smilja Todorovic
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Av Republica EAN, 2781 901 Oeiras, Portugal
    Biochemistry 46:10733-8. 2007
    Thermal perturbation of the dicluster ferredoxin from Acidianus ambivalens was investigated employing a toolbox of spectroscopic methods...
  33. pmc Presence of acetyl coenzyme A (CoA) carboxylase and propionyl-CoA carboxylase in autotrophic Crenarchaeota and indication for operation of a 3-hydroxypropionate cycle in autotrophic carbon fixation
    C Menendez
    Mikrobiologie, Institut Biologie II, Universitat Freiburg, Freiburg, Germany
    J Bacteriol 181:1088-98. 1999
    ..aurantiacus. The investigation was extended to the autotrophic archaea Sulfolobus metallicus and Acidianus infernus, which showed acetyl-CoA and propionyl-CoA carboxylase activities in extracts of autotrophically grown ..
  34. ncbi Remarkable morphological diversity of viruses and virus-like particles in hot terrestrial environments
    R Rachel
    Lehrstuhl fur Mikrobiologie und Archaeenzentrum, Universitat Regensburg, Germany
    Arch Virol 147:2419-29. 2002
    ..Viral hosts in the acidic spring were members of the hyperthermophilic archaeal genus Acidianus.
  35. ncbi Estimation of genome sizes of hyperthermophiles
    C Baumann
    Lehrstuhl fur Mikrobiologie und Archaeenzentrum, Universitat Regensburg, Germany
    Extremophiles 2:101-8. 1998
    ..The Metallosphaera-Acidianus group, plus Sulfolobus metallicus, have genomes of ca. 1...
  36. ncbi The hyper-thermostable Fe-superoxide dismutase from the Archaeon Acidianus ambivalens: characterization, recombinant expression, crystallization and effects of metal exchange
    S Kardinahl
    Institute of Biochemistry, Medical University of Lubeck, Germany
    Biol Chem 381:1089-101. 2000
    An iron-containing superoxide dismutase (SOD; EC 1.15.1.1) of the hyperthermophilic archaeon Acidianus ambivalens (Aa-SOD) has been purified and characterized and the gene has been cloned and sequenced...
  37. pmc Protein-protein, protein-RNA and protein-lipid interactions of signal-recognition particle components in the hyperthermoacidophilic archaeon Acidianus ambivalens
    Ralf G Moll
    Department of Biochemistry, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    Biochem J 374:247-54. 2003
    The signal-recognition particle (SRP) of one of the most acidophilic and hyperthermophilic archaeal cells, Acidianus ambivalens, and its putative receptor component, FtsY (prokaryotic SRP receptor), were investigated in detail. A...
  38. pmc Crystal structure of AFV3-109, a highly conserved protein from crenarchaeal viruses
    Jenny Keller
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, CNRS UMR 8619, Université Paris 11, IFR115, Batiment 430, 91405 Orsay, France
    Virol J 4:12. 2007
    ..is shared by overlapping subsets of these viruses, and the most conserved gene, exemplified by the ORF109 of the Acidianus Filamentous Virus 3, AFV3, is present on genomes of members of three viral familes, the Lipothrixviridae, ..
  39. doi Microbial diversity of boron-rich volcanic hot springs of St. Lucia, Lesser Antilles
    Lisa M Stout
    Department of Geology and Geophysics, Yale University, New Haven, CT 06520, USA
    FEMS Microbiol Ecol 70:402-12. 2009
    ..The archaeal component of all pools was almost exclusively Acidianus spp. and did not vary between sites with different chemical characteristics...
  40. ncbi FTIR spectroscopic characterization of the cytochrome aa3 from Acidianus ambivalens: evidence for the involvement of acidic residues in redox coupled proton translocation
    Petra Hellwig
    Institut fur Biophysik, Universitat Frankfurt, Theodor Stern Kai 7 Haus 75, Germany
    Biochemistry 42:6179-84. 2003
    The aa(3)-type quinol oxidase from Acidianus ambivalens is a divergent member of the heme-copper oxidases superfamily, namely, concerning the putative channels for intraprotein proton conduction...
  41. ncbi Role of a novel disulfide bridge within the all-beta fold of soluble Rieske proteins
    Hugo M Botelho
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    J Biol Inorg Chem 15:271-81. 2010
    ..We establish that in the Acidianus ambivalens representative, Rieske ferredoxin 2 (RFd2), these cysteines form a novel disulfide bond within the ..
  42. ncbi Acidianus ambivalens type-II NADH dehydrogenase: genetic characterisation and identification of the flavin moiety as FMN
    Tiago M Bandeiras
    Instituto de Tecnologia Qui mica e Biológica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Oeiras, Portugal
    FEBS Lett 531:273-7. 2002
    The thermoacidophilic archaeon Acidianus ambivalens contains a monomeric 47 kDa type-II NADH dehydrogenase (NDH), which contains a covalently bound flavin...
  43. doi A new structure-based classification of sulfide:quinone oxidoreductases
    Marco Marcia
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, 60596 Frankfurt am Main, Germany
    Proteins 78:1073-83. 2010
    ..Proc Natl Acad Sci USA 2009; 106:9625-9630) and the archaeon Acidianus ambivalens (Brito et al...
  44. ncbi Acidianus ambivalens Complex II typifies a novel family of succinate dehydrogenases
    R S Lemos
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apt 127, Oeiras, 2780 156, Portugal
    Biochem Biophys Res Commun 281:141-50. 2001
    Complex II from the thermoacidophilic archaeon Acidianus ambivalens, an archetype of an emerging class of succinate dehydrogenases (SDH), was extracted from intact membranes and purified to homogeneity...
  45. ncbi Archaeal diversity in two thermophilic chalcopyrite bioleaching reactors
    Deirdre Mikkelsen
    Centre for Bacterial Diversity and Identification, School of Molecular and Microbial Sciences, University of Queensland, Brisbance, Australia
    Environ Microbiol 8:2050-6. 2006
    ..The remaining clones were affiliated with Stygiolobus azoricus (11%), Metallosphaera sp. J1 (8%), Acidianus infernus (2%), and a novel phylotype related to Sulfurisphaera ohwakuensis (10%)...
  46. ncbi Cloning and heterologous expression of a sulfur oxygenase/reductase gene from the thermoacidophilic archaeon Acidianus sp. S5 in Escherichia coli
    Z He
    State Key Laboratory of Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing, PR China
    FEMS Microbiol Lett 193:217-21. 2000
    A thermoacidophilic, obligately chemolithotrophic, facultatively aerobic archaebacterium, Acidianus sp. S5, was isolated from acidothermal springs in southwest China. The sulfur oxygenase/reductase (SOR) gene of Acidianus sp...
  47. ncbi Functional properties of the quinol oxidase from Acidianus ambivalens and the possible catalytic role of its electron donor--studies on the membrane-integrated and purified enzyme
    A Giuffre
    Department of Biochemical Sciences and CNR Center of Molecular Biology, University of Rome La Sapienza, Italy
    Eur J Biochem 250:383-8. 1997
    The aa3 quinol oxidase has been purified from the thermoacidophilic archaea Acidianus ambivalens as a three-redox-centers enzyme. The functional properties of this oxidase both as purified and in its most integral form (i.e...
  48. ncbi Dynamics of the binuclear center of the quinol oxidase from Acidianus ambivalens
    A Aagaard
    Department of Biochemistry and Biophysics, Goteborg University, Sweden
    Biochemistry 38:10032-41. 1999
    ..monoxide binding to the fully reduced quinol oxidase (cytochrome aa(3)) from the hyperthermophilic archaeon Acidianus ambivalens...
  49. pmc Molecular analysis of pDL10 from Acidianus ambivalens reveals a family of related plasmids from extremely thermophilic and acidophilic archaea
    A Kletzin
    Institute of Microbiology and Genetics, Darmstadt University of Technology, 64287 Darmstadt, Germany
    Genetics 152:1307-14. 1999
    The 7598-bp plasmid pDL10 from the extremely thermophilic, acidophilic, and chemolithoautotrophic Archaeon Acidianus ambivalens was sequenced. It contains 10 open reading frames (ORFs) organized in five putative operons...
  50. ncbi The NADH oxidase from the thermoacidophilic archaea Acidianus ambivalens: isolation and physicochemical characterisation
    C M Gomes
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    Biochem Biophys Res Commun 243:412-5. 1998
    ..activity (NADH: acceptor oxidoreductase) was isolated from the soluble fraction of the thermoacidophilic archaea Acidianus ambivalens. The protein is a monomer with a molecular mass of 70 kDa and contains FAD as single cofactor...
  51. ncbi Ambineela, an unusual blue protein isolated from the archaeon Acidianus ambivalens
    C M Gomes
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    Biochem Biophys Res Commun 249:23-5. 1998
    A novel blue protein, named ambineela, was isolated from the soluble extract of the thermoacidophilic archaeon Acidianus ambivalens. In solution, the purified protein is a monomer with 50 kDa and has a basic character (pI approximately 8...
  52. pmc Structure and genome organization of AFV2, a novel archaeal lipothrixvirus with unusual terminal and core structures
    Monika Häring
    Unite de Biologie Moleculaire du Gene chez les Extremophiles, Institut Pasteur, rue Dr Roux 25, 75724 Paris Cedex 15, France
    J Bacteriol 187:3855-8. 2005
    A novel filamentous virus, AFV2, from the hyperthermophilic archaeal genus Acidianus shows structural similarity to lipothrixviruses but differs from them in its unusual terminal and core structures...
  53. ncbi The isolation and use of iron-oxidizing, moderately thermophilic acidophiles from the Collie coal mine for the generation of ferric iron leaching solution
    P H M Kinnunen
    Institute of Environmental Engineering and Biotechnology, Tampere University of Technology, PO Box 541, 33101 Tampere, Finland
    Appl Microbiol Biotechnol 60:748-53. 2003
    ..3 g Fe(2+) l(-1) x h(-1). These rates compare favourably with the iron-oxidation rate of Acidianus brierleyi in shake-flasks, but are considerably lower than mesophilic iron-oxidation rates.
  54. ncbi Crystallisation and preliminary structure determination of a NADH: quinone oxidoreductase from the extremophile Acidianus ambivalens
    José A Brito
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apartado 127, 2781 901 Oeiras, Portugal
    Biochim Biophys Acta 1764:842-5. 2006
    ..structure determination of a NDH-2 that contains a covalently bound FAD, isolated from the membrane fraction of Acidianus ambivalens, a hyperthermoacidophilic archaeon capable of growing at 80 degrees C and pH 2.0...
  55. doi Thermophilic archaeal community succession and function change associated with the leaching rate in bioleaching of chalcopyrite
    Wei Zhu
    School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China
    Bioresour Technol 133:405-13. 2013
    The community succession and function change of thermophilic archaea Acidianus brierleyi, Metallosphaera sedula, Acidianus manzaensis and Sulfolobus metallicus were studied by denaturing gradient gel electrophoresis (DGGE) analysis of ..
  56. ncbi Acidophiles of saline water at thermal vents of Vulcano, Italy
    Susan Simmons
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
    Extremophiles 6:201-7. 2002
    ..sequences that comprised a clone bank representing a high-temperature sample (75 degrees C) corresponded to those of Acidianus brierleyi and of thermophiles previously isolated from Vulcano, Thermoplasma volcanium and Acidianus infernus.
  57. pmc Characterization of a bifunctional archaeal acyl coenzyme A carboxylase
    Songkran Chuakrut
    Laboratory of Applied Microbiology, Department of Biotechnology, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8567, Japan
    J Bacteriol 185:938-47. 2003
    Acyl coenzyme A carboxylase (acyl-CoA carboxylase) was purified from Acidianus brierleyi...
  58. ncbi Bioleaching of spent hydrotreating catalyst by acidophilic thermophile Acidianus brierleyi: Leaching mechanism and effect of decoking
    Abhilasha Bharadwaj
    Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore
    Bioresour Technol 130:673-80. 2013
    Bioleaching of spent hydrotreating catalyst by thermophillic archae Acidianus brierleyi was investigated...
  59. doi The effect of culture condition and ionic strength on proton adsorption at the surface of the extreme thermophile Acidianus manzaensis
    Zhiguo He
    School of Minerals Processing and Bioengineering, Central South University, No 932, Lushan South Road, Changsha 410083, China
    Colloids Surf B Biointerfaces 102:667-73. 2013
    ..In this study, we characterize proton adsorption of the extreme thermophile Acidianus manzaensis by performing acid-base titrations and electrophoretic mobility measurements in NaNO(3) solution (0...
  60. ncbi Rapid specific detection and quantification of bacteria and archaea involved in mineral sulfide bioleaching using real-time PCR
    Chun Qiang Liu
    CSIRO Molecular and Health Technologies PO Box 184, North Ryde NSW 1670, Australia
    Biotechnol Bioeng 94:330-6. 2006
    ..designed and validated for specific detection and quantification of DNA isolated from representative strains of Acidianus brierleyi, Sulfolobus sp...
  61. pmc Flexible interwoven termini determine the thermal stability of thermosomes
    Kai Zhang
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Protein Cell 4:432-44. 2013
    ..Here, we selected the group II chaperonins (cpn-α and cpn-β), also called thermosomes, from Acidianus tengchongensis and investigated their assembly and thermal stability...
  62. ncbi An archaeal b-type cytochrome containing a nonfunctional carbonic anhydrase-like domain
    Claudio M Gomes
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apt 127, 2780 156 Oeiras, Portugal
    J Biol Inorg Chem 7:483-9. 2002
    A new type of cytochrome b was isolated from the cytoplasmatic fraction of the archaeon Acidianus ambivalens, which is the first soluble cytochrome found in this member of the thermoacidophilic order of the Sulfolobales...
  63. ncbi Studies on the degradation pathway of iron-sulfur centers during unfolding of a hyperstable ferredoxin: cluster dissociation, iron release and protein stability
    Sónia S Leal
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Av Republica, 2784 505, Oeiras, Portugal
    J Biol Inorg Chem 9:987-96. 2004
    The ferredoxin from the thermoacidophile Acidianus ambivalens is a representative of the archaeal family of di-cluster [3Fe-4S][4Fe-4S] ferredoxins...
  64. ncbi X-ray Structure of a self-compartmentalizing sulfur cycle metalloenzyme
    Tim Urich
    Darmstadt University of Technology, Institute of Microbiology and Genetics, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Science 311:996-1000. 2006
    ..7 angstrom-resolution structure of the sulfur oxygenase reductase from the thermoacidophilic archaeon Acidianus ambivalens, which catalyzes an oxygen-dependent disproportionation of elemental sulfur...
  65. pmc High stability of a ferredoxin from the hyperthermophilic archaeon A. ambivalens: involvement of electrostatic interactions and cofactors
    C Moczygemba
    Chemistry Department, Tulane University, New Orleans, Louisiana 70118, USA
    Protein Sci 10:1539-48. 2001
    The ferredoxin from the thermophilic archaeon Acidianus ambivalens is a small monomeric seven-iron protein with a thermal midpoint (T(m)) of 122 degrees C (pH 7)...
  66. ncbi A new type-II NADH dehydrogenase from the archaeon Acidianus ambivalens: characterization and in vitro reconstitution of the respiratory chain
    C M Gomes
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    J Bioenerg Biomembr 33:1-8. 2001
    A new type-II NADH dehydrogenase (NDH-II) was isolated from the hyperthermoacidophilic archaeon Acidianus ambivalens...
  67. ncbi Stability and folding of the ferredoxin from the hyperthermophilic archaeon Acidianus ambivalens
    P Wittung-Stafshede
    Chemistry Department, Tulane University, New Orleans, LA 70118, USA
    J Inorg Biochem 78:35-41. 2000
    The ferredoxin from the thermophilic archaeon Acidianus ambivalens is a small monomeric protein containing two iron-sulfur centres, one [3Fe-4S](1+/0) and one [4Fe-4S](2+/1+)...
  68. ncbi The unusual iron sulfur composition of the Acidianus ambivalens succinate dehydrogenase complex
    C M Gomes
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apt 127, 2780, Oeiras, Portugal
    Biochim Biophys Acta 1411:134-41. 1999
    The succinate dehydrogenase complex of the thermoacidophilic archaeon Acidianus ambivalens was investigated kinetically and by EPR spectroscopy in its most intact form, i.e., membrane bound...
  69. pmc Crystal structure of group II chaperonin in the open state
    Yanwu Huo
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Structure 18:1270-9. 2010
    ..Here, we report the first complete crystal structure of thermosome (rATcpnβ) in the open state from Acidianus tengchongensis...
  70. doi Sulfur oxidation activities of pure and mixed thermophiles and sulfur speciation in bioleaching of chalcopyrite
    Wei Zhu
    Key Lab of Biometallurgy of Ministry of Education of China, School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China
    Bioresour Technol 102:3877-82. 2011
    The sulfur oxidation activities of four pure thermophilic archaea Acidianus brierleyi (JCM 8954), Metallosphaera sedula (YN 23), Acidianus manzaensis (YN 25) and Sulfolobus metallicus (YN 24) and their mixture in bioleaching chalcopyrite ..
  71. doi Microbial diversity in nonsulfur, sulfur and iron geothermal steam vents
    Courtney A Benson
    Department of Biology, San Diego State University, San Diego, CA 92182 4614, USA
    FEMS Microbiol Ecol 76:74-88. 2011
    ..Analysis of archaeal 16S rRNA gene sequences showed that sulfur steam deposits were dominated by Sulfolobus and Acidianus, while nonsulfur deposits contained mainly unknown Crenarchaeota...
  72. ncbi Domain structure, GTP-hydrolyzing activity and 7S RNA binding of Acidianus ambivalens ffh-homologous protein suggest an SRP-like complex in archaea
    R Moll
    Institute of Biochemistry, Medical University of Lubeck, Germany
    Eur J Biochem 259:441-8. 1999
    ..The gene encoding the Ffh homologue in the hyperthermophilic archaeote Acidianus ambivalens has been cloned and sequenced. Recombinant Ffh protein was expressed in E...
  73. ncbi Tetramitus thermacidophilus n. sp., an amoeboflagellate from acidic hot springs
    Manuela Baumgartner
    Lehrstuhl fur Mikrobiologie, Universitat Regensburg, D 93053 Regensburg, Germany
    J Eukaryot Microbiol 56:201-6. 2009
    ..It was grown in monoxenic culture on the archaeon Acidianus brierleyi as food. Tetramitus thermacidophilus multiplies in a pH range from 1...
  74. ncbi Sulfurisphaera ohwakuensis gen. nov., sp. nov., a novel extremely thermophilic acidophile of the order Sulfolobales
    N Kurosawa
    Department of Bioengineering, Faculty of Engineering, Soka University, Tokyo, Japan
    Int J Syst Bacteriol 48:451-6. 1998
    ..The G + C content of DNA from TA-1T is 33 mol%. Although these properties resemble those of the genus Acidianus, the sequence of the 16S rRNA gene from strain TA-1T was more similar to that of species of Stygiolobus than of ..
  75. doi The community dynamics of major bioleaching microorganisms during chalcopyrite leaching under the effect of organics
    Qihou Li
    School of Metallurgical Science and Engineering, Central South University, Changsha, Hunan, China
    Curr Microbiol 63:164-72. 2011
    ..Similarly, bioleaching rate was enhanced under the effect of yeast extract at 50°C owing to the work of Acidianus brierleyi...
  76. pmc Substrate pathways and mechanisms of inhibition in the sulfur oxygenase reductase of acidianus ambivalens
    Andreas Veith
    Institute of Microbiology and Genetics, Technische Universität Darmstadt Darmstadt, Germany
    Front Microbiol 2:37. 2011
    ..reductase (SOR) is the initial enzyme of the sulfur oxidation pathway in the thermoacidophilic Archaeon Acidianus ambivalens. The SOR catalyzes an oxygen-dependent sulfur disproportionation to H(2)S, sulfite and thiosulfate...
  77. pmc The sulfur oxygenase reductase from the mesophilic bacterium Halothiobacillus neapolitanus is a highly active thermozyme
    Andreas Veith
    Institute of Microbiology and Genetics, Technische Universitat Darmstadt, Darmstadt, Germany
    J Bacteriol 194:677-85. 2012
    ..adopts secondary and quaternary structures similar to those of the 24-subunit enzyme from the hyperthermophile Acidianus ambivalens (AaSOR)...
  78. ncbi The signal recognition particle receptor alpha subunit of the hyperthermophilic archaeon Acidianus ambivalens exhibits an intrinsic GTP-hydrolyzing activity
    R Moll
    Institut fur Biochemie, Medizinische Universitat zu Lubeck
    Biochim Biophys Acta 1335:218-30. 1997
    Two adjacent genes of the acidophilic and hyperthermophilic crenarchaeon Acidianus ambivalens were cloned and sequenced. The 1.6 kb genomic nucleotide sequence under investigation consists of the 1...
  79. ncbi Autotrophic carbon dioxide fixation in Acidianus brierleyi
    M Ishii
    Department of Biotechnology, University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113, Japan
    Arch Microbiol 166:368-71. 1996
    The autotrophic CO2 fixation pathway in Acidianus brierleyi, a facultatively anaerobic thermoacidophilic archaebacterium, was investigated by measuring enzymatic activities from autotrophic, mixotrophic, and heterotrophic cultures...
  80. pmc The terminal quinol oxidase of the hyperthermophilic archaeon Acidianus ambivalens exhibits a novel subunit structure and gene organization
    W G Purschke
    Institute of Biochemistry, Medical University of Lubeck, Germany
    J Bacteriol 179:1344-53. 1997
    A terminal quinol oxidase has been isolated from the plasma membrane of the crenarchaeon Acidianus ambivalens (DSM 3772) (formerly Desulfurolobus ambivalens), cloned, and sequenced...
  81. ncbi Using protein synthesis inhibitors to establish the phylogenetic relationships of the Sulfolobales order
    J L Sanz
    Centro de Biologia Molecular, Universidad Autonoma de Madrid, Cantoblanco, Spain
    J Mol Evol 39:528-32. 1994
    The sensitivity of the cell-free protein synthesis systems from Acidanus brierleyi, Acidianus infernus, and Metallosphaera sedula, members of the archaeal order Sulfolobales, to 40 antibiotics with different specificities has been ..
  82. ncbi Occurrence, biochemistry and possible biotechnological application of the 3-hydroxypropionate cycle
    M Ishii
    Department of Biotechnology, Graduate School of Agricultural and Life Sciences, 1 1 1 Yayoi, Bunkyo ku, 113 8657, Tokyo, Japan
    Appl Microbiol Biotechnol 64:605-10. 2004
    ..carbon dioxide fixation, is reviewed with special emphasis on the biochemistry of CO2 fixing enzymes in Acidianus brierleyi, a thermophilic and acidophilic archeon...
  83. ncbi Acidianus tengchongensis sp. nov., a new species of acidothermophilic archaeon isolated from an acidothermal spring
    Zheng Guo He
    Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China
    Curr Microbiol 48:159-63. 2004
    A new thermoacidophilic, obligately chemolithotrophic, facultatively aerobic archaeon Acidianus S5(T), was isolated from a Tengchong acidothermal spring in southwestern China...
  84. ncbi Crystallographic analysis of the intact metal centres [3Fe-4S](1+/0) and [4Fe-4S](2+/1+) in a Zn(2+) -containing ferredoxin
    Carlos Frazao
    Instituto Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av da Republica, Oeiras, Portugal
    FEBS Lett 582:763-7. 2008
    ..The here reported, hemihedric twinned crystal structure at 2.0 A resolution from Acidianus ambivalens ferredoxin, shows an integral 103 residues, physiologically relevant native form composed by a N-..
  85. doi The dihydrolipoamide dehydrogenase from the crenarchaeon Acidianus ambivalens
    Ana P Batista
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    FEMS Microbiol Lett 281:147-54. 2008
    A dihydrolipoamide dehydrogenase (DLDH) was purified and characterized for the first time from a crenarchaeon, Acidianus ambivalens. The holoenzyme consists of two identical subunits with a molecular mass of 45.4 kDa per monomer...
  86. ncbi Studies of the molten globule state of ferredoxin: structural characterization and implications on protein folding and iron-sulfur center assembly
    Sónia S Leal
    Instituto Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal
    Proteins 68:606-16. 2007
    ..the formation of a molten globule state in the [3Fe4S][4Fe4S] ferredoxin from the thermophilic archaeon Acidianus ambivalens (AaFd), which also contains a structural zinc site...
  87. pmc Cell cycle characteristics of crenarchaeota: unity among diversity
    Magnus Lundgren
    Department of Molecular Evolution, Evolutionary Biology Center, Uppsala University, Norbyvagen 18C, SE 752 36 Uppsala, Sweden
    J Bacteriol 190:5362-7. 2008
    The hyperthermophilic archaea Acidianus hospitalis, Aeropyrum pernix, Pyrobaculum aerophilum, Pyrobaculum calidifontis, and Sulfolobus tokodaii representing three different orders in the phylum Crenarchaeota were analyzed by flow ..
  88. ncbi On the relative contribution of ionic interactions over iron-sulfur clusters to ferredoxin stability
    Sónia S Leal
    Instituto Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal
    Biochim Biophys Acta 1784:1596-600. 2008
    ..The latter however play a relevant role in determining the protein thermal stability...
  89. pmc Ferrous iron- and sulfur-induced genes in Sulfolobus metallicus
    Stephan Bathe
    Department of Biological Sciences, University of Warwick, Coventry, UK
    Appl Environ Microbiol 73:2491-7. 2007
    ..metallicus, with the predicted protein showing high identities to the previously described examples from S. tokodaii and species of Acidianus.
  90. pmc Novel thermostable Y-family polymerases: applications for the PCR amplification of damaged or ancient DNAs
    John P McDonald
    Laboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2725, USA
    Nucleic Acids Res 34:1102-11. 2006
    ..Here, we report the identification and characterization of five novel thermostable Dpo4-like enzymes from Acidianus infernus, Sulfolobus shibatae, Sulfolobus tengchongensis, Stygiolobus azoricus and Sulfurisphaera ohwakuensis, ..
  91. ncbi Viruses in acidic geothermal environments of the Kamchatka Peninsula
    Ariane Bize
    Molecular Biology of the Gene in Extremophiles Unit, Institut Pasteur, rue du Dr Roux 25, 75724 Paris Cedex 15, France
    Res Microbiol 159:358-66. 2008
    ..One of the viruses, Acidianus filamentous virus 9, AFV9, was isolated and its structure and genome were studied in detail.
  92. pmc Stygiolobus rod-shaped virus and the interplay of crenarchaeal rudiviruses with the CRISPR antiviral system
    Gisle Vestergaard
    Danish Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, DK 2200 Copenhagen N, Denmark
    J Bacteriol 190:6837-45. 2008
    ..genomic similarities to the other characterized rudiviruses Sulfolobus rod-shaped virus 1 (SIRV1), SIRV2, and Acidianus rod-shaped virus 1, isolated from hot acidic springs of Iceland and Italy...
  93. ncbi A novel rudivirus, ARV1, of the hyperthermophilic archaeal genus Acidianus
    Gisle Vestergaard
    Danish Archaea Centre, Institute of Molecular Biology, Copenhagen University, Sølvgade 83H, DK 1307 Copenhagen K, Denmark
    Virology 336:83-92. 2005
    Virus ARV1, the first member of the family Rudiviridae infecting hyperthermophilic archaea of the genus Acidianus, was isolated from a hot spring in Pozzuoli, Italy...
  94. ncbi A Rieske ferredoxin typifying a subtype within Rieske proteins: spectroscopic, biochemical and stability studies
    Arnulf Kletzin
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    FEBS Lett 579:1020-6. 2005
    ..subtype of archaeal Rieske ferredoxin (RFd) has been identified in the genome of the thermoacidophilic archaeon Acidianus ambivalens. The gene is inserted in an atypical genomic context in a gene cluster encoding a NiFe hydrogenase...
  95. ncbi Coupling of the pathway of sulphur oxidation to dioxygen reduction: characterization of a novel membrane-bound thiosulphate:quinone oxidoreductase
    Fabian H Müller
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, D 64287 Darmstadt, Germany
    Mol Microbiol 53:1147-60. 2004
    ..of the products of the initial step of the elemental sulphur oxidation pathway in the thermoacidophilic archaeon Acidianus ambivalens...
  96. ncbi Dissimilatory oxidation and reduction of elemental sulfur in thermophilic archaea
    Arnulf Kletzin
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, D 64287 Darmstadt, Germany
    J Bioenerg Biomembr 36:77-91. 2004
    ..Elemental sulfur is the electron donor in aerobic archaea like Acidianus and Sulfolobus...
  97. ncbi Acidianus, Sulfolobus and Metallosphaera surface layers: structure, composition and gene expression
    Andreas Veith
    Institute of Microbiology and Genetics, Technische Universitat Darmstadt, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Mol Microbiol 73:58-72. 2009
    ..We isolated the large S-layer protein of Acidianus ambivalens and both S-layer subunits of Sulfolobus solfataricus and Metallosphaera sedula, respectively...
  98. doi Probing the archaeal diversity of a mixed thermophilic bioleaching culture by TGGE and FISH
    Deirdre Mikkelsen
    Centre for Bacterial Diversity and Identification, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia
    Syst Appl Microbiol 32:501-13. 2009
    ..J1, Acidianus infernus and Sulfurisphaera ohwakuensis. However, the St...
  99. ncbi Active site structure of the aa3 quinol oxidase of Acidianus ambivalens
    Tapan Kanti Das
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Biochim Biophys Acta 1655:306-20. 2004
    The membrane bound aa(3)-type quinol:oxygen oxidoreductase from the hyperthermophilic archaeon, Acidianus ambivalens, which thrives at a pH of 2...
  100. doi Structural and functional insights into sulfide:quinone oxidoreductase
    José A Brito
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Av da Republica EAN, 2780 157 Oeiras, Portugal
    Biochemistry 48:5613-22. 2009
    A sulfide:quinone oxidoreductase (SQR) was isolated from the membranes of the hyperthermoacidophilic archaeon Acidianus ambivalens, and its X-ray structure, the first reported for an SQR, was determined to 2.6 A resolution...

Research Grants5

  1. X-RAY SPECTROSCOPIC STUDIES ON HEME-COPPER OXIDASES
    Ninian Blackburn; Fiscal Year: 2001
    ..First, we will investigate resting states of the CuB centers of quinol oxidases from Bacillus subtilis, Acidianus ambivalens, Thioalcalomicrobium aerophilum, and a CuA-deleted mutant of Thermus thermophilus...
  2. X-RAY SPECTROSCOPIC STUDIES ON HEME-COPPER OXIDASES
    Ninian Blackburn; Fiscal Year: 2002
    ..First, we will investigate resting states of the CuB centers of quinol oxidases from Bacillus subtilis, Acidianus ambivalens, Thioalcalomicrobium aerophilum, and a CuA-deleted mutant of Thermus thermophilus...
  3. X-RAY SPECTROSCOPIC STUDIES ON HEME-COPPER OXIDASES
    Ninian Blackburn; Fiscal Year: 2003
    ..First, we will investigate resting states of the CuB centers of quinol oxidases from Bacillus subtilis, Acidianus ambivalens, Thioalcalomicrobium aerophilum, and a CuA-deleted mutant of Thermus thermophilus...
  4. X-RAY SPECTROSCOPIC STUDIES ON HEME-COPPER OXIDASES
    Ninian Blackburn; Fiscal Year: 2004
    ..First, we will investigate resting states of the CuB centers of quinol oxidases from Bacillus subtilis, Acidianus ambivalens, Thioalcalomicrobium aerophilum, and a CuA-deleted mutant of Thermus thermophilus...
  5. X-RAY SPECTROSCOPIC STUDIES ON HEME-COPPER OXIDASES
    Ninian Blackburn; Fiscal Year: 2006
    ..First, we will investigate resting states of the CuB centers of quinol oxidases from Bacillus subtilis, Acidianus ambivalens, Thioalcalomicrobium aerophilum, and a CuA-deleted mutant of Thermus thermophilus...