dictyostelium

Summary

Summary: A genus of protozoa, formerly also considered a fungus. Its natural habitat is decaying forest leaves, where it feeds on bacteria. D. discoideum is the best-known species and is widely used in biomedical research.

Top Publications

  1. pmc Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system
    Stefan Pukatzki
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:1528-33. 2006
  2. pmc dictyBase update 2011: web 2.0 functionality and the initial steps towards a genome portal for the Amoebozoa
    Pascale Gaudet
    dictyBase, Northwestern University Biomedical Informatics Center and Center for Genetic Medicine, 420 E Superior St, Chicago, IL 60611, USA
    Nucleic Acids Res 39:D620-4. 2011
  3. pmc Phylogeny-wide analysis of social amoeba genomes highlights ancient origins for complex intercellular communication
    Andrew J Heidel
    Leibniz Institute for Age Research Fritz Lipmann Institute, D 07745 Jena, Germany
    Genome Res 21:1882-91. 2011
  4. pmc Legionella eukaryotic-like type IV substrates interfere with organelle trafficking
    Karim Suwwan de Felipe
    Integrated Program in Cellular, Molecular, and Biophysical Studies, Columbia University Medical Center, New York, New York, United States of America
    PLoS Pathog 4:e1000117. 2008
  5. ncbi Eukaryotic chemotaxis: a network of signaling pathways controls motility, directional sensing, and polarity
    Kristen F Swaney
    Department of Cell Biology, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA
    Annu Rev Biophys 39:265-89. 2010
  6. pmc The ordered extension of pseudopodia by amoeboid cells in the absence of external cues
    Leonard Bosgraaf
    Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
    PLoS ONE 4:e5253. 2009
  7. pmc The onset of collective behavior in social amoebae
    Thomas Gregor
    Graduate School of Arts and Sciences, University of Tokyo, Tokyo 153 8902, Japan
    Science 328:1021-5. 2010
  8. pmc Comparative genomics of the social amoebae Dictyostelium discoideum and Dictyostelium purpureum
    Richard Sucgang
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Genome Biol 12:R20. 2011
  9. pmc Conserved developmental transcriptomes in evolutionarily divergent species
    Anup Parikh
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Genome Biol 11:R35. 2010
  10. pmc External and internal constraints on eukaryotic chemotaxis
    Danny Fuller
    Cell and Developmental Biology, Division of Biological Sciences, Department of Physics, and Center for Theoretical Biological Physics, University of California, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 107:9656-9. 2010

Detail Information

Publications343 found, 100 shown here

  1. pmc Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system
    Stefan Pukatzki
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:1528-33. 2006
    ..Using Dictyostelium discoideum as a model host, we have identified a virulence mechanism in a non-O1/non-O139 V...
  2. pmc dictyBase update 2011: web 2.0 functionality and the initial steps towards a genome portal for the Amoebozoa
    Pascale Gaudet
    dictyBase, Northwestern University Biomedical Informatics Center and Center for Genetic Medicine, 420 E Superior St, Chicago, IL 60611, USA
    Nucleic Acids Res 39:D620-4. 2011
    dictyBase (http://www.dictybase.org), the model organism database for Dictyostelium, aims to provide the broad biomedical research community with well integrated, high quality data and tools for Dictyostelium discoideum and related ..
  3. pmc Phylogeny-wide analysis of social amoeba genomes highlights ancient origins for complex intercellular communication
    Andrew J Heidel
    Leibniz Institute for Age Research Fritz Lipmann Institute, D 07745 Jena, Germany
    Genome Res 21:1882-91. 2011
    b>Dictyostelium discoideum (DD), an extensively studied model organism for cell and developmental biology, belongs to the most derived group 4 of social amoebas, a clade of altruistic multicellular organisms...
  4. pmc Legionella eukaryotic-like type IV substrates interfere with organelle trafficking
    Karim Suwwan de Felipe
    Integrated Program in Cellular, Molecular, and Biophysical Studies, Columbia University Medical Center, New York, New York, United States of America
    PLoS Pathog 4:e1000117. 2008
    ..of many endosome-like structures containing undigested material when expressed in the protozoan host Dictyostelium discoideum...
  5. ncbi Eukaryotic chemotaxis: a network of signaling pathways controls motility, directional sensing, and polarity
    Kristen F Swaney
    Department of Cell Biology, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA
    Annu Rev Biophys 39:265-89. 2010
    ..By exploiting the genetic advantages of Dictyostelium, investigators are working out the complex network of interactions between the proteins that have been ..
  6. pmc The ordered extension of pseudopodia by amoeboid cells in the absence of external cues
    Leonard Bosgraaf
    Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
    PLoS ONE 4:e5253. 2009
    ..movement by analyzing the direction, size and timing of approximately 2000 pseudopodia that are extended by Dictyostelium cells...
  7. pmc The onset of collective behavior in social amoebae
    Thomas Gregor
    Graduate School of Arts and Sciences, University of Tokyo, Tokyo 153 8902, Japan
    Science 328:1021-5. 2010
    In the social amoebae Dictyostelium discoideum, periodic synthesis and release of extracellular cyclic adenosine 3',5'-monophosphate (cAMP) guide cell aggregation and commitment to form fruiting bodies...
  8. pmc Comparative genomics of the social amoebae Dictyostelium discoideum and Dictyostelium purpureum
    Richard Sucgang
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Genome Biol 12:R20. 2011
    ..There are four groups of dictyostelids, with the most derived being a group that contains the model species Dictyostelium discoideum.
  9. pmc Conserved developmental transcriptomes in evolutionarily divergent species
    Anup Parikh
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Genome Biol 11:R35. 2010
    ..The social amoebae Dictyostelium discoideum and Dictyostelium purpureum have similar developmental morphologies although their genomes are as ..
  10. pmc External and internal constraints on eukaryotic chemotaxis
    Danny Fuller
    Cell and Developmental Biology, Division of Biological Sciences, Department of Physics, and Center for Theoretical Biological Physics, University of California, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 107:9656-9. 2010
    ..Here we determine the chemotactic response of Dictyostelium cells to exponential gradients of varying steepness and local concentration of the chemoattractant cAMP...
  11. pmc Molecular mimicry by an F-box effector of Legionella pneumophila hijacks a conserved polyubiquitination machinery within macrophages and protozoa
    Christopher T Price
    Department of Microbiology and Immunology, College of Medicine, University of Louisville, Kentucky, USA
    PLoS Pathog 5:e1000704. 2009
    ..rapid acquisition of polyubiquitinated proteins by the Legionella-containing vacuole (LCV) within macrophages, Dictyostelium discoideum, and Acanthamoeba...
  12. pmc Transient localized patterns in noise-driven reaction-diffusion systems
    Inbal Hecht
    Center for Theoretical Biological Physics, University of California San Diego, La Jolla, California 92093 0319, USA
    Phys Rev Lett 104:158301. 2010
    ..Our work is motivated by a recent biological example showing transiently excited cell membrane regions...
  13. pmc Spatio-temporal analysis of eukaryotic cell motility by improved force cytometry
    Juan C Del Alamo
    Department of Mechanical and Aerospace Engineering, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 104:13343-8. 2007
    ..method and apply it to the analysis of the dynamics of the chemotactic migration of the amoeboid form of Dictyostelium discoideum...
  14. pmc Attenuation of phospholipid signaling provides a novel mechanism for the action of valproic acid
    Xuehua Xu
    School of Biological Sciences, Royal Holloway University of London, Egham, United Kingdom
    Eukaryot Cell 6:899-906. 2007
    ..Using the social amoeba Dictyostelium discoideum, we show that an acute effect of VPA is the inhibition of chemotactic cell movement, a process ..
  15. ncbi Chemotaxis in the absence of PIP3 gradients
    Oliver Hoeller
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Curr Biol 17:813-7. 2007
    Chemotaxing neutrophils and Dictyostelium amoebae produce in their plasma membranes the signaling lipid PI(3,4,5)P3 (PIP3) in gradients, which are orientated with the external chemotactic gradient and have been proposed to act as an ..
  16. ncbi Tumor suppressor PTEN mediates sensing of chemoattractant gradients
    Miho Iijima
    Department of Cell Biology, Johns Hopkins University, School of Medicine, 21205, Baltimore, MD, USA
    Cell 109:599-610. 2002
    ..Disruption of the PI 3-phosphatase, PTEN, in Dictyostelium discoideum dramatically prolonged and broadened the PH domain relocation and actin polymerization responses, ..
  17. pmc Persistent cell motion in the absence of external signals: a search strategy for eukaryotic cells
    Liang Li
    Department of Physics, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 3:e2093. 2008
    ..cells have a search strategy when they are beyond the range of the signal they would otherwise move toward? Here we ask if single, isolated, Dictyostelium and Polysphondylium amoebae bias their motion in the absence of external cues.
  18. pmc Cells navigate with a local-excitation, global-inhibition-biased excitable network
    Yuan Xiong
    Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 107:17079-86. 2010
    ..Our study brings together heretofore diverse observations on spontaneous cytoskeletal activity, signaling responses to temporal stimuli, and spatial gradient sensing into a unified scheme...
  19. pmc Infection by tubercular mycobacteria is spread by nonlytic ejection from their amoeba hosts
    Monica Hagedorn
    Departement de Biochimie, Faculte des Sciences, Universite de Geneve, Sciences II, 30 Quai Ernest Ansermet, CH 1211 Geneve 4, Switzerland
    Science 323:1729-33. 2009
    ..Using the social amoeba Dictyostelium as a genetically tractable host for pathogenic mycobacteria, we discovered that M. tuberculosis and M...
  20. ncbi Chemotaxis in shallow gradients is mediated independently of PtdIns 3-kinase by biased choices between random protrusions
    Natalie Andrew
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Nat Cell Biol 9:193-200. 2007
    ..multiple cell types are usually generated when existing ones bifurcate and are rarely made de novo; second, in Dictyostelium cells in shallow chemoattractant gradients, pseudopods are made at the same rate whether cells are moving up ..
  21. ncbi Single-gene greenbeard effects in the social amoeba Dictyostelium discoideum
    David C Queller
    Department of Ecology and Evolution, MS 170, Rice University, Post Office Box 1892, Houston, TX 77251 1892, USA
    Science 299:105-6. 2003
    ..The csA gene in Dictyostelium discoideum acts as a single-gene greenbeard...
  22. pmc Dictyostelium finds new roles to model
    Jeffrey G Williams
    College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Genetics 185:717-26. 2010
    ..made using the model be of wider relevance? This review addresses these issues for the social amoeba Dictyostelium and highlights some of the organisms more recent applications...
  23. pmc Genetic control of lithium sensitivity and regulation of inositol biosynthetic genes
    Jason King
    School of Biosciences, Cardiff University, Cardiff, UK
    PLoS ONE 5:e11151. 2010
    ..An alternative approach is to investigate the genetics of Li(+) sensitivity. Using the social amoeba Dictyostelium, we previously identified prolyl oligopeptidase (PO) as a modulator of Li(+) sensitivity...
  24. ncbi Dictyostelium discoideum chemotaxis: threshold for directed motion
    Loling Song
    Laboratory of Atomic and Solid State Physics, Department of Physics, Cornell University, Ithaca, NY 14853, USA
    Eur J Cell Biol 85:981-9. 2006
    The chemotactic response of Dictyostelium discoideum cells to stationary, linear gradients of cyclic adenosine 3',5'-monophosphate (cAMP) was studied using microfluidic devices...
  25. ncbi The neuroprotective agent, valproic acid, regulates the mitogen-activated protein kinase pathway through modulation of protein kinase A signalling in Dictyostelium discoideum
    Katrina Boeckeler
    Department of Biology and the Wolfson Institute for Biomedical Research, University College London, London WC1E 6BT, UK
    Eur J Cell Biol 85:1047-57. 2006
    ..To investigate the molecular basis of this effect, we have used the biomedical model system Dictyostelium discoideum to dissect this signalling pathway...
  26. ncbi Phosphoinositides differentially regulate bacterial uptake and Nramp1-induced resistance to Legionella infection in Dictyostelium
    Barbara Peracino
    Department of Clinical and Biological Sciences, University of Turin, AOU S Luigi, Reg Gonzole 10, 10043 Orbassano, Torino, Italy
    J Cell Sci 123:4039-51. 2010
    ..of PI3K, PTEN or PLC on Escherichia coli and Legionella pneumophila uptake by the professional phagocyte Dictyostelium discoideum. We show that L...
  27. pmc Autophagy genes protect against Salmonella typhimurium infection and mediate insulin signaling-regulated pathogen resistance
    Kailiang Jia
    Department of Internal Medicine, The Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 106:14564-9. 2009
    ..Serovar Typhimurium (Salmonella typhimurium) infection in 2 model organisms, Caenorhabditis elegans and Dictyostelium discoideum...
  28. ncbi Adenylyl cyclase localization regulates streaming during chemotaxis
    Paul W Kriebel
    Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Building 37 Room 1E24, Bethesda, MD 20892, USA
    Cell 112:549-60. 2003
    We studied the role of the adenylyl cyclase ACA in Dictyostelium discoideum chemotaxis and streaming. In this process, cells orient themselves in a head to tail fashion as they are migrating to form aggregates...
  29. ncbi An autoregulatory circuit for long-range self-organization in Dictyostelium cell populations
    Satoshi Sawai
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nature 433:323-6. 2005
    Nutrient-deprived Dictyostelium amoebae aggregate to form a multicellular structure by chemotaxis, moving towards propagating waves of cyclic AMP that are relayed from cell to cell...
  30. ncbi Pseudopod growth and evolution during cell movement is controlled through SCAR/WAVE dephosphorylation
    Seiji Ura
    Beatson Institute for Cancer Research, Bearsden, Glasgow, UK
    Curr Biol 22:553-61. 2012
    ..SCAR/WAVE is phosphorylated at multiple sites, but how this affects its biological activity is unclear. Here we show that dephosphorylation of Dictyostelium SCAR controls normal pseudopod dynamics.
  31. pmc Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis
    Michael Carnell
    Cancer Research UK Beatson Institute for Cancer Research, Bearsden, Glasgow G61 1BD, Scotland, UK
    J Cell Biol 193:831-9. 2011
    ..In this paper, we show that WASH coats mature Dictyostelium discoideum lysosomes and is essential for exocytosis of indigestible material...
  32. pmc The cyclase-associated protein CAP as regulator of cell polarity and cAMP signaling in Dictyostelium
    Angelika A Noegel
    Center for Biochemistry, Medical Faculty, University of Cologne, 50931 Cologne, Germany
    Mol Biol Cell 15:934-45. 2004
    ..We show that cell polarization, F-actin organization, and phototaxis are altered in a Dictyostelium CAP knockout mutant...
  33. pmc Pharmacological profiling of the Dictyostelium adenylate cyclases ACA, ACB and ACG
    Elisa Alvarez-Curto
    School of Life Sciences, University of Dundee, Dundee, Scotland, UK
    Biochem J 401:309-16. 2007
    ..crucial roles in controlling cell movement and gene regulation throughout development of the social amoeba Dictyostelium discoideum...
  34. pmc Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum
    Sarah T Miyata
    Department of Medical Microbiology and Immunology, 1 63 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Infect Immun 79:2941-9. 2011
    ..We present VasX as a novel virulence factor of the T6SS, as a V. cholerae mutant lacking vasX exhibits a phenotype of attenuated virulence toward Dictyostelium discoideum.
  35. ncbi Chemotaxis: finding the way forward with Dictyostelium
    Jason S King
    CRUK Beatson Institute, Bearsden, Glasgow, UK
    Trends Cell Biol 19:523-30. 2009
    ..This has led to the popularity of Dictyostelium discoideum, with its experimentally-friendly lifestyle and small, haploid genome, as a tool to dissect the ..
  36. ncbi Macroautophagy is dispensable for intracellular replication of Legionella pneumophila in Dictyostelium discoideum
    Grant P Otto
    Department of Anatomy and Cell Biology, Columbia University, 630 West 168th St, New York, NY 10032, USA
    Mol Microbiol 51:63-72. 2004
    ..pneumophila infection requires macroautophagy. As L. pneumophila replicates in Dictyostelium discoideum, and macroautophagy genes have been identified and mutated in D...
  37. ncbi Quantifying in vivo phosphoinositide turnover in chemotactically competent Dictyostelium cells
    Nadine Pawolleck
    Bourne Laboratory, Biomedical Sciences Division, School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, UK
    Methods Mol Biol 571:283-90. 2009
    Phosphoinositide (PI) signalling is one of multiple signalling cascades involved in chemotaxis in Dictyostelium discoideum...
  38. pmc Autophosphorylation activates Dictyostelium myosin II heavy chain kinase A by providing a ligand for an allosteric binding site in the alpha-kinase domain
    Scott W Crawley
    Department of Biochemistry, Queen s University, Kingston, Ontario K7L 3N6, Canada
    J Biol Chem 286:2607-16. 2011
    b>Dictyostelium discoideum myosin II heavy chain kinase A (MHCK A), a member of the atypical α-kinase family, phosphorylates sites in the myosin II tail that block filament assembly...
  39. ncbi Loss of Dictyostelium ATG9 results in a pleiotropic phenotype affecting growth, development, phagocytosis and clearance and replication of Legionella pneumophila
    Sze Man Tung
    Zentrum für Biochemie, Universitat zu Koln, Germany
    Cell Microbiol 12:765-80. 2010
    Infection of Dictyostelium discoideum with Legionella pneumophila resulted in a large number of differentially regulated genes among them three core autophagy genes, ATG8, ATG9 and ATG16...
  40. pmc WASP family proteins: their evolution and its physiological implications
    Douwe M Veltman
    Beatson Institute for Cancer Research, Glasgow, United Kingdom
    Mol Biol Cell 21:2880-93. 2010
    ..An unusual Dictyostelium WASP homologue that has lost the WH1 domain has retained its function in clathrin-mediated endocytosis, ..
  41. ncbi Orientation of chemotactic cells and growth cones: models and mechanisms
    H Meinhardt
    Max Planck Institut fur Entwicklungsbiologie, Spemannstr 35, D 72076 Tubingen, Germany
    J Cell Sci 112:2867-74. 1999
    ..Travelling waves or oscillations in counter phase can emerge on the cell cortex, in agreement with observations reported in the literature. In other ranges of parameters, the model accounts for the generation of a stable cell polarity...
  42. pmc Two complementary, local excitation, global inhibition mechanisms acting in parallel can explain the chemoattractant-induced regulation of PI(3,4,5)P3 response in dictyostelium cells
    Lan Ma
    Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biophys J 87:3764-74. 2004
    Chemotaxing cells, such as Dictyostelium and mammalian neutrophils, sense shallow chemoattractant gradients and respond with highly polarized changes in cell morphology and motility...
  43. pmc Exploring the control circuit of cell migration by mathematical modeling
    Javier Satulovsky
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Biophys J 94:3671-83. 2008
    ..we show that the mechanism of local stimulation global inhibition can readily account for the behavior of Dictyostelium under a large collection of conditions...
  44. ncbi Towards a molecular understanding of human diseases using Dictyostelium discoideum
    Robin S B Williams
    Department of Biology and the Wolfson Institute for Biomedical Research, University College London, London, WC1E 6BT, UK
    Trends Mol Med 12:415-24. 2006
    The social amoeba Dictyostelium discoideum is increasingly being used as a simple model for the investigation of problems that are relevant to human health...
  45. pmc Navigation of chemotactic cells by parallel signaling to pseudopod persistence and orientation
    Leonard Bosgraaf
    Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
    PLoS ONE 4:e6842. 2009
    ..Here we use a new computational algorithm to analyze the extension of pseudopods by Dictyostelium cells...
  46. pmc Self-organization of the phosphatidylinositol lipids signaling system for random cell migration
    Yoshiyuki Arai
    Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 107:12399-404. 2010
    ..waves of PtdIns 3,4,5-trisphosphate [PtdIns(3,4,5)P(3)] are generated spontaneously on the membrane of Dictyostelium cells in the absence of a chemoattractant...
  47. ncbi Collective cell migration in development
    Cornelis J Weijer
    Division of Cell and Developmental Biology, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    J Cell Sci 122:3215-23. 2009
    ....
  48. pmc The mood stabiliser lithium suppresses PIP3 signalling in Dictyostelium and human cells
    Jason S King
    Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, UK
    Dis Model Mech 2:306-12. 2009
    ..By using the genetically tractable organism Dictyostelium, we show that lithium suppresses PIP(3)-mediated signalling...
  49. ncbi The novel tyrosine kinase ZAK1 activates GSK3 to direct cell fate specification
    L Kim
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 99:399-408. 1999
    ..Inhibition of GSK3 by 7-TM Wnt/wg receptor signaling is critical for specifying embryonic cell fate patterns. In Dictyostelium, the 7-TM cAMP receptors regulate GSK3 by parallel, antagonistic pathways to establish a developmental body ..
  50. pmc Ordered patterns of cell shape and orientational correlation during spontaneous cell migration
    Yusuke T Maeda
    Department of Physics, Graduate School of Science, The University of Tokyo, Tokyo, Japan
    PLoS ONE 3:e3734. 2008
    ..Yet little is known about how cells change their own shape and how cells coordinate the deformation and movement. Here, we investigated the mechanism of spontaneous cell migration by using computational analyses...
  51. ncbi How human leukocytes track down and destroy pathogens: lessons learned from the model organism Dictyostelium discoideum
    Tian Jin
    Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, 12331 Parklawn Drive, Rockville, MD 20852, USA
    Immunol Res 43:118-27. 2009
    ..Interestingly, a soil amoeba, Dictyostelium discoideum, uses these same behaviors to pursue and injest its bacterial food source and to organize its multi-..
  52. ncbi Macroautophagy is required for multicellular development of the social amoeba Dictyostelium discoideum
    Grant P Otto
    Department of Anatomy and Cell Biology, Columbia University, New York, New York 10032, USA
    J Biol Chem 278:17636-45. 2003
    ..In Dictyostelium, development is induced by starvation, conditions under which autophagy is required for survival in yeast and ..
  53. pmc Functional analysis of spontaneous cell movement under different physiological conditions
    Hiroaki Takagi
    Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
    PLoS ONE 3:e2648. 2008
    ..For that purpose, we observed a series of spontaneous movements by Dictyostelium cells at different developmental periods by using a single cell tracking system...
  54. pmc Activated membrane patches guide chemotactic cell motility
    Inbal Hecht
    Center for Theoretical Biological Physics, University of California San Diego, La Jolla, California, United States of America
    PLoS Comput Biol 7:e1002044. 2011
    ..how these patches are related to cell motion, we examine the spatial localization of RasGTP in chemotaxing Dictyostelium discoideum cells under conditions where the vertical extent of the cell was restricted...
  55. pmc Stretching actin filaments within cells enhances their affinity for the myosin II motor domain
    Taro Q P Uyeda
    National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    PLoS ONE 6:e26200. 2011
    ..of actin filaments in vivo, we expressed GFP-fused S1 with mutations that enhanced its affinity for actin in Dictyostelium cells...
  56. pmc Involvement of the AP-1 adaptor complex in early steps of phagocytosis and macropinocytosis
    Yaya Lefkir
    Institut de Biologie et Chimie des Proteines, UMR5086 CNRS Université Lyon I, IFR 128 Biosciences Lyon Gerland, 69367 Lyon, France
    Mol Biol Cell 15:861-9. 2004
    ..Here, we show that AP-1 is also localized to phagocytic cups in murine macrophages as well as in Dictyostelium amoebae...
  57. pmc Leveraging the membrane - cytoskeleton interface with myosin-1
    Russell E McConnell
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37205, USA
    Trends Cell Biol 20:418-26. 2010
    ..These properties put myosins-1 in a unique position to regulate dynamic membrane-cytoskeleton interactions and respond to physical forces during these events...
  58. ncbi Gene discovery in the Acanthamoeba castellanii genome
    Iain J Anderson
    Department of Parasite Genomics, The Institute for Genomic Research, 9712 Medical Center Dr, Rockville, MD 20850, USA
    Protist 156:203-14. 2005
    ..The data predict that A. castellanii exhibits a greater biosynthetic capacity than the free-living Dictyostelium discoideum and the parasite Entamoeba histolytica, providing an explanation for the ability of A...
  59. pmc Overlapping functions of the two talin homologues in Dictyostelium
    Masatsune Tsujioka
    National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Eukaryot Cell 7:906-16. 2008
    ..The cellular slime mold Dictyostelium discoideum has two talin homologues, talA and talB, and earlier studies have characterized the single knockout ..
  60. pmc SCAR knockouts in Dictyostelium: WASP assumes SCAR's position and upstream regulators in pseudopods
    Douwe M Veltman
    Beatson Institute for Cancer Research, Glasgow G61 1BD, Scotland, UK
    J Cell Biol 198:501-8. 2012
    ..We show that, unexpectedly, Dictyostelium discoideum SCAR knockouts could still spread, migrate, and chemotax using pseudopods driven by the Arp2/3 ..
  61. pmc Automated characterization of cell shape changes during amoeboid motility by skeletonization
    Yuan Xiong
    Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    BMC Syst Biol 4:33. 2010
    ....
  62. ncbi A Gβγ effector, ElmoE, transduces GPCR signaling to the actin network during chemotaxis
    Jianshe Yan
    Chemotaxis Signal Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
    Dev Cell 22:92-103. 2012
    ..Here, we identified a Gβγ effector, ElmoE in Dictyostelium, and demonstrated that it is required for GPCR-mediated chemotaxis...
  63. ncbi MidA is a putative methyltransferase that is required for mitochondrial complex I function
    Sergio Carilla-Latorre
    Instituto de Investigaciones Biomedicas Alberto Sols CSIC UAM, Arturo Duperier 4, 28029 Madrid, Spain
    J Cell Sci 123:1674-83. 2010
    b>Dictyostelium and human MidA are homologous proteins that belong to a family of proteins of unknown function called DUF185...
  64. ncbi An Elmo-like protein associated with myosin II restricts spurious F-actin events to coordinate phagocytosis and chemotaxis
    Nilgun Isik
    Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, 12331 Parklawn Drive, Rockville, MD 20852, USA
    Dev Cell 15:590-602. 2008
    ..We identified an Elmo-like protein, ElmoA, in Dictyostelium discoideum that unexpectedly functions as a negative regulator of actin polymerization...
  65. ncbi Propagating chemoattractant waves coordinate periodic cell movement in Dictyostelium slugs
    D Dormann
    School of Life Sciences, Division of Cell and Developmental Biology, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK
    Development 128:4535-43. 2001
    Migration and behaviour of Dictyostelium slugs results from coordinated movement of its constituent cells. It has been proposed that cell movement is controlled by propagating waves of cAMP as during aggregation and in the mound...
  66. ncbi Primitive agriculture in a social amoeba
    Debra A Brock
    Department of Ecology and Evolutionary Biology, Rice University, 6100 Main Street, Houston, Texas 77005, USA
    Nature 469:393-6. 2011
    ..Here we show that the social amoeba Dictyostelium discoideum has a primitive farming symbiosis that includes dispersal and prudent harvesting of the crop...
  67. pmc Dictyostelium chemotaxis: essential Ras activation and accessory signalling pathways for amplification
    Arjan Kortholt
    Department of Cell Biochemistry, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands
    EMBO Rep 12:1273-9. 2011
    ..We used Dictyostelium mutants to investigate the minimal requirements for chemotaxis, and identified a basal signalling module ..
  68. ncbi A common mechanism of action for three mood-stabilizing drugs
    Robin S B Williams
    Intracellular Signalling Group, MRC Laboratory for Molecular Cell Biology, University College London, Gower St, London WC1E 6BT, UK
    Nature 417:292-5. 2002
    ..Moreover, the development of Dictyostelium is sensitive to lithium and to valproic acid, but resistance to both is conferred by deletion of the gene that ..
  69. pmc The antiepileptic drug valproic acid and other medium-chain fatty acids acutely reduce phosphoinositide levels independently of inositol in Dictyostelium
    Pishan Chang
    Centre for Biomedical Sciences, School of Biological Sciences, Royal Holloway University of London, Egham, TW20 0EX, UK
    Dis Model Mech 5:115-24. 2012
    ..work identified a previously unknown effect of VPA in reducing phosphoinositide production in the simple model Dictyostelium followed by the transfer of data to a mammalian synaptic release model...
  70. ncbi The in vivo role of annexin VII (synexin): characterization of an annexin VII-deficient Dictyostelium mutant indicates an involvement in Ca(2+)-regulated processes
    V Doring
    Max Planck Institut fur Biochemie, Martinsried, FRG
    J Cell Sci 108:2065-76. 1995
    b>Dictyostelium discoideum cells harbor two annexin VII isoforms of 47 and 51 kDa which are present throughout development...
  71. ncbi Cloning and functional expression of UGT genes encoding sterol glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideum
    D Warnecke
    Universitat Hamburg, Institut fur Allgemeine Botanik, 22609 Hamburg, Germany
    J Biol Chem 274:13048-59. 1999
    ..4.1.173). We cloned genes from three different yeasts and from Dictyostelium discoideum, the deduced amino acid sequences of which all showed similarities with plant sterol ..
  72. ncbi Coupling mechanism of a GPCR and a heterotrimeric G protein during chemoattractant gradient sensing in Dictyostelium
    Xuehua Xu
    Chemotaxis Signal Section, National Institutes of Health, Rockville, MD 20852, USA
    Sci Signal 3:ra71. 2010
    ..We suggest that chemoattractant receptors use a ligand-induced coupling rather than a precoupled mechanism to control the activation of G proteins during chemotaxis...
  73. pmc Use of a Dictyostelium model for isolation of genetic loci associated with phagocytosis and virulence in Klebsiella pneumoniae
    Yi Jiun Pan
    Department of Microbiology, National Taiwan University College of Medicine, 1, Sec 1, Jen Ai Rd, Taipei 10051, Taiwan
    Infect Immun 79:997-1006. 2011
    Phagocytosis resistance is an important virulence factor in Klebsiella pneumoniae. Dictyostelium has been used to study the interaction between phagocytes and bacteria because of its similarity to mammalian macrophages...
  74. ncbi Regulation of PTEN by Rho small GTPases
    Zhong Li
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Nat Cell Biol 7:399-404. 2005
    ..PTEN's intracellular localization and presumably activity are regulated by chemoattractants in Dictyostelium and mouse neutrophils. However, the mechanisms for its regulation remain elusive...
  75. pmc A role for tRNA(His) guanylyltransferase (Thg1)-like proteins from Dictyostelium discoideum in mitochondrial 5'-tRNA editing
    Maria G Abad
    Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    RNA 17:613-23. 2011
    ..Here we investigate the activities of four Thg1-like proteins (TLPs) encoded by the genome of the slime mold, Dictyostelium discoideum (a member of the eukaryotic supergroup Amoebozoa)...
  76. pmc A Rap/phosphatidylinositol 3-kinase pathway controls pseudopod formation [corrected]
    Arjan Kortholt
    Department of Molecular Cell Biology, University of Groningen, 9751 NN Haren, The Netherlands
    Mol Biol Cell 21:936-45. 2010
    GbpD, a Dictyostelium discoideum guanine exchange factor specific for Rap1, has been implicated in adhesion, cell polarity, and chemotaxis...
  77. ncbi C-sequence of the Dictyostelium cytoplasmic dynein participates in processivity modulation
    Naoki Numata
    Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3 8 1 Komaba, Meguro ku, Tokyo, Japan
    FEBS Lett 585:1185-90. 2011
    We examined the functional roles of C-sequence, a 47-kDa non-AAA+ module at the C-terminal end of the 380-kDa Dictyostelium dynein motor domain...
  78. pmc Dictyostelium Aurora kinase has properties of both Aurora A and Aurora B kinases
    Hui Li
    Section of Molecular Cell and Developmental Biology, University of Texas at Austin, Austin, TX 78712, USA
    Eukaryot Cell 7:894-905. 2008
    ..We show that Dictyostelium discoideum contains a single Aurora kinase, DdAurora, that displays characteristics of both Aurora A and B...
  79. pmc Differentiation-inducing factor-1 and -2 function also as modulators for Dictyostelium chemotaxis
    Hidekazu Kuwayama
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
    PLoS ONE 4:e6658. 2009
    In the early stages of development of the cellular slime mold Dictyostelium discoideum, chemotaxis toward cAMP plays a pivotal role in organizing discrete cells into a multicellular structure...
  80. ncbi Control of cell polarity and chemotaxis by Akt/PKB and PI3 kinase through the regulation of PAKa
    C Y Chung
    Section of Cell and Developmental Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0634, USA
    Mol Cell 7:937-47. 2001
    ..Addition of the PI3K inhibitor LY294002 results in a rapid loss of cell polarity and the axial distribution of actin, myosin, and PAKa. These results provide a mechanism by which PI3K regulates chemotaxis...
  81. ncbi Proteasomes from Dictyostelium discoideum: characterization of structure and function
    T M Schauer
    Max Planck Institute for Biochemistry, Martinsried, Germany
    J Struct Biol 111:135-47. 1993
    We have isolated and purified 20 S proteasomes from Dictyostelium discoideum and characterized their proteolytic activities. Two-dimensional electrophoresis revealed 13 distinct spots...
  82. pmc The group migration of Dictyostelium cells is regulated by extracellular chemoattractant degradation
    Gene L Garcia
    Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 20:3295-304. 2009
    Starvation of Dictyostelium induces a developmental program in which cells form an aggregate that eventually differentiates into a multicellular structure...
  83. ncbi Glycogen synthase kinase 3 regulates cell fate in Dictyostelium
    A J Harwood
    Medical Research Council, Laboratory of Molecular Biology, Cambridge, England
    Cell 80:139-48. 1995
    Extracellular cyclic AMP (cAMP) induces the formation of prespore cells in Dictyostelium but inhibits stalk cell formation...
  84. ncbi Functional plasticity of CH domains
    Mario Gimona
    Department of Cell Biology, Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg, Austria
    FEBS Lett 513:98-106. 2002
    ....
  85. ncbi Molecular dynamics and forces of a motile cell simultaneously visualized by TIRF and force microscopies
    Yoshiaki Iwadate
    Department of Functional Molecular Biology, Graduate School of Medicine, Yamaguchi University, Yamaguchi, Japan
    Biotechniques 44:739-50. 2008
    ..detection of the spatiotemporal distribution of traction forces produced by individual filopodia in migrating Dictyostelium cells, as well as simultaneous visualization of these traction forces and the dynamics of filamentous myosin II...
  86. ncbi Changing directions in the study of chemotaxis
    Robert R Kay
    MRC Laboratory of Molecular Biology, Hill Road, Cambridge CB2 0QH, UK
    Nat Rev Mol Cell Biol 9:455-63. 2008
    ....
  87. pmc Xpf and not the Fanconi anaemia proteins or Rev3 accounts for the extreme resistance to cisplatin in Dictyostelium discoideum
    Xiao Yin Zhang
    Medical Research Council, Laboratory for Molecular Biology, Cambridge, United Kingdom
    PLoS Genet 5:e1000645. 2009
    Organisms like Dictyostelium discoideum, often referred to as DNA damage "extremophiles", can survive exposure to extremely high doses of radiation and DNA crosslinking agents...
  88. pmc Diverse cytopathologies in mitochondrial disease are caused by AMP-activated protein kinase signaling
    Paul B Bokko
    Department of Microbiology, La Trobe University, Melbourne, Victoria 3086, Australia
    Mol Biol Cell 18:1874-86. 2007
    ..a pharmacological AMPK activator in mammalian cells, mimics mitochondrial disease in impairing Dictyostelium phototaxis and that AMPKalpha antisense-inhibited cells were resistant to this effect...
  89. ncbi Formation of Hirano bodies in Dictyostelium and mammalian cells induced by expression of a modified form of an actin-crosslinking protein
    Andrew G Maselli
    Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    J Cell Sci 115:1939-49. 2002
    ..activated actin binding and calcium-insensitive actin filament crosslinking activity were expressed in Dictyostelium and mammalian cells to assess the behavior of these modified forms in vivo...
  90. pmc The role of cell contraction and adhesion in dictyostelium motility
    Mathias Buenemann
    Center for Theoretical Biological Physics, University of California, San Diego, La Jolla, California, USA
    Biophys J 99:50-8. 2010
    The crawling motion of Dictyostelium discoideum on substrata involves a number of coordinated events including cell contractions and cell protrusions...
  91. pmc Identifying an uptake mechanism for the antiepileptic and bipolar disorder treatment valproic acid using the simple biomedical model Dictyostelium
    Nicole Terbach
    Centre for Biomedical Sciences, School of Biological Sciences, Royal Holloway University of London, Egham TW200EX, UK
    J Cell Sci 124:2267-76. 2011
    ..of cellular, molecular and genetic approaches to characterize VPA uptake using a simple biomedical model, Dictyostelium discoideum. We show that VPA is taken up against an electrochemical gradient in a dose-dependent manner...
  92. ncbi Myosin II-independent cytokinesis in Dictyostelium: its mechanism and implications
    T Q Uyeda
    Biomolecular Research Group, National Institute for Advanced Interdisciplinary Research, Tsukuba, Ibaraki, Japan
    Cell Struct Funct 25:1-10. 2000
    Similar to higher animal cells, ameba cells of the cellular slime mold Dictyostelium discoideum form contractile rings containing filaments of myosin II during mitosis, and it is generally believed that contraction of these rings bisects ..
  93. pmc Adhesion-dependent and contractile ring-independent equatorial furrowing during cytokinesis in mammalian cells
    Masamitsu Kanada
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
    Mol Biol Cell 16:3865-72. 2005
    ..Nonetheless, myosin II-null cells of the cellular slime mold Dictyostelium divide efficiently when adhering to substrates by making use of polar traction forces...
  94. ncbi Receptor-dependent and tyrosine phosphatase-mediated inhibition of GSK3 regulates cell fate choice
    Leung Kim
    Laboratory of Cellular and Developmental Biology, Building 50 3351, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 3:523-32. 2002
    Asymmetric body axis formation is central to metazoan development. Dictyostelium establishes an anterior/posterior axis utilizing seven-transmembrane cAMP morphogen receptors (CARs) and GSK3-mediated signal transductions that has a ..
  95. ncbi Dictyostelium myosin-IE is a fast molecular motor involved in phagocytosis
    Ulrike Dürrwang
    Abteilung Biophysik, Max Planck Institut fur medizinische Forschung, Jahnstr 29, 69120 Heidelberg, Germany
    J Cell Sci 119:550-8. 2006
    ..A complete analysis of the kinetic and motor properties of Dictyostelium discoideum myosin-IE was achieved by the use of motor domain constructs with artificial lever arms...
  96. ncbi The Parkinson disease gene LRRK2: evolutionary and structural insights
    Ignacio Marín
    Departamento de Genetica, Universidad de Valencia, Burjassot, Spain
    Mol Biol Evol 23:2423-33. 2006
    ..Genes in the slime mold Dictyostelium discoideum with structures very similar to those found in animal LRRK genes, including the protein kinase ..
  97. pmc Dictyostelium discoideum as a model system for identification of Burkholderia pseudomallei virulence factors
    Benjamin M Hasselbring
    Department of Microbiology, 210 Biological Sciences Building, University of Georgia, Athens, GA 30602, USA
    Infect Immun 79:2079-88. 2011
    ..the closely related nearly avirulent species Burkholderia thailandensis to predation by the phagocytic amoeba Dictyostelium discoideum...
  98. ncbi Sensitization of Dictyostelium chemotaxis by phosphoinositide-3-kinase-mediated self-organizing signalling patches
    Marten Postma
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Cell Sci 117:2925-35. 2004
    The leading edge of Dictyostelium cells in chemoattractant gradients can be visualized using green fluorescent protein (GFP) tagged to the pleckstrin-homology (PH) domain of cytosolic regulator of adenylyl cyclase (CRAC), which ..
  99. ncbi Dictyostelium discoideum--a model for many reasons
    Sarah J Annesley
    Department of Microbiology, La Trobe University, Bundoora, VIC 3086, Australia
    Mol Cell Biochem 329:73-91. 2009
    The social amoeba or cellular slime mould Dictyostelium discoideum is a "professional" phagocyte that has long been recognized for its value as a biomedical model organism, particularly in studying the actomyosin cytoskeleton and ..
  100. pmc Myosin II is essential for the spatiotemporal organization of traction forces during cell motility
    Ruedi Meili
    Section of Cell and Developmental Biology, Division of Biological Sciences, Department of Mechanical and Aerospace Engineering, and Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 21:405-17. 2010
    ..However, it does not reduce significantly the magnitude of the traction forces, uncovering a non-MyoII-mediated mechanism for the contractility of the cell...
  101. pmc Chemotaxis, chemokine receptors and human disease
    Tian Jin
    Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, 12441 Parklawn Drive, Rockville, MD 20852, USA
    Cytokine 44:1-8. 2008
    ..In this review, we wish to mention landmark discoveries in the chemotaxis research field that shaped our current understanding of this fundamental cell behavior and lay out key questions that remain to be addressed in the future...

Research Grants66

  1. The role of polyphosphate and acidocalcisomes in Trypanosoma brucei
    Roberto Docampo; Fiscal Year: 2013
    ..several pathogenic microorganisms as well as in the green alga Chlamydomonas reinhardtii, and the slime mold Dictyostelium discoideum...
  2. The Molecular Basis of Allorecognition in Social Amoeba
    Gad Shaulsky; Fiscal Year: 2013
    ..In the previous grant period we found an allorecognition system in the social soil amoeba Dictyostelium discoideum...
  3. Role of mucin-type O-glycosylation in Trypanosoma cruzi biology
    Christopher M West; Fiscal Year: 2012
    ..cruzi will be analyzed, by testing its predicted 13GalT activity after heterologous expression in Dictyostelium, and investigating its biochemical and cellular function by targeted gene disruption and over-expression in T...
  4. Molecular Mechanisms of Host Function Exploitation by Type IV effectors of Legion
    Zhao Qing Luo; Fiscal Year: 2013
    ..was defective in intracellular growth in both mouse bone marrow-derived macrophages and the amoebae host Dictyostelium discoideum...
  5. Cellular and Biochemical Studies of Myosin Assembly in Dictyostelium
    Thomas Egelhoff; Fiscal Year: 2009
    ..We are using the simple amoeba Dictyostelium discoideum as a model system for identifying signaling pathways that regulate myosin assembly...
  6. Quantitative Analysis of Chemotactic Motility Cycle of Ameboid Cells
    JUAN CARLOS LASHERAS; Fiscal Year: 2013
    ..b>Dictyostelium discoideum has proven to be an excellent model for the chemotactic migration of amoeboid cells such as ..
  7. Negative regulation of virulence in Pseudomonas aeruginosa
    Albert Siryaporn; Fiscal Year: 2013
    ..in cells that are attached to abiotic surfaces Surface-attached cells are also more virulent towards the host Dictyostelium discoideum. I will test the hypothesis that virulence is regulated by surface attachment...
  8. CpnA's role in cAMP signaling and the actin cytoskeleton in Dictyostelium
    Cynthia K Damer; Fiscal Year: 2013
    ..The model organism Dictyostelium discoideum has six copine genes (cpnA-cpnF) and provides an ideal system for studying copine function...
  9. Interaction between Vibrio cholerae and Dictylostelium discoideum
    SHELLEY M contact PAYNE; Fiscal Year: 2010
    ..Our studies will focus on the relationship between V. cholerae and the model protozoan amoeba Dictyostelium discoideum, and our laboratories have combined expertise in these two organisms. Our hypothesis is that V...
  10. Lipid Signaling in Chemotaxis
    Miho Iijima; Fiscal Year: 2013
    ..Using Dictyostelium discoideum as our experimental model system, we have demonstrated that phosphatidylinositol 3,4,5 triphosphate ..
  11. Temporal and Spatial Signaling in Chemotaxis
    Peter N Devreotes; Fiscal Year: 2013
    ..How do G-proteins link to the network and what is the inhibitory mechanism that causes adaptation? Studies of Dictyostelium amoebae are designed to address these central questions, with the results assessed for generality in human ..
  12. Structural and functional effects of oxidative modification of myosin
    REBECCA J MOEN; Fiscal Year: 2013
    ..I will test this hypothesis by performing site-directed Met mutagenesis on myosin in Dictyostelium cell culture, then performing functional and structural tests to determine the correlation between structural ..
  13. Glycoregulation of Skp1 in the cytoplasm and nucleus
    Christopher M West; Fiscal Year: 2012
    ..The initial organism of analysis is the social amoeba Dictyostelium, and the target of the pathway studied here is Skp1, an adaptor of the SCF class of E3 ubiquitin ligases whose ..
  14. Actions of the Sodium-Hydrogen Exchanger Subtype, NHE1
    Diane L Barber; Fiscal Year: 2012
    ..We showed a leading-edge H+ efflux by NHE1 in mammalian fibroblasts and in Dictyostelium cells is necessary for three stages in cell migration: polarity, actin filament assembly driving membrane ..
  15. Cytosolic Proline Hydroxylation and Glycosylation
    Christopher M West; Fiscal Year: 2013
    ..Considerable evidence indicates that CRL Ub-ligases are also regulated. Our findings in the model organism Dictyostelium reveal new and novel mechanisms for regulating the SCF class itself...
  16. PI(3,4,5)P3 Regulates Cell Polarity During Chemotaxis and Cytokinesis
    Christopher Janetopoulos; Fiscal Year: 2012
    ..We are using the amoeba Dictyostelium as a model system to understand the basic mechanisms that control these changes in cell shape...
  17. CATENINS, CADHERINS AND THEIR INTERACTIONS
    William I Weis; Fiscal Year: 2012
    ..of cell adhesion assemblies will be studied by examining homologs of 1- and 2- catenin in the slime mold Dictyostelium discoideum. 3...
  18. Determinants for the assembly and maturation of clathrin coated vesicles.
    THERESA JOYCE O'HALLORAN; Fiscal Year: 2013
    ..Using Dictyostelium cells as a model system, three specific aims are proposed: (1) To define how Hip1r regulates coupling of actin ..
  19. Spatial regulation of cytoskeleton during chemotaxis
    Chang Y Chung; Fiscal Year: 2012
    ..is necessary for many biological functions, including wound healing, axonal guidance, and the aggregation of Dictyostelium cells. Chemotaxis also plays a role in disease states such as arthritis, cancer, and multiple sclerosis...
  20. Cellular Regulation of Dictyostelium Myosin Heavy Chain Kinases
    Paul A Steimle; Fiscal Year: 2012
    ..cellular migration) can be regulated to newly indentified candidate myosin II heavy chain kinases (MHCKs) in Dictyostelium, and by extension, how these processes can go awry in cancer cells exhibiting uncontrolled cell division and ..
  21. Signaling Networks in Chemotaxis and Cytokinesis
    Peter N Devreotes; Fiscal Year: 2013
    ..Studies oi Dictyostelium discoideum, with its accessible biochemistry, cell biology, and genetics, have contributed enormously to the ..
  22. The Dictyostelium Stock Center
    Rex L Chisholm; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The simple eukaryote Dictyostelium discoideum is a model organism widely used for the study of cellular processes such as cell motility, chemotaxis, cellular differentiation, signal transduction, the ..
  23. FUNCTIONAL GENOMICS OF DICTYOSTELIUM
    Blaz Zupan; Fiscal Year: 2013
    ..Program Project is aimed at understanding the mechanisms that control growth and multicellular development in Dictyostelium. In the previous project period we took a functional genomics approach to high-throughput mutant phenotyping, ..
  24. Study of Cell Adhesion Through an Analysis of Myosin 7
    Margaret A Titus; Fiscal Year: 2010
    ..conserved unconventional myosin, myosin VII (M7), in amoeboid adhesion has been discovered in the amoeba Dictyostelium. The goal of this project is to define the role of M7 amoeboid adhesion...
  25. Development of DictyBase, an online informatics resource
    Rex L Chisholm; Fiscal Year: 2013
    ..dictyBase is the model organism database that uses the genome sequence of Dictyostelium discoiedum to organize biological knowledge resulting from studies using Dictyostelium and related species...
  26. Mining a collection of soil amoebae for antibacteria agents
    Marcin S Filutowicz; Fiscal Year: 2012
    ..b>Dictyostelium discoideum exhibits the largest repository of polyketide synthase (PKS) enzymes of all known genomes...
  27. Mechanisms of cancer cell chemotaxis in perineural invasion
    Richard J Wong; Fiscal Year: 2013
    ..Mechanisms of chemotaxis have been previously defined primarily in the non-malignant systems of dictyostelium discoideum (slime mold) and leukocytes...
  28. SIGNALLING PROCESSES IN DICTYOSTELIUM
    Richard A Firtel; Fiscal Year: 2013
    ..Naive, randomly moving cells, such as leukocytes and Dictyostelium cells must be able to directionally sense extremely shallow extracellular chemoattractant gradients and ..
  29. An open microfluidic device to study chemotaxis of eukaryotic cells
    Dawit Jowhar; Fiscal Year: 2012
    ..These studies will use the social amoeba Dictyostelium discoideum and will be conducted using a newly developed open microfluidic device made out of ..
  30. Chemical biology of bacterial symbionts
    Jon Clardy; Fiscal Year: 2013
    ..several genera - Pseudomonas, Burkholderia, and Flavobacteria among others - are used by some social amoebas (Dictyostelium discoideum) to defend territory, deter predators, and provide a selective advantage over close relatives ..
  31. Intracellular signaling during phagocytosis
    Kevin Swier; Fiscal Year: 2012
    ..The PI was recently introduced to the model organism Dictyostelium discoideum and has been using this organism to introduce students in classrooms and summer workshops to basic ..
  32. Textpresso information retrieval and extraction system for biological literature
    Hans Michael Muller; Fiscal Year: 2012
    ..with the respective model organism databases, we will set up literature search engine for zebrafish, rat and Dictyostelium and consider systems for important diseases such as cancer, Alzheimer's and AIDS...
  33. An autocrine repressor of cell proliferation
    Richard H Gomer; Fiscal Year: 2009
    ..The PI has found that growing Dictyostelium cells secrete a protein called AprA for autocrine proliferation repressor...
  34. Elucidation of a Dictyostelium chalone
    Richard H Gomer; Fiscal Year: 2013
    ..In the simple eukaryotic model system Dictyostelium discoideum, cells stop proliferating when they reach a high cell density even if adequate nutrients are presen+..
  35. The Role of COTL1 in T cell development and activation
    Virginia Smith Shapiro; Fiscal Year: 2013
    ..Coactosin was initially identified in Dictyostelium, as an inhibitor of actin capping, but its function in T cells was unknown...
  36. The role of kin-recognition in social evolution
    Gad Shaulsky; Fiscal Year: 2013
    ..mechanism, which is mediated by a pair of polymorphic cell-cell adhesion proteins in the social amoeba Dictyostelium discoideum. We have also discovered dozens of mutants that cheat through various molecular mechanisms...
  37. Using Controlled 2D and 3D Nanotopography to Unravel Tactile Senses of Motile Cel
    Wolfgang Losert; Fiscal Year: 2013
    ..We propose to use the model organism Dictyostelium discoideum to understand, how nanotopographic cues affect cell migration at many levels, from the molecular ..
  38. MYOSIN ISOZYMES
    HUGH LEE SWEENEY; Fiscal Year: 2010
    ..We will make use of the fact that myosin V, non-muscle myosin MB, smooth muscle myosin II and Dictyostelium myosin II have kinetics that are fundamentally different from each other...
  39. A Toolkit for Light-Control of Molecular Processes in Living Cells
    ORION DAVID WEINER; Fiscal Year: 2013
    ..We have demonstrated that this system works in yeast, Dictyostelium, and mammalian cells...
  40. Excitability in Dictyostelium Development
    DAVID JASON SCHWAB; Fiscal Year: 2013
    ..development and how can we manipulate, disrupt, or correct them? I propose to study the collective behavior of Dictyostelium discoideum - a classic model organism for cell-cell signaling - focusing on how single-cell dynamics influence ..
  41. PP2A regulates development and stress responses
    Leung Kim; Fiscal Year: 2012
    ..In contrast, Dictyostelium discoideum, a facultative multicellular organism, has a single B56 homologue and cells lacking B56 were ..
  42. Controlling collective behavior in eukaryotic cell populations
    Thomas Gregor; Fiscal Year: 2013
    ..dynamics of single cells give rise to coherent behaviors at the multi-cell stage in the social amoeba Dictyostelium discoideum...
  43. Pathogenesis to cooperation in the microbiome of a soil amoeba
    SUSANNE DISALVO; Fiscal Year: 2013
    ..Interestingly, carrier clones of the social amoeba Dictyostelium discoideum have been shown to harbor a variety of bacteria, in contrast to their non-carrier counterparts...
  44. INTRACELLULAR ROLE OF CLATHRIN AND ITS REGULATION
    THERESA O HALLORAN; Fiscal Year: 2007
    ..Our experimental strategy is to use Dictyostelium as a model system...
  45. Chemotaxis in Dictyostelium discoideum: an integrative approach
    Wouter Jan Rappel; Fiscal Year: 2013
    ..main aim of this Program Project is to extend our quantitative studies of chemotaxis using the model system Dictyostelium discoideum...
  46. METABOLIC DETERMINANTS OF HEMATOPOIETIC CELL DEVELOPMENT
    Daniel Wright; Fiscal Year: 1999
    ..In lower eukaryotes, e.g. S. cerevisiae and Dictyostelium, developmental switches are triggered by metabolic changes caused by nutrient deprivation, and studies of ..
  47. BIOCHEMICAL BASIS OF DEVELOPMENT IN DICTYOSTELIUM
    William Loomis; Fiscal Year: 1992
    Techniques for the reproducible transformation of Dictyostelium cells with cloned sequences have allowed us to determine essential cis-acting sequences in the 5' flanking region of a developmentally controlled gene (actin 15) that are ..
  48. SEQUENCING THE DICTYOSTELIUM GENOME
    Adam Kuspa; Fiscal Year: 2004
    ..b>Dictyostelium is a social amoeba whose study has contributed to our understanding of diverse biological processes common to ..
  49. Molecular Basis for Protein-Phospholipid Interaction
    Jon Lomasney; Fiscal Year: 2005
    ..C (PLC), and the small G protein ARF; as a regulator of the actin cytoskeleton directing cytokinesis in Dictyostelium, and acting as a regulator of endo and exocytosis...
  50. Characterization of single-cell dynamics that lead to collective behaviors
    Allyson E Sgro; Fiscal Year: 2013
    ..contribute to the coordination of collective behaviors in multicellular systems such as the social amoeba Dictyostelium discoideum...
  51. Microbial Pattern Recognition by the Social Amoeba Dictyostelium discoideum - Mod
    MICHELLE LYNN DYKSTRA SNYDER; Fiscal Year: 2010
    ..Our proposal here to use the social amoeba Dictyostelium discoideum as a model system to study the molecular mechanisms underlying microbial pattern recognition in ..
  52. MOLECULAR ANALYSIS OF CYTOKINESIS IN DICTYOSTELIUM
    Arturo De Lozanne; Fiscal Year: 2006
    ..Toward that goal, three proteins required for cytokinesis in Dictyostelium have been identified...
  53. CYTOKINESIS IN DICTYOSTELIUM--A MOLECULAR ANALYSIS
    James Spudich; Fiscal Year: 2001
    ..Key to a long term undertaking to solve this problem is the judicious choice of a model system. Dictyostelium discoideum is a model of choice because it's behavioral characteristics during mitosis and cytokinesis are ..
  54. DICTYOSTELIUM GA2:CELLULAR LOCALIZATION & PALMITOYLATION
    ROBERT GUNDERSEN; Fiscal Year: 2000
    DESCRIPTION (from applicant's abstract): The protozoan, Dictyostelium discoideum, provides a powerful system in which to examine fundamental signal transduction processes of the eukaryotic cell...
  55. CHEMISTRY AND FUNCTION OF DICTYOSTELIUM CALMODULIN
    Margaret Clarke; Fiscal Year: 1992
    ..This project would characterize calmodulin from the eukaryotic microorganism Dictyostelium discoideum and explore its roles, particularly in the regulation of motility...
  56. COMPUTER ANALYSIS OF AGING IN DICTYOSTELIUM
    Barbara Wright; Fiscal Year: 1992
    ..events during the metabolic transitions essential to differentiation, aging and death in the model system, Dictyostelium discoideum...
  57. Role of cGMP signaling in Dictyostelium chemotaxis
    Janet Smith; Fiscal Year: 2007
    ..such as wound healing, embryogenesis, axonal guidance, metastasis of cancer cells, and aggregation of Dictyostelium cells to form a multicellular organism...
  58. REGULATION OF SIGNAL TRANSDUCTION IN DICTYOSTELIUM
    CHARLES SAXE; Fiscal Year: 2000
    ..A large number of G protein-linked receptors have been characterized, from organisms as diverse as yeast, Dictyostelium, Drosophila and humans, and include multi gene families for opsins, and the adrenergic, muscarinic ..
  59. RACE PATHWAY IN THE REGULATION OF CYTOKINESIS
    Denis Larochelle; Fiscal Year: 1999
    ..Recently, the eukaryotic organism Dictyostelium discoideum has emerged as one of the premier model systems for studying cytokinesis...
  60. An optogenetic method to rapidly and reversibly titrate protein levels in cells
    ORION DAVID WEINER; Fiscal Year: 2013
    ..We propose to establish and optimize this approach in 3 model systems - Dictyostelium, budding yeast, and mammalian cells to test the modularity of this system, demonstrate its versatility, and ..
  61. DEVELOPMENTAL GENE PROGRAM
    William Loomis; Fiscal Year: 1999
    ..Recent advances in molecular genetic techniques in Dictyostelium make this a favored organism for detailed studies on genes involved in cellular differentiation and ..
  62. Mechanisms of Self/Nonself Recognition in Dictyostelium discoideum
    ROCIO BENABENTOS; Fiscal Year: 2009
    ..The social amoeba Dictyostelium possess a self/nonself recognition mechanism based on cell adhesion and signaling that promotes the ..
  63. MECHANISMS OF ETHANOL TOXICITY IN DICTYOSTELIUM
    Gad Shaulsky; Fiscal Year: 2001
    ..is to identify molecular targets for ethanol toxicity by means of genetic screens in the model organism Dictyostelium discoideum...
  64. EUKARYOTIC TISSUE DENSITY SENSOR
    Richard Gomer; Fiscal Year: 1992
    ..As a model system, we will use the conditioned medium factor (CMF) secreted by developing Dictyostelium discoideum cells...
  65. Analysis of signaling during chemotaxis in Dictyostelium
    STACEY WILLARD; Fiscal Year: 2007
    ..Studies in Dictyostelium have improved our understanding of the signaling network that governs chemotaxis...
  66. REGULATION OF DICTYOSTELIUM MYOSIN BY PHOSPHORYLATION
    James Spudich; Fiscal Year: 2000
    ..The specific aim of this proposal is to study the roles of myosin phosphorylation in Dictyostelium, an organism that allows the convergence of biochemical, molecular genetic, and physiological approaches...