dictyostelium

Summary

Summary: A genus of protozoa, formerly also considered a fungus. Its natural habitat is decaying forest leaves, where it feeds on bacteria. D. discoideum is the best-known species and is widely used in biomedical research.

Top Publications

  1. ncbi Cells navigate with a local-excitation, global-inhibition-biased excitable network
    Yuan Xiong
    Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 107:17079-86. 2010
  2. ncbi Legionella eukaryotic-like type IV substrates interfere with organelle trafficking
    Karim Suwwan de Felipe
    Integrated Program in Cellular, Molecular, and Biophysical Studies, Columbia University Medical Center, New York, New York, United States of America
    PLoS Pathog 4:e1000117. 2008
  3. ncbi The ordered extension of pseudopodia by amoeboid cells in the absence of external cues
    Leonard Bosgraaf
    Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
    PLoS ONE 4:e5253. 2009
  4. ncbi Chemotaxis in the absence of PIP3 gradients
    Oliver Hoeller
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Curr Biol 17:813-7. 2007
  5. ncbi Spatio-temporal analysis of eukaryotic cell motility by improved force cytometry
    Juan C Del Alamo
    Department of Mechanical and Aerospace Engineering, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 104:13343-8. 2007
  6. ncbi Molecular mimicry by an F-box effector of Legionella pneumophila hijacks a conserved polyubiquitination machinery within macrophages and protozoa
    Christopher T Price
    Department of Microbiology and Immunology, College of Medicine, University of Louisville, Kentucky, USA
    PLoS Pathog 5:e1000704. 2009
  7. ncbi Attenuation of phospholipid signaling provides a novel mechanism for the action of valproic acid
    Xuehua Xu
    School of Biological Sciences, Royal Holloway University of London, Egham, United Kingdom
    Eukaryot Cell 6:899-906. 2007
  8. ncbi Transient localized patterns in noise-driven reaction-diffusion systems
    Inbal Hecht
    Center for Theoretical Biological Physics, University of California San Diego, La Jolla, California 92093 0319, USA
    Phys Rev Lett 104:158301. 2010
  9. ncbi External and internal constraints on eukaryotic chemotaxis
    Danny Fuller
    Cell and Developmental Biology, Division of Biological Sciences, Department of Physics, and Center for Theoretical Biological Physics, University of California, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 107:9656-9. 2010
  10. ncbi dictyBase update 2011: web 2.0 functionality and the initial steps towards a genome portal for the Amoebozoa
    Pascale Gaudet
    dictyBase, Northwestern University Biomedical Informatics Center and Center for Genetic Medicine, 420 E Superior St, Chicago, IL 60611, USA
    Nucleic Acids Res 39:D620-4. 2011

Research Grants

  1. Cellular and Biochemical Studies of Myosin Assembly in Dictyostelium
    Thomas Egelhoff; Fiscal Year: 2009
  2. Actions of the Sodium-Hydrogen Exchanger Subtype, NHE1
    Diane L Barber; Fiscal Year: 2010
  3. Quantitative Analysis of Chemotactic Motility Cycle of Ameboid Cells
    JUAN CARLOS contact LASHERAS; Fiscal Year: 2010
  4. CYTOPLASMIC FUCOSYLATION
    Christopher West; Fiscal Year: 1993
  5. INTRACELLULAR ROLE OF CLATHRIN AND ITS REGULATION
    THERESA O HALLORAN; Fiscal Year: 2007
  6. MYOSIN MOVEMENT IN VITRO--MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 1999
  7. Actions of the Sodium-Hydrogen Exchanger Subtype, NHE1
    DIANE BARBER; Fiscal Year: 2007
  8. ROLE OF MDR-RELATED GENES IN DICTYOSTELIUM DEVELOPMENT
    Adam Kuspa; Fiscal Year: 1999
  9. Spatial regulation of cytoskeleton during chemotaxis
    Chang Y Chung; Fiscal Year: 2010
  10. Spatial regulation of cytoskeleton during chemotaxis
    Chang Y Chung; Fiscal Year: 2010

Detail Information

Publications288 found, 100 shown here

  1. ncbi Cells navigate with a local-excitation, global-inhibition-biased excitable network
    Yuan Xiong
    Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 107:17079-86. 2010
    ..Our study brings together heretofore diverse observations on spontaneous cytoskeletal activity, signaling responses to temporal stimuli, and spatial gradient sensing into a unified scheme...
  2. ncbi Legionella eukaryotic-like type IV substrates interfere with organelle trafficking
    Karim Suwwan de Felipe
    Integrated Program in Cellular, Molecular, and Biophysical Studies, Columbia University Medical Center, New York, New York, United States of America
    PLoS Pathog 4:e1000117. 2008
    ..of many endosome-like structures containing undigested material when expressed in the protozoan host Dictyostelium discoideum...
  3. ncbi The ordered extension of pseudopodia by amoeboid cells in the absence of external cues
    Leonard Bosgraaf
    Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
    PLoS ONE 4:e5253. 2009
    ..movement by analyzing the direction, size and timing of approximately 2000 pseudopodia that are extended by Dictyostelium cells...
  4. ncbi Chemotaxis in the absence of PIP3 gradients
    Oliver Hoeller
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Curr Biol 17:813-7. 2007
    Chemotaxing neutrophils and Dictyostelium amoebae produce in their plasma membranes the signaling lipid PI(3,4,5)P3 (PIP3) in gradients, which are orientated with the external chemotactic gradient and have been proposed to act as an ..
  5. ncbi Spatio-temporal analysis of eukaryotic cell motility by improved force cytometry
    Juan C Del Alamo
    Department of Mechanical and Aerospace Engineering, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 104:13343-8. 2007
    ..method and apply it to the analysis of the dynamics of the chemotactic migration of the amoeboid form of Dictyostelium discoideum...
  6. ncbi Molecular mimicry by an F-box effector of Legionella pneumophila hijacks a conserved polyubiquitination machinery within macrophages and protozoa
    Christopher T Price
    Department of Microbiology and Immunology, College of Medicine, University of Louisville, Kentucky, USA
    PLoS Pathog 5:e1000704. 2009
    ..rapid acquisition of polyubiquitinated proteins by the Legionella-containing vacuole (LCV) within macrophages, Dictyostelium discoideum, and Acanthamoeba...
  7. ncbi Attenuation of phospholipid signaling provides a novel mechanism for the action of valproic acid
    Xuehua Xu
    School of Biological Sciences, Royal Holloway University of London, Egham, United Kingdom
    Eukaryot Cell 6:899-906. 2007
    ..Using the social amoeba Dictyostelium discoideum, we show that an acute effect of VPA is the inhibition of chemotactic cell movement, a process ..
  8. ncbi Transient localized patterns in noise-driven reaction-diffusion systems
    Inbal Hecht
    Center for Theoretical Biological Physics, University of California San Diego, La Jolla, California 92093 0319, USA
    Phys Rev Lett 104:158301. 2010
    ..Our work is motivated by a recent biological example showing transiently excited cell membrane regions...
  9. ncbi External and internal constraints on eukaryotic chemotaxis
    Danny Fuller
    Cell and Developmental Biology, Division of Biological Sciences, Department of Physics, and Center for Theoretical Biological Physics, University of California, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 107:9656-9. 2010
    ..Here we determine the chemotactic response of Dictyostelium cells to exponential gradients of varying steepness and local concentration of the chemoattractant cAMP...
  10. ncbi dictyBase update 2011: web 2.0 functionality and the initial steps towards a genome portal for the Amoebozoa
    Pascale Gaudet
    dictyBase, Northwestern University Biomedical Informatics Center and Center for Genetic Medicine, 420 E Superior St, Chicago, IL 60611, USA
    Nucleic Acids Res 39:D620-4. 2011
    dictyBase (http://www.dictybase.org), the model organism database for Dictyostelium, aims to provide the broad biomedical research community with well integrated, high quality data and tools for Dictyostelium discoideum and related ..
  11. ncbi Persistent cell motion in the absence of external signals: a search strategy for eukaryotic cells
    Liang Li
    Department of Physics, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 3:e2093. 2008
    ..cells have a search strategy when they are beyond the range of the signal they would otherwise move toward? Here we ask if single, isolated, Dictyostelium and Polysphondylium amoebae bias their motion in the absence of external cues.
  12. ncbi Infection by tubercular mycobacteria is spread by nonlytic ejection from their amoeba hosts
    Monica Hagedorn
    Departement de Biochimie, Faculte des Sciences, Universite de Geneve, Sciences II, 30 Quai Ernest Ansermet, CH 1211 Geneve 4, Switzerland
    Science 323:1729-33. 2009
    ..Using the social amoeba Dictyostelium as a genetically tractable host for pathogenic mycobacteria, we discovered that M. tuberculosis and M...
  13. ncbi Chemotaxis in shallow gradients is mediated independently of PtdIns 3-kinase by biased choices between random protrusions
    Natalie Andrew
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Nat Cell Biol 9:193-200. 2007
    ..multiple cell types are usually generated when existing ones bifurcate and are rarely made de novo; second, in Dictyostelium cells in shallow chemoattractant gradients, pseudopods are made at the same rate whether cells are moving up ..
  14. ncbi Self-organization of the phosphatidylinositol lipids signaling system for random cell migration
    Yoshiyuki Arai
    Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 107:12399-404. 2010
    ..waves of PtdIns 3,4,5-trisphosphate [PtdIns(3,4,5)P(3)] are generated spontaneously on the membrane of Dictyostelium cells in the absence of a chemoattractant...
  15. ncbi Eukaryotic chemotaxis: a network of signaling pathways controls motility, directional sensing, and polarity
    Kristen F Swaney
    Department of Cell Biology, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA
    Annu Rev Biophys 39:265-89. 2010
    ..By exploiting the genetic advantages of Dictyostelium, investigators are working out the complex network of interactions between the proteins that have been ..
  16. ncbi Genetic control of lithium sensitivity and regulation of inositol biosynthetic genes
    Jason King
    School of Biosciences, Cardiff University, Cardiff, UK
    PLoS ONE 5:e11151. 2010
    ..An alternative approach is to investigate the genetics of Li(+) sensitivity. Using the social amoeba Dictyostelium, we previously identified prolyl oligopeptidase (PO) as a modulator of Li(+) sensitivity...
  17. ncbi Dictyostelium discoideum chemotaxis: threshold for directed motion
    Loling Song
    Laboratory of Atomic and Solid State Physics, Department of Physics, Cornell University, Ithaca, NY 14853, USA
    Eur J Cell Biol 85:981-9. 2006
    The chemotactic response of Dictyostelium discoideum cells to stationary, linear gradients of cyclic adenosine 3',5'-monophosphate (cAMP) was studied using microfluidic devices...
  18. ncbi Phosphoinositides differentially regulate bacterial uptake and Nramp1-induced resistance to Legionella infection in Dictyostelium
    Barbara Peracino
    Department of Clinical and Biological Sciences, University of Turin, AOU S Luigi, Reg Gonzole 10, 10043 Orbassano, Torino, Italy
    J Cell Sci 123:4039-51. 2010
    ..of PI3K, PTEN or PLC on Escherichia coli and Legionella pneumophila uptake by the professional phagocyte Dictyostelium discoideum. We show that L...
  19. ncbi The neuroprotective agent, valproic acid, regulates the mitogen-activated protein kinase pathway through modulation of protein kinase A signalling in Dictyostelium discoideum
    Katrina Boeckeler
    Department of Biology and the Wolfson Institute for Biomedical Research, University College London, London WC1E 6BT, UK
    Eur J Cell Biol 85:1047-57. 2006
    ..To investigate the molecular basis of this effect, we have used the biomedical model system Dictyostelium discoideum to dissect this signalling pathway...
  20. ncbi Conserved developmental transcriptomes in evolutionarily divergent species
    Anup Parikh
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Genome Biol 11:R35. 2010
    ..The social amoebae Dictyostelium discoideum and Dictyostelium purpureum have similar developmental morphologies although their genomes are as ..
  21. ncbi Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system
    Stefan Pukatzki
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:1528-33. 2006
    ..Using Dictyostelium discoideum as a model host, we have identified a virulence mechanism in a non-O1/non-O139 V...
  22. ncbi A common mechanism of action for three mood-stabilizing drugs
    Robin S B Williams
    Intracellular Signalling Group, MRC Laboratory for Molecular Cell Biology, University College London, Gower St, London WC1E 6BT, UK
    Nature 417:292-5. 2002
    ..Moreover, the development of Dictyostelium is sensitive to lithium and to valproic acid, but resistance to both is conferred by deletion of the gene that ..
  23. ncbi Myosin II is essential for the spatiotemporal organization of traction forces during cell motility
    Ruedi Meili
    Section of Cell and Developmental Biology, Division of Biological Sciences, Department of Mechanical and Aerospace Engineering, and Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 21:405-17. 2010
    ..However, it does not reduce significantly the magnitude of the traction forces, uncovering a non-MyoII-mediated mechanism for the contractility of the cell...
  24. ncbi Functional characterization of intracellular Dictyostelium discoideum P2X receptors
    Melanie J Ludlow
    Department of Cell Physiology and Pharmacology, University of Leicester, Leicester LE1 9HN, United Kingdom
    J Biol Chem 284:35227-39. 2009
    ..ion channel activation, extracellular ATP evokes a rapid and transient calcium influx in the model eukaryote Dictyostelium discoideum. Five P2X-like proteins (dP2XA-E) are present in this organism...
  25. ncbi Quantifying in vivo phosphoinositide turnover in chemotactically competent Dictyostelium cells
    Nadine Pawolleck
    Bourne Laboratory, Biomedical Sciences Division, School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, UK
    Methods Mol Biol 571:283-90. 2009
    Phosphoinositide (PI) signalling is one of multiple signalling cascades involved in chemotaxis in Dictyostelium discoideum...
  26. ncbi Chemotaxis: finding the way forward with Dictyostelium
    Jason S King
    CRUK Beatson Institute, Bearsden, Glasgow, UK
    Trends Cell Biol 19:523-30. 2009
    ..This has led to the popularity of Dictyostelium discoideum, with its experimentally-friendly lifestyle and small, haploid genome, as a tool to dissect the ..
  27. ncbi Autophosphorylation activates Dictyostelium myosin II heavy chain kinase A by providing a ligand for an allosteric binding site in the alpha-kinase domain
    Scott W Crawley
    Department of Biochemistry, Queen s University, Kingston, Ontario K7L 3N6, Canada
    J Biol Chem 286:2607-16. 2011
    b>Dictyostelium discoideum myosin II heavy chain kinase A (MHCK A), a member of the atypical α-kinase family, phosphorylates sites in the myosin II tail that block filament assembly...
  28. ncbi Loss of Dictyostelium ATG9 results in a pleiotropic phenotype affecting growth, development, phagocytosis and clearance and replication of Legionella pneumophila
    Sze Man Tung
    Zentrum für Biochemie, Universitat zu Koln, Germany
    Cell Microbiol 12:765-80. 2010
    Infection of Dictyostelium discoideum with Legionella pneumophila resulted in a large number of differentially regulated genes among them three core autophagy genes, ATG8, ATG9 and ATG16...
  29. ncbi Navigation of chemotactic cells by parallel signaling to pseudopod persistence and orientation
    Leonard Bosgraaf
    Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
    PLoS ONE 4:e6842. 2009
    ..Here we use a new computational algorithm to analyze the extension of pseudopods by Dictyostelium cells...
  30. ncbi WASP family proteins: their evolution and its physiological implications
    Douwe M Veltman
    Beatson Institute for Cancer Research, Glasgow, United Kingdom
    Mol Biol Cell 21:2880-93. 2010
    ..An unusual Dictyostelium WASP homologue that has lost the WH1 domain has retained its function in clathrin-mediated endocytosis, ..
  31. ncbi Macroautophagy is dispensable for intracellular replication of Legionella pneumophila in Dictyostelium discoideum
    Grant P Otto
    Department of Anatomy and Cell Biology, Columbia University, 630 West 168th St, New York, NY 10032, USA
    Mol Microbiol 51:63-72. 2004
    ..pneumophila infection requires macroautophagy. As L. pneumophila replicates in Dictyostelium discoideum, and macroautophagy genes have been identified and mutated in D...
  32. ncbi Exploring the control circuit of cell migration by mathematical modeling
    Javier Satulovsky
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Biophys J 94:3671-83. 2008
    ..we show that the mechanism of local stimulation global inhibition can readily account for the behavior of Dictyostelium under a large collection of conditions...
  33. ncbi The novel tyrosine kinase ZAK1 activates GSK3 to direct cell fate specification
    L Kim
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 99:399-408. 1999
    ..Inhibition of GSK3 by 7-TM Wnt/wg receptor signaling is critical for specifying embryonic cell fate patterns. In Dictyostelium, the 7-TM cAMP receptors regulate GSK3 by parallel, antagonistic pathways to establish a developmental body ..
  34. ncbi Ordered patterns of cell shape and orientational correlation during spontaneous cell migration
    Yusuke T Maeda
    Department of Physics, Graduate School of Science, The University of Tokyo, Tokyo, Japan
    PLoS ONE 3:e3734. 2008
    ..Yet little is known about how cells change their own shape and how cells coordinate the deformation and movement. Here, we investigated the mechanism of spontaneous cell migration by using computational analyses...
  35. ncbi The mood stabiliser lithium suppresses PIP3 signalling in Dictyostelium and human cells
    Jason S King
    Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, UK
    Dis Model Mech 2:306-12. 2009
    ..By using the genetically tractable organism Dictyostelium, we show that lithium suppresses PIP(3)-mediated signalling...
  36. ncbi How human leukocytes track down and destroy pathogens: lessons learned from the model organism Dictyostelium discoideum
    Tian Jin
    Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, 12331 Parklawn Drive, Rockville, MD 20852, USA
    Immunol Res 43:118-27. 2009
    ..Interestingly, a soil amoeba, Dictyostelium discoideum, uses these same behaviors to pursue and injest its bacterial food source and to organize its multi-..
  37. ncbi Functional analysis of spontaneous cell movement under different physiological conditions
    Hiroaki Takagi
    Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
    PLoS ONE 3:e2648. 2008
    ..For that purpose, we observed a series of spontaneous movements by Dictyostelium cells at different developmental periods by using a single cell tracking system...
  38. ncbi Macroautophagy is required for multicellular development of the social amoeba Dictyostelium discoideum
    Grant P Otto
    Department of Anatomy and Cell Biology, Columbia University, New York, New York 10032, USA
    J Biol Chem 278:17636-45. 2003
    ..In Dictyostelium, development is induced by starvation, conditions under which autophagy is required for survival in yeast and ..
  39. ncbi Myosin II-independent cytokinesis in Dictyostelium: its mechanism and implications
    T Q Uyeda
    Biomolecular Research Group, National Institute for Advanced Interdisciplinary Research, Tsukuba, Ibaraki, Japan
    Cell Struct Funct 25:1-10. 2000
    Similar to higher animal cells, ameba cells of the cellular slime mold Dictyostelium discoideum form contractile rings containing filaments of myosin II during mitosis, and it is generally believed that contraction of these rings bisects ..
  40. ncbi Molecular dynamics and forces of a motile cell simultaneously visualized by TIRF and force microscopies
    Yoshiaki Iwadate
    Department of Functional Molecular Biology, Graduate School of Medicine, Yamaguchi University, Yamaguchi, Japan
    Biotechniques 44:739-50. 2008
    ..detection of the spatiotemporal distribution of traction forces produced by individual filopodia in migrating Dictyostelium cells, as well as simultaneous visualization of these traction forces and the dynamics of filamentous myosin II...
  41. ncbi Diverse cytopathologies in mitochondrial disease are caused by AMP-activated protein kinase signaling
    Paul B Bokko
    Department of Microbiology, La Trobe University, Melbourne, Victoria 3086, Australia
    Mol Biol Cell 18:1874-86. 2007
    ..a pharmacological AMPK activator in mammalian cells, mimics mitochondrial disease in impairing Dictyostelium phototaxis and that AMPKalpha antisense-inhibited cells were resistant to this effect...
  42. ncbi Formation of Hirano bodies in Dictyostelium and mammalian cells induced by expression of a modified form of an actin-crosslinking protein
    Andrew G Maselli
    Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    J Cell Sci 115:1939-49. 2002
    ..activated actin binding and calcium-insensitive actin filament crosslinking activity were expressed in Dictyostelium and mammalian cells to assess the behavior of these modified forms in vivo...
  43. ncbi Functional plasticity of CH domains
    Mario Gimona
    Department of Cell Biology, Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg, Austria
    FEBS Lett 513:98-106. 2002
    ....
  44. ncbi Adhesion-dependent and contractile ring-independent equatorial furrowing during cytokinesis in mammalian cells
    Masamitsu Kanada
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan
    Mol Biol Cell 16:3865-72. 2005
    ..Nonetheless, myosin II-null cells of the cellular slime mold Dictyostelium divide efficiently when adhering to substrates by making use of polar traction forces...
  45. ncbi Identifying an uptake mechanism for the antiepileptic and bipolar disorder treatment valproic acid using the simple biomedical model Dictyostelium
    Nicole Terbach
    Centre for Biomedical Sciences, School of Biological Sciences, Royal Holloway University of London, Egham TW200EX, UK
    J Cell Sci 124:2267-76. 2011
    ..of cellular, molecular and genetic approaches to characterize VPA uptake using a simple biomedical model, Dictyostelium discoideum. We show that VPA is taken up against an electrochemical gradient in a dose-dependent manner...
  46. ncbi Changing directions in the study of chemotaxis
    Robert R Kay
    MRC Laboratory of Molecular Biology, Hill Road, Cambridge CB2 0QH, UK
    Nat Rev Mol Cell Biol 9:455-63. 2008
    ....
  47. ncbi Xpf and not the Fanconi anaemia proteins or Rev3 accounts for the extreme resistance to cisplatin in Dictyostelium discoideum
    Xiao Yin Zhang
    Medical Research Council, Laboratory for Molecular Biology, Cambridge, United Kingdom
    PLoS Genet 5:e1000645. 2009
    Organisms like Dictyostelium discoideum, often referred to as DNA damage "extremophiles", can survive exposure to extremely high doses of radiation and DNA crosslinking agents...
  48. ncbi Glycogen synthase kinase 3 regulates cell fate in Dictyostelium
    A J Harwood
    Medical Research Council, Laboratory of Molecular Biology, Cambridge, England
    Cell 80:139-48. 1995
    Extracellular cyclic AMP (cAMP) induces the formation of prespore cells in Dictyostelium but inhibits stalk cell formation...
  49. ncbi Cell speed, persistence and information transmission during signal relay and collective migration
    Colin P McCann
    Department of Physics, University of Maryland College Park, College Park, MD 20742 4111, USA
    J Cell Sci 123:1724-31. 2010
    Collective migration is a key feature of the social amoebae Dictyostelium discoideum, where the binding of chemoattractants leads to the production and secretion of additional chemoattractant and the relay of the signal to neighboring ..
  50. ncbi The role of cell contraction and adhesion in dictyostelium motility
    Mathias Buenemann
    Center for Theoretical Biological Physics, University of California, San Diego, La Jolla, California, USA
    Biophys J 99:50-8. 2010
    The crawling motion of Dictyostelium discoideum on substrata involves a number of coordinated events including cell contractions and cell protrusions...
  51. ncbi Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum
    Sarah T Miyata
    Department of Medical Microbiology and Immunology, 1 63 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Infect Immun 79:2941-9. 2011
    ..We present VasX as a novel virulence factor of the T6SS, as a V. cholerae mutant lacking vasX exhibits a phenotype of attenuated virulence toward Dictyostelium discoideum.
  52. ncbi Dictyostelium myosin-IE is a fast molecular motor involved in phagocytosis
    Ulrike Dürrwang
    Abteilung Biophysik, Max Planck Institut fur medizinische Forschung, Jahnstr 29, 69120 Heidelberg, Germany
    J Cell Sci 119:550-8. 2006
    ..A complete analysis of the kinetic and motor properties of Dictyostelium discoideum myosin-IE was achieved by the use of motor domain constructs with artificial lever arms...
  53. ncbi Receptor-dependent and tyrosine phosphatase-mediated inhibition of GSK3 regulates cell fate choice
    Leung Kim
    Laboratory of Cellular and Developmental Biology, Building 50/3351, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 3:523-32. 2002
    Asymmetric body axis formation is central to metazoan development. Dictyostelium establishes an anterior/posterior axis utilizing seven-transmembrane cAMP morphogen receptors (CARs) and GSK3-mediated signal transductions that has a ..
  54. ncbi Infection in a dish: high-throughput analyses of bacterial pathogenesis
    C Leopold Kurz
    Centre d immunologie de Marseille Luminy, Universite de la Mediterranee, Case 906, 13288 Marseille Cedex 9, France
    Curr Opin Microbiol 10:10-6. 2007
    Diverse aspects of host-pathogen interactions have been studied using non-mammalian hosts such as Dictyostelium discoideum, Caenorhabditis elegans, Drosophila melanogaster and Danio rerio for more than 20 years...
  55. ncbi Cross-induction of cell types in Dictyostelium: evidence that DIF-1 is made by prespore cells
    R R Kay
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Development 128:4959-66. 2001
    To investigate how cell type proportions are regulated during Dictyostelium development, we have attempted to find out which cell type produces DIF-1, a diffusible signal molecule inducing the differentiation of prestalk-O cells...
  56. ncbi A coat of filamentous actin prevents clustering of late-endosomal vacuoles in vivo
    Anja Drengk
    Abteilung Zellbiologie and Center for Interdisciplinary Nanostructure Science and Technology CINSaT, Universitat Kassel, Heinrich Plett Str 40, 34132, Kassel, Germany
    Curr Biol 13:1814-9. 2003
    ..Here, we investigate the function of the actin coat that surrounds late endosomes in Dictyostelium. Latrunculin treatment leads to aggregation of these endosomes into grape-like clusters and completely blocks ..
  57. ncbi The inositol polyphosphate 5-phosphatase OCRL1 restricts intracellular growth of Legionella, localizes to the replicative vacuole and binds to the bacterial effector LpnE
    Stefan S Weber
    Institute of Microbiology, ETH Zurich, Zurich, Switzerland
    Cell Microbiol 11:442-60. 2009
    ..pneumophila infections. Bacterial replication and LCV formation occurred more efficiently in Dictyostelium discoideum amoebae lacking the IP5P Dd5P4, a homologue of human OCRL1 (Oculocerebrorenal syndrome of Lowe), ..
  58. ncbi A new set of small, extrachromosomal expression vectors for Dictyostelium discoideum
    Douwe M Veltman
    Cell Biochemistry, Department of Biology, University of Groningen, Haren, The Netherlands
    Plasmid 61:110-8. 2009
    A new set of extrachromosomal Dictyostelium expression vectors is presented that can be modified according to the experimental needs with minimal cloning efforts...
  59. ncbi Key role of local regulation in chemosensing revealed by a new molecular interaction-based modeling method
    Martin Meier-Schellersheim
    Lymphocyte Biology Section and Program in Systems Immunology and Infectious Disease Modeling, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    PLoS Comput Biol 2:e82. 2006
    ..the response to the attractant cyclic adenosine monophosphate (cAMP), made nontrivial predictions about Dictyostelium chemosensing...
  60. ncbi DIF-1 induces the basal disc of the Dictyostelium fruiting body
    Tamao Saito
    Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060 0810, Japan
    Dev Biol 317:444-53. 2008
    The polyketide DIF-1 induces Dictyostelium amoebae to form stalk cells in culture...
  61. ncbi Overexpression of the multidrug efflux pump SmeDEF impairs Stenotrophomonas maltophilia physiology
    Ana Alonso
    , , CSIC, , Cantoblanco, 28049-Madrid
    J Antimicrob Chemother 53:432-4. 2004
    ..maltophilia metabolism. Using a Dictyostelium discoideum model of bacterial virulence, we found overexpression of SmeDEF to be associated with a reduction ..
  62. ncbi YakA, a protein kinase required for the transition from growth to development in Dictyostelium
    G M Souza
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    Development 125:2291-302. 1998
    When Dictyostelium cells starve they arrest their growth and induce the expression of genes necessary for development...
  63. ncbi Exosomes--vesicular carriers for intercellular communication
    Mikael Simons
    Max Planck Institute for Experimental Medicine, Gottingen, Germany
    Curr Opin Cell Biol 21:575-81. 2009
    ..Only recently light have been shed on the subcellular compartments and mechanisms involved in their biogenesis and secretion opening new avenues to understand their functions...
  64. ncbi Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin
    Stefan Pukatzki
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:15508-13. 2007
    ....
  65. ncbi A bZIP/bRLZ transcription factor required for DIF signaling in Dictyostelium
    Christopher R L Thompson
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Development 131:513-23. 2004
    The intermingled differentiation and sorting out of Dictyostelium prestalk-O and prespore cells requires the diffusible signaling molecule DIF-1, and provides an example of a spatial information-independent patterning mechanism...
  66. ncbi Pseudomonas aeruginosa virulence analyzed in a Dictyostelium discoideum host system
    Pierre Cosson
    , , , CH-1211 Geneva 4, Switzerland
    J Bacteriol 184:3027-33. 2002
    ..In this study, we analyzed whether the amoeba Dictyostelium discoideum can be used as a simple model system to analyze the virulence of P. aeruginosa strains...
  67. ncbi Acidocalcisomes are functionally linked to the contractile vacuole of Dictyostelium discoideum
    Norma Marchesini
    Laboratory of Molecular Parasitology, Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 277:8146-53. 2002
    The mass-dense granules of Dictyostelium discoideum were shown to contain large amounts of phosphorus, magnesium, and calcium, as determined by x-ray microanalysis, either in situ or when purified using iodixanol gradient centrifugation...
  68. ncbi Variation, sex, and social cooperation: molecular population genetics of the social amoeba Dictyostelium discoideum
    Jonathan M Flowers
    Department of Biology and Center for Genomics and Systems Biology, New York University, New York, New York, United States of America
    PLoS Genet 6:e1001013. 2010
    b>Dictyostelium discoideum is a eukaryotic microbial model system for multicellular development, cell-cell signaling, and social behavior...
  69. ncbi Dictyostelium possesses highly diverged presenilin/gamma-secretase that regulates growth and cell-fate specification and can accurately process human APP: a system for functional studies of the presenilin/gamma-secretase complex
    Vanessa C McMains
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Dis Model Mech 3:581-94. 2010
    ..We have identified highly diverged orthologs for each gamma-secretase component in the ancient eukaryote Dictyostelium, which lacks equivalents of APP, Notch and other characterized PS/gamma-secretase substrates...
  70. ncbi Cellulose-binding modules from extracellular matrix proteins of Dictyostelium discoideum stalk and sheath
    Y Wang
    Department of Biological Sciences, Macquarie University, Sydney, NSW, Australia
    Eur J Biochem 268:4334-45. 2001
    Cellulose-binding modules (CBMs) of two extracellular matrix proteins, St15 and ShD, from the slime mold Dictyostelium discoideum were expressed in Escherichia coli...
  71. ncbi Vacuolin, a flotillin/reggie-related protein from Dictyostelium oligomerizes for endosome association
    Dirk Wienke
    Laboratory for Molecular Cell Biology, University College London, London, UK
    Eur J Cell Biol 85:991-1000. 2006
    We have analysed the domain structure of vacuolin, a Dictyostelium protein binding to the cytoplasmic surface of late endosomes...
  72. ncbi Influx of extracellular Ca2+ is necessary for electrotaxis in Dictyostelium
    Lynne J Shanley
    School of Medical Sciences, University of Aberdeen, Aberdeen, UK
    J Cell Sci 119:4741-8. 2006
    ..Ca2+](i)) is a pivotal signalling element in cell migration and is thought to be required for chemotaxis of Dictyostelium. Ca2+ signalling may also be important for electrotaxis. However this suggestion has been controversial...
  73. ncbi Intracellular replication of Mycobacterium marinum within Dictyostelium discoideum: efficient replication in the absence of host coronin
    Jonathan M Solomon
    Howard Hughes Medical Institute, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Infect Immun 71:3578-86. 2003
    ..We demonstrate here that M. marinum grows within Dictyostelium discoideum cells, allowing the genetic analysis of host factors that may modulate the replication of ..
  74. ncbi Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP
    Sonya Bader
    Department of Molecular Cell Biology, University of Groningen, Kerklaan 30, 9751NN, Haren, The Netherlands
    Biochem J 402:153-61. 2007
    The Dictyostelium discoideum genome uncovers seven cyclic nucleotide PDEs (phosphodiesterases), of which six have been characterized previously and the seventh is characterized in the present paper...
  75. ncbi Curvature recognition and force generation in phagocytosis
    Margaret Clarke
    Oklahoma Medical Research Foundation, Oklahoma City, OK 73121, USA
    BMC Biol 8:154. 2010
    ..We survey mechanisms that enable a phagocyte to remodel actin organization in response to particles of complex shape...
  76. ncbi Differentiation-inducing-factor dechlorinase, a novel cytosolic dechlorinating enzyme from Dictyostelium discoideum
    O Nayler
    MRC Laboratory of Molecular Biology, Cambridge, England
    Eur J Biochem 208:531-6. 1992
    ..1 (DIF-1) is a dichlorinated alkyl phenone (1-[(3,5-dichloro-2,6-dihydroxy-4-methoxy)phenyl]hexan-1-one) from Dictyostelium discoideum, that induces amoebae to differentiate into stalk cells...
  77. ncbi The Shwachman-Bodian-Diamond syndrome gene encodes an RNA-binding protein that localizes to the pseudopod of Dictyostelium amoebae during chemotaxis
    Deborah Wessels
    W M Keck Dynamic Image Analysis Facility, Department of Biological Sciences, The University of Iowa, Iowa City, IA 52242, USA
    J Cell Sci 119:370-9. 2006
    ..We considered using the social amoeba Dictyostelium discoideum, a model for PMN chemotaxis, an excellent system for elucidating the function of the SBDS protein...
  78. ncbi Glycogen synthase kinase-3 enhances nuclear export of a Dictyostelium STAT protein
    R S Ginger
    Department of Anatomy and Physiology, MSI WTB complex, University of Dundee, Dow Street, Dundee, UK
    EMBO J 19:5483-91. 2000
    Extracellular cAMP stimulates the rapid tyrosine phosphorylation and nuclear translocation of the DICTYOSTELIUM: STAT protein Dd-STATa...
  79. ncbi Actin-based propulsive forces and myosin-II-based contractile forces in migrating Dictyostelium cells
    Yoshiaki Iwadate
    Department of Functional Molecular Biology, Graduate School of Medicine, Yamaguchi University, Yamaguchi 753 8512, Japan
    J Cell Sci 121:1314-24. 2008
    ..of approximately 400 nm, we simultaneously recorded GFP-myosin II dynamics and traction forces under migrating Dictyostelium cells...
  80. ncbi Two novel Src homology 2 domain proteins interact to regulate dictyostelium gene expression during growth and early development
    Christopher Sugden
    School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 285:22927-35. 2010
    There are 13 Dictyostelium Src homology 2 (SH2) domain proteins, almost 10-fold fewer than in mammals, and only three are functionally unassigned...
  81. ncbi Phylogeny, reproductive isolation and kin recognition in the social amoeba Dictyostelium purpureum
    Natasha J Mehdiabadi
    Department of Ecology and Evolutionary Biology, Rice University, Houston, Texas 77005, USA
    Evolution 63:542-8. 2009
    ..b>Dictyostelium, a eukaryotic microbe widely used as a developmental model, has a social fruiting stage in which some formerly ..
  82. ncbi The Dictyostelium type V myosin MyoJ is responsible for the cortical association and motility of contractile vacuole membranes
    Goeh Jung
    Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 186:555-70. 2009
    The contractile vacuole (CV) complex in Dictyostelium is a tubulovesicular osmoregulatory organelle that exhibits extensive motility along the actin-rich cortex, providing a useful model for investigating myosin-dependent membrane ..
  83. ncbi Quantification of social behavior in D. discoideum reveals complex fixed and facultative strategies
    Neil J Buttery
    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Rd, Manchester M13 9PT, UK
    Curr Biol 19:1373-7. 2009
    ..To address these problems, we quantified the social success of naturally occurring genotypes of Dictyostelium discoideum during the formation of chimeric fruiting bodies, consisting of dead stalk cells and viable spores...
  84. ncbi The Dictyostelium bZIP transcription factor DimB regulates prestalk-specific gene expression
    Natasha V Zhukovskaya
    School of Life Sciences, University of Dundee, MSI WTB complex, UK
    Development 133:439-48. 2006
    ..Thus, DIF-1 regulates DimB activity to generate a gradient of ecmA expression in the prestalk zone of the slug...
  85. ncbi Cryptococcus neoformans virulence is enhanced after growth in the genetically malleable host Dictyostelium discoideum
    Judith N Steenbergen
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Infect Immun 71:4862-72. 2003
    ..In this study, we investigated whether Dictyostelium discoideum could serve as an alternate host for C. neoformans. D...
  86. ncbi Dictyostelium morphogenesis
    Cornelis J Weijer
    Division of Cell and Developmental Biology, School of Life Sciences, University of Dundee, Wellcome Trust Biocentre, Dundee DD1 5EH, UK
    Curr Opin Genet Dev 14:392-8. 2004
    During starvation-induced Dictyostelium development, up to several hundred thousand amoeboid cells aggregate, differentiate and form a fruiting body...
  87. ncbi Identification and functional analysis of a developmentally regulated extracellular signal-regulated kinase gene in Dictyostelium discoideum
    C Gaskins
    Department of Biology, University of California, San Diego, La Jolla 92093 0634
    Mol Cell Biol 14:6996-7012. 1994
    ..a developmentally regulated mitogen-activated protein kinase (extracellular signal-regulated kinase) from Dictyostelium discoideum designated ERK1...
  88. ncbi tsunami, the Dictyostelium homolog of the Fused kinase, is required for polarization and chemotaxis
    Linnan Tang
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Genes Dev 22:2278-90. 2008
    In a forward genetic screen for chemotaxis mutants in Dictyostelium discoideum, we identified a loss-of-function mutation, designated tsunami, encoding a homolog of the Fused kinase...
  89. ncbi A protein with similarity to PTEN regulates aggregation territory size by decreasing cyclic AMP pulse size during Dictyostelium discoideum development
    Yitai Tang
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005 1892, USA
    Eukaryot Cell 7:1758-70. 2008
    ..During development, a lawn of Dictyostelium cells breaks up into territories, and within the territories the cells aggregate in dendritic streams to form ..
  90. ncbi Dictyostelium LIS1 is a centrosomal protein required for microtubule/cell cortex interactions, nucleus/centrosome linkage, and actin dynamics
    Markus Rehberg
    A Butenandt Institut Zellbiologie, Ludwig Maximilians Universitat Munchen, D 80336 MUnchen, Germany
    Mol Biol Cell 16:2759-71. 2005
    ..b>Dictyostelium LIS1 (DdLIS1) is a microtubule-associated protein exhibiting 53% identity to human LIS1...
  91. ncbi A novel actin-bundling kinesin-related protein from Dictyostelium discoideum
    Sosuke Iwai
    Department of Life Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan
    J Biol Chem 279:4696-704. 2004
    ..Here, we describe the identification and characterization of a novel kinesin-related protein named DdKin5 from Dictyostelium. DdKin5 consists of an N-terminal conserved motor domain, a central stalk region, and a C-terminal tail domain...
  92. ncbi A novel cytosolic regulator, Pianissimo, is required for chemoattractant receptor and G protein-mediated activation of the 12 transmembrane domain adenylyl cyclase in Dictyostelium
    M Y Chen
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    Genes Dev 11:3218-31. 1997
    ..integration (REMI), we have identified a new gene, Pianissimo (PiaA), involved in cAMP signaling in Dictyostelium discoideum...
  93. ncbi Disruption of aldehyde reductase increases group size in dictyostelium
    Karen Ehrenman
    Howard Hughes Medical Institute and Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005 1892, USA
    J Biol Chem 279:837-47. 2004
    Developing Dictyostelium cells form structures containing approximately 20,000 cells. The size regulation mechanism involves a secreted counting factor (CF) repressing cytosolic glucose levels...
  94. ncbi Inositol pyrophosphates mediate chemotaxis in Dictyostelium via pleckstrin homology domain-PtdIns(3,4,5)P3 interactions
    Hongbo R Luo
    Department of Neuroscience, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA
    Cell 114:559-72. 2003
    ..We demonstrate physiologic regulation of Dictyostelium chemotaxis by InsP7 mediated by its competition with PtdIns(3,4,5)P3 for binding pleckstrin homology (PH) ..
  95. ncbi Cytoskeletal regulation by Dictyostelium Ras subfamily proteins
    Chinten James Lim
    Department of Microbiology and Immunology, University of British Columbia, 300-6174 University Boulevard, Vancouver, British Columbia, Canada V6T 1Z3
    J Muscle Res Cell Motil 23:729-36. 2002
    ..The roles of six of these proteins in regulating actin cytoskeletal functions in Dictyostelium discoideum are discussed in this review.
  96. ncbi Dictyostelium RacH regulates endocytic vesicular trafficking and is required for localization of vacuolin
    Baggavalli P Somesh
    Center for Biochemistry, Medical Faculty, University of Cologne, D 50931 Köln, Germany
    Traffic 7:1194-212. 2006
    b>Dictyostelium RacH localizes predominantly to membranes of the nuclear envelope, endoplasmic reticulum and Golgi apparatus. To investigate the role of this protein, we generated knockout and overexpressor strains...
  97. ncbi A retinoblastoma ortholog controls stalk/spore preference in Dictyostelium
    Harry MacWilliams
    Biozentrum der Ludwig Maximilians Universität, Grosshadernerstrasse 2, 82152 Planegg Martinsried, Germany
    Development 133:1287-97. 2006
    We describe rblA, the Dictyostelium ortholog of the retinoblastoma susceptibility gene Rb. In the growth phase, rblA expression is correlated with several factors that lead to 'preference' for the spore pathway...
  98. ncbi A secondary disruption of the dmpA gene encoding a large membrane protein allows aggregation defective Dictyostelium rasC- cells to form multicellular structures
    Meenal Khosla
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    Dev Biol 292:68-78. 2006
    The disruption of the gene encoding the Dictyostelium Ras subfamily protein, RasC, results in a strain that does not aggregate and has defects in both cAMP signal relay and cAMP chemotaxis...
  99. ncbi A G alpha-dependent pathway that antagonizes multiple chemoattractant responses that regulate directional cell movement
    Joseph A Brzostowski
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive Kidney Diseases, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 18:805-15. 2004
    Chemotactic cells, including neutrophils and Dictyostelium discoideum, orient and move directionally in very shallow chemical gradients...
  100. ncbi RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium
    Leonard Bosgraaf
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Cell Sci 118:1899-910. 2005
    ..Previously, two proteins termed GbpC and GbpD were identified in Dictyostelium, which contain RasGEF and cyclic nucleotide binding domains...
  101. ncbi LrrA, a novel leucine-rich repeat protein involved in cytoskeleton remodeling, is required for multicellular morphogenesis in Dictyostelium discoideum
    Chia I Liu
    Department of Biochemistry, National Cheng Kung University Medical College, Tainan 701, Taiwan, ROC
    Dev Biol 285:238-51. 2005
    ..We have identified a Dictyostelium discoideum gene encoding a novel protein, LrrA, which composes almost entirely leucine-rich repeats (LRRs) ..

Research Grants80

  1. Cellular and Biochemical Studies of Myosin Assembly in Dictyostelium
    Thomas Egelhoff; Fiscal Year: 2009
    ..We are using the simple amoeba Dictyostelium discoideum as a model system for identifying signaling pathways that regulate myosin assembly...
  2. Actions of the Sodium-Hydrogen Exchanger Subtype, NHE1
    Diane L Barber; Fiscal Year: 2010
    ..We showed a leading-edge H+ efflux by NHE1 in mammalian fibroblasts and in Dictyostelium cells is necessary for three stages in cell migration: polarity, actin filament assembly driving membrane ..
  3. Quantitative Analysis of Chemotactic Motility Cycle of Ameboid Cells
    JUAN CARLOS contact LASHERAS; Fiscal Year: 2010
    ..b>Dictyostelium discoideum has proven to be an excellent model for the chemotactic migration of amoeboid cells such as ..
  4. CYTOPLASMIC FUCOSYLATION
    Christopher West; Fiscal Year: 1993
    ..migration, spore coat formation, spore germination, and protein transport rate, in the cellular slime mold Dictyostelium discoideum...
  5. INTRACELLULAR ROLE OF CLATHRIN AND ITS REGULATION
    THERESA O HALLORAN; Fiscal Year: 2007
    ..Our experimental strategy is to use Dictyostelium as a model system...
  6. MYOSIN MOVEMENT IN VITRO--MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 1999
    ..Our demonstrations that we can use Dictyostelium to express large amounts of functional myosin and soluble fragments that contain the motor domain allow us to ..
  7. Actions of the Sodium-Hydrogen Exchanger Subtype, NHE1
    DIANE BARBER; Fiscal Year: 2007
    ..A novel DNHE1 in Drosophila that binds an ERM ortholog, and a novel DdNHE1 in Dictyostelium discoideum that is necessary for cell polarity and chemotaxis were identified, suggesting that actin anchoring ..
  8. ROLE OF MDR-RELATED GENES IN DICTYOSTELIUM DEVELOPMENT
    Adam Kuspa; Fiscal Year: 1999
    ..While the development of Dictyostelium is simpler than that of metazoans, resulting in a ball of spores held on a cellular stalk, proportioning of ..
  9. Spatial regulation of cytoskeleton during chemotaxis
    Chang Y Chung; Fiscal Year: 2010
    ..is necessary for many biological functions, including wound healing, axonal guidance, and the aggregation of Dictyostelium cells. Chemotaxis also plays a role in disease states such as arthritis, cancer, and multiple sclerosis...
  10. Spatial regulation of cytoskeleton during chemotaxis
    Chang Y Chung; Fiscal Year: 2010
    ..is necessary for many biological functions, including wound healing, axonal guidance, and the aggregation of Dictyostelium cells. Chemotaxis also plays a role in disease states such as arthritis, cancer, and multiple sclerosis...
  11. SEQUENCING THE DICTYOSTELIUM GENOME
    Adam Kuspa; Fiscal Year: 2004
    ..b>Dictyostelium is a social amoeba whose study has contributed to our understanding of diverse biological processes common to ..
  12. MESSENGER RNA METABOLISM IN DICTYOSTELIUM AND YEAST
    Allan Jacobson; Fiscal Year: 1992
    ..Therefore, we propose to address the possibility that specific elements (sequences or structures) of unstable Dictyostelium and yeast mRNAs are responsible for the instability of those mRNAs...
  13. MOLECULAR GENETIC ANALYSIS OF MOTILITY: MYOSIN CHAINS
    Rex Chisholm; Fiscal Year: 1990
    ..cDNA clones which encode the Dictyostelium MLCs have recently been isolated...
  14. Cellular and Biochemical Studies of Myosin Assembly in Dictyostelium
    Thomas Egelhoff; Fiscal Year: 2009
    ..We are using the simple amoeba Dictyostelium discoideum as a model system for identifying signaling pathways that regulate myosin assembly...
  15. Molecular Basis for Protein-Phospholipid Interaction
    Jon Lomasney; Fiscal Year: 2005
    ..C (PLC), and the small G protein ARF; as a regulator of the actin cytoskeleton directing cytokinesis in Dictyostelium, and acting as a regulator of endo and exocytosis...
  16. Spatial regulation of cytoskeleton during chemotaxis
    CHANG CHUNG; Fiscal Year: 2007
    ..neutrophils during wound healing, homing of thymocytes, migration of neural crest cells, and aggregation of Dictyostelium. The first step of chemotactic movement is a chemoattractant mediated increase in F-actin polymerization at ..
  17. MOLECULAR ANALYSIS OF CYTOKINESIS IN DICTYOSTELIUM
    Arturo De Lozanne; Fiscal Year: 2006
    ..Toward that goal, three proteins required for cytokinesis in Dictyostelium have been identified...
  18. MOLECULAR ANALYSIS OF CYTOKINESIS IN DICTYOSTELIUM
    Arturo De Lozanne; Fiscal Year: 2003
    ..Toward that goal, three proteins required for cytokinesis in Dictyostelium have been identified...
  19. ROLE OF SIGNAL TRANSDUCTION IN DICTYOSTELIUM DEVELOPMENT
    Peter Devreotes; Fiscal Year: 1992
    ..Recent observations demonstrate that G protein-linked signaling systems are present in microorganisms such as Dictyostelium. Dictyostelium is uniquely suited for studies of chemotaxis in eucaryotic cells and the role of signal ..
  20. CYTOKINESIS IN DICTYOSTELIUM--A MOLECULAR ANALYSIS
    James Spudich; Fiscal Year: 2001
    ..Key to a long term undertaking to solve this problem is the judicious choice of a model system. Dictyostelium discoideum is a model of choice because it's behavioral characteristics during mitosis and cytokinesis are ..