Summary: A genus of protozoa, formerly also considered a fungus. Its natural habitat is decaying forest leaves, where it feeds on bacteria. D. discoideum is the best-known species and is widely used in biomedical research.
Publications343 found, 100 shown here
- Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model systemStefan Pukatzki
Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 103:1528-33. 2006..Using Dictyostelium discoideum as a model host, we have identified a virulence mechanism in a non-O1/non-O139 V...
- dictyBase update 2011: web 2.0 functionality and the initial steps towards a genome portal for the AmoebozoaPascale Gaudet
dictyBase, Northwestern University Biomedical Informatics Center and Center for Genetic Medicine, 420 E Superior St, Chicago, IL 60611, USA
Nucleic Acids Res 39:D620-4. 2011dictyBase (http://www.dictybase.org), the model organism database for Dictyostelium, aims to provide the broad biomedical research community with well integrated, high quality data and tools for Dictyostelium discoideum and related ..
- Phylogeny-wide analysis of social amoeba genomes highlights ancient origins for complex intercellular communicationAndrew J Heidel
Leibniz Institute for Age Research Fritz Lipmann Institute, D 07745 Jena, Germany
Genome Res 21:1882-91. 2011b>Dictyostelium discoideum (DD), an extensively studied model organism for cell and developmental biology, belongs to the most derived group 4 of social amoebas, a clade of altruistic multicellular organisms...
- Legionella eukaryotic-like type IV substrates interfere with organelle traffickingKarim Suwwan de Felipe
Integrated Program in Cellular, Molecular, and Biophysical Studies, Columbia University Medical Center, New York, New York, United States of America
PLoS Pathog 4:e1000117. 2008..of many endosome-like structures containing undigested material when expressed in the protozoan host Dictyostelium discoideum...
- Eukaryotic chemotaxis: a network of signaling pathways controls motility, directional sensing, and polarityKristen F Swaney
Department of Cell Biology, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA
Annu Rev Biophys 39:265-89. 2010..By exploiting the genetic advantages of Dictyostelium, investigators are working out the complex network of interactions between the proteins that have been ..
- The ordered extension of pseudopodia by amoeboid cells in the absence of external cuesLeonard Bosgraaf
Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
PLoS ONE 4:e5253. 2009..movement by analyzing the direction, size and timing of approximately 2000 pseudopodia that are extended by Dictyostelium cells...
- The onset of collective behavior in social amoebaeThomas Gregor
Graduate School of Arts and Sciences, University of Tokyo, Tokyo 153 8902, Japan
Science 328:1021-5. 2010In the social amoebae Dictyostelium discoideum, periodic synthesis and release of extracellular cyclic adenosine 3',5'-monophosphate (cAMP) guide cell aggregation and commitment to form fruiting bodies...
- Comparative genomics of the social amoebae Dictyostelium discoideum and Dictyostelium purpureumRichard Sucgang
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Genome Biol 12:R20. 2011..There are four groups of dictyostelids, with the most derived being a group that contains the model species Dictyostelium discoideum.
- Conserved developmental transcriptomes in evolutionarily divergent speciesAnup Parikh
Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Genome Biol 11:R35. 2010..The social amoebae Dictyostelium discoideum and Dictyostelium purpureum have similar developmental morphologies although their genomes are as ..
- External and internal constraints on eukaryotic chemotaxisDanny Fuller
Cell and Developmental Biology, Division of Biological Sciences, Department of Physics, and Center for Theoretical Biological Physics, University of California, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 107:9656-9. 2010..Here we determine the chemotactic response of Dictyostelium cells to exponential gradients of varying steepness and local concentration of the chemoattractant cAMP...
- Molecular mimicry by an F-box effector of Legionella pneumophila hijacks a conserved polyubiquitination machinery within macrophages and protozoaChristopher T Price
Department of Microbiology and Immunology, College of Medicine, University of Louisville, Kentucky, USA
PLoS Pathog 5:e1000704. 2009..rapid acquisition of polyubiquitinated proteins by the Legionella-containing vacuole (LCV) within macrophages, Dictyostelium discoideum, and Acanthamoeba...
- Transient localized patterns in noise-driven reaction-diffusion systemsInbal Hecht
Center for Theoretical Biological Physics, University of California San Diego, La Jolla, California 92093 0319, USA
Phys Rev Lett 104:158301. 2010..Our work is motivated by a recent biological example showing transiently excited cell membrane regions...
- Spatio-temporal analysis of eukaryotic cell motility by improved force cytometryJuan C Del Alamo
Department of Mechanical and Aerospace Engineering, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 104:13343-8. 2007..method and apply it to the analysis of the dynamics of the chemotactic migration of the amoeboid form of Dictyostelium discoideum...
- Attenuation of phospholipid signaling provides a novel mechanism for the action of valproic acidXuehua Xu
School of Biological Sciences, Royal Holloway University of London, Egham, United Kingdom
Eukaryot Cell 6:899-906. 2007..Using the social amoeba Dictyostelium discoideum, we show that an acute effect of VPA is the inhibition of chemotactic cell movement, a process ..
- Chemotaxis in the absence of PIP3 gradientsOliver Hoeller
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
Curr Biol 17:813-7. 2007Chemotaxing neutrophils and Dictyostelium amoebae produce in their plasma membranes the signaling lipid PI(3,4,5)P3 (PIP3) in gradients, which are orientated with the external chemotactic gradient and have been proposed to act as an ..
- Tumor suppressor PTEN mediates sensing of chemoattractant gradientsMiho Iijima
Department of Cell Biology, Johns Hopkins University, School of Medicine, 21205, Baltimore, MD, USA
Cell 109:599-610. 2002..Disruption of the PI 3-phosphatase, PTEN, in Dictyostelium discoideum dramatically prolonged and broadened the PH domain relocation and actin polymerization responses, ..
- Persistent cell motion in the absence of external signals: a search strategy for eukaryotic cellsLiang Li
Department of Physics, Princeton University, Princeton, New Jersey, United States of America
PLoS ONE 3:e2093. 2008..cells have a search strategy when they are beyond the range of the signal they would otherwise move toward? Here we ask if single, isolated, Dictyostelium and Polysphondylium amoebae bias their motion in the absence of external cues.
- Cells navigate with a local-excitation, global-inhibition-biased excitable networkYuan Xiong
Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
Proc Natl Acad Sci U S A 107:17079-86. 2010..Our study brings together heretofore diverse observations on spontaneous cytoskeletal activity, signaling responses to temporal stimuli, and spatial gradient sensing into a unified scheme...
- Infection by tubercular mycobacteria is spread by nonlytic ejection from their amoeba hostsMonica Hagedorn
Departement de Biochimie, Faculte des Sciences, Universite de Geneve, Sciences II, 30 Quai Ernest Ansermet, CH 1211 Geneve 4, Switzerland
Science 323:1729-33. 2009..Using the social amoeba Dictyostelium as a genetically tractable host for pathogenic mycobacteria, we discovered that M. tuberculosis and M...
- Chemotaxis in shallow gradients is mediated independently of PtdIns 3-kinase by biased choices between random protrusionsNatalie Andrew
School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
Nat Cell Biol 9:193-200. 2007..multiple cell types are usually generated when existing ones bifurcate and are rarely made de novo; second, in Dictyostelium cells in shallow chemoattractant gradients, pseudopods are made at the same rate whether cells are moving up ..
- Single-gene greenbeard effects in the social amoeba Dictyostelium discoideumDavid C Queller
Department of Ecology and Evolution, MS 170, Rice University, Post Office Box 1892, Houston, TX 77251 1892, USA
Science 299:105-6. 2003..The csA gene in Dictyostelium discoideum acts as a single-gene greenbeard...
- Dictyostelium finds new roles to modelJeffrey G Williams
College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
Genetics 185:717-26. 2010..made using the model be of wider relevance? This review addresses these issues for the social amoeba Dictyostelium and highlights some of the organisms more recent applications...
- Genetic control of lithium sensitivity and regulation of inositol biosynthetic genesJason King
School of Biosciences, Cardiff University, Cardiff, UK
PLoS ONE 5:e11151. 2010..An alternative approach is to investigate the genetics of Li(+) sensitivity. Using the social amoeba Dictyostelium, we previously identified prolyl oligopeptidase (PO) as a modulator of Li(+) sensitivity...
- Dictyostelium discoideum chemotaxis: threshold for directed motionLoling Song
Laboratory of Atomic and Solid State Physics, Department of Physics, Cornell University, Ithaca, NY 14853, USA
Eur J Cell Biol 85:981-9. 2006The chemotactic response of Dictyostelium discoideum cells to stationary, linear gradients of cyclic adenosine 3',5'-monophosphate (cAMP) was studied using microfluidic devices...
- The neuroprotective agent, valproic acid, regulates the mitogen-activated protein kinase pathway through modulation of protein kinase A signalling in Dictyostelium discoideumKatrina Boeckeler
Department of Biology and the Wolfson Institute for Biomedical Research, University College London, London WC1E 6BT, UK
Eur J Cell Biol 85:1047-57. 2006..To investigate the molecular basis of this effect, we have used the biomedical model system Dictyostelium discoideum to dissect this signalling pathway...
- Phosphoinositides differentially regulate bacterial uptake and Nramp1-induced resistance to Legionella infection in DictyosteliumBarbara Peracino
Department of Clinical and Biological Sciences, University of Turin, AOU S Luigi, Reg Gonzole 10, 10043 Orbassano, Torino, Italy
J Cell Sci 123:4039-51. 2010..of PI3K, PTEN or PLC on Escherichia coli and Legionella pneumophila uptake by the professional phagocyte Dictyostelium discoideum. We show that L...
- Autophagy genes protect against Salmonella typhimurium infection and mediate insulin signaling-regulated pathogen resistanceKailiang Jia
Department of Internal Medicine, The Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Proc Natl Acad Sci U S A 106:14564-9. 2009..Serovar Typhimurium (Salmonella typhimurium) infection in 2 model organisms, Caenorhabditis elegans and Dictyostelium discoideum...
- Adenylyl cyclase localization regulates streaming during chemotaxisPaul W Kriebel
Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Building 37 Room 1E24, Bethesda, MD 20892, USA
Cell 112:549-60. 2003We studied the role of the adenylyl cyclase ACA in Dictyostelium discoideum chemotaxis and streaming. In this process, cells orient themselves in a head to tail fashion as they are migrating to form aggregates...
- An autoregulatory circuit for long-range self-organization in Dictyostelium cell populationsSatoshi Sawai
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
Nature 433:323-6. 2005Nutrient-deprived Dictyostelium amoebae aggregate to form a multicellular structure by chemotaxis, moving towards propagating waves of cyclic AMP that are relayed from cell to cell...
- Pseudopod growth and evolution during cell movement is controlled through SCAR/WAVE dephosphorylationSeiji Ura
Beatson Institute for Cancer Research, Bearsden, Glasgow, UK
Curr Biol 22:553-61. 2012..SCAR/WAVE is phosphorylated at multiple sites, but how this affects its biological activity is unclear. Here we show that dephosphorylation of Dictyostelium SCAR controls normal pseudopod dynamics.
- Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosisMichael Carnell
Cancer Research UK Beatson Institute for Cancer Research, Bearsden, Glasgow G61 1BD, Scotland, UK
J Cell Biol 193:831-9. 2011..In this paper, we show that WASH coats mature Dictyostelium discoideum lysosomes and is essential for exocytosis of indigestible material...
- The cyclase-associated protein CAP as regulator of cell polarity and cAMP signaling in DictyosteliumAngelika A Noegel
Center for Biochemistry, Medical Faculty, University of Cologne, 50931 Cologne, Germany
Mol Biol Cell 15:934-45. 2004..We show that cell polarization, F-actin organization, and phototaxis are altered in a Dictyostelium CAP knockout mutant...
- Pharmacological profiling of the Dictyostelium adenylate cyclases ACA, ACB and ACGElisa Alvarez-Curto
School of Life Sciences, University of Dundee, Dundee, Scotland, UK
Biochem J 401:309-16. 2007..crucial roles in controlling cell movement and gene regulation throughout development of the social amoeba Dictyostelium discoideum...
- Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideumSarah T Miyata
Department of Medical Microbiology and Immunology, 1 63 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Infect Immun 79:2941-9. 2011..We present VasX as a novel virulence factor of the T6SS, as a V. cholerae mutant lacking vasX exhibits a phenotype of attenuated virulence toward Dictyostelium discoideum.
- Chemotaxis: finding the way forward with DictyosteliumJason S King
CRUK Beatson Institute, Bearsden, Glasgow, UK
Trends Cell Biol 19:523-30. 2009..This has led to the popularity of Dictyostelium discoideum, with its experimentally-friendly lifestyle and small, haploid genome, as a tool to dissect the ..
- Macroautophagy is dispensable for intracellular replication of Legionella pneumophila in Dictyostelium discoideumGrant P Otto
Department of Anatomy and Cell Biology, Columbia University, 630 West 168th St, New York, NY 10032, USA
Mol Microbiol 51:63-72. 2004..pneumophila infection requires macroautophagy. As L. pneumophila replicates in Dictyostelium discoideum, and macroautophagy genes have been identified and mutated in D...
- Quantifying in vivo phosphoinositide turnover in chemotactically competent Dictyostelium cellsNadine Pawolleck
Bourne Laboratory, Biomedical Sciences Division, School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, UK
Methods Mol Biol 571:283-90. 2009Phosphoinositide (PI) signalling is one of multiple signalling cascades involved in chemotaxis in Dictyostelium discoideum...
- Autophosphorylation activates Dictyostelium myosin II heavy chain kinase A by providing a ligand for an allosteric binding site in the alpha-kinase domainScott W Crawley
Department of Biochemistry, Queen s University, Kingston, Ontario K7L 3N6, Canada
J Biol Chem 286:2607-16. 2011b>Dictyostelium discoideum myosin II heavy chain kinase A (MHCK A), a member of the atypical α-kinase family, phosphorylates sites in the myosin II tail that block filament assembly...
- Loss of Dictyostelium ATG9 results in a pleiotropic phenotype affecting growth, development, phagocytosis and clearance and replication of Legionella pneumophilaSze Man Tung
Zentrum für Biochemie, Universitat zu Koln, Germany
Cell Microbiol 12:765-80. 2010Infection of Dictyostelium discoideum with Legionella pneumophila resulted in a large number of differentially regulated genes among them three core autophagy genes, ATG8, ATG9 and ATG16...
- WASP family proteins: their evolution and its physiological implicationsDouwe M Veltman
Beatson Institute for Cancer Research, Glasgow, United Kingdom
Mol Biol Cell 21:2880-93. 2010..An unusual Dictyostelium WASP homologue that has lost the WH1 domain has retained its function in clathrin-mediated endocytosis, ..
- Orientation of chemotactic cells and growth cones: models and mechanismsH Meinhardt
Max Planck Institut fur Entwicklungsbiologie, Spemannstr 35, D 72076 Tubingen, Germany
J Cell Sci 112:2867-74. 1999..Travelling waves or oscillations in counter phase can emerge on the cell cortex, in agreement with observations reported in the literature. In other ranges of parameters, the model accounts for the generation of a stable cell polarity...
- Two complementary, local excitation, global inhibition mechanisms acting in parallel can explain the chemoattractant-induced regulation of PI(3,4,5)P3 response in dictyostelium cellsLan Ma
Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
Biophys J 87:3764-74. 2004Chemotaxing cells, such as Dictyostelium and mammalian neutrophils, sense shallow chemoattractant gradients and respond with highly polarized changes in cell morphology and motility...
- Exploring the control circuit of cell migration by mathematical modelingJavier Satulovsky
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Biophys J 94:3671-83. 2008..we show that the mechanism of local stimulation global inhibition can readily account for the behavior of Dictyostelium under a large collection of conditions...
- Towards a molecular understanding of human diseases using Dictyostelium discoideumRobin S B Williams
Department of Biology and the Wolfson Institute for Biomedical Research, University College London, London, WC1E 6BT, UK
Trends Mol Med 12:415-24. 2006The social amoeba Dictyostelium discoideum is increasingly being used as a simple model for the investigation of problems that are relevant to human health...
- Navigation of chemotactic cells by parallel signaling to pseudopod persistence and orientationLeonard Bosgraaf
Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
PLoS ONE 4:e6842. 2009..Here we use a new computational algorithm to analyze the extension of pseudopods by Dictyostelium cells...
- Self-organization of the phosphatidylinositol lipids signaling system for random cell migrationYoshiyuki Arai
Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
Proc Natl Acad Sci U S A 107:12399-404. 2010..waves of PtdIns 3,4,5-trisphosphate [PtdIns(3,4,5)P(3)] are generated spontaneously on the membrane of Dictyostelium cells in the absence of a chemoattractant...
- Collective cell migration in developmentCornelis J Weijer
Division of Cell and Developmental Biology, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
J Cell Sci 122:3215-23. 2009....
- The mood stabiliser lithium suppresses PIP3 signalling in Dictyostelium and human cellsJason S King
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, UK
Dis Model Mech 2:306-12. 2009..By using the genetically tractable organism Dictyostelium, we show that lithium suppresses PIP(3)-mediated signalling...
- The novel tyrosine kinase ZAK1 activates GSK3 to direct cell fate specificationL Kim
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Cell 99:399-408. 1999..Inhibition of GSK3 by 7-TM Wnt/wg receptor signaling is critical for specifying embryonic cell fate patterns. In Dictyostelium, the 7-TM cAMP receptors regulate GSK3 by parallel, antagonistic pathways to establish a developmental body ..
- Ordered patterns of cell shape and orientational correlation during spontaneous cell migrationYusuke T Maeda
Department of Physics, Graduate School of Science, The University of Tokyo, Tokyo, Japan
PLoS ONE 3:e3734. 2008..Yet little is known about how cells change their own shape and how cells coordinate the deformation and movement. Here, we investigated the mechanism of spontaneous cell migration by using computational analyses...
- How human leukocytes track down and destroy pathogens: lessons learned from the model organism Dictyostelium discoideumTian Jin
Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, 12331 Parklawn Drive, Rockville, MD 20852, USA
Immunol Res 43:118-27. 2009..Interestingly, a soil amoeba, Dictyostelium discoideum, uses these same behaviors to pursue and injest its bacterial food source and to organize its multi-..
- Macroautophagy is required for multicellular development of the social amoeba Dictyostelium discoideumGrant P Otto
Department of Anatomy and Cell Biology, Columbia University, New York, New York 10032, USA
J Biol Chem 278:17636-45. 2003..In Dictyostelium, development is induced by starvation, conditions under which autophagy is required for survival in yeast and ..
- Functional analysis of spontaneous cell movement under different physiological conditionsHiroaki Takagi
Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
PLoS ONE 3:e2648. 2008..For that purpose, we observed a series of spontaneous movements by Dictyostelium cells at different developmental periods by using a single cell tracking system...
- Activated membrane patches guide chemotactic cell motilityInbal Hecht
Center for Theoretical Biological Physics, University of California San Diego, La Jolla, California, United States of America
PLoS Comput Biol 7:e1002044. 2011..how these patches are related to cell motion, we examine the spatial localization of RasGTP in chemotaxing Dictyostelium discoideum cells under conditions where the vertical extent of the cell was restricted...
- Stretching actin filaments within cells enhances their affinity for the myosin II motor domainTaro Q P Uyeda
National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
PLoS ONE 6:e26200. 2011..of actin filaments in vivo, we expressed GFP-fused S1 with mutations that enhanced its affinity for actin in Dictyostelium cells...
- Involvement of the AP-1 adaptor complex in early steps of phagocytosis and macropinocytosisYaya Lefkir
Institut de Biologie et Chimie des Proteines, UMR5086 CNRS Université Lyon I, IFR 128 Biosciences Lyon Gerland, 69367 Lyon, France
Mol Biol Cell 15:861-9. 2004..Here, we show that AP-1 is also localized to phagocytic cups in murine macrophages as well as in Dictyostelium amoebae...
- Leveraging the membrane - cytoskeleton interface with myosin-1Russell E McConnell
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37205, USA
Trends Cell Biol 20:418-26. 2010..These properties put myosins-1 in a unique position to regulate dynamic membrane-cytoskeleton interactions and respond to physical forces during these events...
- Gene discovery in the Acanthamoeba castellanii genomeIain J Anderson
Department of Parasite Genomics, The Institute for Genomic Research, 9712 Medical Center Dr, Rockville, MD 20850, USA
Protist 156:203-14. 2005..The data predict that A. castellanii exhibits a greater biosynthetic capacity than the free-living Dictyostelium discoideum and the parasite Entamoeba histolytica, providing an explanation for the ability of A...
- Overlapping functions of the two talin homologues in DictyosteliumMasatsune Tsujioka
National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
Eukaryot Cell 7:906-16. 2008..The cellular slime mold Dictyostelium discoideum has two talin homologues, talA and talB, and earlier studies have characterized the single knockout ..
- SCAR knockouts in Dictyostelium: WASP assumes SCAR's position and upstream regulators in pseudopodsDouwe M Veltman
Beatson Institute for Cancer Research, Glasgow G61 1BD, Scotland, UK
J Cell Biol 198:501-8. 2012..We show that, unexpectedly, Dictyostelium discoideum SCAR knockouts could still spread, migrate, and chemotax using pseudopods driven by the Arp2/3 ..
- Automated characterization of cell shape changes during amoeboid motility by skeletonizationYuan Xiong
Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
BMC Syst Biol 4:33. 2010....
- A Gβγ effector, ElmoE, transduces GPCR signaling to the actin network during chemotaxisJianshe Yan
Chemotaxis Signal Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
Dev Cell 22:92-103. 2012..Here, we identified a Gβγ effector, ElmoE in Dictyostelium, and demonstrated that it is required for GPCR-mediated chemotaxis...
- MidA is a putative methyltransferase that is required for mitochondrial complex I functionSergio Carilla-Latorre
Instituto de Investigaciones Biomedicas Alberto Sols CSIC UAM, Arturo Duperier 4, 28029 Madrid, Spain
J Cell Sci 123:1674-83. 2010b>Dictyostelium and human MidA are homologous proteins that belong to a family of proteins of unknown function called DUF185...
- An Elmo-like protein associated with myosin II restricts spurious F-actin events to coordinate phagocytosis and chemotaxisNilgun Isik
Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, 12331 Parklawn Drive, Rockville, MD 20852, USA
Dev Cell 15:590-602. 2008..We identified an Elmo-like protein, ElmoA, in Dictyostelium discoideum that unexpectedly functions as a negative regulator of actin polymerization...
- Propagating chemoattractant waves coordinate periodic cell movement in Dictyostelium slugsD Dormann
School of Life Sciences, Division of Cell and Developmental Biology, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK
Development 128:4535-43. 2001Migration and behaviour of Dictyostelium slugs results from coordinated movement of its constituent cells. It has been proposed that cell movement is controlled by propagating waves of cAMP as during aggregation and in the mound...
- Primitive agriculture in a social amoebaDebra A Brock
Department of Ecology and Evolutionary Biology, Rice University, 6100 Main Street, Houston, Texas 77005, USA
Nature 469:393-6. 2011..Here we show that the social amoeba Dictyostelium discoideum has a primitive farming symbiosis that includes dispersal and prudent harvesting of the crop...
- Dictyostelium chemotaxis: essential Ras activation and accessory signalling pathways for amplificationArjan Kortholt
Department of Cell Biochemistry, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands
EMBO Rep 12:1273-9. 2011..We used Dictyostelium mutants to investigate the minimal requirements for chemotaxis, and identified a basal signalling module ..
- A common mechanism of action for three mood-stabilizing drugsRobin S B Williams
Intracellular Signalling Group, MRC Laboratory for Molecular Cell Biology, University College London, Gower St, London WC1E 6BT, UK
Nature 417:292-5. 2002..Moreover, the development of Dictyostelium is sensitive to lithium and to valproic acid, but resistance to both is conferred by deletion of the gene that ..
- The antiepileptic drug valproic acid and other medium-chain fatty acids acutely reduce phosphoinositide levels independently of inositol in DictyosteliumPishan Chang
Centre for Biomedical Sciences, School of Biological Sciences, Royal Holloway University of London, Egham, TW20 0EX, UK
Dis Model Mech 5:115-24. 2012..work identified a previously unknown effect of VPA in reducing phosphoinositide production in the simple model Dictyostelium followed by the transfer of data to a mammalian synaptic release model...
- The in vivo role of annexin VII (synexin): characterization of an annexin VII-deficient Dictyostelium mutant indicates an involvement in Ca(2+)-regulated processesV Doring
Max Planck Institut fur Biochemie, Martinsried, FRG
J Cell Sci 108:2065-76. 1995b>Dictyostelium discoideum cells harbor two annexin VII isoforms of 47 and 51 kDa which are present throughout development...
- Cloning and functional expression of UGT genes encoding sterol glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideumD Warnecke
Universitat Hamburg, Institut fur Allgemeine Botanik, 22609 Hamburg, Germany
J Biol Chem 274:13048-59. 1999..4.1.173). We cloned genes from three different yeasts and from Dictyostelium discoideum, the deduced amino acid sequences of which all showed similarities with plant sterol ..
- Coupling mechanism of a GPCR and a heterotrimeric G protein during chemoattractant gradient sensing in DictyosteliumXuehua Xu
Chemotaxis Signal Section, National Institutes of Health, Rockville, MD 20852, USA
Sci Signal 3:ra71. 2010..We suggest that chemoattractant receptors use a ligand-induced coupling rather than a precoupled mechanism to control the activation of G proteins during chemotaxis...
- Use of a Dictyostelium model for isolation of genetic loci associated with phagocytosis and virulence in Klebsiella pneumoniaeYi Jiun Pan
Department of Microbiology, National Taiwan University College of Medicine, 1, Sec 1, Jen Ai Rd, Taipei 10051, Taiwan
Infect Immun 79:997-1006. 2011Phagocytosis resistance is an important virulence factor in Klebsiella pneumoniae. Dictyostelium has been used to study the interaction between phagocytes and bacteria because of its similarity to mammalian macrophages...
- Regulation of PTEN by Rho small GTPasesZhong Li
Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Nat Cell Biol 7:399-404. 2005..PTEN's intracellular localization and presumably activity are regulated by chemoattractants in Dictyostelium and mouse neutrophils. However, the mechanisms for its regulation remain elusive...
- A role for tRNA(His) guanylyltransferase (Thg1)-like proteins from Dictyostelium discoideum in mitochondrial 5'-tRNA editingMaria G Abad
Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
RNA 17:613-23. 2011..Here we investigate the activities of four Thg1-like proteins (TLPs) encoded by the genome of the slime mold, Dictyostelium discoideum (a member of the eukaryotic supergroup Amoebozoa)...
- A Rap/phosphatidylinositol 3-kinase pathway controls pseudopod formation [corrected]Arjan Kortholt
Department of Molecular Cell Biology, University of Groningen, 9751 NN Haren, The Netherlands
Mol Biol Cell 21:936-45. 2010GbpD, a Dictyostelium discoideum guanine exchange factor specific for Rap1, has been implicated in adhesion, cell polarity, and chemotaxis...
- C-sequence of the Dictyostelium cytoplasmic dynein participates in processivity modulationNaoki Numata
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3 8 1 Komaba, Meguro ku, Tokyo, Japan
FEBS Lett 585:1185-90. 2011We examined the functional roles of C-sequence, a 47-kDa non-AAA+ module at the C-terminal end of the 380-kDa Dictyostelium dynein motor domain...
- Dictyostelium Aurora kinase has properties of both Aurora A and Aurora B kinasesHui Li
Section of Molecular Cell and Developmental Biology, University of Texas at Austin, Austin, TX 78712, USA
Eukaryot Cell 7:894-905. 2008..We show that Dictyostelium discoideum contains a single Aurora kinase, DdAurora, that displays characteristics of both Aurora A and B...
- Differentiation-inducing factor-1 and -2 function also as modulators for Dictyostelium chemotaxisHidekazu Kuwayama
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
PLoS ONE 4:e6658. 2009In the early stages of development of the cellular slime mold Dictyostelium discoideum, chemotaxis toward cAMP plays a pivotal role in organizing discrete cells into a multicellular structure...
- Control of cell polarity and chemotaxis by Akt/PKB and PI3 kinase through the regulation of PAKaC Y Chung
Section of Cell and Developmental Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0634, USA
Mol Cell 7:937-47. 2001..Addition of the PI3K inhibitor LY294002 results in a rapid loss of cell polarity and the axial distribution of actin, myosin, and PAKa. These results provide a mechanism by which PI3K regulates chemotaxis...
- Proteasomes from Dictyostelium discoideum: characterization of structure and functionT M Schauer
Max Planck Institute for Biochemistry, Martinsried, Germany
J Struct Biol 111:135-47. 1993We have isolated and purified 20 S proteasomes from Dictyostelium discoideum and characterized their proteolytic activities. Two-dimensional electrophoresis revealed 13 distinct spots...
- The group migration of Dictyostelium cells is regulated by extracellular chemoattractant degradationGene L Garcia
Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biol Cell 20:3295-304. 2009Starvation of Dictyostelium induces a developmental program in which cells form an aggregate that eventually differentiates into a multicellular structure...
- Glycogen synthase kinase 3 regulates cell fate in DictyosteliumA J Harwood
Medical Research Council, Laboratory of Molecular Biology, Cambridge, England
Cell 80:139-48. 1995Extracellular cyclic AMP (cAMP) induces the formation of prespore cells in Dictyostelium but inhibits stalk cell formation...
- Functional plasticity of CH domainsMario Gimona
Department of Cell Biology, Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg, Austria
FEBS Lett 513:98-106. 2002....
- Molecular dynamics and forces of a motile cell simultaneously visualized by TIRF and force microscopiesYoshiaki Iwadate
Department of Functional Molecular Biology, Graduate School of Medicine, Yamaguchi University, Yamaguchi, Japan
Biotechniques 44:739-50. 2008..detection of the spatiotemporal distribution of traction forces produced by individual filopodia in migrating Dictyostelium cells, as well as simultaneous visualization of these traction forces and the dynamics of filamentous myosin II...
- Changing directions in the study of chemotaxisRobert R Kay
MRC Laboratory of Molecular Biology, Hill Road, Cambridge CB2 0QH, UK
Nat Rev Mol Cell Biol 9:455-63. 2008....
- Xpf and not the Fanconi anaemia proteins or Rev3 accounts for the extreme resistance to cisplatin in Dictyostelium discoideumXiao Yin Zhang
Medical Research Council, Laboratory for Molecular Biology, Cambridge, United Kingdom
PLoS Genet 5:e1000645. 2009Organisms like Dictyostelium discoideum, often referred to as DNA damage "extremophiles", can survive exposure to extremely high doses of radiation and DNA crosslinking agents...
- Diverse cytopathologies in mitochondrial disease are caused by AMP-activated protein kinase signalingPaul B Bokko
Department of Microbiology, La Trobe University, Melbourne, Victoria 3086, Australia
Mol Biol Cell 18:1874-86. 2007..a pharmacological AMPK activator in mammalian cells, mimics mitochondrial disease in impairing Dictyostelium phototaxis and that AMPKalpha antisense-inhibited cells were resistant to this effect...
- Formation of Hirano bodies in Dictyostelium and mammalian cells induced by expression of a modified form of an actin-crosslinking proteinAndrew G Maselli
Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
J Cell Sci 115:1939-49. 2002..activated actin binding and calcium-insensitive actin filament crosslinking activity were expressed in Dictyostelium and mammalian cells to assess the behavior of these modified forms in vivo...
- The role of cell contraction and adhesion in dictyostelium motilityMathias Buenemann
Center for Theoretical Biological Physics, University of California, San Diego, La Jolla, California, USA
Biophys J 99:50-8. 2010The crawling motion of Dictyostelium discoideum on substrata involves a number of coordinated events including cell contractions and cell protrusions...
- Identifying an uptake mechanism for the antiepileptic and bipolar disorder treatment valproic acid using the simple biomedical model DictyosteliumNicole Terbach
Centre for Biomedical Sciences, School of Biological Sciences, Royal Holloway University of London, Egham TW200EX, UK
J Cell Sci 124:2267-76. 2011..of cellular, molecular and genetic approaches to characterize VPA uptake using a simple biomedical model, Dictyostelium discoideum. We show that VPA is taken up against an electrochemical gradient in a dose-dependent manner...
- Myosin II-independent cytokinesis in Dictyostelium: its mechanism and implicationsT Q Uyeda
Biomolecular Research Group, National Institute for Advanced Interdisciplinary Research, Tsukuba, Ibaraki, Japan
Cell Struct Funct 25:1-10. 2000Similar to higher animal cells, ameba cells of the cellular slime mold Dictyostelium discoideum form contractile rings containing filaments of myosin II during mitosis, and it is generally believed that contraction of these rings bisects ..
- Adhesion-dependent and contractile ring-independent equatorial furrowing during cytokinesis in mammalian cellsMasamitsu Kanada
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
Mol Biol Cell 16:3865-72. 2005..Nonetheless, myosin II-null cells of the cellular slime mold Dictyostelium divide efficiently when adhering to substrates by making use of polar traction forces...
- Receptor-dependent and tyrosine phosphatase-mediated inhibition of GSK3 regulates cell fate choiceLeung Kim
Laboratory of Cellular and Developmental Biology, Building 50 3351, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
Dev Cell 3:523-32. 2002Asymmetric body axis formation is central to metazoan development. Dictyostelium establishes an anterior/posterior axis utilizing seven-transmembrane cAMP morphogen receptors (CARs) and GSK3-mediated signal transductions that has a ..
- Dictyostelium myosin-IE is a fast molecular motor involved in phagocytosisUlrike Dürrwang
Abteilung Biophysik, Max Planck Institut fur medizinische Forschung, Jahnstr 29, 69120 Heidelberg, Germany
J Cell Sci 119:550-8. 2006..A complete analysis of the kinetic and motor properties of Dictyostelium discoideum myosin-IE was achieved by the use of motor domain constructs with artificial lever arms...
- The Parkinson disease gene LRRK2: evolutionary and structural insightsIgnacio Marín
Departamento de Genetica, Universidad de Valencia, Burjassot, Spain
Mol Biol Evol 23:2423-33. 2006..Genes in the slime mold Dictyostelium discoideum with structures very similar to those found in animal LRRK genes, including the protein kinase ..
- Dictyostelium discoideum as a model system for identification of Burkholderia pseudomallei virulence factorsBenjamin M Hasselbring
Department of Microbiology, 210 Biological Sciences Building, University of Georgia, Athens, GA 30602, USA
Infect Immun 79:2079-88. 2011..the closely related nearly avirulent species Burkholderia thailandensis to predation by the phagocytic amoeba Dictyostelium discoideum...
- Sensitization of Dictyostelium chemotaxis by phosphoinositide-3-kinase-mediated self-organizing signalling patchesMarten Postma
Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Cell Sci 117:2925-35. 2004The leading edge of Dictyostelium cells in chemoattractant gradients can be visualized using green fluorescent protein (GFP) tagged to the pleckstrin-homology (PH) domain of cytosolic regulator of adenylyl cyclase (CRAC), which ..
- Dictyostelium discoideum--a model for many reasonsSarah J Annesley
Department of Microbiology, La Trobe University, Bundoora, VIC 3086, Australia
Mol Cell Biochem 329:73-91. 2009The social amoeba or cellular slime mould Dictyostelium discoideum is a "professional" phagocyte that has long been recognized for its value as a biomedical model organism, particularly in studying the actomyosin cytoskeleton and ..
- Myosin II is essential for the spatiotemporal organization of traction forces during cell motilityRuedi Meili
Section of Cell and Developmental Biology, Division of Biological Sciences, Department of Mechanical and Aerospace Engineering, and Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
Mol Biol Cell 21:405-17. 2010..However, it does not reduce significantly the magnitude of the traction forces, uncovering a non-MyoII-mediated mechanism for the contractility of the cell...
- Chemotaxis, chemokine receptors and human diseaseTian Jin
Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, 12441 Parklawn Drive, Rockville, MD 20852, USA
Cytokine 44:1-8. 2008..In this review, we wish to mention landmark discoveries in the chemotaxis research field that shaped our current understanding of this fundamental cell behavior and lay out key questions that remain to be addressed in the future...
- The role of polyphosphate and acidocalcisomes in Trypanosoma bruceiRoberto Docampo; Fiscal Year: 2013..several pathogenic microorganisms as well as in the green alga Chlamydomonas reinhardtii, and the slime mold Dictyostelium discoideum...
- The Molecular Basis of Allorecognition in Social AmoebaGad Shaulsky; Fiscal Year: 2013..In the previous grant period we found an allorecognition system in the social soil amoeba Dictyostelium discoideum...
- Role of mucin-type O-glycosylation in Trypanosoma cruzi biologyChristopher M West; Fiscal Year: 2012..cruzi will be analyzed, by testing its predicted 13GalT activity after heterologous expression in Dictyostelium, and investigating its biochemical and cellular function by targeted gene disruption and over-expression in T...
- Molecular Mechanisms of Host Function Exploitation by Type IV effectors of LegionZhao Qing Luo; Fiscal Year: 2013..was defective in intracellular growth in both mouse bone marrow-derived macrophages and the amoebae host Dictyostelium discoideum...
- Cellular and Biochemical Studies of Myosin Assembly in DictyosteliumThomas Egelhoff; Fiscal Year: 2009..We are using the simple amoeba Dictyostelium discoideum as a model system for identifying signaling pathways that regulate myosin assembly...
- Quantitative Analysis of Chemotactic Motility Cycle of Ameboid CellsJUAN CARLOS LASHERAS; Fiscal Year: 2013..b>Dictyostelium discoideum has proven to be an excellent model for the chemotactic migration of amoeboid cells such as ..
- Negative regulation of virulence in Pseudomonas aeruginosaAlbert Siryaporn; Fiscal Year: 2013..in cells that are attached to abiotic surfaces Surface-attached cells are also more virulent towards the host Dictyostelium discoideum. I will test the hypothesis that virulence is regulated by surface attachment...
- CpnA's role in cAMP signaling and the actin cytoskeleton in DictyosteliumCynthia K Damer; Fiscal Year: 2013..The model organism Dictyostelium discoideum has six copine genes (cpnA-cpnF) and provides an ideal system for studying copine function...
- Interaction between Vibrio cholerae and Dictylostelium discoideumSHELLEY M contact PAYNE; Fiscal Year: 2010..Our studies will focus on the relationship between V. cholerae and the model protozoan amoeba Dictyostelium discoideum, and our laboratories have combined expertise in these two organisms. Our hypothesis is that V...
- Lipid Signaling in ChemotaxisMiho Iijima; Fiscal Year: 2013..Using Dictyostelium discoideum as our experimental model system, we have demonstrated that phosphatidylinositol 3,4,5 triphosphate ..
- Temporal and Spatial Signaling in ChemotaxisPeter N Devreotes; Fiscal Year: 2013..How do G-proteins link to the network and what is the inhibitory mechanism that causes adaptation? Studies of Dictyostelium amoebae are designed to address these central questions, with the results assessed for generality in human ..
- Structural and functional effects of oxidative modification of myosinREBECCA J MOEN; Fiscal Year: 2013..I will test this hypothesis by performing site-directed Met mutagenesis on myosin in Dictyostelium cell culture, then performing functional and structural tests to determine the correlation between structural ..
- Glycoregulation of Skp1 in the cytoplasm and nucleusChristopher M West; Fiscal Year: 2012..The initial organism of analysis is the social amoeba Dictyostelium, and the target of the pathway studied here is Skp1, an adaptor of the SCF class of E3 ubiquitin ligases whose ..
- Actions of the Sodium-Hydrogen Exchanger Subtype, NHE1Diane L Barber; Fiscal Year: 2012..We showed a leading-edge H+ efflux by NHE1 in mammalian fibroblasts and in Dictyostelium cells is necessary for three stages in cell migration: polarity, actin filament assembly driving membrane ..
- Cytosolic Proline Hydroxylation and GlycosylationChristopher M West; Fiscal Year: 2013..Considerable evidence indicates that CRL Ub-ligases are also regulated. Our findings in the model organism Dictyostelium reveal new and novel mechanisms for regulating the SCF class itself...
- PI(3,4,5)P3 Regulates Cell Polarity During Chemotaxis and CytokinesisChristopher Janetopoulos; Fiscal Year: 2012..We are using the amoeba Dictyostelium as a model system to understand the basic mechanisms that control these changes in cell shape...
- CATENINS, CADHERINS AND THEIR INTERACTIONSWilliam I Weis; Fiscal Year: 2012..of cell adhesion assemblies will be studied by examining homologs of 1- and 2- catenin in the slime mold Dictyostelium discoideum. 3...
- Determinants for the assembly and maturation of clathrin coated vesicles.THERESA JOYCE O'HALLORAN; Fiscal Year: 2013..Using Dictyostelium cells as a model system, three specific aims are proposed: (1) To define how Hip1r regulates coupling of actin ..
- Spatial regulation of cytoskeleton during chemotaxisChang Y Chung; Fiscal Year: 2012..is necessary for many biological functions, including wound healing, axonal guidance, and the aggregation of Dictyostelium cells. Chemotaxis also plays a role in disease states such as arthritis, cancer, and multiple sclerosis...
- Cellular Regulation of Dictyostelium Myosin Heavy Chain KinasesPaul A Steimle; Fiscal Year: 2012..cellular migration) can be regulated to newly indentified candidate myosin II heavy chain kinases (MHCKs) in Dictyostelium, and by extension, how these processes can go awry in cancer cells exhibiting uncontrolled cell division and ..
- Signaling Networks in Chemotaxis and CytokinesisPeter N Devreotes; Fiscal Year: 2013..Studies oi Dictyostelium discoideum, with its accessible biochemistry, cell biology, and genetics, have contributed enormously to the ..
- The Dictyostelium Stock CenterRex L Chisholm; Fiscal Year: 2013DESCRIPTION (provided by applicant): The simple eukaryote Dictyostelium discoideum is a model organism widely used for the study of cellular processes such as cell motility, chemotaxis, cellular differentiation, signal transduction, the ..
- FUNCTIONAL GENOMICS OF DICTYOSTELIUMBlaz Zupan; Fiscal Year: 2013..Program Project is aimed at understanding the mechanisms that control growth and multicellular development in Dictyostelium. In the previous project period we took a functional genomics approach to high-throughput mutant phenotyping, ..
- Study of Cell Adhesion Through an Analysis of Myosin 7Margaret A Titus; Fiscal Year: 2010..conserved unconventional myosin, myosin VII (M7), in amoeboid adhesion has been discovered in the amoeba Dictyostelium. The goal of this project is to define the role of M7 amoeboid adhesion...
- Development of DictyBase, an online informatics resourceRex L Chisholm; Fiscal Year: 2013..dictyBase is the model organism database that uses the genome sequence of Dictyostelium discoiedum to organize biological knowledge resulting from studies using Dictyostelium and related species...
- Mining a collection of soil amoebae for antibacteria agentsMarcin S Filutowicz; Fiscal Year: 2012..b>Dictyostelium discoideum exhibits the largest repository of polyketide synthase (PKS) enzymes of all known genomes...
- Mechanisms of cancer cell chemotaxis in perineural invasionRichard J Wong; Fiscal Year: 2013..Mechanisms of chemotaxis have been previously defined primarily in the non-malignant systems of dictyostelium discoideum (slime mold) and leukocytes...
- SIGNALLING PROCESSES IN DICTYOSTELIUMRichard A Firtel; Fiscal Year: 2013..Naive, randomly moving cells, such as leukocytes and Dictyostelium cells must be able to directionally sense extremely shallow extracellular chemoattractant gradients and ..
- An open microfluidic device to study chemotaxis of eukaryotic cellsDawit Jowhar; Fiscal Year: 2012..These studies will use the social amoeba Dictyostelium discoideum and will be conducted using a newly developed open microfluidic device made out of ..
- Chemical biology of bacterial symbiontsJon Clardy; Fiscal Year: 2013..several genera - Pseudomonas, Burkholderia, and Flavobacteria among others - are used by some social amoebas (Dictyostelium discoideum) to defend territory, deter predators, and provide a selective advantage over close relatives ..
- Intracellular signaling during phagocytosisKevin Swier; Fiscal Year: 2012..The PI was recently introduced to the model organism Dictyostelium discoideum and has been using this organism to introduce students in classrooms and summer workshops to basic ..
- Textpresso information retrieval and extraction system for biological literatureHans Michael Muller; Fiscal Year: 2012..with the respective model organism databases, we will set up literature search engine for zebrafish, rat and Dictyostelium and consider systems for important diseases such as cancer, Alzheimer's and AIDS...
- An autocrine repressor of cell proliferationRichard H Gomer; Fiscal Year: 2009..The PI has found that growing Dictyostelium cells secrete a protein called AprA for autocrine proliferation repressor...
- Elucidation of a Dictyostelium chaloneRichard H Gomer; Fiscal Year: 2013..In the simple eukaryotic model system Dictyostelium discoideum, cells stop proliferating when they reach a high cell density even if adequate nutrients are presen+..
- The Role of COTL1 in T cell development and activationVirginia Smith Shapiro; Fiscal Year: 2013..Coactosin was initially identified in Dictyostelium, as an inhibitor of actin capping, but its function in T cells was unknown...
- The role of kin-recognition in social evolutionGad Shaulsky; Fiscal Year: 2013..mechanism, which is mediated by a pair of polymorphic cell-cell adhesion proteins in the social amoeba Dictyostelium discoideum. We have also discovered dozens of mutants that cheat through various molecular mechanisms...
- Using Controlled 2D and 3D Nanotopography to Unravel Tactile Senses of Motile CelWolfgang Losert; Fiscal Year: 2013..We propose to use the model organism Dictyostelium discoideum to understand, how nanotopographic cues affect cell migration at many levels, from the molecular ..
- MYOSIN ISOZYMESHUGH LEE SWEENEY; Fiscal Year: 2010..We will make use of the fact that myosin V, non-muscle myosin MB, smooth muscle myosin II and Dictyostelium myosin II have kinetics that are fundamentally different from each other...
- A Toolkit for Light-Control of Molecular Processes in Living CellsORION DAVID WEINER; Fiscal Year: 2013..We have demonstrated that this system works in yeast, Dictyostelium, and mammalian cells...
- Excitability in Dictyostelium DevelopmentDAVID JASON SCHWAB; Fiscal Year: 2013..development and how can we manipulate, disrupt, or correct them? I propose to study the collective behavior of Dictyostelium discoideum - a classic model organism for cell-cell signaling - focusing on how single-cell dynamics influence ..
- PP2A regulates development and stress responsesLeung Kim; Fiscal Year: 2012..In contrast, Dictyostelium discoideum, a facultative multicellular organism, has a single B56 homologue and cells lacking B56 were ..
- Controlling collective behavior in eukaryotic cell populationsThomas Gregor; Fiscal Year: 2013..dynamics of single cells give rise to coherent behaviors at the multi-cell stage in the social amoeba Dictyostelium discoideum...
- Pathogenesis to cooperation in the microbiome of a soil amoebaSUSANNE DISALVO; Fiscal Year: 2013..Interestingly, carrier clones of the social amoeba Dictyostelium discoideum have been shown to harbor a variety of bacteria, in contrast to their non-carrier counterparts...
- INTRACELLULAR ROLE OF CLATHRIN AND ITS REGULATIONTHERESA O HALLORAN; Fiscal Year: 2007..Our experimental strategy is to use Dictyostelium as a model system...
- Chemotaxis in Dictyostelium discoideum: an integrative approachWouter Jan Rappel; Fiscal Year: 2013..main aim of this Program Project is to extend our quantitative studies of chemotaxis using the model system Dictyostelium discoideum...
- METABOLIC DETERMINANTS OF HEMATOPOIETIC CELL DEVELOPMENTDaniel Wright; Fiscal Year: 1999..In lower eukaryotes, e.g. S. cerevisiae and Dictyostelium, developmental switches are triggered by metabolic changes caused by nutrient deprivation, and studies of ..
- BIOCHEMICAL BASIS OF DEVELOPMENT IN DICTYOSTELIUMWilliam Loomis; Fiscal Year: 1992Techniques for the reproducible transformation of Dictyostelium cells with cloned sequences have allowed us to determine essential cis-acting sequences in the 5' flanking region of a developmentally controlled gene (actin 15) that are ..
- SEQUENCING THE DICTYOSTELIUM GENOMEAdam Kuspa; Fiscal Year: 2004..b>Dictyostelium is a social amoeba whose study has contributed to our understanding of diverse biological processes common to ..
- Molecular Basis for Protein-Phospholipid InteractionJon Lomasney; Fiscal Year: 2005..C (PLC), and the small G protein ARF; as a regulator of the actin cytoskeleton directing cytokinesis in Dictyostelium, and acting as a regulator of endo and exocytosis...
- Characterization of single-cell dynamics that lead to collective behaviorsAllyson E Sgro; Fiscal Year: 2013..contribute to the coordination of collective behaviors in multicellular systems such as the social amoeba Dictyostelium discoideum...
- Microbial Pattern Recognition by the Social Amoeba Dictyostelium discoideum - ModMICHELLE LYNN DYKSTRA SNYDER; Fiscal Year: 2010..Our proposal here to use the social amoeba Dictyostelium discoideum as a model system to study the molecular mechanisms underlying microbial pattern recognition in ..
- MOLECULAR ANALYSIS OF CYTOKINESIS IN DICTYOSTELIUMArturo De Lozanne; Fiscal Year: 2006..Toward that goal, three proteins required for cytokinesis in Dictyostelium have been identified...
- CYTOKINESIS IN DICTYOSTELIUM--A MOLECULAR ANALYSISJames Spudich; Fiscal Year: 2001..Key to a long term undertaking to solve this problem is the judicious choice of a model system. Dictyostelium discoideum is a model of choice because it's behavioral characteristics during mitosis and cytokinesis are ..
- DICTYOSTELIUM GA2:CELLULAR LOCALIZATION & PALMITOYLATIONROBERT GUNDERSEN; Fiscal Year: 2000DESCRIPTION (from applicant's abstract): The protozoan, Dictyostelium discoideum, provides a powerful system in which to examine fundamental signal transduction processes of the eukaryotic cell...
- CHEMISTRY AND FUNCTION OF DICTYOSTELIUM CALMODULINMargaret Clarke; Fiscal Year: 1992..This project would characterize calmodulin from the eukaryotic microorganism Dictyostelium discoideum and explore its roles, particularly in the regulation of motility...
- COMPUTER ANALYSIS OF AGING IN DICTYOSTELIUMBarbara Wright; Fiscal Year: 1992..events during the metabolic transitions essential to differentiation, aging and death in the model system, Dictyostelium discoideum...
- Role of cGMP signaling in Dictyostelium chemotaxisJanet Smith; Fiscal Year: 2007..such as wound healing, embryogenesis, axonal guidance, metastasis of cancer cells, and aggregation of Dictyostelium cells to form a multicellular organism...
- REGULATION OF SIGNAL TRANSDUCTION IN DICTYOSTELIUMCHARLES SAXE; Fiscal Year: 2000..A large number of G protein-linked receptors have been characterized, from organisms as diverse as yeast, Dictyostelium, Drosophila and humans, and include multi gene families for opsins, and the adrenergic, muscarinic ..
- RACE PATHWAY IN THE REGULATION OF CYTOKINESISDenis Larochelle; Fiscal Year: 1999..Recently, the eukaryotic organism Dictyostelium discoideum has emerged as one of the premier model systems for studying cytokinesis...
- An optogenetic method to rapidly and reversibly titrate protein levels in cellsORION DAVID WEINER; Fiscal Year: 2013..We propose to establish and optimize this approach in 3 model systems - Dictyostelium, budding yeast, and mammalian cells to test the modularity of this system, demonstrate its versatility, and ..
- DEVELOPMENTAL GENE PROGRAMWilliam Loomis; Fiscal Year: 1999..Recent advances in molecular genetic techniques in Dictyostelium make this a favored organism for detailed studies on genes involved in cellular differentiation and ..
- Mechanisms of Self/Nonself Recognition in Dictyostelium discoideumROCIO BENABENTOS; Fiscal Year: 2009..The social amoeba Dictyostelium possess a self/nonself recognition mechanism based on cell adhesion and signaling that promotes the ..
- MECHANISMS OF ETHANOL TOXICITY IN DICTYOSTELIUMGad Shaulsky; Fiscal Year: 2001..is to identify molecular targets for ethanol toxicity by means of genetic screens in the model organism Dictyostelium discoideum...
- EUKARYOTIC TISSUE DENSITY SENSORRichard Gomer; Fiscal Year: 1992..As a model system, we will use the conditioned medium factor (CMF) secreted by developing Dictyostelium discoideum cells...
- Analysis of signaling during chemotaxis in DictyosteliumSTACEY WILLARD; Fiscal Year: 2007..Studies in Dictyostelium have improved our understanding of the signaling network that governs chemotaxis...
- REGULATION OF DICTYOSTELIUM MYOSIN BY PHOSPHORYLATIONJames Spudich; Fiscal Year: 2000..The specific aim of this proposal is to study the roles of myosin phosphorylation in Dictyostelium, an organism that allows the convergence of biochemical, molecular genetic, and physiological approaches...