Genomes and Genes
trypanosoma brucei brucei
Summary: A hemoflagellate subspecies of parasitic protozoa that causes nagana in domestic and game animals in Africa. It apparently does not infect humans. It is transmitted by bites of tsetse flies (Glossina).
Publications282 found, 100 shown here
- The transcriptome of the human pathogen Trypanosoma brucei at single-nucleotide resolutionNikolay G Kolev
School of Public Health, Yale University, New Haven, Connecticut, United States of America
PLoS Pathog 6:e1001090. 2010..and revealed heterogeneity in pre-mRNA processing that could potentially contribute to the survival and success of the parasite population in the insect vector and the mammalian host...
- High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosomeSam Alsford
London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
Genome Res 21:915-24. 2011..The current data set also provides much needed genetic validation to identify new drug targets. RIT-seq represents a versatile new tool for genome-scale functional analyses and for the exploitation of genome sequence data...
- Four histone variants mark the boundaries of polycistronic transcription units in Trypanosoma bruceiT Nicolai Siegel
Laboratory of Molecular Parasitology, The Rockefeller University, New York, New York 10065, USA
Genes Dev 23:1063-76. 2009....
- Spliced leader trapping reveals widespread alternative splicing patterns in the highly dynamic transcriptome of Trypanosoma bruceiDaniel Nilsson
Institute of Cell Biology, University of Bern, Bern, Switzerland
PLoS Pathog 6:e1001037. 2010..The entire dataset can be accessed online at TriTrypDB or through: http://splicer.unibe.ch/...
- Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sitesTim Nicolai Siegel
Laboratory of Molecular Parasitology, The Rockefeller University, New York, NY 10065, USA
Nucleic Acids Res 38:4946-57. 2010..Our study demonstrates the usefulness of the RNA-seq technology to study the transcriptional landscape of an organism whose genome has not been fully annotated...
- Telomeric expression sites are highly conserved in Trypanosoma bruceiChristiane Hertz-Fowler
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, United Kingdom
PLoS ONE 3:e3527. 2008..This work opens important perspectives in understanding the molecular mechanisms of antigenic variation, a widely used strategy for immune evasion in pathogens, and telomere biology...
- The genome of the African trypanosome Trypanosoma bruceiMatthew Berriman
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
Science 309:416-22. 2005..brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified...
- Heat shock causes a decrease in polysomes and the appearance of stress granules in trypanosomes independently of eIF2(alpha) phosphorylation at Thr169Susanne Kramer
Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
J Cell Sci 121:3002-14. 2008....
- Trypanocidal drugs: mechanisms, resistance and new targetsShane R Wilkinson
Queen Mary Pre Clinical Drug Discovery Group, School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, UK
Expert Rev Mol Med 11:e31. 2009....
- Widespread variation in transcript abundance within and across developmental stages of Trypanosoma bruceiBryan C Jensen
Seattle Biomedical Research Institute, 307 Westlake Ave North, Seattle, WA 98109, USA
BMC Genomics 10:482. 2009..Trypanosomatids represent a unique case in eukaryotes in that they transcribe protein-coding genes as large polycistronic units, and rarely regulate gene expression at the level of transcription initiation...
- The role of the 5'-3' exoribonuclease XRNA in transcriptome-wide mRNA degradationTheresa Manful
Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Alliance, D69120 Heidelberg, Germany
RNA 17:2039-47. 2011....
- Alba-domain proteins of Trypanosoma brucei are cytoplasmic RNA-binding proteins that interact with the translation machineryJan Mani
Institute of Cell Biology, University of Bern, Bern, Switzerland
PLoS ONE 6:e22463. 2011..Our results are also consistent with stage-specific regulation of translation in trypanosomes, but most likely in the context of initiation...
- Antigenic diversity is generated by distinct evolutionary mechanisms in African trypanosome speciesAndrew P Jackson
Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom
Proc Natl Acad Sci U S A 109:3416-21. 2012....
- Comparative SILAC proteomic analysis of Trypanosoma brucei bloodstream and procyclic lifecycle stagesMichael D Urbaniak
Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee, United Kingdom
PLoS ONE 7:e36619. 2012..Our proteomic data is broadly in agreement with transcriptomic studies, but with significantly larger fold changes observed at the protein level than at the mRNA level...
- The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome researchM A Ferguson
Division of Molecular Parasitology and Biological Chemistry, Department of Biochemistry, The Wellcome Trust Building, University of Dundee, Dundee DD1 5EH, UK
J Cell Sci 112:2799-809. 1999..Apart from providing stable membrane anchorage, GPI anchors have been implicated in the sequestration of GPI-anchored proteins into specialised membrane microdomains, known as lipid rafts, and in signal transduction events...
- Functional genomics in Trypanosoma brucei: a collection of vectors for the expression of tagged proteins from endogenous and ectopic gene lociSteven Kelly
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
Mol Biochem Parasitol 154:103-9. 2007
- RAP1 is essential for silencing telomeric variant surface glycoprotein genes in Trypanosoma bruceiXiaofeng Yang
Department of Biological, Geological, and Environmental Sciences, Center for Gene Regulation in Health and Diseases, Cleveland State University, Cleveland, OH 44115, USA
Cell 137:99-109. 2009..This graduated silencing pattern suggests that telomere integrity plays a key role in tbRAP1-dependent silencing and VSG regulation...
- Sleeping sicknessD Malvy
Travel Clinics and Division of Tropical Medicine and Imported Diseases, Department of Internal Medicine and Tropical Diseases, University Hospital Centre, Bordeaux, France
Clin Microbiol Infect 17:986-95. 2011..New methods to diagnose and treat patients and to control transmission by the tsetse fly are needed to achieve the goal of global elimination of the disease...
- Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse flyLori Peacock
School of Biological Sciences, University of Bristol, Bristol BS8 1UG, United Kingdom
Proc Natl Acad Sci U S A 108:3671-6. 2011..This is evidence of conventional meiotic division in an excavate protist, and the functional conservation of the meiotic machinery in these divergent organisms underlines the ubiquity and basal evolution of meiosis in eukaryotes...
- The cell biology of Trypanosoma brucei differentiationKatelyn Fenn
Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Ashworth Laboratories, King s Buildings, West Mains Road, Edinburgh EH9 3JT, UK
Curr Opin Microbiol 10:539-46. 2007..Interestingly, the new molecular discoveries provide insights into the biology of the parasite in the field...
- Ubiquitylation and developmental regulation of invariant surface protein expression in trypanosomesKa Fai Leung
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, United Kingdom
Eukaryot Cell 10:916-31. 2011..We suggest that ubiquitylation may be a general mechanism for regulating trans-membrane domain surface proteins in trypanosomes...
- The small chromosomes of Trypanosoma brucei involved in antigenic variation are constructed around repetitive palindromesBill Wickstead
Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, United Kingdom
Genome Res 14:1014-24. 2004..Moreover, palindromy appears to be a feature of (peri)centromeres in other species that can be easily overlooked. We propose that sequence inversion is one of the higher-order sequence motifs that confer chromosomal stability...
- Flagellar motility is required for the viability of the bloodstream trypanosomeRichard Broadhead
Department of Biological Sciences, Lancaster University, Lancaster LA1 4YQ, UK
Nature 440:224-7. 2006..A postgenomic meta-analysis, comparing the evolutionarily ancient trypanosome with other eukaryotes including humans, identifies numerous trypanosome-specific flagellar proteins, suggesting new avenues for selective intervention...
- Gene expression in Trypanosoma brucei: lessons from high-throughput RNA sequencingT Nicolai Siegel
Biology of Host Parasite Interactions, Parasitology, Institut Pasteur, 75724 Paris, France
Trends Parasitol 27:434-41. 2011..brucei...
- NLP is a novel transcription regulator involved in VSG expression site control in Trypanosoma bruceiMani Shankar Narayanan
Division of Cell and Molecular Biology, Sir Alexander Fleming Building, Imperial College London, South Kensington, London SW7 2AZ, UK
Nucleic Acids Res 39:2018-31. 2011..Discovery of the unusual transcription regulator NLP provides new insight into the factors that are critical for ES control...
- The FACT subunit TbSpt16 is involved in cell cycle specific control of VSG expression sites in Trypanosoma bruceiViola Denninger
Division of Cell and Molecular Biology, Sir Alexander Fleming Building, Imperial College, South Kensington, London SW72AZ, UK
Mol Microbiol 78:459-74. 2010..The chromatin remodeler FACT is therefore implicated in maintenance of repressed chromatin present at silent VSG ES promoters, but is also essential for chromosome segregation presumably through maintenance of functional centromeres...
- Conserved and specific functions of axoneme components in trypanosome motilityCarole Branche
INSERM U565 and CNRS UMR5153 and MNHN USM0503, Museum national d histoire naturelle, 43 rue Cuvier, 75231 Paris Cedex 05, France
J Cell Sci 119:3443-55. 2006..Finally, our study also reveals the conservation of molecular components of the trypanosome flagellum. Coupled to the ease of reverse genetics, it raises the interest of trypanosomes as model organisms to study cilia and flagella...
- Chemical validation of trypanothione synthetase: a potential drug target for human trypanosomiasisLeah S Torrie
Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
J Biol Chem 284:36137-45. 2009..Taken together, these data provide initial chemical validation of TryS as a drug target in T. brucei...
- Investigation of trypanothione reductase as a drug target in Trypanosoma bruceiDaniel Spinks
Drug Discovery Unit, College of Life Sciences, University of Dundee, Sir James Black Centre, Dundee, DD1 5EH, UK
ChemMedChem 4:2060-9. 2009..Both of these series, containing either a quinoline or pyrimidinopyrazine scaffold, yielded low micromolar inhibitors of the enzyme and growth of the parasite. The challenges of inhibiting TryR with druglike molecules is discussed...
- A yeast-endonuclease-generated DNA break induces antigenic switching in Trypanosoma bruceiCatharine E Boothroyd
Laboratory of Lymphocyte Biology, The Rockefeller University, New York, New York 10065, USA
Nature 459:278-81. 2009....
- A doubly inducible system for RNA interference and rapid RNAi plasmid construction in Trypanosoma bruceiVincent P Alibu
Zentrum fur Molekulare Biologie der Universitat Heidelberg, ZMBH, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
Mol Biochem Parasitol 139:75-82. 2005....
- Three-dimensional cellular architecture of the flagellar pocket and associated cytoskeleton in trypanosomes revealed by electron microscope tomographySylvain Lacomble
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
J Cell Sci 122:1081-90. 2009..Overall, our studies reveal a highly organised, yet dynamic, area of cytoplasm and will be informative in understanding its function...
- The phosphoproteome of bloodstream form Trypanosoma brucei, causative agent of African sleeping sicknessIsabelle R E Nett
Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee, Scotland, United Kingdom
Mol Cell Proteomics 8:1527-38. 2009....
- The trypanosome flagellar pocketMark C Field
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK
Nat Rev Microbiol 7:775-86. 2009..In this Review we discuss the contribution of the flagellar pocket to protein trafficking, immune evasion and other processes...
- N-myristoyltransferase inhibitors as new leads to treat sleeping sicknessJulie A Frearson
Drug Discovery Unit, Division of Biological Chemistry and Drug Discovery, University of Dundee, Dundee DD1 5EH, UK
Nature 464:728-32. 2010..Our studies validate T. brucei N-myristoyltransferase as a promising therapeutic target for human African trypanosomiasis...
- High-throughput decoding of antitrypanosomal drug efficacy and resistanceSam Alsford
London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
Nature 482:232-6. 2012....
- A novel phosphatase cascade regulates differentiation in Trypanosoma brucei via a glycosomal signaling pathwayBalÃ¡zs SzÃ¶or
Centre for Immunity, Infection, and Evolution, Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom
Genes Dev 24:1306-16. 2010..This is the first characterized environmental signaling pathway targeted directly to a peroxisome-like organelle in any eukaryotic cell...
- Assembly of the flagellum and its role in cell morphogenesis in Trypanosoma bruceiSue Vaughan
School of Life Sciences, Oxford Brookes University, Gipsy Lane, Oxford OX3 0BP, UK
Curr Opin Microbiol 13:453-8. 2010..In this review we discuss evidence published in the past four years that is unravelling the role of the flagellum in organelle segregation, inheritance of cell shape and cytokinesis...
- Nuclear DBF-2-related kinases are essential regulators of cytokinesis in bloodstream stage Trypanosoma bruceiJiangtao Ma
Division of Infection and Immunity, Faculty of Biomedical and Life Sciences and Wellcome Trust Centre for Molecular Parasitology, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
J Biol Chem 285:15356-68. 2010....
- Identification of a κ-opioid agonist as a potent and selective lead for drug development against human African trypanosomiasisDeuan C Jones
Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
Biochem Pharmacol 80:1478-86. 2010..Among the other potent, but less selective screening hits were compound classes with activity against protein kinases, topoisomerases, tubulin, as well as DNA and energy metabolism...
- A molecular mechanism for eflornithine resistance in African trypanosomesIsabel M Vincent
University of Glasgow, Glasgow, UK
PLoS Pathog 6:e1001204. 2010..The loss of this transporter will be easily identified in the field using a simple PCR test, enabling more appropriate chemotherapy to be administered...
- Metabolic functions of glycosomes in trypanosomatidsPaul A M Michels
Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology and Laboratory of Biochemistry, Universite Catholique de Louvain, ICP TROP 74 39, Avenue Hippocrate 74, B 1200 Brussels, Belgium
Biochim Biophys Acta 1763:1463-77. 2006..The compartmentation of environment-sensitive parts of the metabolic network within glycosomes would, through this way of organelle renewal, enable the parasites to adapt rapidly and efficiently to the new conditions...
- Elongator protein 3b negatively regulates ribosomal DNA transcription in african trypanosomesSam Alsford
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
Mol Cell Biol 31:1822-32. 2011..ELP3b is also the first trypanosome protein shown to distinguish between rDNA and variant surface glycoprotein transcription within different RNAP-I compartments...
- Characterization of Trypanosoma brucei dihydroorotate dehydrogenase as a possible drug target; structural, kinetic and RNAi studiesTracy L Arakaki
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
Mol Microbiol 68:37-50. 2008..brucei enzyme, which is reported here at a resolution of 1.95 A. Additional research, guided by the crystal structure described herein, is needed to identify potent inhibitors of T. brucei DHODH...
- Rab28 function in trypanosomes: interactions with retromer and ESCRT pathwaysJennifer H Lumb
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
J Cell Sci 124:3771-83. 2011..We suggest that Rab28 coordinates the activity of retromer-dependent trafficking and ESCRT-mediated degradative pathways...
- Genetic control of resistance to Trypanosoma brucei brucei infection in miceMatyáš Síma
Laboratory of Molecular and Cellular Immunology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
PLoS Negl Trop Dis 5:e1173. 2011b>Trypanosoma brucei brucei infects livestock, with severe effects in horses and dogs. Mouse strains differ greatly in susceptibility to this parasite. However, no genes controlling these differences were mapped.
- TbISWI regulates multiple polymerase I (Pol I)-transcribed loci and is present at Pol II transcription boundaries in Trypanosoma bruceiTara M Stanne
Division of Cell and Molecular Biology, Sir Alexander Fleming Building, Imperial College London South Kensington, Imperial College Road, London, United Kingdom
Eukaryot Cell 10:964-76. 2011..Our results indicate that TbISWI is a versatile chromatin remodeler that regulates transcription at multiple Pol I loci and is particularly abundant at many Pol II transcription boundaries in T. brucei...
- Identification of Trypanosoma brucei RMI1/BLAP75 homologue and its roles in antigenic variationHee Sook Kim
Laboratory of Molecular Parasitology, The Rockefeller University, New York, New York, United States of America
PLoS ONE 6:e25313. 2011....
- Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma bruceiMichael D Urbaniak
Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
Mol Biochem Parasitol 166:183-5. 2009..These data show that TbCK1.2 is an attractive target for anti-trypanosomal drug discovery...
- A protein-protein interaction map of trypanosome ~20S editosomesAchim Schnaufer
Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
J Biol Chem 285:5282-95. 2010..These results reveal a physical organization that underlies the coordination of the various catalytic and substrate binding activities within the ~20S editosomes during the editing process...
- Compartmentation prevents a lethal turbo-explosion of glycolysis in trypanosomesJurgen R Haanstra
Department of Molecular Cell Physiology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1085, NL 1081 HV, Amsterdam, The Netherlands
Proc Natl Acad Sci U S A 105:17718-23. 2008..This provides the first experimental support for our hypothesis that pathway compartmentation is an alternative to allosteric regulation...
- Genome-wide in silico screen for CCCH-type zinc finger proteins of Trypanosoma brucei, Trypanosoma cruzi and Leishmania majorSusanne Kramer
Department of Biochemistry, University of Cambridge, Cambridge, UK
BMC Genomics 11:283. 2010..In kinetoplastids, CCCH type zinc finger proteins have been identified as being involved in the regulation of the life cycle and possibly the cell cycle. To date, no systematic listing of CCCH proteins in kinetoplastids is available...
- A vector series for rapid PCR-mediated C-terminal in situ tagging of Trypanosoma brucei genesMichael Oberholzer
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
Mol Biochem Parasitol 145:117-20. 2006
- Deletion of the Trypanosoma brucei superoxide dismutase gene sodb1 increases sensitivity to nifurtimox and benznidazoleS Radhika Prathalingham
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
Antimicrob Agents Chemother 51:755-8. 2007..Here, we identify a superoxide dismutase required to protect Trypanosoma brucei from drug-generated superoxide...
- TOPO3alpha influences antigenic variation by monitoring expression-site-associated VSG switching in Trypanosoma bruceiHee Sook Kim
Laboratory of Molecular Parasitology, The Rockefeller University, New York, New York, United States of America
PLoS Pathog 6:e1000992. 2010..Our data suggest that TOPO3alpha may remove undesirable recombination intermediates constantly arising between active and silent ESs, thereby balancing ES integrity against VSG recombination...
- Comparative genomics of trypanosomatid parasitic protozoaNajib M El-Sayed
Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Science 309:404-9. 2005..Contrary to recent reports, our analyses reveal no evidence that these species are descended from an ancestor that contained a photosynthetic endosymbiont...
- Asymmetric cell division as a route to reduction in cell length and change in cell morphology in trypanosomesReuben Sharma
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
Protist 159:137-51. 2008..Thus, the asymmetric cell division cycle provides a mechanism for a change in cell morphology and also an explanation for how a reduction in cell length can occur in a cell shaped by a stable microtubule array...
- RNA interference identifies two hydroperoxide metabolizing enzymes that are essential to the bloodstream form of the african trypanosomeShane R Wilkinson
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
J Biol Chem 278:31640-6. 2003..These experiments therefore identify redox pathways that are essential for oxidative defense in T. brucei and validate the corresponding peroxidases as targets for drug design...
- Human infectivity trait in Trypanosoma brucei: stability, heritability and relationship to sra expressionC M R Turner
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK
Parasitology 129:445-54. 2004....
- Histone deacetylases play distinct roles in telomeric VSG expression site silencing in African trypanosomesQiao Ping Wang
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
Mol Microbiol 77:1237-45. 2010..We conclude that DAC1 and DAC3 play distinct roles in subtelomeric gene silencing and that DAC3 represents the first readily druggable target linked to VSG ES control in the African trypanosome...
- Expression and role of CXCL10 during the encephalitic stage of experimental and clinical African trypanosomiasisDaniel N Amin
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
J Infect Dis 200:1556-65. 2009..Human African trypanosomiasis, caused by Trypanosoma brucei, involves an early hemolymphatic stage followed by a late encephalitic stage...
- Migration of African trypanosomes across the blood-brain barrierWillias Masocha
Karolinska Institutet, Department of Neuroscience, S 171 77 Stockholm, Sweden
Physiol Behav 92:110-4. 2007....
- Single-locus targeting constructs for reliable regulated RNAi and transgene expression in Trypanosoma bruceiSam Alsford
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
Mol Biochem Parasitol 161:76-9. 2008..The constructs are integrated at a single, marked locus and deliver reliable and reproducible expression...
- Glycolysis modulates trypanosome glycoprotein expression as revealed by an RNAi libraryJames C Morris
Department of Biological Chemistry, Johns Hopkins Medical School, 725 N Wolfe Street, Baltimore, MD 21205, USA
EMBO J 21:4429-38. 2002..These data suggest that T.brucei 'senses' changes in glucose level and modulates procyclin expression accordingly...
- The suppression of galactose metabolism in procylic form Trypanosoma brucei causes cessation of cell growth and alters procyclin glycoprotein structure and copy numberJanine R Roper
Division of Biological Chemistry and Molecular Microbiology, The School of Life Sciences, University of Dundee, Scotland, UK
J Biol Chem 280:19728-36. 2005..The parasites compensated, ultimately unsuccessfully, by expressing 10-fold more procyclin. The implications of these data with respect to the relative roles of procyclin polypeptide and carbohydrate are discussed...
- Differential trypanosome surface coat regulation by a CCCH protein that co-associates with procyclin mRNA cis-elementsPegine Walrad
Centre for Immunology, Infection and Evolution, Institute of Immunology and Infection Research, School of Biological Sciences, Ashworth Laboratories, University of Edinburgh, Edinburgh, UK
PLoS Pathog 5:e1000317. 2009..Combined with the known association of TbZFP3 with the translational apparatus, this study provides a long-sought missing link between surface protein cis-regulatory signals and the gene expression machinery in trypanosomes...
- Molecular diagnostics for sleeping sickness: what is the benefit for the patient?Stijn Deborggraeve
Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium
Lancet Infect Dis 10:433-9. 2010..The current formats are not applicable to field conditions, and simplification, standardisation, and proper test evaluation in the target setting should be the main focus for future development...
- Intraspecific competition between co-infecting parasite strains enhances host survival in African trypanosomesOliver Balmer
Department of Ecology and Evolutionary Biology, Yale University, 165 Prospect Street, New Haven, Connecticut 06511, USA
Ecology 90:3367-78. 2009..Our results are therefore not only of basic ecological interest investigating an important form of intraspecific competition, but may also have applied relevance for public health...
- Ubiquitylation is required for degradation of transmembrane surface proteins in trypanosomesWei Lian Chung
Department of Pathology, University of Cambridge, Cambridge, UK
Traffic 9:1681-97. 2008..Hence, ubiquitylation is an evolutionarily conserved mechanism for internalization of surface proteins, but aspects of the machinery differ substantially between the major eukaryotic lineages...
- Trypanosoma brucei glycogen synthase kinase-3, a target for anti-trypanosomal drug development: a public-private partnership to identify novel leadsRichard O Oduor
Opportunities for Partnering in Medicine, Pfizer Global Research and Development, Sandwich, Kent, United Kingdom
PLoS Negl Trop Dis 5:e1017. 2011..A collaborative industrial/academic partnership facilitated by the World Health Organisation Tropical Diseases Research division (WHO TDR) was initiated to stimulate research aimed at identifying new drugs for treating HAT...
- The distribution of nifurtimox across the healthy and trypanosome-infected murine blood-brain and blood-cerebrospinal fluid barriersSinthujah Jeganathan
Pharmaceutical Sciences Research Division, King s College London, London, United Kingdom
J Pharmacol Exp Ther 336:506-15. 2011..Consequently, CNS efficacy may be improved with nifurtimox-pentamidine combinations, but over time may be reduced when nifurtimox is combined with eflornithine, suramin, or melarsoprol...
- High affinity nanobodies against the Trypanosome brucei VSG are potent trypanolytic agents that block endocytosisBenoit Stijlemans
Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussels, Brussels, Belgium
PLoS Pathog 7:e1002072. 2011....
- Independent analysis of the flagellum surface and matrix proteomes provides insight into flagellum signaling in mammalian-infectious Trypanosoma bruceiMichael Oberholzer
Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, CA 90095, USA
Mol Cell Proteomics 10:M111.010538. 2011..The combined results indicate that the flagellum surface presents a diverse and dynamic host-parasite interface that is well-suited for host-parasite signaling...
- Development of novel drugs for human African trypanosomiasisReto Brun
Department Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, and, University of Basel, CH 4002 Basel, Switzerland
Future Microbiol 6:677-91. 2011..With other screening programs yielding hits, the pipeline for new HAT drugs might finally begin to fill...
- Characterization of tGLP-1, a Golgi and lysosome-associated, transmembrane glycoprotein of African trypanosomesA Lingnau
Washington University School of Medicine, Department of Molecular Microbiology, St Louis, MO 63110, USA
J Cell Sci 112:3061-70. 1999Purification of endosomal/lysosomal vesicles of Trypanosoma brucei brucei bloodstream forms and generation of monoclonal antibodies led to the isolation of antibodies directed against an 85 kDa, Golgi and endocytic traffic-associated ..
- Endonuclease associations with three distinct editosomes in Trypanosoma bruceiJason Carnes
Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
J Biol Chem 286:19320-30. 2011..These results and published data suggest that KREPB6, KREPB7, and KREPB8 associate with the deletion subcomplex, whereas the KREN1, KREN2, and KREN3 endonucleases associate with the insertion subcomplex...
- Chaperone requirements for biosynthesis of the trypanosome variant surface glycoproteinMark C Field
Department of Pathology, University of Cambridge, Cambridge, United Kingdom
PLoS ONE 5:e8468. 2010..As the variant surface glycoprotein (VSG) represents approximately 90% of trypanosome plasma membrane protein, it is possible that VSG has evolved to fold efficiently to minimize ER folding burden...
- Transposon mutagenesis of Trypanosoma brucei identifies glycosylation mutants resistant to concanavalin ASimone Leal
Laboratory of Molecular Parasitology, The Rockefeller University, New York, New York 10021, USA
J Biol Chem 279:28979-88. 2004..25%. As we approach the completion of the trypanosome genome project, transposon mutagenesis provides an important addition to the repertoire of genetic tools for T. brucei...
- Transmission stages dominate trypanosome within-host dynamics during chronic infectionsPaula MacGregor
Centre for Immunity, Infection, and Evolution, Institute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK
Cell Host Microbe 9:310-8. 2011..Our data reveal the dominance of transmission stages throughout infection, a consequence being austere use of the parasite's antigen repertoire...
- Nuclear architecture, genome and chromatin organisation in Trypanosoma bruceiKlaus Ersfeld
Department of Biological Sciences and Hull York Medical School, University of Hull, Hull HU6 7RX, UK
Res Microbiol 162:626-36. 2011..These advances also have a major impact on progress in understanding the molecular basis of antigenic variation...
- Mitochondrial preprotein translocase of trypanosomatids has a bacterial originMascha Pusnik
Department of Chemistry and Biochemistry, University of Bern, CH 3012 Bern, Switzerland
Curr Biol 21:1738-43. 2011..This suggests that the protein import channel in trypanosomes is a relic of an archaic protein transport system that was operational in the ancestor of all eukaryotes...
- A zinc finger protein, TbZC3H20, stabilizes two developmentally regulated mRNAs in trypanosomesAlexandra S Ling
Division of Cell and Molecular Biology, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
J Biol Chem 286:20152-62. 2011..This suggests a broader role for ZC3Hs in post-transcriptional regulation of gene expression than previously thought...
- Dissecting the essentiality of the bifunctional trypanothione synthetase-amidase in Trypanosoma brucei using chemical and genetic methodsSusan Wyllie
Division of Biological Chemistry and Drug Discovery, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, Scotland, UK
Mol Microbiol 74:529-40. 2009..The synthetase function of TRYS is thus unequivocally validated as a drug target by both chemical and genetic methods...
- Cohesin regulates VSG monoallelic expression in trypanosomesDavid Landeira
Instituto de Parasitologia y Biomedicina Lopez Neyra, Consejo Superior de Investigaciones Cientificas, 18100 Granada, Spain
J Cell Biol 186:243-54. 2009..Thus, in addition to maintaining sister chromatid cohesion during mitosis, the cohesin complex plays an essential role in the correct epigenetic inheritance of the active transcriptional VSG ES state...
- Comparative genomics reveals two novel RNAi factors in Trypanosoma brucei and provides insight into the core machineryRebecca L Barnes
Department of Internal Medicine, Yale University, New Haven, Connecticut, USA
PLoS Pathog 8:e1002678. 2012..The availability of the core RNAi machinery in T. brucei provides a platform to gain mechanistic insights in this ancient eukaryote and to identify the minimal set of components required to reconstitute RNAi in RNAi-deficient parasites...
- Structural analysis of the asparagine-linked glycans from the procyclic Trypanosoma brucei and its glycosylation mutants resistant to Concanavalin A killingK Y Hwa
Institute of Biological Chemistry, Academia Sinica, 128 Yen Chiu Yuan Road, Section 2, Nankang, Taipei, 115, Taiwan, ROC
Mol Biochem Parasitol 111:173-84. 2000....
- Partial biochemical characterization of a metalloproteinase from the bloodstream forms of Trypanosoma brucei brucei parasitesKarina Pires de Sousa
Unidade de Ensino e Investigação de Clínica das Doenças Tropicais, Centro de Malaria e Outras Doencas Tropicais, Instituto de Higiene e Medicina Tropical, Rua da Junqueira, Lisbon, Portugal
Protein J 29:283-9. 2010..the proteolytic activity of the crude trypanosome protein extract obtained from the bloodstream forms of Trypanosoma brucei brucei parasites...
- The Trypanosoma brucei autoantigen I/6 is an internally repetitive cytoskeletal proteinE Detmer
Institut fur Allgemeine Mikrobiologie, Universitat Bern, Switzerland
Eur J Cell Biol 72:378-84. 1997..Protein I/6 contains a non-functional EF-hand calcium binding domain and a domain of six tandemly arranged repeat units of eight amino acids length...
- Exportin 1 mediates nuclear export of the kinetoplastid spliced leader RNAGusti M Zeiner
Department of Microbiology, University of California at Los Angeles, Los Angeles, California 90095 1489, USA
Eukaryot Cell 2:222-30. 2003..This study demonstrates spatial and mechanistic similarities between the posttranscriptional trafficking of the kinetoplastid protozoan SL RNA and the metazoan cis-spliceosomal small nuclear RNAs...
- The Cdc45·Mcm2-7·GINS protein complex in trypanosomes regulates DNA replication and interacts with two Orc1-like proteins in the origin recognition complexHung Quang Dang
Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas 77030, USA
J Biol Chem 286:32424-35. 2011..All together, we identified the Cdc45·Mcm2-7·GINS complex as the replicative helicase that interacts with two Orc1-like proteins in the unusual origin recognition complex in trypanosomes...
- The 3' untranslated region of the hsp 70 genes maintains the level of steady state mRNA in Trypanosoma brucei upon heat shockM G Lee
Department of Pathology, New York University Medical Center, 550 First Avenue, New York, NY 10016, USA
Nucleic Acids Res 26:4025-33. 1998....
- Altering the reaction specificity of eukaryotic ornithine decarboxylaseL K Jackson
Department of Pharmacology and Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9041, USA
Biochemistry 39:11247-57. 2000..The results further provide insight into the mechanism by which this class of PLP-dependent enzymes controls reaction specificity...
- Pentatricopeptide repeat proteins stimulate mRNA adenylation/uridylation to activate mitochondrial translation in trypanosomesInna Aphasizheva
Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA
Mol Cell 42:106-17. 2011..Furthermore, we demonstrate that A/U-tailed mRNA preferentially interacts with the small ribosomal subunit, whereas edited substrates and complexes bind to the large subunit...
- Pentamidine movement across the murine blood-brain and blood-cerebrospinal fluid barriers: effect of trypanosome infection, combination therapy, P-glycoprotein, and multidrug resistance-associated proteinLisa Sanderson
King s College London, Pharmaceutical Sciences Research Division, Hodgkin Building, Guy s Campus, London, SE1 1UL, UK
J Pharmacol Exp Ther 329:967-77. 2009..However, coadministration of P-glycoprotein and/or MRP inhibitors with pentamidine or other diamidines might provide a means of improving efficacy against CNS stage HAT...
- Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcriptsE Ullu
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510 8056
Mol Cell Biol 13:720-5. 1993..Thus, trans splicing is cotranscriptional. In addition, we show that trans splicing precedes polyadenylation in the processing of trypanosome tubulin pre-mRNAs...
- Perturbation of phosphatidylethanolamine synthesis affects mitochondrial morphology and cell-cycle progression in procyclic-form Trypanosoma bruceiAita Signorell
Institute of Biochemistry and Molecular Medicine, University of Bern, 3012 Bern, Switzerland
Mol Microbiol 72:1068-79. 2009..In contrast, RNAi against choline-/ethanolamine phosphotransferase, which affected PC as well as PE levels, caused a cell division phenotype characterized by non-division of the nucleus and production of zoids...
- Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partnersTamara D da Costa Lima
Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhaes FIOCRUZ, Recife, PE, Brazil
Eukaryot Cell 9:1484-94. 2010..Our results indicate that these homologues have evolved divergent functions, some of which may be unique to the trypanosomatids, and reinforces a role for LmPABP1 in translation through its interaction with the eIF4G homologue...
- The trypanolytic factor-mechanism, impacts and applicationsRichard J Wheeler
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, UK
Trends Parasitol 26:457-64. 2010..b. brucei and T. b. rhodesiense. This has major implications for treatment and prevention of human and animal African trypanosomiasis...
- Histone H3 trimethylated at lysine 4 is enriched at probable transcription start sites in Trypanosoma bruceiJessica R Wright
Laboratory of Molecular Parasitology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
Mol Biochem Parasitol 172:141-4. 2010..H3K4me3 largely co-localizes with H4K10ac, but with a skew towards the upstream side of the H4K10ac peak, suggesting that it is a component of specific nucleosomes that play a role in Pol II transcription initiation...
- Defects in the N-linked oligosaccharide biosynthetic pathway in a Trypanosoma brucei glycosylation mutantAlvaro Acosta-Serrano
Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
Eukaryot Cell 3:255-63. 2004..The smaller OSL glycan in the ConA 1-1 explains how some procyclin polypeptides bear a Man4GlcNAc2 modified with a terminal N-acetyllactosamine group, which is poorly recognized by ConA...
- Small nucleolar RNA interference in Trypanosoma brucei: mechanism and utilization for elucidating the function of snoRNAsSachin Kumar Gupta
The Mina and Everard Goodman Faculty of Life Sciences and Advanced Materials and Nanotechnology Institute, Bar Ilan University, Ramat Gan 52900 Israel
Nucleic Acids Res 38:7236-47. 2010..snoRNAi of TB11Cs2C2 resulted in defects in generating srRNA-2 and LSUβ rRNA. This is the first snoRNA described so far to engage in trypanosome-specific processing events...
- Mechanisms of Resistance and Susceptibility to African Trypanosome InfectionStephen L Hajduk; Fiscal Year: 2013DESCRIPTION (provided by applicant): Trypanosoma brucei brucei causes Nagana in cattle but is non-infectious to humans because of its susceptibility to the cytotoxic activity of normal human serum...
- Mechanism of membrane permeabilization by a cytolytic high density lipoproteinJOHN MATTHEW HARRINGTON; Fiscal Year: 2010Humans exhibit an innate immunity against the mammalian parasite Trypanosoma brucei brucei, the causative agent of Nagana in African cattle. The morphologically indistinct T. brucei subspecies T. b. rhodesiense and T. b...
- Origin of Virulence Factors in African TrypanosomesANDREW MCARTHUR; Fiscal Year: 2004..brucei rhodesiense are protozoan parasites that cause African sleeping sickness in humans while Trypanosoma brucei brucei causes a wasting disease in cattle called Nagana...
- MITOCHONDRIAL ELECTRON TRANSPORT IN AFRICAN TRYPANOSOMESALLEN CLARKSON JR; Fiscal Year: 1993..b>Trypanosoma brucei brucei will be used as a model to achieve two primary objectives...
- LYSIS OF TRYPANOSOMA BRUCEI BY HUMAN SERUMStephen Tomlinson; Fiscal Year: 2000b>Trypanosoma brucei brucei, unlike the human pathogens Tau beta rhodesiense and Tau gambiense, is lysed by human serum...
- APOLIPOPROTEIN E ALLELES IN MODULATING TRYPANOSOMAGeorge Martin; Fiscal Year: 2001..In this proposal, we test a specific candidate organism, Trypanosoma brucei brucei, a protozoan that is the cause of endemic infections in many regions of Africa and is associated with a ..
- Zinc metalloprotease families in Trypansoma bruceiJOHN DONELSON; Fiscal Year: 2009....
- Software tools for analysis and visulaization of protein structureEthan A Merritt; Fiscal Year: 2010..The algorithms and computation tools developed as part of this work will be distributed freely, and we will provide web-based state of the art analysis of structures submitted by external researchers. ..
- MITOCHONDRIAL PROTEIN IMPORT IN AFRICAN TRYPANOSOMESMinu Chaudhuri; Fiscal Year: 2002..Understanding of this developmental process will elucidate one of the major aspects of trypanosomal parasitism. This in turn will help us to design rational trypanocidal chemotherapeutic strategies. ..
- EXPANDED PROTEIN STRUCTURE REFINEMENT AND VALIDATIONEthan Merritt; Fiscal Year: 2005..This, in turn, will make it easier to identify key biological features such as the presence and conformation of bound ligands, and the nature of hinge and inter-domain motions. ..
- AMASTIN/TUZIN GENE CLUSTER OF TRYPANOSOMA CRUZIJOHN DONELSON; Fiscal Year: 2001..Finally, we will assess whether amastin and/or tuzin can serve a target antigens for an improved diagnostic test for T. cruzi infection. ..
- GP46 GENES AND PROTEIN EXPRESSION IN LEISHMANIA CHAGASIJOHN DONELSON; Fiscal Year: 2002....
- Targeting Calpain for Novel Anticancer AgentsISAAC DONKOR; Fiscal Year: 2005..Additionally, the compounds are useful biomedical tools for investigating the intracellular roles of Calpain due to their metabolic stability. The compounds are also potential anticancer agents. ..
- RNA METABOLISM IN TRYPANOSOMESElisabetta Ullu; Fiscal Year: 2009..3. Further characterize the RNAi pathway in terms of its components and how the interaction of TbAGO1 with translating ribosomes is brought about. ..
- DEVELOPMENT OF GENETIC TOOLS FOR TRYPANOSOMA BRUCEIGEORGE CROSS; Fiscal Year: 2006..abstract_text> ..
- Water-Soluble and Metabolically Stable Calpain Inhibitors as CardioprotectantsISAAC DONKOR; Fiscal Year: 2008..Furthermore, the proposed studies will provide mechanistic insight into the mode of action of calpain inhibitors as cardioprotectants. ..
- Biochemical and Functional Analysis of TrypaninKENT HILL; Fiscal Year: 2007..Therefore, in addition to addressing fundamental questions in cell biology, this research directly impacts efforts to understand and treat infectious diseases and genetic diseases in humans. ..
- Biology of Host-Parasite Interactions 2008 Gordon Research ConferenceElisabetta Ullu; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- 2006 Biology of Host-Parasite Interactions Gordon Research ConferenceElisabetta Ullu; Fiscal Year: 2006..Host-Cell Invasion (Discussion Leader: Barbara Burleigh: Harvard) Trichomonads and Amoebae (Discussion Leader: Patricia Johnson, UCLA) Parasite Membrane Biology (Discussion Leader: Scott Landfear: University of Oregon) ..
- Telomere structure and function in Trypanosoma bruceiGEORGE CROSS; Fiscal Year: 2005..There are significant similarities between the telomeres of trypanosomes and humans, so trypanosomes would be a useful model in which to study events that are relevant to human aging and cancer. ..
- PROBING THE S' SUBSITES OF CALPAINISAAC DONKOR; Fiscal Year: 2002..Selected potent and cell permeable inhibitors will be tested in the rat isolated heart ischemia model for cardioprotective effect. ..
- A NOVEL MICROTUBULE-ASSOCIATED PROTEIN IN TRYPANOSOMESKENT HILL; Fiscal Year: 2002....
- Chemoprevention Potential of Calpain InhibitorsISAAC DONKOR; Fiscal Year: 2007..The proposal is significant and innovative because it seeks to investigate calpain as a pharmacological target for the discovery of a new class of chemoprevention agents. [unreadable] [unreadable] [unreadable]..
- RNA Uridylytransferases of TrypanosomesRuslan Aphasizhev; Fiscal Year: 2009..Sequences of the newly identified UTP-specific transferases will be used to establish characteristic protein motifs for this family of enzymes. ..
- Polyamine and glutathione metabolism in trypanosomes.Margaret Phillips; Fiscal Year: 2008..In Aim 3 we propose to study the effects of both genetic and metabolic perturbations in the pathway on the levels of key enzymes and metabolites. ..
- Antigenic variation in rickettsial transmissionGuy Palmer; Fiscal Year: 2008..This hypothesis will be addressed using comprehensive identification of the variant population in the reservoir host and tick vector and testing whether immune responses against a broad array of variants prevents superinfection. ..
- Inhibition of Alzheimer's Beta-Amyloid Fibril FormationDuy Hua; Fiscal Year: 2009..However, soluble oligomeric Abeta showed high neuronal toxicity. Inhibition of the formation of these toxic soluble Abeta oligomers would provide therapeutics for AD. ..
- Polyamine Metabolism in LeishmaniaBuddy Ullman; Fiscal Year: 2008..donovani. We propose, therefore, to ascertain whether the disparity in the abilities of ?lmarg L. mexicana and ?ldodc L. donovani to infect mice can be ascribed to species or gene differences. ..
- GPCR signaling cascades in Cryptococcus neoformansJoseph Heitman; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Role of Calcineurin in Fungal VirulenceJoseph Heitman; Fiscal Year: 2009..Importantly, this pathway can be targeted for therapeutic intervention using non-immunosuppressive calcineurin inhibitors that retain antifungal activity and synergistic drug combinations that we have discovered. ..
- Functional Characterization of Leishmania TransportersScott M Landfear; Fiscal Year: 2010..Overall, this proposal will provide a detailed biochemical and genetic dissection of glucose transporter function and the essential role of these permeases in the parasite life cycle. ..
- Genetic analysis of Cryptococcus neoformans virulenceJoseph Heitman; Fiscal Year: 2009..These studies will enable a genome-wide definition of the gene set contributing to virulence of this common human fungal pathogen. ..
- Transcription of protein-coding genes in LeishmaniaPETER JOHN MYLER; Fiscal Year: 2010..In this proposal, we will test this hypothesis and build on our current work to further characterize the chromatin-modifying enzymes that are involved in transcription initiation. ..
- PROTEIN PHOSPHORYLATION IN TRYPANOSOMESMarilyn Parsons; Fiscal Year: 2010..Together these studies will signficantly expand our knowledge of the network of signaling interactions in trypanosomes, with the goal of identifying new drug and vaccine targets. ..
- Structural studies on the P1 plasmid partition apparatusMaria Schumacher; Fiscal Year: 2009....
- Trypansome RNA Editing in Two Developmental FormsJorge Cruz Reyes; Fiscal Year: 2008..Together these studies should provide important insights into the developmental control of RNA editing, and understanding of relevant RNA/protein interactions during this unique maturation process. [unreadable] [unreadable]..
- 2007 Polyamines Gordon Conference and Graduate Research SeminarMargaret Phillips; Fiscal Year: 2007..abstract_text> ..
- Anaplasma - B. burgorferi/endothelijm interactionsDENNIS GRAB; Fiscal Year: 2006..burgdorferi. The elucidation of the steps that explain the enhancement may allow for more intensive basic and clinical investigation into mechanisms of virulence for Lyme disease, HGE, and coinfections. ..
- Conference on Drugs Against Protozoan ParasitesMargaret Phillips; Fiscal Year: 2005..Experts in drug development outside of parasitology have also been invited in an effort to stimulate their interests in tropical medicine and to learn from their experiences. ..
- GRNA/MRNA INTERACTIONS IN TRYPANSOME RNA EDITINGDONNA KOSLOWSKY; Fiscal Year: 2005..Understanding kRNA editing, which is unique to these organisms, will contribute to the fundamental knowledge of the parasite and may lead to the development of new strategies for disease intervention and control. ..
- Anti-Trypanosomal Agents from a Neotropical CloudforestWILLIAM SETZER; Fiscal Year: 2004..The long-term benefit of this research project is the development of new medicines to treat patients with Chagas disease. ..
- MOLECULAR ANALYSIS OF THE GLYCOSOME.Marilyn Parsons; Fiscal Year: 2004..Their studies are aimed at understanding glycosomal biogenesis and protein input and the relationship of these processes and the molecules involved to peroxisome biogenesis in the human host. ..
- Adaptation of ribozyme technology to LeishmaniaNANCY STURM; Fiscal Year: 2004..Once the parameters of the technique are established increasingly ambitious experiments can be designed including targeting of the spliced SL or discrete mRNAs. ..
- MITOCHONDRIAL RNA METABOLISM IN TRYPANOSOMA BRUCEILAURIE READ; Fiscal Year: 2004..Moreover, since little is known about RNA degradation in the mitochondria of any system, these studies will provide insights into the process of regulated mitochondrial RNA stability in higher organisms. ..
- SCAVENGER RECEPTOR OF AFRICAN TRYPANOSOMESJayne Raper; Fiscal Year: 2003..Raper's ability to pursue her long-term goal of elucidating trypanosome receptors and other invariant surface proteins as potential therapeutic targets. ..
- A CHEMICALLY SYNTHETIC ANTI-TOXIC VACCINE AGAINST MALARILouis Schofield; Fiscal Year: 2002..Clinical immunity to malaria is acquired considerably earlier than anti-parasite immunity, and it is possible that this is mediated by anti-toxin mechanisms. ..
- IMMUNOBIOLOGY OF MALARIA GLYCOSYLPHOSPHATIDYLINOSITOLLouis Schofield; Fiscal Year: 2001..These areas are central to understanding the host/parasite interaction and the development of effective vaccines to several protozoal pathogens. ..
- PPR family proteins in trypanosome mitochondriaLAURIE READ; Fiscal Year: 2006..Since the functions of PPRs are not well understood in general, they will likely provide novel insights into the functions of this conserved protein family in higher organisms as well. [unreadable] [unreadable]..
- Golgi Architecture, Biogenesis and FunctionElisabetta Ullu; Fiscal Year: 2007..Both have a single Golgi apparatus, the duplication of which can be followed in real time using video fluorescence microscopy. These simple systems are also medically important. [unreadable] [unreadable]..
- GENETIC ANALYSIS OF PURINE METABOLISM IN LEISHMANIA DONOBuddy Ullman; Fiscal Year: 2007..coli that overexpress hgprt genes, and[unreadable] intact parasites. ..
- Transcription and Insertional editing in PhysarumJonatha Gott; Fiscal Year: 2006..abstract_text> ..
- Nucleoside Transporters of Plasmodium falciparumBuddy Ullman; Fiscal Year: 2007..falciparum in order to test whether PfNT1 function is essential to the intact parasite. We will then characterize the resultant transport and growth phenotypes. ..
- R13 travel grant for Polyamine/parasite conference in Portland, ORBuddy Ullman; Fiscal Year: 2006..abstract_text> ..
- MEMBRANE FUSION ATPASES AND THE GOLGI APPARATUSAyano Satoh; Fiscal Year: 2007..Insights into the molecular mechanism of Golgi reassembly may therefore provide insight into these medical conditions. ..
- MITOCHONDRIAL RNA METABOLISM IN TRYPANOSOMA BRUCEILAURIE READ; Fiscal Year: 2007..Proteins that specifically bind the RPS12 stabilization determinant will be identified. 3) Determine the structure and function of the mitochondrial degradosome using a combined affinity chromatography and RNAi approach. ..
- Genome Evolution, Innovation and Adaptation in the ApicomplexaJessica C Kissinger; Fiscal Year: 2010..The results of these studies will be shared with the larger research community via publication and deposition in community databases and Bioinformatics Resource Centers. ..
- A High Throughput Assay to Identify Onhibitors of Trypanosome RNA Editing LigaseNancy Ernst; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Postsynthetic Modifications of Bacterial Membrane LipidsJohn Cronan; Fiscal Year: 2006..These processes will be studied by a combination of biochemical, genetic, and molecular biological approaches including tests of pathogenesis in a mouse model system ..
- Enzyme Targets-Sterol Synthesis-Opportunistic PathogensWILLIAM NES; Fiscal Year: 2004..The results from these studies will facilitate the design of therapeutic strategies aimed to provide species-specific discrimination of inhibition in the ergosterol biosynthetic pathway. ..
- Sir2 regulation and chemical modulationAnthony A Sauve; Fiscal Year: 2010..abstract_text> ..
- Trypanosomatid Mitochondrial DNA PolymerasesMichele Klingbeil; Fiscal Year: 2008..The T. brucei pol I-like protein family also offers the unique opportunity to uncover insights into the evolutionary strategies used to produce a replicative polymerase. ..
- The SMD-Relaxed Complex Method for Drug DesignRommie Amaro; Fiscal Year: 2008..The success of this new approach will be demonstrated on an essential RNA editing enzyme found in the trypanosomatid pathogens, which are responsible for several devastating tropical diseases. [unreadable] [unreadable] [unreadable]..
- Ecology, genetics and physiology of insect vectorsElliot Krafsur; Fiscal Year: 2006..abstract_text> ..
- Topoisomerases: Target for Antitrypanosomal TherapyTheresa Shapiro; Fiscal Year: 2006..Compounds that appear most promising will be evaluated in mice. These studies take a multi-faceted, rational, and tangible approach to the development of much-needed new anti-trypanosomal chemotherapy. ..