drosophila

Summary

Summary: A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.

Top Publications

  1. pmc FlyBase: enhancing Drosophila Gene Ontology annotations
    Susan Tweedie
    Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
    Nucleic Acids Res 37:D555-9. 2009
  2. ncbi Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
    A H Brand
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
    Development 118:401-15. 1993
  3. pmc Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease
    Scott J Gratz
    Genetics Training Program, University of Wisconsin, Madison, Madison, WI 53706, USA
    Genetics 194:1029-35. 2013
  4. ncbi Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis
    T Lee
    Department of Biological Sciences, Stanford University, California 94305, USA
    Neuron 22:451-61. 1999
  5. ncbi The dorsoventral regulatory gene cassette spätzle/Toll/cactus controls the potent antifungal response in Drosophila adults
    B Lemaitre
    Institut de Biologie Moléculaire at Cellulaire, UPR 9022 du Centre National de la Recherche Scientifique, Strasbourg, France
    Cell 86:973-83. 1996
  6. ncbi Spatiotemporal rescue of memory dysfunction in Drosophila
    Sean E McGuire
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 302:1765-8. 2003
  7. pmc Elucidation of a universal size-control mechanism in Drosophila and mammals
    Jixin Dong
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 130:1120-33. 2007
  8. ncbi A human homologue of the Drosophila Toll protein signals activation of adaptive immunity
    R Medzhitov
    Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520 8011, USA
    Nature 388:394-7. 1997
  9. pmc Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system
    Andrew R Bassett
    Medical Research Council Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, OX1 3PT, UK
    Cell Rep 4:220-8. 2013
  10. ncbi The Hippo signaling pathway coordinately regulates cell proliferation and apoptosis by inactivating Yorkie, the Drosophila Homolog of YAP
    Jianbin Huang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Cell 122:421-34. 2005

Research Grants

Detail Information

Publications361 found, 100 shown here

  1. pmc FlyBase: enhancing Drosophila Gene Ontology annotations
    Susan Tweedie
    Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
    Nucleic Acids Res 37:D555-9. 2009
    FlyBase (http://flybase.org) is a database of Drosophila genetic and genomic information...
  2. ncbi Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
    A H Brand
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
    Development 118:401-15. 1993
    ..The gene encoding the yeast transcriptional activator GAL4 is inserted randomly into the Drosophila genome to drive GAL4 expression from one of a diverse array of genomic enhancers...
  3. pmc Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease
    Scott J Gratz
    Genetics Training Program, University of Wisconsin, Madison, Madison, WI 53706, USA
    Genetics 194:1029-35. 2013
    We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline...
  4. ncbi Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis
    T Lee
    Department of Biological Sciences, Stanford University, California 94305, USA
    Neuron 22:451-61. 1999
    We describe a genetic mosaic system in Drosophila, in which a dominant repressor of a cell marker is placed in trans to a mutant gene of interest...
  5. ncbi The dorsoventral regulatory gene cassette spätzle/Toll/cactus controls the potent antifungal response in Drosophila adults
    B Lemaitre
    Institut de Biologie Moléculaire at Cellulaire, UPR 9022 du Centre National de la Recherche Scientifique, Strasbourg, France
    Cell 86:973-83. 1996
    The cytokine-induced activation cascade of NF-kappaB in mammals and the activation of the morphogen dorsal in Drosophila embryos show striking structural and functional similarities (Toll/IL-1, Cactus/I-kappaB, and dorsal/NF-kappaB)...
  6. ncbi Spatiotemporal rescue of memory dysfunction in Drosophila
    Sean E McGuire
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 302:1765-8. 2003
    We have developed a method for temporal and regional gene expression targeting (TARGET) in Drosophila and show the simultaneous spatial and temporal rescue of a memory defect...
  7. pmc Elucidation of a universal size-control mechanism in Drosophila and mammals
    Jixin Dong
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 130:1120-33. 2007
    ..In Drosophila, these two processes are orchestrated by the Hippo kinase cascade, a growth-suppressive pathway that ultimately ..
  8. ncbi A human homologue of the Drosophila Toll protein signals activation of adaptive immunity
    R Medzhitov
    Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520 8011, USA
    Nature 388:394-7. 1997
    ..We report here the cloning and characterization of a human homologue of the Drosophila toll protein (Toll) which has been shown to induce the innate immune response in adult Drosophila...
  9. pmc Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system
    Andrew R Bassett
    Medical Research Council Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, OX1 3PT, UK
    Cell Rep 4:220-8. 2013
    Here, we present a simple and highly efficient method for generating and detecting mutations of any gene in Drosophila melanogaster through the use of the CRISPR/Cas9 system (clustered regularly interspaced palindromic repeats/..
  10. ncbi The Hippo signaling pathway coordinately regulates cell proliferation and apoptosis by inactivating Yorkie, the Drosophila Homolog of YAP
    Jianbin Huang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Cell 122:421-34. 2005
    ..In Drosophila, these two processes are regulated by a pathway involving the Ste20-like kinase Hippo (Hpo) and the NDR family ..
  11. ncbi Adaptive protein evolution at the Adh locus in Drosophila
    J H McDonald
    Department of Ecology and Evolutionary Biology, Princeton University, New Jersey 08544
    Nature 351:652-4. 1991
    ..DNA sequence data on the Adh locus (encoding alcohol dehydrogenase, EC 1.1.1.1) in three species in the Drosophila melanogaster species subgroup do not fit this expectation; instead, there are more fixed replacement differences ..
  12. ncbi Hsp90 as a capacitor for morphological evolution
    S L Rutherford
    Howard Hughes Medical Institute, University of Chicago, Illinois 60637, USA
    Nature 396:336-42. 1998
    ..We report here that when Drosophila Hsp90 is mutant or pharmacologically impaired, phenotypic variation affecting nearly any adult structure is ..
  13. pmc Regulated functional alternative splicing in Drosophila
    Julian P Venables
    Universite Montpellier 2, UMR 5535, Institut de Genetique Moleculaire de Montpellier, CNRS, 1919 Route de Mende, 34293 Montpellier Cedex 05, France
    Nucleic Acids Res 40:1-10. 2012
    ..splicing expands the coding capacity of metazoan genes, and it was largely genetic studies in the fruit-fly Drosophila melanogaster that established the principle that regulated alternative splicing results in tissue- and stage-..
  14. pmc Multilocus methods for estimating population sizes, migration rates and divergence time, with applications to the divergence of Drosophila pseudoobscura and D. persimilis
    Jody Hey
    Department of Genetics, Rutgers, State University of New Jersey, Piscataway, New Jersey 08854, USA
    Genetics 167:747-60. 2004
    ..These methods are applied to a large multilocus data set from Drosophila pseudoobscura and D. persimilis. The species are estimated to have diverged approximately 500,000 years ago...
  15. ncbi Hedgehog signaling in development and cancer
    Jin Jiang
    Department of Developmental Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    Dev Cell 15:801-12. 2008
    ..We discuss how the Hh pathway has diverged during evolution and how it integrates with other signaling pathways to control cell growth and patterning...
  16. pmc Variant ionotropic glutamate receptors as chemosensory receptors in Drosophila
    Richard Benton
    Laboratory of Neurogenetics and Behavior, The Rockefeller University, 1230 York Avenue, Box 63, New York, NY 10065, USA
    Cell 136:149-62. 2009
    ..We have characterized a family of iGluR-related genes in Drosophila, which we name ionotropic receptors (IRs)...
  17. ncbi Ablation of insulin-producing neurons in flies: growth and diabetic phenotypes
    Eric J Rulifson
    Department of Developmental Biology, Beckman Center B300, Stanford University, Stanford, CA 94305 5329, USA
    Science 296:1118-20. 2002
    In the fruit fly Drosophila, four insulin genes are coexpressed in small clusters of cells [insulin-producing cells (IPCs)] in the brain...
  18. pmc Molecular evolution and functional characterization of Drosophila insulin-like peptides
    Sebastian Gronke
    Institute of Healthy Ageing, Department of Genetics, Evolution, and Environment, University College London, London, United Kingdom
    PLoS Genet 6:e1000857. 2010
    ..Invertebrate genomes often contain multiple genes encoding insulin-like ligands, including seven Drosophila insulin-like peptides (DILPs)...
  19. ncbi Homeobox genes and axial patterning
    W McGinnis
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511
    Cell 68:283-302. 1992
  20. pmc Recruitment of human muscleblind proteins to (CUG)(n) expansions associated with myotonic dystrophy
    J W Miller
    Department of Molecular Genetics and Microbiology, Centers for Gene Therapy and Mammalian Genetics, University of Florida College of Medicine, Gainesville, FL 32610, USA
    EMBO J 19:4439-48. 2000
    ..Remarkably, the EXP proteins are homologous to the Drosophila muscleblind proteins required for terminal differentiation of muscle and photoreceptor cells...
  21. pmc Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila
    Zhongsheng Yu
    State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Datun Road 15, Beijing 100101, China
    Genetics 195:289-91. 2013
    We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently...
  22. ncbi Drosophila intestinal response to bacterial infection: activation of host defense and stem cell proliferation
    Nicolas Buchon
    Global Health Institute, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    Cell Host Microbe 5:200-11. 2009
    Although Drosophila systemic immunity is extensively studied, little is known about the fly's intestine-specific responses to bacterial infection...
  23. ncbi Efficient and specific modifications of the Drosophila genome by means of an easy TALEN strategy
    Jiyong Liu
    State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, The Chinese Academy of Sciences, Datun Road 15, Beijing 100101, China
    J Genet Genomics 39:209-15. 2012
    ..have, step by step, developed powerful genetic tools for manipulating and functionally dissecting the Drosophila genome, but room for improving these technologies and developing new techniques is still large, especially today ..
  24. ncbi Histone methyltransferase activity of a Drosophila Polycomb group repressor complex
    Jurg Muller
    EMBL, Gene Expression Programme, Meyerhofstr 1, 69117 Heidelberg, Germany
    Cell 111:197-208. 2002
    ..A SET domain in E(Z) suggests this complex might methylate histones. We purified an ESC-E(Z) complex from Drosophila embryos and found four major subunits: ESC, E(Z), NURF-55, and the PcG repressor, SU(Z)12...
  25. pmc Analysis of variation at transcription factor binding sites in Drosophila and humans
    Mikhail Spivakov
    European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SD, UK
    Genome Biol 13:R49. 2012
    ..investigate TFBS variability by combining transcription factor binding maps generated by ENCODE, modENCODE, our previously published data and other sources with genomic variation data for human individuals and Drosophila isogenic lines.
  26. pmc A fructose receptor functions as a nutrient sensor in the Drosophila brain
    Tetsuya Miyamoto
    Department of Molecular and Cellular Medicine, Texas A and M Health Science Center, College Station, TX 77845, USA
    Cell 151:1113-25. 2012
    ..Thus, our studies indicate that the Gr43a-expressing brain neurons function as a nutrient sensor for hemolymph fructose and assign opposing valence to feeding experiences in a satiation-dependent manner...
  27. pmc Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut
    Huaqi Jiang
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA
    Cell 137:1343-55. 2009
    ..We show here that when enterocytes (ECs) in the Drosophila midgut are subjected to apoptosis, enteric infection, or JNK-mediated stress signaling, they produce cytokines (..
  28. ncbi Mosaic analysis with a repressible cell marker (MARCM) for Drosophila neural development
    T Lee
    Department of Cell and Structural Biology, University of Illinois, Urbana Champaign, Urbana, IL 61801, USA
    Trends Neurosci 24:251-4. 2001
    ..mosaic system to label either neurons derived from a common progenitor or isolated single neurons, in the Drosophila CNS...
  29. ncbi Role for a bidentate ribonuclease in the initiation step of RNA interference
    E Bernstein
    Cold Spring Harbor Laboratory, New York 11724, USA
    Nature 409:363-6. 2001
    ..The enzyme has a distinctive structure, which includes a helicase domain and dual RNase III motifs. Dicer also contains a region of homology to the RDE1/QDE2/ARGONAUTE family that has been genetically linked to RNAi...
  30. ncbi Evolution of genes and genomes on the Drosophila phylogeny
    Andrew G Clark
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Nature 450:203-18. 2007
    ..The genomes of 12 Drosophila species, ten of which are presented here for the first time (sechellia, simulans, yakuba, erecta, ananassae, ..
  31. ncbi A conserved dedicated olfactory circuit for detecting harmful microbes in Drosophila
    Marcus C Stensmyr
    Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans Knoll Strasse 8, 07745 Jena, Germany
    Cell 151:1345-57. 2012
    Flies, like all animals, need to find suitable and safe food. Because the principal food source for Drosophila melanogaster is yeast growing on fermenting fruit, flies need to distinguish fruit with safe yeast from yeast covered with ..
  32. ncbi Analysis of genetic mosaics in developing and adult Drosophila tissues
    T Xu
    Howard Hughes Medical Institute, University of California, Berkeley 94720
    Development 117:1223-37. 1993
    ..the generation and analysis of clones of genetically distinct cells in developing and adult tissues of Drosophila. Each of these strains carries an FRT element, the target for the yeast FLP recombinase, near the base of a ..
  33. ncbi A Drosophila model of FUS-related neurodegeneration reveals genetic interaction between FUS and TDP-43
    Nicholas A Lanson
    Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
    Hum Mol Genet 20:2510-23. 2011
    ..Ectopic expression of human ALS-causing FUS/TLS mutations in Drosophila caused an accumulation of ubiquitinated proteins, neurodegeneration, larval-crawling defect and early lethality...
  34. ncbi An evolutionarily conserved function of the Drosophila insulin receptor and insulin-like peptides in growth control
    W Brogiolo
    Zoologisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057, Zurich, Switzerland
    Curr Biol 11:213-21. 2001
    ..Studies in Drosophila have identified an evolutionarily conserved signaling pathway that regulates organismal size and that includes ..
  35. ncbi Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites
    Birgit Czermin
    Adolf Butenandt Institut, University of Munich, Schillerstrasse 44, 80336 Munich, Germany
    Cell 111:185-96. 2002
    ..Histone H3 methylated in vitro by the E(Z)/ESC complex binds specifically to Polycomb protein...
  36. pmc A comprehensive map of insulator elements for the Drosophila genome
    Nicolas Negre
    Institute for Genomics and Systems Biology, Department of Human Genetics, and Department of Ecology and Evolution, University of Chicago, Chicago, Illinois, USA
    PLoS Genet 6:e1000814. 2010
    ..CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF-to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined insulators...
  37. ncbi Morphological and molecular characterization of adult midgut compartmentalization in Drosophila
    Nicolas Buchon
    Global Health Institute, School of Life Sciences, Station 19, EPFL, 1015 Lausanne, Switzerland
    Cell Rep 3:1725-38. 2013
    ..Here, we provide a comprehensive atlas of Drosophila adult midgut...
  38. pmc Roles of the Drosophila LRRK2 homolog in Rab7-dependent lysosomal positioning
    Mark W Dodson
    Department of Neurology, The David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Hum Mol Genet 21:1350-63. 2012
    ..Here, we show that the Drosophila homolog of LRRK2 (Lrrk) localizes to the membranes of late endosomes and lysosomes, physically interacts with ..
  39. pmc High sugar-induced insulin resistance in Drosophila relies on the lipocalin Neural Lazarillo
    Matthieu Y Pasco
    Institute of Biology Valrose iBV, CNRS UMR 7707, Inserm UMR 1091, University of Nice Sophia Antipolis, Nice, France
    PLoS ONE 7:e36583. 2012
    ..In organisms with simpler IIS, this functional separation is questionable. In Drosophila IIS consists of several insulin-like peptides called Dilps, activating a unique membrane receptor and its ..
  40. pmc Contact chemoreceptors mediate male-male repulsion and male-female attraction during Drosophila courtship
    Robert Thistle
    Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute, 16 Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 149:1140-51. 2012
    The elaborate courtship ritual of Drosophila males is dictated by neural circuitry established by the transcription factor Fruitless and triggered by sex-specific sensory cues...
  41. pmc Traces of Drosophila memory
    Ronald L Davis
    Department of Neuroscience, The Scripps Research Institute Florida, Jupiter, FL 33410, USA
    Neuron 70:8-19. 2011
    ....
  42. ncbi Polycomb-dependent regulatory contacts between distant Hox loci in Drosophila
    Frédéric Bantignies
    Institut de Genetique Humaine, CNRS UPR 1142, Montpellier, France
    Cell 144:214-26. 2011
    In Drosophila melanogaster, Hox genes are organized in an anterior and a posterior cluster, called Antennapedia complex and bithorax complex, located on the same chromosome arm and separated by 10 Mb of DNA...
  43. ncbi Functional siRNAs and miRNAs exhibit strand bias
    Anastasia Khvorova
    Amgen, Inc, One Amgen Center Drive, Thousand Oaks, CA 91320, USA
    Cell 115:209-16. 2003
    ..Together, these results suggest that the thermodynamic properties of siRNA play a critical role in determining the molecule's function and longevity, possibly biasing the steps involved in duplex unwinding and strand retention by RISC...
  44. pmc Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi
    Ralph A Neumüller
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3, 1030 Vienna, Austria
    Cell Stem Cell 8:580-93. 2011
    ..we use genome-wide transgenic RNAi to identify 620 genes potentially involved in controlling this balance in Drosophila neuroblasts...
  45. pmc Hitchhiking under positive Darwinian selection
    J C Fay
    Committee on Genetics, University of Chicago, Chicago, Illinois 60637, USA
    Genetics 155:1405-13. 2000
    ..Application of the H test to published surveys of Drosophila variation reveals an excess of high frequency variants that are likely to have been influenced by positive ..
  46. pmc Effects of diet and development on the Drosophila lipidome
    Maria Carvalho
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Mol Syst Biol 8:600. 2012
    ..Furthermore, Drosophila differentially regulates uptake, mobilization and tissue accumulation of specific sterols, and undergoes ..
  47. pmc Drosophila ATM and ATR have distinct activities in the regulation of meiotic DNA damage and repair
    Eric F Joyce
    Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ 08854, USA
    J Cell Biol 195:359-67. 2011
    ..In this paper, we show that ATM and ATR have unique roles in the repair of meiotic DSBs in Drosophila melanogaster. ATR mutant analysis indicated that it is required for checkpoint activity, whereas ATM may not be...
  48. ncbi Phylogenetic perspectives in innate immunity
    J A Hoffmann
    Institute of Molecular and Cellular Biology, CNRS, Strasbourg, 67084, France
    Science 284:1313-8. 1999
    ..similarities between pathogen recognition, signaling pathways, and effector mechanisms of innate immunity in Drosophila and mammals, pointing to a common ancestry of these defenses...
  49. pmc The PINK1/Parkin pathway regulates mitochondrial morphology
    Angela C Poole
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 105:1638-43. 2008
    ..Genetic studies in Drosophila indicate that PINK1 acts upstream of Parkin in a common pathway that influences mitochondrial integrity in a ..
  50. ncbi Drosophila CENH3 is sufficient for centromere formation
    María José Mendiburo
    Max Planck Institute of Immunobiology, Stubeweg 51, 79108 Freiburg, Germany
    Science 334:686-90. 2011
    ..To address this question, we artificially targeted Drosophila CENH3 (CENP-A/CID) as a CID-GFP-LacI fusion protein to stably integrated lac operator (lacO) arrays...
  51. ncbi A novel family of divergent seven-transmembrane proteins: candidate odorant receptors in Drosophila
    P J Clyne
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Neuron 22:327-38. 1999
    ..We have developed a novel search algorithm, used it to search the Drosophila genomic sequence database, and identified a large multigene family encoding seven transmembrane domain proteins ..
  52. pmc Shadow enhancers foster robustness of Drosophila gastrulation
    Michael W Perry
    Department of Integrative Biology, University of California Berkeley, CA 94720, USA
    Curr Biol 20:1562-7. 2010
    ..These results suggest that shadow enhancers represent a novel mechanism of canalization whereby complex developmental processes "bring about one definite end-result regardless of minor variations in conditions" [6]...
  53. ncbi Proneural genes and the specification of neural cell types
    Nicolas Bertrand
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM Universite Louis Pasteur, B P 163, 67404 Illkirch Cedex, C U de Strasbourg, France
    Nat Rev Neurosci 3:517-30. 2002
    ..Parallel studies in Drosophila and vertebrates have revealed that proneural genes are key regulators of neurogenesis, coordinating the ..
  54. pmc Sorting of Drosophila small silencing RNAs partitions microRNA* strands into the RNA interference pathway
    Megha Ghildiyal
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    RNA 16:43-56. 2010
    ..Here, we show that while most Drosophila miRNAs are bound to Ago1, miRNA* strands accumulate bound to Ago2...
  55. ncbi Complementary function and integrated wiring of the evolutionarily distinct Drosophila olfactory subsystems
    Ana F Silbering
    Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, CH 1015 Lausanne, Switzerland
    J Neurosci 31:13357-75. 2011
    ..We have addressed these questions through comprehensive comparative analysis of the Drosophila olfactory subsystems that express the ionotropic receptors (IRs) and odorant receptors (ORs)...
  56. ncbi Conditional modification of behavior in Drosophila by targeted expression of a temperature-sensitive shibire allele in defined neurons
    T Kitamoto
    Division of Neurosciences, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, California 91010, USA
    J Neurobiol 47:81-92. 2001
    ..I introduce a novel and general method to conditionally perturb anatomically defined neurons in intact Drosophila. In this method, a temperature-sensitive allele of shibire (shi(ts1)) is overexpressed in neuronal subsets using ..
  57. ncbi Nutrient-dependent expression of insulin-like peptides from neuroendocrine cells in the CNS contributes to growth regulation in Drosophila
    Tomoatsu Ikeya
    Zoologisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057, Zurich, Switzerland
    Curr Biol 12:1293-300. 2002
    The insulin/IGF-1 signaling pathway controls cellular and organismal growth in many multicellular organisms. In Drosophila, genetic defects in components of the insulin signaling pathway produce small flies that are delayed in development ..
  58. ncbi Gene regulatory networks in the evolution and development of the heart
    Eric N Olson
    Department of Molecular Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    Science 313:1922-7. 2006
    ..The consequences of such mutations reveal the logic of organogenesis and the evolutionary origins of morphological complexity...
  59. ncbi Epigenetic regulation of cellular memory by the Polycomb and Trithorax group proteins
    Leonie Ringrose
    ZMBH, University of Heidelberg, 69120 Heidelberg, Germany
    Annu Rev Genet 38:413-43. 2004
    ..Here we review current ideas on the protein and DNA components of this transcriptional memory system and how they interact dynamically with each other to orchestrate cellular memory for several hundred genes...
  60. pmc Mitochondrial pathology and apoptotic muscle degeneration in Drosophila parkin mutants
    Jessica C Greene
    Department of Genome Sciences, University of Washington, P O Box 357730, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 100:4078-83. 2003
    ..To gain insight into the molecular mechanism responsible for selective cell death in AR-JP, we have created a Drosophila model of this disorder...
  61. pmc Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases
    Marina Bibikova
    Department of Biochemistry, University of Utah School of Medicine, Medical Research and Education Building, 20 North 1900 East, Salt Lake City, UT 84132, USA
    Genetics 161:1169-75. 2002
    ..We have tested this idea by designing a pair of ZFNs that recognize a unique site in the yellow (y) gene of Drosophila. When these nucleases were expressed in developing larvae, they led to somatic mutations specifically in the y ..
  62. pmc Chromosomal inversions and the reproductive isolation of species
    M A Noor
    Department of Biological Sciences, Life Sciences Building, Louisiana State University, Baton Rouge, LA 70803, USA
    Proc Natl Acad Sci U S A 98:12084-8. 2001
    ..We have examined the genetic basis of hybrid sterility and female species preferences in Drosophila pseudoobscura and Drosophila persimilis, two occasionally hybridizing North American species...
  63. pmc The carnegie protein trap library: a versatile tool for Drosophila developmental studies
    Michael Buszczak
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21218, USA
    Genetics 175:1505-31. 2007
    ..Using transposon mutagenesis in Drosophila, we constructed a library of 7404 protein trap and enhancer trap lines, the Carnegie collection, to facilitate ..
  64. pmc Gene family evolution across 12 Drosophila genomes
    Matthew W Hahn
    Department of Biology, Indiana University, Bloomington, Indiana, United States of America
    PLoS Genet 3:e197. 2007
    ..Here we use the genomes of 12 fully sequenced Drosophila species to study the gain and loss of genes at unprecedented resolution...
  65. ncbi Apoptotic cells can induce compensatory cell proliferation through the JNK and the Wingless signaling pathways
    Hyung Don Ryoo
    Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 252, New York, NY 10021, USA
    Dev Cell 7:491-501. 2004
    In many metazoans, damaged and potentially dangerous cells are rapidly eliminated by apoptosis. In Drosophila, this is often compensated for by extraproliferation of neighboring cells, which allows the organism to tolerate considerable ..
  66. ncbi Drosophila microbiome modulates host developmental and metabolic homeostasis via insulin signaling
    Seung Chul Shin
    School of Biological Science, Seoul National University and National Creative Research Initiative Center for Symbiosystem, Seoul 151 742, South Korea
    Science 334:670-4. 2011
    ..of a commensal bacterium, Acetobacter pomorum, modulates insulin/insulin-like growth factor signaling (IIS) in Drosophila to regulate host homeostatic programs controlling developmental rate, body size, energy metabolism, and ..
  67. pmc Sex-specific adaptation drives early sex chromosome evolution in Drosophila
    Qi Zhou
    Department of Integrative Biology, University of California Berkeley, Berkeley, CA 94720, USA
    Science 337:341-5. 2012
    ..We studied the sex chromosomes of Drosophila miranda, where a neo-Y chromosome originated only approximately 1 million years ago...
  68. ncbi Flies without centrioles
    Renata Basto
    The Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, UK
    Cell 125:1375-86. 2006
    ..The Drosophila protein DSas-4 is related to the human microcephaly protein CenpJ and the C. elegans centriolar protein Sas-4...
  69. pmc The ALS-associated proteins FUS and TDP-43 function together to affect Drosophila locomotion and life span
    Ji Wu Wang
    Center for Motor Neuron Biology and Disease, Columbia University Medical Center, New York, New York 10032, USA
    J Clin Invest 121:4118-26. 2011
    ..Here, we report that Drosophila mutants in which the homolog of FUS is disrupted exhibit decreased adult viability, diminished locomotor speed, ..
  70. ncbi New genes in Drosophila quickly become essential
    Sidi Chen
    Department of Ecology and Evolution, The University of Chicago, 1101 East 57th Street, Chicago, IL 60637, USA
    Science 330:1682-5. 2010
    ..we identified and phenotyped 195 young protein-coding genes, which originated 3 to 35 million years ago in Drosophila. Knocking down expression with RNA interference showed that 30% of newly arisen genes are essential for ..
  71. ncbi Molecular mechanisms of metabolic regulation by insulin in Drosophila
    Aurelio A Teleman
    German Cancer Research Center Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Biochem J 425:13-26. 2010
    The insulin signalling pathway is highly conserved from mammals to Drosophila. Insulin signalling in the fly, as in mammals, regulates a number of physiological functions, including carbohydrate and lipid metabolism, tissue growth and ..
  72. pmc Binding site turnover produces pervasive quantitative changes in transcription factor binding between closely related Drosophila species
    Robert K Bradley
    Department of Mathematics, University of California Berkeley, Berkeley, California, United States of America
    PLoS Biol 8:e1000343. 2010
    ..factors that initiate segmentation along the anterior-posterior axis in embryos of two closely related species: Drosophila melanogaster and Drosophila yakuba...
  73. ncbi Drosophila innate immunity: an evolutionary perspective
    Jules A Hoffmann
    CNRS, Institute of Molecular and Cellular Biology, 15 rue Descartes, 67000 Strasbourg, France
    Nat Immunol 3:121-6. 2002
    In response to microbial infections, Drosophila mounts a multifaceted immune response involving humoral reactions that culminate in the destruction of invading organisms by lytic peptides...
  74. ncbi Senseless, a Zn finger transcription factor, is necessary and sufficient for sensory organ development in Drosophila
    R Nolo
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Cell 102:349-62. 2000
    ..required for proper development of most cell types of the embryonic and adult peripheral nervous system (PNS) of Drosophila. Sens is a nuclear protein with four Zn fingers that is expressed and required in the sensory organ precursors (..
  75. pmc A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewal
    D N Cox
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710 USA
    Genes Dev 12:3715-27. 1998
    ..We cloned and characterized the Drosophila piwi gene and showed that it is required for the asymmetric division of GSCs to produce and maintain a daughter ..
  76. ncbi Three-dimensional reconstruction of brain-wide wiring networks in Drosophila at single-cell resolution
    Ann Shyn Chiang
    Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan, R O C
    Curr Biol 21:1-11. 2011
    ..Comprehensive brain wiring maps are thus needed in order to formulate hypotheses about information flow and also to guide genetic manipulations aimed at understanding how genes and circuits orchestrate complex behaviors...
  77. ncbi A direct role for dual oxidase in Drosophila gut immunity
    Eun Mi Ha
    Division of Molecular Life Science and Center for Cell Signaling Research, Ewha Woman s University, Seoul 120 750, South Korea
    Science 310:847-50. 2005
    ..Here, we show that the Drosophila nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme, dual oxidase (dDuox), is indispensable for ..
  78. pmc DroID 2011: a comprehensive, integrated resource for protein, transcription factor, RNA and gene interactions for Drosophila
    Thilakam Murali
    Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, Michigan, USA
    Nucleic Acids Res 39:D736-43. 2011
    DroID (http://droidb.org/), the Drosophila Interactions Database, is a comprehensive public resource for Drosophila gene and protein interactions...
  79. pmc Sex chromosome-specific regulation in the Drosophila male germline but little evidence for chromosomal dosage compensation or meiotic inactivation
    Colin D Meiklejohn
    Department of Biology, University of Rochester, Rochester, New York, United States of America
    PLoS Biol 9:e1001126. 2011
    ..How the X chromosome is regulated in the Drosophila melanogaster male germline is unclear...
  80. pmc The role of anorexia in resistance and tolerance to infections in Drosophila
    Janelle S Ayres
    Department of Microbiology and Immunology, Stanford University, Stanford, California, USA
    PLoS Biol 7:e1000150. 2009
    ..This suggests that attempts to extend lifespan through diet restriction or the manipulation of pathways mimicking this process will have complicated effects on a host's ability to fight infections...
  81. pmc Bacterial communities of diverse Drosophila species: ecological context of a host-microbe model system
    James Angus Chandler
    Center for Population Biology, Department of Evolution and Ecology, University of California Davis, Davis, California, USA
    PLoS Genet 7:e1002272. 2011
    b>Drosophila melanogaster is emerging as an important model of non-pathogenic host-microbe interactions. The genetic and experimental tractability of Drosophila has led to significant gains in our understanding of animal-microbial symbiosis...
  82. ncbi The birth and death of microRNA genes in Drosophila
    Jian Lu
    Department of Ecology and Evolution, University of Chicago, 1101 East 57th Street, Chicago, Illinois 60637, USA
    Nat Genet 40:351-5. 2008
    ..We carried out deep sequencing of miRNAs from three species of Drosophila, and obtained 107,000 sequences that map to no fewer than 300 loci that were not previously known...
  83. ncbi The role of site accessibility in microRNA target recognition
    Michael Kertesz
    Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel
    Nat Genet 39:1278-84. 2007
    ..Our study thus demonstrates that target accessibility is a critical factor in microRNA function...
  84. pmc Pervasive natural selection in the Drosophila genome?
    Guy Sella
    Department of Evolution, Systematics and Ecology, The Hebrew University, Jerusalem, Israel
    PLoS Genet 5:e1000495. 2009
    ..Recent evidence from Drosophila, reviewed here, suggests that this view may be invalid...
  85. ncbi Parallels between tissue repair and embryo morphogenesis
    Paul Martin
    Department of Physiology, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Development 131:3021-34. 2004
    ..to repair an embryonic epithelial wound as is involved during the morphogenetic episodes of dorsal closure in Drosophila and eyelid fusion in the mouse foetus...
  86. ncbi Molecular, anatomical, and functional organization of the Drosophila olfactory system
    Africa Couto
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3 5 A 1030 Vienna, Austria
    Curr Biol 15:1535-47. 2005
    ..A comprehensive understanding of the molecular and neural mechanisms of odor detection and processing requires complete maps of odorant receptor (Or) expression and ORN connectivity, preferably at single-cell resolution...
  87. pmc Regulatory divergence in Drosophila revealed by mRNA-seq
    C Joel McManus
    Department of Genetics and Developmental Biology, University of Connecticut Stem Cell Institute, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Genome Res 20:816-25. 2010
    ..sequencing, we quantified total and allele-specific mRNA expression levels genome-wide in two closely related Drosophila species (D. melanogaster and D. sechellia) and their F(1) hybrids...
  88. pmc High-fat-diet-induced obesity and heart dysfunction are regulated by the TOR pathway in Drosophila
    Ryan T Birse
    NASCR Center, Sanford Burnham Medical Research Institute, La Jolla, CA 92037, USA
    Cell Metab 12:533-44. 2010
    ..Here, we use Drosophila to test the hypothesis that HFD-induced obesity and associated cardiac complications have early evolutionary ..
  89. ncbi Regulatory changes underlying expression differences within and between Drosophila species
    Patricia J Wittkopp
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Nat Genet 40:346-50. 2008
    ..of cis- and trans-regulatory changes to variable intra- and interspecific gene expression using four strains of Drosophila melanogaster, three strains of D. simulans and a total of 78 genes...
  90. pmc RNA toxicity is a component of ataxin-3 degeneration in Drosophila
    Ling Bo Li
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6018, USA
    Nature 453:1107-11. 2008
    ..Here we provide evidence for a pathogenic role of the CAG repeat RNA in polyQ toxicity using Drosophila. In a Drosophila screen for modifiers of polyQ degeneration induced by the spinocerebellar ataxia type 3 (SCA3) ..
  91. pmc CNTNAP2 and NRXN1 are mutated in autosomal-recessive Pitt-Hopkins-like mental retardation and determine the level of a common synaptic protein in Drosophila
    Christiane Zweier
    Institute of Human Genetics, Friedrich Alexander University Erlangen Nuremberg, 91054 Erlangen, Germany
    Am J Hum Genet 85:655-66. 2009
    ..Using Drosophila as a model, we now show that, as known for fly Nrx-I, the CASPR2 ortholog Nrx-IV might also localize to synapses...
  92. ncbi Pharmacological rescue of synaptic plasticity, courtship behavior, and mushroom body defects in a Drosophila model of fragile X syndrome
    Sean M J McBride
    Section of Molecular Cardiology, Department of Medicine, Medical Scientist Training Program, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Neuron 45:753-64. 2005
    ..A Drosophila model for Fragile X syndrome, based on the loss of dfmr1 activity, exhibits phenotypes that bear similarity to ..
  93. pmc Sepsid even-skipped enhancers are functionally conserved in Drosophila despite lack of sequence conservation
    Emily E Hare
    Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California, United States of America
    PLoS Genet 4:e1000106. 2008
    ..locus from six species of scavenger flies (Sepsidae) that are highly diverged from the model species Drosophila melanogaster, but share its basic patterns of developmental gene expression...
  94. ncbi Mutations of the human homolog of Drosophila patched in the nevoid basal cell carcinoma syndrome
    H Hahn
    Centre for Molecular and Cell Biology, University of Queensland, St Lucia, Australia
    Cell 85:841-51. 1996
    ..A human sequence (PTC) with strong homology to the Drosophila segment polarity gene, patched, was isolated from a YAC and cosmid contig of the NBCCS region...
  95. pmc Wild-type and A315T mutant TDP-43 exert differential neurotoxicity in a Drosophila model of ALS
    Patricia S Estes
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA
    Hum Mol Genet 20:2308-21. 2011
    ..within TDP-43, efforts have been directed towards generating animal models of ALS in mouse, zebrafish, Drosophila and worms...
  96. pmc Evolution in the fast lane: rapidly evolving sex-related genes in Drosophila
    Wilfried Haerty
    Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada
    Genetics 177:1321-35. 2007
    A large portion of the annotated genes in Drosophila melanogaster show sex-biased expression, indicating that sex and reproduction-related genes (SRR genes) represent an appreciable component of the genome...
  97. ncbi Estimating the rate of adaptive molecular evolution in the presence of slightly deleterious mutations and population size change
    Adam Eyre-Walker
    Centre for the Study of Evolution and School of Life Sciences, University of Sussex, Brighton, United Kingdom
    Mol Biol Evol 26:2097-108. 2009
    ..We analyze several data sets of protein-coding genes and noncoding regions from hominids and Drosophila. In Drosophila genes, we estimate that approximately 50% of amino acid substitutions and approximately 20% of ..
  98. pmc Drosophila dosage compensation: a complex voyage to the X chromosome
    Marnie E Gelbart
    Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Development 136:1399-410. 2009
    ..In Drosophila, the male-specific lethal (MSL) ribonucleoprotein complex mediates dosage compensation by upregulating ..
  99. pmc A Drosophila model for TDP-43 proteinopathy
    Yan Li
    Department of Neurology, Lurie Cancer Center, Center for Genetic Medicine, Northwestern University School of Medicine, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 107:3169-74. 2010
    ....
  100. pmc puckered encodes a phosphatase that mediates a feedback loop regulating JNK activity during dorsal closure in Drosophila
    E Martin-Blanco
    Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, UK
    Genes Dev 12:557-70. 1998
    ..Here, we describe a new member of this family encoded by the puckered gene of Drosophila. Mutations in this gene lead to cytoskeletal defects that result in a failure in dorsal closure related to those ..
  101. pmc Genome-wide analysis of the interaction between the endosymbiotic bacterium Wolbachia and its Drosophila host
    Zhiyong Xi
    Department of Entomology University of Kentucky Lexington, KY 40546, USA
    BMC Genomics 9:1. 2008
    ..Despite substantial scientific effort, the molecular mechanisms underlying the Wolbachia/host interaction are unknown...

Research Grants77

  1. Discovery of Small Molecule MBT Domain Antagonists
    STEPHEN VERNON FRYE; Fiscal Year: 2010
    ..This proposal specifically aims to develop potent antagonists of methyl-lysine recognition by human and Drosophila MBT domain containing proteins in order to permit exploration of the biological consequences of blocking this ..
  2. Sphingolipid Metabolism in Drosophila Development
    Julie D Saba; Fiscal Year: 2013
    ..Studies supported by this grant have explored the role of sphingolipids in the model organism Drosophila melanogaster...
  3. Targeted siRNA nanotechnology for intravesical treatment of urologicaldiseases
    Robert M Weiss; Fiscal Year: 2010
    ..and Kim Woodrow of the Yale University Department of Bioengineering, we have shown that antennapedia (AP), a Drosophila transcription factor that facilitates uptake of peptides and oligonucleotides into mammalian cells, when ..
  4. Functional Genomics of the Mitochondrial Proteome
    Dennis R Winge; Fiscal Year: 2011
    ..define the physiological role of these OXPHOS assembly proteins in the context of a multicellular model organism Drosophila. Aim 5 will generate mouse models of mitochondrial dysfunction, harboring tissue-restricted mutations these ..
  5. GENETIC ANALYSIS OF POLARITY
    RUTH M STEWARD; Fiscal Year: 2010
    Abstract: The developmental mechanisms of human and Drosophila blood systems show remarkable parallels...
  6. Using Drosophila to understand WNK and SPAK/OSR1 regulation of SLC12 cotransporte
    AYLIN RACHEL RODAN; Fiscal Year: 2013
    ..D., and fellowship studies, and in particular previous experience studying Drosophila melanogaster, by developing new scientific skills in physiology and biochemistry...
  7. Genetic Studies of Optic Atrophy
    Taosheng Huang; Fiscal Year: 2012
    ..of an effective therapeutic agent for optic atrophies, recently we have generated an Opa1 knockout model in Drosophila. By analyzing phenotypes in the developing and adult Drosophila eyes, we found that the heterozygous mutation of ..
  8. Ceramide Metabolism and Photoreceptor Homeostasis
    Usha R Acharya; Fiscal Year: 2013
    ..findings that enzymes of the sphingolipid biosynthetic pathway and their metabolites are important regulators of Drosophila photoreceptor structure, function, and modulation of this pathway can suppress retinal degeneration in a set of ..
  9. Novel genomic effects of Y-linked polymorphisms
    Daniel L Hartl; Fiscal Year: 2012
    ..The novelty emerges from our recent discovery that the Y chromosome of Drosophila melanogaster is polymorphic for sequences that differentially affect the expression of many hundreds of ..
  10. PTEN TUMOR SUPPRESSOR AND SIGNAL TRANSDUCTION
    RAMON E PARSONS; Fiscal Year: 2012
    ..elegans and drosophila. In these species, PTEN acts downstream of the insulin/IGF receptor, the insulin receptor substrate (IRS) ..
  11. Phosphotyrosine signaling pathways controlling tracheal tube geometry
    KAI G ZINN; Fiscal Year: 2013
    ..The respiratory (tracheal) system of the Drosophila larva has provided a useful genetic model for the study of the development of complex branched tubular networks...
  12. Diverse Roles for RIC8 During G Protein Signaling in Fungi
    Katherine A Borkovich; Fiscal Year: 2012
    ..RIC8 is required for asymmetric cell division in both Caenorhabditis elegans and Drosophila. Loss of ric8 leads to reduced levels of G proteins in Drosophila, suggesting a chaperone function in addition ..
  13. Analysis of the role of the VRK1 protein kinase in mammalian fertility
    Paula Traktman; Fiscal Year: 2011
    ..The VRK1 orthologs in Drosophila and C. elegans are essential proteins whose disruption causes sterility associated with meiotic defects...
  14. BMP signaling in vertebrate development
    Ken W Y Cho; Fiscal Year: 2013
    ..We have recently provided evidence that the evolutionarily conserved orthologs of the Drosophila gene schnurri encode a common partner that may be used more widely to promote BMP target gene recognition...
  15. Persisting Grand Mal and Myoclonic Seizures in JME including Veterans
    Antonio V Delgado-Escueta; Fiscal Year: 2013
    ..The results guide which putative epilepsy genes have priority for studies of functions and disease mechanisms in Drosophila melanogaster (Aim 3), mice (Aim 2) and humans (Aim 1)...
  16. 5500 QTRAP
    John W Turk; Fiscal Year: 2010
    ..Sasanka Ramanadham, WU);(3) Using Drosophila to identify candidate modifiers of glucose toxicity (Dr...
  17. Analysis of the functions of axon guidance genes during vector mosquito developme
    MOLLY DUMAN SCHEEL; Fiscal Year: 2012
    ..In recent years, evolutionary developmental biologists have applied knowledge of developmental genetics in Drosophila melanogaster, a genetically tractable model organism, to better understand development of other arthropods...
  18. Oxidative stress and neurogenetic networks in Drosophila
    Robert R H Anholt; Fiscal Year: 2010
    ..b>Drosophila melanogaster provides a genetically tractable model system, because the genetic background and environmental ..
  19. Regulation of Rhox5 Transcriptional Elongation
    Marcy E Richardson; Fiscal Year: 2013
    ..Recent studies conducted in human embryonic stem cells and Drosophila melanogaster embryos have identified transcriptional elongation as a widely used alternative control mechanism ..
  20. Germ Cell Migration in Drosophila.
    Ruth Lehmann; Fiscal Year: 2012
    ..We have used Drosophila to identify signals produced by the soma that guide migrating germ cells...
  21. Genetic Control of Axial Patterning in Drosophila Eye
    Amit Singh; Fiscal Year: 2010
    ..The fruit fly, Drosophila, provides an ideal model for genetic analysis of dorsoventral (DV) patterning and growth of the eye primordium...
  22. Melanin-Concentrating Hormone: Ancestral Role in Feeding &Sleep Regulation
    Philippe Mourrain; Fiscal Year: 2013
    ..As MCH is not found in non-vertebrate models like Drosophila and C...
  23. Tunable Insulin Resistance in a Drosophila Model of Diabetes
    ELENA M GRACHEVA; Fiscal Year: 2010
    ..platform optimized for high-throughput drug screening in situ by generating human diseases in the fruit fly Drosophila. Our whole-organism approach holds the promise of producing better safety and efficacy data than traditional pre-..
  24. Epigenetic mechanisms of TDP43-mediated neurodegeneration
    Amit Berson; Fiscal Year: 2013
    ..question, we recently performed an in-vivo RNAi screen for epigenetic modifiers of neurodegeneration in Drosophila, a powerful genetically amendable animal model...
  25. TRANSCRIPTIONAL REPRESSION BY POLYCOMB GROUP PRODUCTS
    Jeffrey A Simon; Fiscal Year: 2012
    ..genetic and biochemical methods will be used to study the Polycomb group (PcG) transcriptional repressors of Drosophila, which provide one of the premier models for deciphering chromatin mechanisms in development...
  26. Dissecting the role of one neuronal RhoGEF amongst many: the Kalirin-7 null mouse
    Richard E Mains; Fiscal Year: 2012
    ..Mammals also express Trio, a highly homologous, but non- redundant gene. The single Kalirin/Trio gene in Drosophila and C. elegans plays an essential role within and outside of the nervous system...
  27. AAA ATPases linking mitochondria with microtubule processing in flies and yeast
    Karen G Hales; Fiscal Year: 2012
    ..Many human genetic neurodegenerative disorders are associated with defects of mitochondrial dynamics. Drosophila melanogaster spermatogenesis is an ideal model system for genetic dissection of conserved mechanisms of ..
  28. The role of satellite rasiRNA in the Segregation Distorter system of Drosophila
    AMANDA MARIE LARRACUENTE; Fiscal Year: 2013
    ..If SD affects Rsp rasiRNA production, then SD genotype will correlate with Rsp rasiRNA abundance in postmeiotic testis dissections. ..
  29. Molecular Genetics of Caloric Restriction in Aging Flies
    Blanka Rogina; Fiscal Year: 2013
    ..contributions to our understanding of the effects of age and caloric intake on physiology and longevity of adult Drosophila. Mutation in the Indy gene in the fruit fly, Drosophila melanogaster, dramatically extends life span...
  30. SIRT1 as a regulator of health and lifespan of mammals
    David A Sinclair; Fiscal Year: 2013
    ..Recent evidence from C. elegans and Drosophila indicates that alterations in mitochondrial function in one cell type (e.g...
  31. Is symmetrical dimethylation of Arginine 2 of Histone H3 used as an epigenetic si
    Marjorie A Oettinger; Fiscal Year: 2010
    ..Fourth, we will analyze these modifications in model organisms (S. cerevisiae, S. pombe and Drosophila melanogaster), determining both the genome-wide localization pattern of the modifications and whether they are ..
  32. Humanized Transgenic Mice Reproduce the Disease Evolution in Severe RA
    Grete Sonderstrup; Fiscal Year: 2010
    ..model by introducing the RA-susceptible HLA- DR*0401 gene and a luciferase transgene (controlled by a minimal Drosophila promoter activated by 3 NF:B binding sites), which will facilitate continuous in vivo monitoring of the disease ..
  33. Molecular Physiology of the Epitrachael Endocrine System
    Michael E Adams; Fiscal Year: 2012
    ..to activate or ablate specific peptidergic ensembles to test their necessary roles in the ecdysis sequence in Drosophila, Aedes, and Bombyx...
  34. Ligands and cofactors required for RORgt function in the immune system
    Dan Littman; Fiscal Year: 2013
    ..We have found that RORyt has transcriptional activity in Drosophila S2 cells, and have exploited this observation to perform a genome-wide RNA interference screen...
  35. Analyzing Anthrax Toxins in Drosophilia
    Ethan Bier; Fiscal Year: 2013
    ..resulting in barrier disruption We discovered this novel effect of anthrax toxins using the model genetic system Drosophila melanogaster (fruit fly) and then validated these effects in human vascular endothelial cells as well as in vivo ..
  36. Genome-wide Identification of Immediate Early Genes in Insects
    Anandasankar Ray; Fiscal Year: 2012
    ..A few genes have been identified as IEGs in mammals, but very little is known about IEGs in insects such as Drosophila melanogaster, which is used as a model to study these biological process...
  37. Ecd as a regulator of cell cycle and breast oncogenesis
    Vimla Band; Fiscal Year: 2013
    ..We have identified the mammalian ortholog of Drosophila ecdysoneless (Ecd) protein as a novel and essential regulator of Rb-E2F-dependent cell cycle progression...
  38. Germ Cell Migration in Drosophila.
    Ruth Lehmann; Fiscal Year: 2013
    ..We have used Drosophila to identify signals produced by the soma that guide migrating germ cells...
  39. EGF RECEPTOR MEDIATED SIGNALING IN DROSOPHILA
    GERTRUD M SCHUPBACH; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): This research project investigates the mechanisms that activate the Drosophila Epidermal Growth Factor receptor (Egfr) during oogenesis, and the cellular pathways that mediate the response to this ..
  40. Laser Spinning Disc Confocal System for live-cell and live-organism microscopy
    Martha C Soto; Fiscal Year: 2012
    ..elegans as a model system to study human ciliopathies, or diseases of cilia. Drosophila studies of meiosis in the McKim lab have identified molecules required for proper fertility through the ..
  41. CSHL Eukaryotic DNA Replication Conference
    David J Stewart; Fiscal Year: 2013
    ..including studies of: (1) chromosomal replication and gene amplification in organisms as diverse as yeast, Drosophila, Xenopus and mammalian cells;(2) the replication of viral chromosomes, including SV40, polyomavirus, ..
  42. Expression control in Drosophila splicing assembly factor RNP-4F
    JACK VAUGHN; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Drosophila RNP-4F is an ortholog to human p110, which functions with the pre-mRNA processing (PRP) protein class as a recycling factor to carry U4- and U6-snRNPs to the assembling spliceosome...
  43. CELL CYCLE CONTROL IN EARLY DROSOPHILA DEVELOPMENT
    Terry L Orr-Weaver; Fiscal Year: 2013
    ..b>Drosophila is an ideal model organism in which to identify the key regulatory mechanisms and genes that govern ..
  44. Regulation of TRP channels and visual transduction
    Craig Montell; Fiscal Year: 2013
    The long-term goal of our research is to understand the mechanisms underlying phototransduction in the fruitfly, Drosophila melanogaster...
  45. Insulin, IGF and Insulin Signaling: effects on Anopheles lifespan and immunity
    MICHAEL ALLEN RIEHLE; Fiscal Year: 2012
    ..its impacts on mosquito physiology and vector competence that were conceptualized for Caenorhabditis elegans, Drosophila melanogaster and mammals...
  46. Role of FUS in ALS
    Haining Zhu; Fiscal Year: 2013
    ..The Drosophila model we established showed motor function deficiency when FUS was over-expressed in motor neurons...
  47. Genetic and Molecular Studies of Drosophila Chromatin Remodeling Factors
    John W Tamkun; Fiscal Year: 2013
    ..To address these important issues, our laboratory uses Drosophila melanogaster as a model organism to study the function of chromatin-remodeling and modifying enzymes...
  48. 2011 EUKARYOTIC DNA REPLICATION &GENOME MAINTENANCE
    David J Stewart; Fiscal Year: 2011
    ..including studies of: (1) chromosomal replication and gene amplification in organisms as diverse as yeast, Drosophila, Xenopus and mammalian cells;(2) the replication of viral chromosomes, including SV40, polyomavirus, ..
  49. Using Drosophila as a model to understand TDP-43 function in ALS
    David B Morton; Fiscal Year: 2012
    ..We are using the fruit fly, Drosophila melanogaster, as a model to understand the function of TDP- 43 in neurons and the consequences of aberrant ..
  50. How do Amine Neurons Work?
    Edward A Kravitz; Fiscal Year: 2013
    ..These experimental approaches are the farthest advanced in fruit flies (Drosophila melanogaster) where powerful genetic methods allow identification and manipulation of single neurons concerned ..
  51. Regulation of Neuronal Survival by the Rit GTPase
    DOUGLAS ALLEN ANDRES; Fiscal Year: 2012
    ..conserved group of Ras-related GTPases, including two mammalian proteins (Rit and Rin) and a single Drosophila ortholog (Ric), play critical roles in regulating apoptotic signaling...
  52. Phylogenomics and functional diversification of the Heterochromatin Protein 1 gen
    MIA TAUNA LEVINE; Fiscal Year: 2012
    ..Our recent hand-curated computational approach to HP1 annotation across Drosophila genomes, however, expanded this family from three to 14 genes, most of which evolve rapidly...
  53. Role of Stasimon Dysfunction in Spinal Muscular Atrophy
    Livio Pellizzoni; Fiscal Year: 2013
    ..expression is decreased by SMN deficiency and that contributes to SMN-dependent motor neuron dysfunction in Drosophila and zebrafish models of SMA...
  54. Centrosomin and Centrosomes in Cell Division
    Timothy L Megraw; Fiscal Year: 2012
    ..Here we focus on the Centrosomin family of proteins. A key breakthrough came with mutation studies of Drosophila Centrosomin (CNN), showing that it is required in an early process of mitotic centrosome assembly...
  55. Genetic control of quantitative traits associated with the metabolic syndrome
    Maria De Luca; Fiscal Year: 2013
    ..4) Investigate whether genetic variants in human orthologs of the 10 Drosophila candidate genes identified in the previous aims are associated with phenotypic variation in human obesity and ..
  56. The Role of Alternative Splicing Factor Sfrs10 in Neural Development
    Rahul N Kanadia; Fiscal Year: 2010
    ..Previous studies in Drosophila have shown that Sfrs10 is required during sex determination and others have shown that Sfrs10 regulates the AS ..
  57. Molecular and cellular mechanism of Microcephaly
    Arnold Kriegstein; Fiscal Year: 2013
    ..Centrosome defects in Drosophila do not dramatically perturb mitosis in most somatic cells, but the asymmetric division of larval neuroblasts is ..
  58. De novo establishment of Polycomb-group-mediated repression
    Richard S Jones; Fiscal Year: 2010
    ..Originally identified as negative regulators of Drosophila Hox genes, their functions and molecular/biochemical activities have been highly conserved from insects to ..
  59. Nutrition, Deregulation of Imprinted Genes
    Cathrine Hoyo; Fiscal Year: 2013
    ..associated with aberrant methylation and loss of imprinting of insulin-like growth factor-2 (IGF2) and Delta, drosophila homolog-like-1 (DLK1) in the offspring;(2) To determine whether aberrant methylation and loss of imprinting of ..
  60. Non-coding RNAs in the epigenetics of human centromere formation
    PETER WARBURTON; Fiscal Year: 2009
    ..Remarkably, it has been shown in S. pombe, Drosophila, Arabidopsis and mouse that assembly of centromeric heterochromatin actually requires bidirectional cis ..
  61. Nemo-Like Kinase and the Pathogenesis of Spinal and Bulbar Muscular Atrophy
    TIFFANY WERLYNE TODD; Fiscal Year: 2013
    ..Both established and novel Drosophila models of SBMA will be employed to determine if there is a genetic interaction between NLK and the mutant AR ..
  62. Control of developmental timing and body size in Drosophila
    MICHAEL BRENDAN O'CONNOR; Fiscal Year: 2013
    ..processing is required for PTTH signaling and if PKC, PLC and the IP3 receptor, contribute to PTTH signaling in Drosophila and how their activity is coupled to Torso activation...
  63. PAIRING OF HOMOLOGOUS SEQUENCES IN DROSOPHILA
    KENT GOLIC; Fiscal Year: 2002
    ..The fourth specific aim will aim to adapt for Drosophila the "cassette exchange" method of FLP-mediated DNA integration developed by Schlake and Bode...
  64. DOSAGE COMPENSATION IN DROSOPHILA
    Bruce Baker; Fiscal Year: 1999
    ..clear that the mechanisms of dosage compensation are quite distinct in the three most studied eukaryotic system, Drosophila C. elegans, and man. Drosophila dosage compensates by hyperactivation of the single male X chromosome, C...
  65. SEGMENTATION AND PATTERNING IN DROSOPHILA
    Eric Wieschaus; Fiscal Year: 2000
    ..is to understand how complex patterns of cell differentiation are established in epithelial cells during Drosophila embryonic development...
  66. FUNCTIONAL ANALYSIS OF NOVEL GABA RECEPTOR IN DROSOPHILA
    Richard ffrench Constant; Fiscal Year: 1999
    ..recently identified a novel GABA receptor subunit gene Rdl (Resistance to dieldrin) that was cloned from a Drosophila mutant resistant to PTX and cyclodiene insecticides...
  67. Establishing Drosophila as a Model to Study Hutchinson-Gilford Progeria Syndrome
    Sandra Schulze; Fiscal Year: 2009
    ..The experiments described in this proposal will establish the fruit fly Drosophila as a model to study human Lamin A processing...
  68. Generation of Wunen/LPP3-based therapy for muscular dystrophy
    Hannele Ruohola Baker; Fiscal Year: 2010
    ..reduction of LPP3 that reduces the level of active second messenger, S1P can suppress dystrophic phenotypes in Drosophila melanogaster...
  69. Immune Response of Drosophila Against Spiroplasma
    Mariana Mateos; Fiscal Year: 2009
    ..The fruitfly Drosophila melanogaster has provided an unbiased and powerful tool for examination of innate response against many types of ..
  70. A Drosophila Model for Genetic Studies of Metabolism
    Carl S Thummel; Fiscal Year: 2010
    ..The goal of our research is to use the fruit fly, Drosophila melanogaster, as a simple model system to define the central regulatory pathways that control metabolism and ..
  71. EFFECTS OF AGING ON GERMLINE AND SOMATIC STEM CELLS IN DROSOPHILA MELANOGASTER
    MATTHEW WALLENFANG; Fiscal Year: 2007
    ..The research proposed examines the effect of aging on stem cells in the Drosophila testis, which presents 3 major advantages for this type of study...
  72. GENE EXPRESSION DURING DROSOPHILA MYOGENESIS
    Allen Shearn; Fiscal Year: 1999
    We propose to continue systematic molecular genetic investigation of Drosophila muscle development and pathology, the broader goal being enhanced understanding of myopathies afflicting humans and domestic animals...
  73. MECHANISMS OF DNA CROSSLINK REPAIR IN HIGHER EUKARYOTES
    KENNETH BURTIS; Fiscal Year: 1999
    ..clone the Fanconi anemia A (FA-A) gene of man through an analysis of the functionally homologous mus3O8 gene of Drosophila. This hematological disorder is a candidate for human gene therapy...
  74. Genetic and Biochemical Analyses of Germline Development in Drosophila
    Alexey Arkov; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Genetic and Biochemical Analyses of Germline Development in Drosophila During development germline is originated from the embryonic germ cells...
  75. A Drosophila Model for Williams Syndrome Cognitive Processing
    Ralph J Greenspan; Fiscal Year: 2010
    ..This proposal outlines a plan to develop a behavioral/genetic model in Drosophila melanogaster to study the distinctive cognitive phenotype affecting visuospatial processing found in humans with ..
  76. HCO3-transporters in Drosophila and Mosquitoes
    MICHAEL ROMERO; Fiscal Year: 2003
    ..Recently we have cloned a Na+-driven anion exchangers (NDAE1) from Drosophila and immunolocalized NDAE1 to epithelia (gut, Malpighian tubules and salivary glands) as well as the central and ..
  77. CELLULAR COMMUNICATION IN MORPHOGENESIS
    Mark Krasnow; Fiscal Year: 2003
    This is a proposal to identify and characterize response pathways in Drosophila cells that are triggered by lack of oxygen (hypoxia). During the previous funding period, Dr...