genetically modified organisms

Summary

Summary: Organisms whose GENOME has been changed by a GENETIC ENGINEERING technique.

Top Publications

  1. Paparini A, Romano Spica V. Public health issues related with the consumption of food obtained from genetically modified organisms. Biotechnol Annu Rev. 2004;10:85-122 pubmed
    b>Genetically Modified Organisms (GMOs) are a fact of modern agriculture and a major field of discussion in biotechnology...
  2. Hug K. Genetically modified organisms: do the benefits outweigh the risks?. Medicina (Kaunas). 2008;44:87-99 pubmed
    The objective of this literature review is to analyze the implications of using genetically modified organisms (GMOs) as well as international and European position regarding such organisms.
  3. Manuell A, Beligni M, Elder J, Siefker D, Tran M, Weber A, et al. Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast. Plant Biotechnol J. 2007;5:402-12 pubmed
    ..As Chlamydomonas reinhardtii is an edible alga, production of therapeutic proteins in this organism offers the potential for oral delivery of gut-active proteins, such as M-SAA...
  4. Elenis D, Kalogianni D, Glynou K, Ioannou P, Christopoulos T. Advances in molecular techniques for the detection and quantification of genetically modified organisms. Anal Bioanal Chem. 2008;392:347-54 pubmed publisher
    Progress in genetic engineering has led to the introduction of genetically modified organisms (GMOs) whose genomes have been altered by the integration of a novel sequence conferring a new trait...
  5. Cankar K, Chauvensy Ancel V, Fortabat M, Gruden K, Kobilinsky A, Zel J, et al. Detection of nonauthorized genetically modified organisms using differential quantitative polymerase chain reaction: application to 35S in maize. Anal Biochem. 2008;376:189-99 pubmed publisher
    Detection of nonauthorized genetically modified organisms (GMOs) has always presented an analytical challenge because the complete sequence data needed to detect them are generally unavailable although sequence similarity to known GMOs ..
  6. Gurvitz A, Hiltunen J, Kastaniotis A. Identification of a novel mycobacterial 3-hydroxyacyl-thioester dehydratase, HtdZ (Rv0130), by functional complementation in yeast. J Bacteriol. 2008;190:4088-90 pubmed publisher
    ..Mutant cells expressing HtdZ contained dehydratase activity, recovered their respiratory ability, and partially restored de novo lipoic acid synthesis...
  7. Holst Jensen A. Testing for genetically modified organisms (GMOs): Past, present and future perspectives. Biotechnol Adv. 2009;27:1071-82 pubmed publisher
    ..To better understand the suitability and limitations of detection methodologies the evolution of transformation processes for creation of GMOs is briefly reviewed...
  8. Herdewijn P, Marliere P. Toward safe genetically modified organisms through the chemical diversification of nucleic acids. Chem Biodivers. 2009;6:791-808 pubmed publisher
  9. Guo L, Yang H, Qiu B, Xiao X, Xue L, Kim D, et al. Capillary electrophoresis with electrochemiluminescent detection for highly sensitive assay of genetically modified organisms. Anal Chem. 2009;81:9578-84 pubmed publisher
    ..Results showed that, under the optimal conditions, the proposed method can accurately identifying RRS. The corresponding limit of detection (LOD) was below 0.01% with 35 PCR cycles...

Scientific Experts

More Information

Publications62

  1. Shinohara M, Oh S, Hunter N, Shinohara A. Crossover assurance and crossover interference are distinctly regulated by the ZMM proteins during yeast meiosis. Nat Genet. 2008;40:299-309 pubmed publisher
    ..In contrast, crossover assurance requires all ZMM proteins and full-length synaptonemal complexes...
  2. Bonawitz N, Chatenay Lapointe M, Pan Y, Shadel G. Reduced TOR signaling extends chronological life span via increased respiration and upregulation of mitochondrial gene expression. Cell Metab. 2007;5:265-77 pubmed
  3. Michelini E, Simoni P, Cevenini L, Mezzanotte L, Roda A. New trends in bioanalytical tools for the detection of genetically modified organisms: an update. Anal Bioanal Chem. 2008;392:355-67 pubmed publisher
    Despite the controversies surrounding genetically modified organisms (GMOs), the production of GM crops is increasing, especially in developing countries...
  4. Tucker J, Zilinskas R. The promise and perils of synthetic biology. New Atlantis. 2006;12:25-45 pubmed
  5. Torgersen H. The real and perceived risks of genetically modified organisms. EMBO Rep. 2004;5 Spec No:S17-21 pubmed
  6. Nesvold H, Kristoffersen A, Holst Jensen A, Berdal K. Design of a DNA chip for detection of unknown genetically modified organisms (GMOs). Bioinformatics. 2005;21:1917-26 pubmed
    Unknown genetically modified organisms (GMOs) have not undergone a risk evaluation, and hence might pose a danger to health and environment. There are, today, no methods for detecting unknown GMOs...
  7. Braat H, Rottiers P, Hommes D, Huyghebaert N, Remaut E, Remon J, et al. A phase I trial with transgenic bacteria expressing interleukin-10 in Crohn's disease. Clin Gastroenterol Hepatol. 2006;4:754-9 pubmed
    ..However, treatment of patients with a living, genetically modified bacterium raises questions about the safety for human subjects per se and the biologic containment of the transgene...
  8. Miraglia M, Berdal K, Brera C, Corbisier P, Holst Jensen A, Kok E, et al. Detection and traceability of genetically modified organisms in the food production chain. Food Chem Toxicol. 2004;42:1157-80 pubmed
    Both labelling and traceability of genetically modified organisms are current issues that are considered in trade and regulation...
  9. Taverniers I, Van Bockstaele E, De Loose M. Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs). Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet. 2001;66:469-72 pubmed
    For the quantification of genetically modified organisms (GMOs) in foods and feeds, real-time PCR is currently the most widely applied technique...
  10. Cases I, De Lorenzo V. Genetically modified organisms for the environment: stories of success and failure and what we have learned from them. Int Microbiol. 2005;8:213-22 pubmed
    ..In this review, several valuable spin-offs of past research into genetically modified organisms with environmental applications are discussed, along with the impact of Systems Biology and Synthetic ..
  11. Hill R. Conceptualizing risk assessment methodology for genetically modified organisms. Environ Biosafety Res. 2005;4:67-70 pubmed
  12. Harms H, Wells M, van der Meer J. Whole-cell living biosensors--are they ready for environmental application?. Appl Microbiol Biotechnol. 2006;70:273-80 pubmed
    ..This includes the identification of some popular misconceptions about the qualities and shortcomings of bioreporters...
  13. Xu J, Miao H, Wu H, Huang W, Tang R, Qiu M, et al. Screening genetically modified organisms using multiplex-PCR coupled with oligonucleotide microarray. Biosens Bioelectron. 2006;22:71-7 pubmed
    ..It can detect more than 95% of currently commercial GMO plants and the limits of detection are 0.5% for soybean and 1% for maize. This method is proved to be a new method for routine analysis of GMOs...
  14. Olsen D, Yang C, Bodo M, Chang R, Leigh S, Baez J, et al. Recombinant collagen and gelatin for drug delivery. Adv Drug Deliv Rev. 2003;55:1547-67 pubmed
  15. Spök A, Gaugitsch H, Laffer S, Pauli G, Saito H, Sampson H, et al. Suggestions for the assessment of the allergenic potential of genetically modified organisms. Int Arch Allergy Immunol. 2005;137:167-80 pubmed
    ..g., food). Although there is almost no scientific evidence that genetically modified organisms (GMOs) exhibit increased allergenicity compared with the corresponding wild type significant concerns ..
  16. Chan L, Kosuri S, Endy D. Refactoring bacteriophage T7. Mol Syst Biol. 2005;1:2005.0018 pubmed
    ..The viability of our initial design suggests that the genomes encoding natural biological systems can be systematically redesigned and built anew in service of scientific understanding or human intention...
  17. Takahashi Ando N, Ohsato S, Shibata T, Hamamoto H, Yamaguchi I, Kimura M. Metabolism of zearalenone by genetically modified organisms expressing the detoxification gene from Clonostachys rosea. Appl Environ Microbiol. 2004;70:3239-45 pubmed
    ..We therefore used egfp::zhd101 to visually monitor the lactonohydrolase activity in genetically modified organisms and evaluated the usefulness of zhd101 for in vivo detoxification of ZEN. While recombinant E...
  18. Ivask A, François M, Kahru A, Dubourguier H, Virta M, Douay F. Recombinant luminescent bacterial sensors for the measurement of bioavailability of cadmium and lead in soils polluted by metal smelters. Chemosphere. 2004;55:147-56 pubmed
    ..e., in the contact assay). The bioavailability of metals in different soils varied (depending probably on soil type) ranging from 0.5% to 56% for cadmium and from 0.2% to 8.6% for lead...
  19. Han S, Kim D. Inference of protein complex activities from chemical-genetic profile and its applications: predicting drug-target pathways. PLoS Comput Biol. 2008;4:e1000162 pubmed publisher
    ..Such efforts will contribute to predicting more precisely relevant pathways including target proteins that interact directly with bioactive compounds...
  20. Košir A, Arulandhu A, Voorhuijzen M, Xiao H, Hagelaar R, Staats M, et al. ALF: a strategy for identification of unauthorized GMOs in complex mixtures by a GW-NGS method and dedicated bioinformatics analysis. Sci Rep. 2017;7:14155 pubmed publisher
    The majority of feed products in industrialised countries contains materials derived from genetically modified organisms (GMOs). In parallel, the number of reports of unauthorised GMOs (UGMOs) is gradually increasing...
  21. Rupper A, Cardelli J. Induction of guanylate binding protein 5 by gamma interferon increases susceptibility to Salmonella enterica serovar Typhimurium-induced pyroptosis in RAW 264.7 cells. Infect Immun. 2008;76:2304-15 pubmed publisher
    ..The regulation of pyroptosis by the IFN-gamma-induced protein GBP-5 may play an important role in the host defense against Salmonella enterica serovar Typhimurium and perhaps other invasive bacterial pathogens...
  22. Wanat J, Singh N, Alani E. The effect of genetic background on the function of Saccharomyces cerevisiae mlh1 alleles that correspond to HNPCC missense mutations. Hum Mol Genet. 2007;16:445-52 pubmed
    ..These findings suggest that genetic background can play an important role in determining the pathogenicity of MMR alleles and may explain cases of atypical colorectal cancer inheritance...
  23. Li I, Pham E, Truong K. Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics. Biotechnol Lett. 2006;28:1971-82 pubmed
    ..Together these advances in the development of FRET biosensors are beginning to unravel the interconnected and intricate signalling processes as they are occurring in living cells and organisms...
  24. Sotgiu A, Tala M, Sardu G, Coroneo V, Dessi S, Contu P. [Genetically modified organisms: European and Italian legislation to protect citizens' health]. Ig Sanita Pubbl. 2005;61:475-96 pubmed
    ..Personal ethical beliefs regarding food and in particular, the consumption of foods derived from biotechnology should be taken into account when deciding healthcare policy...
  25. Wiehe J, Niesler C, Torzewski J, Zimmermann O, Wiesneth M, Schmitt M, et al. Efficient transient genetic labeling of human CD34+ progenitor cells for in vivo application. Regen Med. 2006;1:223-34 pubmed
    ..Mutz2 cells may serve as an in vitro model for human myeloid HPC. The method described herein has been adopted to Good Manufacturing Practices (GMP) guidelines and is ready for in vivo application...
  26. Jigami Y. Yeast glycobiology and its application. Biosci Biotechnol Biochem. 2008;72:637-48 pubmed
    ..cerevisiae are described in relation to lipid microdomains (also called "lipid rafts"), focusing on the latest findings related to lipid remodeling of GPI-anchored proteins...
  27. Kong Q, Gu J, Cao L, Zhang A, Chen X, Zhao X. Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae. Biotechnol Lett. 2006;28:2033-8 pubmed
    ..Furthermore, acetate and pyruvic acid formation was also reduced in order for cells to maintain redox balance...
  28. Valdez M, Rodríguez I, Sittenfeld A. [Perception about biotechnology in university students in Costa Rica]. Rev Biol Trop. 2004;52:745-56 pubmed
    A survey was carried out to determine the perception and knowledge about biotechnology and genetically modified organisms (GMOs) in a sample (n=750) of university students from three public universities in Costa Rica: Universidad de Costa ..
  29. Del Vescovo V, Casagrande V, Bianchi M, Piccinni E, Frontali L, Militti C, et al. Role of Hog1 and Yaf9 in the transcriptional response of Saccharomyces cerevisiae to cesium chloride. Physiol Genomics. 2008;33:110-20 pubmed publisher
    ..We conclude that the observed early transcriptional modulation of cell wall genes has a crucial role in S. cerevisiae adaptation to cesium...
  30. Zhang J, Yan J, Zheng D, Sun Y, Yuan Z. Expression of mel gene improves the UV resistance of Bacillus thuringiensis. J Appl Microbiol. 2008;105:151-7 pubmed publisher
  31. Jiang J, Li R, Guo X, Chen K, Li S. [Risk assessment for environmental release of genetically engineered microorganism m-CDS-1]. Wei Sheng Wu Xue Bao. 2008;48:1479-85 pubmed
    ..To assess the environmental release risk of genetically engineered microorganism Sphingomonas sp. m-CDS-1...
  32. Zhou S, Wang X. [Expression purification and verification of HBscFv-IFNgamma in Pichia pastoris x33]. Sheng Wu Gong Cheng Xue Bao. 2008;24:423-9 pubmed
    ..9% HBV surface antigen (HBsAg) in the serum of HBV transgenic mice, which shows the antibody of HBscFv-IFNgamma has bioactivity in vivo. Therefore HBscFv-IFNgamma can shed light on the development of a new promising HBV-targeted drug...
  33. Deng X, Hu Z, Yi X. Continuous treatment process of mercury removal from aqueous solution by growing recombinant E. coli cells and modeling study. J Hazard Mater. 2008;153:487-92 pubmed
    ..A kinetic model based on mass balance of Hg(2+) was proposed to simulate the continuous process. The modeling results were in good agreement with the experimental data...
  34. Tammana T, Sahasrabuddhe A, Bajpai V, Gupta C. ADF/cofilin-driven actin dynamics in early events of Leishmania cell division. J Cell Sci. 2010;123:1894-901 pubmed publisher
    ..All these abnormalities are, however, reversed by episomal complementation. Together, these results indicate that actin dynamics regulates early events in Leishmania cell division...
  35. Hardison S, Ravi S, Wozniak K, Young M, Olszewski M, Wormley F. Pulmonary infection with an interferon-gamma-producing Cryptococcus neoformans strain results in classical macrophage activation and protection. Am J Pathol. 2010;176:774-85 pubmed publisher
    ..These studies indicate that phenotype, as opposed to quantity, of infiltrating macrophages correlates with protection against pulmonary C. neoformans infection...
  36. Pei Y, Tarun A, Vaughan A, Herman R, Soliman J, Erickson Wayman A, et al. Plasmodium pyruvate dehydrogenase activity is only essential for the parasite's progression from liver infection to blood infection. Mol Microbiol. 2010;75:957-71 pubmed publisher
    ..This phenotype is similar to that observed for deletions of genes involved in FAS II elongation. The data strongly support the hypothesis that the sole role of PDH is to provide acetyl-CoA for FAS II...
  37. MacDonald R, Debiais Thibaud M, Talbot J, Ekker M. The relationship between dlx and gad1 expression indicates highly conserved genetic pathways in the zebrafish forebrain. Dev Dyn. 2010;239:2298-306 pubmed publisher
    ..Our results indicate the dlx genes are regulated by an evolutionarily conserved genetic pathway and may play a role in GABAergic interneuron differentiation in the zebrafish forebrain...
  38. Quinn R, Mahoney S, Wilkinson B, Thornton D, Stirling C. A novel role for Gtb1p in glucose trimming of N-linked glycans. Glycobiology. 2009;19:1408-16 pubmed publisher
    ..Our data demonstrate that both the MRH and G2B domains of Gtb1p contribute to the Glc2 trimming event but that the MRH domain is essential for Glc1 trimming...
  39. Bossin H, Furlong R, Gillett J, Bergoin M, Shirk P. Somatic transformation efficiencies and expression patterns using the JcDNV and piggyBac transposon gene vectors in insects. Insect Mol Biol. 2007;16:37-47 pubmed
    ..Somatic transformation required the presence of the viral inverted terminal repeat in cis only and did not depend upon non-structural viral proteins...
  40. Chernyakov I, Whipple J, Kotelawala L, Grayhack E, Phizicky E. Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1. Genes Dev. 2008;22:1369-80 pubmed publisher
    ..The RTD pathway is the first to be implicated in the turnover of mature RNA species from the class of stable RNAs. These results and the results of others demonstrate that tRNA, like mRNA, is subject to multiple quality control steps...
  41. Moir D, Ming Di -, Opperman T, Schweizer H, Bowlin T. A high-throughput, homogeneous, bioluminescent assay for Pseudomonas aeruginosa gyrase inhibitors and other DNA-damaging agents. J Biomol Screen. 2007;12:855-64 pubmed
    ..This convenient, inexpensive screen will be useful for rapidly identifying DNA gyrase inhibitors and other DNA-damaging agents, which may lead to potent new antibacterials...
  42. Uehara T, Suzuki K, Yamanaka H, Kizaki T, Sakurai T, Ishibashi Y, et al. SHP-2 positively regulates adipogenic differentiation in 3T3-L1 cells. Int J Mol Med. 2007;19:895-900 pubmed
    ..These results highlight a novel role of SHP-2 in the signal transduction pathways regulating adipocyte differentiation...
  43. Delhaize E, Gruber B, Pittman J, White R, Leung H, Miao Y, et al. A role for the AtMTP11 gene of Arabidopsis in manganese transport and tolerance. Plant J. 2007;51:198-210 pubmed
    ..These findings provide evidence for Mn(2+)-specific transport activity by AtMTP11, and implicate the pre-vacuolar compartments in both Mn(2+) tolerance and Mn(2+) homeostasis mechanisms of Arabidopsis...
  44. Zhu J, Zhang B, Smith E, Drees B, Brem R, Kruglyak L, et al. Integrating large-scale functional genomic data to dissect the complexity of yeast regulatory networks. Nat Genet. 2008;40:854-61 pubmed publisher
    ..We then prospectively validate predictions, providing direct experimental evidence that predictive networks can be constructed by integrating multiple, appropriate data types...
  45. Liem F, Lehr M. Future issues including broadening the scope of the GLP principles. Ann Ist Super Sanita. 2008;44:335-40 pubmed
    ..The GLP inspector will need to have a good understanding of the science supporting the study conduct and the electronic systems that generate process and maintain study records...
  46. Touze J, Bricaire F, Garin D. [Healthcare response to bioterrorism]. Bull Acad Natl Med. 2007;191:993-1003 pubmed
    ..Clinical and research skills should be merged in infection-control centers, bringing a new dynamic to microbiology...
  47. Zhou Q, Jiang X, Zhang H, Du A. [Act-1 core promoter region introduces high-performance transcription of EGFP gene expressed in Caenorhabditis elegans]. Sheng Wu Gong Cheng Xue Bao. 2009;25:336-40 pubmed
    ..elegans, which was closely related to the expression level of EGFP. These results lay the foundation for the further research on gene function of parasitic nematodes using C. elegans...
  48. Seternes T, Tonheim T, Løvoll M, Bøgwald J, Dalmo R. Specific endocytosis and degradation of naked DNA in the endocardial cells of cod (Gadus morhua L.). J Exp Biol. 2007;210:2091-103 pubmed
    ..Despite the low amount of radioactivity found in the head kidney after i.v. injection of 125I-labelled pDNA, the head kidney leukocytes seem to have a high capacity for uptake of 125I-labelled pDNA in vitro...
  49. Johnson R. Commentary: Emerging technologies oversight: research, regulation, and commercialization. J Law Med Ethics. 2009;37:587-93 pubmed publisher
    ..Combining the key attributes with the idea of three phases can help attune oversight to society's needs...
  50. Siripornadulsil S, Dabrowski K, Sayre R. Microalgal vaccines. Adv Exp Med Biol. 2007;616:122-8 pubmed
    ..The effective oral delivery of antigens by microalgae provides a safe and inexpensive mechanism to immunize animals. The applications of microalgal vaccines are currently being investigated...
  51. Simkhada D, Kim E, Lee H, Sohng J. Metabolic engineering of Escherichia coli for the biological synthesis of 7-O-xylosyl naringenin. Mol Cells. 2009;28:397-401 pubmed publisher
    ..Product was verified by HPLCLC/MS and ESI-MS/MS analyses. The reconstructed host can be applied for the production of various classes of glycosylated flavonoids...
  52. Miller H. Biotech's defining moments. Trends Biotechnol. 2007;25:56-9 pubmed
    ..Greater precision in terminology would improve the lot of scientists, the quality of public policy and, eventually, human and environmental health...
  53. Katz M, Evans C, Heagney E, vanKuyk P, Kelly J, Cheetham B. Mutations in genes encoding sorting nexins alter production of intracellular and extracellular proteases in Aspergillus nidulans. Genetics. 2009;181:1239-47 pubmed publisher
    ..Analysis of serine protease gene expression suggests that an XprG-independent mechanism for regulation of extracellular protease gene expression in response to carbon starvation exists and is activated in the pseudorevertants...