ethyldimethylaminopropyl carbodiimide


Summary: Carbodiimide cross-linking reagent.

Top Publications

  1. Lee M, Hung C, Cheng J, Wang Y. A new anti-adhesion film synthesized from polygalacturonic acid with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide crosslinker. Biomaterials. 2005;26:3793-9 pubmed
    ..The newly developed PGA-EDC film thus has a great potential for future use in clinical applications. ..
  2. Lu X, Song C, Szulwach K, Wang Z, Weidenbacher P, Jin P, et al. Chemical modification-assisted bisulfite sequencing (CAB-Seq) for 5-carboxylcytosine detection in DNA. J Am Chem Soc. 2013;135:9315-7 pubmed publisher
    ..We found that the modified 5caC can survive the bisulfite treatment without deamination. Therefore, this chemical labeling coupled with bisulfite treatment provides a base-resolution detection and sequencing method for 5caC. ..
  3. Makrides V, Shen T, Bhatia R, Smith B, Thimm J, Lal R, et al. Microtubule-dependent oligomerization of tau. Implications for physiological tau function and tauopathies. J Biol Chem. 2003;278:33298-304 pubmed
  4. Markowicz M, Heitland A, Steffens G, Pallua N. Effects of modified collagen matrices on human umbilical vein endothelial cells. Int J Artif Organs. 2005;28:1251-8 pubmed
    ..Polymerized collagen scaffolds presented biointeractive systems with integrated angiogenic activity. This may become a useful tool in the clinical therapy of disorders connected with wound healing...
  5. Andreev O, Reshetnyak Y. Mechanism of formation of actomyosin interface. J Mol Biol. 2007;365:551-4 pubmed
    ..The sequential mechanism of formation of actomyosin interface starting from one end and developing towards the barbed end might be involved in force generation and directional movement in actin-myosin system. ..
  6. Chang J, Lin J, Yao C, Chen J, Lai T, Chen Y. In vivo evaluation of a biodegradable EDC/NHS-cross-linked gelatin peripheral nerve guide conduit material. Macromol Biosci. 2007;7:500-7 pubmed
    ..These results indicate the superiority of the ENG materials and suggest that the novel ENG conduits provide a promising tool for neuro-regeneration. ..
  7. Seno K, Matumura K, Oshita K, Oshima M, Motomizu S. Determination of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow-injection analysis based on a specific condensation reaction between malonic acid and ethylenediamine. Anal Sci. 2009;25:389-93 pubmed
    ..The limit of detection (LOD) and the relative standard deviation (RSD) were 2 x 10(-6) M and 1.0%, respectively. This reaction is proceeded in aqueous solution and specific for EDC.HCl. ..
  8. Kim H, Lee J, Kim T, Lee E, Oh K, Lee D, et al. Albumin-coated porous hollow poly(lactic-co-glycolic acid) microparticles bound with palmityl-acylated exendin-4 as a long-acting inhalation delivery system for the treatment of diabetes. Pharm Res. 2011;28:2008-19 pubmed publisher
    ..To study the development of porous poly(lactic-co-glycolic acid) microparticles (PLGA MPs) coated initially with albumin and then with palmityl-acylated exendin-4 (Pal-Ex4) as an inhalation system for treating diabetes...
  9. Furukawa Y, Matsuda F, Ishimori K, Morishima I. Investigation of the electron-transfer mechanism by cross-linking between Zn-substituted myoglobin and cytochrome b(5). J Am Chem Soc. 2002;124:4008-19 pubmed

More Information

Publications108 found, 100 shown here

  1. Liu Y, Gan L, Carlsson D, Fagerholm P, Lagali N, Watsky M, et al. A simple, cross-linked collagen tissue substitute for corneal implantation. Invest Ophthalmol Vis Sci. 2006;47:1869-75 pubmed
  2. Sam S, Touahir L, Salvador Andresa J, Allongue P, Chazalviel J, Gouget Laemmel A, et al. Semiquantitative study of the EDC/NHS activation of acid terminal groups at modified porous silicon surfaces. Langmuir. 2010;26:809-14 pubmed publisher
    ..Results are accounted for by NHS surface adsorption and a kinetic competition between the various EDC-induced surface reactions. ..
  3. Bedran Russo A, Vidal C, dos Santos P, Castellan C. Long-term effect of carbodiimide on dentin matrix and resin-dentin bonds. J Biomed Mater Res B Appl Biomater. 2010;94:250-5 pubmed publisher
    ..05). Biomimetic use of EDC/NHS to induce exogenous collagen cross-links resulted in increased mechanical properties and stability of dentin matrix and dentin-resin interfaces. ..
  4. Wang X, Du Y, Li Y, Li D, Sun R. Fluorescent identification and detection of Staphylococcus aureus with carboxymethyl chitosan/CdS quantum dots bioconjugates. J Biomater Sci Polym Ed. 2011;22:1881-93 pubmed publisher
    ..Considering the simplicity and cost-efficiency of this method, its application in the identification and quantification of bacteria in clinical, food and environmental samples is anticipated. ..
  5. Lee K, Tey B, Ho K, Tejo B, Tan W. Nanoglue: an alternative way to display cell-internalizing peptide at the spikes of hepatitis B virus core nanoparticles for cell-targeting delivery. Mol Pharm. 2012;9:2415-23 pubmed publisher
    ..This study demonstrated a proof of principle for cell-targeting delivery via "nanoglue" bioconjugation. ..
  6. McLachlin D, Dunn S. Disulfide linkage of the b and delta subunits does not affect the function of the Escherichia coli ATP synthase. Biochemistry. 2000;39:3486-90 pubmed
    ..These results are consistent with a permanent association of b(2) with delta as suggested by the rotational model of enzyme function. ..
  7. Kalkhof S, Ihling C, Mechtler K, Sinz A. Chemical cross-linking and high-performance Fourier transform ion cyclotron resonance mass spectrometry for protein interaction analysis: application to a calmodulin/target peptide complex. Anal Chem. 2005;77:495-503 pubmed
    ..As such it is applicable as a general strategy for the investigation of the spatial organization of protein complexes and complements existing techniques, such as X-ray crystallography and NMR spectroscopy. ..
  8. Novak P, Kruppa G. Intra-molecular cross-linking of acidic residues for protein structure studies. Eur J Mass Spectrom (Chichester). 2008;14:355-65 pubmed publisher
  9. Jose M, Thomas V, Xu Y, Bellis S, Nyairo E, Dean D. Aligned bioactive multi-component nanofibrous nanocomposite scaffolds for bone tissue engineering. Macromol Biosci. 2010;10:433-44 pubmed publisher
    ..Crosslinking with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride resulted in improved mechanical properties of the scaffolds and improved degradation stability, under physiological conditions. ..
  10. Usha R, Sreeram K, Rajaram A. Stabilization of collagen with EDC/NHS in the presence of L-lysine: a comprehensive study. Colloids Surf B Biointerfaces. 2012;90:83-90 pubmed publisher
    ..From the present study, it is possible to conclude that the pre-treatment of collagen with L-lysine enhances EDC/NHS cross linking and can be used for biomaterial applications. ..
  11. Karabekmez F, Zhao C. Surface treatment of flexor tendon autograft and allograft decreases adhesion without an effect of graft cellularity: a pilot study. Clin Orthop Relat Res. 2012;470:2522-7 pubmed publisher
    ..We therefore presume this surface modification would not adversely affect graft healing. ..
  12. Zhao C, Gao Q, Roberts A, Shaffer S, Doneanu C, Xue S, et al. Cross-linking mass spectrometry and mutagenesis confirm the functional importance of surface interactions between CYP3A4 and holo/apo cytochrome b(5). Biochemistry. 2012;51:9488-500 pubmed publisher
    ..The results indicate that apo b(5) can dock with CYP3A4 in a manner analogous to that of holo b(5), so electron transfer from cyt b(5) is not required for its effects. ..
  13. Vermeeren V, Wenmackers S, Daenen M, Haenen K, Williams O, Ameloot M, et al. Topographical and functional characterization of the ssDNA probe layer generated through EDC-mediated covalent attachment to nanocrystalline diamond using fluorescence microscopy. Langmuir. 2008;24:9125-34 pubmed publisher
    ..However, purely COOH-modified NCD samples, patterned with H-terminated regions and treated with a controlled amount of probe DNA, proved the most efficient in fulfilling these tasks. ..
  14. Liu C, Yu S. Cationic nanoemulsions as non-viral vectors for plasmid DNA delivery. Colloids Surf B Biointerfaces. 2010;79:509-15 pubmed publisher
    ..A facile and inexpensive in situ modification for producing cationic nanoemulsions was developed. The results show the potential of amino acid-modified cationic nanoemulsions as non-viral vectors for gene delivery. ..
  15. Grasman J, Page R, Dominko T, Pins G. Crosslinking strategies facilitate tunable structural properties of fibrin microthreads. Acta Biomater. 2012;8:4020-30 pubmed publisher
    ..Ultimately, we anticipate that we can strategically exploit these properties to promote site-specific tissue regeneration. ..
  16. Mazzoni A, Apolonio F, Saboia V, Santi S, Angeloni V, Checchi V, et al. Carbodiimide inactivation of MMPs and effect on dentin bonding. J Dent Res. 2014;93:263-8 pubmed publisher
    ..The correlative analysis used in this study demonstrated that EDC could contribute to inactivate endogenous dentin MMPs within the hybrid layer created by etch-and-rinse adhesives. ..
  17. Nadler S, Dischino D, Malacko A, Cleaveland J, Fujihara S, Marquardt H. Identification of a binding site on Hsc70 for the immunosuppressant 15-deoxyspergualin. Biochem Biophys Res Commun. 1998;253:176-80 pubmed
    ..These results suggest that in addition to the ATP binding domain, there are two additional substrate binding domains on Hsc70. DSG should provide a tool for understanding the role of the EEVD motif in biological processes. ..
  18. Pliszka B, Redowicz M, Stepkowski D. Interaction of the N-terminal part of the A1 essential light chain with the myosin heavy chain. Biochem Biophys Res Commun. 2001;281:924-8 pubmed
    ..Localization of residues which can possibly react with the cross-linker suggests that the interaction might involve the N-terminal residues of the A1 light chain and the converter region of the heavy chain. ..
  19. Young J, Cheng K, Tsou T, Liu H, Wang H. Preparation of cross-linked hyaluronic acid film using 2-chloro-1-methylpyridinium iodide or water-soluble 1-ethyl-(3,3-dimethylaminopropyl)carbodiimide. J Biomater Sci Polym Ed. 2004;15:767-80 pubmed
    ..The obtained results from elemental analyses, FT-IR spectra and NMR spectra also indicate that acylurea groups were introduced into EDC-cross-linked HA films. ..
  20. DiBella L, Gorbatyuk O, Sakato M, Wakabayashi K, Patel King R, Pazour G, et al. Differential light chain assembly influences outer arm dynein motor function. Mol Biol Cell. 2005;16:5661-74 pubmed
    ..These data suggest that LC2, LC6, and LC9 have different roles in outer arm assembly and are required for wild-type motor function in the Chlamydomonas flagellum. ..
  21. Moshnikova A, Afanasyev V, Proussakova O, Chernyshov S, Gogvadze V, Beletsky I. Cytotoxic activity of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide is underlain by DNA interchain cross-linking. Cell Mol Life Sci. 2006;63:229-34 pubmed
    ..Thus, both in vitro and in vivo, EDC exhibits cytotoxic activity, which may be of potential therapeutic use. ..
  22. Yadav S, Devi R, Kumari S, Yadav S, Pundir C. An amperometric oxalate biosensor based on sorghum oxalate oxidase bound carboxylated multiwalled carbon nanotubes-polyaniline composite film. J Biotechnol. 2011;151:212-7 pubmed publisher
    ..93 and rapid response within 5s at a potential of 0.4V vs Ag/AgCl. The sensitivity was approximately 0.0113 ?A/?M with a detection limit of 3.0 ?M. Proposed oxalate biosensor was successfully applied to human urine sample. ..
  23. Iwamoto H, Oiwa K, Suzuki T, Fujisawa T. X-ray diffraction evidence for the lack of stereospecific protein interactions in highly activated actomyosin complex. J Mol Biol. 2001;305:863-74 pubmed
    ..The results suggest that the stereospecific binding sites, which have been considered important for actomyosin cycling, are paradoxically left unoccupied for most of the time in this highly activated actomyosin complex. ..
  24. Park S, Park J, Kim H, Song M, Suh H. Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking. Biomaterials. 2002;23:1205-12 pubmed
    ..625% glutaraldehyde treated membranes. In cytotoxicity test using L929 fibroblastic cells, the EDC-cross-linked membranes demonstrated no significant toxicity. ..
  25. Richert L, Boulmedais F, Lavalle P, Mutterer J, Ferreux E, Decher G, et al. Improvement of stability and cell adhesion properties of polyelectrolyte multilayer films by chemical cross-linking. Biomacromolecules. 2004;5:284-94 pubmed
    ..Whereas the non cross-linked films are highly resistant to cell adhesion, the cells adhere and spread well on the cross-linked films. ..
  26. Schafer I, Rössle M, Biukovic G, Muller V, Gruber G. Structural and functional analysis of the coupling subunit F in solution and topological arrangement of the stalk domains of the methanogenic A1AO ATP synthase. J Bioenerg Biomembr. 2006;38:83-92 pubmed
    ..Based on these data the surface of contact of B-F could be mapped in the high-resolution structure of subunit B of the A(1)A(O) ATP synthase. ..
  27. Gong Z, Xiong H, Long X, Wei L, Li J, Wu Y, et al. Use of synovium-derived stromal cells and chitosan/collagen type I scaffolds for cartilage tissue engineering. Biomed Mater. 2010;5:055005 pubmed publisher
    ..It suggested that SDSCs coupled with CS/COL-I scaffolds treated sequentially with TGF-?1 and bFGF in vitro were highly competent for engineered cartilage formation in vitro and in vivo. ..
  28. Menzel C, Silbernagl J, Laffleur F, Leichner C, Jelkmann M, Huck C, et al. 2,2'Dithiodinicotinyl ligands: Key to more reactive thiomers. Int J Pharm. 2016;503:199-206 pubmed publisher
    ..02). Accordingly, the double preactivation seems to be a promising strategy in order to obtain entirely preactivated polymers with enhanced mucoadhesive properties. ..
  29. Nosaka K. High affinity of acid phosphatase encoded by PHO3 gene in Saccharomyces cerevisiae for thiamin phosphates. Biochim Biophys Acta. 1990;1037:147-54 pubmed
    ..These results also indicate that the high affinity of the thiamin-repressible acid phosphatase for thiamin phosphates is due to the thiamin-binding properties of this enzyme. ..
  30. Van Dijk J, Knight A, Molloy J, Chaussepied P. Characterization of three regulatory states of the striated muscle thin filament. J Mol Biol. 2002;323:475-89 pubmed
    ..These data provide new insights concerning the difference in cooperativity of Ca(2+) regulation that exists between the biochemical and mechanical cycles of the actin-myosin motor. ..
  31. Mwangi J, Ofner C. Crosslinked gelatin matrices: release of a random coil macromolecular solute. Int J Pharm. 2004;278:319-27 pubmed
    ..These results support the threshold concept of solute size relative to matrix mesh size for release of a flexible, random coil macromolecular solute from a hydrogel. ..
  32. Liu G, Wang J, Lea S, Lin Y. Bioassay labels based on apoferritin nanovehicles. Chembiochem. 2006;7:1315-9 pubmed
  33. Kaneko K, Morelli A, Wang Z, Thomson A. Alloantigen presentation by ethylcarbodiimide-treated dendritic cells induces T cell hyporesponsiveness, and prolongs organ graft survival. Clin Immunol. 2003;108:190-8 pubmed
    ..The effects are not associated with sparing of Th2 responses, but may reflect inhibitory effects of apoptotic donor DC on host immune reactivity. ..
  34. Okumura A, Nagao R, Suzuki T, Yamagoe S, Iwai M, Nakazato K, et al. A novel protein in Photosystem II of a diatom Chaetoceros gracilis is one of the extrinsic proteins located on lumenal side and directly associates with PSII core components. Biochim Biophys Acta. 2008;1777:1545-51 pubmed publisher
    ..gracilis PSII. ..
  35. Shen C, Yang Y, Liu B. Effects of large-area irradiated laser phototherapy on peripheral nerve regeneration across a large gap in a biomaterial conduit. J Biomed Mater Res A. 2013;101:239-52 pubmed publisher
    ..Thus, these assessments demonstrate that LLL therapy can accelerate the repair of a transected peripheral nerve in rats after being bridged with EGT conduit...
  36. Krishnamoorthy G, Sehgal P, Mandal A, Sadulla S. Novel collagen scaffolds prepared by using unnatural D-amino acids assisted EDC/NHS crosslinking. J Biomater Sci Polym Ed. 2013;24:344-64 pubmed publisher
  37. Choi D, Kang S, Kim S, Gobaa S, Park B, Kim I, et al. Growth factors-loaded stents modified with hyaluronic acid and heparin for induction of rapid and tight re-endothelialization. Colloids Surf B Biointerfaces. 2016;141:602-610 pubmed publisher
    ..This study suggested that growth factor loaded stent modified by HA and heparin provided the advantage to rapid and tight restoration of endothelium. ..
  38. Kerr K, Hedstrom L. The roles of conserved carboxylate residues in IMP dehydrogenase and identification of a transition state analog. Biochemistry. 1997;36:13365-73 pubmed
    ..This observation indicates that MMP is a transition state analog for the IMPDH reaction. ..
  39. Malcor J, Bax D, Hamaia S, Davidenko N, Best S, Cameron R, et al. The synthesis and coupling of photoreactive collagen-based peptides to restore integrin reactivity to an inert substrate, chemically-crosslinked collagen. Biomaterials. 2016;85:65-77 pubmed publisher
    ..The method we describe has wide application, beyond collagen films or scaffolds, since the photoreactive diazirine will react with many organic carbon skeletons. ..
  40. Futami J, Maeda T, Kitazoe M, Nukui E, Tada H, Seno M, et al. Preparation of potent cytotoxic ribonucleases by cationization: enhanced cellular uptake and decreased interaction with ribonuclease inhibitor by chemical modification of carboxyl groups. Biochemistry. 2001;40:7518-24 pubmed
  41. Angele P, Abke J, Kujat R, Faltermeier H, Schumann D, Nerlich M, et al. Influence of different collagen species on physico-chemical properties of crosslinked collagen matrices. Biomaterials. 2004;25:2831-41 pubmed
    ..This study also indicates that results obtained for scaffolds based on a certain collagen species may not be transferable to scaffolds based on another, because of the differing physico-chemical properties. ..
  42. Schulz D, Ihling C, Clore G, Sinz A. Mapping the topology and determination of a low-resolution three-dimensional structure of the calmodulin-melittin complex by chemical cross-linking and high-resolution FTICRMS: direct demonstration of multiple binding modes. Biochemistry. 2004;43:4703-15 pubmed
    ..This integrated approach, exemplified for mapping the topology of the calmodulin-melittin complex, is likely to have wide-ranging implications for structural studies on protein-protein interactions. ..
  43. Evran S, Telefoncu A. Modification of porcine pancreatic lipase with Z-proline. Prep Biochem Biotechnol. 2005;35:191-201 pubmed
    ..After the modification, porcine pancreatic lipase was found to have new physicochemical characteristics, such as optimum alkaline pH stability and thermal stability at elevated temperatures. ..
  44. Hupp C, Tepe J. 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride-mediated oxazole rearrangement: gaining access to a unique marine alkaloid scaffold. J Org Chem. 2009;74:3406-13 pubmed publisher
    ..Herein, we report the scope of this novel rearrangement and the proposed mechanism and showcase its utility through the total synthesis of a marine alkaloid from D. grossularia and two analogues. ..
  45. Fan L, Cao M, Gao S, Wang T, Wu H, Peng M, et al. Preparation and characterization of sodium alginate modified with collagen peptides. Carbohydr Polym. 2013;93:380-5 pubmed publisher
    ..The improved functionalities of the derivative might be explained by peptides characteristics. ..
  46. Bedran Russo A, Karol S, Pashley D, Viana G. Site specific properties of carious dentin matrices biomodified with collagen cross-linkers. Am J Dent. 2013;26:244-8 pubmed
    ..05). An average increase of 30-fold, 2-fold and 2.2-fold of the reduced modulus of elasticity was observed following treatments of the three dentin zones with proanthocyanidin, carbodiimide and glutaraldehyde, respectively. ..
  47. Needs P, Rigby N, Ring S, MacDougall A. Specific degradation of pectins via a carbodiimide-mediated Lossen rearrangement of methyl esterified galacturonic acid residues. Carbohydr Res. 2001;333:47-58 pubmed
    ..Analysis of oligomers so generated discloses the pattern of contiguous nonesterification in a variety of pectins of differing degrees of esterification. Other potential applications are described. ..
  48. Lee Y, Kwon O, Yoon Y, Ryu K. Immobilization of horseradish peroxidase on multi-wall carbon nanotubes and its electrochemical properties. Biotechnol Lett. 2006;28:39-43 pubmed
    ..Cyclic voltammetry of the enzyme electrode confirmed electron transfer between the immobilized HRP and the electrode in the presence of H2O2 but without an added mediator or a reducing substrate. ..
  49. Semin B, Seibert M. A carboxylic residue at the high-affinity, Mn-binding site participates in the binding of iron cations that block the site. Biochim Biophys Acta. 2006;1757:189-97 pubmed
  50. Seno K, Matumura K, Oshima M, Motomizu S. Spectrophotometric determination of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow injection analysis. Anal Sci. 2008;24:505-8 pubmed
    ..HCl concentration that remained after the anhydration of phthalic acid in water, esterification of acetic acid in methanol or dehydration condensation of malonic acid and ethylenediamine in water. ..
  51. Panchagnula V, Kumar C, Rusling J. Ultrathin layered myoglobin-polyion films functional and stable at acidic pH values. J Am Chem Soc. 2002;124:12515-21 pubmed
    ..The pH-dependent catalytic reduction of trichloracetic acid was faster in 0.1 M HCl than in the medium pH range. ..
  52. Xu Z, Jayaseharan J, Marchant R. Synthesis and characterization of oligomaltose-grafted lipids with application to liposomes. J Colloid Interface Sci. 2002;252:57-65 pubmed
    ..The glycolipids demonstrated the ability for sterically stabilizing liposome dispersions, as determined by turbidity measurements. ..
  53. Christiaens P, Vermeeren V, Wenmackers S, Daenen M, Haenen K, Nesladek M, et al. EDC-mediated DNA attachment to nanocrystalline CVD diamond films. Biosens Bioelectron. 2006;22:170-7 pubmed
    ..The linking method after the fatty acid attachment can easily be applied to other biomolecules like antibodies and enzymes. ..
  54. Yu R, He M, Shi H, Liu J, Guo J, Wang N, et al. [Conjugation and immunogenic evaluation of complete immunogen for the small molecular environmental pollutants 2,4-D]. Huan Jing Ke Xue. 2006;27:146-50 pubmed
    ..It could be used as the immunogen for the preparation monoclonal antibody. ..
  55. Mann D, McKnight T, Melechko A, Simpson M, Sayler G. Quantitative analysis of EDC-condensed DNA on vertically aligned carbon nanofiber gene delivery arrays. Biotechnol Bioeng. 2007;97:680-8 pubmed
    ..This decrease could be explained by the presence of DNA which is non-tethered to all samples and released during the assay. This investigation shows the applicability of these methods for monitoring DNA immobilization techniques. ..
  56. van Wachem P, Plantinga J, Wissink M, Beernink R, Poot A, Engbers G, et al. In vivo biocompatibility of carbodiimide-crosslinked collagen matrices: Effects of crosslink density, heparin immobilization, and bFGF loading. J Biomed Mater Res. 2001;55:368-78 pubmed
    ..It is concluded that the results of this study justify further evaluation of bFGF preloaded, heparin immobilized EN14 collagen, as a matrix for endothelial cell seeding in experimental animals. ..
  57. Faijes M, Pérez X, Pérez O, Planas A. Glycosynthase activity of Bacillus licheniformis 1,3-1,4-beta-glucanase mutants: specificity, kinetics, and mechanism. Biochemistry. 2003;42:13304-18 pubmed
  58. Li D, Tang H, Speicher D. A structural model of the erythrocyte spectrin heterodimer initiation site determined using homology modeling and chemical cross-linking. J Biol Chem. 2008;283:1553-62 pubmed
    ..This model supports the hypothesis that initial docking of the correct alpha and beta repeats from among many very similar repeats in both subunits is driven primarily by long range electrostatic interactions. ..
  59. Göbel C, Schuster B, Baurecht D, Sleytr U, Pum D. S-layer templated bioinspired synthesis of silica. Colloids Surf B Biointerfaces. 2010;75:565-72 pubmed publisher
    ..yH2O on the S-layer. The silica surface concentrations on the S-layer was 4 x 10(-9) to 2 x 10(-8) mol cm(-2) depending on the modification of the protein layer and corresponded to 4-21 monolayers of SiO2. ..
  60. Calejo M, Almeida A, Fernandes A. Exploring a new jellyfish collagen in the production of microparticles for protein delivery. J Microencapsul. 2012;29:520-31 pubmed publisher
    ..In summary, the work herein described shows the potential use of a marine collagen in the production of microparticles for the controlled release of therapeutic proteins. ..
  61. Pliszka B, Karczewska E, Wawro B. Nucleotide-induced movements in the myosin head near the converter region. Biochim Biophys Acta. 2000;1481:55-62 pubmed
    ..These results indicate that the two regions of the heavy chain that are relatively distant in nucleotide-free skeletal S1 [Rayment et al. (1993) Science 261, 50-58] can potentially interact upon addition of nucleotide. ..
  62. Schaffitzel E, Rudiger S, Bukau B, Deuerling E. Functional dissection of trigger factor and DnaK: interactions with nascent polypeptides and thermally denatured proteins. Biol Chem. 2001;382:1235-43 pubmed
    ..These data indicate that TF and DnaK show strong differences in their ability to prevent aggregation of denatured proteins and to associate with native like substrates, but share the ability to associate with nascent polypeptides. ..
  63. Wrobel N, Schinkinger M, Mirsky V. A novel ultraviolet assay for testing side reactions of carbodiimides. Anal Biochem. 2002;305:135-8 pubmed
    ..The results can be used to optimize carbodiimide-mediated reactions of peptide bond formation in organic chemistry or peptide synthesis or during immobilization or cross-linking of biological molecules. ..
  64. Zhao C, Sun Y, Amadio P, Tanaka T, Ettema A, An K. Surface treatment of flexor tendon autografts with carbodiimide-derivatized hyaluronic Acid. An in vivo canine model. J Bone Joint Surg Am. 2006;88:2181-91 pubmed gelatin may provide surgeons with a new and useful method to improve the quality of tendon graft surgery. ..
  65. Seto A, GATT C, Dunn M. Radioprotection of tendon tissue via crosslinking and free radical scavenging. Clin Orthop Relat Res. 2008;466:1788-95 pubmed publisher
    ..Of the options studied, the data suggest EDC precrosslinking or glucose treatment provides the best maintenance of native tendon properties after exposure to ionizing irradiation. ..
  66. Walker R. ncd and kinesin motor domains interact with both alpha- and beta-tubulin. Proc Natl Acad Sci U S A. 1995;92:5960-4 pubmed
    ..These results indicate that kinesin family motors of opposite polarity interact with both tubulin monomers and support a model in which some portion of each protein's motor domain overlaps adjacent alpha- and beta-tubulin subunits. ..
  67. Yu X, Cheng M, Chen H. [Methods for the pre-treatment of biological tissues for vascular scaffold]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2004;21:476-81 pubmed
    ..Meanwhile, dye-mediated photooxidation crosslink is also a promising pre-treatment which should be widely applied in vascular scaffold. ..
  68. Hu J, Liu X, Hughes D, Esteva F, Liu B, Chandra J, et al. Herceptin conjugates linked by EDC boost direct tumor cell death via programmed tumor cell necrosis. PLoS ONE. 2011;6:e23270 pubmed publisher
    ..In summary, the Herceptin trimer reported herein harbors great potential for overcoming tumor cell resistance to Herceptin treatment. ..
  69. Ghirardi M, Lutton T, Seibert M. Effects of carboxyl amino acid modification on the properties of the high-affinity, manganese-binding site in photosystem II. Biochemistry. 1998;37:13559-66 pubmed
    ..At least one of the two histidyl ligands (either His337 on D1 or another unidentified histidyl) that bind nonphotooxidizable, high-affinity Mn2+ also binds Zn2+ and Co2+. ..
  70. Pieper J, Oosterhof A, Dijkstra P, Veerkamp J, van Kuppevelt T. Preparation and characterization of porous crosslinked collagenous matrices containing bioavailable chondroitin sulphate. Biomaterials. 1999;20:847-58 pubmed
    ..It is concluded that the use of ethanol during EDC crosslinking, offers an elegant means for the preparation of defined porous collagenous matrices containing bioavailable, covalently attached CS. ..
  71. Pieper J, Hafmans T, Veerkamp J, van Kuppevelt T. Development of tailor-made collagen-glycosaminoglycan matrices: EDC/NHS crosslinking, and ultrastructural aspects. Biomaterials. 2000;21:581-93 pubmed
    ..Ultrastructural similarities between artificially engineered scaffolds and their possible application site may favor the use of specific collagen-GAG matrices in tissue engineering. ..
  72. Garnier C, Barbier P, Devred F, Rivas G, Peyrot V. Hydrodynamic properties and quaternary structure of the 90 kDa heat-shock protein: effects of divalent cations. Biochemistry. 2002;41:11770-8 pubmed
    ..This mechanism involved the C-terminal part of the protein since C-terminally truncated Hsp90 did not oligomerize in the presence of divalent cations. ..
  73. Martin B, Karczewska E, Pliszka B. Effect of nucleotide on interaction of the 567-578 segment of myosin heavy chain with actin. Biochim Biophys Acta. 2006;1764:217-22 pubmed
    ..The observed nucleotide-dependent changes of the actin-subfragment 1 interface indicate that the 567-578 residue loop of skeletal muscle myosin participates in the communication between the nucleotide and actin binding sites. ..
  74. Tanaka T, Zhao C, Sun Y, Zobitz M, An K, Amadio P. The effect of carbodiimide-derivatized hyaluronic acid and gelatin surface modification on peroneus longus tendon graft in a short-term canine model in vivo. J Hand Surg Am. 2007;32:876-81 pubmed
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