dimethyl suberimidate


Summary: The methyl imidoester of suberic acid used to produce cross links in proteins. Each end of the imidoester will react with an amino group in the protein molecule to form an amidine.

Top Publications

  1. Gotte G, Libonati M. Two different forms of aggregated dimers of ribonuclease A. Biochim Biophys Acta. 1998;1386:106-12 pubmed
    ..The fast migrating dimer in non-denaturing gel electrophoresis is able to degrade double-stranded poly(A).poly(U) more efficiently than the other, and the two forms are found in a ratio of about 3:1. ..
  2. Patel S, Hingorani M. Oligomeric structure of bacteriophage T7 DNA primase/helicase proteins. J Biol Chem. 1993;268:10668-75 pubmed
    ..Chemical cross-linking of 4A' and 4B proteins with dimethyl suberimidate resulted in cross-linked species ranging from dimers to hexamers and beyond...
  3. Tibbetts A, Appling D. Characterization of two 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase isozymes from Saccharomyces cerevisiae. J Biol Chem. 2000;275:20920-7 pubmed
    ..Ade16p was observed to be more abundant in cells grown on nonfermentable carbon sources than in glucose-grown cells, suggesting a role for this isozyme in respiration or sporulation. ..
  4. Kalacheva N, Konovalova O, Nalimov D, Salakhov M, Il inskaia O, Kurinenko B. [Inhibition of functional activity of macrophages in vitro by dimer RNase Bacillus intermedius]. Tsitologiia. 2008;50:487-91 pubmed
    ..The role of membrane properties modification in the inhibition effect of RNase dimers on the functional activities of macrophages is discussed. ..
  5. Martinez T, Wheeler D. Identification of two storage hexamers in the ant, Camponotus festinatus: accumulation in adult queenless workers. Insect Biochem Mol Biol. 1993;23:309-17 pubmed
    ..However, with respect to their amino acid composition, they can not be classified as arylphorins. ..
  6. Roth M, Welti R. Arrangement of phosphatidylethanolamine molecular species in Escherichia coli membranes and reconstituted lipids as determined by dimethyl suberimidate cross-linking of nearest neighbor lipids. Biochim Biophys Acta. 1994;1190:91-8 pubmed
    ..However, a consistent, slight tendency of divaccenoyl species to pair with like species as compared to pairing with the second most common species, vaccenoyl, palmitoleoyl PE, was noted in both extracted lipids and in whole cells. ..
  7. Watty A, Weise C, Dreger M, Franke P, Hucho F. The accessible surface of the nicotinic acetylcholine receptor. Identification by chemical modification and cross-linking with 14C-dimethyl suberimidate. Eur J Biochem. 1998;252:222-8 pubmed
    ..electric tissue we modified and cross-linked lysine residues with the agonistic bifunctional reagent [14C]dimethyl suberimidate. This reagent labels exposed lysine residues, especially those located near the ligand-binding site, and ..
  8. Lewendon A, Ellis J, Shaw W. Structural and mechanistic studies of galactoside acetyltransferase, the Escherichia coli LacA gene product. J Biol Chem. 1995;270:26326-31 pubmed
    ..GAT was shown to be a trimeric acetyltransferase by cross-linking with dimethyl suberimidate. Fluorometric analysis of coenzyme A binding showed that there is a fluorescence quench associated with ..
  9. Bonnefoy E, Rouviere Yaniv J. HU and IHF, two homologous histone-like proteins of Escherichia coli, form different protein-DNA complexes with short DNA fragments. EMBO J. 1991;10:687-96 pubmed
    ..coli (IHF) form different structures with the same DNA fragments. Moreover, HU seems to enhance the DNA-binding capacity of IHF to a DNA fragment which does not contain its consensus sequence. ..

More Information


  1. Font B, Eichenberger D, Goldschmidt D, Vial C. Interaction of creatine kinase and hexokinase with the mitochondrial membranes, and self-association of creatine kinase: crosslinking studies. Mol Cell Biochem. 1987;78:131-40 pubmed
    ..These results proved that the polymeric form of CKm was an octamer. ..
  2. Munshi C, Baumann C, Levitt D, Bloomfield V, Lee H. The homo-dimeric form of ADP-ribosyl cyclase in solution. Biochim Biophys Acta. 1998;1388:428-36 pubmed
    ..These results indicate the cyclase is a non-covalent dimer in solution. ..
  3. Rosenthal R, Rodwell V. Purification and characterization of the heteromeric transcriptional activator MvaT of the Pseudomonas mevalonii mvaAB operon. Protein Sci. 1998;7:178-84 pubmed
    ..P16 appears to be comprised of at least 128 aminoacyl residues having a predicted molecular mass of 14.3 kDa. ..
  4. Sharma K, Ortwerth B. Effect of cross-linking on the chaperone-like function of alpha crystallin. Exp Eye Res. 1995;61:413-21 pubmed
    ..These data suggest that the age-related cross-linking, which restricts the molecular flexibility of alpha-crystallin decreases its chaperone-like function. ..
  5. Ueno H, Harrington W. Cross-bridge movement and the conformational state of the myosin hinge in skeletal muscle. J Mol Biol. 1981;149:619-40 pubmed
  6. Nakamura S, Sakurai T, Nonomura Y. Determination of the native subunit pattern of gizzard tropomyosin using antibodies specific to each subunit. J Biochem. 1991;109:758-62 pubmed
    ..For the cross-linking experiment we used dimethyl suberimidate, an amino group-specific cross-linker, in the presence of dithiothreitol to avoid artificial oxidative ..
  7. Husic H, Hsieh S, Berrier A. Effect of dithiothreitol on the catalytic activity, quaternary structure and sulfonamide-binding properties of an extracellular carbonic anhydrase from Chlamydomonas reinhardtii. Biochim Biophys Acta. 1991;1078:35-42 pubmed
    ..Chemical cross-linking of the enzyme with dimethyl suberimidate revealed the existence of oligomers containing up to three large subunits and at least two small subunits...
  8. Nishino T, Amaya Y, Kawamoto S, Kashima Y, Okamoto K, Nishino T. Purification and characterization of multiple forms of rat liver xanthine oxidoreductase expressed in baculovirus-insect cell system. J Biochem. 2002;132:597-606 pubmed
  9. Rote K, Hough R, Goldenberg D, Rechsteiner M. Circular pancreatic trypsin inhibitor. A novel substrate for studies on intracellular proteolysis. J Biol Chem. 1989;264:1156-62 pubmed
    ..Thus, increased rates of turnover do not necessarily correlate with greater molecular mass of the substrate. ..
  10. Johnston P, Sudhof T. The multisubunit structure of synaptophysin. Relationship between disulfide bonding and homo-oligomerization. J Biol Chem. 1990;265:8869-73 pubmed
  11. Rouviere Yaniv J, Kjeldgaard N. Native Escherichia coli HU protein is a heterotypic dimer. FEBS Lett. 1979;106:297-300 pubmed
  12. Ingham C. Characterisation of the enzymatic and RNA-binding properties of the Rhodobacter sphaeroides 2.4.1. Rho homologue. Biochim Biophys Acta. 1999;1446:115-25 pubmed
    ..The main differences between Rho factors from different bacteria is in the specificity of RNA binding although this does not appear to be necessarily dependent on the GC bias of target RNA as has been previously suggested. ..
  13. Kastner B, Trotman C, Tate W. Isolation of Escherichia coli release factor-2/ribosome complexes facilitated by efficient crosslinking and sensitive immunodetection of the ligand on the macromolecular particle. Biochem Int. 1987;15:645-52 pubmed
    ..To stabilise the weak 70S.RF-2 complex the two components have been crosslinked using dimethyl suberimidate. A sensitive dot blot procedure employing RF-2 specific antibodies and detecting 0...
  14. Finger L, Richardson J. Stabilization of the hexameric form of Escherichia coli protein rho under ATP hydrolysis conditions. J Mol Biol. 1982;156:203-19 pubmed
  15. Condeelis J, Vahey M, Carboni J, Demey J, Ogihara S. Properties of the 120,000- and 95,000-dalton actin-binding proteins from Dictyostelium discoideum and their possible functions in assembling the cytoplasmic matrix. J Cell Biol. 1984;99:119s-126s pubmed
    ..The properties of 95K are consistent with a role in organizing actin filaments in the cell into lateral arrays that are capable of efficient interaction with myosin to produce force for cell motility. ..
  16. Garcia Perez A, Perez M, Lucas L, Pinilla M, Luque J, Sancho P. Oxygenation capacity of hypotonized and crosslinked rat erythrocytes. Life Sci. 1997;61:445-53 pubmed
    ..subjected to hypotonic-isotonic dialysis, or crosslinking with bifunctional reagents (glutaraldehyde and dimethyl suberimidate hydrochloride) show a high percentage of methemoglobin and decreased oxyhemoglobin content which implies a ..
  17. Tellinghuisen T, Kuhn R. Nucleic acid-dependent cross-linking of the nucleocapsid protein of Sindbis virus. J Virol. 2000;74:4302-9 pubmed
  18. Lummis S, Ellory J, Sattelle D. The cross-linking reagent dimethyl suberimate modifies the target size of an insect nervous system nicotinic acetylcholine receptor. Neurosci Lett. 1988;87:145-50 pubmed
    ..A model is presented to account for the increase in target size and reduction in the number of alpha-[3H]bungarotoxin binding sites in the presence of dimethyl suberimate. ..
  19. Ernst Fonberg M, Williams S, Worsham L. Acyl carrier protein interacts with melittin. Biochim Biophys Acta. 1990;1046:111-9 pubmed
    ..Also, anti-Apis mellifera antibodies which contained antibodies against melittin specifically inhibited the same enzyme system activity relative to non-immune IgG. ..
  20. Ouimet P, Kapoor M. Nucleotide binding and hydrolysis properties of Neurospora crassa cytosolic molecular chaperones, Hsp70 and Hsp80, heat-inducible members of the eukaryotic stress-70 and stress-90 families. Biochem Cell Biol. 1999;77:89-99 pubmed
    ..and Hsp80 by crosslinking with bifunctional reagents: glutaraldehyde, dimethyl adipimidate (DMA), and dimethyl suberimidate (DMS)...
  21. Gotte G, Bertoldi M, Libonati M. Structural versatility of bovine ribonuclease A. Distinct conformers of trimeric and tetrameric aggregates of the enzyme. Eur J Biochem. 1999;265:680-7 pubmed
    ..On the contrary, the activity of the RNase A oligomers, from dimer to pentamer, on yeast RNA and poly(C) (Kunitz assay) is lower than that of monomeric RNase A. ..
  22. Rexin M, Busch W, Segnitz B, Gehring U. Tetrameric structure of the nonactivated glucocorticoid receptor in cell extracts and intact cells. FEBS Lett. 1988;241:234-8 pubmed
    ..Following affinity labeling of the steroid-binding subunit and subsequent cross-linking with dimethyl suberimidate at various times either in cell extracts or in intact cells, a series of labeled bands was detected in SDS ..
  23. Worsham L, Tucker M, Ernst Fonberg M. Behavior of acyl carrier proteins on western blots. Biochim Biophys Acta. 1990;1043:198-202 pubmed
  24. Watanabe Y, Iwakura M, Tokushige M, Eguchi G. Studies on aspartase. VII. Subunit arrangement of Escherichia coli aspartase. Biochim Biophys Acta. 1981;661:261-6 pubmed
    ..of the enzyme was studied by two distinct methods, cross-linking of subunits with a bifunctional reagent, dimethyl suberimidate, and statistical classification of negatively stained electron microscopic images...
  25. Filimonova M, Benedik M, Urazov N, Leshchinskaia I. [Polydispersity of Serratia marcescens nuclease at optimal pH values]. Prikl Biokhim Mikrobiol. 1999;35:20-4 pubmed
    Treatment with dimethyl suberimidate, a cross-linking bifunctional agent, showed that Sm1 and Sm2 nucleases of Serratia marcescens B10M1 are polydisperse in solution and consist of monomers and dimers at the level of pH optimal for the ..
  26. Matyka K, Matyka M, Mroz I, Zalewska Rejdak J, Ciszewski A. An AFM study on mechanical properties of native and dimethyl suberimidate cross-linked pericardium tissue. J Mol Recognit. 2007;20:524-30 pubmed
    Changes in the stiffness of hog pericardium tissue, native and treated with dimethyl suberimidate (DMS), are investigated by atomic force microscopy (AFM). Young's modulus is calculated on the basis of the Hertz-Sneddon model...
  27. Lucas L, Garcia Perez A, Jimeno P, Perez M, Pinilla M, Sancho P, et al. Surface properties of crosslinked erythrocytes as studied by counter-current distribution in aqueous polymer two-phase systems. J Chromatogr B Biomed Appl. 1995;664:137-44 pubmed
    The bifunctional imidoester dimethyl suberimidate hydrochloride can stabilize rat red blood cells (RBCs) by membrane protein crosslinking, and in that way they can be used as carrier systems for exogenous substances...
  28. Calabresi L, Vecchio G, Longhi R, Gianazza E, Palm G, Wadensten H, et al. Molecular characterization of native and recombinant apolipoprotein A-IMilano dimer. The introduction of an interchain disulfide bridge remarkably alters the physicochemical properties of apolipoprotein A-I. J Biol Chem. 1994;269:32168-74 pubmed
  29. Kelly K, Deutscher M. Characterization of Escherichia coli RNase PH. J Biol Chem. 1992;267:17153-8 pubmed
    ..Higher order multimers of RNase PH could be detected by gel filtration at higher protein concentrations and by protein cross-linking. The possible role of RNase PH in tRNA processing is discussed. ..
  30. Wu T, Liu C, Chang Y. A study of the oligomeric state of the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-preferring glutamate receptors in the synaptic junctions of porcine brain. Biochem J. 1996;319 ( Pt 3):731-9 pubmed
    ..Upon incubation of the synaptic junctions with three cross-linking regents, dimethyl adipimidate (DMA), dimethyl suberimidate (DMS) and N-succinimidyl-(4-azidophenyl)-1,3'-dithiopropionate (SADP), AMPA receptor subunits in higher-..
  31. Horiguchi T, Miwa Y, Shigesada K. The quaternary geometry of transcription termination factor rho: assignment by chemical cross-linking. J Mol Biol. 1997;269:514-28 pubmed
    ..Upon reaction with dimethyl suberimidate followed by SDS-polyacrylamide gel electrophoresis, rho protein generated a series of cross-linked ..
  32. Ogawa H, Gomi T, Takusagawa F, Masuda T, Goto T, Kan T, et al. Evidence for a dimeric structure of rat liver serine dehydratase. Int J Biochem Cell Biol. 2002;34:533-43 pubmed
    ..with the previous value (M(r)=64,000); (2) cross-linking experiments of the purified rat SDH with dimethyl suberimidate revealed the existence of a dimeric form by sodium dodecyl sulfate-polyacrylamide gel electrophoresis...
  33. Ali J, Orphanides G, Maxwell A. Nucleotide binding to the 43-kilodalton N-terminal fragment of the DNA gyrase B protein. Biochemistry. 1995;34:9801-8 pubmed
    ..Coumarin drugs inhibit the binding of ADPNP to the 43-kDa fragment, with novobiocin binding to the protein with a stoichiometry of 1:1 and coumermycin binding with a stoichiometry of 0.5:1. ..
  34. Dodson M. Dimethyl suberimidate cross-linking of oligo(dT) to DNA-binding proteins. Bioconjug Chem. 2000;11:876-9 pubmed
    b>Dimethyl suberimidate is a bifunctional reagent that is used for cross-linking the protein components of oligomeric macromolecules...
  35. Lipps G, Stegert M, Krauss G. Thermostable and site-specific DNA binding of the gene product ORF56 from the Sulfolobus islandicus plasmid pRN1, a putative archael plasmid copy control protein. Nucleic Acids Res. 2001;29:904-13 pubmed
    ..2 kJ/mol. Furthermore, recombinant ORF56 proved to be highly thermostable and is able to bind DNA up to 85 degrees C. ..
  36. Muguruma M, Matsumura S, Fukazawa T. Augmentation of alpha-actinin-induced gelation of actin by talin. J Biol Chem. 1992;267:5621-4 pubmed
    ..Treatment of talin with a cross-linking reagent, 1-ethyl-3[3-(dimethylamino)propyl]carbodiimide or dimethyl suberimidate, resulted in the formation of its oligomeric polypeptides...
  37. Pon C, Pawlik R, Gualerzi C. The topographical localization of IF3 on Escherichia coli 30 S ribosomal subunits as a clue to its way of functioning. FEBS Lett. 1982;137:163-7 pubmed
  38. Roth M, Smardo F, Welti R. Analysis of dimeric species derived from the reaction of phosphatidylethanolamine with dimethylsuberimidate. Chem Phys Lipids. 1989;51:39-46 pubmed
    ..Proton nuclear magnetic resonance spectroscopy and chemical analysis implied that the dimers contain an amidine group. ..
  39. Frosini M, Larini A, Ricci L, Lucas L, Gorelli B, Sgaragli G, et al. Effects of autologous, cross-linked erythrocytes on isolated hypoperfused rabbit heart dynamics. Pharmacology. 2012;90:274-80 pubmed publisher
    ..aimed at assessing the effects of either red blood cells (RBC) or RBC cross-linked with the bifunctional dimethyl suberimidate reagent (C-RBC) on contractile force (CFo), heart rate (HR) and coronary flow (CF) of the isolated rabbit ..
  40. Johnson W, Chacko G, Phillips M, Rothblat G. The efflux of lysosomal cholesterol from cells. J Biol Chem. 1990;265:5546-53 pubmed
    ..with acceptors that do not interact with HDL-specific cell surface binding sites (phospholipid vesicles, dimethyl suberimidate cross-linked HDL)...
  41. Koul D, Obeyesekere N, Gutterman J, Mills G, Choubey D. p202 self-associates through a sequence conserved among the members of the 200-family proteins. FEBS Lett. 1998;438:21-4 pubmed
    ..Our observations reported herein raise the possibility that self-association of p202 may provide a mechanism for the regulation of its activity. ..
  42. Jozwiak Z, Schon W, Kraft G, Gartner H. Fluidity alterations induced by chemical modification of erythrocyte membrane proteins. Biochem Int. 1992;28:265-72 pubmed
    ..These results suggest that modified proteins may perturb the interactions of the membrane components and lead to alterations of the membrane organization in the polar region...
  43. Rowell J, Simpson K, Stott K, Watson M, Thomas J. HMGB1-facilitated p53 DNA binding occurs via HMG-Box/p53 transactivation domain interaction, regulated by the acidic tail. Structure. 2012;20:2014-24 pubmed publisher
    ..The incipient amphipathic helix in TAD2 recognizes the concave DNA-binding face of the A box and may be acting as a single-stranded DNA mimic. ..
  44. Pippia P, Cogoli A, Sciola L, Tilloca G, Meloni M, Barni S. [Effect of dimethyladipimidate and dimethylsuberimidate on cell-cell adhesion in rat fibroblasts]. Boll Soc Ital Biol Sper. 1990;66:945-52 pubmed
  45. Hucho F, Methfessel C, Watty A. The role of subunit interfaces for the nicotinic acetylcholine receptor's allosterism. J Physiol Paris. 1998;92:85-8 pubmed
  46. Kilsdonk E, Van Gent T, Dorsman A, van Tol A. Binding of modified high density lipoproteins to endothelial cells: relation with cellular cholesterol efflux?. Atherosclerosis. 1992;97:131-42 pubmed
    ..cells interacted with native apolipoprotein (apo) E-free high density lipoprotein3 (HDL)3 as well as with dimethyl suberimidate-modified HDL3 (DMS-HDL3)...
  47. Carpick B, Graziano V, Schneider D, Maitra R, Lee X, Williams B. Characterization of the solution complex between the interferon-induced, double-stranded RNA-activated protein kinase and HIV-I trans-activating region RNA. J Biol Chem. 1997;272:9510-6 pubmed
    ..Taken together, these data indicate that the PKR activation complex consists of a protein dimer bound cooperatively to one dsRNA molecule. ..
  48. Kaltenbach L, Braaten B, Low D. Specific binding of PapI to Lrp-pap DNA complexes. J Bacteriol. 1995;177:6449-55 pubmed
    ..On the basis of these data, we present a hypothesis in which PapI facilitates the transition between OFF and ON transcription states by binding to Lrp and altering Lrp's affinity for the pap GATC-I and GATC-II regions. ..
  49. Vanhoutte C, Malaisse Lagae F, Malaisse W. D-glucose metabolism in cross-linked pancreatic islets. Biochem Biophys Res Commun. 1995;217:561-5 pubmed
    ..metabolism was investigated in pancreatic islets that underwent cross-linking of intracellular proteins by dimethyl suberimidate. Both the utilization of D-[5-3H]glucose and oxidation of D-[U-14C]glucose were much more severely ..
  50. Senault C, Mahlberg F, Renaud G, Girard Globa A, Chacko G. Effect of apoprotein cross-linking on the metabolism of human HDL3 in rat. Biochim Biophys Acta. 1990;1046:81-8 pubmed
    ..However, results suggest that the binding of HDL to its specific cell surface sites is not necessary for this uptake. ..
  51. de Lumley Woodyear T, Rocca P, Lindsay J, Dror Y, Freeman A, Heller A. Polyacrylamide-based redox polymer for connecting redox centers of enzymes to electrodes. Anal Chem. 1995;67:1332-8 pubmed
    ..16 V (SCE). The GOx electrodes, when made with 140 micrograms/cm2 thick polymer films, were selective for glucose in the presence of physiological concentrations of urate and ascorbate. ..
  52. Vanhoutte C, Malaisse W. D-glucose metabolism in dimethyl suberimidate-treated tumoral pancreatic islet cells. Biochem Mol Biol Int. 1997;41:1209-16 pubmed
    After protein cross-linking by dimethyl suberimidate, tumoral insulin-producing cells of the RINm5F line were either exposed to digitonin for measurement of hexokinase activity in the resulting cell pellet and supernatant, or incubated ..
  53. Roth M, Welti R. Lipid arrangement in fluid model membranes: analysis by cross-linking of phosphatidylethanolamines. Biochim Biophys Acta. 1991;1063:242-6 pubmed
  54. Charulatha V, Rajaram A. Crosslinking density and resorption of dimethyl suberimidate-treated collagen. J Biomed Mater Res. 1997;36:478-86 pubmed
    Collagen was purified from bovine Achilles tendon and crosslinked with dimethyl suberimidate (DMS) and glutaraldehyde (GTA)...
  55. Moshnikova A, Moshnikov S, Afanasyev V, Krotova K, Sadovnikov V, Beletsky I. Cell death induced by chemical homobifunctional cross-linkers. Cross-linker induced apoptosis. Int J Biochem Cell Biol. 2001;33:1160-71 pubmed
    ..to test the apoptosis-inducing and modifying properties of the homobifunctional cross-linkers-dimethyl suberimidate (DMS) and 1,5-bis(succinimido-oxycarbonyloxy)pentane (BSOCOP)...
  56. Ueno H, Harrington W. Cross-linking within the thick filaments of muscle and its effect on contractile force. Biochemistry. 1987;26:3589-96 pubmed
    ..we find that the heads of the myosin molecules are not cross-linked to the thick filament surface by dimethyl suberimidate. The time dependence of cross-linking rod segments within the core was monitored by a disulfide oxidation ..
  57. Yoneyama T, Hatakeyama K. Decameric GTP cyclohydrolase I forms complexes with two pentameric GTP cyclohydrolase I feedback regulatory proteins in the presence of phenylalanine or of a combination of tetrahydrobiopterin and GTP. J Biol Chem. 1998;273:20102-8 pubmed
    ..Phenylalanine was the most potent of the aromatic amino acids. ..
  58. Sheehan H, O Kennedy R, Kilty C. Investigation of the properties of bovine heart creatine kinase cross-linked with dimethyl suberimidate. Biochim Biophys Acta. 1990;1041:141-5 pubmed
    ..7.3.2, ATP: creatine N-phosphotransferase) has been cross-linked with the bifunctional reagent dimethyl suberimidate at several concentrations to yield modified enzyme with enhanced stability towards heat denaturation...
  59. Burt H, Jackson J. Role of membrane proteins in monosodium urate crystal-membrane interactions. II. Effect of pretreatments of erythrocyte membranes with membrane permeable and impermeable protein crosslinking agents. J Rheumatol. 1990;17:1359-63 pubmed
    Intact, human erythrocytes were pretreated with membrane permeable, dimethyl adipimidate (DMA) and dimethyl suberimidate (DMS) and membrane impermeable 3,3' dithiobis (sulfosuccinimidylpropionate) (DTSSP) protein crosslinking agents and ..
  60. Hey K, Lachs C, Raxworthy M, Wood E. Crosslinked fibrous collagen for use as a dermal implant: control of the cytotoxic effects of glutaraldehyde and dimethylsuberimidate. Biotechnol Appl Biochem. 1990;12:85-93 pubmed
    ..3 mM dimedone. [3H]Leucine incorporation by cells exposed to crosslinked collagen treated with these combinations approached 100% of the values recorded with cells exposed to uncrosslinked collagen. ..
  61. Galazka M, Tang M, DeBari V, Kohles J, Lee J, Keil L, et al. Modification of beta 2glycoprotein I by glutardialdehyde. Conformational changes and aggregation accompany exposure of the cryptic autoepitope. Appl Biochem Biotechnol. 1999;76:1-13 pubmed
    ..Thus, by synthesizing an active holoantigen of beta 2 GPI, conditions were defined that are necessary for binding of human autoantibodies. ..
  62. Sancho P, Garcia Perez A, Cuesta A, Pinilla M, Luque J. Surface properties of crosslinked and crosslinked-permeabilized erythrocytes as studied by partitioning in aqueous polymer two-phase systems. Biochem Mol Biol Int. 1993;30:537-45 pubmed
    ..systems have been used to study the surface properties of red blood cells stabilized by crosslinking with dimethyl suberimidate and permeabilized with digitonin...
  63. Krause J, Dudek S, Schwenke K. Changes in interfacial behaviour, emulsifying and foaming properties of faba bean legumin after modification with dimethylsuberimidate. Nahrung. 2000;44:403-6 pubmed
    ..The results support the view that surface activity is generally governed more by molecular flexibility than by surface hydrophobicity. ..
  64. Worsham L, Tucker M, Ernst Fonberg M. Chemical cross-linking and its effect on fatty acid synthetase activity in intact chloroplasts from Euglena gracilis. Biochim Biophys Acta. 1988;963:423-8 pubmed
    ..The acyl-carrier protein concentration in the chloroplast was measured at about 0.24 mM...
  65. Baldwin S, Perham R, Stribling D. Purification and characterization of the class-II D-fructose 1,6-bisphosphate aldolase from Escherichia coli (Crookes' strain). Biochem J. 1978;169:633-41 pubmed
    ..The class-II enzymes from the prokaryote E. coli and the eukaryote yeast show no immunological identity. However, the similarity of their structures suggests that they have evolved from a common ancestor. ..
  66. Holden J, Meredith G, Kelley W. Human adenine phosphoribosyltransferase. Affinity purification, subunit structure, amino acid composition, and peptide mapping. J Biol Chem. 1979;254:6951-5 pubmed
    ..The amino acid composition reveals that 33% of the residues are hydrophobic. ..
  67. Kukolj G, DuBow M. Characterization of the lysogenic repressor (c) from transposable Mu-like bacteriophage D108. Nucleic Acids Res. 1991;19:5949-56 pubmed
    ..Analytical gel filtration and dimethyl suberimidate cross-linking of repressor at 0.1-0...
  68. Kriegel T, Schellenberger W, Kopperschlager G, Hofmann E. Yeast phosphofructokinase: kinetic characterization of a substrate-imprinted enzyme conformation. Biomed Biochim Acta. 1991;50:1159-65 pubmed
  69. Wang Y, Tate S. Oligomeric structure of a renal cystine transporter: implications in cystinuria. FEBS Lett. 1995;368:389-92 pubmed
    ..Our data suggest that the heterodimer is the minimal functional unit of NBAT-mediated amino acid transport and that the NBAT-associated 50 kDa protein could play a role in cystinuria. ..
  70. Cervoni L, Ferraro A, Giartosio A, Wang C, Turano C. RNA polymerase II from wheat germ: a cross-linking study of subunits topography. Arch Biochem Biophys. 1994;311:35-41 pubmed
    ..On the basis of these results a model of the quaternary structure of the enzyme is proposed...
  71. Ausio J, van der Goot F, Buckley J. Physical and chemical characterization of the oligomerization state of the Aeromonas hydrophila lipase/acyltransferase. FEBS Lett. 1993;333:296-300 pubmed
    ..An important change occurs in the apparent partial specific volume of the molecule upon activation. ..
  72. van der Goot F, Ausio J, Wong K, Pattus F, Buckley J. Dimerization stabilizes the pore-forming toxin aerolysin in solution. J Biol Chem. 1993;268:18272-9 pubmed
    ..showed that proaerolysin is a dimer in solution, and this was confirmed by chemical cross-linking with dimethyl suberimidate. Dissociation of proaerolysin with low concentrations of SDS resulted in the loss of tertiary structure, ..
  73. Krupenko S, Kolesnik O, Krupenko N, Strel chyonok O. Organization of the transcortin-binding domain on placental plasma membranes. Biochim Biophys Acta. 1995;1235:387-94 pubmed
    ..Apparently, binding of two dimers results in the formation of the functional form of the transcortin-receptor complex. The possible biological role of such a complex is discussed. ..
  74. Chacko G, Mahlberg F, Johnson W. Cross-linking of apoproteins in high density lipoprotein by dimethylsuberimidate inhibits specific lipoprotein binding to membranes. J Lipid Res. 1988;29:319-24 pubmed
    ..Because of its reduced ability to bind to the specific binding sites, DMS-HDL3 may be useful for studies related to the functional aspects of HDL binding sites. ..
  75. Kahana E, Flood G, Gratzer W. Physical properties of dystrophin rod domain. Cell Motil Cytoskeleton. 1997;36:246-52 pubmed
    ..Soc. Trans. 24:2805] that the dystrophin rod domain lacks the arrangement of sites required for lateral self-association, and that dystrophin, unlike the other known proteins of the spectrin superfamily, may thus exist as a monomer. ..
  76. Moshnikova A, Moshnikov S, Afanas ev V, Krotova K, Sadovnikov V, Beletskiĭ I. [Dimethyl suberimidate as a specific inductor of apoptosis in transformed cells]. Tsitologiia. 2001;43:747-54 pubmed
    ..undertaken to test a possibility to induce and(or) to modify cell death by a homobifunctional cross-linker dimethyl suberimidate (DMS)...
  77. Roth M, Avery R, Welti R. Cross-linking of phosphatidylethanolamine neighbors with dimethylsuberimidate is sensitive to the lipid phase. Biochim Biophys Acta. 1989;986:217-24 pubmed
    ..The ability of dimethylsuberimidate to discriminate between phosphatidylethanolamines in the fluid and gel phases should allow use of this reagent to identify phosphatidylethanolamine species within the gel or fluid lipid phase. ..
  78. Ryan O, Smyth M, Fágáin C. Thermostabilized chemical derivatives of horseradish peroxidase. Enzyme Microb Technol. 1994;16:501-5 pubmed
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