carbonyl cyanide p trifluoromethoxyphenylhydrazone


Summary: A proton ionophore that is commonly used as an uncoupling agent in biochemical studies.

Top Publications

  1. Wyatt C, Buckler K. The effect of mitochondrial inhibitors on membrane currents in isolated neonatal rat carotid body type I cells. J Physiol. 2004;556:175-91 pubmed
    ..These observations suggest that there is a close link between oxygen sensing and mitochondrial function in type I cells. Mechanisms that could account for this link and the actions of mitochondrial inhibitors are discussed...
  2. Henrich M, Buckler K. Effects of anoxia, aglycemia, and acidosis on cytosolic Mg2+, ATP, and pH in rat sensory neurons. Am J Physiol Cell Physiol. 2008;294:C280-94 pubmed
    ..The consequences of these events therefore need to be considered in the context of sensory neuron function in ischemia. ..
  3. Huang S. Development of a high throughput screening assay for mitochondrial membrane potential in living cells. J Biomol Screen. 2002;7:383-9 pubmed
    ..This assay may be used in screens for drugs to treat metabolic disorders such as obesity and diabetes, as well as cancer and neurodegenerative diseases. ..
  4. Kenyon J, Goff H. Temperature dependencies of Ca2+ current, Ca(2+)-activated Cl- current and Ca2+ transients in sensory neurones. Cell Calcium. 1998;24:35-48 pubmed
  5. Dufer M, Krippeit Drews P, Drews G. Inhibition of mitochondrial function affects cellular Ca2+ handling in pancreatic B-cells. Pflugers Arch. 2002;444:236-43 pubmed
    ..Thus, it is concluded that intact mitochondrial function is a prerequisite for regular Ca2+ handling in B-cells. ..
  6. Cheeti S, Warrier B, Lee C. The role of monocarboxylate transporters in uptake of lactic acid in HeLa cells. Int J Pharm. 2006;325:48-54 pubmed
    ..MCT isoforms 1, 5 and 6 in HeLa cells were identified by RT-PCR. HeLa cell line can be used as an effective screening tool for intravaginally administered drugs targeted toward MCT. ..
  7. Kim M, Kang K, Kim C, Choi S. Real-time imaging of mitochondria in transgenic zebrafish expressing mitochondrially targeted GFP. Biotechniques. 2008;45:331-4 pubmed publisher
    ..It could also facilitate study of the pathogenesis of apoptosis-related diseases. ..
  8. Fujiwara A, Yasumasu I. Does the respiratory rate in sea urchin embryos increase during early development without proliferation of mitochondria?. Dev Growth Differ. 1997;39:179-89 pubmed
    ..Acceptor control of respiration is experimentally reproduced in isolated mitochondria by making adenine nucleotide levels as those levels in the pre-hatching period...
  9. Dedkova E, Ji X, Lipsius S, Blatter L. Mitochondrial calcium uptake stimulates nitric oxide production in mitochondria of bovine vascular endothelial cells. Am J Physiol Cell Physiol. 2004;286:C406-15 pubmed
    ..This indicates the presence of a mitochondria-specific NOS that can provide a fast local modulatory effect of NO on cell respiration, membrane potential, and apoptosis. ..

More Information

Publications110 found, 100 shown here

  1. Bolaños P, Guillen A, Rojas H, Boncompagni S, Caputo C. The use of CalciumOrange-5N as a specific marker of mitochondrial Ca2+ in mouse skeletal muscle fibers. Pflugers Arch. 2008;455:721-31 pubmed
    ..Our results show the loss of Ca(2+) from mitochondria when DeltaPsi(m) is depolarised and demonstrate the usefulness of CaOr-5N to mark mitochondrial [Ca(2+)](m). ..
  2. Bragadin M, Cima F, Ballarin L, Manente S. Irgarol inhibits the synthesis of ATP in mitochondria from rat liver. Chemosphere. 2006;65:1898-903 pubmed
    ..The results indicate that Irgarol inhibits the ATP synthesis. The analysis of the various steps involved in the ATP synthesis suggests that the inhibition is due to the opening of small-size pores. ..
  3. Seki S, Itagaki S, Kobayashi M, Hirano T, Iseki K. Amiodarone increases the accumulation of DEA in a human alveolar epithelium-derived cell line. Biol Pharm Bull. 2008;31:1449-52 pubmed
    ..5+/-3.0 microM). This further DEA accumulation by AMD might be a factor responsible for the greater accumulation of DEA than that of AMD in the lung in long-term AMD-treated patients. ..
  4. Giovannucci D, Hlubek M, Stuenkel E. Mitochondria regulate the Ca(2+)-exocytosis relationship of bovine adrenal chromaffin cells. J Neurosci. 1999;19:9261-70 pubmed
  5. Malli R, Frieden M, Osibow K, Graier W. Mitochondria efficiently buffer subplasmalemmal Ca2+ elevation during agonist stimulation. J Biol Chem. 2003;278:10807-15 pubmed
    ..Thus, superficial mitochondria and ER generate local domains of low and high Ca(2+) allowing simultaneous activation of BK(Ca) and CCE, despite their opposite Ca(2+) sensitivity. ..
  6. Feeney C, Pennefather P, Gyulkhandanyan A. A cuvette-based fluorometric analysis of mitochondrial membrane potential measured in cultured astrocyte monolayers. J Neurosci Methods. 2003;125:13-25 pubmed
    ..The merits and pitfalls of these different potentiometric dyes for monitoring Deltapsi(M) are discussed. ..
  7. Li C, Fox C, Master S, Bindokas V, Chodosh L, Thompson C. Bcl-X(L) affects Ca(2+) homeostasis by altering expression of inositol 1,4,5-trisphosphate receptors. Proc Natl Acad Sci U S A. 2002;99:9830-5 pubmed
    ..These results suggest that even under non-apoptotic conditions, expression of Bcl-2-family proteins influences a signaling network that links changes in mitochondrial metabolism to alterations in nuclear gene expression. ..
  8. von Lewinski F, Keller B. Mitochondrial Ca2+ buffering in hypoglossal motoneurons from mouse. Neurosci Lett. 2005;380:203-8 pubmed
    ..Taken together, our findings further support the important role of mitochondria as regulators of Ca2+ homeostasis in motoneurons. ..
  9. Tonshin A, Lobysheva N, Yaguzhinsky L, Bezgina E, Moshkov D, Nartsissov Y. Effect of the inhibitory neurotransmitter glycine on slow destructive processes in brain cortex slices under anoxic conditions. Biochemistry (Mosc). 2007;72:509-17 pubmed
    ..The succinate oxidase fragment of the mitochondrial respiratory chain proved especially resistant; it retains activity under anoxia for more than 72 h. A possible mechanism of the effect of high glycine concentrations is discussed. ..
  10. Linetska M, Storchak L, Tarasenko A, Himmelreich N. Involvement of membrane GABA transporter in alpha-latrotoxin-stimulated [3H]GABA release. Neurochem Int. 2004;44:303-12 pubmed
    ..The incomplete inhibition with NO-711 of the tardy step of the release evoked by nanomolar toxin concentrations suggests the participation not only of the GABA transporters. ..
  11. Inoki Y, Miura T, Kajimoto T, Kawase M, Kawase Y, Yoshida Y, et al. Ganglioside GD3 and its mimetics induce cytochrome c release from mitochondria. Biochem Biophys Res Commun. 2000;276:1210-6 pubmed
    ..In contrast, gangliosides GM1, GM2, and GM3 did not induce cytochrome c release. These results indicate that two sialo residues must play an important role in the induction of cytochrome c release by gangliosides. ..
  12. Evans N, Gnudi L, Rolinski O, Birch D, Pickup J. Non-invasive glucose monitoring by NAD(P)H autofluorescence spectroscopy in fibroblasts and adipocytes: a model for skin glucose sensing. Diabetes Technol Ther. 2003;5:807-16 pubmed
    ..We conclude that glucose can be sensed non-invasively by cellular fluorescence changes in fibroblasts and adipocytes. This is a model for the further exploration of fluorescence-based non-invasive metabolic monitoring in human diabetes. ..
  13. Fonteriz R, de la Fuente S, Moreno A, Lobaton C, Montero M, Alvarez J. Monitoring mitochondrial [Ca(2+)] dynamics with rhod-2, ratiometric pericam and aequorin. Cell Calcium. 2010;48:61-9 pubmed publisher
    ..In addition, cells loaded with low concentrations of rhod-2 suffered large changes in mitochondrial morphology after light excitation. Our results suggest that [Ca(2+)](M) data obtained with these dyes should be taken with care. ..
  14. Coatesworth W, Bolsover S. Spatially organised mitochondrial calcium uptake through a novel pathway in chick neurones. Cell Calcium. 2006;39:217-25 pubmed
  15. Sergeant G, Bradley E, Thornbury K, McHale N, Hollywood M. Role of mitochondria in modulation of spontaneous Ca2+ waves in freshly dispersed interstitial cells of Cajal from the rabbit urethra. J Physiol. 2008;586:4631-42 pubmed publisher
    ..These data suggest that spontaneous Ca(2+) waves in urethral ICC are regulated by buffering of cytoplasmic Ca(2+) by mitochondria. ..
  16. Huser J, Blatter L. Fluctuations in mitochondrial membrane potential caused by repetitive gating of the permeability transition pore. Biochem J. 1999;343 Pt 2:311-7 pubmed
    ..In contrast, loss of matrix metabolites, swelling and other structural damage of the organelle render prolonged openings of the transition pore deleterious to mitochondria and to the cell. ..
  17. Moon Y, Lee K, Park J, Geum D, Kim K. Mitochondrial membrane depolarization and the selective death of dopaminergic neurons by rotenone: protective effect of coenzyme Q10. J Neurochem. 2005;93:1199-208 pubmed
    ..These data suggest that mitochondrial depolarization may play a crucial role in rotenone-induced selective apoptosis in rat primary dopaminergic neurons. ..
  18. Rolo A, Palmeira C, Cortopassi G. Biosensor plates detect mitochondrial physiological regulators and mutations in vivo. Anal Biochem. 2009;385:176-8 pubmed publisher
    ..We describe a high-throughput method for measurement of mitochondrial oxygen consumption in living cells, based on the Becton-Dickinson Biosensor plates. ..
  19. Territo P, Mootha V, French S, Balaban R. Ca(2+) activation of heart mitochondrial oxidative phosphorylation: role of the F(0)/F(1)-ATPase. Am J Physiol Cell Physiol. 2000;278:C423-35 pubmed
    ..This type of parallel control of both intermediary metabolism and ATP synthesis may provide a mechanism of altering ATP production rates with minimal changes in the high-energy intermediates as observed in vivo...
  20. Munoz F, Martin M, Salinas M, Fando J. Carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) induces initiation factor 2 alpha phosphorylation and translation inhibition in PC12 cells. FEBS Lett. 2001;492:156-9 pubmed
    ..No changes in the PKR endoplasmic reticulum-related kinase or eIF2 alpha phosphatase were found. Translational regulation may play a significant role in the process triggered by mitochondrial calcium mobilization. ..
  21. Hickey C, Geiger J, Groten C, Magoski N. Mitochondrial Ca2+ activates a cation current in Aplysia bag cell neurons. J Neurophysiol. 2010;103:1543-56 pubmed publisher
    ..Employing Ca(2+) from mitochondria, rather than the more common endoplasmic reticulum, represents a diversification of the mechanisms that influence neuronal activity. ..
  22. Martins O, Tuena de Gomez Puyou M, Gomez Puyou A. Pre-steady-state studies of the adenosine triphosphatase activity of coupled submitochondrial particles. Regulation by ADP. Biochemistry. 1988;27:7552-8 pubmed
    ..This inhibitory ADP remained bound to the enzyme after several thousand turnovers. Apparently, it is possible to observe maximal rates of hydrolysis only in the first few catalytic cycles of the enzyme...
  23. Zavilgelsky G, Kotova V, Mazhul M, Manukhov I. Role of Hsp70 (DnaK-DnaJ-GrpE) and Hsp100 (ClpA and ClpB) chaperones in refolding and increased thermal stability of bacterial luciferases in Escherichia coli cells. Biochemistry (Mosc). 2002;67:986-92 pubmed
  24. Ørtenblad N, Stephenson D. A novel signalling pathway originating in mitochondria modulates rat skeletal muscle membrane excitability. J Physiol. 2003;548:139-45 pubmed
  25. Xie X, Gao S, Gu W, Pan G, Wang G. Desiccation induces accumulations of antheraxanthin and zeaxanthin in intertidal macro-alga Ulva pertusa (Chlorophyta). PLoS ONE. 2013;8:e72929 pubmed publisher
    ..Considering the pleiotropic effects of Ax and Zx, accumulated Ax and Zx may function in protecting thylakoid membrane and enhancing thermal quenching during emersion in air. ..
  26. Chen C, Chen S. Lactate transport and glycolytic activity in the freshly isolated rabbit cornea. Arch Biochem Biophys. 1990;276:70-6 pubmed
    ..These findings suggest that the ionophore-mediated lactate efflux favors the reduction of low pyruvate concentration in the tissue, rather than parallel increases in glycolytic activity. ..
  27. Luvisetto S, Schemehl I, Canton M, Azzone G. The effect of respiration on the permeability of the mitochondrial membrane to ions. Biochim Biophys Acta. 1994;1186:12-8 pubmed
  28. Galkin M, Venard R, Vaillier J, Velours J, Haraux F. Functional transitions of F0F1-ATPase mediated by the inhibitory peptide IF1 in yeast coupled submitochondrial particles. Eur J Biochem. 2004;271:1963-70 pubmed
    ..The relationship between the different steps of the catalytic cycle, the mechanism of inhibition by IF1 and the interconversion process is discussed. ..
  29. Brustovetsky T, Li V, Brustovetsky N. Stimulation of glutamate receptors in cultured hippocampal neurons causes Ca2+-dependent mitochondrial contraction. Cell Calcium. 2009;46:18-29 pubmed publisher
    ..Consistent with this, in saponin-permeabilized neurons, Ca(2+) caused mitochondrial remodelling which could be prevented by Ru(360). ..
  30. Xiong J, Camello P, Verkhratsky A, Toescu E. Mitochondrial polarisation status and [Ca2+]i signalling in rat cerebellar granule neurones aged in vitro. Neurobiol Aging. 2004;25:349-59 pubmed
  31. Zhou J, Yi J, Fu R, Liu E, Siddique T, Rios E, et al. Hyperactive intracellular calcium signaling associated with localized mitochondrial defects in skeletal muscle of an animal model of amyotrophic lateral sclerosis. J Biol Chem. 2010;285:705-12 pubmed publisher
    ..Our data reveal that mitochondria regulate Ca(2+) signaling in skeletal muscle, and loss of this capacity may contribute to the progression of muscle atrophy in ALS. ..
  32. Jin S, Zhang L, Lian Q, Yao S, Wu P, Zhou X, et al. Close functional coupling between Ca2+ release-activated Ca2+ channels and reactive oxygen species production in murine macrophages. Mediators Inflamm. 2006;2006:36192 pubmed
    ..Conclusions. These findings indicate that the LPS-induced intracellular [Ca(2+)](i) increase depends on the Ca(2+) entry through CRAC channels, and close functional coupling between CRAC and ROS production in murine macrophages. ..
  33. Geiger J, Magoski N. Ca2+-induced Ca2+ release in Aplysia bag cell neurons requires interaction between mitochondrial and endoplasmic reticulum stores. J Neurophysiol. 2008;100:24-37 pubmed publisher
    ..This unique form of CICR may be used by both bag cell neurons, and other neurons, to initiate secretion, activate channels, or induce gene expression. ..
  34. Okorokov L, Silva F, Okorokova Façanha A. Ca(2+) and H+ homeostasis in fission yeast: a role of Ca(2+)/H+ exchange and distinct V-H+-ATPases of the secretory pathway organelles. FEBS Lett. 2001;505:321-4 pubmed
  35. Yang F, He X, Russell J, Lu B. Ca2+ influx-independent synaptic potentiation mediated by mitochondrial Na(+)-Ca2+ exchanger and protein kinase C. J Cell Biol. 2003;163:511-23 pubmed
    ..Inhibition of PKC blocked the TISP as well as mitochondrial Ca2+ release. These results reveal a novel form of synaptic plasticity and suggest a role of PKC in mitochondrial Ca2+ release during synaptic transmission. ..
  36. Ross M, Da Ros T, Blaikie F, Prime T, Porteous C, Severina I, et al. Accumulation of lipophilic dications by mitochondria and cells. Biochem J. 2006;400:199-208 pubmed
    ..Instead, this suggests that it may be possible to form dications within mitochondria that then remain within the cell. ..
  37. Vercesi A, Bernardes C, Hoffmann M, Gadelha F, Docampo R. Digitonin permeabilization does not affect mitochondrial function and allows the determination of the mitochondrial membrane potential of Trypanosoma cruzi in situ. J Biol Chem. 1991;266:14431-4 pubmed
    ..The trypanocidal drug crystal violet, as well as other cationic drugs such as dequalinium, induced a rapid dose-related collapse of the inner mitochondrial membrane potential. ..
  38. Miccadei S, Floridi A. Sites of inhibition of mitochondrial electron transport by cadmium. Chem Biol Interact. 1993;89:159-67 pubmed
  39. Mooren F, Turi S, Gunzel D, Schlue W, Domschke W, Singh J, et al. Calcium-magnesium interactions in pancreatic acinar cells. FASEB J. 2001;15:659-72 pubmed
  40. Dussmann H, Rehm M, Kogel D, Prehn J. Outer mitochondrial membrane permeabilization during apoptosis triggers caspase-independent mitochondrial and caspase-dependent plasma membrane potential depolarization: a single-cell analysis. J Cell Sci. 2003;116:525-36 pubmed
    ..Our results demonstrate that outer mitochondrial membrane permeabilization coordinates the depolarization of both membrane potentials during apoptosis. ..
  41. Grassl S. Facilitated diffusion of urate in avian brush-border membrane vesicles. Am J Physiol Cell Physiol. 2002;283:C1155-62 pubmed
    ..These observations suggest a uniporter-mediated facilitated diffusion of urate as a pathway for passive efflux across the brush border membrane of urate-secreting proximal tubule cells...
  42. Trumper L, Schonfeld P, Augustin W. Flux control coefficients for Pi- and adenine nucleotide translocator in rat liver mitochondria after Fe2+/ascorbate induced oxidative stress. Biomed Biochim Acta. 1989;48:S44-7 pubmed
    ..The data show that the predominant step in the control of oxidative phosphorylation at the site of the consumers must be attributed to the ATP/ADP-exchanger. ..
  43. Behbahani H, Shabalina I, Wiehager B, Concha H, Hultenby K, Petrovic N, et al. Differential role of Presenilin-1 and -2 on mitochondrial membrane potential and oxygen consumption in mouse embryonic fibroblasts. J Neurosci Res. 2006;84:891-902 pubmed
    ..These data point toward a specific role of PS2/gamma-secretase activity for proper mitochondrial function and indicate interplay between PS1 and PS2 in mitochondrial functionality. ..
  44. Tosco M, Orsenigo M, Gastaldi G, Faelli A. pH dependence of Cl/HCO3 exchanger in the rat jejunal enterocyte. Biochim Biophys Acta. 1998;1372:323-30 pubmed
  45. Longo V, Liou L, Valentine J, Gralla E. Mitochondrial superoxide decreases yeast survival in stationary phase. Arch Biochem Biophys. 1999;365:131-42 pubmed
    ..These results raise the possibility that similar processes may occur in higher eukaryotes...
  46. Kang T, Park M, Uhm D. Effects of hypoxia and mitochondrial inhibition on the capacitative calcium entry in rabbit pulmonary arterial smooth muscle cells. Life Sci. 2003;72:1467-79 pubmed
    ..Together, it is suggested that the augmentation of CCE-induced [Ca(2+)]c transients by hypoxia and FCCP reflects a net gain of [Ca(2+)]c by the inhibition of mitochondrial Ca(2+) uptake. ..
  47. Vrbacky M, Krijt J, Drahota Z, Melkova Z. Inhibitory effects of Bcl-2 on mitochondrial respiration. Physiol Res. 2003;52:545-54 pubmed
    ..Based on this observation, we propose that the outcome of Bcl-2 expression is determined by the type of cellular ATP synthesis, namely that Bcl-2 causes apoptosis in cells relying on oxidative phosphorylation. ..
  48. Zhang H, Du G, Zhang J. Assay of mitochondrial functions by resazurin in vitro. Acta Pharmacol Sin. 2004;25:385-9 pubmed
    ..Using resazurin to determine mitochondrial function is sensitive, inexpensive and could be easily automated for high throughput screening. ..
  49. Velthuys B, Amesz J. Charge accumulation at the reducing side of system 2 of photosynthesis. Biochim Biophys Acta. 1974;333:85-94 pubmed
  50. Inui K, Tomita Y, Katsura T, Okano T, Takano M, Hori R. H+ coupled active transport of bestatin via the dipeptide transport system in rabbit intestinal brush-border membranes. J Pharmacol Exp Ther. 1992;260:482-6 pubmed
    ..These results indicate that bestatin is transported via the H+/dipeptide transport system in rabbit intestinal brush-border membranes. ..
  51. Cheng Y, Kelly T, Church J. Potential contribution of a voltage-activated proton conductance to acid extrusion from rat hippocampal neurons. Neuroscience. 2008;151:1084-98 pubmed publisher
    ..Nonetheless, taken together, the results suggest that a Zn2+-sensitive proton conductive pathway is present in rat hippocampal neurons and contributes to H+ efflux under depolarizing conditions. ..
  52. Li X, Mizuno R, Ono N, Ohhashi T. Glucose and glucose transporters regulate lymphatic pump activity through activation of the mitochondrial ATP-sensitive K+ channel. J Physiol Sci. 2008;58:249-61 pubmed publisher
  53. Varela A, Gomes A, Simões A, Teodoro J, Duarte F, Rolo A, et al. Indirubin-3'-oxime impairs mitochondrial oxidative phosphorylation and prevents mitochondrial permeability transition induction. Toxicol Appl Pharmacol. 2008;233:179-85 pubmed publisher
    ..This work shows for the first time multiple effects of indirubin-3'-oxime on mitochondrial bioenergetics thus indicating a potential mechanism for indirubin-3'-oxime effects on cell function. ..
  54. Olsen L, Andersen A, Lunding A, Brasen J, Poulsen A. Regulation of glycolytic oscillations by mitochondrial and plasma membrane H+-ATPases. Biophys J. 2009;96:3850-61 pubmed publisher
    ..We conclude that it is glycolysis that drives the oscillations in mitochondrial membrane potential. ..
  55. Palamarchuk L, Mansurova S, Starkov A. Thyroxine reversibly inhibits the uncoupling action of protonophores on energy production in rat thymus lymphocytes. Biochemistry (Mosc). 2002;67:468-72 pubmed
    ..The data are discussed in terms of a hypothesis that aromatic uncouplers are transported into the cell by the thyroxine carrier of the plasma membrane. ..
  56. Hool L, Di Maria C, Viola H, Arthur P. Role of NAD(P)H oxidase in the regulation of cardiac L-type Ca2+ channel function during acute hypoxia. Cardiovasc Res. 2005;67:624-35 pubmed
  57. Kraunsøe R, Boushel R, Hansen C, Schjerling P, Qvortrup K, Støckel M, et al. Mitochondrial respiration in subcutaneous and visceral adipose tissue from patients with morbid obesity. J Physiol. 2010;588:2023-32 pubmed publisher
    ..Oxidative phosphorylation has a higher relative activity in visceral compared with subcutaneous adipose tissue. ..
  58. Smaili S, Hsu Y, Sanders K, Russell J, Youle R. Bax translocation to mitochondria subsequent to a rapid loss of mitochondrial membrane potential. Cell Death Differ. 2001;8:909-20 pubmed
    ..Taken together, our results suggest that alterations in mitochondrial energization associated with apoptosis can initiate Bax docking to mitochondria. ..
  59. Dufer M, Haspel D, Krippeit Drews P, Kelm M, Ranta F, Nitschke R, et al. The KATP channel is critical for calcium sequestration into non-ER compartments in mouse pancreatic beta cells. Cell Physiol Biochem. 2007;20:65-74 pubmed
    ..The change in Ca(2+) sequestration in beta cells treated with insulinotropic antidiabetics may have implications for beta cell survival and the therapeutic use of these drugs. ..
  60. Kim B, Jeon J, Kim S, So I, Kim K. Regulation of transient receptor potential melastatin 7 (TRPM7) currents by mitochondria. Mol Cells. 2007;23:363-9 pubmed
    ..These results suggest that the inner membrane potential (as regulated by proton ionophores) and the F1/FO-ATP synthase of mitochondria are important in regulating TRPM7 channels. ..
  61. Inoue K, Karashima T, Kamada M, Shuin T, Kurabayashi A, Furihata M, et al. Regulation of 5-aminolevulinic acid-mediated protoporphyrin IX accumulation in human urothelial carcinomas. Pathobiology. 2009;76:303-14 pubmed publisher
    ..This shows that the suppression of ferrochelatase increased PpIX accumulation in UC cells using small amount of ALA, thus leading to an improved clinical practicability of photodynamic diagnosis and therapy. ..
  62. Jornot L, Maechler P, Wollheim C, Junod A. Reactive oxygen metabolites increase mitochondrial calcium in endothelial cells: implication of the Ca2+/Na+ exchanger. J Cell Sci. 1999;112 ( Pt 7):1013-22 pubmed
    ..These results demonstrate that the H2O2-induced elevation in [Ca2+]m results from a transfer of Ca2+ secondary to increased [Ca2+]c, and an inhibition of the Ca2+/Na+ electroneutral exchanger of the mitochondria. ..
  63. Jekabsons M, Horwitz B. Nucleotide effects on liver and muscle mitochondrial non-phosphorylating respiration and membrane potential. Biochim Biophys Acta. 2001;1503:314-28 pubmed
    ..Based on the high concentrations required, CMP is unlikely to be a physiologically important leak regulator. Nonetheless, our results show that tissues other than brown fat have inducible leaks that may be protein-mediated. ..
  64. Soboloff J, Berger S. Sustained ER Ca2+ depletion suppresses protein synthesis and induces activation-enhanced cell death in mast cells. J Biol Chem. 2002;277:13812-20 pubmed
  65. Handa R, Strandhoy J, Giammattei C, Handa S. Platelet-activating factor and solute transport processes in the kidney. Am J Physiol Renal Physiol. 2003;284:F274-81 pubmed
  66. Peers C, Aley P, Boyle J, Porter K, Pearson H, Smith I, et al. Hypoxic regulation of Ca2+ signalling in astrocytes and endothelial cells. Adv Exp Med Biol. 2006;580:185-90; discussion 351-9 pubmed
  67. Cosentino Gomes D, Russo Abrahão T, Fonseca de Souza A, Ferreira C, Galina A, Meyer Fernandes J. Modulation of Trypanosoma rangeli ecto-phosphatase activity by hydrogen peroxide. Free Radic Biol Med. 2009;47:152-8 pubmed publisher
    ..It seems that H(2)O(2) plays a fundamental role in the regulation of cellular processes of these organisms. We showed, for the first time, that these parasites can produce H(2)O(2), and it is able to regulate ecto-phosphatase activity. ..
  68. Barrett M, Tetaud E, Seyfang A, Bringaud F, Baltz T. Functional expression and characterization of the Trypanosoma brucei procyclic glucose transporter, THT2. Biochem J. 1995;312 ( Pt 3):687-91 pubmed
    ..THT2 expressed in CHO cells behaved as a facilitated transporter, and was unaffected by FCCP or KCN over the whole substrate concentration range tested...
  69. Steinert K, Wagner V, Kroth Pancic P, Bickel Sandkötter S. Characterization and subunit structure of the ATP synthase of the halophilic archaeon Haloferax volcanii and organization of the ATP synthase genes. J Biol Chem. 1997;272:6261-9 pubmed
    ..This subunit has been isolated and purified; its molecular mass as deduced by SDS-polyacrylamide gel electrophoresis is 9.7 kDa...
  70. Khodorov B, Fayuk D, Koshelev S, Vergun O, Pinelis V, Vinskaya N, et al. Effect of a prolonged glutamate challenge on plasmalemmal calcium permeability in mammalian central neurones. Mn2+ as a tool to study calcium influx pathways. Int J Neurosci. 1996;88:215-41 pubmed
    ..These findings give new evidence in favour of the hypothesis that the GLU-induced Ca2+ overload of the neuron mainly from an impairment of its Ca2+ extrusion systems. ..
  71. Maechler P, Antinozzi P, Wollheim C. Modulation of glutamate generation in mitochondria affects hormone secretion in INS-1E beta cells. IUBMB Life. 2000;50:27-31 pubmed
    ..These results further point to glutamate as an intracellular messenger playing a key role in the control of insulin exocytosis. ..
  72. Krick S, Platoshyn O, Sweeney M, Kim H, Yuan J. Activation of K+ channels induces apoptosis in vascular smooth muscle cells. Am J Physiol Cell Physiol. 2001;280:C970-9 pubmed
    ..The resultant K+ loss through opened maxi-K channels may serve as a trigger for cell shrinkage and caspase activation, which are major characteristics of apoptosis in pulmonary vascular smooth muscle cells...
  73. Shabalina I, Kramarova T, Nedergaard J, Cannon B. Carboxyatractyloside effects on brown-fat mitochondria imply that the adenine nucleotide translocator isoforms ANT1 and ANT2 may be responsible for basal and fatty-acid-induced uncoupling respectively. Biochem J. 2006;399:405-14 pubmed
    ..The data are therefore compatible with a tentative model in which the ANT2 isoform mediates fatty-acid-induced uncoupling, whereas the ANT1 isoform may mediate a significant part of the high basal proton leak in brown-fat mitochondria. ..
  74. Brennan J, Berry R, Baghai M, Duchen M, Shattock M. FCCP is cardioprotective at concentrations that cause mitochondrial oxidation without detectable depolarisation. Cardiovasc Res. 2006;72:322-30 pubmed
    ..This establishes the principle that mild mitochondrial uncoupling activates a protective mechanism. Diazoxide did not cause mitochondrial oxidation or mitochondrial depolarisation, suggesting it induces protection via another mechanism. ..
  75. Cuchillo Ibanez I, Olivares R, Aldea M, Villarroya M, Arroyo G, Fuentealba J, et al. Acetylcholine and potassium elicit different patterns of exocytosis in chromaffin cells when the intracellular calcium handling is disturbed. Pflugers Arch. 2002;444:133-42 pubmed
    ..This implies that mitochondria are more sensitive to the local [Ca2+](c) changes resulting from the physiological triggering of action potentials by ACh. ..
  76. Kruglov A, Yurkov I, Teplova V, Evtodienko Y. Lucigenin-derived chemiluminescence in intact isolated mitochondria. Biochemistry (Mosc). 2002;67:1262-70 pubmed
    ..Based on our own and literature data, a scheme of LDC generation is suggested. The origin of superoxide anion detected in intact mitochondria with lucigenin is discussed. ..
  77. Vanden Berghe P, Hennig G, Smith T. Characteristics of intermittent mitochondrial transport in guinea pig enteric nerve fibers. Am J Physiol Gastrointest Liver Physiol. 2004;286:G671-82 pubmed
    ..We also show that mitochondrial transport is TTX sensitive and speeds up by stabilizing actin and that functional Ca(2+) stores are required for efficient transport. ..
  78. Basso E, Fante L, Fowlkes J, Petronilli V, Forte M, Bernardi P. Properties of the permeability transition pore in mitochondria devoid of Cyclophilin D. J Biol Chem. 2005;280:18558-61 pubmed
    ..These results have major implications for our current understanding of the PTP and its modulation in vitro and in vivo. ..
  79. Suzuki Y, Yoshimaru T, Inoue T, Ra C. Mitochondrial Ca2+ flux is a critical determinant of the Ca2+ dependence of mast cell degranulation. J Leukoc Biol. 2006;79:508-18 pubmed
    ..This finding may shed light on a new role for mitochondria in the regulation of mast cell activation. ..
  80. Sedova M, Dedkova E, Blatter L. Integration of rapid cytosolic Ca2+ signals by mitochondria in cat ventricular myocytes. Am J Physiol Cell Physiol. 2006;291:C840-50 pubmed
    ..Thus in cardiac myocytes fast [Ca(2+)](i) transients are integrated by mitochondrial Ca(2+) transport systems, resulting in a frequency-dependent net mitochondrial Ca(2+) accumulation. ..
  81. Valmas N, Zuryn S, Ebert P. Mitochondrial uncouplers act synergistically with the fumigant phosphine to disrupt mitochondrial membrane potential and cause cell death. Toxicology. 2008;252:33-9 pubmed publisher
    ..Furthermore, we have identified a novel pathway that can be targeted to overcome genetic resistance to phosphine. ..
  82. Guidot D. Endotoxin pretreatment in vivo increases the mitochondrial respiratory capacity in rat hepatocytes. Arch Biochem Biophys. 1998;354:9-17 pubmed
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