benomyl

Summary

Summary: A systemic agricultural fungicide used for control of certain fungal diseases of stone fruit.

Top Publications

  1. Thrower D, Stemple J, Yeh E, Bloom K. Nuclear oscillations and nuclear filament formation accompany single-strand annealing repair of a dicentric chromosome in Saccharomyces cerevisiae. J Cell Sci. 2003;116:561-9 pubmed
    ..that the frequency of spontaneous direct repeat recombination is suppressed in yeast cells treated with benomyl, a drug that perturbs microtubules...
  2. Machin N, Lee J, Barnes G. Microtubule stability in budding yeast: characterization and dosage suppression of a benomyl-dependent tubulin mutant. Mol Biol Cell. 1995;6:1241-59 pubmed
    ..Overexpression of the JSN1 gene in a TUB2 strain causes that strain to become more sensitive to benomyl, a microtubule-destabilizing drug...
  3. Lebre S, Becq J, Devaux F, Stumpf M, Lelandais G. Statistical inference of the time-varying structure of gene-regulation networks. BMC Syst Biol. 2010;4:130 pubmed publisher
  4. Morinaga H, Yanase T, Nomura M, Okabe T, Goto K, Harada N, et al. A benzimidazole fungicide, benomyl, and its metabolite, carbendazim, induce aromatase activity in a human ovarian granulose-like tumor cell line (KGN). Endocrinology. 2004;145:1860-9 pubmed
    ..Only benomyl, known as both a benzimidazole fungicide and a microtubule-interfering agent, was found to induce aromatase ..
  5. Paul A, Semer C, Kucharek T, Ferl R. The fungicidal and phytotoxic properties of benomyl and PPM in supplemented agar media supporting transgenic arabidopsis plants for a Space Shuttle flight experiment. Appl Microbiol Biotechnol. 2001;55:480-5 pubmed
    ..In a series of experiments designed to optimize media composition for a recent shuttle mission, the fungicide benomyl and the biocide "Plant Preservative Mixture" (PPM) were evaluated for effectiveness in controlling ..
  6. Rathinasamy K, Panda D. Kinetic stabilization of microtubule dynamic instability by benomyl increases the nuclear transport of p53. Biochem Pharmacol. 2008;76:1669-80 pubmed publisher
    ..green fluorescent protein-tubulin transfected MCF-7 cells, we found that a tubulin-targeted antimitotic agent, benomyl at its half-maximal proliferation inhibitory concentration (5 microM) strongly suppressed the rate and extent of ..
  7. Gemeno C, Zurek L, Schal C. Control of Herpomyces spp. (Ascomycetes: Laboulbeniales) infection in the wood cockroach, Parcoblatta lata (Dictyoptera: Blattodea: Blattellidae), with benomyl. J Invertebr Pathol. 2004;85:132-5 pubmed
  8. Yamada T, Sumida K, Saito K, Ueda S, Yabushita S, Sukata T, et al. Functional genomics may allow accurate categorization of the benzimidazole fungicide benomyl: lack of ability to act via steroid-receptor-mediated mechanisms. Toxicol Appl Pharmacol. 2005;205:11-30 pubmed
    Although benomyl and its metabolite carbendazim have been shown to adversely affect male reproduction, the mechanisms of action do not appear to involve the endocrine system...
  9. Rathinasamy K, Panda D. Suppression of microtubule dynamics by benomyl decreases tension across kinetochore pairs and induces apoptosis in cancer cells. FEBS J. 2006;273:4114-28 pubmed
    We found that benomyl, a benzimidazole fungicide, strongly suppressed the reassembly of cold-depolymerized spindle microtubules in HeLa cells...

More Information

Publications62

  1. Amer S, Donya S, Aly F. Genotoxicity of benomyl and its residues in somatic and germ cells of mice fed on treated stored wheat grains. Arch Toxicol. 2003;77:712-21 pubmed
    Swiss mice were fed for 2, 4 and 8 weeks wheat grains treated with 1, 2, and 4 g benomyl/kg and stored for 6 and 12 weeks...
  2. Rischitor P, Konzack S, Fischer R. The Kip3-like kinesin KipB moves along microtubules and determines spindle position during synchronized mitoses in Aspergillus nidulans hyphae. Eukaryot Cell. 2004;3:632-45 pubmed
    ..kipB mutant strains were resistant to high concentrations of the microtubule-destabilizing drug benomyl, suggesting that KipB destabilizes microtubules...
  3. Amorim M, Römbke J, Soares A. Avoidance behaviour of Enchytraeus albidus: effects of benomyl, carbendazim, phenmedipham and different soil types. Chemosphere. 2005;59:501-10 pubmed
    ..In the tests reported here, the enchytraeids were exposed to control soils spiked with the fungicides Benomyl and Carbendazim and the herbicide Phenmedipham...
  4. Hewitt M, Mutch P, Pratten M. Potential teratogenic effects of benomyl in rat embryos cultured in vitro. Reprod Toxicol. 2005;20:271-80 pubmed
    A possible association between environmental exposure to benomyl and anophthalmia has been suggested. The aim of the present study was to investigate potential teratogenic effects of benomyl using the 9...
  5. Fardeau V, Lelandais G, Oldfield A, Salin H, Lemoine S, Garcia M, et al. The central role of PDR1 in the foundation of yeast drug resistance. J Biol Chem. 2007;282:5063-74 pubmed
    ..The activity of Pdr1p in early drug response is not drug-specific, as two chemically unrelated drugs, benomyl and fluphenazine, elicit identical, Pdr1p-dependent, ePDR patterns...
  6. Saunders W, Hornack D, Lengyel V, Deng C. The Saccharomyces cerevisiae kinesin-related motor Kar3p acts at preanaphase spindle poles to limit the number and length of cytoplasmic microtubules. J Cell Biol. 1997;137:417-31 pubmed
    ..Addition of the microtubule polymerization inhibitors nocodazol or benomyl to the medium or deletion of the nonessential alpha-tubulin TUB3 gene can mostly correct the abnormal microtubule ..
  7. Ben Yaacov R, Knoller S, Caldwell G, Becker J, Koltin Y. Candida albicans gene encoding resistance to benomyl and methotrexate is a multidrug resistance gene. Antimicrob Agents Chemother. 1994;38:648-52 pubmed
    ..A gene (BENr) formerly shown to be responsible for the resistance of C. albicans to benomyl and methotrexate was shown in the present study to confer resistance to four other inhibitory compounds: ..
  8. Kucera S, Swann J, Kennedy J, Schultz T. The effects of benomyl and its breakdown products carbendazim and butyl isocyanate on the structure and function of tracheal ciliated cells. J Environ Sci Health B. 1995;30:779-99 pubmed
    The effects of the fungicide benomyl and its breakdown products, carbendazim and butyl isocyanate, were examined on canine tracheal epithelial tissue in primary culture...
  9. Clément M, Rathinasamy K, Adjadj E, Toma F, Curmi P, Panda D. Benomyl and colchicine synergistically inhibit cell proliferation and mitosis: evidence of distinct binding sites for these agents in tubulin. Biochemistry. 2008;47:13016-25 pubmed publisher
    b>Benomyl, a tubulin-targeted antimitotic antifungal agent, belongs to the benzimidazole group of compounds, which are known to inhibit the binding of colchicine to tubulin...
  10. Rognon B, Kozovska Z, Coste A, Pardini G, Sanglard D. Identification of promoter elements responsible for the regulation of MDR1 from Candida albicans, a major facilitator transporter involved in azole resistance. Microbiology. 2006;152:3701-22 pubmed
    ..In an azole-susceptible strain, transcription of this reporter is transiently upregulated in response to either benomyl or H(2)O(2), whereas its expression is constitutively high in an azole-resistant strain (FR2)...
  11. Yoon C, Jin J, Park J, Yeo C, Kim S, Hwang Y, et al. Toxic effects of carbendazim and n-butyl isocyanate, metabolites of the fungicide benomyl, on early development in the African clawed frog, Xenopus laevis. Environ Toxicol. 2008;23:131-44 pubmed publisher
    We investigated the toxic effects of carbendazim and n-butyl isocyanate (BIC), metabolites of the fungicide benomyl, on development in the African clawed frog, Xenopus laevis...
  12. Gupta K, Bishop J, Peck A, Brown J, Wilson L, Panda D. Antimitotic antifungal compound benomyl inhibits brain microtubule polymerization and dynamics and cancer cell proliferation at mitosis, by binding to a novel site in tubulin. Biochemistry. 2004;43:6645-55 pubmed
    The antifungal agent benomyl [methyl-1-(butylcarbamoyl)-2-benzimidazolecarbamate] is used throughout the world against a wide range of agricultural fungal diseases...
  13. Wilson G, Williamson M. Topsin-M: the new benomyl for mycorrhizal-suppression experiments. Mycologia. 2008;100:548-54 pubmed
    The fungicide benomyl was the most commonly used biocide for both field and greenhouse experiments in which arbuscular mycorrhizal fungal (AMF) suppression is desired...
  14. Hoyt M, Totis L, Roberts B. S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function. Cell. 1991;66:507-17 pubmed
    ..Our studies have defined three genes required for normal cell cycle arrest. These findings are consistent with the existence of a surveillance system that halts the cell cycle in response to microtubule perturbation...
  15. Stearns T, Hoyt M, Botstein D. Yeast mutants sensitive to antimicrotubule drugs define three genes that affect microtubule function. Genetics. 1990;124:251-62 pubmed
    ..cerevisiae were isolated by screening for mutants displaying supersensitivity to the antimicrotubule drug benomyl. Such mutants fall into six complementation groups: TUB1, TUB2 and TUB3, the three tubulin genes of yeast, and ..
  16. Chen R, Brady D, Smith D, Murray A, Hardwick K. The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins. Mol Biol Cell. 1999;10:2607-18 pubmed
    ..Deletion and mutational analysis of both proteins indicate that association of Mad2p with Mad1p is critical for checkpoint function and for hyperphosphorylation of Mad1p...
  17. Tenreiro S, Fernandes A, Sá Correia I. Transcriptional activation of FLR1 gene during Saccharomyces cerevisiae adaptation to growth with benomyl: role of Yap1p and Pdr3p. Biochem Biophys Res Commun. 2001;280:216-22 pubmed
    The adaptation of Saccharomyces cerevisiae to growth in the presence of the antimitotic fungicide benomyl involves the dramatic activation of FLR1 transcription, taking place during benomyl-induced latency following sudden exposure to ..
  18. McCarroll N, Protzel A, Ioannou Y, Frank Stack H, Jackson M, Waters M, et al. A survey of EPA/OPP and open literature on selected pesticide chemicals. III. Mutagenicity and carcinogenicity of benomyl and carbendazim. Mutat Res. 2002;512:1-35 pubmed
    The known aneuploidogens, benomyl and its metabolite, carbendazim (methyl 2-benzimidazole carbamate (MBC)), were selected for the third in a series of ongoing projects with selected pesticides...
  19. Geissler S, Siegers K, Schiebel E. A novel protein complex promoting formation of functional alpha- and gamma-tubulin. EMBO J. 1998;17:952-66 pubmed
    ..null mutants are synthetically lethal with tub4-1 and super-sensitive towards the microtubule-depolymerizing drug benomyl. All except Deltagim4 are cold-sensitive and their microtubules disassemble at 14 degrees C...
  20. Nakaune R, Nakano M. Benomyl resistance of Colletotrichum acutatum is caused by enhanced expression of beta-tubulin 1 gene regulated by putative leucine zipper protein CaBEN1. Fungal Genet Biol. 2007;44:1324-35 pubmed
    ..acutatum, the fungus causing anthracnose disease of various fruits and crops, has inherent resistance to benomyl. The mechanism underlying its resistance to benomyl remains unclear...
  21. Ramirez Mares M, de Mejia E. Comparative study of the antioxidant effect of ardisin and epigallocatechin gallate in rat hepatocytes exposed to benomyl and 1-nitropyrene. Food Chem Toxicol. 2003;41:1527-35 pubmed
    ..the antioxidant effect of ardisin and epigallocatechin 3-O-gallate (EGCG) in hepatocytes exposed to either benomyl or 1-nitropyrene (1-NP)...
  22. Cao Y, Peng G, He Z, Wang Z, Yin Y, Xia Y. Transformation of Metarhizium anisopliae with benomyl resistance and green fluorescent protein genes provides a tag for genetically engineered strains. Biotechnol Lett. 2007;29:907-11 pubmed
    A plasmid, pBGFP, carrying green fluorescent protein (gfp) and benomyl-resistance genes was constructed and transformed into Metarhizium anisopliae. The transformants grew normally and GFP fluorescence was detected...
  23. Lelandais G, Tanty V, Geneix C, Etchebest C, Jacq C, Devaux F. Genome adaptation to chemical stress: clues from comparative transcriptomics in Saccharomyces cerevisiae and Candida glabrata. Genome Biol. 2008;9:R164 pubmed publisher
  24. Fling M, Kopf J, Tamarkin A, Gorman J, Smith H, Koltin Y. Analysis of a Candida albicans gene that encodes a novel mechanism for resistance to benomyl and methotrexate. Mol Gen Genet. 1991;227:318-29 pubmed
    The pathogenic yeast, Candida albicans, is insensitive to the anti-mitotic drug, benomyl, and to the dihydrofolate reductase inhibitor, methotrexate...
  25. Horio T, Oakley B. The role of microtubules in rapid hyphal tip growth of Aspergillus nidulans. Mol Biol Cell. 2005;16:918-26 pubmed
    ..and we used a green fluorescent protein-alpha-tubulin fusion to observe the effects of the antimicrotubule agent benomyl. Hyphal tip cells grew approximately 5 times faster than binucleate germlings...
  26. Hess R, Nakai M. Histopathology of the male reproductive system induced by the fungicide benomyl. Histol Histopathol. 2000;15:207-24 pubmed publisher
    b>Benomyl is an effective fungicide that has been in use for many years...
  27. Lucau Danila A, Lelandais G, Kozovska Z, Tanty V, Delaveau T, Devaux F, et al. Early expression of yeast genes affected by chemical stress. Mol Cell Biol. 2005;25:1860-8 pubmed
    ..modifications of the yeast gene expression program which immediately follows the addition of the antimitotic drug benomyl. Similar analyses were conducted with different isogenic yeast strains in which genes coding for relevant ..
  28. Taheri Talesh N, Horio T, Araújo Bazán L, Dou X, Espeso E, Peñalva M, et al. The tip growth apparatus of Aspergillus nidulans. Mol Biol Cell. 2008;19:1439-49 pubmed publisher
    ..Microtubules are important for delivering vesicles to the tip area and for holding the tip growth apparatus in position...
  29. Brôco N, Tenreiro S, Viegas C, Sá Correia I. FLR1 gene (ORF YBR008c) is required for benomyl and methotrexate resistance in Saccharomyces cerevisiae and its benomyl-induced expression is dependent on pdr3 transcriptional regulator. Yeast. 1999;15:1595-608 pubmed
    ..the range of compounds to which FLR1 gene confers resistance to the antimitotic systemic benzimidazole fungicide benomyl and the antitumor agent methotrexate, reinforcing the concept that the FLR1 gene is a multidrug resistance (MDR) ..
  30. O CONNOR P, Manjarrez M, Smith S. The fate and efficacy of benomyl applied to field soils to suppress activity of arbuscular mycorrhizal fungi. Can J Microbiol. 2009;55:901-4 pubmed publisher
    A systematic application of the fungicide benomyl was used to follow up the suppression of arbuscular mycorrhizal (AM) colonization and to determine its fungitoxic activity and persistence at different depths...
  31. Calatayud A, Barreno E. Chlorophyll a fluorescence, antioxidant enzymes and lipid peroxidation in tomato in response to ozone and benomyl. Environ Pollut. 2001;115:283-9 pubmed
    ..However, benomyl has been found to lessen the adverse impact of ozone on plants...
  32. Gonzalez de Mejia E, Ramirez Mares M, Nair M. Topoisomerase I and II enzyme inhibitory aqueous extract of Ardisia compressa and ardisin protect against benomyl oxidation of hepatocytes. J Agric Food Chem. 2002;50:7714-9 pubmed
    ..antioxidant effect of an aqueous extract from dry leaves of AC and a pure component (ardisin) purified from AC on benomyl (Be)-induced cytotoxicity in primary culture rat hepatocytes...
  33. Ghauch A. Degradation of benomyl, picloram, and dicamba in a conical apparatus by zero-valent iron powder. Chemosphere. 2001;43:1109-17 pubmed
    Reduction of some pesticides (benomyl, picloram, and dicamba) was studied in an aerobic batch conical pilot system to investigate the disappearance of these pesticides on contact with iron powder (20 g/l, 325-mesh)...
  34. Abid M, Li Y, Anthony C, De Benedetti A. Translational regulation of ribonucleotide reductase by eukaryotic initiation factor 4E links protein synthesis to the control of DNA replication. J Biol Chem. 1999;274:35991-8 pubmed
    ..synthetic lethality, with extreme sensitivity to hydroxyurea and the microtubule depolymerizing agent, benomyl. The double mutant (cdc33-1 rnr2-68) also identified a unique terminal phenotype, arrested with small bud and a ..
  35. Rocha L, Luz C. Utility of six fungicides for selective isolation of Evlachovaea sp. and Tolypocladium cylindrosporum. Mycopathologia. 2009;167:341-50 pubmed publisher
    The effects of the fungicides dodine, benomyl, thiabendazole, mancozeb, cupric sulfate, and copper oxychloride were examined in vitro upon germination and further development of Evlachovaea sp. and Tolypocladium cylindrosporum...
  36. Borchsenius A, Tchourikova A, Inge Vechtomov S. Recessive mutations in SUP35 and SUP45 genes coding for translation release factors affect chromosome stability in Saccharomyces cerevisiae. Curr Genet. 2000;37:285-91 pubmed
    ..This phenotype was correlated with an increased sensitivity to the microtubule-poisoning drug benomyl which affects chromosome segregation at anaphase...
  37. Ma Z, Yoshimura M, Michailides T. Identification and characterization of benzimidazole resistance in Monilinia fructicola from stone fruit orchards in California. Appl Environ Microbiol. 2003;69:7145-52 pubmed
    Low and high levels of resistance to the benzimidazole fungicides benomyl and thiophanate-methyl were observed in field isolates of Monilinia fructicola, which is the causative agent of brown rot of stone fruit...
  38. Brachat A, Kilmartin J, Wach A, Philippsen P. Saccharomyces cerevisiae cells with defective spindle pole body outer plaques accomplish nuclear migration via half-bridge-organized microtubules. Mol Biol Cell. 1998;9:977-91 pubmed
    ..This is in agreement with previous data, indicating that cytoplasmic microtubules are organized by the half-bridge during mating...
  39. Wright A, Hunter C. Mutations in a beta-tubulin disrupt spindle orientation and microtubule dynamics in the early Caenorhabditis elegans embryo. Mol Biol Cell. 2003;14:4512-25 pubmed
    ..One of the alleles causes microtubules to be cold stable and resistant to the microtubule-depolymerizing drug benomyl, whereas the other causes cell cycle-specific defects in microtubule polymerization...
  40. Stachowiak B. Efficiency of selected mutagens in generating Xanthophyllomyces dendrorhous strains hyperproducing astaxanthin. Pol J Microbiol. 2013;62:67-72 pubmed
    ..The aim of the study was to assess the efficiency of four mutagens: UV radiation, benomyl, ethyl methanesulfonate and ethidium bromide in generating asthaxanthin-hyperproducing strains of the yeast ..
  41. Capkin E, Terzi E, Boran H, Yandi I, Altinok I. Effects of some pesticides on the vital organs of juvenile rainbow trout (Oncorhynchus mykiss). Tissue Cell. 2010;42:376-82 pubmed publisher
    ..different sublethal concentrations of carbosulfan (25, 50 and 200 ?gL(-1)), propineb (3, 6 and 24 mgL(-1)), and benomyl (2, 5 and 20 mgL(-1)) for 14 days...
  42. Zhang J, Tan K, Wu X, Chen G, Sun J, Reck Peterson S, et al. Aspergillus myosin-V supports polarized growth in the absence of microtubule-based transport. PLoS ONE. 2011;6:e28575 pubmed publisher
    ..Finally, our genetic analyses also indicate that KINA (kinesin-1) rather than UNCA (kinesin-3) is the major kinesin motor that supports polarized growth in the absence of MYOV...
  43. Gold S, Bakkeren G, Davies J, Kronstad J. Three selectable markers for transformation of Ustilago maydis. Gene. 1994;142:225-30 pubmed
    ..The third gene encodes an allele of U. maydis beta-tubulin that confers resistance to the fungicide benomyl.
  44. Mazzoni C, Iacchini S, Serafini A, Falcone C. Characterization of a Kluyveromyces lactis mutant with altered regulation of mitochondrial alcohol dehydrogenases. FEMS Yeast Res. 2006;6:421-7 pubmed
    ..the aar900 mutants had a pleiotropic phenotype and showed increased resistance to monovalent cations and benomyl, suggesting that the mutation could also affect genes other than the alcohol dehydrogenase ones...
  45. Romero C, Desai P, DeLillo N, Vancura A. Expression of FLR1 transporter requires phospholipase C and is repressed by Mediator. J Biol Chem. 2006;281:5677-85 pubmed
    ..Deletion of PLC1 results in benomyl sensitivity, alterations in chromatin structure of centromeres, mitotic delay, and a higher frequency of ..
  46. Kornitzer D, Sharf R, Kleinberger T. Adenovirus E4orf4 protein induces PP2A-dependent growth arrest in Saccharomyces cerevisiae and interacts with the anaphase-promoting complex/cyclosome. J Cell Biol. 2001;154:331-44 pubmed
    ..Finally, we show that E4orf4 can induce G2/M arrest in mammalian cells before apoptosis, indicating that E4orf4-induced events in yeast and mammalian cells are highly conserved...
  47. Fumarola L, Urani C, Crosta G. Quantitative kinetics of damage and recovery of cytoskeletal structure by means of image analysis. Toxicol In Vitro. 2005;19:935-41 pubmed
  48. Slager R, Poole J, LeVan T, Sandler D, Alavanja M, Hoppin J. Rhinitis associated with pesticide exposure among commercial pesticide applicators in the Agricultural Health Study. Occup Environ Med. 2009;66:718-24 pubmed publisher
    ..Rhinitis is common, but the risk factors are not well described. To investigate the association between current rhinitis and pesticide use, we used data from 2245 Iowa commercial pesticide applicators in the Agricultural Health Study...
  49. Ogawa K, Yoshida N, Gesnara W, Omumasaba C, Chamuswarng C. Hybridization and breeding of the benomyl resistant mutant, Trichoderma harziantum antagonized to phytopathogenic fungi by protoplast fusion. Biosci Biotechnol Biochem. 2000;64:833-6 pubmed
    ..from heterokaryons of Trichoderma harzianum by protoplast fusion grew on minimal medium containing 100ppm benomyl. This strain inhibited the growth of the phytopathogenic fungus Fusarium oxysporum f. sp...
  50. Javoreková S, Svrceková I, Maková J. Influence of benomyl and prometryn on the soil microbial activities and community structures in pasture grasslands of Slovakia. J Environ Sci Health B. 2010;45:702-9 pubmed publisher
    ..the effects of the herbicide, Gesagard (prometryn active ingredient), and fungicide, Fundazol 50 WP (benomyl active ingredient), on the microbial respiration activity (CO2 production), the numbers of selective microbial ..
  51. Jimenez G, Yucel J, Rowley R, Subramani S. The rad3+ gene of Schizosaccharomyces pombe is involved in multiple checkpoint functions and in DNA repair. Proc Natl Acad Sci U S A. 1992;89:4952-6 pubmed
    ..An S. pombe genomic clone that complements the UV sensitivity of the rad3-136 mutant completely restores the missing checkpoint functions. The rad3+ gene is also likely to play a role in DNA repair...
  52. Pimienta Barrios E, Gonzalez del Castillo Aranda M, Muñoz Urias A, Nobel P. Effects of benomyl and drought on the mycorrhizal development and daily net CO2 uptake of a wild platyopuntia in a rocky semi-arid environment. Ann Bot. 2003;92:239-45 pubmed
    The effects of drought and the fungicide benomyl on a wild platyopuntia, Opuntia robusta Wendl., growing in a rocky semi-arid environment were assessed...
  53. Ovechkina Y, Maddox P, Oakley C, Xiang X, Osmani S, Salmon E, et al. Spindle formation in Aspergillus is coupled to tubulin movement into the nucleus. Mol Biol Cell. 2003;14:2192-200 pubmed
    ..First, we have used benomyl to disassemble microtubules and create a pool of free tubulin that can be readily observed by immunofluorescence...