trypan blue

Summary

Summary: A diazo-naphthalene sulfonate that is widely used as a stain.

Top Publications

  1. Rezai K, Farrokh Siar L, Gasyna E, Ernest J. Trypan blue induces apoptosis in human retinal pigment epithelial cells. Am J Ophthalmol. 2004;138:492-5 pubmed
    To examine whether trypan blue dye induces apoptosis in human retinal pigment epithelium cells.
  2. Hemmerling A, Harrison W, Brown J, Moscicki B, Oziemkowska M, Bukusi E, et al. Trypan blue staining to determine vaginal exposure in two types of plastic vaginal applicators containing two different microbicide formulations. Sex Transm Dis. 2012;39:710-2 pubmed publisher
    ..Reportedly used applicators returned from 3 clinical trials were stained with 1% trypan blue. In a phase 1 study (VivaGel), using gel-filled HTI polypropylene applicators, 1271 (97%) of applicators ..
  3. Awad D, Schrader I, Bartok M, Mohr A, Gabel D. Comparative toxicology of trypan blue, brilliant blue G, and their combination together with polyethylene glycol on human pigment epithelial cells. Invest Ophthalmol Vis Sci. 2011;52:4085-90 pubmed publisher
    To determine the toxicity in ARPE-19 human retinal pigment epithelium cells of trypan blue (TB) at 0.15% and 0.25% concentration, brilliant blue G (BBG) at 0.025% and 0...
  4. Lai C, Chuang L, Wang N, Yeung L, Chen Y, Hwang Y, et al. Trypan blue selective staining using whole blood for internal limiting membrane peeling during macular hole corrective surgery. Cutan Ocul Toxicol. 2009;28:114-8 pubmed publisher
    To prevent trypan blue (TB) retention and its potential toxicity by selectively staining the internal limiting membrane (ILM) with the aid of whole blood.
  5. Correa G, Veranio G, Silva L, Hirata Junior R, Coil J, Scelza M. Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay. J Appl Oral Sci. 2009;17:457-61 pubmed
    ..The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1...
  6. Kumar T, Majumdar A, Das P, Sarafis V, Ghose M. Trypan blue as a fluorochrome for confocal laser scanning microscopy of arbuscular mycorrhizae in three mangroves. Biotech Histochem. 2008;83:153-9 pubmed publisher
    ..Ceriops tagal and Excoecaria agallocha, collected from forests of the Sundarbans of India were stained with trypan blue to observe arbuscular mycorrhizal colonization...
  7. Strober W. Trypan blue exclusion test of cell viability. Curr Protoc Immunol. 2001;Appendix 3:Appendix 3B pubmed publisher
    ..It is based on the principle that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, Eosin, or propidium, whereas dead cells do not...
  8. Nanavaty M, Johar K, Sivasankaran M, Vasavada A, Praveen M, Zetterström C. Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract. J Cataract Refract Surg. 2006;32:1483-8 pubmed
    To assess the effect of anterior capsule staining with trypan blue 0.0125% on the density and viability of the lens epithelial cells (LECs).
  9. Tran S, Puhar A, Ngo Camus M, Ramarao N. Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus. PLoS ONE. 2011;6:e22876 pubmed publisher
    b>Trypan blue is a dye that has been widely used for selective staining of dead tissues or cells...

More Information

Publications68

  1. Tsaousis K, Kopsachilis N, Tsinopoulos I, Dimitrakos S, Kruse F, Welge Luessen U. Time-dependent morphological alterations and viability of cultured human trabecular cells after exposure to Trypan blue. Clin Exp Ophthalmol. 2013;41:484-90 pubmed publisher
    To determine the time-dependent toxicity of Trypan blue at 0.06% concentration in cultured human trabecular meshwork cells.
  2. Shukla D, Kalliath J, Neelakantan N, Naresh K, Ramasamy K. A comparison of brilliant blue G, trypan blue, and indocyanine green dyes to assist internal limiting membrane peeling during macular hole surgery. Retina. 2011;31:2021-5 pubmed publisher
    To compare surgical outcomes with three dyes, brilliant blue G (BBG), trypan blue (TB) and indocyanine green (ICG), used to facilitate internal limiting membrane peeling during macular hole surgery.
  3. Louis K, Siegel A. Cell viability analysis using trypan blue: manual and automated methods. Methods Mol Biol. 2011;740:7-12 pubmed publisher
    One of the traditional methods of cell viability analysis is the use of trypan blue dye exclusion staining. This technique has been the standard methodology used in academic research laboratories and industrial biotechnology plants...
  4. Penha F, Maia M, Eid Farah M, Príncipe A, Freymüller E, Maia A, et al. Effects of subretinal injections of indocyanine green, trypan blue, and glucose in rabbit eyes. Ophthalmology. 2007;114:899-908 pubmed
    To evaluate the effects of subretinal injections of indocyanine green (ICG), trypan blue, glucose (GL), and balanced salt solution (BSS) in rabbits.
  5. Fauque P, Ben Amor A, Joanne C, Agnani G, Bresson J, Roux C. Use of trypan blue staining to assess the quality of ovarian cryopreservation. Fertil Steril. 2007;87:1200-7 pubmed
    To check the efficiency of the cryopreservation procedure by using trypan blue staining of ovarian test fragments to assess the quality of frozen-thawed ovarian tissue.
  6. Lesnik Oberstein S, de Smet M. Use of heavy Trypan blue in macular hole surgery. Eye (Lond). 2010;24:1177-81 pubmed publisher
    ..Appropriate dyes should be safe, provide adequate staining, and be easy to use. Heavy Trypan blue eliminates the need for an air-fluid exchange (AFX) and appears to have little retinal toxicity...
  7. Schumann R, Gandorfer A, Priglinger S, Kampik A, Haritoglou C. Vital dyes for macular surgery: a comparative electron microscopy study of the internal limiting membrane. Retina. 2009;29:669-76 pubmed publisher
    ..To analyze and compare the ultrastructure of the retinal cleavage plane of the internal limiting membrane (ILM) removed during dye-assisted macular surgery...
  8. Maia M, Penha F, Rodrigues E, Principe A, Dib E, Meyer C, et al. Effects of subretinal injection of patent blue and trypan blue in rabbits. Curr Eye Res. 2007;32:309-17 pubmed
    To investigate the histologic and clinical effects of subretinal injection of patent blue (PB) and trypan blue (TB) in rabbits.
  9. Lesnik Oberstein S, Mura M, Tan S, de Smet M. Heavy trypan blue staining of epiretinal membranes: an alternative to infracyanine green. Br J Ophthalmol. 2007;91:955-7 pubmed
    ..b>Trypan blue (TB) stains ERM and ILM weakly, but with less apparent toxicity than other intraocular dyes...
  10. Li K, Tang E, Li P, Wong D. Double peel using triamcinolone acetonide and trypan blue in the management of myopic macular hole with retinal detachment: a case-control study. Clin Exp Ophthalmol. 2010;38:664-8 pubmed publisher
    To evaluate the safety and efficacy of double peel using triamcinolone acetonide (TA) and trypan blue (TB) in removing epiretinal tissues in vitrectomy for myopic macular hole with retinal detachment (MHRD).
  11. Sharma N, Jhanji V, Titiyal J, Amiel H, Vajpayee R. Use of trypan blue dye during conversion of deep anterior lamellar keratoplasty to penetrating keratoplasty. J Cataract Refract Surg. 2008;34:1242-5 pubmed publisher
    We describe a technique that uses trypan blue dye to identify residual recipient corneal stroma and Descemet membrane (DM) during conversion of deep anterior lamellar keratoplasty (DALK) to penetrating keratoplasty (PKP)...
  12. Sunenshine R, Schultz M, Lawrence M, Shin S, Jensen B, Zubairi S, et al. An outbreak of postoperative gram-negative bacterial endophthalmitis associated with contaminated trypan blue ophthalmic solution. Clin Infect Dis. 2009;48:1580-3 pubmed publisher
    We report 6 cases of postsurgical endophthalmitis due to gram-negative bacteria associated with contaminated trypan blue dye from a compounding pharmacy...
  13. Beutel J, Dahmen G, Ziegler A, Hoerauf H. Internal limiting membrane peeling with indocyanine green or trypan blue in macular hole surgery: a randomized trial. Arch Ophthalmol. 2007;125:326-32 pubmed
    ..To report on anatomical and visual outcomes after vitrectomy and internal limiting membrane peeling for idiopathic macular hole repair...
  14. Portes A, Almeida A, Allodi S, Monteiro M, Miguel N. Trypan blue staining for capsulorhexis: ultrastructural effect on lens epithelial cells and capsules. J Cataract Refract Surg. 2010;36:582-7 pubmed publisher
    To evaluate the ultrastructural effect of trypan blue 0.1% staining for capsulorhexis on lens epithelial cells (LECs) and capsules.
  15. Ghosh S, Issa S, El Ghrably I, Stannard K. Subretinal migration of trypan blue during macular hole and epiretinal membrane peel: an observational case series. Is there a safer method?. Eye (Lond). 2010;24:1724-7 pubmed publisher
    To report the inadvertent subretinal migration and effect of trypan blue (TB) during staining of the epiretinal membrane (ERM) for macular pucker, and internal limiting membrane during macular hole (MH) surgery, and to suggest ..
  16. Jackson T, Kwan A, Laidlaw A, Aylward W. Identification of retinal breaks using subretinal trypan blue injection. Ophthalmology. 2007;114:587-90 pubmed
    To describe the use of subretinal trypan blue to identify retinal breaks during vitrectomy for rhegmatogenous retinal detachment (RD).
  17. Brazitikos P, Katsimpris J, Tsironi E, Androudi S. Retinal nerve fiber layer thickness evaluation after trypan blue-assisted macular surgery. Retina. 2010;30:640-7 pubmed publisher
    The purpose of this study was to evaluate retinal nerve fiber layer (RNFL) thickness changes in eyes undergoing trypan blue-assisted macular surgery using optical coherence tomography-3...
  18. Xiao Y, Wang Y, Fu Z, Hong H. Staining the anterior capsule with indocyanine green or trypan blue for capsulorhexis in eyes with white cataract. Int Ophthalmol. 2004;25:273-6 pubmed
    To assess the efficacy and safety of indocyanine green (ICG) 0.5% and trypan blue 0.1% for anterior capsule staining in cases of white cataract.
  19. Ramarao N, Lereclus D. Adhesion and cytotoxicity of Bacillus cereus and Bacillus thuringiensis to epithelial cells are FlhA and PlcR dependent, respectively. Microbes Infect. 2006;8:1483-91 pubmed
    ..Adhesion and cytotoxicity of B. cereus to epithelial cells might explain the diarrhea caused by these pathogens. Our findings provide further insight into the pathogenicity of B. cereus group members...
  20. Lee B, Kim K, Soh K. Visualizing the network of Bonghan ducts in the omentum and peritoneum by using Trypan blue. J Acupunct Meridian Stud. 2009;2:66-70 pubmed publisher
    A visualizing agent, Trypan blue, was found to be preferentially effective for Bonghan ducts (BHDs) and corpuscles compared to blood vessels or adipose tissues...
  21. Grigg J, Jang J, Fung A, Hunyor A, Wilson T. Trypan blue to assess Baerveldt tube patency after repair of its obstruction. J Glaucoma. 2011;20:571-2 pubmed publisher
    ..We report the use of trypan blue dye to show tubal patency directly after the repair of a blocked Baerveldt tube.
  22. Jhanji V, Chan E, Das S, Zhang H, Vajpayee R. Trypan blue dye for anterior segment surgeries. Eye (Lond). 2011;25:1113-20 pubmed publisher
    Use of vital dyes in ophthalmic surgery has gained increased importance in the past few years. Trypan blue (TB) has been a popular choice among anterior segment surgeons mainly due to its safety, ease of availability, and remarkable ..
  23. Morgan H, McNae I, Nowicki M, Zhong W, Michels P, Auld D, et al. The trypanocidal drug suramin and other trypan blue mimetics are inhibitors of pyruvate kinases and bind to the adenosine site. J Biol Chem. 2011;286:31232-40 pubmed publisher
    ..1904) Berlin Klin. Wochenschrift 20, 329-362) described the efficacy of a series of dye molecules including trypan blue and trypan red to eliminate trypanosome infections in mice...
  24. Henrich P, Priglinger S, Haritoglou C, Schumann R, Strauss R, Schneider U, et al. Quantification of contrast recognizability in sequential epiretinal membrane removal and internal limiting membrane peeling in trypan blue-assisted macular surgery. Retina. 2013;33:818-24 pubmed publisher
    To evaluate the selectivity and strength of intraoperative trypan blue staining during removal of epiretinal membranes (ERMs) and the internal limiting membrane.
  25. Vasavada A, Praveen M, Pandita D, Gajjar D, Vasavada V, Vasavada V, et al. Effect of stromal hydration of clear corneal incisions: quantifying ingress of trypan blue into the anterior chamber after phacoemulsification. J Cataract Refract Surg. 2007;33:623-7 pubmed
    To use trypan blue as a quantifiable ingress tracer to determine whether stromal hydration reduces ocular surface fluid ingress at the end of phacoemulsification.
  26. Lee B, Soh K. Visualization of acupuncture meridians in the hypodermis of rat using Trypan blue. J Acupunct Meridian Stud. 2010;3:49-52 pubmed publisher
    ..Recently we found that Trypan blue preferentially stained primo-vessels and primo-nodes on the surfaces of internal organs, not staining other ..
  27. Saeed M, Heimann H. Atrophy of the retinal pigment epithelium following vitrectomy with trypan blue. Int Ophthalmol. 2009;29:239-41 pubmed publisher
    To report a case of possible trypan blue retinotoxicity after macular hole surgery.
  28. Vote B, Russell M, Joondeph B. Trypan blue-assisted vitrectomy. Retina. 2004;24:736-8 pubmed
    To evaluate the efficacy of trypan blue dye for the staining and removal of the internal limiting membrane, epiretinal membranes, and the posterior hyaloid during pars plana vitrectomy...
  29. Mukhopadhyay I, Nazir A, Mahmood K, Saxena D, Das M, Khanna S, et al. Toxicity of argemone oil: effect on hsp70 expression and tissue damage in transgenic Drosophila melanogaster (hsp70-lacZ) Bg9. Cell Biol Toxicol. 2002;18:1-11 pubmed
    ..tissue damage was investigated by studying beta-galactosidase activity, Western blotting and hybridization, and trypan blue staining in the larval tissues of transgenic Drosophila melanogaster (hsp70-lacZ)Bg9...
  30. Bhartiya P, Sharma N, Ray M, Sinha R, Vajpayee R. Trypan blue assisted phacoemulsification in corneal opacities. Br J Ophthalmol. 2002;86:857-9 pubmed
    To evaluate the efficacy of trypan blue for enhancing visualisation during phacoemulsification and foldable lens implantation in cases of cataract with corneal opacities.
  31. Saini J, Jain A, Sukhija J, Gupta P, Saroha V. Anterior and posterior capsulorhexis in pediatric cataract surgery with or without trypan blue dye: randomized prospective clinical study. J Cataract Refract Surg. 2003;29:1733-7 pubmed
    To evaluate the clinical efficacy of trypan blue 0.1% dye (Blurhex) in creating a complete anterior and posterior capsulorhexis during pediatric cataract surgery...
  32. Feron E, Veckeneer M, Parys Van Ginderdeuren R, Van Lommel A, Melles G, Stalmans P. Trypan blue staining of epiretinal membranes in proliferative vitreoretinopathy. Arch Ophthalmol. 2002;120:141-4 pubmed
    To determine whether trypan blue staining facilitates epiretinal membrane (ERM) removal in proliferative vitreoretinopathy.
  33. Kothari K, Jain S, Shah N. Anterior capsular staining with trypan blue for capsulorhexis in mature and hypermature cataracts. A preliminary study. Indian J Ophthalmol. 2001;49:177-80 pubmed
    To study the efficacy and safety of 0.1% Trypan Blue dye to stain the anterior capsule for capsulorhexis in mature and hypermature cataracts...
  34. Perrier M, Sebag M. Trypan blue-assisted peeling of the internal limiting membrane during macular hole surgery. Am J Ophthalmol. 2003;135:903-5 pubmed
    To evaluate the outcome of surgeries for macular holes using trypan blue to facilitate delamination of the internal limiting membrane.
  35. Kwok A, Lai T, Li W, Yew D, Wong V. Trypan blue- and indocyanine green-assisted epiretinal membrane surgery: clinical and histopathological studies. Eye (Lond). 2004;18:882-8 pubmed
    To evaluate the clinical outcome and electron microscopic findings of trypan blue (Tb) and indocyanine green (ICG) assisted epiretinal membrane (ERM) surgery.
  36. Haritoglou C, Eibl K, Schaumberger M, Mueller A, Priglinger S, Alge C, et al. Functional outcome after trypan blue-assisted vitrectomy for macular pucker: a prospective, randomized, comparative trial. Am J Ophthalmol. 2004;138:1-5 pubmed
    To evaluate functional outcome after the intraoperative application of 0.06% trypan blue during vitrectomy for macular pucker.
  37. Chowdhury P, Raj S, Vasavada A. Inadvertent staining of the vitreous with trypan blue. J Cataract Refract Surg. 2004;30:274-6 pubmed
  38. Haritoglou C, Gandorfer A, Schaumberger M, Priglinger S, Mueller A, Gass C, et al. Trypan blue in macular pucker surgery: an evaluation of histology and functional outcome. Retina. 2004;24:582-90 pubmed
    To evaluate possible adverse effects of trypan blue on the ultrastructure of the human retina, to report on functional outcome of macular pucker surgery with and without the use of trypan blue, and to evaluate the ultrastructure of ..
  39. Murinda S, Rashid K, Roberts R. In vitro assessment of the cytotoxicity of nisin, pediocin, and selected colicins on simian virus 40-transfected human colon and Vero monkey kidney cells with trypan blue staining viability assays. J Food Prot. 2003;66:847-53 pubmed
    ..Bacteriocin-treated cells were assessed for viability by trypan blue staining...
  40. Jacob S, Agarwal A, Agarwal A, Agarwal S, Chowdhary S, Chowdhary R, et al. Trypan blue as an adjunct for safe phacoemulsification in eyes with white cataract. J Cataract Refract Surg. 2002;28:1819-25 pubmed
    ..with posterior chamber intraocular lens (PC IOL) implantation in cases of white cataract with the use of trypan blue as an adjunct for performing continuous curvilinear capsulorhexis (CCC) in the absence of a red reflex.
  41. Sharma N, Gupta V, Vajpayee R. Trypan-blue-assisted posterior capsule plaque removal. J Cataract Refract Surg. 2002;28:916-7 pubmed
  42. Chandra R, Bhat K, Jagetia G. Trypan blue exclusion principle in the evaluation of fibroblast attachment in vitro using V79 cells on the conditioned root surface. Quintessence Int. 2005;36:702-6 pubmed
    ..The objective of this study was to assess the efficacy of trypan blue to evaluate fibroblast attachment in an in vitro model using V79 fibroblasts (embryonic Chinese hamster lung ..
  43. Wong V, Lai T, Lee G, Lam P, Lam D. A prospective study on trypan blue capsule staining under air vs under viscoelastic. Eye (Lond). 2006;20:820-5 pubmed
    To compare the safety and effectiveness of trypan blue capsule staining under air vs under viscoelastic.
  44. Marques D, Marques F, Osher R. Three-step technique for staining the anterior lens capsule with indocyanine green or trypan blue. J Cataract Refract Surg. 2004;30:13-6 pubmed
    A 3-step technique for staining the anterior lens capsule was developed to optimize the use of a capsule dye (trypan blue or indocyanine green) during capsulorhexis...
  45. Busetto S, Trevisan E, Patriarca P, Menegazzi R. A single-step, sensitive flow cytofluorometric assay for the simultaneous assessment of membrane-bound and ingested Candida albicans in phagocytosing neutrophils. Cytometry A. 2004;58:201-6 pubmed
    ..In the present report, we describe a simple, sensitive and reliable flow cytofluorometric method that achieves this goal in a Candida albicans-human neutrophils (PMN) system...
  46. Jevprasesphant R, Penny J, Attwood D, D Emanuele A. Transport of dendrimer nanocarriers through epithelial cells via the transcellular route. J Control Release. 2004;97:259-67 pubmed
  47. Laureano J, Coroneo M. Crystalline lens capsule staining with trypan blue. J Cataract Refract Surg. 2004;30:2046-9 pubmed
    A 1-step method for staining the anterior lens capsule with trypan blue is described. The dye is instilled via a paracentesis port at the start of cataract extraction...
  48. Agrawal S, Agrawal J, Agrawal T. Use of trypan blue to confirm the patency of filtering surgery. J Cataract Refract Surg. 2005;31:235-7 pubmed
  49. Lee K, Dean S, Guest S. A comparison of outcomes after indocyanine green and trypan blue assisted internal limiting membrane peeling during macular hole surgery. Br J Ophthalmol. 2005;89:420-4 pubmed
    To compare the anatomical and visual outcomes of macular hole repair surgery using indocyanine green (ICG) or trypan blue (TB) staining of the internal limiting membrane (ILM).
  50. Kodjikian L, Richter T, Halberstadt M, Beby F, Flueckiger F, Boehnke M, et al. Toxic effects of indocyanine green, infracyanine green, and trypan blue on the human retinal pigmented epithelium. Graefes Arch Clin Exp Ophthalmol. 2005;243:917-25 pubmed
    Indocyanine green, infracyanine green, and trypan blue are frequently used as aids to visualize structures removed during vitreoretinal surgery. But they may have toxic effects on the retina...
  51. Gale J, Proulx A, Gonder J, Mao A, Hutnik C. Comparison of the in vitro toxicity of indocyanine green to that of trypan blue in human retinal pigment epithelium cell cultures. Am J Ophthalmol. 2004;138:64-9 pubmed
    To compare the in vitro toxicity of indocyanine green (ICG) to that of trypan blue (TB) in human retinal pigment epithelium cell cultures. The use of ICG and TB in macular hole surgery is discussed.
  52. Jackson T, Hillenkamp J, Knight B, Zhang J, Thomas D, Stanford M, et al. Safety testing of indocyanine green and trypan blue using retinal pigment epithelium and glial cell cultures. Invest Ophthalmol Vis Sci. 2004;45:2778-85 pubmed
    Indocyanine green (ICG) and trypan blue have been advocated as vital stains for use during macular surgery. The safety of these agents was tested using a cell culture model.
  53. Kwok A, Yeung C, Lai T, Chan K, Pang C. Effects of trypan blue on cell viability and gene expression in human retinal pigment epithelial cells. Br J Ophthalmol. 2004;88:1590-4 pubmed
    To evaluate the effects of trypan blue on cell viability and gene expression in human retinal pigment epithelial (RPE) cells.
  54. Narayanan R, Kenney M, Kamjoo S, Trinh T, Seigel G, Resende G, et al. Trypan blue: effect on retinal pigment epithelial and neurosensory retinal cells. Invest Ophthalmol Vis Sci. 2005;46:304-9 pubmed
    To evaluate the toxicity of trypan blue on retinal cells in vitro.
  55. Nuutila J, Lilius E. Flow cytometric quantitative determination of ingestion by phagocytes needs the distinguishing of overlapping populations of binding and ingesting cells. Cytometry A. 2005;65:93-102 pubmed
    ..This report describes an improved flow cytometric method of analysis for kinetic measurement of phagocytosis of fluorescein isothiocyanate (FITC)-labeled zymosan particles by human leukocytes...
  56. Clemmensen I. Interaction of tetranectin with sulphated polysaccharides and trypan blue. Scand J Clin Lab Invest. 1989;49:719-25 pubmed
    ..Its interactions with various sulphated polysaccharides and trypan blue has been investigated by crossed immunoelectrophoresis, immunosorbent assay (ELISA) and gel filtration ..
  57. Uliasz T, Hewett S. A microtiter trypan blue absorbance assay for the quantitative determination of excitotoxic neuronal injury in cell culture. J Neurosci Methods. 2000;100:157-63 pubmed
    An automated method for the determination of neuronal cell death using trypan blue is described. Following various excitotoxic insults, murine mixed cortical cell cultures are stained with trypan blue (0...
  58. Teba F, Mohr A, Eckardt C, Wong D, Kusaka S, Joondeph B, et al. Trypan blue staining in vitreoretinal surgery. Ophthalmology. 2003;110:2409-12 pubmed
    To evaluate the efficacy of trypan blue for staining the internal limiting membrane (ILM) and epiretinal membranes (ERM) in vitreoretinal surgery.
  59. Yetik H, Devranoglu K, Ozkan S. Determining the lowest trypan blue concentration that satisfactorily stains the anterior capsule. J Cataract Refract Surg. 2002;28:988-91 pubmed
    To determine the lowest concentration of trypan blue that will stain the anterior capsule satisfactorily to perform a safe continuous curvilinear capsulorhexis and to describe a staining technique using a dispersive viscoelastic material.