citraconic anhydrides

Summary

Summary: Methylmaleic anhydrides.

Top Publications

  1. Morshedi D, Ebrahim Habibi A, Moosavi Movahedi A, Nemat Gorgani M. Chemical modification of lysine residues in lysozyme may dramatically influence its amyloid fibrillation. Biochim Biophys Acta. 2010;1804:714-22 pubmed publisher
    ..Results are discussed in terms of the importance of the net charge in control of protein-protein interactions leading to aggregate formation and possible specific roles of lysine residues 96 and 97...
  2. Leong A, Haffajee Z. Citraconic anhydride: a new antigen retrieval solution. Pathology. 2010;42:77-81 pubmed publisher
    ..Citrate buffer at pH 6.0 has become widely embraced as the universal fixative but some antibodies remain capricious and yield poor staining results...
  3. Dai W, Sato S, Ishizaki M, Wakamatsu K, Namimatsu S, Sugisaki Y, et al. A new antigen retrieval method using citraconic anhydride for immunoelectron microscopy: localization of surfactant pro-protein C (proSP-C) in the type II alveolar epithelial cells. J Submicrosc Cytol Pathol. 2004;36:219-24 pubmed
    ..The positive proSP-C products also showed lattice-like structures in the alveoli. Thus, the present AR method provides successful pre-embedding immunoelectron microscopy of glutaraldehyde-fixed tissues...
  4. Liu J, Wang T, Huang M, Song H, Weng L, Ji L. Increased thermal and organic solvent tolerance of modified horseradish peroxidase. Protein Eng Des Sel. 2006;19:169-73 pubmed
    ..Circular dichroism studies proved that these modifications changed the conformation of HRP. The improvements of stability are related to side chain reorientations of aromatics upon both modifications...
  5. Batra P. Regeneration of native ovalbumin conformation disrupted by citraconylation. Biochimie. 1991;73:1397-402 pubmed
    ..0 M. These observations indicate that the protein with all the epsilon-amino groups regenerated by acid treatment has the same conformational stability as the native ovalbumin...
  6. Phanomsri E, Namwat N, Tangrassameeprasert R, Charnteeraprawat C, Paupiroj A. Comparative study for the detection of C4d in paraffin-embedded renal allograft biopsies by immunohistochemical techniques. J Med Assoc Thai. 2010;93 Suppl 3:S61-8 pubmed
    ..Peritubular capillary complement C4d deposition is one of the criteria for diagnosis of acute humoral rejection in kidney allografts. Little information is provided for the effective technique to stain C4d protein...
  7. Park H, Kim C, Lee H, Choi J, Lee S, Yun Y, et al. A mesoporous silica nanoparticle with charge-convertible pore walls for efficient intracellular protein delivery. Nanotechnology. 2010;21:225101 pubmed publisher
    ..The MSN developed in this work may serve as efficient intracellular carriers of many cell-impermeable therapeutic proteins...
  8. Lee Y, Ishii T, Cabral H, Kim H, Seo J, Nishiyama N, et al. Charge-conversional polyionic complex micelles-efficient nanocarriers for protein delivery into cytoplasm. Angew Chem Int Ed Engl. 2009;48:5309-12 pubmed publisher
  9. Chen W, Goldfine H, Ananthanarayanan B, Cho W, Roberts M. Listeria monocytogenes phosphatidylinositol-specific phospholipase C: Kinetic activation and homing in on different interfaces. Biochemistry. 2009;48:3578-92 pubmed publisher
    ..monocytogenes infectivity...

More Information

Publications29

  1. Liu J, Wang M. Improvement of activity and stability of chloroperoxidase by chemical modification. BMC Biotechnol. 2007;7:23 pubmed
    ..Enzymes show relative instability in solvents or at elevated temperature and lower activity in organic solvent than in water. These limit the industrial applications of enzymes...
  2. Lee Y, Fukushima S, Bae Y, Hiki S, Ishii T, Kataoka K. A protein nanocarrier from charge-conversion polymer in response to endosomal pH. J Am Chem Soc. 2007;129:5362-3 pubmed
  3. Horwitz J, Huang Q, Ding L. The native oligomeric organization of alpha-crystallin, is it necessary for its chaperone function?. Exp Eye Res. 2004;79:817-21 pubmed
    ..The data suggest that the native oligomeric state of alpha-crystallin may not be essential for its ability to suppress non-specific aggregation...
  4. Kadlík V, Strohalm M, Kodicek M. Citraconylation--a simple method for high protein sequence coverage in MALDI-TOF mass spectrometry. Biochem Biophys Res Commun. 2003;305:1091-3 pubmed
    ..This improvement is caused both by change of trypsin fragmentation pattern and by disturbance of the protein's native tertiary structure...
  5. Batra P. Conformational stability of citraconylated ovalbumin. Int J Biochem. 1991;23:1375-84 pubmed
    ..This destabilization was due to electrostatic repulsions introduced by the entering citraconyl groups...
  6. Rychlik W, Russ M, Rhoads R. Phosphorylation site of eukaryotic initiation factor 4E. J Biol Chem. 1987;262:10434-7 pubmed
    ..The only serine in this peptide is located at position 53 of eIF-4E. Thus, it is concluded that eIF-4E contains a single site of phosphorylation for an endogenous protein kinase, which is Ser-53 in the human eIF-4E sequence...
  7. Gould A, Norton R. Chemical modification of cationic groups in the polypeptide cardiac stimulant anthopleurin-A. Toxicon. 1995;33:187-99 pubmed
    ..The positive inotropic activity of the cyclohexanedione adduct on isolated guinea-pig atria was identical to that of unmodified anthopleurin-A, indicating that the side-chain of Arg-14 is not required for cardiotonic activity...
  8. Knitter R, Liauw M. Ceramic microreactors for heterogeneously catalysed gas-phase reactions. Lab Chip. 2004;4:378-83 pubmed
    ..Apart from the high thermal and chemical resistance, the lack of any blind activity was found to be another advantage of ceramic components...
  9. Maier K, Wagner E. Acid-labile traceless click linker for protein transduction. J Am Chem Soc. 2012;134:10169-73 pubmed publisher
    ..Successful intracellular delivery of bioactive ß-galactosidase as a model enzyme was also demonstrated using the pH-controlled shuttle system...
  10. Son Y, Kim C, Kim Y, Kweon D, Park Y, Seo J. Effects of citraconylation on enzymatic modification of human proinsulin using trypsin and carboxypeptidase B. Biotechnol Prog. 2009;25:1064-70 pubmed publisher
    ..7 to 77.7%. Consequently, citraconylation of lysine residues blocked the unexpected cleavage of human proinsulin by trypsin, minimized the formation of des-threonine insulin and hence increased the production yield of active insulin...
  11. Johnson D, Cushman R. Purification and characterization of four monofluorescein cobra alpha-toxin derivatives. J Biol Chem. 1988;263:2802-7 pubmed
    ..The dissociation rate binding rate constants were less affected and were reduced 0-5-fold...
  12. Illy C, Thielens N, Arlaud G. Chemical characterization and location of ionic interactions involved in the assembly of the C1 complex of human complement. J Protein Chem. 1993;12:771-81 pubmed
    ..Sequence comparison with mannan binding protein, another collagen-like molecule which binds the C1s-C1r-C1r-C1s tetramer, suggests Arg A38, and HyL B32, B65, and C29 of C1q as possible interaction sites...
  13. Wang S, Cai G, Sui S. Intrinsic fluorescence study of the interaction of human apolipoprotein H with phospholipid vesicles. Biochemistry. 1999;38:9477-84 pubmed
    ..The finding that the detachment effect of cations on the blue shift is remarkably different from that on the quenching extent suggests that ApoH is capable of existing in two different conformations when membrane-bound...
  14. Mossavarali S, Hosseinkhani S, Ranjbar B, Miroliaei M. Stepwise modification of lysine residues of glucose oxidase with citraconic anhydride. Int J Biol Macromol. 2006;39:192-6 pubmed
    ..This was supported by comparative study of secondary and tertiary structure...
  15. Al Jamal J. Characterization of different reactive lysines in bovine heart mitochondrial porin. Biol Chem. 2002;383:1967-70 pubmed
    ..These results suggest the presence of critical charged residues in mitochondrial outer membrane porin that may be responsible for both the channel's selectivity and its voltage dependence...
  16. Becker L, McLeod R, Marcovina S, Yao Z, Koschinsky M. Identification of a critical lysine residue in apolipoprotein B-100 that mediates noncovalent interaction with apolipoprotein(a). J Biol Chem. 2001;276:36155-62 pubmed
    ..Taken together, our data indicate that Lys(680) is critical for the noncovalent interaction of apo(a) and apoB-100 that precedes covalent Lp(a) formation...
  17. Martin S, Harding J. Site of carbamoylation of bovine gamma-II-crystallin by potassium [14C]cyanate. Biochem J. 1989;262:909-15 pubmed
    ..It appears that the alpha-amino group of the N-terminal glycine was the main site of carbamoylation. This site forms part of the charge network on the surface of gamma-II-crystallin...
  18. Hosseinkhani S, Ranjbar B, Naderi Manesh H, Nemat Gorgani M. Chemical modification of glucose oxidase: possible formation of molten globule-like intermediate structure. FEBS Lett. 2004;561:213-6 pubmed
  19. Moriguchi K, Mitamura Y, Iwami J, Hasegawa Y, Higuchi N, Murakami Y, et al. Energy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride. Biotech Histochem. 2012;87:485-93 pubmed publisher
    ..The mapping patterns of net nitrogen were restricted to the outermost cell surface...
  20. Zhang X, Zhang K, Haag R. Multi-stage, charge conversional, stimuli-responsive nanogels for therapeutic protein delivery. Biomater Sci. 2015;3:1487-96 pubmed publisher
    ..5 than at pH 7.4. MTT and real-time cell analysis (RTCA) have illustrated that there is more toxicity at pH 6.5 than at pH 7.4. The apoptosis process induced by CC was determined by flow cytometry. ..