bumetanide

Summary

Summary: A sulfamyl diuretic.

Top Publications

  1. Wang D, Kriegstein A. Blocking early GABA depolarization with bumetanide results in permanent alterations in cortical circuits and sensorimotor gating deficits. Cereb Cortex. 2011;21:574-87 pubmed publisher
    ..because it renders GABA activity excitatory through intracellular Cl(-) accumulation, while blocking NKCC1 with bumetanide suppresses seizures. Bumetanide is currently being tested in clinical trials for treatment of neonatal seizures...
  2. Kobayashi Y, Ohbayashi M, Kohyama N, Yamamoto T. Mouse organic anion transporter 2 and 3 (mOAT2/3[Slc22a7/8]) mediates the renal transport of bumetanide. Eur J Pharmacol. 2005;524:44-8 pubmed
    ..The aim of this study was to examine whether mOAT2/3, as well as hOAT2/3, transports the diuretic bumetanide using a Xenopus laevis oocyte expression system...
  3. Dai Y, Tang J, Zhang J. Role of Cl- in cerebral vascular tone and expression of Na+-K+-2Cl- co-transporter after neonatal hypoxia-ischemia. Can J Physiol Pharmacol. 2005;83:767-73 pubmed
    ..was demonstrated by (i) inhibition of Na+-K+-2Cl- co-transporter (NKCC1) to decreased Cl- influx with bumetanide; (ii) a disabled Cl-/HCO3- exchanger with bicarbonate free HEPES solution; (iii) blockade of Cl- channels using ..
  4. Kim E, Lee M. Pharmacokinetics and pharmacodynamics of intravenous bumetanide in mutant Nagase analbuminemic rats: importance of globulin binding for the pharmacodynamic effects. Biopharm Drug Dispos. 2001;22:147-56 pubmed
    ..Based on the furosemide report, the diuretic effects of another loop diuretic, bumetanide, could be expected in analbuminemic rats if plasma protein binding of bumetanide is considerable in the rats...
  5. Shulga A, Magalhães A, Autio H, Plantman S, Di Lieto A, Nykjær A, et al. The loop diuretic bumetanide blocks posttraumatic p75NTR upregulation and rescues injured neurons. J Neurosci. 2012;32:1757-70 pubmed publisher
    ..Based on this mechanism, we propose the use of the diuretic bumetanide to prevent the requirement for BDNF and consequent neuronal death in the injured areas...
  6. Nardou R, Ben Ari Y, Khalilov I. Bumetanide, an NKCC1 antagonist, does not prevent formation of epileptogenic focus but blocks epileptic focus seizures in immature rat hippocampus. J Neurophysiol. 2009;101:2878-88 pubmed publisher
    ..We report that bumetanide, a NKCC1 antagonist, reduces driving force of GABA-mediated currents (DF(GABA)) in neonatal hippocampal neurons ..
  7. Hannaert P, Alvarez Guerra M, Pirot D, Nazaret C, Garay R. Rat NKCC2/NKCC1 cotransporter selectivity for loop diuretic drugs. Naunyn Schmiedebergs Arch Pharmacol. 2002;365:193-9 pubmed
    It is generally assumed that bumetanide possesses some selectivity for the renal Na-K-Cl cotransporter NKCC2, although the results are scarce in the literature and comparisons were done with extra-renal NKCC1 at its basal, almost silent ..
  8. Mares P. Age- and dose-specific anticonvulsant action of bumetanide in immature rats. Physiol Res. 2009;58:927-30 pubmed
    ..It is maintained by cation-chloride cotransporter NKCC1 which is present in immature brain. Bumetanide is a specific inhibitor of this cotransporter...
  9. O Donnell M, Tran L, Lam T, Liu X, Anderson S. Bumetanide inhibition of the blood-brain barrier Na-K-Cl cotransporter reduces edema formation in the rat middle cerebral artery occlusion model of stroke. J Cereb Blood Flow Metab. 2004;24:1046-56 pubmed
    ..577 to 0.637 in cortex and striatum, indicating substantial edema formation. Intravenous bumetanide (7.6-30...

More Information

Publications62

  1. Lauf P, Misri S, Chimote A, Adragna N. Apparent intermediate K conductance channel hyposmotic activation in human lens epithelial cells. Am J Physiol Cell Physiol. 2008;294:C820-32 pubmed publisher
    ..cell K (Kc) by atomic absorption spectrophotometry, and cell water gravimetrically after exposure to ouabain +/- bumetanide (Na-K pump and NKCC inhibitors), and ion channel inhibitors in varying osmolalities with Na, K, or methyl-d-..
  2. Jang I, Jeong H, Akaike N. Contribution of the Na-K-Cl cotransporter on GABA(A) receptor-mediated presynaptic depolarization in excitatory nerve terminals. J Neurosci. 2001;21:5962-72 pubmed
    ..The higher [Cl(-)](i) was generated by bumetanide-sensitive NKCCs and was responsible for the GABA-induced presynaptic depolarization...
  3. Walter S, Shirley D, Folkerd E, Unwin R. Effects of the potassium channel blocker barium on sodium and potassium transport in the rat loop of Henle in vivo. Exp Physiol. 2001;86:469-74 pubmed
    ..When bumetanide, an inhibitor of the Na(+)-K(+)-2Cl(-) co-transporter, was included in the low-Na(+) perfusate, net K(+) ..
  4. Brillault J, Lam T, Rutkowsky J, Foroutan S, O Donnell M. Hypoxia effects on cell volume and ion uptake of cerebral microvascular endothelial cells. Am J Physiol Cell Physiol. 2008;294:C88-96 pubmed
    ..By ion chromatography methods, we also found that a 30-min exposure to 7.5% O(2) caused an increase in bumetanide-sensitive net Na uptake by the cells without increasing cell Na content...
  5. Margineanu D, Klitgaard H. Differential effects of cation-chloride co-transport-blocking diuretics in a rat hippocampal slice model of epilepsy. Epilepsy Res. 2006;69:93-9 pubmed
    ..We explored the effects of ethacrynic acid (ETA), furosemide (FUR) and bumetanide (BUM), prototypical blockers of cation-Cl- co-transport, on the epileptiform field potentials induced in CA3 ..
  6. Chen H, Luo J, Kintner D, Shull G, Sun D. Na(+)-dependent chloride transporter (NKCC1)-null mice exhibit less gray and white matter damage after focal cerebral ischemia. J Cereb Blood Flow Metab. 2005;25:54-66 pubmed
    ..05). In addition, inhibition of NKCC1 by bumetanide resulted in similar protection in NKCC1(+/+) neurons and astrocytes (P<0.05)...
  7. Kim J, Kim W, Kim Y, Lee Y, Kim Y, Shen F, et al. Chronic hyperosmotic stress converts GABAergic inhibition into excitation in vasopressin and oxytocin neurons in the rat. J Neurosci. 2011;31:13312-22 pubmed publisher
    ..increased expression of Na(+)-K(+)-2Cl(-) cotransporter 1 (NKCC1) in MNCs and was blocked by the NKCC inhibitor bumetanide as well as by decreasing NKCC activity through a reduction of extracellular sodium...
  8. Trout L, Townsley M, Bowden A, Ballard S. Disruptive effects of anion secretion inhibitors on airway mucus morphology in isolated perfused pig lung. J Physiol. 2003;549:845-53 pubmed
    ..Maximal inhibitory concentrations of bumetanide and dimethylamiloride, which respectively block Cl- and HCO3- secretion in porcine airways, induced the ..
  9. Cleary R, Sun H, Huynh T, Manning S, Li Y, Rotenberg A, et al. Bumetanide enhances phenobarbital efficacy in a rat model of hypoxic neonatal seizures. PLoS ONE. 2013;8:e57148 pubmed publisher
    ..and here we show in an established model of neonatal hypoxia-induced seizures that the NKCC1 inhibitor, bumetanide, in combination with phenobarbital is significantly more effective than phenobarbital alone...
  10. Reid K, Guo S, Iyer V. Agents which block potassium-chloride cotransport prevent sound-triggered seizures in post-ischemic audiogenic seizure-prone rats. Brain Res. 2000;864:134-7 pubmed
    The purpose of this study was to determine whether the loop diuretics furosemide, bumetanide and ethacrynic acid, which block the KCC1 potassium-chloride transporter in the kidney loop of Henle and the KCC2 potassium-chloride transporter ..
  11. Edwards D, Shah H, Cao W, Gravenstein N, Seubert C, Martynyuk A. Bumetanide alleviates epileptogenic and neurotoxic effects of sevoflurane in neonatal rat brain. Anesthesiology. 2010;112:567-75 pubmed publisher
    ..and impairment in synaptic plasticity-effects that may be diminished by the Na-K-2Cl cotransporter 1 inhibitor, bumetanide. Electroencephalography, activated caspase-3, and hippocampal long-term potentiation were measured in rats ..
  12. Haas B, Cuddapah V, Watkins S, Rohn K, Dy T, Sontheimer H. With-No-Lysine Kinase 3 (WNK3) stimulates glioma invasion by regulating cell volume. Am J Physiol Cell Physiol. 2011;301:C1150-60 pubmed publisher
    ..WNK3 using specific short hairpin RNA constructs completely abolished NKCC1 activity, as measured by the loss of bumetanide-sensitive cell volume regulation...
  13. Evans R, Park K, Turner R, Watson G, Nguyen H, Dennett M, et al. Severe impairment of salivation in Na+/K+/2Cl- cotransporter (NKCC1)-deficient mice. J Biol Chem. 2000;275:26720-6 pubmed
    ..Consistent with defective Cl(-) uptake, a loss of bumetanide-sensitive Cl(-) influx was observed in parotid acinar cells from mice lacking NKCC1...
  14. Plata C, Meade P, Vazquez N, Hebert S, Gamba G. Functional properties of the apical Na+-K+-2Cl- cotransporter isoforms. J Biol Chem. 2002;277:11004-12 pubmed
    The bumetanide-sensitive Na(+):K(+):2Cl(-) cotransporter (BSC1) is the major pathway for salt reabsorption in the apical membrane of the mammalian thick ascending limb of Henle...
  15. Su G, Kintner D, Flagella M, Shull G, Sun D. Astrocytes from Na(+)-K(+)-Cl(-) cotransporter-null mice exhibit absence of swelling and decrease in EAA release. Am J Physiol Cell Physiol. 2002;282:C1147-60 pubmed
    We reported previously that inhibition of Na(+)-K(+)-Cl(-) cotransporter isoform 1 (NKCC1) by bumetanide abolishes high extracellular K(+) concentration ([K(+)](o))-induced swelling and intracellular Cl(-) accumulation in rat cortical ..
  16. Alvarez L, Candia O, Turner H, Polikoff L. Localization of a Na(+)-K(+)-2Cl(-) cotransporter in the rabbit lens. Exp Eye Res. 2001;73:669-80 pubmed
    Earlier work from this laboratory demonstrated a bumetanide-inhibitable K(+) uptake activity in cultured bovine lens epithelial cells, but not at the anterior surfaces of intact bovine lenses isolated in an Ussing-type chamber...
  17. Minlebaev M, Khazipov R. Antiepileptic effects of endogenous beta-hydroxybutyrate in suckling infant rats. Epilepsy Res. 2011;95:100-9 pubmed publisher
    ..Diazepam exerted anticonvulsive action both under physiological ketosis and after blockade of ketogenesis, and bumetanide had no significant anticonvulsive effects in both conditions...
  18. Khirug S, Yamada J, Afzalov R, Voipio J, Khiroug L, Kaila K. GABAergic depolarization of the axon initial segment in cortical principal neurons is caused by the Na-K-2Cl cotransporter NKCC1. J Neurosci. 2008;28:4635-9 pubmed publisher
    ..Data from NKCC1(-/-) and bumetanide-exposed neurons indicated that the depolarizing E(GABA) at the AIS is set by chloride uptake mediated by the Na-..
  19. Sung K, Kirby M, McDonald M, Lovinger D, Delpire E. Abnormal GABAA receptor-mediated currents in dorsal root ganglion neurons isolated from Na-K-2Cl cotransporter null mice. J Neurosci. 2000;20:7531-8 pubmed
    ..GABA responses is caused by Na-K-2Cl cotransport because reduction of external Cl(-) or application of bumetanide induced a decrease in [Cl(-)](i), whereas an increase in external K(+) caused an apparent [Cl(-)](i) ..
  20. Hochman D. The extracellular space and epileptic activity in the adult brain: explaining the antiepileptic effects of furosemide and bumetanide. Epilepsia. 2012;53 Suppl 1:18-25 pubmed publisher
    ..This includes the loop diuretics furosemide and bumetanide, and alterations of the osmolarity of the ECS...
  21. Sipilä S, Schuchmann S, Voipio J, Yamada J, Kaila K. The cation-chloride cotransporter NKCC1 promotes sharp waves in the neonatal rat hippocampus. J Physiol. 2006;573:765-73 pubmed
    ..Here, we show that bumetanide, a selective inhibitor of neuronal Cl- uptake mediated by the Na+-K+-2Cl- cotransporter isoform 1 (NKCC1), ..
  22. Vargas E, Petrou S, Reid C. Genetic and pharmacological modulation of giant depolarizing potentials in the neonatal hippocampus associates with increased seizure susceptibility. J Physiol. 2013;591:57-65 pubmed publisher
    ..To test this idea we blocked NKCC1 with bumetanide in neonatal mice and reduced the number of GDPs to a level similar to that seen in the mutant mice...
  23. Iwamoto L, Fujiwara N, Nakamura K, Wada R. Na-K-2Cl cotransporter inhibition impairs human lung cellular proliferation. Am J Physiol Lung Cell Mol Physiol. 2004;287:L510-4 pubmed
    ..BSMC) showed a significant concentration-dependent decrease in cell counts after 7 days of exposure to both bumetanide (n=5-10) and furosemide (n=6-16) compared with controls...
  24. Somasekharan S, TANIS J, Forbush B. Loop diuretic and ion-binding residues revealed by scanning mutagenesis of transmembrane helix 3 (TM3) of Na-K-Cl cotransporter (NKCC1). J Biol Chem. 2012;287:17308-17 pubmed publisher
    ..by conformational sensitivity of this residue to 2-(trimethylammonium)ethyl methanethiosulfonate, and the bumetanide insensitivity of M382W is consistent with tryptophan blocking entry of bumetanide into the cavity...
  25. Dzhala V, Brumback A, Staley K. Bumetanide enhances phenobarbital efficacy in a neonatal seizure model. Ann Neurol. 2008;63:222-35 pubmed
    ..Blocking the NKCC1 transporter with bumetanide prevents outward Cl- flux and causes a more negative GABA equilibrium potential (E(GABA)) in immature neurons...
  26. Ng T, Konopka E, Hyderi A, Hshieh S, Tsuji Y, Kim B, et al. Comparison of bumetanide- and metolazone-based diuretic regimens to furosemide in acute heart failure. J Cardiovasc Pharmacol Ther. 2013;18:345-53 pubmed publisher
    Limited data exist comparing the efficacy and safety of bumetanide- or metolazone-based diuretic regimens to furosemide in acute heart failure (HF)...
  27. Migliati E, Meurice N, DuBois P, Fang J, Somasekharan S, Beckett E, et al. Inhibition of aquaporin-1 and aquaporin-4 water permeability by a derivative of the loop diuretic bumetanide acting at an internal pore-occluding binding site. Mol Pharmacol. 2009;76:105-12 pubmed publisher
    ..At a high concentration, the loop diuretic bumetanide has been shown to reduce rat brain edema after ischemic stroke by blocking Na(+)-K(+)-2Cl(-) cotransport...
  28. Dzhala V, Talos D, Sdrulla D, Brumback A, Mathews G, Benke T, et al. NKCC1 transporter facilitates seizures in the developing brain. Nat Med. 2005;11:1205-13 pubmed
    ..The Na(+)-K(+)-2Cl(-) cotransporter (NKCC1) facilitates the accumulation of Cl(-) in neurons. The NKCC1 blocker bumetanide shifted E(Cl) negative in immature neurons, suppressed epileptiform activity in hippocampal slices in vitro and ..
  29. Willis E, Clough G, Church M. Investigation into the mechanisms by which nedocromil sodium, frusemide and bumetanide inhibit the histamine-induced itch and flare response in human skin in vivo. Clin Exp Allergy. 2004;34:450-5 pubmed
    ..In asthma, the Na+/K+/2Cl- cotransporter inhibitors, frusemide and bumetanide, have been reported to have many similar actions to nedocromil sodium...
  30. Pond B, Galeffi F, Ahrens R, Schwartz Bloom R. Chloride transport inhibitors influence recovery from oxygen-glucose deprivation-induced cellular injury in adult hippocampus. Neuropharmacology. 2004;47:253-62 pubmed
    ..Furosemide, which inhibits the NKCC-1 and KCC2 cotransporters, and bumetanide, a more specific NKCC-1 inhibitor, enhanced ATP recovery when measured 3 h after OGD...
  31. Hirono C, Nakamoto T, Sugita M, Iwasa Y, Akagawa Y, Shiba Y. Gramicidin-perforated patch analysis on HCO3- secretion through a forskolin-activated anion channel in rat parotid intralobular duct cells. J Membr Biol. 2001;180:11-9 pubmed
  32. Loscher W, Puskarjov M, Kaila K. Cation-chloride cotransporters NKCC1 and KCC2 as potential targets for novel antiepileptic and antiepileptogenic treatments. Neuropharmacology. 2013;69:62-74 pubmed publisher
    ..There is tentative evidence that loop diuretic compounds such as furosemide and bumetanide may have clinically relevant antiepileptic actions, especially when administered in combination with ..
  33. Pressler R, Mangum B. Newly emerging therapies for neonatal seizures. Semin Fetal Neonatal Med. 2013;18:216-23 pubmed publisher
    ..The loop diuretic bumetanide blocks the neuronal NKCC1 co-transporter and is thought specifically to supress seizures in the immature brain...
  34. Lemonnier E, Degrez C, Phelep M, Tyzio R, Josse F, Grandgeorge M, et al. A randomised controlled trial of bumetanide in the treatment of autism in children. Transl Psychiatry. 2012;2:e202 pubmed publisher
    ..study,(1) we have now performed a double-blind clinical trial using the diuretic, chloride-importer antagonist bumetanide that reduces intracellular chloride reinforcing GABAergic inhibition...
  35. Chao P, Hamilton K. Genistein stimulates electrogenic Cl- secretion via phosphodiesterase modulation in the mouse jejunum. Am J Physiol Cell Physiol. 2009;297:C688-98 pubmed publisher
    ..Theophylline stimulated a bumetanide-sensitive I(sc), indicative of Cl(-) secretion, and abolished genistein's stimulatory action on I(sc)...
  36. Hasannejad H, Takeda M, Taki K, Shin H, Babu E, Jutabha P, et al. Interactions of human organic anion transporters with diuretics. J Pharmacol Exp Ther. 2004;308:1021-9 pubmed
    ..including chlorothiazide, cyclothiazide, hydrochlorothiazide, and trichlormethiazide; loop diuretics, including bumetanide, ethacrynic acid, and furosemide; and carbonic anhydrase inhibitors, including acetazolamide and methazolamide...
  37. Brandt C, Nozadze M, Heuchert N, Rattka M, Loscher W. Disease-modifying effects of phenobarbital and the NKCC1 inhibitor bumetanide in the pilocarpine model of temporal lobe epilepsy. J Neurosci. 2010;30:8602-12 pubmed publisher
    ..In the present study, it was evaluated whether prolonged administration of the selective NKCC1 inhibitor, bumetanide, after a pilocarpine-induced SE modifies the development of epilepsy in adult female rats...
  38. Higashiyama K, Takeuchi S, Azuma H, Sawada S, Yamakawa K, Kakigi A, et al. Bumetanide-induced enlargement of the intercellular space in the stria vascularis critically depends on Na+ transport. Hear Res. 2003;186:1-9 pubmed
    ..Loop diuretics such as bumetanide and furosemide cause an acute enlargement of the intrastrial space in association with a decline in the ..
  39. Yan Y, Dempsey R, Sun D. Na+-K+-Cl- cotransporter in rat focal cerebral ischemia. J Cereb Blood Flow Metab. 2001;21:711-21 pubmed
    ..Artificial cerebral spinal fluid (aCSF) or 100 micromol/L bumetanide (a cotransporter inhibitor) in aCSF were continuously microdialyzed through a microdialysis probe into left ..
  40. O Grady S, Jiang X, Maniak P, Birmachu W, Scribner L, Bulbulian B, et al. Cyclic AMP-dependent Cl secretion is regulated by multiple phosphodiesterase subtypes in human colonic epithelial cells. J Membr Biol. 2002;185:137-44 pubmed
    ..blocked by apical treatment with glibenclamide (an inhibitor of the CFTR Cl channel) and by basolateral bumetanide, an inhibitor of Na-K-2Cl cotransport activity...
  41. Starremans P, Kersten F, Knoers N, van den Heuvel L, Bindels R. Mutations in the human Na-K-2Cl cotransporter (NKCC2) identified in Bartter syndrome type I consistently result in nonfunctional transporters. J Am Soc Nephrol. 2003;14:1419-26 pubmed
    ..the functional implications of six homozygous mutations (G193R, A267S, G319R, A508T, del526N, and Y998X) in the bumetanide-sensitive Na-K-2Cl cotransporter (hNKCC2) identified in patients diagnosed with BS type I...
  42. Pacifici G. Clinical pharmacology of the loop diuretics furosemide and bumetanide in neonates and infants. Paediatr Drugs. 2012;14:233-46 pubmed publisher
    The loop diuretics furosemide and bumetanide are used widely for the management of fluid overload in both acute and chronic disease states...
  43. Barmashenko G, Schmidt M, Hoffmann K. Differences between cation-chloride co-transporter functions in the visual cortex of pigmented and albino rats. Eur J Neurosci. 2005;21:1189-95 pubmed
    ..These results are discussed in relation to functional alterations in the albino visual system. ..
  44. Mercado A, Song L, Vazquez N, Mount D, Gamba G. Functional comparison of the K+-Cl- cotransporters KCC1 and KCC4. J Biol Chem. 2000;275:30326-34 pubmed
    ..KCC1 and KCC4 exhibit differential sensitivity to transport inhibitors, such that KCC4 is much less sensitive to bumetanide and furosemide...
  45. Lemonnier E, Ben Ari Y. The diuretic bumetanide decreases autistic behaviour in five infants treated during 3 months with no side effects. Acta Paediatr. 2010;99:1885-8 pubmed publisher
    ..To test the effects of the chloride co-transporter NKCC1 diuretic compound Bumetanide that reduces [Cl(-) ](I) on IAS...
  46. Kahle K, Barnett S, Sassower K, Staley K. Decreased seizure activity in a human neonate treated with bumetanide, an inhibitor of the Na(+)-K(+)-2Cl(-) cotransporter NKCC1. J Child Neurol. 2009;24:572-6 pubmed publisher
    ..The diuretic bumetanide inhibits NKCC1 and silences seizure activity in rodent models of neonatal seizures, but its effect on seizures ..
  47. Wang D, Kriegstein A. GABA regulates excitatory synapse formation in the neocortex via NMDA receptor activation. J Neurosci. 2008;28:5547-58 pubmed publisher
    ..Blocking NKCC1 with the diuretic bumetanide during development leads to similar persistent changes in cortical circuitry in the adult...
  48. Yan Y, Dempsey R, Flemmer A, Forbush B, Sun D. Inhibition of Na(+)-K(+)-Cl(-) cotransporter during focal cerebral ischemia decreases edema and neuronal damage. Brain Res. 2003;961:22-31 pubmed
    ..Artificial cerebral spinal fluid (aCSF) or 100 microM bumetanide in aCSF were continuously microdialyzed into left cortices either 1 h prior to ischemia plus 2-h ischemia, or ..
  49. Koyama R, Tao K, Sasaki T, Ichikawa J, Miyamoto D, Muramatsu R, et al. GABAergic excitation after febrile seizures induces ectopic granule cells and adult epilepsy. Nat Med. 2012;18:1271-8 pubmed publisher
    ..NKCC1 inhibition with bumetanide after febrile seizures rescued the granule cell ectopia, susceptibility to limbic seizures and development of ..
  50. Hamann S, Herrera Pérez J, Bundgaard M, Alvarez Leefmans F, Zeuthen T. Water permeability of Na+-K+-2Cl- cotransporters in mammalian epithelial cells. J Physiol. 2005;568:123-35 pubmed
    ..The transport rate of the cotransporter was determined from the bumetanide-sensitive component of 86Rb+ uptake, and volume changes were derived from quenching of the fluorescent dye ..
  51. Alvarez L, Candia O, Polikoff L. Beta-adrenergic stimulation of Na(+)-K(+)-2Cl(-) cotransport activity in the rabbit lens. Exp Eye Res. 2003;76:61-70 pubmed
    ..cAMP-elicited flux was attributed to the basolateral Na(+)-K(+)-2Cl(-) cotransporter given its preclusion by bumetanide, a highly selective inhibitor of this symport, and the ineffectiveness of ouabain in mitigating the stimulation...
  52. Musselman T, Zhang Z, Masilamani S. Differential regulation of the bumetanide-sensitive cotransporter (NKCC2) by ovarian hormones. Steroids. 2010;75:760-5 pubmed publisher
    ..Localized regulation of NKCC2 by ovarian hormones may have importance in controlling sodium and water balance over the lifetime of women as the milieu of sex hormones varies. ..
  53. Lee J, Marcus D. Endolymphatic sodium homeostasis by Reissner's membrane. Neuroscience. 2003;119:3-8 pubmed
    ..b>Bumetanide, an inhibitor of the Na(+),2Cl(-),K(+)-cotransporter, had no effect on I(sc)...