glyceraldehyde 3 phosphate

Summary

Summary: An aldotriose which is an important intermediate in glycolysis and in tryptophan biosynthesis.

Top Publications

  1. Castilho M, Pavão F, Oliva G, Ladame S, Willson M, Perie J. Evidence for the two phosphate binding sites of an analogue of the thioacyl intermediate for the Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase-catalyzed reaction, from its crystal structure. Biochemistry. 2003;42:7143-51 pubmed
    ..This result gives strong experimental support for the generally accepted flip-flop model of the catalytic mechanism in GAPDH...
  2. Didierjean C, Corbier C, Fatih M, Favier F, Boschi Muller S, Branlant G, et al. Crystal structure of two ternary complexes of phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus with NAD and D-glyceraldehyde 3-phosphate. J Biol Chem. 2003;278:12968-76 pubmed
    ..Arguments are provided that the structures reported here are representative of a productive enzyme.NAD.D-G3P complex in the ground state (Michaelis complex)...
  3. Yun M, Park C, Kim J, Park H. Structural analysis of glyceraldehyde 3-phosphate dehydrogenase from Escherichia coli: direct evidence of substrate binding and cofactor-induced conformational changes. Biochemistry. 2000;39:10702-10 pubmed
    ..This finding suggests that the cofactor NAD(+) may stabilize the transition state oxyanion of the hemiacetal intermediate in support of the flip-flop model for GAP binding...
  4. Guallar V, Jacobson M, McDermott A, Friesner R. Computational modeling of the catalytic reaction in triosephosphate isomerase. J Mol Biol. 2004;337:227-39 pubmed
    ..Our results are consistent with the available experimental data, and provide an initial picture as to why loop opening when GAP is the product has a higher barrier than when DHAP is the product...
  5. Cook W, Senkovich O, Chattopadhyay D. An unexpected phosphate binding site in glyceraldehyde 3-phosphate dehydrogenase: crystal structures of apo, holo and ternary complex of Cryptosporidium parvum enzyme. BMC Struct Biol. 2009;9:9 pubmed publisher
  6. Souza J, Radi R. Glyceraldehyde-3-phosphate dehydrogenase inactivation by peroxynitrite. Arch Biochem Biophys. 1998;360:187-94 pubmed
  7. Jang M, Kang H, Lee S, Chung S, Kang S, Chi S, et al. Glyceraldehyde-3-phosphate, a glycolytic intermediate, plays a key role in controlling cell fate via inhibition of caspase activity. Mol Cells. 2009;28:559-63 pubmed publisher
    ..Based on these results, we suggest that glyceraldehyde-3-phosphate, a key molecule in several central metabolic pathways, functions as a molecule switch between cell survival and apoptosis...
  8. Sterner R, Schmid F. Biochemistry. De novo design of an enzyme. Science. 2004;304:1916-7 pubmed
  9. Breen A, Glenn E, Yeager A, Olson M. Nucleotide diversity among natural populations of a North American poplar (Populus balsamifera, Salicaceae). New Phytol. 2009;182:763-73 pubmed publisher
    ..Our findings have the potential to aid in the design of sampling regimes for conservation and breeding stock and contribute to historical inferences regarding the factors that shaped the genetic diversity of boreal plant species...

More Information

Publications62

  1. Maithal K, Ravindra G, Nagaraj G, Singh S, Balaram H, Balaram P. Subunit interface mutation disrupting an aromatic cluster in Plasmodium falciparum triosephosphate isomerase: effect on dimer stability. Protein Eng. 2002;15:575-84 pubmed
  2. Pegan S, Rukseree K, Franzblau S, Mesecar A. Structural basis for catalysis of a tetrameric class IIa fructose 1,6-bisphosphate aldolase from Mycobacterium tuberculosis. J Mol Biol. 2009;386:1038-53 pubmed publisher
    ..These concise new details offer a better understanding of the reaction mechanisms for FBAs in general and provide a structural basis for inhibitor design efforts aimed at this class of enzymes...
  3. Rozovsky S, McDermott A. Substrate product equilibrium on a reversible enzyme, triosephosphate isomerase. Proc Natl Acad Sci U S A. 2007;104:2080-5 pubmed
  4. Brunner N, Brinkmann H, Siebers B, Hensel R. NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase from Thermoproteus tenax. The first identified archaeal member of the aldehyde dehydrogenase superfamily is a glycolytic enzyme with unusual regulatory properties. J Biol Chem. 1998;273:6149-56 pubmed
    ..tenax. Its regulatory properties substitute for those lacking in the reversible nonregulated pyrophosphate-dependent phosphofructokinase in this variant of the Embden-Meyerhof-Parnas pathway...
  5. Hemmler R, Becker T, Schleiff E, Bölter B, Stahl T, Soll J, et al. Molecular properties of Oep21, an ATP-regulated anion-selective solute channel from the outer chloroplast membrane. J Biol Chem. 2006;281:12020-9 pubmed
    ..Deletion of a C-terminal putative FX4K binding motif in Oep21 decreased the capability of the channel to tune its ion selectivity by about 50%, whereas current block remained unchanged...
  6. Anastasi C, Crowe M, Sutherland J. Two-step potentially prebiotic synthesis of alpha-d-cytidine-5'-phosphate from d-glyceraldehyde-3-phosphate. J Am Chem Soc. 2007;129:24-5 pubmed
  7. Gotz R, Schlüter E, Shoham G, Zimmermann F. A potential role of the cytoskeleton of Saccharomyces cerevisiae in a functional organization of glycolytic enzymes. Yeast. 1999;15:1619-29 pubmed
    ..These observations can be interpreted to mean that metabolic reorganization from a gluconeogenic to a glycolytic metabolism is facilitated by an intact cytoskeleton in yeast...
  8. Kish S, Lopes Cendes I, Guttman M, Furukawa Y, Pandolfo M, Rouleau G, et al. Brain glyceraldehyde-3-phosphate dehydrogenase activity in human trinucleotide repeat disorders. Arch Neurol. 1998;55:1299-304 pubmed
  9. Vojinovic V, von Stockar U. Influence of uncertainties in pH, pMg, activity coefficients, metabolite concentrations, and other factors on the analysis of the thermodynamic feasibility of metabolic pathways. Biotechnol Bioeng. 2009;103:780-95 pubmed publisher
    ..It also suggests an urgent need for experimental determinations of such factors as a prerequisite for sensible thermodynamic analysis of metabolism on a genome-wide scale...
  10. Sevostyanova I, Solovjeva O, Kochetov G. Two methods for determination of transketolase activity. Biochemistry (Mosc). 2006;71:560-2 pubmed
    ..It is not needed in the second method. The range of transketolase concentration in the activity assay is 0.036-0.144 U/ml for the first method and 1.8-6.8 U/ml for the second one...
  11. de Bari L, Atlante A, Valenti D, Passarella S. Partial reconstruction of in vitro gluconeogenesis arising from mitochondrial l-lactate uptake/metabolism and oxaloacetate export via novel L-lactate translocators. Biochem J. 2004;380:231-42 pubmed
    ..The role of lactate/mitochondria interactions in gluconeogenesis is discussed...
  12. Antikainen J, Kuparinen V, Kupannen V, Lähteenmäki K, Korhonen T. pH-dependent association of enolase and glyceraldehyde-3-phosphate dehydrogenase of Lactobacillus crispatus with the cell wall and lipoteichoic acids. J Bacteriol. 2007;189:4539-43 pubmed
    ..These proteins bound to lipoteichoic acids at a pH below their isoelectric point. The results indicate that lactobacilli rapidly modify their surface properties in response to changes in pH...
  13. Taniguchi S, Asano N, Tomino F, Miwa I. Potentiation of glucose-induced insulin secretion by fagomine, a pseudo-sugar isolated from mulberry leaves. Horm Metab Res. 1998;30:679-83 pubmed
    ..Our findings, together with these considerations, suggest that fagomine potentiates glucose-induced insulin secretion through acceleration of some step(s) after the formation of glyceraldehyde 3-phosphate in the glycolytic pathway...
  14. van der Oost J, Schut G, Kengen S, Hagen W, Thomm M, de Vos W. The ferredoxin-dependent conversion of glyceraldehyde-3-phosphate in the hyperthermophilic archaeon Pyrococcus furiosus represents a novel site of glycolytic regulation. J Biol Chem. 1998;273:28149-54 pubmed
    ..It is discussed that this unprecedented unidirectional reaction couple in the pyrococcal glycolysis and gluconeogenesis gives rise to a novel site of glycolytic regulation that might be widespread among Archaea...
  15. Verho R, Richard P, Jonson P, Sundqvist L, Londesborough J, Penttila M. Identification of the first fungal NADP-GAPDH from Kluyveromyces lactis. Biochemistry. 2002;41:13833-8 pubmed
    ..The presence of an asparagine (Asn46 in NADP-GAPDH) instead of the conserved aspartate found in related but NAD-specific enzymes may explain the ability of NADP-GAPDH to work with NADP as well as NAD...
  16. Koo B, Gong Y, Kim S, Kim C, Lee H. Improvement of coenzyme Q(10) production by increasing the NADH/NAD(+) ratio in Agrobacterium tumefaciens. Biosci Biotechnol Biochem. 2010;74:895-8 pubmed
    ..16 to 3.63 mg/g DCW. Due to the addition of hydroxybutyrate to the culture media, the intracellular NADH/NAD(+) ratio in A603-35-gapA shifted from 1.2 to 1.4, which led to an increase CoQ(10) content (5.27 mg/g DCW)...
  17. Batthyany C, Schopfer F, Baker P, Duran R, Baker L, Huang Y, et al. Reversible post-translational modification of proteins by nitrated fatty acids in vivo. J Biol Chem. 2006;281:20450-63 pubmed
  18. Wall D. Origin and rapid diversification of a tropical moss. Evolution. 2005;59:1413-24 pubmed
    ..Identifying the cause for this rapid diversification requires further testing, but it appears to be related to a general shift in reproductive strategy from sexual to asexual, which may be a key innovation for this young group...
  19. Samland A, Wang M, Sprenger G. MJ0400 from Methanocaldococcus jannaschii exhibits fructose-1,6-bisphosphate aldolase activity. FEMS Microbiol Lett. 2008;281:36-41 pubmed publisher
    ..jannaschii. Previously, MJ0400 was shown to act as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase. This indicates that MJ0400 is involved in both the carbon metabolism and the shikimate pathway in M. jannaschii...
  20. Rohdich F, Kis K, Bacher A, Eisenreich W. The non-mevalonate pathway of isoprenoids: genes, enzymes and intermediates. Curr Opin Chem Biol. 2001;5:535-40 pubmed
    ..The genes specifying these enzymes have been cloned from bacteria, plants and protozoa. Their expression in recombinant bacterial hosts has opened the way to the identification of several novel pathway intermediates...
  21. Hagedoorn P, Freije J, Hagen W. Pyrococcus furiosus glyceraldehyde 3-phosphate oxidoreductase has comparable W(6+/5+) and W(5+/4+) reduction potentials and unusual [4Fe-4S] EPR properties. FEBS Lett. 1999;462:66-70 pubmed
    ..0. The titration results combined with catalytic voltammetry data are consistent with electron transfer from glyceraldehyde 3-phosphate first to the tungsten center, then to the cubane cluster and finally to the ferredoxin...
  22. Pawlak A, Toussaint C, Levy I, Bulle F, Poyard M, Barouki R, et al. Characterization of a large population of mRNAs from human testis. Genomics. 1995;26:151-8 pubmed
    ..This argues for the value of the combination of random sequencing and analysis of cellular expression for large-scale characterization of gene expression in the testis...
  23. Shiozawa M, Fukutani Y, Arai N, Cairns N, Mizutani T, Isaki K, et al. Glyceraldehyde 3-phosphate dehydrogenase and endothelin-1 immunoreactivity is associated with cerebral white matter damage in dentatorubral-pallidoluysian atrophy. Neuropathology. 2003;23:36-43 pubmed
  24. Capa Robles W, Paniagua Michel J, Soto J. The biosynthesis and accumulation of beta-carotene in Dunaliella salina proceed via the glyceraldehyde 3-phosphate/pyruvate pathway. Nat Prod Res. 2009;23:1021-8 pubmed publisher
    ..Consequently, this pathway promotes carotenogenesis and the biosynthesis of C(40) beta-carotene in D. salina...
  25. Morland C, Henjum S, Iversen E, Skrede K, Hassel B. Evidence for a higher glycolytic than oxidative metabolic activity in white matter of rat brain. Neurochem Int. 2007;50:703-9 pubmed
    ..However, the white matter glycogen synthase level was only 20-40% of that in cortex, suggesting that not all glucose-1-phosphate is destined for glycogen formation...
  26. Amyes T, Richard J. Enzymatic catalysis of proton transfer at carbon: activation of triosephosphate isomerase by phosphite dianion. Biochemistry. 2007;46:5841-54 pubmed
  27. Usui T, Shimohira K, Watanabe H, Hayase F. Detection and determination of glyceraldehyde-derived pyridinium-type advanced glycation end product in streptozotocin-induced diabetic rats. Biosci Biotechnol Biochem. 2007;71:442-8 pubmed
    ..GLAP might be a biomarker for reduced activity of the glyceraldehyde-related enzymes in the metabolic diseases such as diabetic complications...
  28. Ngo H, Harris R, Kimmich N, Casino P, Niks D, Blumenstein L, et al. Synthesis and characterization of allosteric probes of substrate channeling in the tryptophan synthase bienzyme complex. Biochemistry. 2007;46:7713-27 pubmed publisher
    ..3) ASL binding stabilizes the closed conformations of the beta-subunit for the alpha-aminoacrylate and quinonoid forms of the enzyme. (4) The new ASLs exhibit allosteric properties that parallel the behaviors of IGP and G3P...
  29. Toivari M, Ruohonen L, Richard P, Penttila M, Wiebe M. Saccharomyces cerevisiae engineered to produce D-xylonate. Appl Microbiol Biotechnol. 2010;88:751-60 pubmed publisher
    ..Although each of these enzymes enhanced NADPH consumption on D-glucose, they did not enhance D-xylonate production, suggesting that NADP+ was not the main limitation in the current D-xylonate producing strains...
  30. Roy S, Winton M, Black M, Trojanowski J, Lee V. Cytoskeletal requirements in axonal transport of slow component-b. J Neurosci. 2008;28:5248-56 pubmed publisher
    ..These findings do not support the microfilament-complex model, but instead indicate that the transport of protein complexes in SCb is powered by microtubule motors...
  31. Montiel T, Quiroz Baez R, Massieu L, Arias C. Role of oxidative stress on beta-amyloid neurotoxicity elicited during impairment of energy metabolism in the hippocampus: protection by antioxidants. Exp Neurol. 2006;200:496-508 pubmed
    ..Damage and LPO are efficiently prevented by vitamin E. The present results suggest an interaction between oxidative stress and metabolic impairment in the Abeta neurotoxic cascade...
  32. Brosnan J. Metabolic design principles: chemical and physical determinants of cell chemistry. Adv Enzyme Regul. 2005;45:27-36 pubmed
  33. Raschle T, Speziga D, Kress W, Moccand C, Gehrig P, Amrhein N, et al. Intersubunit cross-talk in pyridoxal 5'-phosphate synthase, coordinated by the C terminus of the synthase subunit. J Biol Chem. 2009;284:7706-18 pubmed publisher
    ..We show that ribose 5-phosphate binding triggers strong cooperativity in Pdx1, and the affinity for this substrate is substantially enhanced upon interaction with the Michaelis complex of Pdx2 and glutamine...
  34. Tiessen A, Hendriks J, Stitt M, Branscheid A, Gibon Y, Farré E, et al. Starch synthesis in potato tubers is regulated by post-translational redox modification of ADP-glucose pyrophosphorylase: a novel regulatory mechanism linking starch synthesis to the sucrose supply. Plant Cell. 2002;14:2191-213 pubmed
    ..Furthermore, activation of AGPase resulted in a stimulation of starch synthesis and decreased levels of glycolytic intermediates...
  35. Solov eva O, Bykova I, Meshalkina L, Kovina M, Kochetov G. Cleaving of ketosubstrates by transketolase and the nature of the products formed. Biochemistry (Mosc). 2001;66:932-6 pubmed
    ..However, the glycolaldehyde cannot be found the free state because it condenses with another glycolaldehyde residue formed in the course of the cleavage of another ketosubstrate molecule yielding erythrulose...
  36. Silva T, Oliveira T, Sakai V, Dionísio T, Santos C, Bagnato V, et al. In vivo effects on the expression of vascular endothelial growth factor-A165 messenger ribonucleic acid of an infrared diode laser associated or not with a visible red diode laser. Photomed Laser Surg. 2010;28:63-8 pubmed publisher
  37. Spector A, Wang R, Ma W, Kleiman N. Development and characterization of an H2O2-resistant immortal lens epithelial cell line. Invest Ophthalmol Vis Sci. 2000;41:832-43 pubmed
    ..The manner in which critical biochemical parameters were affected in both conditioned and normal cells is also reported...
  38. Kohlhardt M, Fichtner H, Fröbe U. Metabolites of the glycolytic pathway modulate the activity of single cardiac Na+ channels. FASEB J. 1989;3:1963-7 pubmed
    ..It is tempting to assume that interference with Na+ inactivation is the mode of action underlying the increase in single-channel activity...
  39. Shaeri J, Wright I, Rathbone E, Wohlgemuth R, Woodley J. Characterization of enzymatic D-xylulose 5-phosphate synthesis. Biotechnol Bioeng. 2008;101:761-7 pubmed publisher
    ..The data presented here allow a comparison of both routes for the synthesis, based on experimental information on reaction kinetics. Operational guidelines are proposed which should assist in the scale-up of such syntheses...
  40. Wierenga R, Kapetaniou E, Venkatesan R. Triosephosphate isomerase: a highly evolved biocatalyst. Cell Mol Life Sci. 2010;67:3961-82 pubmed publisher
    ..Two proton shuttling mechanisms, the classical and the criss-cross mechanism, are responsible for the interconversion of the substrates of this enolising enzyme...
  41. Mäkinen P, Koponen J, Kärkkäinen A, Malm T, Pulkkinen K, Koistinaho J, et al. Stable RNA interference: comparison of U6 and H1 promoters in endothelial cells and in mouse brain. J Gene Med. 2006;8:433-41 pubmed
    ..The two most commonly used promoters to drive the short hairpin RNA (shRNA) expression are the human U6 small nuclear promoter (U6) and the human H1 promoter (H1)...
  42. Weber J, Kayser A, Rinas U. Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. II. Dynamic response to famine and feast, activation of the methylglyoxal pathway and oscillatory behaviour. Microbiology. 2005;151:707-16 pubmed
    ..5 mg l(-1) in the medium...
  43. O Donoghue A, Amyes T, Richard J. Hydron transfer catalyzed by triosephosphate isomerase. Products of isomerization of dihydroxyacetone phosphate in D2O. Biochemistry. 2005;44:2622-31 pubmed
  44. König V, Pfeil A, Braus G, Schneider T. Substrate and metal complexes of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae provide new insights into the catalytic mechanism. J Mol Biol. 2004;337:675-90 pubmed
  45. Karlsson H, Guthenberg C, von Döbeln U, Kristenssson K. Extraction of RNA from dried blood on filter papers after long-term storage. Clin Chem. 2003;49:979-81 pubmed
  46. Marchal S, Cobessi D, Rahuel Clermont S, Tete Favier F, Aubry A, Branlant G. Chemical mechanism and substrate binding sites of NADP-dependent aldehyde dehydrogenase from Streptococcus mutans. Chem Biol Interact. 2001;130-132:15-28 pubmed
    ..These results will be presented and discussed from the point of view of the evolution of the catalytic mechanisms of ALDH...
  47. Ishida K, Zhu B, Maeda H. Novel approach to quantitative reverse transcription PCR assay of mRNA component in autopsy material using the TaqMan fluorogenic detection system: dynamics of pulmonary surfactant apoprotein A. Forensic Sci Int. 2000;113:127-31 pubmed
    ..Elevated SP-A expressions were determined in some cases. This system without post-PCR sample handling would be a very useful tool in pathological diagnosis and DNA analysis...
  48. Ukeda H, Hasegawa Y, Ishi T, Sawamura M. Inactivation of Cu,Zn-superoxide dismutase by intermediates of Maillard reaction and glycolytic pathway and some sugars. Biosci Biotechnol Biochem. 1997;61:2039-42 pubmed
    ..SDS-PAGE band patterns indicated that the inactivation by those carbonyl compounds occurred by both crosslinking and site-specific fragmentation of SOD...
  49. Poliquin K, Ershov Y, Cunningham F, Woreta T, Gantt R, Gantt E. Inactivation of sll1556 in Synechocystis strain PCC 6803 impairs isoprenoid biosynthesis from pentose phosphate cycle substrates in vitro. J Bacteriol. 2004;186:4685-93 pubmed
    ..Our findings are consistent with an alternate metabolic shunt for biosynthesis of isoprenoids...
  50. Eubanks L, Poulter C. Rhodobacter capsulatus 1-deoxy-D-xylulose 5-phosphate synthase: steady-state kinetics and substrate binding. Biochemistry. 2003;42:1140-9 pubmed
    ..These results are consistent with an ordered mechanism for DXP synthase where pyruvate binds before GAP/d-glyceraldehyde...
  51. Roy A, Bera S, Patra S, Ray S, Ray M. A novel D-glyceraldehyde-3-phosphate binding protein, a truncated albumin, with D-glyceraldehyde-3-phosphate dehydrogenase inhibitory property. IUBMB Life. 2009;61:995-1000 pubmed publisher
    ..The implication of this protein in the control of glycolysis has been discussed...
  52. Jones A. Triosephosphate metabolism by mature boar spermatozoa. Reprod Fertil Dev. 1997;9:577-81 pubmed
    ..This suggests that these cells may have the capacity to convert glycolytic intermediates into a storage metabolite to conserve carbon atoms for the eventual synthesis of lactate...
  53. Blacklow S, Knowles J. How can a catalytic lesion be offset? The energetics of two pseudorevertant triosephosphate isomerases. Biochemistry. 1990;29:4099-108 pubmed
    ..The results thus provide the beginnings of a detailed understanding of the kinetic refinement of enzyme catalysts...