krebs 2 carcinoma

Summary

Summary: A transplantable neoplasm of mice.

Top Publications

  1. Hilton D, Nicola N, Gough N, Metcalf D. Resolution and purification of three distinct factors produced by Krebs ascites cells which have differentiation-inducing activity on murine myeloid leukemic cell lines. J Biol Chem. 1988;263:9238-43 pubmed
    ..LIF-A had no colony-stimulating activity and induced differentiation in and inhibited the proliferation of only M1 cells. Each factor bound to a unique cell surface receptor with no evidence of direct cross-reactivity...
  2. Miroshnichenko N, Karpova O, Morozov SYu -, Rodionova N, Atabekov J. Translation arrest of potato virus X RNA in Krebs-2 cell-free system: RNase H cleavage promoted by complementary oligodeoxynucleotides. FEBS Lett. 1988;234:65-8 pubmed
    ..No similar translational arrest was found in the rabbit reticulocyte lysate cell-free system...
  3. Omer A, Janas M, Novina C. The chicken or the egg: microRNA-mediated regulation of mRNA translation or mRNA stability. Mol Cell. 2009;35:739-40 pubmed publisher
  4. Svitkin Y, Sonenberg N. A highly efficient and robust in vitro translation system for expression of picornavirus and hepatitis C virus RNA genomes. Methods Enzymol. 2007;429:53-82 pubmed
    ..With further optimization of cell-free conditions, the entire reconstitution of infectious HCV synthesis in vitro might become feasible...
  5. Svitkin Y, Sonenberg N. Cell-free synthesis of encephalomyocarditis virus. J Virol. 2003;77:6551-5 pubmed
    ..Excess input RNA impaired RNA replication and virus production but not translation. This suggests the existence of a negative-feedback mechanism for regulation of RNA replication by the viral plus-strand RNA or proteins...
  6. Tronchere H, Terce F, Record M, Chap H. Phorbol myristate acetate stimulates [3H]choline incorporation into phosphatidylcholine independently of the 'de novo' pathway in Krebs-II ascitic cells: a unique effect of phorbol ester on choline uptake. Biochem J. 1993;293 ( Pt 3):739-44 pubmed
    ..Our data provide evidence that the enhancement of [3H]choline incorporation into PtdCho triggered by PMA and oleic acid proceeds via completely distinct mechanism(s)...
  7. Pryme I, Pusztai A, Bardocz S. A diet containing the lectin phytohaemagglutinin (PHA) slows down the proliferation of Krebs II cell tumours in mice. Cancer Lett. 1994;76:133-7 pubmed
    ..Mice injected s.c. also developed tumours at later times when fed on the PHA diet. A quantitative of ribosomes in polysome-containing fractions showed no major differences in protein synthesis in control mice and those fed the PHA diet...
  8. Pryme I, Bardocz S, Grant G, Duguid T, Brown D, Pusztai A. Switching between control and phytohaemagglutinin-containing diets affects growth of Krebs II ascites cells and produces differences in the levels of putrescine, spermidine and spermine. Cancer Lett. 1995;93:233-7 pubmed
  9. Simpson R, Hilton D, Nice E, Rubira M, Metcalf D, Gearing D, et al. Structural characterization of a murine myeloid leukaemia inhibitory factor. Eur J Biochem. 1988;175:541-7 pubmed
    ..The protein sequence data reported here indicate three sites of post-translational modification (N-linked glycosylation)...

More Information

Publications34

  1. Gough N, Hilton D, Gearing D, Willson T, King J, Nicola N, et al. Biochemical characterization of murine leukaemia inhibitory factor produced by Krebs ascites and by yeast cells. Blood Cells. 1988;14:431-42 pubmed
    ..Moreover, yeast-derived recombinant LIF appears to display a different pattern of glycosylation to both forms of native LIF. From in vitro experiments, we conclude that the nature of the glycosylation is not crucial to biologic activity...
  2. Hilton D, Nicola N, Metcalf D. Purification of a murine leukemia inhibitory factor from Krebs ascites cells. Anal Biochem. 1988;173:359-67 pubmed
    ..Sufficient quantities of the factor (12 micrograms, 200 pmol) have been purified to allow structural and functional analysis of the molecule and comparison with other know differentiation inducers...
  3. Apelgot S, Guille E. Treatment of mice bearing a Krebs ascitic tumor by means of a protocol based on radioactive copper (64Cu). III. Efficiency of the anticancer treatment according to the stage of tumor development. Anticancer Res. 1989;9:955-60 pubmed
  4. Guille E, Apelgot S. Treatment of mice bearing a Krebs ascitic tumor by means of a protocol based on radioactive copper (64Cu). II. Analysis of the treatment. Anticancer Res. 1989;9:947-53 pubmed
    ..Two different strains of mice were used (Swiss or CBA). For each of them, the same treatment was efficient on condition that thioproline or spermine was used for Swiss or CBA mice respectively...
  5. Yakubov L, Deeva E, Zarytova V, Ivanova E, Ryte A, Yurchenko L, et al. Mechanism of oligonucleotide uptake by cells: involvement of specific receptors?. Proc Natl Acad Sci U S A. 1989;86:6454-8 pubmed
    ..The polyanions heparin and chondroitin sulfates A and B do not inhibit binding. These observations suggest the involvement of specific receptor proteins in binding of oligomers to mammalian cells...
  6. Borovjagin A, Ezrokhi M, Rostapshov V, Ugarova TYu -, Bystrova T, Shatsky I. RNA--protein interactions within the internal translation initiation region of encephalomyocarditis virus RNA. Nucleic Acids Res. 1991;19:4999-5005 pubmed
    ..Implications of these findings for the mechanism of initiation resulting from internal entry of ribosomes are discussed...
  7. Hilton D, Nicola N, Waring P, Metcalf D. Clearance and fate of leukemia-inhibitory factor (LIF) after injection into mice. J Cell Physiol. 1991;148:430-9 pubmed
    ..The appearance of a large amount of nonprecipitable 125I in the urine suggested that the kidneys were the major route of LIF clearance from the body...
  8. Ji Y, Moog C, Schmitt G, Bischoff P, Luu B. Monophosphoric acid diesters of 7 beta-hydroxycholesterol and of pyrimidine nucleosides as potential antitumor agents: synthesis and preliminary evaluation of antitumor activity. J Med Chem. 1990;33:2264-70 pubmed
    ..These compounds were screened for their antiproliferative activity toward EL-4 murine leukemia cells in vitro and for their antitumor activity against the mice bearing Krebs II ascitic carcinoma in vivo...
  9. Grankowski N, Issinger O. Subcellular localization of casein kinase I. Biochem Biophys Res Commun. 1990;167:471-6 pubmed
    ..The mammalian CKI showed virtually the same molecular mass (app. 45 kDa) as the yeast enzyme. By immunofluorescence it could be shown that CKI is preferably located in the nucleolus...
  10. Pardoe I, Grewal K, Baldeh M, Hamid J, Burness A. Persistent infection of K562 cells by encephalomyocarditis virus. J Virol. 1990;64:6040-4 pubmed
    ..Resistance was due, at least in part, to reduced virus attachment in that binding of 3H-labeled virus to cloned resistant cells was about 2% of that to uninfected cells...
  11. Borovjagin A, Evstafieva A, Ugarova TYu -, Shatsky I. A factor that specifically binds to the 5'-untranslated region of encephalomyocarditis virus RNA. FEBS Lett. 1990;261:237-40 pubmed
    ..This set of transcripts has allowed us to find out that the factor (p58) binds to EMCV RNA within the sequence 315-485...
  12. Szyszka R, Grankowski N, Felczak K, Shugar D. Halogenated benzimidazoles and benzotriazoles as selective inhibitors of protein kinases CK I and CK II from Saccharomyces cerevisiae and other sources. Biochem Biophys Res Commun. 1995;208:418-24 pubmed
    ..All inhibitors are competitive with respect to ATP (and GTP with CK II), the two latter with Ki values in the range 0.2-0.6 microM for CK II from yeast and mammalian sources...
  13. Mathonnet G, Fabian M, Svitkin Y, Parsyan A, Huck L, Murata T, et al. MicroRNA inhibition of translation initiation in vitro by targeting the cap-binding complex eIF4F. Science. 2007;317:1764-7 pubmed
    ..Our results indicate that inhibition of translation initiation is the earliest molecular event effected by miRNAs. Other mechanisms, such as mRNA degradation, may subsequently consolidate mRNA silencing...
  14. Pryme I, Pusztai A, Bardocz S, Ewen S. The induction of gut hyperplasia by phytohaemagglutinin in the diet and limitation of tumour growth. Histol Histopathol. 1998;13:575-83 pubmed
    ..required for growth. Experiments with a second murine tumour cell line (a plasmacytoma) in Balb/c mice gave similar results indicating that the effect of PHA was not restricted to a single tumour system...
  15. Julia A, Canal P, Berg D, Bachaud J, Daly N, Bugat R. Concomitant evaluation of efficiency, acute and delayed toxicities of combined treatment of radiation and CDDP on an in vivo model. Int J Radiat Oncol Biol Phys. 1991;20:347-50 pubmed
    ..There was no delayed renal toxicity. The study on the Krebs II ascites model confirms the tumor cell therapeutic potentiation without exacerbation of normal tissue damage...
  16. Kleschyov A, Sedov K, Mordvintcev P, Vanin A. Biotransformation of sodium nitroprusside into dinitrosyl iron complexes in tissue of ascites tumors of mice. Biochem Biophys Res Commun. 1994;202:168-73 pubmed
    ..Thus, ascites tumor cells contribute to the transformation of SNP into DNIC and thiols are essential in this process...
  17. Apelgot S, Guille E. Treatment of mice bearing a Krebs ascitic tumor by means of a protocol based on radioactive copper (64Cu). I. The "cancer animal": a new concept leading to an effective antitumoral treatment. Anticancer Res. 1989;9:941-6 pubmed
    ..This treatment was efficient in curing mice injected 1 or 6 days previously with 5 x 10(5) Krebs ascitic cells...
  18. Vartapetian A, Makarova T, Koonin E, Agol V, Bogdanov A. Small cytoplasmic RNA from mouse cells covalently linked to a protein. FEBS Lett. 1988;232:35-8 pubmed
    ..The protein appears to be attached to the 5'-terminus of the RNA molecule, which is approx. 20 nucleotides long...
  19. Drygin YuF -, Shabanov A, Bogdanov A. An enzyme which specifically splits a covalent bond between picornaviral RNA and VPg. FEBS Lett. 1988;239:343-6 pubmed
    ..We found that the enzyme does not 'unlink' VPg from comoviral RNA. We suggest that Y-pUpN PDE represents a new class of enzymes...
  20. Quemener V, Quash G, Moulinoux J, Penlap V, Ripoll H, Havouis R, et al. In vivo antitumor activity of 4-amino 4-methyl 2-pentyne 1-al, an inhibitor of aldehyde dehydrogenase. In Vivo. 1989;3:325-30 pubmed
    ..A possible relationship between the ALDH isoenzyme activity of the tumor and its sensitivity to AMPAL is discussed in the light of previous reports concerning the role of aldehydes in cell growth control...
  21. Pilipenko E, Blinov V, Chernov B, Dmitrieva T, Agol V. Conservation of the secondary structure elements of the 5'-untranslated region of cardio- and aphthovirus RNAs. Nucleic Acids Res. 1989;17:5701-11 pubmed
    ..The possible involvement of the 5"-UTR secondary structure domains in the translational control is briefly discussed...
  22. Gingras A, Svitkin Y, Belsham G, Pause A, Sonenberg N. Activation of the translational suppressor 4E-BP1 following infection with encephalomyocarditis virus and poliovirus. Proc Natl Acad Sci U S A. 1996;93:5578-83 pubmed
    ..Dephosphorylation of 4E-BP1 by specifically inhibiting cap-dependent translation may be the major cause of the shutoff phenomenon in EMCV-infected cells...
  23. Bryans M, Kass S, Seivwright C, Adams R. Vector methylation inhibits transcription from the SV40 early promoter. FEBS Lett. 1992;309:97-102 pubmed
  24. Fabian M, Mathonnet G, Sundermeier T, Mathys H, Zipprich J, Svitkin Y, et al. Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation. Mol Cell. 2009;35:868-80 pubmed publisher
    ..Importantly, we present evidence that GW182, a core component of the miRISC, directly interacts with PABP via its C-terminal region and that this interaction is required for miRNA-mediated deadenylation...
  25. Terce F, Record M, Tronchere H, Ribbes G, Chap H. Cytidylyltransferase translocation onto endoplasmic reticulum and increased de novo synthesis without phosphatidylcholine accumulation in Krebs-II ascite cells. Biochim Biophys Acta. 1991;1084:69-77 pubmed
    ..Therefore, Krebs-II cells exhibited a high degree of regulation of their phosphatidylcholine content, suggesting a parallel stimulation of both synthesis and degradation...