myofibrils

Summary

Summary: The long cylindrical contractile organelles of STRIATED MUSCLE cells composed of ACTIN FILAMENTS; MYOSIN filaments; and other proteins organized in arrays of repeating units called SARCOMERES .

Top Publications

  1. Falcao Pires I, Palladini G, Gonçalves N, van der Velden J, Moreira Gonçalves D, Miranda Silva D, et al. Distinct mechanisms for diastolic dysfunction in diabetes mellitus and chronic pressure-overload. Basic Res Cardiol. 2011;106:801-14 pubmed publisher
    ..The association of these damages accelerates the progression of diastolic heart failure progression in diabetic-banded animals...
  2. Pontes Soares C, Midlej V, de Oliveira M, Benchimol M, Costa M, Mermelstein C. 2D and 3D-organized cardiac cells shows differences in cellular morphology, adhesion junctions, presence of myofibrils and protein expression. PLoS ONE. 2012;7:e38147 pubmed publisher
    ..cells, contraction ability, proliferation rate, presence of intercellular adhesion structures, organization of myofibrils, mitochondria morphology, endoplasmic reticulum contents, cytoskeletal filaments and extracellular matrix ..
  3. Kresh J, Chopra A. Intercellular and extracellular mechanotransduction in cardiac myocytes. Pflugers Arch. 2011;462:75-87 pubmed publisher
  4. Gokhin D, Fowler V. A two-segment model for thin filament architecture in skeletal muscle. Nat Rev Mol Cell Biol. 2013;14:113-9 pubmed publisher
    ..The two-segment model implicates position-specific microregulation of actin dynamics as a general principle underlying actin filament length and stability...
  5. Leung M, Hitchen P, Ward D, Messer A, Marston S. Z-band alternatively spliced PDZ motif protein (ZASP) is the major O-linked ?-N-acetylglucosamine-substituted protein in human heart myofibrils. J Biol Chem. 2013;288:4891-8 pubmed publisher
    ..05). ZASP is only 22% of all O-GlcNAcylated proteins in mouse heart myofibrils.
  6. Richard A, Demignon J, Sakakibara I, Pujol J, Favier M, Strochlic L, et al. Genesis of muscle fiber-type diversity during mouse embryogenesis relies on Six1 and Six4 gene expression. Dev Biol. 2011;359:303-20 pubmed publisher
    ..Six genes thus behave as essential global regulators of muscle gene expression, as well as a central switch to drive the skeletal muscle fast phenotype during fetal development...
  7. Grosberg A, Kuo P, Guo C, Geisse N, Bray M, Adams W, et al. Self-organization of muscle cell structure and function. PLoS Comput Biol. 2011;7:e1001088 pubmed publisher
    ..fibers, premyofibrils and focal adhesions, as well as cooperative alignment and parallel bundling of nascent myofibrils. Our results suggest that a hierarchy of mechanisms regulate the self-organization of the contractile ..
  8. Kurosaka S, Leu N, Pavlov I, Han X, Ribeiro P, Xu T, et al. Arginylation regulates myofibrils to maintain heart function and prevent dilated cardiomyopathy. J Mol Cell Cardiol. 2012;53:333-41 pubmed publisher
    ..These symptoms were accompanied by severe ultrastructural defects in cardiac myofibrils, seen in the newborns and far preceding the onset of cardiomyopathy, suggesting that these defects were primary ..
  9. Borejdo J, Szczesna Cordary D, Muthu P, Metticolla P, Luchowski R, Gryczynski Z, et al. Single molecule detection approach to muscle study: kinetics of a single cross-bridge during contraction of muscle. Methods Mol Biol. 2012;875:311-34 pubmed publisher
    ..of cross-bridge attachment and dissociation are significantly different in isometrically contracting cardiac myofibrils from right ventricle of WT and Tg-D166V mice...

More Information

Publications82

  1. Liu B, Lee R, Biesiadecki B, Tikunova S, Davis J. Engineered troponin C constructs correct disease-related cardiac myofilament calcium sensitivity. J Biol Chem. 2012;287:20027-36 pubmed publisher
    ..Thus, the present study provides a novel and versatile therapeutic strategy to restore diseased cardiac muscle Ca(2+) sensitivity...
  2. Lovelock J, Monasky M, Jeong E, Lardin H, Liu H, Patel B, et al. Ranolazine improves cardiac diastolic dysfunction through modulation of myofilament calcium sensitivity. Circ Res. 2012;110:841-50 pubmed publisher
    ..Oxidative stress has been shown to increase late inward sodium current (I(Na)), reducing the net cytosolic Ca(2+) efflux...
  3. Mellor K, Wendt I, Ritchie R, Delbridge L. Fructose diet treatment in mice induces fundamental disturbance of cardiomyocyte Ca2+ handling and myofilament responsiveness. Am J Physiol Heart Circ Physiol. 2012;302:H964-72 pubmed publisher
    ..These findings demonstrate that fructose diet-associated myocardial insulin resistance induces profound disturbance of cardiomyocyte Ca(2+) handling and responsiveness in the absence of altered systemic loading conditions...
  4. Li H, Xu J, Bian Y, Rotllant P, Shen T, Chu W, et al. Smyd1b_tv1, a key regulator of sarcomere assembly, is localized on the M-line of skeletal muscle fibers. PLoS ONE. 2011;6:e28524 pubmed publisher
    ..Smyd1b encodes two alternatively spliced isoforms, smyd1b_tv1 and smyd1b_tv2, that are expressed in skeletal and cardiac muscles and play a vital role in myofibrillogenesis in skeletal muscles of zebrafish embryos...
  5. Ermolova N, Kudryashova E, DiFranco M, VERGARA J, Kramerova I, Spencer M. Pathogenity of some limb girdle muscular dystrophy mutations can result from reduced anchorage to myofibrils and altered stability of calpain 3. Hum Mol Genet. 2011;20:3331-45 pubmed publisher
    ..Our data suggest that R448H and D705G mutations affect both CAPN3s anchorage to titin and its stability. These studies reveal a novel mechanism by which mutations that spare enzymatic activity can still lead to calpainopathy...
  6. Viswanathan M, Kaushik G, Engler A, Lehman W, Cammarato A. A Drosophila melanogaster model of diastolic dysfunction and cardiomyopathy based on impaired troponin-T function. Circ Res. 2014;114:e6-17 pubmed publisher
    ..The Drosophila upheld(101) Glu/Lys amino acid substitution lies C-terminally adjacent to this phylogenetically conserved sequence...
  7. Kaasik P, Riso E, Seene T. Extracellular matrix and myofibrils during unloading and reloading of skeletal muscle. Int J Sports Med. 2011;32:247-53 pubmed publisher
  8. Chopra A, Tabdanov E, Patel H, Janmey P, Kresh J. Cardiac myocyte remodeling mediated by N-cadherin-dependent mechanosensing. Am J Physiol Heart Circ Physiol. 2011;300:H1252-66 pubmed publisher
    ..These results indicate that cell-to-cell-mediated force perception and transmission are involved in the organization and development of cardiac structure and function...
  9. Selcen D. Myofibrillar myopathies. Neuromuscul Disord. 2011;21:161-71 pubmed publisher
    ..However, in the majority of the myofibrillar myopathy patients the disease gene awaits discovery...
  10. Mettikolla P, Calander N, Luchowski R, Gryczynski I, Gryczynski Z, Zhao J, et al. Cross-bridge kinetics in myofibrils containing familial hypertrophic cardiomyopathy R58Q mutation in the regulatory light chain of myosin. J Theor Biol. 2011;284:71-81 pubmed publisher
    ..We studied the effect of the FHC-linked R58Q-RLC mutation on the kinetics of transgenic (Tg)-R58Q cardiac myofibrils. Kinetics was determined from the rate of change of orientation of actin monomers during muscle contraction...
  11. Melkani G, Bodmer R, Ocorr K, Bernstein S. The UNC-45 chaperone is critical for establishing myosin-based myofibrillar organization and cardiac contractility in the Drosophila heart model. PLoS ONE. 2011;6:e22579 pubmed publisher
    ..Structural analysis showed reduced myofibrils, myofibrillar disarray, and greatly decreased cardiac myosin accumulation...
  12. Donlin L, Andresen C, Just S, Rudensky E, Pappas C, Kruger M, et al. Smyd2 controls cytoplasmic lysine methylation of Hsp90 and myofilament organization. Genes Dev. 2012;26:114-9 pubmed publisher
    ..Collectively, our data reveal a cytoplasmic protein network that employs lysine methylation for the maintenance and function of skeletal muscle...
  13. Little S, Biesiadecki B, Kilic A, Higgins R, Janssen P, Davis J. The rates of Ca2+ dissociation and cross-bridge detachment from ventricular myofibrils as reported by a fluorescent cardiac troponin C. J Biol Chem. 2012;287:27930-40 pubmed publisher
    ..dissociation from TnC and the rate of cross-bridge detachment from several different species of ventricular myofibrils. The fluorescently labeled TnC was sensitive to both Ca(2+) dissociation and cross-bridge detachment at low Ca(2+..
  14. Cohen S, Zhai B, Gygi S, Goldberg A. Ubiquitylation by Trim32 causes coupled loss of desmin, Z-bands, and thin filaments in muscle atrophy. J Cell Biol. 2012;198:575-89 pubmed publisher
    ..Thus, during fasting, desmin phosphorylation increases and enhances Trim32-mediated degradation of the desmin cytoskeleton, which appears to facilitate the breakdown of Z-bands and thin filaments...
  15. Hawkins T, Haramis A, Etard C, Prodromou C, Vaughan C, Ashworth R, et al. The ATPase-dependent chaperoning activity of Hsp90a regulates thick filament formation and integration during skeletal muscle myofibrillogenesis. Development. 2008;135:1147-56 pubmed publisher
    ..Our studies reveal a surprisingly specific developmental role for a single Hsp90 gene in a regulatory pathway controlling late steps in sarcomere assembly. ..
  16. Claeys K, van der Ven P, Behin A, Stojkovic T, Eymard B, Dubourg O, et al. Differential involvement of sarcomeric proteins in myofibrillar myopathies: a morphological and immunohistochemical study. Acta Neuropathol. 2009;117:293-307 pubmed publisher
  17. Ahuja P, Perriard E, Pedrazzini T, Satoh S, Perriard J, Ehler E. Re-expression of proteins involved in cytokinesis during cardiac hypertrophy. Exp Cell Res. 2007;313:1270-83 pubmed
    ..highly ordered and functional sarcomeres in the adult myocardium or could be because of the inefficiency of degradation pathways, which facilitate the division of differentiated embryonic cardiomyocytes by disintegrating myofibrils.
  18. Belus A, Piroddi N, Scellini B, Tesi C, D Amati G, Girolami F, et al. The familial hypertrophic cardiomyopathy-associated myosin mutation R403Q accelerates tension generation and relaxation of human cardiac myofibrils. J Physiol. 2008;586:3639-44 pubmed publisher
    ..Here we directly compare contraction and relaxation mechanics of single myofibrils from left ventricular samples of one patient carrying the R403Q mutation to those from a healthy control heart...
  19. Masiero E, Agatea L, Mammucari C, Blaauw B, Loro E, Komatsu M, et al. Autophagy is required to maintain muscle mass. Cell Metab. 2009;10:507-15 pubmed publisher
    ..Our results suggest that inhibition/alteration of autophagy can contribute to myofiber degeneration and weakness in muscle disorders characterized by accumulation of abnormal mitochondria and inclusions. ..
  20. Sosnovik D, Wang R, Dai G, Wang T, Aikawa E, Novikov M, et al. Diffusion spectrum MRI tractography reveals the presence of a complex network of residual myofibers in infarcted myocardium. Circ Cardiovasc Imaging. 2009;2:206-12 pubmed publisher
    ..DSI tractography thus provides a new and important readout of tissue injury after myocardial infarction. ..
  21. Bang M, Li X, Littlefield R, Bremner S, Thor A, Knowlton K, et al. Nebulin-deficient mice exhibit shorter thin filament lengths and reduced contractile function in skeletal muscle. J Cell Biol. 2006;173:905-16 pubmed
    ..The functional importance of nebulin in skeletal muscle function was revealed by isometric contractility assays, which demonstrated a dramatic reduction in force production in nebulin-deficient skeletal muscle. ..
  22. Olive M. Extralysosomal protein degradation in myofibrillar myopathies. Brain Pathol. 2009;19:507-15 pubmed publisher
    ..a group of heterogeneous muscle disorders morphologically defined by the presence of foci of dissolution of the myofibrils, accumulation of the products of myofibrillar degradation and ectopic expression of multiple proteins...
  23. Kavazis A, McClung J, Hood D, Powers S. Exercise induces a cardiac mitochondrial phenotype that resists apoptotic stimuli. Am J Physiol Heart Circ Physiol. 2008;294:H928-35 pubmed
    ..Furthermore, these results are consistent with the concept that exercise-induced mitochondrial adaptations contribute to exercise-induced cardioprotection. ..
  24. Bernick E, Zhang P, Du S. Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos. BMC Cell Biol. 2010;11:70 pubmed publisher
    ..Collectively, these studies indicate that the expression levels of Unc-45b must be precisely regulated to ensure normal myofibril organization. Loss or overexpression of Unc-45b leads to defective myofibril organization. ..
  25. Gilbert S, Benson A, Li P, Holden A. Regional localisation of left ventricular sheet structure: integration with current models of cardiac fibre, sheet and band structure. Eur J Cardiothorac Surg. 2007;32:231-49 pubmed
  26. Baltgalvis K, Berger F, Pena M, Davis J, White J, Carson J. Muscle wasting and interleukin-6-induced atrogin-I expression in the cachectic Apc ( Min/+ ) mouse. Pflugers Arch. 2009;457:989-1001 pubmed publisher
    ..These data suggest that high circulating IL-6 levels induce type IIB fiber CSA loss in Apc ( Min/+ ) mice, and circulating IL-6 is sufficient to regulate Atrogin-I gene expression in cachectic mice. ..
  27. Paulsen G, Vissing K, Kalhovde J, Ugelstad I, Bayer M, Kadi F, et al. Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans. Am J Physiol Regul Integr Comp Physiol. 2007;293:R844-53 pubmed
    ..Later, mRNA and total HSP protein levels, especially HSP70, increased, indicating that HSPs play a role in skeletal muscle recovery and remodeling/adaptation processes to high-force exercise. ..
  28. Stoecker U, Telley I, Stussi E, Denoth J. A multisegmental cross-bridge kinetics model of the myofibril. J Theor Biol. 2009;259:714-26 pubmed publisher
    ..muscle is a complex biological system; a single mammalian muscle fibre contains up to hundred or even more myofibrils in parallel connected via an inter-myofibril filament network...
  29. Stehle R, Solzin J, Iorga B, Poggesi C. Insights into the kinetics of Ca2+-regulated contraction and relaxation from myofibril studies. Pflugers Arch. 2009;458:337-57 pubmed publisher
    ..techniques that analyse the chemical and mechanical kinetics of small components of a muscle fibre, subcellular myofibrils isolated from skeletal and cardiac muscle...
  30. Kronert W, Melkani G, Melkani A, Bernstein S. Mutating the converter-relay interface of Drosophila myosin perturbs ATPase activity, actin motility, myofibril stability and flight ability. J Mol Biol. 2010;398:625-32 pubmed publisher
    ..Fibers from 1-week-old adults show more severe cracking and frayed myofibrils with some disruption of the myofilament lattice...
  31. Nakashima K, Yakabe Y. AMPK activation stimulates myofibrillar protein degradation and expression of atrophy-related ubiquitin ligases by increasing FOXO transcription factors in C2C12 myotubes. Biosci Biotechnol Biochem. 2007;71:1650-6 pubmed
    ..These results indicate that activation of AMPK stimulates myofibrillar protein degradation through the expression of atrogin-1/MAFbx and MuRF1 by increasing FOXO transcription factors in skeletal muscles. ..
  32. Schroder R, Schoser B. Myofibrillar myopathies: a clinical and myopathological guide. Brain Pathol. 2009;19:483-92 pubmed publisher
    ..This review focuses on the clinical and myopathological aspects of genetically defined MFMs, and shall provide a diagnostic guide for this numerically significant group of protein aggregate myopathies. ..
  33. Miller R, Qadota H, Landsverk M, Mercer K, Epstein H, Benian G. UNC-98 links an integrin-associated complex to thick filaments in Caenorhabditis elegans muscle. J Cell Biol. 2006;175:853-9 pubmed
    ..In vertebrate muscle, focal adhesion-like structures called costameres attach myofibrils at the periphery of muscle cells to the cell membrane...
  34. Zieseniss A, Terasaki A, Gregorio C. Lasp-2 expression, localization, and ligand interactions: a new Z-disc scaffolding protein. Cell Motil Cytoskeleton. 2008;65:59-72 pubmed
    ..embryonic stage 25 and lasp-2 protein was associated with developing premyofibril structures, Z-discs of mature myofibrils, focal adhesions, and intercalated discs of cultured cardiomyocytes...
  35. Sanger J, Wang J, Fan Y, White J, Sanger J. Assembly and dynamics of myofibrils. J Biomed Biotechnol. 2010;2010:858606 pubmed publisher
    We review some of the problems in determining how myofibrils may be assembled and just as importantly how this contractile structure may be renewed by sarcomeric proteins moving between the sarcomere and the cytoplasm...
  36. Sanger J, Wang J, Holloway B, Du A, Sanger J. Myofibrillogenesis in skeletal muscle cells in zebrafish. Cell Motil Cytoskeleton. 2009;66:556-66 pubmed publisher
    ..model of myofibrillogenesis, based on observations in cultured avian muscle cells, proposes that mature myofibrils are preceded by two intermediary structures: premyofibrils and nascent myofibrils...
  37. Shinde A, Nakano S, Sugawara M, Toyoshima I, Ito H, Tanaka K, et al. Expression of caveolar components in primary desminopathy. Neuromuscul Disord. 2008;18:215-9 pubmed publisher
    ..Alternatively, they could be trapped during internalization. We hypothesize that the accumulation of multiple proteins in MFM could be partially due to inhibited intracellular trafficking...
  38. Shimamoto Y, Kono F, Suzuki M, Ishiwata S. Nonlinear force-length relationship in the ADP-induced contraction of skeletal myofibrils. Biophys J. 2007;93:4330-41 pubmed
    ..from the effect of Ca(2+), we investigated the force-sarcomere length (SL) relationship in rabbit skeletal myofibrils (a single myofibril or a thin bundle) at SL > 2...
  39. Bär H, Mücke N, Ringler P, Müller S, Kreplak L, Katus H, et al. Impact of disease mutations on the desmin filament assembly process. J Mol Biol. 2006;360:1031-42 pubmed
  40. Hosoda A, Sato N, Nagaoka R, Abe H, Obinata T. Activity of cofilin can be regulated by a mechanism other than phosphorylation/dephosphorylation in muscle cells in culture. J Muscle Res Cell Motil. 2007;28:183-94 pubmed
    ..Our results suggest that the activity of A3-cofilin and also S3-cofilin can be regulated by PIP(2) in the cytoplasm of muscle cells...
  41. Vendelin M, Hoerter J, Mateo P, Soboll S, Gillet B, Mazet J. Modulation of energy transfer pathways between mitochondria and myofibrils by changes in performance of perfused heart. J Biol Chem. 2010;285:37240-50 pubmed publisher
    In the heart, the energy supplied by mitochondria to myofibrils is continuously and finely tuned to the contraction requirement over a wide range of cardiac loads...
  42. Zhang C, Jia P, Huang X, Sferrazza G, Athauda G, Achary M, et al. Myofibril-inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts. J Biomed Sci. 2009;16:81 pubmed publisher
    ..results in a failure of homozygous (c/c) embryos to form hearts that beat because of an absence of organized myofibrils. Our previous studies have shown that a noncoding RNA, Myofibril-Inducing RNA (MIR), is capable of promoting ..
  43. Paulsen G, Lauritzen F, Bayer M, Kalhovde J, Ugelstad I, Owe S, et al. Subcellular movement and expression of HSP27, alphaB-crystallin, and HSP70 after two bouts of eccentric exercise in humans. J Appl Physiol (1985). 2009;107:570-82 pubmed publisher
    ..The large amount of HSP27, alphaB-crystallin, and HSP70 in the cytoskeletal/myofibrillar fraction after a repeated bout of exercise suggests a protective role as part of the repeated-bout effect...
  44. Joumaa V, Rassier D, Leonard T, Herzog W. Passive force enhancement in single myofibrils. Pflugers Arch. 2007;455:367-71 pubmed publisher
    ..further insight into passive force enhancement by testing whether passive force enhancement occurs in single myofibrils. Myofibrils (n = 6) isolated from rabbit psoas muscle were fixed at a sarcomere length of 2...
  45. McKeown C, Nowak R, Moyer J, Sussman M, Fowler V. Tropomodulin1 is required in the heart but not the yolk sac for mouse embryonic development. Circ Res. 2008;103:1241-8 pubmed publisher
    Tropomodulin (Tmod)1 caps the pointed ends of actin filaments in sarcomeres of striated muscle myofibrils and in the erythrocyte membrane skeleton...
  46. Scellini B, Piroddi N, Poggesi C, Tesi C. Extraction and replacement of the tropomyosin-troponin complex in isolated myofibrils. Adv Exp Med Biol. 2010;682:163-74 pubmed publisher
    ..Here we describe the method we recently developed to replace Tm-Tn of skeletal and cardiac myofibrils from animals and humans to generate an experimental model of homogeneous Tm composition and giving the ..
  47. Bray M, Sheehy S, Parker K. Sarcomere alignment is regulated by myocyte shape. Cell Motil Cytoskeleton. 2008;65:641-51 pubmed publisher
    ..Furthermore, geometric boundaries such as corners induce localized myofibrillar anisotropy that becomes global as the myocyte aspect ratio increases...
  48. Kagawa M, Sato N, Obinata T. Effects of BTS (N-benzyl-p-toluene sulphonamide), an inhibitor for myosin-actin interaction, on myofibrillogenesis in skeletal muscle cells in culture. Zoolog Sci. 2006;23:969-75 pubmed
    ..These results show that actin-myosin interaction plays a critical role in the process of myofibrillogenesis...
  49. Ottenheijm C, Witt C, Stienen G, Labeit S, Beggs A, Granzier H. Thin filament length dysregulation contributes to muscle weakness in nemaline myopathy patients with nebulin deficiency. Hum Mol Genet. 2009;18:2359-69 pubmed publisher
  50. Huang W, Zhang R, Xu X. Myofibrillogenesis in the developing zebrafish heart: A functional study of tnnt2. Dev Biol. 2009;331:237-49 pubmed publisher
    ..In summary, our data indicates that zebrafish are a valuable in vivo model for studying both myofibrillogenesis and sarcomere-based cardiac diseases...
  51. Mercer K, Miller R, Tinley T, Sheth S, Qadota H, Benian G. Caenorhabditis elegans UNC-96 is a new component of M-lines that interacts with UNC-98 and paramyosin and is required in adult muscle for assembly and/or maintenance of thick filaments. Mol Biol Cell. 2006;17:3832-47 pubmed
    ..Additionally, UNC-96 copurifies with native thick filaments. A model is presented in which UNC-96 is required in adult muscle to promote thick filament assembly and/or maintenance...
  52. Du A, Sanger J, Sanger J. Cardiac myofibrillogenesis inside intact embryonic hearts. Dev Biol. 2008;318:236-46 pubmed publisher
    How proteins assemble into sarcomeric arrays to form myofibrils is controversial...
  53. Siththanandan V, Tobacman L, Van Gorder N, Homsher E. Mechanical and kinetic effects of shortened tropomyosin reconstituted into myofibrils. Pflugers Arch. 2009;458:761-76 pubmed publisher
    The effects of tropomyosin on muscle mechanics and kinetics were examined in skeletal myofibrils using a novel method to remove tropomyosin (Tm) and troponin (Tn) and then replace these proteins with altered versions...
  54. Shimamoto Y, Suzuki M, Ishiwata S. Length-dependent activation and auto-oscillation in skeletal myofibrils at partial activation by Ca2+. Biochem Biophys Res Commun. 2008;366:233-8 pubmed
    The length-dependent activation of skeletal myofibrils was examined at the single-sarcomere level with phase-contrast microscopy at sarcomere length (SL) >2.2 microm. At the maximal activation by Ca(2+) (pCa 4...
  55. Solzin J, Iorga B, Sierakowski E, Gomez Alcazar D, Ruess D, Kubacki T, et al. Kinetic mechanism of the Ca2+-dependent switch-on and switch-off of cardiac troponin in myofibrils. Biophys J. 2007;93:3917-31 pubmed
    ..changes of human cardiac troponin (cTn) were studied on isolated cTn and within the sarcomeric environment of myofibrils. Human cTnC was selectively labeled on cysteine 84 with N-((2-(iodoacetoxy)ethyl)-N-methyl)amino-7-nitrobenz-2-..
  56. Cohen S, Brault J, Gygi S, Glass D, Valenzuela D, Gartner C, et al. During muscle atrophy, thick, but not thin, filament components are degraded by MuRF1-dependent ubiquitylation. J Cell Biol. 2009;185:1083-95 pubmed publisher
    ..Their selective loss requires MuRF1. MyHC is protected from ubiquitylation in myofibrils by associated proteins, but eventually undergoes MuRF1-dependent degradation...
  57. Lecker S, Goldberg A, Mitch W. Protein degradation by the ubiquitin-proteasome pathway in normal and disease states. J Am Soc Nephrol. 2006;17:1807-19 pubmed
  58. Kruger M, Kötter S, Grützner A, Lang P, Andresen C, Redfield M, et al. Protein kinase G modulates human myocardial passive stiffness by phosphorylation of the titin springs. Circ Res. 2009;104:87-94 pubmed publisher
    ..determine whether PKG can phosphorylate titin and affect titin-based stiffness in skinned myofibers and isolated myofibrils. PKG in the presence of 8-pCPT-cGMP (cGMP) phosphorylated the 2 main cardiac titin isoforms, N2BA and N2B, in ..
  59. Joubert F, Wilding J, Fortin D, Domergue Dupont V, Novotova M, Ventura Clapier R, et al. Local energetic regulation of sarcoplasmic and myosin ATPase is differently impaired in rats with heart failure. J Physiol. 2008;586:5181-92 pubmed publisher
    ..that failing cardiomyocytes had intracellular disorganization, with fewer contacts between mitochondria and myofibrils. Despite normal SERCA protein content, spontaneous Ca2+ release measurements using Fluo-4 on saponin-..
  60. Wang J, Sanger J, Kang S, Thurston H, Abbott L, Dube D, et al. Ectopic expression and dynamics of TPM1alpha and TPM1kappa in myofibrils of avian myotubes. Cell Motil Cytoskeleton. 2007;64:767-76 pubmed
    ..coupled to chicken TPM1alpha and chicken TPM1kappa and compared their localizations in premyofibrils and mature myofibrils. We used the technique of fluorescence recovery after photobleaching (FRAP) to compare the dynamics of TPM1alpha ..
  61. Pavlov I, Novinger R, Rassier D. The mechanical behavior of individual sarcomeres of myofibrils isolated from rabbit psoas muscle. Am J Physiol Cell Physiol. 2009;297:C1211-9 pubmed publisher
    ..Single myofibrils from rabbit psoas were transferred into a temperature-controlled (22 degrees C or 15 degrees C) experimental ..
  62. Kontrogianni Konstantopoulos A, Ackermann M, Bowman A, Yap S, Bloch R. Muscle giants: molecular scaffolds in sarcomerogenesis. Physiol Rev. 2009;89:1217-67 pubmed publisher
  63. Iorga B, Blaudeck N, Solzin J, Neulen A, Stehle I, Lopez Davila A, et al. Lys184 deletion in troponin I impairs relaxation kinetics and induces hypercontractility in murine cardiac myofibrils. Cardiovasc Res. 2008;77:676-86 pubmed
  64. Edes I, Toth A, Csanyi G, Lomnicka M, Chłopicki S, Edes I, et al. Late-stage alterations in myofibrillar contractile function in a transgenic mouse model of dilated cardiomyopathy (Tgalphaq*44). J Mol Cell Cardiol. 2008;45:363-72 pubmed publisher
    ..In conclusion, our data suggested that an initial decrease of PKA signaling and subsequent changes in myofilament protein expression may contribute to the development of dilated cardiomyopathy in Tgalphaq*44 hearts...
  65. Tonino P, Pappas C, Hudson B, Labeit S, Gregorio C, Granzier H. Reduced myofibrillar connectivity and increased Z-disk width in nebulin-deficient skeletal muscle. J Cell Sci. 2010;123:384-91 pubmed publisher
    ..by using a recently developed nebulin knockout (KO) mouse model and measuring Z-disk displacement in adjacent myofibrils of both extensor digitorum longus (EDL) and soleus muscle...
  66. Parker K, Tan J, Chen C, Tung L. Myofibrillar architecture in engineered cardiac myocytes. Circ Res. 2008;103:340-2 pubmed publisher
    ..These data suggest that physical constrainment of muscle cells by extracellular matrix may be an important regulator of myofibrillar organization...
  67. Littlefield R, Fowler V. Thin filament length regulation in striated muscle sarcomeres: pointed-end dynamics go beyond a nebulin ruler. Semin Cell Dev Biol. 2008;19:511-9 pubmed publisher
    ..We present a unified model for thin filament length regulation where these two mechanisms cooperate to tailor thin filament lengths for specific contractile environments in diverse muscles...
  68. Selcen D. Myofibrillar myopathies. Curr Opin Neurol. 2008;21:585-9 pubmed publisher
    ..The aim of this communication is to provide an up-to-date overview of myofibrillar myopathies...
  69. Du S, Li H, Bian Y, Zhong Y. Heat-shock protein 90alpha1 is required for organized myofibril assembly in skeletal muscles of zebrafish embryos. Proc Natl Acad Sci U S A. 2008;105:554-9 pubmed publisher
    ..Knockdown of Hsp90alpha1 resulted in paralyzed zebrafish embryos with poorly organized myofibrils in skeletal muscles...
  70. Kulikovskaya I, McClellan G, Levine R, Winegrad S. Multiple forms of cardiac myosin-binding protein C exist and can regulate thick filament stability. J Gen Physiol. 2007;129:419-28 pubmed
    ..We hypothesize that modulation of filament stability can be coupled at the molecular level with the strength of contraction by the sensitivity of each to the concentration of calcium ions...
  71. Ehler E, Gautel M. The sarcomere and sarcomerogenesis. Adv Exp Med Biol. 2008;642:1-14 pubmed
    ..Studies in cell culture and developing embryos have revealed common pathways of sarcomere assembly in heart and skeletal muscle. Disruptions in these pathways are implicated in muscle diseases...
  72. Abraham T, Jones M, Kazmierczak K, Liang H, Pinheiro A, Wagg C, et al. Diastolic dysfunction in familial hypertrophic cardiomyopathy transgenic model mice. Cardiovasc Res. 2009;82:84-92 pubmed publisher
  73. Passarelli C, Di Venere A, Piroddi N, Pastore A, Scellini B, Tesi C, et al. Susceptibility of isolated myofibrils to in vitro glutathionylation: Potential relevance to muscle functions. Cytoskeleton (Hoboken). 2010;67:81-9 pubmed publisher
    In this study we investigated the molecular mechanism of glutathionylation on isolated human cardiac myofibrils using several pro-glutathionylating agents...