limit of detection


Summary: Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.

Top Publications

  1. Bjerke M, Andreasson U, Kuhlmann J, Portelius E, Pannee J, Lewczuk P, et al. Assessing the commutability of reference material formats for the harmonization of amyloid-? measurements. Clin Chem Lab Med. 2016;54:1177-91 pubmed publisher
    ..We conclude that the neat CSF candidate CRM can be used for value assignment of the kit calibrators for the different A?42 methods. ..
  2. Bolijn R, Op de Coul E, van Sighem A, Blok W, Kretzschmar M, Heijne J. Comparing viral load metrics and evaluating their use for HIV surveillance. J Infect. 2017;75:169-178 pubmed publisher
    ..VL metrics may be of value for enhancing HIV surveillance by identifying subgroup differences in impact of treatment on viral suppression, and by predicting numbers of new diagnoses in subsequent years. ..
  3. Mercier Delarue S, Durier C, Colin de Verdiere N, Poveda J, Meiffredy V, Fernandez Garcia M, et al. Screening test for neutralizing antibodies against yellow fever virus, based on a flavivirus pseudotype. PLoS ONE. 2017;12:e0177882 pubmed publisher
    ..This WN/YF17D test will be particularly useful for large epidemiological studies and for screening for neutralizing antibodies against yellow fever virus. ..
  4. Globisch D, Eubanks L, Shirey R, Pfarr K, Wanji S, Debrah A, et al. Validation of onchocerciasis biomarker N-acetyltyramine-O-glucuronide (NATOG). Bioorg Med Chem Lett. 2017;27:3436-3440 pubmed publisher
    ..Based on these extended studies, we suggest NATOG as a biomarker for tracking active onchocerciasis infections and provide a threshold concentration value of NATOG for future diagnostic tool development. ..
  5. Fontanarosa C, Pane F, Sepe N, Pinto G, Trifuoggi M, Squillace M, et al. Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry. PLoS ONE. 2017;12:e0179748 pubmed publisher
  6. Kim C, Searson P. Detection of Plasmodium Lactate Dehydrogenase Antigen in Buffer Using Aptamer-Modified Magnetic Microparticles for Capture, Oligonucleotide-Modified Quantum Dots for Detection, and Oligonucleotide-Modified Gold Nanoparticles for Signal Amplification. Bioconjug Chem. 2017;28:2230-2234 pubmed publisher
    ..We then demonstrate a detection limit of 10 amole (50-fold amplification) using oligonucleotide-functionalized gold nanoparticles to allow the conjugation of multiple quantum dots for each target antigen. ..
  7. Verma S, Singh R, Sharma V, Bumb R, Negi N, Ramesh V, et al. Development of a rapid loop-mediated isothermal amplification assay for diagnosis and assessment of cure of Leishmania infection. BMC Infect Dis. 2017;17:223 pubmed publisher
    ..The study demonstrated a Leishmania genus specific closed tube LAMP assay for reliable and rapid molecular diagnosis of VL and PKDL with potential for application in assessment of cure. ..
  8. Qiang Y, Wang W, Dhodary B, Yang J. Zeolitic imidazolate framework 8 (ZIF-8) reinforced macroporous resin D101 for selective solid-phase extraction of 1-naphthol and 2-naphthol from phenol compounds. Electrophoresis. 2017;38:1685-1692 pubmed publisher
    ..2-106.9%. This study indicated that D101@ZIF-8 (where ZIF is zeolitic imidazolate framework) is a promising selective adsorbent for the analysis of 1-naphthol and 2-naphthol in urine samples. ..
  9. Li S, Shi M, Zhao J, Zhang L, Huang Y, Zhao S. A highly sensitive capillary electrophoresis immunoassay strategy based on dual-labeled gold nanoparticles enhancing chemiluminescence for the detection of prostate-specific antigen. Electrophoresis. 2017;38:1780-1787 pubmed publisher
    ..The obtained results demonstrated that the proposed CE method may serve as an alternative tool for clinical analysis of PSA. ..

More Information

Publications106 found, 100 shown here

  1. Vikingsson S, Dahlberg J, Hansson J, Höiom V, Gréen H. Simple and cost-effective liquid chromatography-mass spectrometry method to measure dabrafenib quantitatively and six metabolites semi-quantitatively in human plasma. Anal Bioanal Chem. 2017;409:3749-3756 pubmed publisher
    ..Graphical abstract Combined multi reaction monitoring transitions of dabrafenib and metabolites in a typical case sample. ..
  2. Li X, Wen J, Jiang J, Zhao X, Zhou T, Fan G. Quantification of a Novel Photosensitizer of Chlorin e6-C15-Monomethyl Ester in Beagle Dog Plasma Using HPLC: Application to Pharmacokinetic Studies. Molecules. 2017;22: pubmed publisher
    ..5% to 102.8% and precisions (RSD) were within 6.8%. The validated method was successfully applied to the pharmacokinetic study of CMME after intravenous administration of single and multiple doses in beagle dogs. ..
  3. Mac Lochlainn D, Hira Kazal R, Varney H, Maher J. Implementation of National Institute for Health and Care Excellence (NICE) guidance to measure immunoglobulin A with all coeliac screens: can an affordable solution be devised?. Clin Exp Immunol. 2017;189:352-358 pubmed publisher
    ..However, this approach requires careful individualized validation, given the divergent efficiency with which assays identify samples containing low IgA. ..
  4. Pang Y, Tan Y, Ho H. Investigation of the effect of plasma albumin levels on regorafenib-induced hepatotoxicity using a validated liquid chromatography-tandem mass spectrometry method. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1061-1062:220-224 pubmed publisher
    ..The results indicated that physiologically-relevant levels of albumin were found to have no significant effect on the extent of protein binding of regorafenib, hence imposing minimal effect on drug disposition. ..
  5. Pei M, Huang X. Preparation and evaluation of an adsorbent based on poly (muconic acid-co-divinylbenzene/ethylenedimethacrylate) for multiple monolithic fiber solid-phase microextraction of tetracycline antibiotics. J Chromatogr A. 2017;1517:1-8 pubmed publisher
    ..5?g/kg and 20.0?g/kg spiking concentrations were 5.0-9.5% and 3.6-10.0%, respectively. The mean recoveries of the target TAs in the real honey samples were between 70.5-111.0%. ..
  6. Huo S, An H, Yu J, Mao X, Zhang Z, Bai L, et al. Pyrolytic in situ magnetization of metal-organic framework MIL-100 for magnetic solid-phase extraction. J Chromatogr A. 2017;1517:18-25 pubmed publisher
    ..5% to 106.6%. The results showed that the special anion-? orbital (electron donor-acceptor) interaction and ?-? stacking between magnetic MIL-100 and PAHs play an important role in the adsorption of PAHs. ..
  7. Zhao F, She Y, Zhang C, Cao X, Wang S, Zheng L, et al. Selective solid-phase extraction based on molecularly imprinted technology for the simultaneous determination of 20 triazole pesticides in cucumber samples using high-performance liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1064:143-150 pubmed publisher
    ..3% to 117.6% with relative standard deviations of less than 11.8% (n=5) for all analytes. The limits of detection for the 20 triazole pesticides were all less than 0.4?gL-1, and were sufficient to meet international standards. ..
  8. Wang S, Li W, Chang K, Liu J, Guo Q, Sun H, et al. Localized surface plasmon resonance-based abscisic acid biosensor using aptamer-functionalized gold nanoparticles. PLoS ONE. 2017;12:e0185530 pubmed publisher
    ..The proposed LSPR method could offer a new analytical platform for the detection of other plant hormones by changing the corresponding aptamer. ..
  9. Yang C, Yin X, Dong X, Zhang X, You L, Wang W, et al. Determination of the phytochemical composition of Jingning fang and the in vivo pharmacokinetics of its metabolites in rat plasma by UPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1067:71-88 pubmed publisher
    ..These findings are useful for predicting the bioactive components of JNF, and will aid in optimizing dose regimens of the drug...
  10. Oguma T, Nagaoka T, Kurahashi M, Kobayashi N, Yamamori S, Tsuji C, et al. Clinical contributions of exhaled volatile organic compounds in the diagnosis of lung cancer. PLoS ONE. 2017;12:e0174802 pubmed publisher
    ..06 and 0.03, respectively). Measurements of exhaled VOCs by a double cold-trap method may help diagnose lung cancer and monitor its progression and regression. ..
  11. Kadi A, Alrabiah H, Attwa M, Attia S, Mostafa G. Development and validation of HPLC-MS/MS method for the determination of lixivaptan in mouse plasma and its application in a pharmacokinetic study. Biomed Chromatogr. 2017;31: pubmed publisher
    ..The mean recovery of LIX from mouse plasma was 99.2?±?0.68%. All validation parameters for LIX were within the levels required for acceptance. The proposed method was effectively used for a pharmacokinetic study of LIX in mouse plasma. ..
  12. Cervantes Torres J, Segura Velázquez R, Padilla P, Sciutto E, Fragoso G. High stability of the immunomodulatory GK-1 synthetic peptide measured by a reversed phase high-performance liquid chromatography method. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1060:97-102 pubmed publisher
    ..Results shown in this study sustain the high stability of the GK-1 peptide using a reliable new selective and precise method suitable for its analysis. ..
  13. Rashidi Nodeh H, Sereshti H, Gaikani H, Kamboh M, Afsharsaveh Z. Magnetic graphene coated inorganic-organic hybrid nanocomposite for enhanced preconcentration of selected pesticides in tomato and grape. J Chromatogr A. 2017;1509:26-34 pubmed publisher
    ..83%, n=3) and inter-day (6.68-8.37%, n=12) precisions. Finally, the adsorbent was applied to determination of pesticides in tomato and grape samples and good recoveries were obtained in the range from 82 to 113% (RSDs 5.1-8.1%, n=3). ..
  14. Abdolhosseini S, Ghiasvand A, Heidari N. A high area, porous and resistant platinized stainless steel fiber coated by nanostructured polypyrrole for direct HS-SPME of nicotine in biological samples prior to GC-FID quantification. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1061-1062:5-10 pubmed publisher
    ..6%. The results demonstrated the superiority of the proposed fiber compared with the most used commercial types. The proposed HS-SPME-GC-FID method was successfully used for the analysis of nicotine in urine and human plasma samples. ..
  15. Santivañez Veliz M, Moreno Viguri E, Perez Silanes S, Varela J, Cerecetto H, Gonzalez M, et al. Development, validation and application of a GC-MS method for the simultaneous detection and quantification of neutral lipid species in Trypanosoma cruzi. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1061-1062:225-232 pubmed publisher
    ..Therefore, it is an interesting and useful contribution to medicinal chemistry research. ..
  16. Mane S, Bringans S, Johnson S, Pareek V, Utikar R. Reverse phase HPLC method for detection and quantification of lupin seed ?-conglutin. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1063:123-129 pubmed publisher
    ..The method validation findings were reproducible and can be successfully applied for routine analysis of ?-conglutin from lupin seed extract. ..
  17. Jasiecka Mikołajczyk A, Jaroszewski J. Determination of tigecycline in turkey plasma by LC-MS/MS: validation and application in a pharmacokinetic study. Pol J Vet Sci. 2017;20:241-249 pubmed publisher
    ..method was validated in terms of specificity, selectivity, linearity, lowest limit of quantification, limit of detection, precision, accuracy, matrix effect, carry-over effect, extraction recovery and stability...
  18. Melucci D, Locatelli M, Locatelli C. Trace level voltammetric determination of heavy metals and total mercury in tea matrices (Camellia sinensis). Food Chem Toxicol. 2013;62:901-7 pubmed
    ..Once set up on the standard reference materials, the analytical procedure was applied to commercial tea leaves samples. A critical comparison with spectroscopic measurements is also discussed. ..
  19. Kim J, Tran T, Hong S, Jeong J. A reference measurement procedure for amino acids in blood using isotope dilution ultra-performance liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1055-1056:72-80 pubmed publisher
    ..1% v/v trifluoroacetic acid. The limit of detection range of this method was 2-52fmol, and the RSDs of accuracy and precision from intra- and inter-day assays ..
  20. Moroncini G, Cuccioloni M, Mozzicafreddo M, Pozniak K, Grieco A, Paolini C, et al. Characterization of binding and quantification of human autoantibodies to PDGFR? using a biosensor-based approach. Anal Biochem. 2017;528:26-33 pubmed publisher
  21. Deng Q, Qiu M, Wang Y, Lv P, Wu C, Sun L, et al. A sensitive and validated immunomagnetic-bead based enzyme-linked immunosorbent assay for analyzing total T-2 (free and modified) toxins in shrimp tissues. Ecotoxicol Environ Saf. 2017;142:441-447 pubmed publisher
    ..2mg/kg. Our research showed a combination of ELISA and immunomagnetic bead technology provide a new, convenient approach to significantly improve the accuracy and sensitivity of total T-2 measurement in shrimp tissues. ..
  22. Ashraf M, Ghalloo B, Hayat M, Rahman J, Ejaz S, Iqbal M, et al. Determination of nitrate in biological fluids by HPLC. Pak J Pharm Sci. 2017;30:135-142 pubmed
    ..The method was used successfully for the estimation of nitrate in biological fluids like serum, CSF and ASF of 20 patients each. This is an alternate and reproducible method for the detection of nitrates in biological fluids...
  23. Smoluch M, Reszke E, Ramsza A, Labuz K, Silberring J. Direct analysis of methcathinone from crude reaction mixture by flowing atmospheric-pressure afterglow mass spectrometry. Rapid Commun Mass Spectrom. 2012;26:1577-80 pubmed publisher
  24. Baj Rossi C, De Micheli G, Carrara S. Electrochemical biochip for applications to wireless and batteryless monitoring of free-moving mice. Conf Proc IEEE Eng Med Biol Soc. 2014;2014:2020-3 pubmed publisher be capable of measuring several parameters: lactate with a sensitivity of 77±26 ?A/mM· cm(2) and a limit of detection (LOD) of 4±1 ?M; glucose with a sensitivity of 63±15 ?A/mM· cm(2) and a LOD of 8±2 ?M; Etoposide (a well ..
  25. Almqvist H, Sandahl M, Liden G. A rapid method for analysis of fermentatively produced D-xylonate using ultra-high performance liquid chromatography and evaporative light scattering detection. Biosci Biotechnol Biochem. 2017;81:1078-1080 pubmed publisher
    ..Separation of d-xylonate from other components of the fermentation medium was achieved. The dynamic range of the method was 0.2-7.0 g/L. ..
  26. Xiu Ping Z, Lin M, Lan Qi H, Jian Bo C, Li Z. The optimization and establishment of QuEChERS-UPLC-MS/MS method for simultaneously detecting various kinds of pesticides residues in fruits and vegetables. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1060:281-290 pubmed publisher
    ..2%-134.3% except pymetrozine and cyromazine with the relative standard deviations (RSD) of 1.0%-13.8%; the limit of detection (LOD) under three times signal-to-noise ratio (S/N) and limit of quantitative (LOQ) under 10 times S/N were ..
  27. Wang G, Wu N, He X, Zhang H, Liu J, Wang J. Magnetic graphene dispersive solid phase extraction-ultra performance liquid chromatography tandem mass spectrometry for determination of ?-agonists in urine. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1067:18-24 pubmed publisher
    ..2%-109.4%. Therefore, this method could be used as a simple, rapid, sensitive and accurate tool to determine trace level of ?-agonists in urine...
  28. Iqbal M, Ezzeldin E, Al Rashood K. UPLC-MS/MS assay for identification and quantification of brivaracetam in plasma sample: Application to pharmacokinetic study in rats. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1060:63-70 pubmed publisher
    ..995) having LOD and LLOQ of 0.80 and 1.98ng/mL, respectively. The assay was successfully employed in BVR pharmacokinetic study in rats and can be suitable for therapeutic drug monitoring, pharmacokinetic study and forensic analysis. ..
  29. Storrow A, Lardaro T, Alexander P, Apple F. How low can we go? The high-sensitivity cardiac troponin debate. Ann Emerg Med. 2013;62:580-3 pubmed publisher
  30. Lippi G, Plebani M, Sanchis Gomar F, Banfi G. Current limitations and future perspectives of the Athlete Blood Passport. Eur J Appl Physiol. 2012;112:3693-4 pubmed publisher
  31. Pickering J, Than M, Cullen L, Aldous S, ter Avest E, Body R, et al. Rapid Rule-out of Acute Myocardial Infarction With a Single High-Sensitivity Cardiac Troponin T Measurement Below the Limit of Detection: A Collaborative Meta-analysis. Ann Intern Med. 2017;166:715-724 pubmed publisher
    ..High-sensitivity assays for cardiac troponin T (hs-cTnT) are sometimes used to rapidly rule out acute myocardial infarction (AMI)...
  32. Michi M, Breda M, Belardi J, Dworetzky S, Solazzo L. Development and validation of a highly sensitive gradient chiral separation of pramipexole in human plasma by LC-MS/MS. Bioanalysis. 2017;9:683-692 pubmed publisher
    ..An improved analytical method was developed and validated with a final LLQ for (S)-PPX of 0.1 ng/ml in the presence of 2000 ng/ml of (R)-PPX. ..
  33. Kurotani K, Sato M, Yasuda K, Kashima K, Tanaka S, Hayashi T, et al. Even- and odd-chain saturated fatty acids in serum phospholipids are differentially associated with adipokines. PLoS ONE. 2017;12:e0178192 pubmed publisher
    ..048 and 0.02, respectively). Visfatin was positively associated with both even- and odd-chain saturated fatty acids. The results suggest that even- and odd-chain saturated fatty acids are differentially associated with adipokine profile. ..
  34. Lehmann S, Kieliba T, Beike J, Thevis M, Mercer Chalmers Bender K. Determination of 74 new psychoactive substances in serum using automated in-line solid-phase extraction-liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1064:124-138 pubmed publisher
    ..The guidelines were fully achieved for 62 analytes (with a limit of detection (LOD) between 0.2 and 4?g/L), whilst full validation was not feasible for the remaining 12 analytes...
  35. Zhao F, Gao X, Tang Z, Luo X, Wu M, Xu J, et al. Development of a simple multi-residue determination method of 80 veterinary drugs in Oplegnathus punctatus by liquid chromatography coupled to quadrupole Orbitrap mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1065-1066:20-28 pubmed publisher
    ..91-1001.13ug/kg and 3.52-1002.26ug/kg, respectively. This validated method has been successfully applied on the determination of veterinary drugs in real commercial oplegnathus punctatus samples. ..
  36. Abud J, Luque E, Ramos J, Rodriguez H. Production of monoclonal antibodies and development of a quantitative immuno-polymerase chain reaction assay to detect and quantify recombinant Glutathione S-transferase. Protein Expr Purif. 2017;135:16-23 pubmed publisher
    ..rationally selected as capture and detection antibodies, allowing the development of a sandwich ELISA with a limit of detection (LOD) of 0.01 ?g/ml...
  37. Zhang D, Park J, Abd El Aty A, Kim S, Cho S, Zheng W, et al. Analysis of toldimfos in porcine muscle and bovine milk using liquid chromatography-triple quadrupole mass spectrometry. Biomed Chromatogr. 2017;31: pubmed publisher
    ..This method can be applied for the routine analysis of toldimfos in foods of animal origins. ..
  38. Allen S, Rednour S, Shepard S, Pond B. A simple and sensitive high-performance liquid chromatography-electrochemical detection assay for the quantitative determination of monoamines and respective metabolites in six discrete brain regions of mice. Biomed Chromatogr. 2017;31: pubmed publisher
    ..The described method utilized minimal sample preparation procedures and was optimized to provide the sensitivity limits required for simultaneous monoamine and metabolite analysis in small, discrete brain tissue samples. ..
  39. Tian Q, Rogness J, Meng M, Li Z. Quantitative determination of a siRNA (AD00370) in rat plasma using peptide nucleic acid probe and HPLC with fluorescence detection. Bioanalysis. 2017;9:861-872 pubmed publisher
  40. Kim H, Kosinski P, Kung C, Dang L, Chen Y, Yang H, et al. A fit-for-purpose LC-MS/MS method for the simultaneous quantitation of ATP and 2,3-DPG in human K2EDTA whole blood. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1061-1062:89-96 pubmed publisher
    ..The assay has been successfully applied to K2EDTA human whole blood samples to support clinical studies. ..
  41. Peng H, Hu B, Liu Q, Yang Z, Lu X, Huang R, et al. Liquid chromatography combined with atomic and molecular mass spectrometry for speciation of arsenic in chicken liver. J Chromatogr A. 2014;1370:40-9 pubmed
    ..The identification and quantitation of trace arsenic species present in complex samples demonstrate the potential of HPLC separation with simultaneous ICPMS and ESIMS detection for other speciation applications. ..
  42. van Reenen M, Westerhuis J, Reinecke C, Venter J. Metabolomics variable selection and classification in the presence of observations below the detection limit using an extension of ERp. BMC Bioinformatics. 2017;18:83 pubmed publisher
    ..XERp uses simple rules to classify new subjects and a weight pair to adjust for unequal sample sizes or sensitivity and specificity requirements. ..
  43. Asfaw A, Wolfs K, Schepdael A, Adams E. Thermal desorption-Gas chromatographic methodology for the determination of residual solvents in mesoporous silica. J Chromatogr A. 2017;1500:160-166 pubmed publisher
    ..The results obtained from both procedures were not statistically different (p>0.05) when applied to actual experimental drug samples consisting of itraconazole loaded on MPSi. ..
  44. Ramanujam N, Sivaselvakumar M, Ramalingam S. Fast and parallel determination of PCB 77 and PCB 180 in plasma using ultra performance liquid chromatography with diode array detection: A pharmacokinetic study in Swiss albino mouse. Biomed Chromatogr. 2017;31: pubmed publisher
    ..64%, and the accuracy ranged from 98.90 to 102.33% in mouse plasma. The validated UPLC method was successfully applied to the pharmacokinetic study of PCBs 77 and 180 in mouse plasma. ..
  45. Patel H, Soni K, Trivedi R, Heading H, Geue J, Kansagra K, et al. A sensitive assay for ZYAN1 in human whole blood and urine utilizing positive LC-MS/MS electrospray ionization. Bioanalysis. 2017;9:719-732 pubmed publisher
    ..Interday and intraday accuracy and precision was found well within the acceptance criteria. The validated assay was applied for clinical pharmacokinetics of ZYAN1 in healthy volunteers. ..
  46. Motta E, da Costa J, Bastos J. A validated HPLC-UV method for the analysis of galloylquinic acid derivatives and flavonoids in Copaifera langsdorffii leaves. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1061-1062:240-247 pubmed publisher
    ..18%. The method is reliable for the quality control of C. langsdorffii raw material, its hydroethanolic extract, and could potentially be used to quantify these compounds in other Copaifera species. ..
  47. Mu J, Andersen J, Valenzuela J, Wellems T. High-Sensitivity Assays for Plasmodium falciparum Infection by Immuno-Polymerase Chain Reaction Detection of PfIDEh and PfLDH Antigens. J Infect Dis. 2017;216:713-722 pubmed publisher
    ..falciparum infection; however, these tests often miss cases of low-level parasitemia, and PfHRP-II tests can give false-negative results when P. falciparum strains do not express this antigen...
  48. Judge C, Galvin S, Burke L, Thomas T, Humphreys H, Fitzgerald Hughes D. Search and you will find: detecting extended-spectrum ?-lactamase-producing Klebsiella pneumoniae from a patient's immediate environment. Infect Control Hosp Epidemiol. 2013;34:534-6 pubmed publisher
  49. Amulen D, Spanoghe P, Houbraken M, Tamale A, de Graaf D, Cross P, et al. Environmental contaminants of honeybee products in Uganda detected using LC-MS/MS and GC-ECD. PLoS ONE. 2017;12:e0178546 pubmed publisher
    ..Interestingly, the "zero" detection rate of pesticides in the Mid-Northern zone is a significant indicator of the large potential to promote Ugandan organic honey for the export market. ..
  50. Wang Z, Wang H, Hu P, Jiang J, Zhao Q. A UPLC-MS/MS method for quantification of 5?-androst-3?,5,6?-triol in human plasma: development, validation and its application in clinical pharmacokinetic study. Bioanalysis. 2017;9:873-885 pubmed publisher
    ..A simple method was successfully validated over a concentration range of 2.00-500 ng/ml for 5?-androst-3?,5,6?-triol and applied to investigate its plasma pharmacokinetic profiles in healthy Chinese subjects. ..
  51. Zarei K, Khodadadi A. Very sensitive electrochemical determination of diuron on glassy carbon electrode modified with reduced graphene oxide-gold nanoparticle-Nafion composite film. Ecotoxicol Environ Saf. 2017;144:171-177 pubmed publisher
    ..The reliability of the proposed sensor was confirmed after comparing the results with those obtained using high performance liquid chromatography (HPLC) as a comparative method. ..
  52. Jeon J, Kim H, Jeong I, Hong S, Oh M, Park K, et al. Determination of chlorogenic acids and caffeine in homemade brewed coffee prepared under various conditions. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1064:115-123 pubmed publisher
    ..It was reported that medium-roasted, fine-ground coffees brewed using three pours of boiling water were the healthiest coffee with fluent CGAs. ..
  53. Cuervo D, Loli C, Fernandez Alvarez M, Muñoz G, Carreras D. Determination of doping peptides via solid-phase microelution and accurate-mass quadrupole time-of-flight LC-MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1065-1066:134-144 pubmed publisher
    ..The methodology was validated in terms of selectivity, recovery, matrix effect, precision, sensitivity (limit of detection, LOD), cross contamination, carryover, robustness and stability...
  54. Peters J, van Dam R, van Doorn R, Katerere D, Berthiller F, Haasnoot W, et al. Mycotoxin profiling of 1000 beer samples with a special focus on craft beer. PLoS ONE. 2017;12:e0185887 pubmed publisher
    ..Exceeding the TDI, may have a health impact. A better control of brewing malts for craft beer, should be put in place to circumvent this potential problem...
  55. Brigante T, Miranda L, de Souza I, Acquaro Junior V, Queiroz M. Pipette tip dummy molecularly imprinted solid-phase extraction of Bisphenol A from urine samples and analysis by gas chromatography coupled to mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1067:25-33 pubmed publisher
    ..The developed method was linear from 50 to 500ngmL-1. Precision values had coefficient of variation (CV) ranging from 4 to 14%. The accuracy relative standard deviation values (RSD) varied from -13.6 to 12.3%...
  56. Bahrani S, Ghaedi M, Dashtian K, Ostovan A, Mansoorkhani M, Salehi A. MOF-5(Zn)-Fe2O4 nanocomposite based magnetic solid-phase microextraction followed by HPLC-UV for efficient enrichment of colchicine in root of colchicium extracts and plasma samples. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1067:45-52 pubmed publisher
    ..13ngmL-1) and R2=0.9971. Finally, the UAD-MSPME-HPLC-UV method was successfully applied for determination of colchicine autumn and spring root of colchicium extracts and plasma samples...
  57. Yaren O, Alto B, Gangodkar P, Ranade S, Patil K, Bradley K, et al. Point of sampling detection of Zika virus within a multiplexed kit capable of detecting dengue and chikungunya. BMC Infect Dis. 2017;17:293 pubmed publisher
    ..The assay output is read in ca. 30 min by visualizing (human eye) three-color coded fluorescence signals. Assay in dried format allows it to be run in low-resource environments. ..
  58. Greer B, Maul R, Campbell K, Elliott C. Detection of freshwater cyanotoxins and measurement of masked microcystins in tilapia from Southeast Asian aquaculture farms. Anal Bioanal Chem. 2017;409:4057-4069 pubmed publisher
    ..Graphical abstract á…Ÿ. ..
  59. Feliu C, Cazaubon Y, Guillemin H, Vautier D, Oget O, Millart H, et al. Therapeutic drug monitoring of mitotane: Analytical assay and patient follow-up. Biomed Chromatogr. 2017;31: pubmed publisher
    ..Mitotane retention time was 7.1?min. The limit of detection was 0.1?mg L-1 and the limit of quantification was 0.78?mg L-1 ...
  60. Callol Sanchez L, Munoz Lucas M, Gómez Martín O, Maldonado Sanz J, Civera Tejuca C, Gutiérrez Ortega C, et al. Observation of nonanoic acid and aldehydes in exhaled breath of patients with lung cancer. J Breath Res. 2017;11:026004 pubmed publisher
    ..It is 2.5 times and almost 9 times more likely to be found in the LC group than in the control group or COPD group, respectively. It is independent of histology but depends on tumour stage. ..
  61. Li Y, Hoffmann M, Severin P. Determination of naloxegol in human biological matrices. Bioanalysis. 2017;9:609-619 pubmed publisher
    ..Methods for quantifying naloxegol in human biological matrices have been successfully validated. ..
  62. Matos Cordeiro Borges M, Leijoto de Oliveira H, Bastos Borges K. Molecularly imprinted solid-phase extraction coupled with LC-APCI-MS-MS for the selective determination of serum cholesterol. Electrophoresis. 2017;38:2150-2159 pubmed publisher
    ..46% to 11.54%). The organic MIP showed superior performance compared with the hybrid. Finally, the MISPE procedure followed by LC-APCI-MS-MS analysis showed their suitable applicability to examine CHO and LATHO in serum samples. ..
  63. Hielscher J, Monien B, Abraham K, Jessel S, Seidel A, Lampen A. An isotope-dilution UPLC-MS/MS technique for the human biomonitoring of the internal exposure to glycidol via a valine adduct at the N-terminus of hemoglobin. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1059:7-13 pubmed publisher
    ..The limit of detection was 4 fmol DHP-Val-FTH per injection corresponding to 0.7pmol 2,3-diHOPr-Val/g hemoglobin...
  64. Grabowska Polanowska B, Skowron M, Miarka P, Pietrzycka A, Sliwka I. The application of chromatographic breath analysis in the search of volatile biomarkers of chronic kidney disease and coexisting type 2 diabetes mellitus. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1060:103-110 pubmed publisher
    ..74; p=0.00001) as well as with aspartate aminotransferase (AST), (r=0.61; p=0.001). The presented results suggest the possibility of applying TMA, MeSH, and DMS detection in breath as diagnostic methods. ..
  65. Moreno González D, Hamed A, Gilbert López B, Gámiz Gracia L, García Campaña A. Evaluation of a multiresidue capillary electrophoresis-quadrupole-time-of-flight mass spectrometry method for the determination of antibiotics in milk samples. J Chromatogr A. 2017;1510:100-107 pubmed publisher
    ..Thus, the proposed SPE-CZE-Q-TOF-MS method is suitable for multiclass multiresidue monitoring in different types of milk samples. ..
  66. Georgiou C, McNae I, Wear M, Ioannidis H, Michel J, Walkinshaw M. Pushing the Limits of Detection of Weak Binding Using Fragment-Based Drug Discovery: Identification of New Cyclophilin Binders. J Mol Biol. 2017;429:2556-2570 pubmed publisher
    ..A detailed comparison of the merits and drawbacks of the experimental and computational techniques is presented, and emerging strategies for designing ligands with enhanced isoform specificity are described. ..
  67. Monteiro S, Santos R. Nanofluidic digital PCR for the quantification of Norovirus for water quality assessment. PLoS ONE. 2017;12:e0179985 pubmed publisher
    ..This study proved the usefulness of the newly developed RT-dPCR protocol for a sensitive and accurate detection of low-copy targets. ..
  68. Wang J, Jiang N, Cai Z, Li W, Li J, Lin X, et al. Sodium hyaluronate-functionalized urea-formaldehyde monolithic column for hydrophilic in-tube solid-phase microextraction of melamine. J Chromatogr A. 2017;1515:54-61 pubmed publisher
    ..Under the optimized SPME conditions, the limit of detection (LOD) of MEL was found to be 0...
  69. Zeng Z, Ji Z, Hu N, Bai B, Wang H, Suo Y. A sensitive pre-column derivatization method for the analysis of free fatty acids by RP-HPLC with fluorescence detector and its application to Caragana species. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1064:151-159 pubmed publisher
    ..The total content of FFAs in all parts of CK is higher than that of CM. The distribution of FFAs in plants is obviously different even in the congeneric among different species. ..
  70. Li H, Ai L, Fan S, Wang Y, Sun D. Rapid determination of 18 glucocorticoids in serum using reusable on-line SPE polymeric monolithic column coupled with LC-quadrupole/orbitrap high-resolution mass spectrometer. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1065-1066:79-86 pubmed publisher
    ..The method was used to analyze the dynamic contents variation of cortisone and hydrocortisone in serum before and after the surgery. ..
  71. Mudge E, Murch S, Brown P. Leaner and greener analysis of cannabinoids. Anal Bioanal Chem. 2017;409:3153-3163 pubmed publisher
    ..validation determined the linearity, selectivity, accuracy, repeatability, intermediate precision, limit of detection, and limit of quantitation of the method...
  72. Tabani H, Asadi S, Nojavan S, Parsa M. Introduction of agarose gel as a green membrane in electromembrane extraction: An efficient procedure for the extraction of basic drugs with a wide range of polarities. J Chromatogr A. 2017;1497:47-55 pubmed publisher
    ..5-1.8ngmL-1 and 5.0-6.0ngmL-1, respectively. Finally, the proposed method was successfully applied to determine concentrations of the model drugs in the wastewater sample. ..
  73. Howe H, Thong B, Kong K, Chng H, Lian T, Chia F, et al. Associations of B cell-activating factor (BAFF) and anti-BAFF autoantibodies with disease activity in multi-ethnic Asian systemic lupus erythematosus patients in Singapore. Clin Exp Immunol. 2017;189:298-303 pubmed publisher
  74. Vincent U, Serano F, von Holst C. Development and validation of a multi-analyte method for the regulatory control of carotenoids used as feed additives in fish and poultry feed. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2017;34:1285-1297 pubmed publisher
    ..6% to 15%. Based on the acceptable performance obtained in the validation study, the multi-analyte method is considered fit for the intended purpose. ..
  75. Qiu Z, Yu J, Dai Y, Yang Y, Lu X, Xu J, et al. A simple LC-MS/MS method facilitated by salting-out assisted liquid-liquid extraction to simultaneously determine trans-resveratrol and its glucuronide and sulfate conjugates in rat plasma and its application to pharmacokinetic assay. Biomed Chromatogr. 2017;31: pubmed publisher
    ..4%. The reproducible SALLE method simplified the sample preparation, drastically improved the accuracy of the concomitant assay and gave full consideration of extraction recovery to each target analyte in bio-samples. ..
  76. Wang B, Park B, Xu B, Kwon Y. Label-free biosensing of Salmonella enterica serovars at single-cell level. J Nanobiotechnology. 2017;15:40 pubmed publisher
    ..The limit of detection (LOD) was determined as 3 × 104 CFU mL-1...
  77. Ran F, Liu H, Wang X, Guo Y. A novel molybdenum disulfide nanosheet self-assembled flower-like monolithic sorbent for solid-phase extraction with high efficiency and long service life. J Chromatogr A. 2017;1507:18-24 pubmed publisher
    ..Finally, the SPE-HPLC-VWD method using the as-prepared sorbent was applied to flavonoid analysis in beverage samples with satisfactory results. ..
  78. Imran M, Fazal E Habib -, Tawab A, Rauf W, Rahman M, Khan Q, et al. LC-MS/MS based method development for the analysis of florfenicol and its application to estimate relative distribution in various tissues of broiler chicken. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1063:163-173 pubmed publisher
    ..4% and the precision values for within day and between days ranged from 1.2-11.7%, respectively. Limit of detection (LOD), limit of quantification (LOD), CC? and CC? values were 0.98, 3.2, 113 and 126?g/kg, respectively...
  79. Kang S, Ahn S, Lee S, Jeong J, Sung J, Oh Y, et al. Shifted termination assay (STA) fragment analysis to detect BRAF V600 mutations in papillary thyroid carcinomas. Diagn Pathol. 2013;8:121 pubmed publisher
    ..Serial dilutions of BRAF mutant cell line (SNU-790) were used to calculate limit of detection (LOD). BRAF mutations were detected in 119 (74.8%) PTCs by STA fragment analysis...
  80. Jarolim P. High sensitivity cardiac troponin assays in the clinical laboratories. Clin Chem Lab Med. 2015;53:635-52 pubmed publisher
    ..We envisage that the high sensitivity assays will become important tools for predicting each patient's risk of future adverse events and for guiding and monitoring corresponding adjustments of preventative therapeutic interventions. ..
  81. Jarolim P. Terminology of cardiac troponin assays and data censoring. Clin Chem Lab Med. 2017;55:1672-1674 pubmed publisher
    ..In addition, the need for reasonable censoring of clinical laboratory test results is contrasted with potential loss of valuable clinical information. ..
  82. Hao M, Zhang P, Li B, Liu X, Zhao Y, Tan H, et al. Development and evaluation of an up-converting phosphor technology-based lateral flow assay for the rapid, simultaneous detection of Vibrio cholerae serogroups O1 and O139. PLoS ONE. 2017;12:e0179937 pubmed publisher
    ..Therefore, successful development of Vch-UPT-LF as a dual-target assay for quantitative detection makes this assay a good candidate POCT method for the detection and surveillance of epidemic cholera. ..
  83. Zheng N, Wang X, Wang Y, Xu G, Zhang H, Dai W, et al. A sensitive liquid chromatography/electrospray tandem mass spectroscopy method for simultaneous quantification of a disulfide bond doxorubicin conjugation prodrug and activated doxorubicin: Application to cellular pharmacokinetic and catabolism studi. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1065-1066:96-103 pubmed publisher
    ..The assay was successfully used in the cellular metabolism and pharmacokinetics study of DOX-S-S-DOX and it may give a clue to explore analytical methods of other prodrug forms of DOX. ..
  84. Dinse G, Jusko T, Ho L, Annam K, Graubard B, Hertz Picciotto I, et al. Accommodating measurements below a limit of detection: a novel application of Cox regression. Am J Epidemiol. 2014;179:1018-24 pubmed publisher
    In environmental epidemiology, measurements of exposure biomarkers often fall below the assay's limit of detection. Existing methods for handling this problem, including deletion, substitution, parametric regression, and multiple ..
  85. Tan X, Lian K, Li X, Li N, Wang W, Kang W, et al. Development of a derivatization method for the quantification of hydrogen sulfide and its application in vascular calcification rats. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1055-1056:8-14 pubmed publisher
    ..1% (v/v) formic acid-acetonitrile. The limit of detection for the derivatized product was 0.03nmol/mL...
  86. Zokaei M, Abedi A, Kamankesh M, Shojaee Aliababadi S, Mohammadi A. Ultrasonic-assisted extraction and dispersive liquid-liquid microextraction combined with gas chromatography-mass spectrometry as an efficient and sensitive method for determining of acrylamide in potato chips samples. Food Chem. 2017;234:55-61 pubmed publisher
    ..The relative standard deviation (RSD) for the seven analyses was 6.8%. The limit of detection (LOD) and limit of quantification (LOQ) were 0.6ngg-1 and 2ngg-1, respectively...
  87. Wang K, Lin K, Huang X, Chen M. A Simple and Fast Extraction Method for the Determination of Multiclass Antibiotics in Eggs Using LC-MS/MS. J Agric Food Chem. 2017;65:5064-5073 pubmed publisher
    ..A total of five antibiotics were successfully detected in 22 commercial eggs from local markets. This work suggests that the method is suitable for the analysis of multiclass antibiotics in eggs. ..
  88. Huang S, Gan N, Liu H, Zhou Y, Chen Y, Cao Y. Simultaneous and specific enrichment of several amphenicol antibiotics residues in food based on novel aptamer functionalized magnetic adsorbents using HPLC-DAD. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1060:247-254 pubmed publisher
    ..It is a universal platform which can be extended to selective enrichment other organic pollutants residues if changing the modified aptamers. ..
  89. Suh J, Makarova A, Gomez J, Paul L, Saba J. An LC/MS/MS method for quantitation of chemopreventive sphingadienes in food products and biological samples. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1061-1062:292-299 pubmed publisher
    ..SD uptake in the gut was maximal in the duodenum and peaked 1h after gavage delivery. Disappearance of SDs in the lower gastrointestinal tract suggests either rapid metabolism to yet unidentified products or potentially luminal export. ..
  90. Gras R, Luong J, Shellie R. Gas chromatography with diode array detection in series with flame ionisation detection. J Chromatogr A. 2017;1500:153-159 pubmed publisher
  91. Punt A, Langenhorst J, Egas A, Boelens J, Van Kesteren C, van Maarseveen E. Simultaneous quantification of busulfan, clofarabine and F-ARA-A using isotope labelled standards and standard addition in plasma by LC-MS/MS for exposure monitoring in hematopoietic cell transplantation conditioning. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1055-1056:81-85 pubmed publisher
    ..In addition, a robust and cost-effective method to correct for matrix interference by standard addition was established. ..
  92. Sun L, Kong D, Gu W, Guo X, Tao W, Shan Z, et al. Determination of glyphosate in soil/sludge by high performance liquid chromatography. J Chromatogr A. 2017;1502:8-13 pubmed publisher
    ..999) was observed within the range of 0.005-0.5mg/L. The limit of detection (LOD) and the limit of quantitation (LOQ) were determined to be 0.01mg/kg and 0.04mg/kg, respectively...