dynamin i


Summary: A subtype of dynamin found primarily in the NEURONS of the brain.

Top Publications

  1. Cnops L, Hu T, Vanden Broeck J, Burnat K, Van den Bergh G, Arckens L. Age- and experience-dependent expression of dynamin I and synaptotagmin I in cat visual system. J Comp Neurol. 2007;504:254-64 pubmed publisher
    b>Dynamin I (Dyn I) and Synaptotagmin I (Syt I) are essential for endocytosis-exocytosis processes, thus for neurotransmission...
  2. Chyung J, Selkoe D. Inhibition of receptor-mediated endocytosis demonstrates generation of amyloid beta-protein at the cell surface. J Biol Chem. 2003;278:51035-43 pubmed
    ..Here, we used HeLa cells stably expressing a tetracycline-regulated dominant-negative dynamin I (dyn K44A), which selectively inhibits receptor-mediated endocytosis, and analyzed the effects on the processing ..
  3. Kelly B, Ferreira A. beta-Amyloid-induced dynamin 1 degradation is mediated by N-methyl-D-aspartate receptors in hippocampal neurons. J Biol Chem. 2006;281:28079-89 pubmed
    ..These data also highlight the calpain system as a novel therapeutic target for early stage AD intervention. ..
  4. Tsuboi T, McMahon H, Rutter G. Mechanisms of dense core vesicle recapture following "kiss and run" ("cavicapture") exocytosis in insulin-secreting cells. J Biol Chem. 2004;279:47115-24 pubmed
  5. Sundborger A, Soderblom C, Vorontsova O, Evergren E, Hinshaw J, Shupliakov O. An endophilin-dynamin complex promotes budding of clathrin-coated vesicles during synaptic vesicle recycling. J Cell Sci. 2011;124:133-43 pubmed publisher
    ..Therefore, endophilin recruits dynamin to a restricted part of the CCP neck, forming a complex, which promotes budding of new synaptic vesicles. ..
  6. Fulop T, Doreian B, Smith C. Dynamin I plays dual roles in the activity-dependent shift in exocytic mode in mouse adrenal chromaffin cells. Arch Biochem Biophys. 2008;477:146-54 pubmed publisher
    ..We report that disruption of dynamin I function alters both fusion modes...
  7. Faelber K, Posor Y, Gao S, Held M, Roske Y, Schulze D, et al. Crystal structure of nucleotide-free dynamin. Nature. 2011;477:556-60 pubmed publisher
    ..This intricate domain interaction rationalizes a number of disease-related mutations in dynamin 2 and suggests a structural model for the mechanochemical coupling that reconciles previous models of dynamin function. ..
  8. Bauerfeind R, Takei K, De Camilli P. Amphiphysin I is associated with coated endocytic intermediates and undergoes stimulation-dependent dephosphorylation in nerve terminals. J Biol Chem. 1997;272:30984-92 pubmed
    ..an abundant presynaptic protein that interacts via its COOH-terminal src homology 3 (SH3) domain with the GTPase dynamin I and the inositol-5-phosphatase synaptojanin...
  9. Grabs D, Slepnev V, Songyang Z, David C, Lynch M, Cantley L, et al. The SH3 domain of amphiphysin binds the proline-rich domain of dynamin at a single site that defines a new SH3 binding consensus sequence. J Biol Chem. 1997;272:13419-25 pubmed
    ..neuronal protein that is highly concentrated in nerve terminals where it interacts via its SH3 domain with dynamin I, a GTPase implicated in synaptic vesicle endocytosis...

More Information


  1. Cao H, Garcia F, McNiven M. Differential distribution of dynamin isoforms in mammalian cells. Mol Biol Cell. 1998;9:2595-609 pubmed
    ..cDNAs encoding the different dynamin isoforms were sequenced and revealed four additional spliced variants for dynamin I and nine for dynamin III...
  2. Gammie A, Kurihara L, Vallee R, Rose M. DNM1, a dynamin-related gene, participates in endosomal trafficking in yeast. J Cell Biol. 1995;130:553-66 pubmed
    ..The protein exhibits 41% overall identity with full-length dynamin I and 55% identity with the most highly conserved 400-amino acid GTPase region...
  3. Cousin M, Tan T, Robinson P. Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin. J Neurochem. 2001;76:105-16 pubmed
    b>Dynamin I and at least five other nerve terminal proteins, amphiphysins I and II, synaptojanin, epsin and eps15 (collectively called dephosphins), are coordinately dephosphorylated by calcineurin during endocytosis of synaptic vesicles...
  4. Solomaha E, Palfrey H. Conformational changes in dynamin on GTP binding and oligomerization reported by intrinsic and extrinsic fluorescence. Biochem J. 2005;391:601-11 pubmed
    ..In addition, energy transfer was observed when oligomerization was promoted in mixtures of IAEDANS.dynamin-2 and 4-(4-dimethylaminophenylazo)benzoic acid-coupled dynamin-2, an effect that was counteracted by GTP but not GDP. ..
  5. Hayashi M, Raimondi A, O TOOLE E, Paradise S, Collesi C, Cremona O, et al. Cell- and stimulus-dependent heterogeneity of synaptic vesicle endocytic recycling mechanisms revealed by studies of dynamin 1-null neurons. Proc Natl Acad Sci U S A. 2008;105:2175-80 pubmed publisher
    ..These findings reveal a striking heterogeneity in the mode of synaptic vesicle recycling in different synapses and functional states. ..
  6. Qualmann B, Kelly R. Syndapin isoforms participate in receptor-mediated endocytosis and actin organization. J Cell Biol. 2000;148:1047-62 pubmed
    ..Certain splice variants of rat SdpII in other species were named FAP52 and PACSIN 2. SdpII binds dynamin I, synaptojanin, synapsin I, and the neural Wiskott-Aldrich syndrome protein (N-WASP), a stimulator of Arp2/3 ..
  7. Jones B, Fangman W. Mitochondrial DNA maintenance in yeast requires a protein containing a region related to the GTP-binding domain of dynamin. Genes Dev. 1992;6:380-9 pubmed
    ..On the basis of the results of this study, we conclude that cells can partition single mitochondrial genomes, and that when a cell receives a single molecule at division it is able to restore the normal complement of multiple copies. ..
  8. Lee E, De Camilli P. Dynamin at actin tails. Proc Natl Acad Sci U S A. 2002;99:161-6 pubmed
    ..At endocytic sites, dynamin may couple the fission reaction to the polymerization of an actin pool that functions in the separation of the endocytic vesicles from the plasma membrane. ..
  9. Chen Hwang M, Chen H, Elzinga M, Hwang Y. Dynamin is a minibrain kinase/dual specificity Yak1-related kinase 1A substrate. J Biol Chem. 2002;277:17597-604 pubmed
    ..The interactions of dynamin with amphiphysin and endophilin are essential for the formation of endocytic complexes; our results suggest that Mnbk/Dyrk1A may function as a regulator controlling the assembly of endocytic apparatus. ..
  10. Chappie J, Acharya S, Liu Y, Leonard M, Pucadyil T, Schmid S. An intramolecular signaling element that modulates dynamin function in vitro and in vivo. Mol Biol Cell. 2009;20:3561-71 pubmed publisher
    ..These data suggest that the components of the GTPase-GED interface act as an intramolecular signaling module, which we term the bundle signaling element, that can modulate dynamin function in vitro and in vivo. ..
  11. Watanabe T, Iwasaki K, Takasaki K, Yamagata N, Fujino M, Nogami A, et al. Dynamin 1 depletion and memory deficits in rats treated with Abeta and cerebral ischemia. J Neurosci Res. 2010;88:1908-17 pubmed publisher
    ..These results suggest that Abeta oligomers induce memory impairment via dynamin 1 degradation, which may imply that dynamin 1 degradation is one of the causes of synaptic dysfunction in AD. ..
  12. Slepnev V, Ochoa G, Butler M, Grabs D, De Camilli P. Role of phosphorylation in regulation of the assembly of endocytic coat complexes. Science. 1998;281:821-4 pubmed
    ..Thus, phosphorylation regulates the association and dissociation cycle of the clathrin-based endocytic machinery, and calcium-dependent dephosphorylation of endocytic proteins could prepare nerve terminals for a burst of endocytosis. ..
  13. Baillat G, Gaillard S, Castets F, Monneron A. Interactions of phocein with nucleoside-diphosphate kinase, Eps15, and Dynamin I. J Biol Chem. 2002;277:18961-6 pubmed
    ..NDPK and Eps15 were recently shown to be functional neighbors of dynamin. Dynamin I is shown here to directly interact with NDPK through its C-terminal proline-rich domain, whereas recombinant ..
  14. Ferguson S, Ferguson S, Raimondi A, Paradise S, Shen H, Mesaki K, et al. Coordinated actions of actin and BAR proteins upstream of dynamin at endocytic clathrin-coated pits. Dev Cell. 2009;17:811-22 pubmed publisher
    ..Our data also demonstrate that the relationship between dynamin and actin is intimately connected to dynamin's endocytic role and that dynamin terminates a powerful actin- and BAR protein-dependent tubulating activity...
  15. Sontag J, Fykse E, Ushkaryov Y, Liu J, Robinson P, Sudhof T. Differential expression and regulation of multiple dynamins. J Biol Chem. 1994;269:4547-54 pubmed
    ..The second site of alternative splicing results in the generation of different C termini in dynamin I and in the inclusion or exclusion of an interior four-amino acid sequence in dynamin II...
  16. Smillie K, Cousin M. Akt/PKB controls the activity-dependent bulk endocytosis of synaptic vesicles. Traffic. 2012;13:1004-11 pubmed publisher
    ..The dephosphorylation of Ser774 on dynamin I is essential for triggering of ADBE, as is its subsequent rephosphorylation by glycogen synthase kinase 3 (GSK3)...
  17. Otomo M, Takahashi K, Miyoshi H, Osada K, Nakashima H, Yamaguchi N. Some selective serotonin reuptake inhibitors inhibit dynamin I guanosine triphosphatase (GTPase). Biol Pharm Bull. 2008;31:1489-95 pubmed
    Neuronal dynamin I plays a critical role in the recycling of synaptic vesicles, and thus in nervous system function...
  18. Tsuboi T, Terakawa S, Scalettar B, Fantus C, Roder J, Jeromin A. Sweeping model of dynamin activity. Visualization of coupling between exocytosis and endocytosis under an evanescent wave microscope with green fluorescent proteins. J Biol Chem. 2002;277:15957-61 pubmed
    ..of these processes by observing the secretory vesicle marker synaptobrevin and the endocytotic vesicle marker dynamin I tagged with green and red fluorescent proteins under an evanescent wave microscope in pheochromocytoma cells...
  19. Artalejo C, Elhamdani A, Palfrey H. Sustained stimulation shifts the mechanism of endocytosis from dynamin-1-dependent rapid endocytosis to clathrin- and dynamin-2-mediated slow endocytosis in chromaffin cells. Proc Natl Acad Sci U S A. 2002;99:6358-63 pubmed
  20. Shinyama H, Masuzaki H, Fang H, Flier J. Regulation of melanocortin-4 receptor signaling: agonist-mediated desensitization and internalization. Endocrinology. 2003;144:1301-14 pubmed
    ..Overexpression of dominant-negative mutants of beta-arrestin1-V53D and dynamin I-K44A prevented agonist-mediated internalization of MC4R...
  21. Sesaki H, Jensen R. Division versus fusion: Dnm1p and Fzo1p antagonistically regulate mitochondrial shape. J Cell Biol. 1999;147:699-706 pubmed
    ..Our results thus suggest that mitochondrial shape is normally controlled by a balance between division and fusion which requires Dnm1p and Fzo1p, respectively. ..
  22. Kim Y, Kim S, Lee S, Kim S, Kim Y, Park Z, et al. Interaction of SPIN90 with dynamin I and its participation in synaptic vesicle endocytosis. J Neurosci. 2005;25:9515-23 pubmed
    ..Here, we found that SPIN90 is expressed in the presynaptic compartment in which it binds dynamin I, a key component of the endocytic machinery, and that it participates in synaptic vesicle endocytosis...
  23. Raimondi A, Ferguson S, Lou X, Armbruster M, Paradise S, Giovedi S, et al. Overlapping role of dynamin isoforms in synaptic vesicle endocytosis. Neuron. 2011;70:1100-14 pubmed publisher
    ..Persistence of synaptic transmission in their absence indicates that if dynamin plays essential functions in neurons, such functions can be achieved by the very low levels of dynamin 2. ..
  24. Kelly B, Vassar R, Ferreira A. Beta-amyloid-induced dynamin 1 depletion in hippocampal neurons. A potential mechanism for early cognitive decline in Alzheimer disease. J Biol Chem. 2005;280:31746-53 pubmed
    ..These data suggest a mechanism to explain the early cognitive loss without a major decline in synapse number observed in AD and propose a novel therapeutic target for AD intervention. ..
  25. Mallozzi C, D Amore C, Camerini S, Macchia G, Crescenzi M, Petrucci T, et al. Phosphorylation and nitration of tyrosine residues affect functional properties of Synaptophysin and Dynamin I, two proteins involved in exo-endocytosis of synaptic vesicles. Biochim Biophys Acta. 2013;1833:110-21 pubmed publisher
    ..SYP forms a complex with Dynamin I (DynI), a GTPase required for SV endocytosis, which may be regulated by tyrosine phosphorylation of SYP...
  26. Anggono V, Robinson P. Syndapin I and endophilin I bind overlapping proline-rich regions of dynamin I: role in synaptic vesicle endocytosis. J Neurochem. 2007;102:931-43 pubmed
    b>Dynamin I mediates vesicle fission during synaptic vesicle endocytosis (SVE). Its proline-rich domain (PRD) binds the Src-homology 3 (SH3) domain of a subset of proteins that can deform membranes...
  27. Desai S, Ashby B. Agonist-induced internalization and mitogen-activated protein kinase activation of the human prostaglandin EP4 receptor. FEBS Lett. 2001;501:156-60 pubmed
    ..Co-expression of dominant negative beta-arrestin (319-418) or dynamin I (K44A) with the EP4 receptor reduced internalization...
  28. Carey R, Balcz B, Lopez Coviella I, Slack B. Inhibition of dynamin-dependent endocytosis increases shedding of the amyloid precursor protein ectodomain and reduces generation of amyloid beta protein. BMC Cell Biol. 2005;6:30 pubmed
    ..Human embryonic kidney cells were transfected with a dominant negative mutant of dynamin I, an important mediator of clathrin-dependent endocytosis, and APP proteolysis was analyzed...
  29. Reubold T, Eschenburg S, Becker A, Leonard M, Schmid S, Vallee R, et al. Crystal structure of the GTPase domain of rat dynamin 1. Proc Natl Acad Sci U S A. 2005;102:13093-8 pubmed
    ..Our results support a model in which switch I residue Arg-59 gates GTP binding in an assembly-dependent manner and the GTPase effector domain functions as an assembly-dependent GTPase activating protein in the fashion of RGS-type GAPs. ..
  30. Barylko B, Wang L, Binns D, Ross J, Tassin T, Collins K, et al. The proline/arginine-rich domain is a major determinant of dynamin self-activation. Biochemistry. 2010;49:10592-4 pubmed publisher
    ..Dynamin proline/arginine-rich domains also mediate interactions with SH3 domain-containing proteins and thus regulate both self-association and heteroassociation of dynamins. ..
  31. Jiang S, Avraham H, Kim T, Rogers R, Avraham S. Receptor-type PTP-NP inhibition of Dynamin-1 GTPase activity is associated with neuronal depolarization. Cell Signal. 2006;18:1439-46 pubmed
    ..This is the first report to suggest a novel function of this unique receptor-type tyrosine phosphatase as a potential regulator of Dynamin-1 GTPase activity upon neuronal depolarization. ..
  32. Patterson E, Minor K, Tchernatynskaia A, Taylor S, Shelton G, Ekenstedt K, et al. A canine DNM1 mutation is highly associated with the syndrome of exercise-induced collapse. Nat Genet. 2008;40:1235-9 pubmed publisher
  33. Clayton E, Cousin M. The molecular physiology of activity-dependent bulk endocytosis of synaptic vesicles. J Neurochem. 2009;111:901-14 pubmed publisher
    ..It is becoming clear that an understanding of the molecular physiology of ADBE will be of critical importance in attempts to modulate both normal and abnormal synaptic function during intense neuronal activity. ..
  34. Ramachandran R, Schmid S. Real-time detection reveals that effectors couple dynamin's GTP-dependent conformational changes to the membrane. EMBO J. 2008;27:27-37 pubmed
    ..Amphiphysin I has the opposite effect. Thus, dynamin's mechanochemical properties on a membrane surface are dynamically regulated by its GTPase cycle and major binding partners. ..
  35. Kitzmueller E, Krapfenbauer K, Hoeger H, Weitzdoerfer R, Lubec G, Lubec B. Life-long effects of perinatal asphyxia on stress-induced proteins and dynamin 1 in rat brain. Neurochem Res. 2004;29:1767-77 pubmed
    ..Decreased dynamin-1 levels may be considered as a long-term effect on the exocytotic system possibly reflecting or leading to impaired neuronal transport and vesicle-trafficking in PA of the rat of advanced age. ..
  36. Anggono V, Smillie K, Graham M, Valova V, Cousin M, Robinson P. Syndapin I is the phosphorylation-regulated dynamin I partner in synaptic vesicle endocytosis. Nat Neurosci. 2006;9:752-60 pubmed
    b>Dynamin I is dephosphorylated at Ser-774 and Ser-778 during synaptic vesicle endocytosis (SVE) in nerve terminals. Phosphorylation was proposed to regulate the assembly of an endocytic protein complex with amphiphysin or endophilin...
  37. Tan T, Valova V, Malladi C, Graham M, Berven L, Jupp O, et al. Cdk5 is essential for synaptic vesicle endocytosis. Nat Cell Biol. 2003;5:701-10 pubmed
    ..Here, we show that cyclin-dependent kinase 5 (Cdk5) phosphorylates dynamin I on Ser 774 and Ser 778 in vitro, which are identical to its endogenous phosphorylation sites in vivo...
  38. Suginta W, Karoulias N, Aitken A, Ashley R. Chloride intracellular channel protein CLIC4 (p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms. Biochem J. 2001;359:55-64 pubmed
    ..Brain CLIC4 binds dynamin I, alpha-tubulin, beta-actin, creatine kinase and two 14-3-3 isoforms; the interactions are confirmed in vivo by ..
  39. Nakata T, Takemura R, Hirokawa N. A novel member of the dynamin family of GTP-binding proteins is expressed specifically in the testis. J Cell Sci. 1993;105 ( Pt 1):1-5 pubmed
    ..Our data imply that a number of dynamin family proteins, which are products of distinct genes, may play different roles specific to each cell type in the same rat. ..
  40. Huang Y, Chen Hwang M, Dolios G, Murakami N, Padovan J, Wang R, et al. Mnb/Dyrk1A phosphorylation regulates the interaction of dynamin 1 with SH3 domain-containing proteins. Biochemistry. 2004;43:10173-85 pubmed
    ..Since S857 is unique to dynamin 1xa isoforms, Mnb/Dyrk1A regulation of dynamin 1 is expected to be specific to these spliced variants. ..
  41. Shin H, Shinotsuka C, Torii S, Murakami K, Nakayama K. Identification and subcellular localization of a novel mammalian dynamin-related protein homologous to yeast Vps1p and Dnm1p. J Biochem. 1997;122:525-30 pubmed
    ..These data suggest that DVLP is not involved in the formation of known coated vesicles. ..
  42. Graham M, Anggono V, Bache N, Larsen M, Craft G, Robinson P. The in vivo phosphorylation sites of rat brain dynamin I. J Biol Chem. 2007;282:14695-707 pubmed
    b>Dynamin I (dynI) is phosphorylated in synaptosomes at Ser(774) and Ser(778) by cyclin-dependent kinase 5 to regulate recruitment of syndapin I for synaptic vesicle endocytosis, and in PC12 cells on Ser(857)...
  43. Kasai K, Shin H, Shinotsuka C, Murakami K, Nakayama K. Dynamin II is involved in endocytosis but not in the formation of transport vesicles from the trans-Golgi network. J Biochem. 1999;125:780-9 pubmed
    ..There are three dynamin genes in mammals: dynamin I is neuron-specific, dynamin II shows ubiquitous expression, and dynamin III is expressed in testis, brain, and ..
  44. Witke W, Podtelejnikov A, Di Nardo A, Sutherland J, Gurniak C, Dotti C, et al. In mouse brain profilin I and profilin II associate with regulators of the endocytic pathway and actin assembly. EMBO J. 1998;17:967-76 pubmed
    ..we characterized a number of ligands for profilin I and profilin II from mouse brain extracts including dynamin I, clathrin, synapsin, Rho-associated coiled-coil kinase, the Rac-associated protein NAP1 and a member of the NSF/..
  45. Odell L, Howan D, Gordon C, Robertson M, Chau N, Mariana A, et al. The pthaladyns: GTP competitive inhibitors of dynamin I and II GTPase derived from virtual screening. J Med Chem. 2010;53:5267-80 pubmed publisher
    We report the development of a homology model for the GTP binding domain of human dynamin I based on the corresponding crystal structure of Dictyostelium discoidum dynamin A...
  46. Gray N, Fourgeaud L, Huang B, Chen J, Cao H, Oswald B, et al. Dynamin 3 is a component of the postsynapse, where it interacts with mGluR5 and Homer. Curr Biol. 2003;13:510-5 pubmed
    ..These data suggest that Dyn3 is a postsynaptic dynamin and, like its binding partner Homer, plays a significant role in dendritic spine morphogenesis and remodeling. ..
  47. Evergren E, Tomilin N, Vasylieva E, Sergeeva V, Bloom O, Gad H, et al. A pre-embedding immunogold approach for detection of synaptic endocytic proteins in situ. J Neurosci Methods. 2004;135:169-74 pubmed
    ..Thus, our experiments show that the current pre-embedding immunogold method is a useful experimental tool to study the molecular mechanisms of synaptic vesicle recycling. ..
  48. Shen H, Ferguson S, Dephoure N, Park R, Yang Y, Volpicelli Daley L, et al. Constitutive activated Cdc42-associated kinase (Ack) phosphorylation at arrested endocytic clathrin-coated pits of cells that lack dynamin. Mol Biol Cell. 2011;22:493-502 pubmed publisher
    ..These findings reveal a link between progression of clathrin-coated pits to endocytic vesicles and an activation-deactivation cycle of Ack. ..
  49. Liu Y, Surka M, Schroeter T, Lukiyanchuk V, Schmid S. Isoform and splice-variant specific functions of dynamin-2 revealed by analysis of conditional knock-out cells. Mol Biol Cell. 2008;19:5347-59 pubmed publisher
    ..This splice variant specificity correlated with their differential targeting to the Golgi. These studies reveal isoform and splice-variant specific functions for Dyn2. ..
  50. Yamashita T, Hige T, Takahashi T. Vesicle endocytosis requires dynamin-dependent GTP hydrolysis at a fast CNS synapse. Science. 2005;307:124-7 pubmed
    ..Thus, the guanosine triphosphatase dynamin-1 is indispensable for vesicle endocytosis at this fast central nervous system (CNS) synapse. ..
  51. Obar R, Collins C, Hammarback J, Shpetner H, Vallee R. Molecular cloning of the microtubule-associated mechanochemical enzyme dynamin reveals homology with a new family of GTP-binding proteins. Nature. 1990;347:256-61 pubmed
    ..These relationships imply the existence of a new family of GTPases with physiological roles that may include microtubule-based motility and protein sorting. ..
  52. Smirnova E, Shurland D, Ryazantsev S, van der Bliek A. A human dynamin-related protein controls the distribution of mitochondria. J Cell Biol. 1998;143:351-8 pubmed
    ..The function of this new dynamin-related protein in organelle morphology represents a novel role for a member of the dynamin family of proteins. ..
  53. McMahon H, Wigge P, Smith C. Clathrin interacts specifically with amphiphysin and is displaced by dynamin. FEBS Lett. 1997;413:319-22 pubmed
    ..As well as interacting with dynamin I, the full-length protein bound to a weaker 180-kDa band. Immunoblotting demonstrated this protein to be clathrin...