neuronal wiskott aldrich syndrome protein


Summary: A member of the Wiskott-Aldrich syndrome protein family that is found at high levels in NERVE CELLS. It interacts with GRB2 ADAPTOR PROTEIN and with CDC42 PROTEIN.

Top Publications

  1. Sallee N, Rivera G, Dueber J, Vasilescu D, Mullins R, Mayer B, et al. The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. Nature. 2008;454:1005-8 pubmed publisher
    ..Thus, this pathogen has used a simple autoinhibitory fragment as a component to build a highly effective actin polymerization machine. ..
  2. DesMarais V, Yamaguchi H, Oser M, Soon L, Mouneimne G, Sarmiento C, et al. N-WASP and cortactin are involved in invadopodium-dependent chemotaxis to EGF in breast tumor cells. Cell Motil Cytoskeleton. 2009;66:303-16 pubmed publisher
  3. Bu W, Chou A, Lim K, Sudhaharan T, Ahmed S. The Toca-1-N-WASP complex links filopodial formation to endocytosis. J Biol Chem. 2009;284:11622-36 pubmed publisher
    ..Taken together, these data suggest that the Toca-1-N-WASP complex can link filopodial formation to endocytosis. ..
  4. Dharmalingam E, Haeckel A, Pinyol R, Schwintzer L, Koch D, Kessels M, et al. F-BAR proteins of the syndapin family shape the plasma membrane and are crucial for neuromorphogenesis. J Neurosci. 2009;29:13315-27 pubmed publisher
    ..Our data thus reveal that syndapin I is crucial for neuromorphogenesis and that different N-WASP activators ensure fine control of N-WASP activity and have distinct functions during neuronal network formation. ..
  5. Park H, Cox D. Cdc42 regulates Fc gamma receptor-mediated phagocytosis through the activation and phosphorylation of Wiskott-Aldrich syndrome protein (WASP) and neural-WASP. Mol Biol Cell. 2009;20:4500-8 pubmed publisher
    ..These results indicate that Cdc42 is essential for the activation of WASP and N-WASP, leading to actin assembly and phagocytic cup formation by macrophages during Fc(gamma)R-mediated phagocytosis. ..
  6. Hänisch J, Ehinger J, Ladwein M, Rohde M, Derivery E, Bosse T, et al. Molecular dissection of Salmonella-induced membrane ruffling versus invasion. Cell Microbiol. 2010;12:84-98 pubmed publisher
  7. Zhang S, Kamal M, Dahan K, Pawlak A, Ory V, Desvaux D, et al. c-mip impairs podocyte proximal signaling and induces heavy proteinuria. Sci Signal. 2010;3:ra39 pubmed publisher
    ..Together, these results identify c-mip as a key component in the molecular pathogenesis of acquired podocyte diseases. ..
  8. Park J, Kim Y, Lee S, Park J, Park Z, Sun W, et al. SNX18 shares a redundant role with SNX9 and modulates endocytic trafficking at the plasma membrane. J Cell Sci. 2010;123:1742-50 pubmed publisher
    ..Taken together, our results suggest that SNX18 functions with SNX9 in multiple pathways of endocytosis at the plasma membrane and that they are functionally redundant. ..
  9. Lee K, Gallop J, Rambani K, Kirschner M. Self-assembly of filopodia-like structures on supported lipid bilayers. Science. 2010;329:1341-5 pubmed publisher
    ..We propose that filopodia form via clustering of Arp2/3 complex activators, self-assembly of filopodial tip complexes on the membrane, and outgrowth of actin bundles...

More Information


  1. Zhang W, Wu Y, Wu C, Gunst S. Integrin-linked kinase regulates N-WASp-mediated actin polymerization and tension development in tracheal smooth muscle. J Biol Chem. 2007;282:34568-80 pubmed
    ..We conclude that the recruitment of the ILK.PINCH.alpha-parvin complex to membrane adhesion complexes is required to initiate cytoskeletal processes required for tension development in smooth muscle. ..
  2. Adamovich D, Nakamura F, Worth A, Burns S, Thrasher A, Hartwig J, et al. Activating mutations of N-WASP alter Shigella pathogenesis. Biochem Biophys Res Commun. 2009;384:284-9 pubmed publisher
    ..However, plaque numbers were increased in cells expressing N-WASP L229P and L232P. These data demonstrate that N-WASP structural conformation is an important regulator of Shigella pathogenesis in distinct segments of its lifecycle. ..
  3. Lyubimova A, Garber J, Upadhyay G, Sharov A, Anastasoaie F, Yajnik V, et al. Neural Wiskott-Aldrich syndrome protein modulates Wnt signaling and is required for hair follicle cycling in mice. J Clin Invest. 2010;120:446-56 pubmed publisher
    ..We therefore propose that N-WASP acts as a positive regulator of beta-catenin-dependent transcription, modulating differentiation of HF progenitor cells. ..
  4. Crepin V, Girard F, Schüller S, Phillips A, Mousnier A, Frankel G. Dissecting the role of the Tir:Nck and Tir:IRTKS/IRSp53 signalling pathways in vivo. Mol Microbiol. 2010;75:308-23 pubmed publisher
    ..Importantly, wild-type C. rodentium out-competed the tir tyrosine mutants during mixed infections. These results uncouple the Tir:Nck and Tir:IRTKS pathways from A/E lesion formation in vivo but assign them an important in vivo role...
  5. Ferrai C, Naum Onganía G, Longobardi E, Palazzolo M, Disanza A, Diaz V, et al. Induction of HoxB transcription by retinoic acid requires actin polymerization. Mol Biol Cell. 2009;20:3543-51 pubmed publisher
    ..We conclude that inducible Hox genes are selectively sensitive to the inhibition of actin polymerization and that actin polymerization is required for the assembly of a transcription complex on the regulatory region of the Hox genes. ..
  6. Tang D, Gunst S. The small GTPase Cdc42 regulates actin polymerization and tension development during contractile stimulation of smooth muscle. J Biol Chem. 2004;279:51722-8 pubmed
    ..Cdc42 may regulate the activation of neuronal Wiskott-Aldrich syndrome protein and the actin related protein complex, which in turn regulate actin filament polymerization initiated by the contractile stimulation of smooth muscle. ..
  7. Pichot C, Arvanitis C, Hartig S, Jensen S, Bechill J, Marzouk S, et al. Cdc42-interacting protein 4 promotes breast cancer cell invasion and formation of invadopodia through activation of N-WASp. Cancer Res. 2010;70:8347-56 pubmed publisher
    ..This study presents a new role for CIP4 in the promotion of migration and invasion of MDA-MB-231 breast cancer cells and establishes the contribution of F-BAR proteins to cancer cell motility and invasion. ..
  8. Lefever T, Pedersen E, Basse A, Paus R, Quondamatteo F, Stanley A, et al. N-WASP is a novel regulator of hair-follicle cycling that controls antiproliferative TGF{beta} pathways. J Cell Sci. 2010;123:128-40 pubmed publisher
    ..These data identify the actin nucleation regulator N-WASP as a novel element of hair-cycle control that modulates the antiproliferative and pro-apoptotic TGFbeta pathway in keratinocytes in vivo and in vitro. ..
  9. Bugyi B, Didry D, Carlier M. How tropomyosin regulates lamellipodial actin-based motility: a combined biochemical and reconstituted motility approach. EMBO J. 2010;29:14-26 pubmed publisher
    ..These results unveil the mechanism by which tropomyosin generates two morphologically and dynamically segregated actin networks from a single one. ..
  10. Duleh S, Welch M. WASH and the Arp2/3 complex regulate endosome shape and trafficking. Cytoskeleton (Hoboken). 2010;67:193-206 pubmed publisher
  11. Anitei M, Stange C, Parshina I, Baust T, Schenck A, Raposo G, et al. Protein complexes containing CYFIP/Sra/PIR121 coordinate Arf1 and Rac1 signalling during clathrin-AP-1-coated carrier biogenesis at the TGN. Nat Cell Biol. 2010;12:330-40 pubmed publisher
  12. Yamada H, Padilla Parra S, Park S, Itoh T, Chaineau M, Monaldi I, et al. Dynamic interaction of amphiphysin with N-WASP regulates actin assembly. J Biol Chem. 2009;284:34244-56 pubmed publisher
  13. Weflen A, Alto N, Hecht G. Tight junctions and enteropathogenic E. coli. Ann N Y Acad Sci. 2009;1165:169-74 pubmed publisher
    ..EspF, therefore, appears to hijack endocytic regulation via SNX9 and possibly N-WASP interaction, to affect an as yet unidentified pathogenic phenotype. ..
  14. Bu W, Lim K, Yu Y, Chou A, Sudhaharan T, Ahmed S. Cdc42 interaction with N-WASP and Toca-1 regulates membrane tubulation, vesicle formation and vesicle motility: implications for endocytosis. PLoS ONE. 2010;5:e12153 pubmed publisher
    ..Thus Cdc42 may influence endocytic membrane trafficking by regulating the formation and activity of the Toca-1/N-WASP complex. ..
  15. Lie P, Chan A, Mruk D, Lee W, Cheng C. Restricted Arp3 expression in the testis prevents blood-testis barrier disruption during junction restructuring at spermatogenesis. Proc Natl Acad Sci U S A. 2010;107:11411-6 pubmed publisher
  16. Hartig S, Ishikura S, Hicklen R, Feng Y, Blanchard E, Voelker K, et al. The F-BAR protein CIP4 promotes GLUT4 endocytosis through bidirectional interactions with N-WASp and Dynamin-2. J Cell Sci. 2009;122:2283-91 pubmed publisher
  17. Abella J, Vaillancourt R, Frigault M, Ponzo M, Zuo D, Sangwan V, et al. The Gab1 scaffold regulates RTK-dependent dorsal ruffle formation through the adaptor Nck. J Cell Sci. 2010;123:1306-19 pubmed publisher
    ..These data show that a Gab1-Nck signalling complex interacts with several RTKs to promote polarised actin remodelling and downstream biological responses...
  18. Chan G, Nogalski M, Yurochko A. Activation of EGFR on monocytes is required for human cytomegalovirus entry and mediates cellular motility. Proc Natl Acad Sci U S A. 2009;106:22369-74 pubmed publisher
    ..Together, these data provide evidence that EGFR plays an essential role in the immunopathobiology of HCMV by mediating viral entry into monocytes and stimulating the aberrant biological activity that promotes hematogenous dissemination. ..
  19. Staub E, Groene J, Heinze M, Mennerich D, Roepcke S, Klaman I, et al. An expression module of WIPF1-coexpressed genes identifies patients with favorable prognosis in three tumor types. J Mol Med (Berl). 2009;87:633-44 pubmed publisher
  20. Wang W, Zhong H, Xiao D, Huang X, Liao L, Xie Z, et al. The expression of CFL1 and N-WASP in esophageal squamous cell carcinoma and its correlation with clinicopathological features. Dis Esophagus. 2010;23:512-21 pubmed publisher
    ..These findings may have implications for targeted therapies in patients with ESCC. ..
  21. Misra A, Rajmohan R, Lim R, Bhattacharyya S, Thanabalu T. The mammalian verprolin, WIRE induces filopodia independent of N-WASP through IRSp53. Exp Cell Res. 2010;316:2810-24 pubmed publisher
    ..Thus our results show that Cdc42 regulates the activity of IRSp53 by regulating the IRSp53-WIRE interaction as well as localization of the complex to plasma membrane to generate filopodia. ..
  22. Takano K, Watanabe Takano H, Suetsugu S, Kurita S, Tsujita K, Kimura S, et al. Nebulin and N-WASP cooperate to cause IGF-1-induced sarcomeric actin filament formation. Science. 2010;330:1536-40 pubmed publisher
    ..These findings present the mechanisms of IGF-1-induced actin filament formation in myofibrillogenesis required for muscle maturation and hypertrophy and a mechanism of actin nucleation...
  23. Inoue T, Pattabiraman P, Epstein D, Vasantha Rao P. Effects of chemical inhibition of N-WASP, a critical regulator of actin polymerization on aqueous humor outflow through the conventional pathway. Exp Eye Res. 2010;90:360-7 pubmed publisher
  24. You J, Lin Chao S. Gas7 functions with N-WASP to regulate the neurite outgrowth of hippocampal neurons. J Biol Chem. 2010;285:11652-66 pubmed publisher
    ..The data demonstrate an essential role of Gas7 through its interaction with N-WASP during neuronal maturation/differentiation. ..
  25. Rivera G, Vasilescu D, Papayannopoulos V, Lim W, Mayer B. A reciprocal interdependence between Nck and PI(4,5)P(2) promotes localized N-WASp-mediated actin polymerization in living cells. Mol Cell. 2009;36:525-35 pubmed publisher
    ..This study uncovers a strong reciprocal interdependence between Nck and PI(4,5)P(2) in promoting localized N-WASp-mediated actin polymerization in cells. ..
  26. Fukumi Tominaga T, Mori Y, Matsuura A, Kaneko K, Matsui M, Ogata M, et al. DIP/WISH-deficient mice reveal Dia- and N-WASP-interacting protein as a regulator of cytoskeletal dynamics in embryonic fibroblasts. Genes Cells. 2009;14:1197-207 pubmed publisher
    ..Thus, DIP/WISH is a negative regulator of Rho and modulates cell adhesion by controlling the integration of adhesion molecules. ..
  27. Rottner K, Stradal T. Poxviruses taking a ride on actin: new users of known hardware. Cell Host Microbe. 2009;6:497-9 pubmed publisher
    ..Now, Dodding and Way identify functional orthologs of A36 in other vertebrate poxviruses that harbor varying numbers of Nck-binding sites and can substitute for A36 despite no sequence homology. ..
  28. Oser M, Yamaguchi H, Mader C, Bravo Cordero J, Arias M, Chen X, et al. Cortactin regulates cofilin and N-WASp activities to control the stages of invadopodium assembly and maturation. J Cell Biol. 2009;186:571-87 pubmed publisher
  29. Munderloh C, Solis G, Bodrikov V, Jaeger F, Wiechers M, Malaga Trillo E, et al. Reggies/flotillins regulate retinal axon regeneration in the zebrafish optic nerve and differentiation of hippocampal and N2a neurons. J Neurosci. 2009;29:6607-15 pubmed publisher
  30. Bomberger J, Maceachran D, Coutermarsh B, Ye S, O Toole G, Stanton B. Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles. PLoS Pathog. 2009;5:e1000382 pubmed publisher
  31. Weisswange I, Newsome T, Schleich S, Way M. The rate of N-WASP exchange limits the extent of ARP2/3-complex-dependent actin-based motility. Nature. 2009;458:87-91 pubmed publisher
    ..We suggest that the exchange rate of N-WASP controls the rate of ARP2/3-complex-dependent actin-based motility by regulating the extent of actin polymerization by antagonizing filament capping. ..
  32. Miller M, Lapetina S, MacGrath S, Sfakianos M, Pollard T, Koleske A. Regulation of actin polymerization and adhesion-dependent cell edge protrusion by the Abl-related gene (Arg) tyrosine kinase and N-WASp. Biochemistry. 2010;49:2227-34 pubmed publisher
    ..These results suggest that Arg promotes actin-based protrusions in response to extracellular stimuli through phosphorylation of and physical interactions with N-WASp. ..
  33. Derivery E, Gautreau A. Generation of branched actin networks: assembly and regulation of the N-WASP and WAVE molecular machines. Bioessays. 2010;32:119-31 pubmed publisher
    ..Moreover, this review attempts to put together diverse observations concerning the WAVE complex in the conceptual frame of an in vivo assembly pathway that has gained support from the recent identification of a precursor. ..
  34. Dodding M, Way M. Nck- and N-WASP-dependent actin-based motility is conserved in divergent vertebrate poxviruses. Cell Host Microbe. 2009;6:536-50 pubmed publisher
  35. Grassart A, Meas Yedid V, Dufour A, Olivo Marin J, Dautry Varsat A, Sauvonnet N. Pak1 phosphorylation enhances cortactin-N-WASP interaction in clathrin-caveolin-independent endocytosis. Traffic. 2010;11:1079-91 pubmed publisher
    ..Thus, Pak1, by controlling the interaction between cortactin and N-WASP, could regulate the polymerization of actin during clathrin-independent endocytosis. ..
  36. Dubielecka P, Cui P, Xiong X, Hossain S, Heck S, Angelov L, et al. Differential regulation of macropinocytosis by Abi1/Hssh3bp1 isoforms. PLoS ONE. 2010;5:e10430 pubmed publisher
    ..These findings also raise the possibility that isoform-specific roles occur in other Abi1 functions. ..
  37. Rhoads D, Guan J. Analysis of directional cell migration on defined FN gradients: role of intracellular signaling molecules. Exp Cell Res. 2007;313:3859-67 pubmed