Summary: A type VI intermediate filament protein expressed mostly in nerve cells where it is associated with the survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Top Publications

  1. Zimmerman L, Parr B, Lendahl U, Cunningham M, McKay R, Gavin B, et al. Independent regulatory elements in the nestin gene direct transgene expression to neural stem cells or muscle precursors. Neuron. 1994;12:11-24 pubmed
    ..Neuroepithelial stem cells express the intermediate filament protein nestin and down-regulate it sharply at the transition from proliferating stem cell to postmitotic neuron...
  2. Higashida H, Liang M, Yoshihara T, Akther S, Fakhrul A, Stanislav C, et al. An immunohistochemical, enzymatic, and behavioral study of CD157/BST-1 as a neuroregulator. BMC Neurosci. 2017;18:35 pubmed publisher
    ..CD157 immunoreactivity colocalized with nestin-positive cells and elements in the ventricular zones in E17 embryos...
  3. Chen J, Mao S, Li H, Zheng M, Yi L, Lin J, et al. The pathological structure of the perivascular niche in different microvascular patterns of glioblastoma. PLoS ONE. 2017;12:e0182183 pubmed publisher
    ..of different microvascular pattern niches (MVPNs) in GBM by detecting the expression of CD34, CD133, Nestin, ?-SMA, GFAP and CD14 in the perivascular niche using multiple -fluorescence...
  4. Du Z, Cai C, Sims M, Boop F, Davidoff A, Pfeffer L. The effects of type I interferon on glioblastoma cancer stem cells. Biochem Biophys Res Commun. 2017;491:343-348 pubmed publisher
    ..In addition, we show that IFN induces transient STAT3 activation in GSCs, while induction of astrocytic differentiation in GSCs results in sustained STAT3 activation. ..
  5. Renault Mihara F, Mukaino M, Shinozaki M, Kumamaru H, Kawase S, Baudoux M, et al. Regulation of RhoA by STAT3 coordinates glial scar formation. J Cell Biol. 2017;216:2533-2550 pubmed publisher
    ..By specific targeting of lesion-proximal, reactive astrocytes in Nestin-Cre mice, we show that reduction of PTEN rescues glial scar formation in Nestin-Stat3+/-
  6. Renfranz P, Cunningham M, McKay R. Region-specific differentiation of the hippocampal stem cell line HiB5 upon implantation into the developing mammalian brain. Cell. 1991;66:713-29 pubmed
    ..precursors to the distinct cell types constituting the mammalian brain can be identified by the presence of the nestin intermediate filament...
  7. Tardif K, Hertig V, Dumais C, Villeneuve L, PERRAULT L, Tanguay J, et al. Nestin downregulation in rat vascular smooth muscle cells represents an early marker of vascular disease in experimental type I diabetes. Cardiovasc Diabetol. 2014;13:119 pubmed publisher
    b>Nestin was reported to directly contribute to cell proliferation and the intermediate filament protein was detected in vascular smooth muscle cells...
  8. Rusu M, Poalelungi C, Vrapciu A, Păduraru L, Didilescu A, Stan C. Anoctamin 1 positive esophageal interstitial Cajal cells in late stage human embryos. Anat Rec (Hoboken). 2014;297:301-7 pubmed
    ..performed for a series of antibodies: a-smooth muscle actin (a-SMA), desmin, CD31, CD34, CD117/c-kit, DOG1, and nestin. Longitudinal and circular muscle layers were a-SMA1/desmin1/nestin1...
  9. Stuelsatz P, Keire P, Yablonka Reuveni Z. Isolation, Culture, and Immunostaining of Skeletal Muscle Myofibers from Wildtype and Nestin-GFP Mice as a Means to Analyze Satellite Cell. Methods Mol Biol. 2017;1556:51-102 pubmed publisher
    ..We conclude this chapter by promoting the Nestin-GFP transgenic mouse as an efficient tool for direct analysis of satellite cells in isolated myofibers...

More Information


  1. Krishnasamy S, Weng Y, Thammisetty S, Phaneuf D, Lalancette Hébert M, Kriz J. Molecular imaging of nestin in neuroinflammatory conditions reveals marked signal induction in activated microglia. J Neuroinflammation. 2017;14:45 pubmed publisher
    b>Nestin is a known marker of neuronal progenitor cells in the adult brain. Following neuro- and gliogenesis, nestin is replaced by cell type-specific intermediate filaments, e.g...
  2. Jaworski D, Kelly G, Hockfield S. Intracranial injury acutely induces the expression of the secreted isoform of the CNS-specific hyaluronan-binding protein BEHAB/brevican. Exp Neurol. 1999;157:327-37 pubmed
    ..of BEHAB/brevican expression parallel the gliotic response and the expression of the intermediate filament protein nestin. The up-regulation of BEHAB/brevican in response to CNS injury suggests a role for this extracellular matrix ..
  3. Coste C, Neirinckx V, Sharma A, Agirman G, Rogister B, Foguenne J, et al. Human bone marrow harbors cells with neural crest-associated characteristics like human adipose and dermis tissues. PLoS ONE. 2017;12:e0177962 pubmed publisher
    ..It appeared that human bone marrow, similarly to adipose tissue and dermis, contains NESTIN+ / SOX9+ / TWIST+ / SLUG+ / P75NTR+ / BRN3A+/ MSI1+/ SNAIL1+ cells and were able to differentiate into melanocytes,..
  4. Leduc C, Etienne Manneville S. Regulation of microtubule-associated motors drives intermediate filament network polarization. J Cell Biol. 2017;216:1689-1703 pubmed publisher
    ..and photoconversion experiments in glial cells expressing vimentin, glial fibrillary acidic protein, and nestin, we show that the distribution of cytoplasmic IFs results from a continuous turnover based on the cooperation of ..
  5. Alexandrova E, Talos F, Moll U. p73 is dispensable for commitment to neural stem cell fate, but is essential for neural stem cell maintenance and for blocking premature differentiation. Cell Death Differ. 2013;20:368 pubmed publisher
  6. Levison S, Goldman J. Multipotential and lineage restricted precursors coexist in the mammalian perinatal subventricular zone. J Neurosci Res. 1997;48:83-94 pubmed
    ..Therefore, we conclude that the perinatal SVZ is a mosaic of multipotential, bipotential, and lineage restricted precursors, and that the lack of postnatal neocortical neurogenesis is not due to the absence of potential neuroblasts. ..
  7. Amoh Y, Kanoh M, Niiyama S, Kawahara K, Sato Y, Katsuoka K, et al. Human and mouse hair follicles contain both multipotent and monopotent stem cells. Cell Cycle. 2009;8:176-7 pubmed
  8. Birbrair A, Zhang T, Files D, Mannava S, Smith T, Wang Z, et al. Type-1 pericytes accumulate after tissue injury and produce collagen in an organ-dependent manner. Stem Cell Res Ther. 2014;5:122 pubmed publisher
    ..Here, we report the presence of two pericyte subtypes, type-1 (Nestin-GFP-/NG2-DsRed+) and type-2 (Nestin-GFP+/NG2-DsRed+), surrounding blood vessels in lungs, kidneys, heart, spinal ..
  9. Brulet R, Zhu J, Aktar M, Hsieh J, Cho K. Mice with conditional NeuroD1 knockout display reduced aberrant hippocampal neurogenesis but no change in epileptic seizures. Exp Neurol. 2017;293:190-198 pubmed publisher
    ..As expected, conditional knockout (cKO) of NeuroD1 in Nestin-expressing stem/progenitors and their progeny led to a reduction in the number of NeuroD1+ adult-generated neurons ..
  10. Jossin Y, Lee M, Klezovitch O, Kon E, Cossard A, Lien W, et al. Llgl1 Connects Cell Polarity with Cell-Cell Adhesion in Embryonic Neural Stem Cells. Dev Cell. 2017;41:481-495.e5 pubmed publisher
    ..We report here that mice with embryonic neural stem-cell-specific deletion of Llgl1 (Nestin-Cre/Llgl1fl/fl), a mammalian ortholog of the Drosophila cell polarity gene lgl, exhibit MCCs resembling ..
  11. Jin Y, Barnett A, Zhang Y, Yu X, Luo Y. Poststroke Sonic Hedgehog Agonist Treatment Improves Functional Recovery by Enhancing Neurogenesis and Angiogenesis. Stroke. 2017;48:1636-1645 pubmed publisher
    ..We used a genetically inducible neural stem cell-specific reporter mouse line (nestin-CreERT2-R26R-YFP) to label and track their proliferation, survival, and differentiation in ischemic brain...
  12. Wojcinski A, Lawton A, Bayin N, Lao Z, Stephen D, Joyner A. Cerebellar granule cell replenishment postinjury by adaptive reprogramming of Nestin+ progenitors. Nat Neurosci. 2017;20:1361-1370 pubmed publisher
    ..We demonstrate that following postnatal ablation of granule cell progenitors, Nestin-expressing progenitors, specified during mid-embryogenesis to produce astroglia and interneurons, switch their ..
  13. Balani D, Ono N, Kronenberg H. Parathyroid hormone regulates fates of murine osteoblast precursors in vivo. J Clin Invest. 2017;127:3327-3338 pubmed publisher
    ..Thus, teriparatide increases the numbers of early cells of the osteoblast lineage, hastens their differentiation into osteoblasts, and suppresses their differentiation into adipocytes in vivo. ..
  14. Hertig V, Tardif K, Meus M, Duquette N, Villeneuve L, Toussaint F, et al. Nestin expression is upregulated in the fibrotic rat heart and is localized in collagen-expressing mesenchymal cells and interstitial CD31(+)- cells. PLoS ONE. 2017;12:e0176147 pubmed publisher the accumulation of collagen-immunoreactive mesenchymal cells expressing the intermediate filament protein nestin. The present study tested the hypothesis that nestin expression was increased in the hypertrophied/fibrotic left ..
  15. Hu P, Xia Q, Wu F, Li D, Qi Y, Hu Y, et al. NSPc1 promotes cancer stem cell self-renewal by repressing the synthesis of all-trans retinoic acid via targeting RDH16 in malignant glioma. Oncogene. 2017;36:4706-4718 pubmed publisher
    ..impaired neurosphere formation and self-renewal abilities, down-regulated expression of stemness markers such as NESTIN, CD133 and SOX2, and decreased capacity to propagate subcutaneous xenografts...
  16. Tropepe V, Coles B, Chiasson B, Horsford D, Elia A, McInnes R, et al. Retinal stem cells in the adult mammalian eye. Science. 2000;287:2032-6 pubmed
  17. Yuan T, Liao W, Feng N, Lou Y, Niu X, Zhang A, et al. Human induced pluripotent stem cell-derived neural stem cells survive, migrate, differentiate, and improve neurologic function in a rat model of middle cerebral artery occlusion. Stem Cell Res Ther. 2013;4:73 pubmed publisher
    ..Implanted iPSC-derived NSCs were able to survive, migrate into the ischemic brain area to differentiate into mature neural cells, and seem to have potential to restore lost neurologic function from damage due to stroke in a rat model. ..
  18. Tatebayashi Y, Iqbal K, Grundke Iqbal I. Dynamic regulation of expression and phosphorylation of tau by fibroblast growth factor-2 in neural progenitor cells from adult rat hippocampus. J Neurosci. 1999;19:5245-54 pubmed
    ..These results demonstrate that proliferating adult rat hippocampal progenitor cells express adult isoforms of tau stably and that FGF-2 upregulates the expression and, by upregulating GSK-3 activity, the phosphorylation of tau. ..
  19. Pan M, Weng Y, Sun Y. Overexpression of Dentin matrix protein 1 in Nestin+ cells causes bone loss in mouse long bone. Biochem Biophys Res Commun. 2017;490:356-363 pubmed publisher
    ..Recently, it has been found that DMP1 is also expressed in other cell types, such as chondrocytes. Nestin+ cells are one important type of progenitor cell in bone marrow and are associated with bone remodeling...
  20. Gordon R, Zhang L, Yang Z. Restore the brake on tumor progression. Biochem Pharmacol. 2017;138:1-6 pubmed publisher
    ..We recently revealed that during MB formation, tumor cells express Nestin, a type VI intermediate filament protein, which maintains uncontrolled Shh signaling by abolishing negative ..
  21. Mii S, Uehara F, Yano S, Tran B, Miwa S, Hiroshima Y, et al. Nestin-Expressing Stem Cells Promote Nerve Growth in Long-Term 3-Dimensional Gelfoam®-Supported Histoculture. PLoS ONE. 2013;8:e67153 pubmed publisher
    We have previously reported that hair follicles contain multipotent stem cells which express nestin. The nestin-expressing cells form the hair follicle sensory nerve...