nitrilotriacetic acid

Summary

Summary: A derivative of acetic acid, N(CH2COOH)3. It is a complexing (sequestering) agent that forms stable complexes with Zn2+. (From Miall's Dictionary of Chemistry, 5th ed.)

Top Publications

  1. Li Y, Lin Y, Tsai P, Chen C, Chen W, Chen Y. Nitrilotriacetic acid-coated magnetic nanoparticles as affinity probes for enrichment of histidine-tagged proteins and phosphorylated peptides. Anal Chem. 2007;79:7519-25 pubmed
    We herein demonstrate superparamagnetic Fe3O4 nanoparticles coated with nitrilotriacetic acid derivative (NTA) that can bind with different immobilized metal ions are capable of probing diverse target species...
  2. Baur J, Gondran C, Holzinger M, Defrancq E, Perrot H, Cosnier S. Label-free femtomolar detection of target DNA by impedimetric DNA sensor based on poly(pyrrole-nitrilotriacetic acid) film. Anal Chem. 2010;82:1066-72 pubmed publisher
    ..A linear quantification of the HIV DNA target was carried out in the range 10(-15) to 10(-8) mol L(-1)...
  3. Roullier V, Clarke S, You C, Pinaud F, Gouzer G, Schaible D, et al. High-affinity labeling and tracking of individual histidine-tagged proteins in live cells using Ni2+ tris-nitrilotriacetic acid quantum dot conjugates. Nano Lett. 2009;9:1228-34 pubmed publisher
    ..To address these challenges, we designed, characterized and applied QD-trisNTA, which integrates tris-nitrilotriacetic acid, a small and high-affinity recognition unit for the ubiquitous polyhistidine protein tag...
  4. Kim M, Park H, Kim J, Ryu J, Hong S, Han S, et al. Western blot analysis using metal-nitrilotriacetate conjugated CdSe/ZnS quantum dots. Anal Biochem. 2008;379:124-6 pubmed publisher
    ..Interestingly, under our conditions, cobalt-NTA showed better detection ability than did nickel-NTA. Our new method may be able to facilitate and simplify the routinely used protein detection procedure...
  5. Bhagawati M, Lata S, Tampe R, Piehler J. Native laser lithography of His-tagged proteins by uncaging of multivalent chelators. J Am Chem Soc. 2010;132:5932-3 pubmed publisher
    ..Thus, versatile, user-defined protein micropatterns can be assembled under physiological conditions with a standard confocal laser-scanning microscope...
  6. Chevalier S, Cuestas Ayllon C, Grazu V, Luna M, Feracci H, de la Fuente J. Creating biomimetic surfaces through covalent and oriented binding of proteins. Langmuir. 2010;26:14707-15 pubmed publisher
    ..The main innovation of this methodology consists of the covalent binding between the nitrilotriacetic acid (NTA) moiety and the proteins, ensuring not only orientation, but also stability of the recombinant ..
  7. Lata S, Gavutis M, Tampe R, Piehler J. Specific and stable fluorescence labeling of histidine-tagged proteins for dissecting multi-protein complex formation. J Am Chem Soc. 2006;128:2365-72 pubmed
    ..A broad application of these fluorescence conjugates for protein interaction analysis can be envisaged...
  8. Boonyarattanakalin S, Athavankar S, Sun Q, Peterson B. Synthesis of an artificial cell surface receptor that enables oligohistidine affinity tags to function as metal-dependent cell-penetrating peptides. J Am Chem Soc. 2006;128:386-7 pubmed
    ..surface receptor comprising an N-alkyl derivative of 3beta-cholesterylamine linked to the metal chelator nitrilotriacetic acid (NTA)...
  9. Sultana S, Prasad L, Jahangir T. Luteolin ameliorates ferric nitrilotriacetic acid induced renal toxicity and tumor promotional response in rat. Indian J Exp Biol. 2009;47:355-60 pubmed
    Ferric nitrilotriacetic acid (Fe-NTA) (9 mg Fe/kg body weight, i.p.) caused significant depletion in the detoxification and antioxidant enzyme armory with concomitant elevation in renal lipidperoxidation, serum toxicity markers viz...

More Information

Publications91

  1. Xing B, Yin X. Electrochemiluminescence from hydrophilic thin film Ru(bpy)3(2+)-modified electrode prepared using natural halloysite nanotubes and polyacrylamide gel. Biosens Bioelectron. 2009;24:2939-42 pubmed publisher
    ..The different electrochemiluminescence (ECL) behaviors of the electrode using nitrilotriacetic acid and tripropylamine, as co-reactants, illustrate the hydrophilic character of the modified electrode...
  2. Watson D, Platt V, Cao L, Venditto V, Szoka F. Antibody response to polyhistidine-tagged peptide and protein antigens attached to liposomes via lipid-linked nitrilotriacetic acid in mice. Clin Vaccine Immunol. 2011;18:289-97 pubmed publisher
    ..In this report, we evaluate noncovalent metal chelation via nitrilotriacetic acid (NTA) as a nondestructive method to attach peptide and protein antigens to liposomes...
  3. Valiokas R, Klenkar G, Tinazli A, Tampe R, Liedberg B, Piehler J. Differential protein assembly on micropatterned surfaces with tailored molecular and surface multivalency. Chembiochem. 2006;7:1325-9 pubmed
  4. Goldsmith C, Jaworski J, Sheng M, Lippard S. Selective labeling of extracellular proteins containing polyhistidine sequences by a fluorescein-nitrilotriacetic acid conjugate. J Am Chem Soc. 2006;128:418-9 pubmed
    The compound NTA-DCF consists of two components, a dichlorofluorescein (DCF) reporter and a nitrilotriacetic acid (NTA) functionality. The latter binds polyhistidine sequences selectively through a bridging metal ion...
  5. Johnson D, Martin L. Controlling protein orientation at interfaces using histidine tags: an alternative to Ni/NTA. J Am Chem Soc. 2005;127:2018-9 pubmed
  6. Xu C, Xu K, Gu H, Zheng R, Liu H, Zhang X, et al. Dopamine as a robust anchor to immobilize functional molecules on the iron oxide shell of magnetic nanoparticles. J Am Chem Soc. 2004;126:9938-9 pubmed
    ..Using nitrilotriacetic acid (NTA) as the functional molecule, we created a system with an M/Fe2O3-DA-NTA (M = Co or SmCo5...
  7. Guignet E, Hovius R, Vogel H. Reversible site-selective labeling of membrane proteins in live cells. Nat Biotechnol. 2004;22:440-4 pubmed
    ..We investigated the ionotropic 5HT(3) serotonin receptor by fluorescence measurements to characterize in vivo the probe-receptor interactions, yielding information on structure and plasma membrane distribution of the receptor...
  8. Jiang D, Li X, Williams R, Patel S, Men L, Wang Y, et al. Ternary complexes of iron, amyloid-beta, and nitrilotriacetic acid: binding affinities, redox properties, and relevance to iron-induced oxidative stress in Alzheimer's disease. Biochemistry. 2009;48:7939-47 pubmed publisher
    ..Abeta(1-16), and the Fe(III) or Fe(II) complex with nitrilotriacetic acid (NTA)...
  9. Grunwald C, Schulze K, Reichel A, Weiss V, Blaas D, Piehler J, et al. In situ assembly of macromolecular complexes triggered by light. Proc Natl Acad Sci U S A. 2010;107:6146-51 pubmed publisher
    ..This light-triggered molecular recognition should allow for a spatiotemporal control of protein-protein interactions and cellular processes by light-triggered protein clustering...
  10. Kim S, Jeyakumar M, Katzenellenbogen J. Dual-mode fluorophore-doped nickel nitrilotriacetic acid-modified silica nanoparticles combine histidine-tagged protein purification with site-specific fluorophore labeling. J Am Chem Soc. 2007;129:13254-64 pubmed
    ..23 nm particle, were surface modified with nitrilotriacetic acid (NTA), producing TMR-SiO2-NTA-Ni2+...
  11. Tang Y, Chakraborty R, Martin H, Chu J, Hazen T, Keasling J. Flux analysis of central metabolic pathways in Geobacter metallireducens during reduction of soluble Fe(III)-nitrilotriacetic acid. Appl Environ Microbiol. 2007;73:3859-64 pubmed
    ..Acetate labeled in the first or second position was the sole carbon source, and Fe-nitrilotriacetic acid was the sole terminal electron acceptor...
  12. Liao Y, Cheng Y, Li Q. Preparation of nitrilotriacetic acid/Co2+-linked, silica/boron-coated magnetite nanoparticles for purification of 6 x histidine-tagged proteins. J Chromatogr A. 2007;1143:65-71 pubmed
    In this report, we describe the preparation of novel nitrilotriacetic acid/Co2+-linked, silica/boron-coated magnetite nanoparticles for purification of 6 x His-tagged proteins...
  13. Lata S, Reichel A, Brock R, Tampe R, Piehler J. High-affinity adaptors for switchable recognition of histidine-tagged proteins. J Am Chem Soc. 2005;127:10205-15 pubmed
    ..Several supramolecular entities containing 2-4 nitrilotriacetic acid (NTA) moieties were synthesized, which additionally contained an amino group, to which fluorescein was ..
  14. Xu C, Xu K, Gu H, Zhong X, Guo Z, Zheng R, et al. Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins. J Am Chem Soc. 2004;126:3392-3 pubmed
    ..5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins...
  15. Khan F, He M, Taussig M. Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfaces. Anal Chem. 2006;78:3072-9 pubmed
    ..The double-His6 peptide has the potential to be a universal tag for protein immobilization and detection on arrays and single-step purification of proteins from crude mixtures...
  16. Kang E, Park J, McClellan S, Kim J, Holland D, Lee G, et al. Specific adsorption of histidine-tagged proteins on silica surfaces modified with Ni2+/NTA-derivatized poly(ethylene glycol). Langmuir. 2007;23:6281-8 pubmed
    Silica surfaces modified with nitrilotriacetic acid (NTA)-polyethylene glycol (PEG) derivatives were used to immobilize hexahistidine-tagged green fluorescent protein (His6-GFP), biotin/streptavidin-AlexaFluor555 (His6-biotin/SA-AF), and ..
  17. Schmidt J, Guillaume P, Irving M, Baumgaertner P, Speiser D, Luescher I. Reversible major histocompatibility complex I-peptide multimers containing Ni(2+)-nitrilotriacetic acid peptides and histidine tags improve analysis and sorting of CD8(+) T cells. J Biol Chem. 2011;286:41723-35 pubmed publisher
    ..They are built on reversible chelate complexes of Ni(2+)-nitrilotriacetic acid (NTA) with oligohistidines...
  18. Peneva K, Mihov G, Herrmann A, Zarrabi N, Börsch M, Duncan T, et al. Exploiting the nitrilotriacetic acid moiety for biolabeling with ultrastable perylene dyes. J Am Chem Soc. 2008;130:5398-9 pubmed publisher
    ..The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence ..
  19. Crowe J, Dobeli H, Gentz R, Hochuli E, Stuber D, Henco K. 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification. Methods Mol Biol. 1994;31:371-87 pubmed
  20. Min K, Nishida K, Onosaka S. Protective effect of metallothionein to ras DNA damage induced by hydrogen peroxide and ferric ion-nitrilotriacetic acid. Chem Biol Interact. 1999;122:137-52 pubmed
    ..induced by hydroxyl radical, the effects of MTs on DNA strand scission due to incubation with ferric ion-nitrilotriacetic acid and H2O2 (Fe3+ -NTA/H2O2) were studied...
  21. Kapanidis A, Ebright Y, Ebright R. Site-specific incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni(2+):nitrilotriacetic Acid (n)-fluorochrome conjugates. J Am Chem Soc. 2001;123:12123-5 pubmed
  22. Altin J, White F, Easton C. Synthesis of the chelator lipid nitrilotriacetic acid ditetradecylamine (NTA-DTDA) and its use with the IAsys biosensor to study receptor-ligand interactions on model membranes. Biochim Biophys Acta. 2001;1513:131-48 pubmed
    This work describes the synthesis and use of the chelator lipid, nitrilotriacetic acid ditetradecylamine (NTA-DTDA)...
  23. Meena B, Anburajan L, Vinithkumar N, Shridhar D, Raghavan R, Dharani G, et al. Molecular expression of l-asparaginase gene from Nocardiopsis alba NIOT-VKMA08 in Escherichia coli: A prospective recombinant enzyme for leukaemia chemotherapy. Gene. 2016;590:220-6 pubmed publisher
    ..The recombinant enzyme was purified using nickel-nitrilotriacetic acid (Ni-NTA) chromatography and the purified enzyme disclosed an elevated level of asparaginase activity (158...
  24. Li N, Yi L, He Z, Zhang W, Li H, Lin J. A DNA-directed covalent conjugation fluorescence probe for in vitro detection of functional matrix metalloproteinases. Analyst. 2017;142:634-640 pubmed publisher
    ..to design a fluorescence probe for in vitro detection of functional matrix metalloproteinases, by which a nitrilotriacetic acid (NTA)-modified DNA probe can combine with the Zn2+ in the active site of MMPs, and then a ..
  25. Crosbie G, Lodi A, McB Miller J, Skellern G. The control of nitrilotriacetic acid in edetic acid and its salts by liquid chromatography. J Pharm Biomed Anal. 2003;33:435-40 pubmed
    Two liquid chromatographic methods have been developed and validated for the determination of nitrilotriacetic acid (NTA) in edetic acid (EDTA) and its salts...
  26. Tanaka Y, Aleksunes L, Goedken M, Chen C, Reisman S, Manautou J, et al. Coordinated induction of Nrf2 target genes protects against iron nitrilotriacetate (FeNTA)-induced nephrotoxicity. Toxicol Appl Pharmacol. 2008;231:364-73 pubmed publisher
    ..Collectively, these results suggest that activation of Nrf2 protects mouse kidneys from FeNTA-induced oxidative stress damage by coordinately up-regulating the expression of cytoprotective genes. ..
  27. Toyokuni S, Itani T, Morimitsu Y, Okada K, Ozeki M, Kondo S, et al. Protective effect of colored rice over white rice on Fenton reaction-based renal lipid peroxidation in rats. Free Radic Res. 2002;36:583-92 pubmed
    ..These may partly explain the antioxidative effect. Furthermore, colored strains of rice are rich in proteins. Thus, our data warrants further investigation of the antioxidative effect of colored rice. ..
  28. Deo R, Rittmann B, Reed D. Bacterial Pu(V) reduction in the absence and presence of Fe(III)-NTA: modeling and experimental approach. Biodegradation. 2011;22:921-9 pubmed publisher
    ..Besides Fe, contaminated sites often contain strong complexing ligands, such as nitrilotriacetic acid (NTA)...
  29. Ansar S, Iqbal M. Antioxidant and nephroprotective potential of butylated hydroxyanisole against ferric nitrilotriacetate-induced oxidative stress and early tumor events. Hum Exp Toxicol. 2016;35:448-53 pubmed publisher
    ..Pretreatment with BHA prior to Fe-NTA treatment increased GSH and the activities of antioxidant enzymes to a range of 1.5- to 2-fold in kidney. The results indicate that BHA suppresses Fe-NTA-induced nephrotoxicity in male Wistar rats. ..
  30. Lenoir G, Menguy T, Corre F, Montigny C, Pedersen P, Thinès D, et al. Overproduction in yeast and rapid and efficient purification of the rabbit SERCA1a Ca(2+)-ATPase. Biochim Biophys Acta. 2002;1560:67-83 pubmed
    ..5 to 7.3 micromol ATP hydrolyzed/min/mg Ca(2+)-ATPase. It was stable and active for several days at 4 degrees C or after removal of DM with Bio-beads and storage at -80 degrees C. ..
  31. Liu S, Bischoff K, Anderson A, Rich J. Novel Feruloyl Esterase from Lactobacillus fermentum NRRL B-1932 and Analysis of the Recombinant Enzyme Produced in Escherichia coli. Appl Environ Microbiol. 2016;82:5068-76 pubmed publisher
    ..The recombinant LfFE containing a polyhistidine tag was purified by nickel-nitrilotriacetic acid affinity resin...
  32. Lata S, Piehler J. Stable and functional immobilization of histidine-tagged proteins via multivalent chelator headgroups on a molecular poly(ethylene glycol) brush. Anal Chem. 2005;77:1096-105 pubmed
    ..Multivalent chelator heads (MCH) carrying two and three nitrilotriacetic acid (NTA) moieties were coupled with controlled surface concentration to glass surfaces premodified with an ..
  33. Jia H, Chen H, Nulaji G, Li X, Wang C. Effect of low-molecular-weight organic acids on photo-degradation of phenanthrene catalyzed by Fe(III)-smectite under visible light. Chemosphere. 2015;138:266-71 pubmed publisher
    ..e., malic acid, oxalic acid, citric acid, ethylenediaminetetraacetic acid (EDTA), and nitrilotriacetic acid. Our results show that oxalic acid is most effective in promoting the photodegradation of phenanthrene, ..
  34. Yoshitani N, Saito K, Saikawa W, Asanuma M, Yokoyama S, Hirota H. NTA-mediated protein capturing strategy in screening experiments for small organic molecules by surface plasmon resonance. Proteomics. 2007;7:494-499 pubmed publisher
  35. Hseu Z, Jien S, Wang S, Deng H. Using EDDS and NTA for enhanced phytoextraction of Cd by water spinach. J Environ Manage. 2013;117:58-64 pubmed publisher
    ..experiment was used to test the applicability of [S,S]-Ethylenediaminedisuccinic acid (EDDS) and nitrilotriacetic acid (NTA) at rates of 2.5 mmol kg(-1) and 5...
  36. Wang N, Wang Z, Niu X, Yang X. Synthesis, characterization and anti-diabetic therapeutic potential of novel aminophenol-derivatized nitrilotriacetic acid vanadyl complexes. J Inorg Biochem. 2015;152:104-13 pubmed publisher
    In the present work, we synthesized three novel aminophenol-derivatized nitrilotriacetic acid vanadyl complexes (VOohpada, VOmhpada, VOphpada) using the strategy of rational incorporation of antioxidant groups in ligand in order to ..
  37. Jang J, Chang I, Moon H, Kang K, Kim B. Nitrilotriacetic acid degradation under microbial fuel cell environment. Biotechnol Bioeng. 2006;95:772-4 pubmed
    The removal of nitrilotriacetic acid (NTA) was studied under anaerobic conditions using oligotrophic and copiotrophic microbial fuel cells (MFCs) as a novel wastewater treatment process...
  38. Kohli B, Ostermeier C. A Rubredoxin based system for screening of protein expression conditions and on-line monitoring of the purification process. Protein Expr Purif. 2003;28:362-7 pubmed
    ..Further, it allows direct on-line readout on conventional chromatography systems, holding promise for automated multi-step chromatography. ..
  39. Balland V, Lecomte S, Limoges B. Characterization of the electron transfer of a ferrocene redox probe and a histidine-tagged hemoprotein specifically bound to a nitrilotriacetic-terminated self-assembled monolayer. Langmuir. 2009;25:6532-42 pubmed publisher
    ..The influence of protein immobilization on E0' is discussed together with the long-range ET rate constant and molecular orientation of the surface-immobilized hNb. ..
  40. Guo H, Ekusa A, Iwai K, Yonekura M, Takahata Y, Morimatsu F. Royal jelly peptides inhibit lipid peroxidation in vitro and in vivo. J Nutr Sci Vitaminol (Tokyo). 2008;54:191-5 pubmed
    ..01) in Group Fe than in Group C. TC (p<0.05) and LPO levels (p<0.01) were lower in Group Fe+R than in Group Fe. Our data suggest that RJPx may inhibit LPO both in vitro and in vivo. ..
  41. Yalaz M, Levent E, Olukman M, Calkavur S, Akisu M, Kultursay N. Role of digoxin-like immunoreactive substance in the pathogenesis of transient tachypnea of newborn. Biomed Res Int. 2013;2013:704763 pubmed publisher
  42. Friedrich M, Kirste V, Zhu J, Gennis R, Knoll W, Naumann R. Activity of membrane proteins immobilized on surfaces as a function of packing density. J Phys Chem B. 2008;112:3193-201 pubmed publisher
    ..Electrostatic and other interaction forces between protein molecules are held responsible for these effects. ..
  43. Meers E, Hopgood M, Lesage E, Vervaeke P, Tack F, Verloo M. Enhanced phytoextraction: in search of EDTA alternatives. Int J Phytoremediation. 2004;6:95-109 pubmed
  44. Wang W, Wang D, Li Z. Facile fabrication of recyclable and active nanobiocatalyst: purification and immobilization of enzyme in one pot with Ni-NTA functionalized magnetic nanoparticle. Chem Commun (Camb). 2011;47:8115-7 pubmed publisher
  45. Atif F, Kaur M, Yousuf S, Raisuddin S. In vitro free radical scavenging activity of hepatic metallothionein induced in an Indian freshwater fish, Channa punctata Bloch. Chem Biol Interact. 2006;162:172-80 pubmed
    ..Findings of the present study strongly suggest a free radical scavenging role for fish MT. Present study adds to the little existing knowledge about fish MT and its possible biological functions. ..
  46. Vidigal S, Tóth I, Rangel A. Determination of total protein content in white wines by solid phase spectrometry in a SI-LOV system. Talanta. 2012;96:102-6 pubmed publisher
    ..The method is based on the retention of the proteins in the solid support, NTA (nitrilotriacetic acid) superflow beads, charged by Cu(2+)...
  47. Wang W, Dong J, Baker G, Bruening M. Bifunctional polymer brushes for low-bias enrichment of mono- and multi-phosphorylated peptides prior to mass spectrometry analysis. Analyst. 2011;136:3595-8 pubmed publisher
    ..Polymer brushes orthogonally derivatized with oxotitanium and nitrilotriacetate-Fe(III) groups enrich both mono- and multi-phosphorylated peptides for mass spectrometry analysis. ..
  48. He Q, Woodworth R, Chasteen N. Anion exchange in human serum transferrin N-lobe: a model study with variant His249Ala. J Biol Inorg Chem. 2003;8:635-43 pubmed
    ..The overall apparent equilibrium constant for formation of Fe-H249A-NTA from Fe-H249A-CO(3) is K'=442 at pH 7.4. The NTA complex is favored over the carbonate complex both kinetically and thermodynamically in the pH range 7.4-8.2. ..
  49. Monahan M, Teel A, Watts R. Displacement of five metals sorbed on kaolinite during treatment with modified Fenton's reagent. Water Res. 2005;39:2955-63 pubmed
  50. Wang M, Jiao Y, Cheng C, Hua J, Yang Y. Nitrogen-doped carbon quantum dots as a fluorescence probe combined with magnetic solid-phase extraction purification for analysis of folic acid in human serum. Anal Bioanal Chem. 2017;409:7063-7075 pubmed publisher
    ..nitrogen-doped carbon quantum dots (N-CQDs), simply prepared using a one-step hydrothermal method with nitrilotriacetic acid, could be selectively quenched by FA...
  51. Kimura Y, Ono T. Functional and structural study on chelator-induced suppression of S2/S1 FTIR spectrum in photosynthetic oxygen-evolving complex. J Inorg Biochem. 2003;97:231-9 pubmed
    ..Chelators with carboxylate and/or carboxymethyl groups bound to a single nitrogen [nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA)] or carbon (citric acid) were relatively ineffective for the ..
  52. Deiana M, Rosa A, Corona G, Atzeri A, Incani A, Visioli F, et al. Protective effect of olive oil minor polar components against oxidative damage in rats treated with ferric-nitrilotriacetate. Food Chem Toxicol. 2007;45:2434-40 pubmed
    ..In summary, MPC administration protects organs against lipid peroxidation and conserves the endogenous alpha-tocopherol...
  53. Wijeratne S, Byrne N, Walker K. Separation of alpha- from beta-arylalanines by nickel nitrilotriacetate chromatography. J Sep Sci. 2010;33:1279-82 pubmed publisher
    ..The sample recovery was quantitative (>98%), and the column matrix was very resilient, as demonstrated by consistent separation of the solutes after approximately 100 preparative cycles...
  54. Freund O. Biodistribution and gastrointestinal drug delivery of new lipidic multilamellar vesicles. Drug Deliv. 2001;8:239-44 pubmed
    ..The other tissues accumulated <30% of the dose throughout the duration of the study. On the other hand, oral administration of Spherulites, entrapping111 In-NTA, in fasting rats showed a significant increase of radioactivity in blood...
  55. Mizuno R, Kawabata T, Sutoh Y, Nishida Y, Okada S. Oxidative renal tubular injuries induced by aminocarboxylate-type iron (III) coordination compounds as candidate renal carcinogens. Biometals. 2006;19:675-83 pubmed
    ..Fe(III)-IDA was crystallized, and this was confirmed by X-ray analysis and magnetic susceptibility measurements. These data indicated that Fe(III)-IDA possessed a linear mu-oxo bridged dinuclear iron (III) around neutral pH...
  56. Promnares K, Komenda J, Bumba L, Nebesarova J, Vacha F, Tichy M. Cyanobacterial small chlorophyll-binding protein ScpD (HliB) is located on the periphery of photosystem II in the vicinity of PsbH and CP47 subunits. J Biol Chem. 2006;281:32705-13 pubmed
  57. Liu C, Liang A, Hu M. Protective effects of fucoxanthin against ferric nitrilotriacetate-induced oxidative stress in murine hepatic BNL CL.2 cells. Toxicol In Vitro. 2011;25:1314-9 pubmed publisher
    ..2 cells treated with Fe-NTA, and that the protective effect is likely associated with decreased intracellular ROS, TBARS, protein carbonyl contents and increased GSH levels...
  58. Platt V, Huang Z, Cao L, Tiffany M, Riviere K, Szoka F. Influence of multivalent nitrilotriacetic acid lipid-ligand affinity on the circulation half-life in mice of a liposome-attached His6-protein. Bioconjug Chem. 2010;21:892-902 pubmed publisher
    Metal chelation-ligand interactions, such as occur between nitrilotriacetic acid (NTA)-nickel and multihistidines, enable the noncovalent attachment of histidine-modified proteins to liposomes and other particles...
  59. Kelly D, Dukovski D, Walz T. Monolayer purification: a rapid method for isolating protein complexes for single-particle electron microscopy. Proc Natl Acad Sci U S A. 2008;105:4703-8 pubmed publisher
    ..Here, we introduce the "monolayer purification" method, which employs nickel-nitrilotriacetic acid (Ni-NTA) functionalized lipids for simultaneously purifying His-tagged complexes directly from cell ..
  60. Liu S, Li L, Hao Q, Yin X, Zhang M, Li Q, et al. A novel non-enzymatic hydrogen peroxide sensor based on Mn-nitrilotriacetate acid (Mn-NTA) nanowires. Talanta. 2010;81:727-31 pubmed publisher
    ..5 x 10(-3)M with a detection limit of 2 x 10(-7)M. The sensitivity was up to 78.9 microA mM(-1)cm(-2). These results indicated that the Mn-NTA nanowires were promising in realizing non-enzymatic H(2)O(2) detection...
  61. Govender K, Cukrowski I. Density functional theory and isodesmic reaction based prediction of four stepwise protonation constants, as log KH(n), for nitrilotriacetic acid. The importance of a kind and protonated form of a reference molecule used. J Phys Chem A. 2010;114:1868-78 pubmed publisher
    ..energies in solution from a single continuum model, was used to predict stepwise protonation constants of nitrilotriacetic acid. Calculations were performed at the RB3LYP/6-311+G(d,p) level of theory in conjunction with the PCM-UA0 ..
  62. Hattori T, Okitsu K, Yamazaki N, Ohoka N, Shibata N, Misawa T, et al. Simple and efficient knockdown of His-tagged proteins by ternary molecules consisting of a His-tag ligand, a ubiquitin ligase ligand, and a cell-penetrating peptide. Bioorg Med Chem Lett. 2017;27:4478-4481 pubmed publisher
    ..target protein degradation inducers contained three functional moieties: a His-tag ligand (nickel nitrilotriacetic acid [Ni-NTA]), an E3 ligand (bestatin [BS] or MV1), and a carrier peptide (Tat or nonaarginine [R9])...
  63. Gupta A, Sharma S, Kaur I, Chopra K. Renoprotective effects of sesamol in ferric nitrilotriacetate-induced oxidative renal injury in rats. Basic Clin Pharmacol Toxicol. 2009;104:316-21 pubmed publisher
    ..Present findings strongly suggest the pivotal role of oxidative stress in the ferric nitrilotriacetate-induced renal dysfunction and points towards the renoprotective potential of sesamol in oxidative renal pathologies...
  64. Brooks S, Carroll S. pH-Dependent fate and transport of NTA-complexed cobalt through undisturbed cores of fractured shale saprolite. J Contam Hydrol. 2002;58:191-207 pubmed
  65. Min K, Morishita F, Tetsuchikawahara N, Onosaka S. Induction of hepatic and renal metallothionein synthesis by ferric nitrilotriacetate in mice: the role of MT as an antioxidant. Toxicol Appl Pharmacol. 2005;204:9-17 pubmed
    ..These results indicate that induction of MT synthesis may result from reactive oxygen species (ROS) generated by Fe-NTA, and MT may act in vivo as a complementary antioxidant...
  66. Nomoto M, Yamaguchi R, Kohno K, Kasai H. Relations between clusters of oxidatively damaged nucleotides and active or open nucleosomes in the rat Nth 1 gene. Oncogene. 2002;21:1649-57 pubmed
    ..Taking into account the function of the protein segment encoded by these regions, we discussed the molecular mechanism of the restricted formation of the damaged nucleotides...
  67. Kang S, Shin M, Kim J, Oh J. Proteomic profiling of cellular proteins interacting with the hepatitis C virus core protein. Proteomics. 2005;5:2227-37 pubmed
    ..We discuss the functional implications of the interactions of the core protein with newly identified cellular proteins in HCV infection and pathogenesis...
  68. Kessova I, Cederbaum A. The effect of CYP2E1-dependent oxidant stress on activity of proteasomes in HepG2 cells. J Pharmacol Exp Ther. 2005;315:304-12 pubmed
  69. Yuan Z, VanBriesen J. Bacterial growth yields on EDTA, NTA, and their biodegradation intermediates. Biodegradation. 2008;19:41-52 pubmed
    Ethylenediaminetetraacetic acid (EDTA) and nitrilotriacetic acid (NTA) are widely used anthropogenic chelating agents for control of metal speciation and are ubiquitous in natural waters and wastewaters...
  70. Backmark A, Olivier N, Snijder A, Gordon E, Dekker N, Ferguson A. Fluorescent probe for high-throughput screening of membrane protein expression. Protein Sci. 2013;22:1124-32 pubmed publisher
    ..This probe provides a highly sensitive tool to detect target proteins to expression levels down to 0.02 mg/L in crude lysate, and requires minimal amounts of cell culture. ..
  71. Vaish A, Silin V, Walker M, Steffens K, Krueger S, Yeliseev A, et al. A generalized strategy for immobilizing uniformly oriented membrane proteins at solid interfaces. Chem Commun (Camb). 2013;49:2685-7 pubmed publisher
    ..Using water soluble nitrilotriacetic acid (NTA)-terminated oligo(ethylene glycol) thiols, a histidine-tagged G protein-coupled membrane receptor (..
  72. Kaur G, Jabbar Z, Athar M, Alam M. Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice. Food Chem Toxicol. 2006;44:984-93 pubmed
    ..These results indicate pomegranate flowers to possess potent antioxidant and hepatoprotective property, the former being probably responsible for the latter...
  73. Mason A, Halbrooks P, James N, Byrne S, Grady J, Chasteen N, et al. Structural and functional consequences of the substitution of glycine 65 with arginine in the N-lobe of human transferrin. Biochemistry. 2009;48:1945-53 pubmed publisher
  74. Zhang H, Yang H, Wang C, He H, Ma W, Yang S. [Activity and quality comparison of the engineered protein Staphylococcus aureus alpha-hemolysin purified with gel filtration chromatography and Ni-NTA]. Sheng Wu Gong Cheng Xue Bao. 2009;25:176-80 pubmed
    ..5%, respectively. The results showed that there is no significant difference in the quality, hemolysis activity and yield of the recombinant proteins purified with Ni-NTA spin columns and GFC...
  75. Lone I, Kaur G, Athar M, Alam M. Protective effect of Rumex patientia (English Spinach) roots on ferric nitrilotriacetate (Fe-NTA) induced hepatic oxidative stress and tumor promotion response. Food Chem Toxicol. 2007;45:1821-9 pubmed
    ..The data obtained in the present study illustrates R. patientia roots to possess potent antioxidant and free radical scavenging activities and thwart oxidative damage and hyperproliferation in hepatic tissues...
  76. Zhou J, Dang Z, Chen N, Xu S, Xie Z. [Enhanced phytoextraction of heavy metal contaminated soil by chelating agents and auxin indole-3-acetic acid]. Huan Jing Ke Xue. 2007;28:2085-8 pubmed
    ..Chelating agent ethylenediaminetetraacetic acid (EDTA) and nitrilotriacetic acid (NTA) and auxin indole-3-acetic acid (IAA) were used for enhancing heavy metals uptake from soils by Zea ..
  77. Prasad L, Khan T, Jahangir T, Sultana S. Abrogation of DEN/Fe-NTA induced carcinogenic response, oxidative damage and subsequent cell proliferation response by Terminalia chebula in kidney of Wistar rats. Pharmazie. 2007;62:790-7 pubmed
    ..chebula in the prevention of ferric nitrilotriacetic acid (Fe- NTA) induced oxidative stress and renal tumorigenesis in Wistar rats...
  78. Bresolin I, Borsoi Ribeiro M, Tamashiro W, Augusto E, Vijayalakshmi M, Bueno S. Evaluation of immobilized metal-ion affinity chromatography (IMAC) as a technique for IgG(1) monoclonal antibodies purification: the effect of chelating ligand and support. Appl Biochem Biotechnol. 2010;160:2148-65 pubmed publisher
    ..The chelating ligands iminodiacetic acid, carboxymethylated aspartic acid (CM-Asp), nitrilotriacetic acid, and tris (carboxymethyl) ethylenediamine in agarose gels with immobilized Ni(II) and Zn(II) ions were ..
  79. Pujol A, Gateau C, Lebrun C, Delangle P. A cysteine-based tripodal chelator with a high affinity and selectivity for copper(I). J Am Chem Soc. 2009;131:6928-9 pubmed publisher
    A C(3)-symmetric ligand containing three converging cysteine chains anchored on a nitrilotriacetic acid moiety has been synthesized...
  80. Zhou J, Dang Z, Tao X, Zhou Y. [Influence of NTA on accumulation and subcellular distribution of copper and zinc in corn (Zea mays)]. Huan Jing Ke Xue. 2005;26:126-30 pubmed
    ..stems and leaves of corn (zea mays) growing on multi-metal contaminated soil with the addition of chelator Nitrilotriacetic Acid (NTA)...
  81. Pinheiro J, Salvador J, Companys E, Galceran J, Puy J. Experimental verification of the metal flux enhancement in a mixture of two metal complexes: the Cd/NTA/glycine and Cd/NTA/citric acid systems. Phys Chem Chem Phys. 2010;12:1131-8 pubmed publisher
    ..Theoretical discussion of the flux enhancement factors and of the conditions for this enhancement are also provided...
  82. Mizote A, Hida A, Hosako M, Fujisawa M, Kamekawa M, Okada S. Effects of phlebotomy on the growth of ferric nitrilotriacetate-induced renal cell carcinoma. Acta Med Okayama. 2002;56:199-204 pubmed
    ..In addition, we showed that iron withdrawal at the promotion stage of carcinogenesis will retard tumor growth...