iodoacetates

Summary

Summary: Iodinated derivatives of acetic acid. Iodoacetates are commonly used as alkylating sulfhydryl reagents and enzyme inhibitors in biochemical research.

Top Publications

  1. Combe R, Bramwell S, Field M. The monosodium iodoacetate model of osteoarthritis: a model of chronic nociceptive pain in rats?. Neurosci Lett. 2004;370:236-40 pubmed
    ..In conclusion, the MIA model of OA is reproducible and mimics OA pain in humans. Analgesic drug studies indicate this model may be useful for investigating chronic nociceptive pain...
  2. Piscaer T, Waarsing J, Kops N, Pavljasevic P, Verhaar J, van Osch G, et al. In vivo imaging of cartilage degeneration using microCT-arthrography. Osteoarthritis Cartilage. 2008;16:1011-7 pubmed publisher
    ..In the present study, we investigated the use of micro-computed tomography (microCT) in combination with a negatively charged ionic iodine dimer (ioxaglate) for in vivo assessment of cartilage degeneration in a small animal model...
  3. Cledes G, Felizardo R, Foucart J, Carpentier P. Validation of a chemical osteoarthritis model in rabbit temporomandibular joint: a compliment to biomechanical models. Int J Oral Maxillofac Surg. 2006;35:1026-33 pubmed
    ..This could be interpreted as a repairing attempt due to the specific potential of fibrocartilage proliferative cells...
  4. Pomonis J, Boulet J, Gottshall S, Phillips S, Sellers R, Bunton T, et al. Development and pharmacological characterization of a rat model of osteoarthritis pain. Pain. 2005;114:339-46 pubmed
    ..We conclude that the iodoacetate model of OA is a relevant animal model to study pain associated with OA, and can be used to test potential therapeutic agents...
  5. Hernández Fonseca K, Cárdenas Rodríguez N, Pedraza Chaverri J, Massieu L. Calcium-dependent production of reactive oxygen species is involved in neuronal damage induced during glycolysis inhibition in cultured hippocampal neurons. J Neurosci Res. 2008;86:1768-80 pubmed publisher
  6. Silva A, Andersen M, Tufik S. Sleep pattern in an experimental model of osteoarthritis. Pain. 2008;140:446-55 pubmed publisher
    ..Thus, pain associated with the rat OA model causes alterations in sleep architecture by disrupting the sleep pattern...
  7. Schuelert N, McDougall J. Grading of monosodium iodoacetate-induced osteoarthritis reveals a concentration-dependent sensitization of nociceptors in the knee joint of the rat. Neurosci Lett. 2009;465:184-8 pubmed publisher
    ..The anti-nociceptive effect of diclofenac further indicates that the MIA model offers an attractive means of objectively testing potential therapeutic agents...
  8. Strassle B, Mark L, Leventhal L, Piesla M, Jian Li X, Kennedy J, et al. Inhibition of osteoclasts prevents cartilage loss and pain in a rat model of degenerative joint disease. Osteoarthritis Cartilage. 2010;18:1319-28 pubmed publisher
    ..To investigate the relationship between efficacy of a bisphosphonate, pain and extent of joint damage in the monosodium iodoacetate (MIA) model of painful degenerative joint disease...
  9. Sagar D, Staniaszek L, Okine B, Woodhams S, Norris L, Pearson R, et al. Tonic modulation of spinal hyperexcitability by the endocannabinoid receptor system in a rat model of osteoarthritis pain. Arthritis Rheum. 2010;62:3666-76 pubmed publisher
    ..To investigate the impact of an experimental model of osteoarthritis (OA) on spinal nociceptive processing and the role of the inhibitory endocannabinoid system in regulating sensory processing at the spinal level...

More Information

Publications85

  1. Cárdenas Rodríguez N, Guzmán Beltrán S, Medina Campos O, Orozco Ibarra M, Massieu L, Pedraza Chaverri J. The effect of nordihydroguaiaretic acid on iodoacetate-induced toxicity in cultured neurons. J Biochem Mol Toxicol. 2009;23:137-42 pubmed publisher
    ..Furthermore, NDGA could prevent the inhibitory effect of IAA on aconitase activity, a marker of oxidative stress, suggesting that the protective effect of NDGA on IAA neurotoxicity was associated with the prevention of oxidative stress...
  2. Barve R, Minnerly J, Weiss D, Meyer D, Aguiar D, Sullivan P, et al. Transcriptional profiling and pathway analysis of monosodium iodoacetate-induced experimental osteoarthritis in rats: relevance to human disease. Osteoarthritis Cartilage. 2007;15:1190-8 pubmed
    ..This was accomplished through pathway, network and system level comparisons of transcriptional profiles generated from animal and human disease cartilage...
  3. Kobayashi K, Imaizumi R, Sumichika H, Tanaka H, Goda M, Fukunari A, et al. Sodium iodoacetate-induced experimental osteoarthritis and associated pain model in rats. J Vet Med Sci. 2003;65:1195-9 pubmed
    ..We conclude that the present experimental model in combination with a novel dual channel weight averager would be very useful for the study of human OA, and could be applied for estimation of therapeutic effect of new anti-OA drugs...
  4. Fahim F, Esmat A, Mady E, Ibrahim E. Antitumor activities of iodoacetate and dimethylsulphoxide against solid Ehrlich carcinoma growth in mice. Biol Res. 2003;36:253-62 pubmed
    ..Combined treatment increased significantly the levels of plasma LDH and erythrocytes G6PDH activities, as well as liver glycogen, and in contrast it decreased the levels of liver total protein, RNA and DNA, compared to normal controls...
  5. Janusz M, Hookfin E, Heitmeyer S, Woessner J, Freemont A, Hoyland J, et al. Moderation of iodoacetate-induced experimental osteoarthritis in rats by matrix metalloproteinase inhibitors. Osteoarthritis Cartilage. 2001;9:751-60 pubmed
    ..To determine the effect of matrix metalloproteinase (MMP) inhibitors in mono-iodoacetate-induced arthritis in rats...
  6. Ferreira Gomes J, Adães S, Sarkander J, Castro Lopes J. Phenotypic alterations of neurons that innervate osteoarthritic joints in rats. Arthritis Rheum. 2010;62:3677-85 pubmed publisher
  7. Mohan G, Perilli E, Kuliwaba J, Humphries J, Parkinson I, Fazzalari N. Application of in vivo micro-computed tomography in the temporal characterisation of subchondral bone architecture in a rat model of low-dose monosodium iodoacetate-induced osteoarthritis. Arthritis Res Ther. 2011;13:R210 pubmed publisher
    ..In this study we characterized the temporal changes in the tibial subchondral bone architecture in a rat model of low-dose monosodium iodoacetate (MIA)-induced OA using in vivo micro-computed tomography (CT)...
  8. Thakur M, Rahman W, Hobbs C, Dickenson A, Bennett D. Characterisation of a peripheral neuropathic component of the rat monoiodoacetate model of osteoarthritis. PLoS ONE. 2012;7:e33730 pubmed publisher
    ..This significant neuropathic component needs to be taken into account when interpreting studies using this model, particularly at doses greater than 1 mg MIA. ..
  9. Kalff K, El Mouedden M, Van Egmond J, Veening J, Joosten L, Scheffer G, et al. Pre-treatment with capsaicin in a rat osteoarthritis model reduces the symptoms of pain and bone damage induced by monosodium iodoacetate. Eur J Pharmacol. 2010;641:108-13 pubmed publisher
    ..The present results support the hypothesis that capsaicin-sensitive sensory neurons contribute to bone lesions in the monosodium iodoacetate-induced osteoarthritis rat model...
  10. Guzman R, Evans M, Bove S, Morenko B, Kilgore K. Mono-iodoacetate-induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints: an animal model of osteoarthritis. Toxicol Pathol. 2003;31:619-24 pubmed
    ..This model offers a rapid and minimally invasive method to reproduce OA-like lesions in a rodent species...
  11. Koh Y, Hong S, Kang H, Chung C, Koo K, Chung H, et al. The effects of bone turnover rate on subchondral trabecular bone structure and cartilage damage in the osteoarthritis rat model. Rheumatol Int. 2010;30:1165-71 pubmed publisher
    ..Furthermore, a correlation was found between cartilage destruction severity and subchondral trabecular change in the intra-articular iodoacetate-injected osteoarthritis rat model...
  12. Baragi V, Becher G, Bendele A, Biesinger R, Bluhm H, Boer J, et al. A new class of potent matrix metalloproteinase 13 inhibitors for potential treatment of osteoarthritis: Evidence of histologic and clinical efficacy without musculoskeletal toxicity in rat models. Arthritis Rheum. 2009;60:2008-18 pubmed publisher
    ..This study was undertaken to identify a new class of potent and selective MMP-13 inhibitors that would provide histologic and clinical efficacy without musculoskeletal toxicity...
  13. Harvey V, Dickenson A. Behavioural and electrophysiological characterisation of experimentally induced osteoarthritis and neuropathy in C57Bl/6 mice. Mol Pain. 2009;5:18 pubmed publisher
    ..These approaches uniquely allow the threshold and suprathreshold measures to many modalities to be quantified and so form a basis for improving and expanding transgenic studies...
  14. Morenko B, Bove S, Chen L, Guzman R, Juneau P, Bocan T, et al. In vivo micro computed tomography of subchondral bone in the rat after intra-articular administration of monosodium iodoacetate. Contemp Top Lab Anim Sci. 2004;43:39-43 pubmed
    ..In light of these data, in vivo microCT imaging represents a valuable tool for investigating bone remolding and has the potential to be used for routine, high-throughput analysis and screening of investigation therapeutics...
  15. Nagase H, Kumakura S, Shimada K. Establishment of a novel objective and quantitative method to assess pain-related behavior in monosodium iodoacetate-induced osteoarthritis in rat knee. J Pharmacol Toxicol Methods. 2012;65:29-36 pubmed publisher
    ..Here, we describe the reduction of spontaneous activity in a rat model of OA as an objective and quantifiable behavioral pain that can predict the analgesic activity of a variety of agents following single-dose administration...
  16. Fernihough J, Gentry C, Bevan S, Winter J. Regulation of calcitonin gene-related peptide and TRPV1 in a rat model of osteoarthritis. Neurosci Lett. 2005;388:75-80 pubmed
    ..Therefore, there is a potential role for CGRP and TRPV1 in the manifestation of pain behaviour accompanied by OA changes in the knee in the iodoacetate induced model...
  17. Beyreuther B, Callizot N, Stöhr T. Antinociceptive efficacy of lacosamide in the monosodium iodoacetate rat model for osteoarthritis pain. Arthritis Res Ther. 2007;9:R14 pubmed
    ..Lacosamide was able to inhibit pain at days 3, 7 and 14 after induction of arthritis. This shows that lacosamide is able to reduce pain behavior induced by multiple mechanisms in animals...
  18. Vermeirsch H, Biermans R, Salmon P, Meert T. Evaluation of pain behavior and bone destruction in two arthritic models in guinea pig and rat. Pharmacol Biochem Behav. 2007;87:349-59 pubmed
  19. Bove S, Calcaterra S, Brooker R, Huber C, Guzman R, Juneau P, et al. Weight bearing as a measure of disease progression and efficacy of anti-inflammatory compounds in a model of monosodium iodoacetate-induced osteoarthritis. Osteoarthritis Cartilage. 2003;11:821-30 pubmed
  20. Vonsy J, Ghandehari J, Dickenson A. Differential analgesic effects of morphine and gabapentin on behavioural measures of pain and disability in a model of osteoarthritis pain in rats. Eur J Pain. 2009;13:786-93 pubmed publisher
    ..In terms of translational research, the effects of morphine and gabapentin validate the model and suggest trials of these therapeutic approaches in OA patients...
  21. Ferreira Gomes J, Adães S, Castro Lopes J. Assessment of movement-evoked pain in osteoarthritis by the knee-bend and CatWalk tests: a clinically relevant study. J Pain. 2008;9:945-54 pubmed publisher
    ..The knee-bend and CatWalk tests are effective for evaluating movement-related nociception, a hallmark of clinical OA, which was present throughout the experimental period...
  22. Lee Y, Pai M, Brederson J, Wilcox D, Hsieh G, Jarvis M, et al. Monosodium iodoacetate-induced joint pain is associated with increased phosphorylation of mitogen activated protein kinases in the rat spinal cord. Mol Pain. 2011;7:39 pubmed publisher
    ..Results of these studies support the role of MAPK activation in the progression and maintenance of central sensitization in the MIA-OA experimental pain model. ..
  23. Guingamp C, Gegout Pottie P, Philippe L, Terlain B, Netter P, Gillet P. Mono-iodoacetate-induced experimental osteoarthritis: a dose-response study of loss of mobility, morphology, and biochemistry. Arthritis Rheum. 1997;40:1670-9 pubmed
    ..To characterize the dose-responsiveness of morphologic and biochemical chondral changes relative to mobility in mono-iodoacetate (MIA)-induced osteoarthritis (OA) in rats...
  24. Bar Yehuda S, Rath Wolfson L, del Valle L, Ochaion A, Cohen S, Patoka R, et al. Induction of an antiinflammatory effect and prevention of cartilage damage in rat knee osteoarthritis by CF101 treatment. Arthritis Rheum. 2009;60:3061-71 pubmed publisher
    ..The purpose of this study was to examine the effects of CF101 on the clinical and pathologic manifestations of OA in an experimental animal model...
  25. Ronen S, DiStefano F, McCoy C, Robertson D, Smith T, Al Saffar N, et al. Magnetic resonance detects metabolic changes associated with chemotherapy-induced apoptosis. Br J Cancer. 1999;80:1035-41 pubmed
    ..The 31P MR spectrum of tumours responding to chemotherapy could be modulated by similar effects. ..
  26. Hernández Fonseca K, Massieu L. Disruption of endoplasmic reticulum calcium stores is involved in neuronal death induced by glycolysis inhibition in cultured hippocampal neurons. J Neurosci Res. 2005;82:196-205 pubmed
    ..These results might be relevant to the understanding the mechanisms involved in neuronal damage related to aging and chronic neurodegenerative diseases, which have been associated with decreased glucose metabolism. ..
  27. Levin M, Franklin E, Frangione B, Pras M. The amino acid sequence of a major nonimmunoglobulin component of some amyloid fibrils. J Clin Invest. 1972;51:2773-6 pubmed
    ..The ASF contains no cysteine, does not resemble any known immunoglobulin, and has not been detected as yet in myeloma-associated amyloid. ..
  28. Saada A, Kahane I. Purification and characterization of urease from Ureaplasma urealyticum. Zentralbl Bakteriol Mikrobiol Hyg A. 1988;269:160-7 pubmed
    ..The optimum pH was pH 7-7.5. The urease activity was inhibited by flurofamide, acetohydroxamic acid, N-ethylmaleimide and p-chloromercuribenzoate but not by iodoacetate. ..
  29. Plummer T. Isolation and sequence of peptides at the active center of bovine carboxypeptidase B. J Biol Chem. 1969;244:5246-53 pubmed
  30. Maita T, Morokuma K, Matsuda G. Amino acid sequences of the tryptic peptides from carboxymethylated L-asparaginase from Escherichia coli. Hoppe Seylers Z Physiol Chem. 1979;360:1483-95 pubmed
    ..The amino acid sequences of the peptides were determined by fragmentation with various enzymes and subtractive Edman degradation. ..
  31. Jain R, Joyce P, Molinete M, Halban P, Gorr S. Oligomerization of green fluorescent protein in the secretory pathway of endocrine cells. Biochem J. 2001;360:645-9 pubmed
    ..These results suggest that the oligomerization of EGFP must be considered when the protein is used as a reporter molecule in the secretory pathway. ..
  32. Kohanski R, Heinrikson R. Primary structure of avian hepatic rhodanese. J Protein Chem. 1990;9:369-77 pubmed
    ..Reaction of the enzyme with a 1.4-fold molar excess of [2-14C]iodoacetate led to inactivation of the enzyme and carboxymethylation of Cys-244; this modification was blocked by the substrate thiosulfate...
  33. Fuhrmann G, Fehlau R, Schneider H, Knauf P. The effect of ferricyanide with iodoacetate in calcium-free solution on passive cation permeability in human red blood cells: comparison with the Gardos-effect and with the influence of PCMBS on passive cation permeability. Biochim Biophys Acta. 1989;983:179-85 pubmed
    ..The ferricyanide effect described here does not involve formation of nonspecific channels. The change in Na+ permeability separately from K+ permeability under certain circumstances suggests a more specific effect. ..
  34. Gong K, Shao W, Chen H, Wang Z, Luo Z. Rat model of lumbar facet joint osteoarthritis associated with facet-mediated mechanical hyperalgesia induced by intra-articular injection of monosodium iodoacetate. J Formos Med Assoc. 2011;110:145-52 pubmed publisher
    ..This model might provide a useful tool for further study to ascertain the complex mechanism of facet joint pain. ..
  35. Tao L, Cheung A, Nibau C, Wu H. RAC GTPases in tobacco and Arabidopsis mediate auxin-induced formation of proteolytically active nuclear protein bodies that contain AUX/IAA proteins. Plant Cell. 2005;17:2369-83 pubmed
  36. Liu S, Bhatnagar A, Ansari N, Srivastava S. Identification of the reactive cysteine residue in human placenta aldose reductase. Biochim Biophys Acta. 1993;1164:268-72 pubmed
    ..In both these peptides Cys-298 was the only radiolabeled residue. It is suggested that Cys-298 regulates the kinetic and inhibition properties of the enzyme, but does not participate in catalysis. ..
  37. Menzies F, Cookson M, Taylor R, Turnbull D, Chrzanowska Lightowlers Z, Dong L, et al. Mitochondrial dysfunction in a cell culture model of familial amyotrophic lateral sclerosis. Brain. 2002;125:1522-33 pubmed
    ..Therapeutic measures aimed at protecting mitochondrial respiratory chain function may be useful in SOD1 related familial and possibly other forms of amyotrophic lateral sclerosis. ..
  38. Wang X, Kou X, He D, Zeng M, Meng Z, Bi R, et al. Progression of cartilage degradation, bone resorption and pain in rat temporomandibular joint osteoarthritis induced by injection of iodoacetate. PLoS ONE. 2012;7:e45036 pubmed publisher
  39. Xu M, Akabas M. Identification of channel-lining residues in the M2 membrane-spanning segment of the GABA(A) receptor alpha1 subunit. J Gen Physiol. 1996;107:195-205 pubmed
    ..The ability of the positively charged reagent methanethiosulfonate ethylammonium to reach the level of alpha1 Thr261 suggests that the charge-selectivity filter is at least as cytoplasmic as this residue. ..
  40. Yadav U, Anjaria K, Nairy R, Shirsath K, Desai U, Chaurasia R, et al. Differential killing and radio-modifying effects of iodoacetate in mammalian normal and cancer cells. Radiat Environ Biophys. 2017;56:227-239 pubmed publisher
    ..13, 1.13, and 1.24. In conclusion, differential killing and radiosensitizing effects of IA suggest that it may have potential use as a anticancer agent and radiosensitizer in cancer therapy. ..
  41. Sánchez Aragó M, Cuezva J. The bioenergetic signature of isogenic colon cancer cells predicts the cell death response to treatment with 3-bromopyruvate, iodoacetate or 5-fluorouracil. J Transl Med. 2011;9:19 pubmed publisher
    ..Overall, we suggest that the determination of the bioenergetic signature of colon carcinomas could provide a tool for predicting the therapeutic response to various chemotherapeutic strategies aimed at combating tumor progression. ..
  42. Dimroth P, Walter H, Lynen F. [Biosynthesis of 6-methylsalicylic acid]. Eur J Biochem. 1970;13:98-110 pubmed
  43. Liao S, Ou Y, Chang C, Chen W, Kuan Y, Wang W, et al. Diethylmaleate and iodoacetate in combination caused profound cell death in astrocytes. J Neurochem. 2013;127:271-82 pubmed publisher
  44. Stewart L, Bakker E, Booth I. Energy coupling to K+ uptake via the Trk system in Escherichia coli: the role of ATP. J Gen Microbiol. 1985;131:77-85 pubmed
    ..Using inhibitors and unc mutants we show that Trk is not an ATPase but may be regulated by ATP. Possible mechanisms of energy coupling to Trk are discussed. ..
  45. Schirch L, Slagel S, Barra D, Martini F, Bossa F. Evidence for a sulfhydryl group at the active site of serine transhydroxymethylase. J Biol Chem. 1980;255:2986-9 pubmed
    ..The reactive sulfhydryl group was labeled with [14C]iodoacetate. From a chymotryptic digest, a 14C-containing peptide was isolated and determined to be: His-Pro-Lys-Leu-Ile-Ile-Ala-Gly-Thr-Ser-Cys(Cm)-Tyr. ..
  46. Hadipour Jahromy M, Mozaffari Kermani R. Chondroprotective effects of pomegranate juice on monoiodoacetate-induced osteoarthritis of the knee joint of mice. Phytother Res. 2010;24:182-5 pubmed publisher
    ..No cell proliferation or inflammatory cells were detected in the synovial fluid. The effectiveness of pomegranate juice in improving histopathological damage is emphasized and its chondroprotective effect in vivo highlighted...
  47. Gao G, Fonda M. Identification of an essential cysteine residue in pyridoxal phosphatase from human erythrocytes. J Biol Chem. 1994;269:8234-9 pubmed
    ..Residues 5-12 and 15 of this peptide are identical with a sequence of a yeast alkaline p-nitrophenylphosphatase, and the peptide has little homology with other mammalian phosphatases...
  48. Sniekers Y, Weinans H, van Osch G, van Leeuwen J. Oestrogen is important for maintenance of cartilage and subchondral bone in a murine model of knee osteoarthritis. Arthritis Res Ther. 2010;12:R182 pubmed publisher
    ..We investigated in an ovariectomized mouse model the impact of oestrogen loss and oestrogen supplementation on articular cartilage and subchondral bone in tibia and patella, and assessed bone changes in osteoarthritis development...
  49. Dowling B, Dart A, Matthews S. Chemical arthrodesis of the distal tarsal joints using sodium monoiodoacetate in 104 horses. Aust Vet J. 2004;82:38-42 pubmed
    ..To evaluate chemical arthrodesis using sodium monoiodoacetate for treatment of degenerative joint disease of the tarsometatarsal and distal intertarsal joints...
  50. Swearingen C, Chambers M, Lin C, Marimuthu J, Rito C, Carter Q, et al. A short-term pharmacodynamic model for monitoring aggrecanase activity: injection of monosodium iodoacetate (MIA) in rats and assessment of aggrecan neoepitope release in synovial fluid using novel ELISAs. Osteoarthritis Cartilage. 2010;18:1159-66 pubmed publisher
    ..To develop a short-term in vivo model in rats, with an enzyme-linked immunosorbent assay (ELISA) readout for specific aggrecanase-cleaved aggrecan fragments, to facilitate testing of aggrecanase inhibitors...
  51. Kiedrowski L, Czyz A. Mitochondria buffer sodium-dependent CA2+ influx in cultured cerebellar granule cells. Ann N Y Acad Sci. 2002;976:413-7 pubmed
  52. Raddatz E, Thomas A, Sarre A, Benathan M. Differential contribution of mitochondria, NADPH oxidases, and glycolysis to region-specific oxidant stress in the anoxic-reoxygenated embryonic heart. Am J Physiol Heart Circ Physiol. 2011;300:H820-35 pubmed publisher
    ..The relative contribution of mitochondria and extramitochondrial systems to ROS generation varies from one region to another and throughout reoxygenation. ..
  53. Corbo R, Scacchi R, Mantuano E. Effect of some thiol reagents on erythrocyte adenosine deaminase (ADA) activity. Enzyme. 1988;39:50-3 pubmed
  54. Vega M, Walsh S, Mantle T, Coll M. The three-dimensional structure of Cys-47-modified mouse liver glutathione S-transferase P1-1. Carboxymethylation dramatically decreases the affinity for glutathione and is associated with a loss of electron density in the alphaB-310B region. J Biol Chem. 1998;273:2844-50 pubmed
    ..A detailed kinetic study of the modified enzyme indicates that the carboxymethylation increases the Km for glutathione by 3 orders of magnitude, although the enzyme can function efficiently under saturating conditions...
  55. Jiang L, Li L, Geng C, Gong D, Jiang L, Ishikawa N, et al. Monosodium iodoacetate induces apoptosis via the mitochondrial pathway involving ROS production and caspase activation in rat chondrocytes in vitro. J Orthop Res. 2013;31:364-9 pubmed publisher
    ..Together these observations suggest that the mechanisms of MIA-induced apoptosis are primarily via ROS production and mitochondria-mediated caspase-3 activation in primary rat chondrocytes...
  56. Beauvieux M, Couzigou P, Gin H, Canioni P, Gallis J. Some processes of energy saving and expenditure occurring during ethanol perfusion in the isolated liver of fed rats; a Nuclear Magnetic Resonance study. BMC Physiol. 2004;4:3 pubmed
    ..An attempt to identify the ethanol-induced ATP-consuming pathways was performed using 31P and 13C Nuclear Magnetic Resonance...
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    ..brevis, although this lactobacillus lacked a functional phaosphoenolpyruvate-dependent phosphortransferase system for glucose, 2-deoxyglucose, or TMG...
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  61. Janusz M, Hookfin E, Brown K, Hsieh L, Heitmeyer S, Taiwo Y, et al. Comparison of the pharmacology of hydroxamate- and carboxylate-based matrix metalloproteinase inhibitors (MMPIs) for the treatment of osteoarthritis. Inflamm Res. 2006;55:60-5 pubmed
    ..Hydroxamic-and carboxylic-acid based matrix metalloproteinase inhibitors (MMPIs) were compared for their potency against various MMPs, pharmacodynamic properties and in vivo efficacy in a model of cartilage degeneration...
  62. Kajikawa H, Amari M, Masaki S. Glucose transport by mixed ruminal bacteria from a cow. Appl Environ Microbiol. 1997;63:1847-51 pubmed
    ..The high-affinity system was sensitive to a decrease in pH (< 6.5) and was inhibited by the presence of sucrose, mannose, and fructose...
  63. Bountra C, Kaila K, Vaughan Jones R. Mechanism of rate-dependent pH changes in the sheep cardiac Purkinje fibre. J Physiol. 1988;406:483-501 pubmed
    ..7. The half-time of pHi recovery from the frequency-dependent acidosis was consistently shorter than that from an intracellular acid load induced by adding and then removing external NH4Cl (10 mmol l-1).(ABSTRACT TRUNCATED AT 400 WORDS)..
  64. Ola M, Tabish M, Khan F, Banu N. Purification and some properties of galectin-1 derived from water buffalo (Bubalus bubalis) brain. Cell Biol Int. 2007;31:578-85 pubmed
    ..Our findings based on physico-chemical properties, carbohydrate and blood group specificities of the galectin may have future implications in biological and clinical applications...
  65. Serban M, Prestwich G. Synthesis of hyaluronan haloacetates and biology of novel cross-linker-free synthetic extracellular matrix hydrogels. Biomacromolecules. 2007;8:2821-8 pubmed
    ..Taken together, the HA haloacetates are attractive candidates for producing cross-linker-free sECM biomaterials that can function either as anti-adhesive barriers or as cytoadhesive sECMs for cell culture in pseudo-3-D...
  66. Kim J, Kim T, Park S, Kim H, Kim S, Lee S, et al. Protective effects of human placenta extract on cartilage degradation in experimental osteoarthritis. Biol Pharm Bull. 2010;33:1004-10 pubmed
    ..HPE inhibited MMP-2 activity in MG-63 cells. MMP-2 and -9 activities were also reduced in the cartilages of HPE-treated knee joints. Our results indicate that HPE has therapeutic effects on OA by protecting cartilage...
  67. Bonner W, Bloch K. Purification and properties of fatty acyl thioesterase I from Escherichia coli. J Biol Chem. 1972;247:3123-33 pubmed
  68. Russell E, McFarland E. Genetics of mouse hemoglobins. Ann N Y Acad Sci. 1974;241:25-38 pubmed
  69. Mengelers H, Maikoe T, Raaijmakers J, Lammers J, Koenderman L. Cognate interaction between human lymphocytes and eosinophils is mediated by beta 2-integrins and very late antigen-4. J Lab Clin Med. 1995;126:261-8 pubmed
    ..The cells interacted with each other provided that an intact cellular metabolism was present--that is, no interaction was seen after treatment with the glycolysis inhibitor sodium mono-iodoacetate.(ABSTRACT TRUNCATED AT 250 WORDS)..
  70. Fletcher D, Qiao M, Smith D. The relationship of raw broiler breast meat color and pH to cooked meat color and pH. Poult Sci. 2000;79:784-8 pubmed
    ..Moreover, cooked meat lightness is more closely associated with raw breast meat pH than with cooked meat pH...
  71. Knaust E, Porwit MacDonald A, Gruber A, Xu D, Peterson C. Different effects of metabolic inhibitors and cyclosporin A on daunorubicin transport in leukemia cells from patients with AML. Leuk Res. 2003;27:183-91 pubmed
    ..Our results imply that other factors than P-glycoprotein (Pgp) are of major importance for in vitro Dnr accumulation in AML blasts and that the role of Pgp as a drug efflux pump is not conclusive...
  72. Bersinger N, Malek A, Benz B, Keller P, Schneider H. Effect of protein synthesis inhibitors and metabolic blockers on the production of placental proteins by the in vitro perfused human placenta. Gynecol Obstet Invest. 1988;25:145-51 pubmed
    ..It is concluded that the above four proteins are synthesised de novo by the perfused placenta in the absence of maternal tissue and that this synthesis is energy-dependent...
  73. Nimmo H, Douglas F, Kleanthous C, Campbell D, Mackintosh C. Identification of a cysteine residue at the active site of Escherichia coli isocitrate lyase. Biochem J. 1989;261:431-5 pubmed
    ..The reactivity of isocitrate lyase to iodoacetate declined with pH, following a titration curve for a group of pKa 7.1. The Km of the enzyme for isocritrate declined over the same pH range...
  74. Miller R, Sies H, Park E, Thomas J. Phosphorylase and creatine kinase modification by thiol-disulfide exchange and by xanthine oxidase-initiated S-thiolation. Arch Biochem Biophys. 1990;276:355-63 pubmed
    ..For proteins typified by phosphorylase b, the accumulation of S-thiolated forms may be more independent of GSH...
  75. McLeese J, Wright G, Youson J, Eales J. Deiodination activity in extrathyroidal tissues of the atlantic hagfish, Myxine glutinosa. J Exp Zool. 2000;287:445-52 pubmed
    ..This contrasts with the situation in teleosts but resembles that reported recently in larval and adult lampreys, suggesting the intestine as a primary site of TH deiodination in lower craniates...
  76. Fontan E, Truffa Bachi P. The threonine-sensitive homoserine dehydrogenase and aspartokinase activities of Escherichia coli K12. Carboxymethylation of the enzyme: threonine binding and inhibition are functionally dissociable. J Biol Chem. 1978;253:2754-7 pubmed
    ..During the carboxymethylation, the protein remains in the tetrameric form until half of the kinase activity is lost. At the end of the inactivation aggregate forms and dimers appear...