butanols

Summary

Summary: Isomeric forms and derivatives of butanol (C4H9OH).

Top Publications

  1. Jones S, Tracy B, Gaida S, Papoutsakis E. Inactivation of ?F in Clostridium acetobutylicum ATCC 824 blocks sporulation prior to asymmetric division and abolishes ?E and ?G protein expression but does not block solvent formation. J Bacteriol. 2011;193:2429-40 pubmed publisher
    ..acetobutylicum chromosome--with the aim of altering cell metabolism and differentiation...
  2. Blombach B, Riester T, Wieschalka S, Ziert C, Youn J, Wendisch V, et al. Corynebacterium glutamicum tailored for efficient isobutanol production. Appl Environ Microbiol. 2011;77:3300-10 pubmed publisher
    ..4 mM h?¹, and showed an overall Y(P/S) of about 0.48 mol per mol of glucose in the production phase...
  3. Bastian S, Liu X, Meyerowitz J, Snow C, Chen M, Arnold F. Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli. Metab Eng. 2011;13:345-52 pubmed publisher
    ..Our results show how engineering cofactor dependence can overcome a critical obstacle to next-generation biofuel commercialization...
  4. Atsumi S, Wu T, Machado I, Huang W, Chen P, Pellegrini M, et al. Evolution, genomic analysis, and reconstruction of isobutanol tolerance in Escherichia coli. Mol Syst Biol. 2010;6:449 pubmed publisher
    ..Interestingly, improved isobutanol tolerance did not increase the final titer of isobutanol production...
  5. Jang Y, Lee J, Malaviya A, Seung D, Cho J, Lee S. Butanol production from renewable biomass: rediscovery of metabolic pathways and metabolic engineering. Biotechnol J. 2012;7:186-98 pubmed publisher
    ..Herein, we review recent discoveries of metabolic pathways for butanol production and the metabolic engineering strategies being developed...
  6. Haus S, Jabbari S, Millat T, Janssen H, Fischer R, Bahl H, et al. A systems biology approach to investigate the effect of pH-induced gene regulation on solvent production by Clostridium acetobutylicum in continuous culture. BMC Syst Biol. 2011;5:10 pubmed publisher
    ..acetobutylicum to be optimized and exploited on an industrial scale, the effect of pH-induced gene regulation on solvent production by C. acetobutylicum in continuous culture must be understood as fully as possible...
  7. Green E. Fermentative production of butanol--the industrial perspective. Curr Opin Biotechnol. 2011;22:337-43 pubmed publisher
    ..This review describes re-commercialization efforts and highlights developments in feedstock utilization, microbial strain development and fermentation process development, all of which significantly impact production costs...
  8. Lehmann D, Lütke Eversloh T. Switching Clostridium acetobutylicum to an ethanol producer by disruption of the butyrate/butanol fermentative pathway. Metab Eng. 2011;13:464-73 pubmed publisher
    ..Further sugar addition in glucose fed-batch fermentations increased the ethanol production to a final titer of 33g/l, resulting in an ethanol to glucose yield of 0.38g/g...
  9. Trinh C, Li J, Blanch H, Clark D. Redesigning Escherichia coli metabolism for anaerobic production of isobutanol. Appl Environ Microbiol. 2011;77:4894-904 pubmed publisher
    ..The glycolytic flux can be controlled to regulate the ratio of isobutanol to ethanol production...

More Information

Publications62

  1. Dellomonaco C, Clomburg J, Miller E, Gonzalez R. Engineered reversal of the ?-oxidation cycle for the synthesis of fuels and chemicals. Nature. 2011;476:355-9 pubmed publisher
    ..The ubiquitous nature of ?-oxidation, aldehyde/alcohol dehydrogenase and thioesterase enzymes has the potential to enable the efficient synthesis of these products in other industrial organisms...
  2. Xiao H, Gu Y, Ning Y, Yang Y, Mitchell W, Jiang W, et al. Confirmation and elimination of xylose metabolism bottlenecks in glucose phosphoenolpyruvate-dependent phosphotransferase system-deficient Clostridium acetobutylicum for simultaneous utilization of glucose, xylose, and arabinose. Appl Environ Microbiol. 2011;77:7886-95 pubmed publisher
    ..06 g/liter) and a 5% higher yield (0.28 g/g) compared to those of the wild-type strain. This strain will be a promising platform host toward commercial exploitation of lignocellulose to produce solvents and biofuels...
  3. Hou S, Jones S, Choe L, Papoutsakis E, Lee K. Workflow for quantitative proteomic analysis of Clostridium acetobutylicum ATCC 824 using iTRAQ tags. Methods. 2013;61:269-76 pubmed publisher
    ..Sixty-one proteins were found to have statistically lower expression levels in stressed versus non-stressed cells. This method may be applicable to other Gram-positive organisms. ..
  4. Millat T, Janssen H, Thorn G, King J, Bahl H, Fischer R, et al. A shift in the dominant phenotype governs the pH-induced metabolic switch of Clostridium acetobutylicumin phosphate-limited continuous cultures. Appl Microbiol Biotechnol. 2013;97:6451-66 pubmed publisher
    ..In particular, the behaviour of AdhE1 and AdhE2 seems to be the key factor for the product formation of the two phenotypes, their pH-dependent growth, and thus, the pH-induced metabolic switch in C. acetobutylicum. ..
  5. Milne C, Eddy J, Raju R, Ardekani S, Kim P, Senger R, et al. Metabolic network reconstruction and genome-scale model of butanol-producing strain Clostridium beijerinckii NCIMB 8052. BMC Syst Biol. 2011;5:130 pubmed publisher
    ..Interrogating C. beijerinckii metabolism from a systems viewpoint using constraint-based modeling allows for simulation of the global effect of genetic modifications...
  6. Lee J, Jang Y, Choi S, Im J, Song H, Cho J, et al. Metabolic engineering of Clostridium acetobutylicum ATCC 824 for isopropanol-butanol-ethanol fermentation. Appl Environ Microbiol. 2012;78:1416-23 pubmed publisher
    ..The resulting PJC4BK(pIPA3-Cm2) strain produced 20.4 g/liter of total alcohol. Fermentation could be prolonged by in situ removal of solvents by gas stripping, and 35.6 g/liter of the IBE mixture could be produced in 45 h...
  7. Janssen H, Döring C, Ehrenreich A, Voigt B, Hecker M, Bahl H, et al. A proteomic and transcriptional view of acidogenic and solventogenic steady-state cells of Clostridium acetobutylicum in a chemostat culture. Appl Microbiol Biotechnol. 2010;87:2209-26 pubmed publisher
    ..Thus, a first systematic study of acidogenic and solventogenic chemostat cultures is presented, and similarities as well as differences to previous studies of batch cultures are discussed...
  8. Higashide W, Li Y, Yang Y, Liao J. Metabolic engineering of Clostridium cellulolyticum for production of isobutanol from cellulose. Appl Environ Microbiol. 2011;77:2727-33 pubmed publisher
    ..Specifically, we have demonstrated the first production of isobutanol to approximately 660 mg/liter from crystalline cellulose by using this microorganism...
  9. Lütke Eversloh T, Bahl H. Metabolic engineering of Clostridium acetobutylicum: recent advances to improve butanol production. Curr Opin Biotechnol. 2011;22:634-47 pubmed publisher
  10. Cai X, Bennett G. Improving the Clostridium acetobutylicum butanol fermentation by engineering the strain for co-production of riboflavin. J Ind Microbiol Biotechnol. 2011;38:1013-25 pubmed publisher
    ..acetobutylicum ATCC 824(pJpGN) could accumulate more than 70 mg/l riboflavin while producing 190 mM butanol in static cultures. Riboflavin production was shown to exert no effect on solvent production at these levels...
  11. Grimmler C, Janssen H, Krausse D, Fischer R, Bahl H, Dürre P, et al. Genome-wide gene expression analysis of the switch between acidogenesis and solventogenesis in continuous cultures of Clostridium acetobutylicum. J Mol Microbiol Biotechnol. 2011;20:1-15 pubmed publisher
    ..The genes for stress response are only induced during the shift but not in the course of solventogenesis when butanol is present in the culture. Finally, the data clearly indicate that solventogenesis is independent from sporulation...
  12. Mao S, Luo Y, Zhang T, Li J, Bao G, Zhu Y, et al. Proteome reference map and comparative proteomic analysis between a wild type Clostridium acetobutylicum DSM 1731 and its mutant with enhanced butanol tolerance and butanol yield. J Proteome Res. 2010;9:3046-61 pubmed publisher
    ..acetobutylicum. The fundamental proteomic data and analyses will be useful for further elucidating the biological mechanism of butanol tolerance and/or enhanced butanol production...
  13. Li S, Wen J, Jia X. Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression. Appl Microbiol Biotechnol. 2011;91:577-89 pubmed publisher
    ..Under microaerobic condition, the engineered B. subtilis produced up to 2.62 g/L isobutanol in shake-flask fed-batch fermentation, which was 21.3% higher than that in batch fermentation...
  14. Tracy B, Jones S, Papoutsakis E. Inactivation of ?E and ?G in Clostridium acetobutylicum illuminates their roles in clostridial-cell-form biogenesis, granulose synthesis, solventogenesis, and spore morphogenesis. J Bacteriol. 2011;193:1414-26 pubmed publisher
    ..Lastly, the sigG inactivation mutant did produce granulose and exhibited wild-type-like solventogenesis...
  15. Amador Noguez D, Brasg I, Feng X, Roquet N, Rabinowitz J. Metabolome remodeling during the acidogenic-solventogenic transition in Clostridium acetobutylicum. Appl Environ Microbiol. 2011;77:7984-97 pubmed publisher
    ..Thus, the solventogenic transition involves global remodeling of metabolism to redirect resources (carbon and reducing power) from biomass production into solvent production...
  16. Wietzke M, Bahl H. The redox-sensing protein Rex, a transcriptional regulator of solventogenesis in Clostridium acetobutylicum. Appl Microbiol Biotechnol. 2012;96:749-61 pubmed publisher
    ..The results presented here clearly indicated that Rex is involved in the redox-dependent solventogenic shift of C. acetobutylicum...
  17. Hönicke D, Janssen H, Grimmler C, Ehrenreich A, Lütke Eversloh T. Global transcriptional changes of Clostridium acetobutylicum cultures with increased butanol:acetone ratios. N Biotechnol. 2012;29:485-93 pubmed publisher
    ..The data presented here indicate a transcriptional regulation for decreased acetone biosynthesis and the redox-dependent substitution of adhE1 (CAP0162) by adhE2...
  18. Kondo T, Tezuka H, Ishii J, Matsuda F, Ogino C, Kondo A. Genetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production from glucose by Saccharomyces cerevisiae. J Biotechnol. 2012;159:32-7 pubmed publisher
    ..cerevisiae was elevated 13-fold, from 11 mg/l to 143 mg/l, and the yield was 6.6 mg/g glucose, which is higher than any previously reported value for S. cerevisiae...
  19. Keis S, Shaheen R, Jones D. Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. nov. and Clostridium saccharobutylicum sp. nov. Int J Syst Evol Microbiol. 2001;51:2095-103 pubmed
    ..The names Clostridium saccharoperbutylacetonicum sp. nov. [type strain = N1-4 (HMT) = ATCC 27021T] and Clostridium saccharobutylicum sp. nov. (type strain = DSM 13864T = ATCC BAA-117T) are proposed for the two new species...
  20. Alsaker K, Paredes C, Papoutsakis E. Metabolite stress and tolerance in the production of biofuels and chemicals: gene-expression-based systems analysis of butanol, butyrate, and acetate stresses in the anaerobe Clostridium acetobutylicum. Biotechnol Bioeng. 2010;105:1131-47 pubmed publisher
  21. Dürre P. Biobutanol: an attractive biofuel. Biotechnol J. 2007;2:1525-34 pubmed
    ..Its genome has been sequenced, and the regulation of solvent formation is under intensive investigation. This opens the possibility to engineer recombinant strains with superior biobutanol-producing ability...
  22. Fontaine L, Meynial Salles I, Girbal L, Yang X, Croux C, Soucaille P. Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824. J Bacteriol. 2002;184:821-30 pubmed
    ..acetobutylicum ATCC 824, and to our knowledge this is the first example of a bacterium with two AADHs. It is noteworthy that the two corresponding genes, adhE and adhE2, are carried by the pSOL1 megaplasmid of C. acetobutylicum ATCC 824...
  23. Gheshlaghi R, Scharer J, Moo Young M, Chou C. Metabolic pathways of clostridia for producing butanol. Biotechnol Adv. 2009;27:764-81 pubmed publisher
  24. Zhao Y, Tomas C, Rudolph F, Papoutsakis E, Bennett G. Intracellular butyryl phosphate and acetyl phosphate concentrations in Clostridium acetobutylicum and their implications for solvent formation. Appl Environ Microbiol. 2005;71:530-7 pubmed
    ..DNA array-based transcriptional analysis of the buk and M5 mutants demonstrated that high BuP levels corresponded to downregulation of flagellar genes and upregulation of solvent formation and stress genes...
  25. Berezina O, Brandt A, Yarotsky S, Schwarz W, Zverlov V. Isolation of a new butanol-producing Clostridium strain: high level of hemicellulosic activity and structure of solventogenesis genes of a new Clostridium saccharobutylicum isolate. Syst Appl Microbiol. 2009;32:449-59 pubmed publisher
    ..saccharobutylicum Ox29 were most similar to those of Clostridium beijerinckii. The activity of some of the bcs-operon genes, expressed in heterologous E. coli, was determined...
  26. Qureshi N, Ezeji T, Ebener J, Dien B, Cotta M, Blaschek H. Butanol production by Clostridium beijerinckii. Part I: use of acid and enzyme hydrolyzed corn fiber. Bioresour Technol. 2008;99:5915-22 pubmed
    ..beijerinckii BA101. In our fermentations, an ABE yield ranging from 0.35 to 0.39 was obtained, which is higher than reported by the other investigators...
  27. Chiao J, Sun Z. History of the acetone-butanol-ethanol fermentation industry in China: development of continuous production technology. J Mol Microbiol Biotechnol. 2007;13:12-4 pubmed
    ..Due to the tremendous national demand for solvents, China has begun a new round of ABE fermentation research. It is expected that a new era in the ABE industry is on the horizon...
  28. Ezeji T, Karcher P, Qureshi N, Blaschek H. Improving performance of a gas stripping-based recovery system to remove butanol from Clostridium beijerinckii fermentation. Bioprocess Biosyst Eng. 2005;27:207-14 pubmed
    ..The productivity was reduced as a result of addition of an excessive amount of antifoam used to inhibit the production of foam caused by the smaller bubbles. This suggested that the fermentation was negatively affected by antifoam...
  29. Qureshi N, Hughes S, Maddox I, Cotta M. Energy-efficient recovery of butanol from model solutions and fermentation broth by adsorption. Bioprocess Biosyst Eng. 2005;27:215-22 pubmed
    ..Other techniques such as gas stripping and pervaporation require 5,220 and 3,295 kcal kg(-1) butanol, respectively...
  30. Klein Marcuschamer D, Santos C, Yu H, Stephanopoulos G. Mutagenesis of the bacterial RNA polymerase alpha subunit for improvement of complex phenotypes. Appl Environ Microbiol. 2009;75:2705-11 pubmed publisher
    ..In addition, we explore the underlying physiological changes that give rise to the solvent-tolerant mutant...
  31. Ezeji T, Qureshi N, Blaschek H. Acetone butanol ethanol (ABE) production from concentrated substrate: reduction in substrate inhibition by fed-batch technique and product inhibition by gas stripping. Appl Microbiol Biotechnol. 2004;63:653-8 pubmed
    ..A total of 500 g glucose was used to produce 232.8 g solvents (77.7 g acetone, 151.7 g butanol, 3.4 g ethanol) in 1 l culture broth. The average solvent yield and productivity were 0.47 g g(-1) and 1.16 g l(-1) h(-1), respectively...
  32. Zhao Y, Hindorff L, Chuang A, Monroe Augustus M, Lyristis M, Harrison M, et al. Expression of a cloned cyclopropane fatty acid synthase gene reduces solvent formation in Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol. 2003;69:2831-41 pubmed
    ..The overexpression of a marR homologous gene preceding the cfa gene in the clostridial genome resulted in reduced cyclopropane fatty acid accumulation...
  33. Smith K, Cho K, Liao J. Engineering Corynebacterium glutamicum for isobutanol production. Appl Microbiol Biotechnol. 2010;87:1045-55 pubmed publisher
    ..9 g/L isobutanol in a pycldh background. These results show promise in engineering C. glutamicum for higher chain alcohol production using the 2-keto acid pathways...
  34. Alsaker K, Spitzer T, Papoutsakis E. Transcriptional analysis of spo0A overexpression in Clostridium acetobutylicum and its effect on the cell's response to butanol stress. J Bacteriol. 2004;186:1959-71 pubmed
  35. Van der Mey M, Janssen C, Janssens F, Jurzak M, Langlois X, Sommen F, et al. Synthesis and biodistribution of [(11)C]R116301, a promising PET ligand for central NK(1) receptors. Bioorg Med Chem. 2005;13:1579-86 pubmed
    ..33 and 1.62 was also observed for olfactory bulb and olfactory tubercules, respectively (20 min p.i.). In summary, [(11)C]R116301 appears to be a promising radioligand suitable for the visualization of NK(1) receptors in vivo using PET...
  36. Schaffer S, Isci N, Zickner B, Dürre P. Changes in protein synthesis and identification of proteins specifically induced during solventogenesis in Clostridium acetobutylicum. Electrophoresis. 2002;23:110-21 pubmed
    ..The proteins acetoacetate decarboxylase and Hsp 18 occurred in two variants, indicating possible covalent modification of these proteins...
  37. Atsumi S, Wu T, Eckl E, Hawkins S, Buelter T, Liao J. Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genes. Appl Microbiol Biotechnol. 2010;85:651-7 pubmed publisher
    ..coli harboring the isobutanol synthesis pathway. Overexpression of yqhD or adhA in E. coli showed better production than ADH2, a result confirmed by activity measurements with isobutyraldehyde...
  38. Gapes J. The economics of acetone-butanol fermentation: theoretical and market considerations. J Mol Microbiol Biotechnol. 2000;2:27-32 pubmed
    ..The following analysis is intended to help identify suitable niche markets...
  39. Dürre P. Fermentative butanol production: bulk chemical and biofuel. Ann N Y Acad Sci. 2008;1125:353-62 pubmed publisher
    ..Two major companies have already committed themselves to biobutanol production as a biofuel additive. Thus, butanol fermentation is on the rise again...
  40. Connor M, Liao J. Microbial production of advanced transportation fuels in non-natural hosts. Curr Opin Biotechnol. 2009;20:307-15 pubmed publisher
    ..The isobutanol production process, in particular, has already reached near-industrial level. Although many of these processes are still being scaled-up for commercial production, their potential for practical application is promising...
  41. Zheng Y, Li L, Xian M, Ma Y, Yang J, Xu X, et al. Problems with the microbial production of butanol. J Ind Microbiol Biotechnol. 2009;36:1127-38 pubmed publisher
    ..Gas stripping is the best technique for butanol recovery found so far...
  42. Inui M, Suda M, Kimura S, Yasuda K, Suzuki H, Toda H, et al. Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli. Appl Microbiol Biotechnol. 2008;77:1305-16 pubmed
    ..This study reports the novel butanol production by an aerobically pregrown microorganism possessing the genes of a strict anaerobe, Clostridium acetobutylicum...
  43. Sillers R, Al Hinai M, Papoutsakis E. Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations. Biotechnol Bioeng. 2009;102:38-49 pubmed publisher
  44. Shi Z, Blaschek H. Transcriptional analysis of Clostridium beijerinckii NCIMB 8052 and the hyper-butanol-producing mutant BA101 during the shift from acidogenesis to solventogenesis. Appl Environ Microbiol. 2008;74:7709-14 pubmed publisher
  45. Nielsen D, Leonard E, Yoon S, Tseng H, Yuan C, Prather K. Engineering alternative butanol production platforms in heterologous bacteria. Metab Eng. 2009;11:262-73 pubmed publisher
    ..putida was dependent upon expression of bcd-etfAB. These results demonstrate the potential of engineering butanol biosynthesis in a variety of heterologous microorganisms, including those cultivated aerobically...
  46. Brynildsen M, Liao J. An integrated network approach identifies the isobutanol response network of Escherichia coli. Mol Syst Biol. 2009;5:277 pubmed publisher
    ..The network described here could aid design and comprehension of alcohol tolerance, whereas the approach provides a general framework to characterize complex phenomena at the systems level...
  47. Dürre P, Böhringer M, Nakotte S, Schaffer S, Thormann K, Zickner B. Transcriptional regulation of solventogenesis in Clostridium acetobutylicum. J Mol Microbiol Biotechnol. 2002;4:295-300 pubmed
    ..The recent completion of C. acetobutylicum genome sequencing will pave the way for transcriptional profiling and thus allow comprehension of the coherent regulatory networks of solventogenesis and sporulation...
  48. Lou W, Chen L, Zhang B, Smith T, Zong M. Using a water-immiscible ionic liquid to improve asymmetric reduction of 4-(trimethylsilyl)-3-butyn-2-one catalyzed by immobilized Candida parapsilosis CCTCC M203011 cells. BMC Biotechnol. 2009;9:90 pubmed publisher
  49. Lee J, Jang Y, Lee J, Papoutsakis E, Lee S. Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production. Biotechnol J. 2009;4:1432-40 pubmed publisher
    ..A plausible reason for this phenomenon is that the cellular metabolism was shifted towards acetate production to compensate reduced ATP production during the largely growth-associated butanol formation by the M5(pIMP1E1AB) strain...
  50. Atsumi S, Hanai T, Liao J. Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. Nature. 2008;451:86-9 pubmed publisher
    ..In particular, we have achieved high-yield, high-specificity production of isobutanol from glucose. The strategy enables the exploration of biofuels beyond those naturally accumulated to high quantities in microbial fermentation...
  51. Ni Y, Sun Z. Recent progress on industrial fermentative production of acetone-butanol-ethanol by Clostridium acetobutylicum in China. Appl Microbiol Biotechnol. 2009;83:415-23 pubmed publisher
    ..Challenges for an economically competitive ABE process in the future are also discussed...
  52. Ezeji T, Qureshi N, Blaschek H. Butanol fermentation research: upstream and downstream manipulations. Chem Rec. 2004;4:305-14 pubmed
  53. Jiang Y, Xu C, Dong F, Yang Y, Jiang W, Yang S. Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio. Metab Eng. 2009;11:284-91 pubmed publisher
    ..8%. This study presents a general method of blocking acetone production by Clostridium and demonstrates the industrial potential of strain 2018adc...