nuclear transfer techniques
Summary: Methods of implanting a CELL NUCLEUS from a donor cell into an enucleated acceptor cell.
Wang P, Li X, Cao L, Huang S, Li H, Zhang Y, et al
. MicroRNA-148a overexpression improves the early development of porcine somatic cell nuclear transfer embryos. PLoS ONE. 2017;12:e0180535 pubmed publisher
..These results indicate that miR-148a overexpression enhances the developmental potential of SCNT embryos and modifies epigenetic status. ..
Yu M, Muteti C, Ogugo M, Ritchie W, Raper J, Kemp S. Cloning of the African indigenous cattle breed Kenyan Boran. Anim Genet. 2016;47:510-1 pubmed publisher
Sim B, Park C, Kang M, Min K. Abnormal gene expression in regular and aggregated somatic cell nuclear transfer placentas. BMC Biotechnol. 2017;17:34 pubmed publisher
..Taken together, these results suggest that abnormal gene expression in cloned placentas was reduced in a genome-wide manner using the aggregation method with tetraploid embryos. ..
Shen K, Li X, Dai X, Wang P, Li S, Xiong Z, et al
. Effects of MG132 on the in vitro development and epigenetic modification of Debao porcine somatic cell nuclear transfer embryos. Theriogenology. 2017;94:48-58 pubmed publisher
..These results demonstrate that MG132 treatment can improve the developmental potential of Debao porcine SCNT embryos through regulating the expression of genes related to histone acetylation and the processes of ZGA. ..
Clancy Thompson E, Chen G, Tyler P, Servos M, Barisa M, Brennan P, et al
. Monoclonal Invariant NKT (iNKT) Cell Mice Reveal a Role for Both Tissue of Origin and the TCR in Development of iNKT Functional Subsets. J Immunol. 2017;199:159-171 pubmed publisher
..Although each individual monoclonal TCR showed an inherent subset distribution preference that was evident across all tissues examined, the iNKT cytokine profile differed more by tissue of origin than by TCR specificity. ..
Lee S, Hyun H, Park M, Choi Y, Son Y, Park Y, et al
. Production of transgenic pig as an Alzheimer's disease model using a multi-cistronic vector system. PLoS ONE. 2017;12:e0177933 pubmed publisher
..Five or more copies of transgenes were inserted into chromosome X. This is the first report of an AD Tg pig derived from a multi-cistronic vector. ..
Zhang J, Qu P, Zhou C, Liu X, Ma X, Wang M, et al
. MicroRNA-125b is a key epigenetic regulatory factor that promotes nuclear transfer reprogramming. J Biol Chem. 2017;292:15916-15926 pubmed publisher
..We conclude that miR-125b promotes SCNT-mediated nuclear reprogramming by targeting SUV39H1 to decrease the deposition of repressive H3K9me3 modifications. ..
Poulton J, Oakeshott P. Nuclear transfer to prevent maternal transmission of mitochondrial DNA disease. BMJ. 2012;345:e6651 pubmed publisher
Jin J, Lee S, Khoirinaya C, Oh A, Kim G, Lee B. Supplementation with spermine during in vitro maturation of porcine oocytes improves early embryonic development after parthenogenetic activation and somatic cell nuclear transfer. J Anim Sci. 2016;94:963-70 pubmed publisher
..In conclusion, 10 µM spermine supplementation during IVM improved the development of porcine PA and SCNT embryos by increasing intracellular GSH, scavenging ROS levels, and regulating gene expression. ..