electron microscope tomography

Summary

Summary: A tomographic technique for obtaining 3-dimensional images with transmission electron microscopy.

Top Publications

  1. Higgs H. Discussing the morphology of actin filaments in lamellipodia. Trends Cell Biol. 2011;21:2-4; author reply 4-5 pubmed publisher
  2. Kieffer C, Ladinsky M, Ninh A, Galimidi R, Bjorkman P. Longitudinal imaging of HIV-1 spread in humanized mice with parallel 3D immunofluorescence and electron tomography. elife. 2017;6: pubmed publisher
    ..These studies highlight the importance of multiscale imaging of HIV-1-infected tissues and are adaptable to other animal models and human patient samples. ..
  3. Atherton J, Jiang K, Stangier M, Luo Y, Hua S, Houben K, et al. A structural model for microtubule minus-end recognition and protection by CAMSAP proteins. Nat Struct Mol Biol. 2017;24:931-943 pubmed publisher
    ..The steric clash between microtubule-bound CKK and kinesin motors explains how CKK protects microtubule minus ends against kinesin-13-induced depolymerization and thus controls the stability of free microtubule minus ends. ..
  4. Chen M, Dai W, Sun S, Jonasch D, He C, Schmid M, et al. Convolutional neural networks for automated annotation of cellular cryo-electron tomograms. Nat Methods. 2017;14:983-985 pubmed publisher
    ..Subsequent subtomogram classification and averaging yield in situ structures of molecular components of interest. The method is available in the EMAN2.2 software package. ..
  5. Wagner J, Schaffer M, Fernández Busnadiego R. Cryo-electron tomography-the cell biology that came in from the cold. FEBS Lett. 2017;591:2520-2533 pubmed publisher
    ..We anticipate that these developments will soon make cryo-ET a fundamental technique in cell biology. ..
  6. Buono R, Leier A, Paez Valencia J, Pennington J, Goodman K, Miller N, et al. ESCRT-mediated vesicle concatenation in plant endosomes. J Cell Biol. 2017;216:2167-2177 pubmed publisher
    ..Consistent with a potential role as a diffusion barrier, we find that the ESCRT-III protein SNF7 remains associated with ILVs and is delivered to the vacuole for degradation. ..
  7. Patwardhan A. Trends in the Electron Microscopy Data Bank (EMDB). Acta Crystallogr D Struct Biol. 2017;73:503-508 pubmed publisher
    ..Overall, the numbers point to a rapid democratization of the field, with more countries and institutes becoming involved. ..
  8. Clare D, Siebert C, Hecksel C, Hagen C, Mordhorst V, Grange M, et al. Electron Bio-Imaging Centre (eBIC): the UK national research facility for biological electron microscopy. Acta Crystallogr D Struct Biol. 2017;73:488-495 pubmed publisher
    ..Finally, all raw data are archived, and a metadata catalogue and automated pipelines for data analysis are being developed. ..
  9. Mangala Prasad V, Klose T, Rossmann M. Assembly, maturation and three-dimensional helical structure of the teratogenic rubella virus. PLoS Pathog. 2017;13:e1006377 pubmed publisher
    ..These results present the first known example of a helical membrane-enveloped virus, while also providing a structural basis for its assembly and maturation pathway. ..

More Information

Publications18

  1. Biazik J, Vihinen H, Jokitalo E, Eskelinen E. Ultrastructural Characterization of Phagophores Using Electron Tomography on Cryoimmobilized and Freeze Substituted Samples. Methods Enzymol. 2017;587:331-349 pubmed publisher
    ..In this report, we describe our protocol for cryoimmobilization using high-pressure freezing and freeze substitution, and report our first findings on phagophore morphology using this approach. ..
  2. Cölfen H. Biomineralization: A crystal-clear view. Nat Mater. 2010;9:960-1 pubmed publisher
  3. Yao Q, Jewett A, Chang Y, Oikonomou C, Beeby M, Iancu C, et al. Short FtsZ filaments can drive asymmetric cell envelope constriction at the onset of bacterial cytokinesis. EMBO J. 2017;36:1577-1589 pubmed publisher
  4. Hu G, Liu J, Roux K, Taylor K. Structure of Simian Immunodeficiency Virus Envelope Spikes Bound with CD4 and Monoclonal Antibody 36D5. J Virol. 2017;91: pubmed publisher
    ..Our results show that the CD4-induced conformational dynamics of the spike enhances binding of the antibody. ..
  5. Oikonomou C, Jensen G. Cellular Electron Cryotomography: Toward Structural Biology In Situ. Annu Rev Biochem. 2017;86:873-896 pubmed publisher
    ..We also discuss the integration of ECT with other techniques, including lower-resolution fluorescence imaging and higher-resolution atomic structure determination, to cover the full scale of cellular processes. ..
  6. Nechipurenko I, Berciu C, Sengupta P, Nicastro D. Centriolar remodeling underlies basal body maturation during ciliogenesis in Caenorhabditis elegans. elife. 2017;6: pubmed publisher
    ..Our work provides a framework for future investigations into the mechanisms underlying BB remodeling. ..
  7. Serwas D, Su T, Roessler M, Wang S, Dammermann A. Centrioles initiate cilia assembly but are dispensable for maturation and maintenance in C. elegans. J Cell Biol. 2017;216:1659-1671 pubmed publisher
    ..Taken together, our results indicate that while centrioles are essential to initiate cilia formation, they are dispensable for cilia maturation and maintenance. ..
  8. Yang C, Svitkina T. Visualizing branched actin filaments in lamellipodia by electron tomography. Nat Cell Biol. 2011;13:1012-3; author reply 1013-4 pubmed publisher
  9. Wu S, Liu J, Reedy M, Tregear R, Winkler H, Franzini Armstrong C, et al. Electron tomography of cryofixed, isometrically contracting insect flight muscle reveals novel actin-myosin interactions. PLoS ONE. 2010;5: pubmed publisher
    ..The results support previous conclusions that the weak attachments preceding force generation are very different from strong binding attachments. ..