immunoblotting

Summary

Summary: Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.

Top Publications

  1. Rebelo S, Domingues S, Santos M, Fardilha M, Esteves S, Vieira S, et al. Identification of a novel complex A?PP:Fe65:PP1 that regulates A?PP Thr668 phosphorylation levels. J Alzheimers Dis. 2013;35:761-75 pubmed publisher
    ..Additionally, we show that this interaction correlates with A?PP Thr668 phosphorylation state, consistent with the role of protein (de)phosphorylation as a key mechanism in regulating cellular events...
  2. Liu H, Yao S, Dann S, Qin H, Elson C, Cong Y. ERK differentially regulates Th17- and Treg-cell development and contributes to the pathogenesis of colitis. Eur J Immunol. 2013;43:1716-26 pubmed publisher
  3. Janwan P, Intapan P, Yamasaki H, Laummaunwai P, Sawanyawisuth K, Wongkham C, et al. Application of recombinant Gnathostoma spinigerum matrix metalloproteinase-like protein for serodiagnosis of human gnathostomiasis by immunoblotting. Am J Trop Med Hyg. 2013;89:63-7 pubmed publisher
    ..The immunoblotting gave high sensitivity (100%) and specificity (94.7%). Positive and negative predictive values were 85...
  4. Emmerich C, Ordureau A, Strickson S, Arthur J, Pedrioli P, Komander D, et al. Activation of the canonical IKK complex by K63/M1-linked hybrid ubiquitin chains. Proc Natl Acad Sci U S A. 2013;110:15247-52 pubmed publisher
    ..Our study may help to resolve the debate about the relative importance of K63-pUb and M1-pUb chains in activating the canonical IKK complex. ..
  5. Liao J, Liu P, Hou G, Shao J, Yang J, Liu K, et al. Regulation of stem-like cancer cells by glutamine through β-catenin pathway mediated by redox signaling. Mol Cancer. 2017;16:51 pubmed publisher
    ..Molecular analyses including RNAi interfering, qRT-PCR, and immunoblotting were employed to investigate the molecular signaling in response to glutamine deprivation and its influence on ..
  6. Gao A, DU H, Gao Q, Wang Y, Wang X, Liu J, et al. Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's Disease. Am J Alzheimers Dis Other Demen. 2016;31:413-21 pubmed publisher
    ..b>Immunoblotting was used to observe the effect of TLJN on inflammatory mediator expression in an senescence-accelerated mouse ..
  7. Franco I, Pais S, Charro N, Mota L. Effector Translocation Assay: Differential Solubilization. Methods Mol Biol. 2017;1615:501-515 pubmed publisher
    ..Using appropriate controls, this fraction can be probed by immunoblotting for the presence of bacterial effector proteins delivered into host cells.
  8. Rossin A, Hueber A. Detection of S-Acylated CD95 by Acyl-Biotin Exchange. Methods Mol Biol. 2017;1557:189-198 pubmed publisher
    ..Resulting biotinylated proteins can be easily purified by an avidin capture and analyzed by SDS-PAGE and immunoblotting.
  9. Bahir W, Omar O, Rosales R, Hlusek M, Ziay G, Schauwers W, et al. Search for OIE-listed ruminant mycoplasma diseases in Afghanistan. BMC Vet Res. 2017;13:149 pubmed publisher
    ..However, no positive bands were seen when immunoblotting was carried out on all sera that gave suspect results...

More Information

Publications62

  1. Malkani N, Biggar K, Shehab M, Li S, Jansson T, Gupta M. Increased IGFBP-1 phosphorylation in response to leucine deprivation is mediated by CK2 and PKC. Mol Cell Endocrinol. 2016;425:48-60 pubmed publisher
    ..that leucine deprivation stimulated CK2 activity (enzymatic assay) and induced IGFBP-1 phosphorylation (immunoblotting/MRM-MS)...
  2. Pesonen M, Rysä J, Storvik M, Rilla K, Pasanen M, Vähäkangas K. Molecular targets of chloropicrin in human airway epithelial cells. Toxicol In Vitro. 2017;42:247-254 pubmed publisher
    ..Furthermore, confocal microscopy and immunoblotting indicated that cytoskeletal β-tubulin protein is a probable target of chloropicrin exposure...
  3. Kiene L, Homann S, Suvorava T, Rabausch B, Müller J, Kojda G, et al. Deletion of Hyaluronan Synthase 3 Inhibits Neointimal Hyperplasia in Mice. Arterioscler Thromb Vasc Biol. 2016;36:e9-16 pubmed publisher
    ..In addition, endothelial function as determined by acetylcholine-induced relaxation of aortic rings, immunoblotting of endothelial nitric oxide synthase, and arterial blood pressure were not affected...
  4. Lee M, Kim D, Yoon H, So D, Khalmuratova R, Rhee C, et al. Sirtuin 1 attenuates nasal polypogenesis by suppressing epithelial-to-mesenchymal transition. J Allergy Clin Immunol. 2016;137:87-98.e7 pubmed publisher
    ..Immunohistochemistry, immunoblotting, and immunoprecipitation were done to evaluate SIRT1, EMT, and hypoxic markers in human nasal epithelial cells ..
  5. Abozaid O, Moawed F, Farrag M, Abdel Aziz A. 4-(4-Hydroxy-3-methoxyphenyl)-2-butanone modulates redox signal in gamma-irradiation-induced nephrotoxicity in rats. Free Radic Res. 2017;:1-11 pubmed publisher
    ..Additionally, Western immunoblotting analysis of signalling growth factor protein; p38 MAPK was significantly overexpressed...
  6. Shen K, Hung J, Chang C, Weng Y, Wu M, Chen P. Solasodine inhibits invasion of human lung cancer cell through downregulation of miR-21 and MMPs expression. Chem Biol Interact. 2017;268:129-135 pubmed publisher
    ..b>Immunoblotting assays indicate that solasodine is effective in suppressing PI3K and Akt phosphorylation...
  7. Park G, Kim D, Kim Y, Kim S, Lee H, Yang J, et al. The Epstein-Barr virus causes epithelial-mesenchymal transition in human corneal epithelial cells via Syk/src and Akt/Erk signaling pathways. Invest Ophthalmol Vis Sci. 2014;55:1770-9 pubmed publisher
    ..This phenomenon may have implications for EBV-associated keratitis and molecular approaches to treatment. ..
  8. Chiang C, Uzoma I, Lane D, Memišević V, Alem F, Yao K, et al. A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. infection. Front Microbiol. 2015;6:683 pubmed publisher
    ..infections of which eight proteins were selected for further characterization by immunoblotting. Increased phosphorylation of AMPK-α1, Src, and GSK3β suggested the importance of their roles in ..
  9. Pinto A, da Rocha A, Oliveira L, Morais G, de Vicente L, Cintra D, et al. Levels of Hepatic Activating Transcription Factor 6 and Caspase-3 Are Downregulated in Mice after Excessive Training. Front Endocrinol (Lausanne). 2017;8:247 pubmed publisher
    ..After the grip force test, the livers and cardiac muscles (i.e., left ventricle) were removed and used for immunoblotting. All of the OT protocols led to similar responses in the performance parameters and displayed significantly ..
  10. Ottestad Hansen S, Hu Q, Follin Arbelet V, Bentea E, Sato H, Massie A, et al. The cystine-glutamate exchanger (xCT, Slc7a11) is expressed in significant concentrations in a subpopulation of astrocytes in the mouse brain. Glia. 2018;66:951-970 pubmed publisher
    ..The concentration of xCT protein in hippocampus was compared with that of the EAAT3 glutamate transporter by immunoblotting using a chimeric xCT-EAAT3 protein to normalize xCT and EAAT3 labeling intensities...
  11. Mutze K, Vierkotten S, Milosevic J, Eickelberg O, Königshoff M. Enolase 1 (ENO1) and protein disulfide-isomerase associated 3 (PDIA3) regulate Wnt/β-catenin-driven trans-differentiation of murine alveolar epithelial cells. Dis Model Mech. 2015;8:877-90 pubmed publisher
    ..This was accompanied by increased Wnt/β-catenin signaling, as analyzed by qRT-PCR and immunoblotting. Notably, ENO1 and PDIA3, along with T1α (podoplanin; an ATI cell marker), exhibited decreased protein ..
  12. Efentakis P, Andreadou I, Bibli S, Vasileiou S, Dagres N, Zoga A, et al. Ranolazine triggers pharmacological preconditioning and postconditioning in anesthetized rabbits through activation of RISK pathway. Eur J Pharmacol. 2016;789:431-438 pubmed publisher
    ..were subjected to the same interventions up to the 10th min of reperfusion where tissue samples were taken for immunoblotting of Akt, eNOS, ERK½ and GSK3? (RISK pathway)...
  13. Wang L, Luo H, Chen X, Jiang Y, Huang Q. Functional characterization of S100A8 and S100A9 in altering monolayer permeability of human umbilical endothelial cells. PLoS ONE. 2014;9:e90472 pubmed publisher
  14. Türk M, Schroder R, Khuller K, Hofmann A, Berwanger C, Ludolph A, et al. Genetic analysis of VCP and WASH complex genes in a German cohort of sporadic ALS-FTD patients. Neurobiol Aging. 2017;56:213.e1-213.e5 pubmed publisher
    ..Subsequent analyses included Sanger sequencing, in silico analyses, real-time PCR, and CCDC53 immunoblotting. We identified 1 patient with the heterozygous variant c.26C>T in CAPZA1, predicted to result in p...
  15. Dandekar M, Luse D, Hoffmann C, Cotton P, Peery T, Ruiz C, et al. Increased dopamine receptor expression and anti-depressant response following deep brain stimulation of the medial forebrain bundle. J Affect Disord. 2017;217:80-88 pubmed publisher
    ..MFB-DBS effects on the dopaminergic system were evaluated using immunoblotting for tyrosine hydroxylase (TH), dopamine transporter (DAT), and dopamine receptors (D1-D5), and high-..
  16. González M, de Mello W, Butler Browne G, Silva Barbosa S, Mouly V, Savino W, et al. HGF potentiates extracellular matrix-driven migration of human myoblasts: involvement of matrix metalloproteinases and MAPK/ERK pathway. Skelet Muscle. 2017;7:20 pubmed publisher
    ..We evaluated the expression of the HGF-receptor c-Met, laminin, and fibronectin receptors by immunoblotting, flow cytometry, or immunofluorescence and used Transwell assays to analyze myoblast migration on laminin 111 ..
  17. Lee J, Koh S, Guadagna S, Francis P, Esiri M, Chen C, et al. Altered relaxin family receptors RXFP1 and RXFP3 in the neocortex of depressed Alzheimer's disease patients. Psychopharmacology (Berl). 2016;233:591-8 pubmed publisher
    ..In this study, we characterized RXFP receptors for immunoblotting and measured RXFP1 and RXFP3 immunoreactivities in the postmortem neocortex of AD patients longitudinally ..
  18. Subramani R, GONZALEZ E, Nandy S, Arumugam A, Camacho F, Medel J, et al. Gedunin inhibits pancreatic cancer by altering sonic hedgehog signaling pathway. Oncotarget. 2017;8:10891-10904 pubmed publisher
    ..b>Immunoblotting was performed to analyze the effect of gedunin on the expression of key proteins involved in pancreatic cancer ..
  19. Gajate C, Mollinedo F. Isolation of Lipid Rafts Through Discontinuous Sucrose Gradient Centrifugation and Fas/CD95 Death Receptor Localization in Raft Fractions. Methods Mol Biol. 2017;1557:125-138 pubmed publisher
    ..4 °C, followed by sucrose gradient centrifugation, with subsequent analysis of Fas/CD95 death receptor localization in the raft fractions by immunoblotting. This method is also useful to localize additional proteins in membrane rafts.
  20. Tati R, Kristoffersson A, Manea Hedström M, Morgelin M, Wieslander J, van Kooten C, et al. Neutrophil Protease Cleavage of Von Willebrand Factor in Glomeruli - An Anti-thrombotic Mechanism in the Kidney. EBioMedicine. 2017;16:302-311 pubmed publisher
    ..VWF was incubated with human GBMs and VWF cleavage was assessed by multimer structure analysis, immunoblotting and mass spectrometry...
  21. Noël A, Barrier L, Ingrand S. The Tyr216 phosphorylated form of GSK3? contributes to tau phosphorylation at PHF-1 epitope in response to A? in the nucleus of SH-SY5Y cells. Life Sci. 2016;158:14-21 pubmed publisher
    ..The phophorylated forms (Ser9 and Tyr216) of GSK3? and pTau at PHF-1 epitope were measured by immunoblotting in nuclear extracts...
  22. Ungefroren H, Witte D, Fiedler C, Gädeken T, Kaufmann R, Lehnert H, et al. The Role of PAR2 in TGF-?1-Induced ERK Activation and Cell Motility. Int J Mol Sci. 2017;18: pubmed publisher
    ..was used to deplete cells of PAR2, followed by xCELLigence technology to measure cell migration, phospho-immunoblotting to assess ERK1/2 activation, and co-immunoprecipitation to detect a PAR2-ALK5 physical interaction...
  23. Myllynen P, Kurttila T, Vaskivuo L, Vahakangas K. DNA damage caused by benzo(a)pyrene in MCF-7 cells is increased by verapamil, probenecid and PSC833. Toxicol Lett. 2007;169:3-12 pubmed
    ..BPDE) was analyzed by synchronous fluorescence spectrophotometry and p53 protein by immunoblotting. BP metabolism was studied using thin layer chromatography (TLC)...
  24. Schell T, Kocher T, Wilm M, Seraphin B, Kulozik A, Hentze M. Complexes between the nonsense-mediated mRNA decay pathway factor human upf1 (up-frameshift protein 1) and essential nonsense-mediated mRNA decay factors in HeLa cells. Biochem J. 2003;373:775-83 pubmed
    ..Using affinity chromatography, Hupf1-2tag-associated proteins were isolated. MS and immunoblotting identified the NMD factors Hupf2 and Hupf3a/b as interaction partners of Hupf1...
  25. Harris E, Topf A, Vihola A, Evilä A, Barresi R, Hudson J, et al. A 'second truncation' in TTN causes early onset recessive muscular dystrophy. Neuromuscul Disord. 2017;27:1009-1017 pubmed publisher
    ..Cardiac dysfunction was present in two individuals. Muscle biopsy showed increased internal nuclei and immunoblotting identified reduction or absence of calpain-3 and demonstrated a marked reduction of C-terminal titin fragments...
  26. Sato F, Seino Sudo R, Okada M, Sakai H, Yumoto T, Wachi H. Lysyl Oxidase Enhances the Deposition of Tropoelastin through the Catalysis of Tropoelastin Molecules on the Cell Surface. Biol Pharm Bull. 2017;40:1646-1653 pubmed publisher
    ..b>Immunoblotting and immunofluorescence show that LOX and tropoelastin are present together with fibronectin on the cell ..
  27. He S, Park Y, Yorio T, Krishnamoorthy R. Endothelin-Mediated Changes in Gene Expression in Isolated Purified Rat Retinal Ganglion Cells. Invest Ophthalmol Vis Sci. 2015;56:6144-61 pubmed publisher
    ..Real-time PCR was used to validate gene expression, and immunocytochemistry and immunoblotting to confirm the protein expression of regulated genes...
  28. Emmerson P, Wang F, Du Y, Liu Q, Pickard R, Gonciarz M, et al. The metabolic effects of GDF15 are mediated by the orphan receptor GFRAL. Nat Med. 2017;23:1215-1219 pubmed publisher
    ..4,5,6). Together, our data demonstrate that GFRAL is a receptor for GDF15 that mediates the metabolic effects of GDF15. ..
  29. Villagra Blanco R, Angelova L, Conze T, Schares G, Bärwald A, Taubert A, et al. Seroprevalence of Neospora caninum-specific antibodies in German breeding bitches. Parasit Vectors. 2018;11:96 pubmed publisher
    ..33%) bitches using a commercial indirect enzyme-linked immunosorbent assay (ELISA). Immunoblotting analysis confirmed all seropositive samples detected by ELISA, proving that the animals were infected with N...
  30. Heimbach J, Reznikov L, Calkins C, Robinson T, Dinarello C, Harken A, et al. TNF receptor I is required for induction of macrophage heat shock protein 70. Am J Physiol Cell Physiol. 2001;281:C241-7 pubmed
    ..expression was induced by heat stress or exposure to endotoxin [lipopolysaccharide (LPS)] as determined by immunoblotting. HSP70 expression induced by either heat or LPS was markedly decreased in TNFR-I KO Mphi, whereas TNFR-II KO ..
  31. Chuzeville S, Dramsi S, Madec J, Haenni M, Payot S. Antigen I/II encoded by integrative and conjugative elements of Streptococcus agalactiae and role in biofilm formation. Microb Pathog. 2015;88:1-9 pubmed publisher
    ..The production and surface exposition of the antigen I/II encoded by this ICE was examined using RT-PCR and immunoblotting experiments...
  32. Shishodia G, Shukla S, Srivastava Y, Masaldan S, Mehta S, Bhambhani S, et al. Alterations in microRNAs miR-21 and let-7a correlate with aberrant STAT3 signaling and downstream effects during cervical carcinogenesis. Mol Cancer. 2015;14:116 pubmed publisher
    ..of miR-21 & let-7a and STAT3 & pSTAT3 (Y705) by PCR-based genotyping, quantitative real-time PCR and immunoblotting. Analysis of cancer tissues revealed an elevated miR-21 and reduced let-7a expression that correspond to the ..
  33. Mäkelä J, Mudò G, Pham D, Di Liberto V, Eriksson O, Louhivuori L, et al. Peroxisome proliferator-activated receptor-γ coactivator-1α mediates neuroprotection against excitotoxic brain injury in transgenic mice: role of mitochondria and X-linked inhibitor of apoptosis protein. Eur J Neurosci. 2016;43:626-39 pubmed publisher
    ..b>Immunoblotting showed that X-linked inhibitor of apoptosis protein (XIAP) was increased in PGC-1α transgenic hippocampus ..
  34. Sharma A, Sinha N, Siddiqui S, Mohan R. Role of 5'TG3'-interacting factors (TGIFs) in Vorinostat (HDAC inhibitor)-mediated Corneal Fibrosis Inhibition. Mol Vis. 2015;21:974-84 pubmed
    ..5 µM) to test its effect on TGIF mRNA and protein levels using qPCR and immunoblotting. Myofibroblast formation was induced with TGFβ1 (5 ng/ml) treatment under serum-free conditions...
  35. Li C, Yu T, Liu Y, Chen X, Zhang X. Topical Application of Insulin Accelerates Vessel Maturation of Wounds by Regulating Angiopoietin-1 in Diabetic Mice. Int J Low Extrem Wounds. 2015;14:353-64 pubmed publisher
    ..application neutralizing antibodies to these proteins; the specific protein was further confirmed using immunoblotting. On days 7 and 11, the blood vessel in insulin-treated wounds was surrounded by more α-smooth muscle actin (..
  36. Sami Saribas A, Cicalese S, Ahooyi T, Khalili K, Amini S, Sariyer I. HIV-1 Nef is released in extracellular vesicles derived from astrocytes: evidence for Nef-mediated neurotoxicity. Cell Death Dis. 2017;8:e2542 pubmed publisher
    ..that HIV-1 Nef-containing EVs were readily taken up by neurons as demonstrated by immunocytochemistry and immunoblotting. Furthermore, treatment of neurons with Nef-carrying EVs induced oxidative stress as evidenced by a decrease ..
  37. Yuri T, Yoshizawa K, Emoto Y, Kinoshita Y, Yuki M, Tsubura A. Effects of Dietary Xanthophylls, Canthaxanthin and Astaxanthin on N-Methyl-N-nitrosourea-induced Rat Mammary Carcinogenesis. In Vivo. 2016;30:795-800 pubmed
    ..42%; p<0.05), while the low and high canthaxanthin diets produced no significant inhibition. Adiponectin immunoblotting showed significantly higher expression in the 0...
  38. Zorzi V, Paciello F, Ziraldo G, Peres C, Mazzarda F, Nardin C, et al. Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function. Front Mol Neurosci. 2017;10:379 pubmed publisher
    ..Successful ablation of Panx1 was confirmed by RT-PCR and Western immunoblotting in the cochlea and brain of Panx1-/- mice...
  39. Mahmood J, Connors C, Alexander A, Pavlovic R, Samanta S, Soman S, et al. Cavernous Nerve Injury by Radiation Therapy May Potentiate Erectile Dysfunction in Rats. Int J Radiat Oncol Biol Phys. 2017;99:680-688 pubmed publisher
    ..the CN using quantitative polymerase chain reaction, conduction studies, transmission electron microscopy, and immunoblotting. Masson trichrome staining was performed to elucidate fibrosis level in penile tissues...
  40. Petrovski S, Parrott R, Roberts J, Huang H, Yang J, Gorentla B, et al. Dominant Splice Site Mutations in PIK3R1 Cause Hyper IgM Syndrome, Lymphadenopathy and Short Stature. J Clin Immunol. 2016;36:462-71 pubmed publisher
    ..They were investigated with in vitro immune studies, RT-PCR, and immunoblotting studies of the mutation's effect on mTOR pathway signaling...
  41. Shang V, O Sullivan S, Kendall D, Roberts R. The endogenous cannabinoid anandamide increases human airway epithelial cell permeability through an arachidonic acid metabolite. Pharmacol Res. 2016;105:152-63 pubmed publisher
    ..and expression of the tight junction proteins, occludin and ZO-1, were determined using Western immunoblotting. Anandamide produced a significant reduction in TEER, which was unaffected by cannabinoid receptor antagonists,..
  42. Del Vecchio F, Gallo F, Di Marco A, Mastroiaco V, Caianiello P, Zazzeroni F, et al. Bioinformatics approach to predict target genes for dysregulated microRNAs in hepatocellular carcinoma: study on a chemically-induced HCC mouse model. BMC Bioinformatics. 2015;16:408 pubmed publisher
    ..The expression of one (ANK3) among the 15 predicted targets was further validated by immunoblotting. Then, enrichment annotation analysis was performed revealing significant clusters, including some playing a ..
  43. Inoue S, Moriya T, Morita R, Kuwata K, Thul S, Sarangi B, et al. Characterization of UDP-glucosyltransferase from Indigofera tinctoria. Plant Physiol Biochem. 2017;121:226-233 pubmed publisher
    ..Expression levels of itUGT mRNA and protein in plant tissues were examined by UGT assay, immunoblotting, and semi-quantitative reverse transcription polymerase chain reaction...
  44. Ju Z, Ma J, Wang C, Yu J, Qiao Y, Hei F. Exosomes from iPSCs Delivering siRNA Attenuate Intracellular Adhesion Molecule-1 Expression and Neutrophils Adhesion in Pulmonary Microvascular Endothelial Cells. Inflammation. 2017;40:486-496 pubmed publisher
    ..b>Immunoblotting confirmed the presence of the typical exosomal markers, including CD63, TSG 101, and Alix...
  45. Balda M, Anderson J. Two classes of tight junctions are revealed by ZO-1 isoforms. Am J Physiol. 1993;264:C918-24 pubmed
    ..Both immunoblotting and ribonuclease protection analysis confirmed this pattern of expression...
  46. Liu T, Wu X, Chen T, Luo Z, Hu X. Downregulation of DNMT3A by miR-708-5p Inhibits Lung Cancer Stem Cell-like Phenotypes through Repressing Wnt/β-catenin Signaling. Clin Cancer Res. 2018;24:1748-1760 pubmed publisher
    ..b>Immunoblotting, dual luciferase reporter, and immunocytochemistry were performed to determine the target of miR-708-5p...
  47. Naito N, Mizuno T, Nishimura T, Kishimoto S, Takewa Y, Eura Y, et al. Influence of a Rotational Speed Modulation System Used With an Implantable Continuous-Flow Left Ventricular Assist Device on von Willebrand Factor Dynamics. Artif Organs. 2016;40:877-83 pubmed publisher
    ..Levels of vWF:Ag, vWF:Rco, and Rco/Ag did not change significantly during the test. Immunoblotting analysis of vWF multimers showed slight degradation of HMWM in all groups, but there were no significant ..
  48. Yao W, Li X, Zhao B, Du G, Feng P, Chen W. Combined effect of TNF-? and cyclic stretching on gene and protein expression associated with mineral metabolism in cementoblasts. Arch Oral Biol. 2017;73:88-93 pubmed publisher
    ..and protein expression CAP, Col I, BSP, OPG, RANKL and RUNX2 were detected using RT- PCR and ELISA/Western immunoblotting methods, respectively...
  49. Falsafi S, Ghafari M, Miklósi A, Engidawork E, Gröger M, Höger H, et al. Mouse hippocampal GABAB1 but not GABAB2 subunit-containing receptor complex levels are paralleling retrieval in the multiple-T-maze. Front Behav Neurosci. 2015;9:276 pubmed publisher
    ..Hippocampi were taken, membrane proteins extracted and run on blue native PAGE followed by immunoblotting with specific antibodies against GABAB1, GABAB1a, and GABAB2...
  50. Hsieh C, Chen Y, Lin C, Tang K, Chen H, Hung W, et al. Elevated Expression of the NLRP3 Inflammasome and Its Correlation with Disease Activity in Adult-onset Still Disease. J Rheumatol. 2017;44:1142-1150 pubmed publisher
    ..treated with the potential activator [imiquimod (IMQ)] or inhibitor of NLRP3 were evaluated using qPCR and immunoblotting, respectively. The supernatant levels of interleukin (IL)-1β and IL-18 were determined by ELISA...
  51. Zhou W, Xu J, Li H, Xu M, Chen Z, Wei W, et al. Neddylation E2 UBE2F Promotes the Survival of Lung Cancer Cells by Activating CRL5 to Degrade NOXA via the K11 Linkage. Clin Cancer Res. 2017;23:1104-1116 pubmed publisher
    ..b>Experimental Design: Methods of immunohistochemistry and immunoblotting were utilized to examine UBE2F protein expression...
  52. Oikawa D, Shiota M, Tokunaga F, Wanibuchi H. Generation of Rat Monoclonal Antibodies Specific for DZIP3. Monoclon Antib Immunodiagn Immunother. 2018;37:153-157 pubmed publisher
    ..In immunoblotting analyses, our antibodies detected endogenous human and mouse DZIP3...
  53. Yadav M, Singh A, Kumar H, Rao G, Chakravarti B, Gurjar A, et al. Epidermal growth factor receptor inhibitor cancer drug gefitinib modulates cell growth and differentiation of acute myeloid leukemia cells via histamine receptors. Biochim Biophys Acta. 2016;1860:2178-90 pubmed publisher
    ..Signaling events were analyzed by homogenous time-resolved fluorescence and immunoblotting. Cellular proliferation and differentiation were assessed by ATP measurement, trypan blue exclusion, 5-bromo-2'..