multilocus sequence typing


Summary: Direct nucleotide sequencing of gene fragments from multiple housekeeping genes for the purpose of phylogenetic analysis, organism identification, and typing of species, strain, serovar, or other distinguishable phylogenetic level.

Top Publications

  1. Balassiano I, Vital Brazil J, Ramos T, Timm L, Pereira M. Molecular and serological characterization of Leptospira kirschneri serogroup Pomona isolated from a human case in a Brazilian rural area. Rev Soc Bras Med Trop. 2017;50:396-398 pubmed publisher
    ..kirschneri serogroup Pomona serovar Mozdok. This study contributes to the local epidemiological knowledge of leptospirosis, prevention of the disease by vaccines, and improvements in its diagnosis. ..
  2. Day J, Qihui S, Thanh L, Trieu P, Van A, Thu N, et al. Comparative genomics of Cryptococcus neoformans var. grubii associated with meningitis in HIV infected and uninfected patients in Vietnam. PLoS Negl Trop Dis. 2017;11:e0005628 pubmed publisher
    ..grubii. ..
  3. Kaczmarek M, Mullett M, McDonald J, Denman S. Multilocus sequence typing provides insights into the population structure and evolutionary potential of Brenneria goodwinii, associated with acute oak decline. PLoS ONE. 2017;12:e0178390 pubmed publisher
    ..The newly emerged STs subsequently undergo clonal expansion to become dominant genotypes within their specific geographical locations and even within the individual host oak trees. ..
  4. van der Linden M, Otten J, Bergmann C, Latorre C, Liñares J, Hakenbeck R. Insight into the Diversity of Penicillin-Binding Protein 2x Alleles and Mutations in Viridans Streptococci. Antimicrob Agents Chemother. 2017;61: pubmed publisher
    ..b>Multilocus sequence typing (MLST) confirmed 35 isolates as the species S...
  5. Naas T, Cuzon G, Gaillot O, Courcol R, Nordmann P. When carbapenem-hydrolyzing beta-lactamase Kpc meets Escherichia coli ST131 in France. Antimicrob Agents Chemother. 2011;55:4933-4 pubmed publisher
  6. Helene L, Delamuta J, Ribeiro R, Hungria M. Bradyrhizobium mercantei sp. nov., a nitrogen-fixing symbiont isolated from nodules of Deguelia costata (syn. Lonchocarpus costatus). Int J Syst Evol Microbiol. 2017;67:1827-1834 pubmed publisher
    ..nov.; SEMIA 6399T (=CNPSo 1165T=BR 6010T=U675T=LMG 30031T) was chosen as the type strain. ..
  7. Zhu W, Zhang H, Xiang X, Zhong L, Yang L, Guo J, et al. Reassortant Eurasian Avian-Like Influenza A(H1N1) Virus from a Severely Ill Child, Hunan Province, China, 2015. Emerg Infect Dis. 2016;22:1930-1936 pubmed publisher
    ..Human-to-human transmission of influenza A/Hunan/42443/2015(H1N1) virus has not been detected; however, given the circulation of novel EA-H1N1 viruses in pigs, enhanced surveillance should be instituted among swine and humans. ..
  8. Zhang J, John Kelly P, Lu G, Cruz Martinez L, Wang C. Rickettsia in mosquitoes, Yangzhou, China. Emerg Microbes Infect. 2016;5:e108 pubmed publisher
  9. Feasey N, Cain A, Msefula C, Pickard D, Alaerts M, Aslett M, et al. Drug resistance in Salmonella enterica ser. Typhimurium bloodstream infection, Malawi. Emerg Infect Dis. 2014;20:1957-9 pubmed publisher

More Information


  1. Jelocnik M, Branley J, Heller J, Raidal S, Alderson S, Galea F, et al. Multilocus sequence typing identifies an avian-like Chlamydia psittaci strain involved in equine placentitis and associated with subsequent human psittacosis. Emerg Microbes Infect. 2017;6:e7 pubmed publisher
  2. Idris H, Labeda D, Nouioui I, Castro J, Del Carmen Montero Calasanz M, Bull A, et al. Streptomyces aridus sp. nov., isolated from a high altitude Atacama Desert soil and emended description of Streptomyces noboritoensis Isono et al. 1957. Antonie Van Leeuwenhoek. 2017;110:705-717 pubmed publisher
    ..melanogenes and S. noboritoensis type strains belong to a single species, it is proposed that S. melanogenes be recognised as a heterotypic synonym of S. noboritoensis for which an emended description is given. ..
  3. Nijhuis R, Veldman K, Schelfaut J, van Essen Zandbergen A, Wessels E, Claas E, et al. Detection of the plasmid-mediated colistin-resistance gene mcr-1 in clinical isolates and stool specimens obtained from hospitalized patients using a newly developed real-time PCR assay. J Antimicrob Chemother. 2016;71:2344-6 pubmed publisher
  4. Covey P, Kuwitzky B, Hanson M, Webb K. Multilocus analysis using putative fungal effectors to describe a population of Fusarium oxysporum from sugar beet. Phytopathology. 2014;104:886-96 pubmed publisher
    ..Of these, Fmk1, Fow1, PelA, Rho1, Sge1, Snf1, and Ste12 were significant in relating clade designations and PelD, and Prt1 were significant for correlating with pathogenicity in F. oxysporum f. sp. betae. ..
  5. Chen S, Zhang H, Liu X, Wang W, Hou S, Li T, et al. Increasing threat of brucellosis to low-risk persons in urban settings, China. Emerg Infect Dis. 2014;20:126-30 pubmed publisher
    ..This finding implies that the increasing threat of brucellosis might affect low-risk persons in urban settings in China. ..
  6. Georgi E, Walter M, Pfalzgraf M, Northoff B, Holdt L, Scholz H, et al. Whole genome sequencing of Brucella melitensis isolated from 57 patients in Germany reveals high diversity in strains from Middle East. PLoS ONE. 2017;12:e0175425 pubmed publisher
    ..This is the first report of a detailed genetic investigation of an extensive collection of B. melitensis strains isolated from human cases in Germany. ..
  7. Msaddak A, Rejili M, Durán D, Rey L, Imperial J, Palacios J, et al. Members of Microvirga and Bradyrhizobium genera are native endosymbiotic bacteria nodulating Lupinus luteus in Northern Tunisian soils. FEMS Microbiol Ecol. 2017;93: pubmed publisher
    ..canariense/B. lupini lineages, and the remaining strains belong to two groups not previously identified as L. luteus endosymbionts: one corresponding to a new clade of Bradyrhizobium and the other to the genus Microvirga...
  8. Varela A, Macedo G, Nunes O, Manaia C. Genetic characterization of fluoroquinolone resistant Escherichia coli from urban streams and municipal and hospital effluents. FEMS Microbiol Ecol. 2015;91: pubmed publisher
    ..streams, wastewater treatment plants and hospital effluent between 2004 and 2012, were compared based on multilocus sequence typing (MLST), quinolone and beta-lactam resistance determinants and plasmid replicon type...
  9. Yang X, Qian S, Yao K, Wang L, Liu Y, Dong F, et al. Multiresistant ST59-SCCmec IV-t437 clone with strong biofilm-forming capacity was identified predominantly in MRSA isolated from Chinese children. BMC Infect Dis. 2017;17:733 pubmed publisher
    ..Isolates were typed by multilocus sequence typing (MLST), spa and SCCmec typing (for Methicillin-resistant SA [MRSA] only)...
  10. Nielsen J, Hansen F, Littauer P, Schønning K, Hammerum A. An NDM-1-producing Escherichia coli obtained in Denmark has a genetic profile similar to an NDM-1-producing E. coli isolate from the UK. J Antimicrob Chemother. 2012;67:2049-51 pubmed publisher
  11. Umeda K, Nakamura H, Yamamoto K, Nishina N, Yasufuku K, Hirai Y, et al. Molecular and epidemiological characterization of staphylococcal foodborne outbreak of Staphylococcus aureus harboring seg, sei, sem, sen, seo, and selu genes without production of classical enterotoxins. Int J Food Microbiol. 2017;256:30-35 pubmed publisher
    ..These findings suggest new SE/SEls as a potential cause of foodborne outbreaks. ..
  12. Inns T, Lane C, Peters T, Dallman T, Chatt C, McFarland N, et al. A multi-country Salmonella Enteritidis phage type 14b outbreak associated with eggs from a German producer: 'near real-time' application of whole genome sequencing and food chain investigations, United Kingdom, May to September 2014. Euro Surveill. 2015;20: pubmed
    ..Combined with food traceback information, this confirmed that the UK outbreak was also linked to a German producer. ..
  13. Zhang C, Zhang J, Zhao J, Zhao X, Zhao D, Yin H, et al. Serratia oryzae sp. nov., isolated from rice stems. Int J Syst Evol Microbiol. 2017;67:2928-2933 pubmed publisher
    ..Characterization by genotypic and phenotypic analysis indicated that J11-6T (=ACCC 19934T=KCTC 52529T) represents a novel species of the genus Serratia, for which the name Serratia oryzae sp. nov. is proposed. ..
  14. Limmathurotsakul D, Wongsuvan G, Aanensen D, Ngamwilai S, Saiprom N, Rongkard P, et al. Melioidosis caused by Burkholderia pseudomallei in drinking water, Thailand, 2012. Emerg Infect Dis. 2014;20:265-8 pubmed publisher
    ..The multilocus sequence type of B. pseudomallei from clinical specimens and water samples were identical for 2 patients. This finding suggests that drinking water is a preventable source of B. pseudomallei infection. ..
  15. Yan D, Chen Y, Lv T, Huang Y, Yang J, Li Y, et al. Clostridium difficile colonization and infection in patients with hepatic cirrhosis. J Med Microbiol. 2017;66:1483-1488 pubmed publisher
    ..difficile were isolated from patients with hepatic cirrhosis within 48?h after admission, followed by multilocus sequence typing (MLST)...
  16. Tohno M, Tanizawa Y, Irisawa T, Masuda T, Sakamoto M, Arita M, et al. Lactobacillus silagincola sp. nov. and Lactobacillus pentosiphilus sp. nov., isolated from silage. Int J Syst Evol Microbiol. 2017;67:3639-3644 pubmed publisher
    ..nov. and Lactobacillus pentosiphilus sp. nov., respectively. ..
  17. Zheng M, Piao C, Xue H, Guo M, Li Y. Brenneria populi subsp. brevivirga subsp. nov. isolated from symptomatic bark of Populus × euramericana canker, and description of Brenneria populi subsp. populi subsp. nov. Int J Syst Evol Microbiol. 2017;67:3633-3638 pubmed publisher
    ..populi subsp. brevivirga subsp. nov. is proposed with D8-10-4-5T (=CFCC 11935T=KCTC 42841T) as the type strain, with the automatic creation of B. populi subsp. populi subsp. nov. (type strain D9-5T=CFCC 11963T=KCTC 42088T). ..
  18. Tilburg J, Roest H, Buffet S, Nabuurs Franssen M, Horrevorts A, Raoult D, et al. Epidemic genotype of Coxiella burnetii among goats, sheep, and humans in the Netherlands. Emerg Infect Dis. 2012;18:887-9 pubmed publisher
  19. Tada T, Tsuchiya M, Shimada K, Nga T, Thu L, Phu T, et al. Dissemination of Carbapenem-resistant Klebsiella pneumoniae clinical isolates with various combinations of Carbapenemases (KPC-2, NDM-1, NDM-4, and OXA-48) and 16S rRNA Methylases (RmtB and RmtC) in Vietnam. BMC Infect Dis. 2017;17:467 pubmed publisher
    ..Antimicrobial susceptibility tests and whole genome sequencing were performed. Multilocus sequence typing and the presence of drug resistant genes were determined and a maximum-likelihood phylogenetic tree was ..
  20. Jiao Y, Qin Y, Liu J, Li Q, Dong Y, Shang Y, et al. Risk factors for carbapenem-resistant Klebsiella pneumoniae infection/colonization and predictors of mortality: a retrospective study. Pathog Glob Health. 2015;109:68-74 pubmed publisher
    ..pneumoniae (CSKP) infection/colonization at the same site. Homology analysis was conducted by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Potential resistance genes were detected by PCR...
  21. Cunha M, Saidenberg A, Moreno A, Ferreira A, Vieira M, Gomes T, et al. Pandemic extra-intestinal pathogenic Escherichia coli (ExPEC) clonal group O6-B2-ST73 as a cause of avian colibacillosis in Brazil. PLoS ONE. 2017;12:e0178970 pubmed publisher
    ..These results further suggest the zoonotic potential of some APEC clonal lineages and their possible role in the epidemiology of human ExPEC, in addition to providing the first description of the O6-B2-ST73 clonal group in poultry. ..
  22. Boonkhot P, Tadee P, Yamsakul P, Pocharoen C, Chokesajjawatee N, Patchanee P. Class 1 integrons characterization and multilocus sequence typing of Salmonella spp. from swine production chains in Chiang Mai and Lamphun provinces, Thailand. Jpn J Vet Res. 2015;63:83-94 pubmed
    ..organisms obtained from swine production chains in Chiang Mai and Lamphun provinces, Thailand, using multilocus sequence typing (MLST) and comparison of genetic diversity of sequence types of Salmonella from this study with ..
  23. Tettelin H, Davidson R, Agrawal S, Aitken M, Shallom S, Hasan N, et al. High-level relatedness among Mycobacterium abscessus subsp. massiliense strains from widely separated outbreaks. Emerg Infect Dis. 2014;20:364-71 pubmed publisher
    ..We propose 2 diagnostic strategies that use partial sequencing of rpoB and secA1 genes and a multilocus sequence typing protocol.
  24. Lester C, Olsen S, Jakobsen L, Arpi M, Fuursted K, Hansen D, et al. Emergence of extended-spectrum ?-lactamase (ESBL)-producing Klebsiella pneumoniae in Danish hospitals; this is in part explained by spread of two CTX-M-15 clones with multilocus sequence types 15 and 16 in Zealand. Int J Antimicrob Agents. 2011;38:180-2 pubmed publisher
  25. Margos G, Fedorova N, Kleinjan J, Hartberger C, Schwan T, Sing A, et al. Borrelia lanei sp. nov. extends the diversity of Borrelia species in California. Int J Syst Evol Microbiol. 2017;67:3872-3876 pubmed publisher
    ..The type strain for Borrelia lanei sp. nov., strain CA28-91T, has been deposited to two culture collections (=DSM 17992T=CIP 109135T). ..
  26. de Been M, Pinholt M, Top J, Bletz S, Mellmann A, van Schaik W, et al. Core Genome Multilocus Sequence Typing Scheme for High- Resolution Typing of Enterococcus faecium. J Clin Microbiol. 2015;53:3788-97 pubmed
    ..Since the start of the 21st century, multilocus sequence typing (MLST) has been used to study the molecular epidemiology of E. faecium...
  27. Sugawara Y, Akeda Y, Sakamoto N, Takeuchi D, Motooka D, Nakamura S, et al. Genetic characterization of blaNDM-harboring plasmids in carbapenem-resistant Escherichia coli from Myanmar. PLoS ONE. 2017;12:e0184720 pubmed publisher
    ..The results indicate circulation of phylogenetically distinct strains of carbapenem-resistant E. coli with various plasmids harboring blaNDM genes in the hospital...
  28. Teixeira M, Theodoro R, Niño Vega G, Bagagli E, Felipe M. Paracoccidioides species complex: ecology, phylogeny, sexual reproduction, and virulence. PLoS Pathog. 2014;10:e1004397 pubmed publisher
  29. Suttisunhakul V, Pumpuang A, Ekchariyawat P, Wuthiekanun V, Elrod M, Turner P, et al. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of Burkholderia pseudomallei from Asia and Australia and differentiation between Burkholderia species. PLoS ONE. 2017;12:e0175294 pubmed publisher
    ..pseudomallei and B. mallei, while the recA tree did not. MALDI-TOF MS is an accurate method for the identification of B. pseudomallei, and discriminates between this and other related Burkholderia species. ..
  30. Olaitan A, Thongmalayvong B, Akkhavong K, Somphavong S, Paboriboune P, Khounsy S, et al. Clonal transmission of a colistin-resistant Escherichia coli from a domesticated pig to a human in Laos. J Antimicrob Chemother. 2015;70:3402-4 pubmed publisher
  31. Mediavilla J, Chen L, Uhlemann A, Hanson B, Rosenthal M, Stanak K, et al. Methicillin-susceptible Staphylococcus aureus ST398, New York and New Jersey, USA. Emerg Infect Dis. 2012;18:700-2 pubmed publisher
  32. Mustafa A, Habibi N, Osman A, Shaheed F, Khan M. Species identification and molecular typing of human Brucella isolates from Kuwait. PLoS ONE. 2017;12:e0182111 pubmed publisher
    ..Furthermore, the combined minimum spanning tree analysis demonstrated that, compared to MLVA types discovered all over the world, the Kuwaiti isolates were a distinct group of MLVA-11 and MLVA-16 types in the East Mediterranean Region. ..
  33. Potron A, Schrenzel J, Poirel L, Renzi G, Cherkaoui A, Nordmann P. Emergence of OXA-48-producing Enterobacteriaceae in Switzerland. Int J Antimicrob Agents. 2012;40:563-4 pubmed publisher
  34. Li N, Xiao L, Cama V, Ortega Y, Gilman R, Guo M, et al. Genetic recombination and Cryptosporidium hominis virulent subtype IbA10G2. Emerg Infect Dis. 2013;19:1573-82 pubmed publisher
    ..These findings improve our understanding of the origin and evolution of C. hominis subtypes and the spread of virulent subtypes. ..
  35. Lázaro Perona F, Sarria Visa A, Ruiz Carrascoso G, Mingorance J, Garcia Rodriguez J, Gómez Gil R. Klebsiella pneumoniae co-producing NDM-7 and OXA-48 carbapenemases isolated from a patient with prolonged hospitalisation. Int J Antimicrob Agents. 2017;49:112-113 pubmed publisher
  36. Amato E, Filipello V, Gori M, Lomonaco S, Losio M, Parisi A, et al. Identification of a major Listeria monocytogenes outbreak clone linked to soft cheese in Northern Italy - 2009-2011. BMC Infect Dis. 2017;17:342 pubmed publisher
    ..cluster grouped 31 isolates that belonged to serotype 1/2a and Sequence Type 38 (ST38) as defined by Multilocus Sequence Typing (MLST)...
  37. Kenna D, Lilley D, Coward A, Martin K, Perry C, Pike R, et al. Prevalence of Burkholderia species, including members of Burkholderia cepacia complex, among UK cystic and non-cystic fibrosis patients. J Med Microbiol. 2017;66:490-501 pubmed publisher
    ..81). The dominance of B. multivorans in CF, the presence of a B. cepacia cluster among non-CF patients and the existence of putative novel species all highlighted the continuing role of Burkholderia species as opportunistic pathogens. ..
  38. Song J, Cui B. Phylogeny, divergence time and historical biogeography of Laetiporus (Basidiomycota, Polyporales). BMC Evol Biol. 2017;17:102 pubmed publisher
    ..Four intercontinental dispersal routes and a possible concealed dispersal route were established for the first time. ..
  39. Vicente J, Rothwell S, Holub E, Studholme D. Pathogenic, phenotypic and molecular characterisation of Xanthomonas nasturtii sp. nov. and Xanthomonas floridensis sp. nov., new species of Xanthomonas associated with watercress production in Florida. Int J Syst Evol Microbiol. 2017;67:3645-3654 pubmed publisher
    ..Although the importance of the new pathogenic species is yet to be determined, the bacterial disease that it causes constitutes a threat to watercress production and its distribution should be monitored...
  40. Potron A, Bernabeu S, Cuzon G, Ponties V, Blanchard H, Seringe E, et al. Analysis of OXA-204 carbapenemase-producing Enterobacteriaceae reveals possible endoscopy-associated transmission, France, 2012 to 2014. Euro Surveill. 2017;22: pubmed publisher
    ..The blaOXA-204 gene was located on a 150-kb IncA/C plasmid, isolated from various enterobacterial species in the same patient, indicating a high conjugative ability of this genetic vehicle...
  41. Zhang X, Doi Y, Huang X, Li H, Zhong L, Zeng K, et al. Possible Transmission of mcr-1-Harboring Escherichia coli between Companion Animals and Human. Emerg Infect Dis. 2016;22:1679-81 pubmed publisher
  42. Lynagh Y, Mac Aogáin M, Walsh A, Rogers T, Unemo M, Crowley B. Detailed characterization of the first high-level azithromycin-resistant Neisseria gonorrhoeae cases in Ireland. J Antimicrob Chemother. 2015;70:2411-3 pubmed publisher
  43. Mezzatesta M, Gona F, Caio C, Adembri C, Dell utri P, Santagati M, et al. Emergence of an extensively drug-resistant ArmA- and KPC-2-producing ST101 Klebsiella pneumoniae clone in Italy. J Antimicrob Chemother. 2013;68:1932-4 pubmed publisher
  44. Feodoroff B, de Haan C, Ellström P, Sarna S, Hänninen M, Rautelin H. Clonal distribution and virulence of Campylobacter jejuni isolates in blood. Emerg Infect Dis. 2013;19:1653-5 pubmed publisher
    ..Seventy-three C. jejuni isolates from blood collected in Finland were analyzed by multilocus sequence typing and serum resistance...
  45. Moura A, Kretz C, Ferreira Í, Nunes A, de Moraes J, Reis M, et al. Molecular characterization of Neisseria meningitidis isolates recovered from 11-19-year-old meningococcal carriers in Salvador, Brazil. PLoS ONE. 2017;12:e0185038 pubmed publisher
    ..isolates, collected from adolescents resident in Salvador, Brazil during 2014, were characterized by multilocus sequence typing, genotyping or whole-genome sequencing. Most were nongroupable (61.0%), followed by genogroups B (11...
  46. Beukers A, Zaheer R, Goji N, Amoako K, Chaves A, Ward M, et al. Comparative genomics of Enterococcus spp. isolated from bovine feces. BMC Microbiol. 2017;17:52 pubmed publisher
    ..faecium from the NCBI database demonstrated differential clustering of commensal and clinical isolates, suggesting that these strains may be specifically adapted to their respective environments. ..
  47. Sulaiman I, Jacobs E, Simpson S, Kerdahi K. Multilocus Genetic Characterization of Lactobacillus fermentum Isolated from Ready-to-Eat Canned Food. J Food Prot. 2017;80:963-968 pubmed publisher
    ..The recovered isolates were subsequently sequenced at rRNA and gyrB loci. Afterward, multilocus sequence typing (MLST) was performed characterizing 11 additional known MLST loci (clpX, dnaA, dnaK, groEL, murC, murE, ..
  48. Odetoyin B, Labar A, Lamikanra A, Aboderin A, Okeke I. Classes 1 and 2 integrons in faecal Escherichia coli strains isolated from mother-child pairs in Nigeria. PLoS ONE. 2017;12:e0183383 pubmed publisher
    ..There is a need for improved surveillance for resistance and its mechanisms of dissemination and persistence and mobility of resistance genes in the community and clinical settings. ..
  49. Poirel L, Barbosa Vasconcelos A, Simoes R, da Costa P, Liu W, Nordmann P. Environmental KPC-producing Escherichia coli isolates in Portugal. Antimicrob Agents Chemother. 2012;56:1662-3 pubmed publisher
  50. Chalmers R, Caccio S. Towards a consensus on genotyping schemes for surveillance and outbreak investigations of Cryptosporidium, Berlin, June 2016. Euro Surveill. 2016;21: pubmed publisher
  51. Mohsin M, Raza S, Roschanski N, Schaufler K, Guenther S. First description of plasmid-mediated colistin-resistant extended-spectrum ?-lactamase-producing Escherichia coli in a wild migratory bird from Asia. Int J Antimicrob Agents. 2016;48:463-4 pubmed publisher
  52. Alanio A, Desnos Ollivier M, Garcia Hermoso D, Bretagne S. Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?. Clin Microbiol Rev. 2017;30:671-707 pubmed publisher
    ..Two methods have emerged because of their high discriminatory power and reproducibility: multilocus sequence typing (MLST) and microsatellite length polymorphism (MLP) using short tandem repeat (STR) markers...
  53. Hopkins K, Meunier D, Findlay J, Mustafa N, Parsons H, Pike R, et al. SPM-1 metallo-?-lactamase-producing Pseudomonas aeruginosa ST277 in the UK. J Med Microbiol. 2016;65:696-7 pubmed publisher
  54. Bialek Davenet S, Criscuolo A, Ailloud F, Passet V, Jones L, Delannoy Vieillard A, et al. Genomic definition of hypervirulent and multidrug-resistant Klebsiella pneumoniae clonal groups. Emerg Infect Dis. 2014;20:1812-20 pubmed publisher
    ..A total of 694 highly conserved genes were included in a core-genome multilocus sequence typing scheme, and cluster analysis of the data enabled precise definition of globally distributed ..
  55. Trembizki E, Buckley C, Lawrence A, Lahra M, Whiley D. Characterization of a novel Neisseria gonorrhoeae penicillinase-producing plasmid isolated in Australia in 2012. Antimicrob Agents Chemother. 2014;58:4984-5 pubmed publisher
  56. Opazo A, Vali L, Al Obaid K, Dashti A, Amyes S. Novel genetic structure harbouring blaPER-1 in ceftazidime-resistant Acinetobacter baumannii isolated from Kuwait. Int J Antimicrob Agents. 2014;43:383-4 pubmed publisher
  57. Kapatai G, Sheppard C, Troxler L, Litt D, Furrer J, Hilty M, et al. Pneumococcal 23B Molecular Subtype Identified Using Whole Genome Sequencing. Genome Biol Evol. 2017;9:2122-2135 pubmed publisher
    ..Therefore, although the 23B1 variant appears to have no phenotypic impact and cannot be considered as novel serotype, it appears to have led to a genetic restructuring of the UK serotype 23B population...
  58. Park I, Kim K, Andrade A, Briles D, McDaniel L, Nahm M. Nontypeable pneumococci can be divided into multiple cps types, including one type expressing the novel gene pspK. MBio. 2012;3: pubmed publisher
    ..e., bacteria satisfying the conventional definition but without the multilocus sequence typing [MLST]-based definition of S. pneumoniae) by the traditional criteria...
  59. Guernier V, Richard V, Nhan T, Rouault E, Tessier A, Musso D. Leptospira diversity in animals and humans in Tahiti, French Polynesia. PLoS Negl Trop Dis. 2017;11:e0005676 pubmed publisher
    ..Thus, further investigations will be required to determine their significance in leptospirosis transmission in FP. ..
  60. Gawryszewska I, Żabicka D, Hryniewicz W, Sadowy E. Linezolid-resistant enterococci in Polish hospitals: species, clonality and determinants of linezolid resistance. Eur J Clin Microbiol Infect Dis. 2017;36:1279-1286 pubmed publisher
    ..Transferability and apparent lack of a biological cost of resistance suggest that E. faecalis may be a natural reservoir of optrA, an emerging mechanism of oxazolidinone resistance. ..
  61. Ali H, Salem M, El Shikh M, Megeed A, Alogaibi Y, Talea I. Investigation of the Virulence Factors and Molecular Characterization of the Clonal Relations of Multidrug-Resistant Acinetobacter baumannii Isolates. J AOAC Int. 2017;100:152-158 pubmed publisher
    ..baumannii clinical isolates and 22 avirulent isolates from a healthy individual were subjected to multilocus sequence typing and polymerase chain reaction (PCR) for a variety of biofilm-associated genes...
  62. Sit P, Teh C, Idris N, Sam I, Syed Omar S, Sulaiman H, et al. Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) infection and the molecular characteristics of MRSA bacteraemia over a two-year period in a tertiary teaching hospital in Malaysia. BMC Infect Dis. 2017;17:274 pubmed publisher
    ..cassette chromosome mec (SCCmec) typing, detection of Panton-Valentine leukocidin (PVL) gene, multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE)...
  63. Mohamed E, Aly S, Halby H, Ahmed S, Zakaria A, El Asheer O. Epidemiological typing of multidrug-resistant Klebsiella pneumoniae, which causes paediatric ventilator-associated pneumonia in Egypt. J Med Microbiol. 2017;66:628-634 pubmed publisher
    ..This study provides a step on the way to understanding the genotyping and epidemiology of MDR K. pneumoniae for enhanced prevention of bacterial transmission. ..
  64. Yang J, Yang S. Comparative analysis of Corynebacterium glutamicum genomes: a new perspective for the industrial production of amino acids. BMC Genomics. 2017;18:940 pubmed publisher
    ..Our proposed pipeline can provide a clear perspective, including the presumed ancestor, the strain breeding trajectory, and the genomic variations necessary to increase amino acid production in C. glutamicum. ..
  65. Peters T, Bertrand S, Björkman J, Brandal L, Brown D, Erdősi T, et al. Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015. Euro Surveill. 2017;22: pubmed publisher
    ..However, differences in assigned MLVA profiles remain between well-established protocols and should be taken into account when exchanging data. ..
  66. Pin D, Guérin Faublée V, Garreau V, Breysse F, Dumitrescu O, Flandrois J, et al. Mycobacterium species related to M. leprae and M. lepromatosis from cows with bovine nodular thelitis. Emerg Infect Dis. 2014;20:2111-4 pubmed publisher
    ..These bacteria were identified as an undescribed Mycobacterium species that was phylogenetically related to M. leprae and M. lepromatosis. ..
  67. Ohshima C, Takahashi H, Iwakawa A, Kuda T, Kimura B. A novel typing method for Listeria monocytogenes using high-resolution melting analysis (HRMA) of tandem repeat regions. Int J Food Microbiol. 2017;253:36-42 pubmed publisher
    ..evaluating our developed method, the ability of MLVA-HRMA, MLVA using capillary electrophoresis, and multilocus sequence typing (MLST) was compared for their ability to discriminate between strains...
  68. Krongdang S, Evans J, Pettis J, Chantawannakul P. Multilocus sequence typing, biochemical and antibiotic resistance characterizations reveal diversity of North American strains of the honey bee pathogen Paenibacillus larvae. PLoS ONE. 2017;12:e0176831 pubmed publisher in North America and five reference strains were investigated for genetic diversity using multilocus sequence typing (MLST)...
  69. Kim B, Kim G, Kim B, Jeon C, Jeong J, Lee S, et al. Description of Mycobacterium chelonae subsp. bovis subsp. nov., isolated from cattle (Bos taurus coreanae), emended description of Mycobacterium chelonae and creation of Mycobacterium chelonae subsp. chelonae subsp. nov. Int J Syst Evol Microbiol. 2017;67:3882-3887 pubmed publisher
    ..7?% sequence similarity with that of the M. chelonae type strain. A multilocus sequence typing analysis targeting 10 housekeeping genes, including hsp65 and rpoB, revealed a phylogenetic cluster of ..
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    We have employed whole genome sequencing to define and evaluate a core genome multilocus sequence typing (cgMLST) scheme for Acinetobacter baumannii. To define a core genome we downloaded a total of 1,573 putative A...