reverse genetics

Summary

Summary: The use of techniques that produce a functional MUTATION or an effect on GENE EXPRESSION of a specific gene of interest in order to identify the role or activity of the gene product of that gene.

Top Publications

  1. Califano A, Butte A, Friend S, Ideker T, Schadt E. Leveraging models of cell regulation and GWAS data in integrative network-based association studies. Nat Genet. 2012;44:841-7 pubmed publisher
  2. Easton A. Obituary Craig R. Pringle (1930-2015). Arch Virol. 2016;161:769-70 pubmed publisher
  3. Terada Y, Kawachi K, Matsuura Y, Kamitani W. MERS coronavirus nsp1 participates in an efficient propagation through a specific interaction with viral RNA. Virology. 2017;511:95-105 pubmed publisher
    ..In addition, reverse genetics analysis suggested the presence of a cis-acting element at the 5'-terminus of the nsp1-coding region, which ..
  4. Liu Z, Yu J, Huang X, Fan H, Li X, Deng Y, et al. Characterization of cis-Acting RNA Elements of Zika Virus by Using a Self-Splicing Ribozyme-Dependent Infectious Clone. J Virol. 2017;91: pubmed publisher
    ..Finally, potential cis-acting RNA elements in ZIKV genomic RNA were identified based on this novel reverse genetics system, and the critical role of 5'-SLA promoter and 5'-3' cyclization sequences were characterized by a ..
  5. Nakagawa K, Kobayashi Y, Ito N, Suzuki Y, Okada K, Makino M, et al. Molecular Function Analysis of Rabies Virus RNA Polymerase L Protein by Using an L Gene-Deficient Virus. J Virol. 2017;91: pubmed publisher
    ..a novel experimental tool for molecular function analysis of the RABV L protein, we established by using a reverse genetics approach an L gene-deficient RABV (Nishi-ΔL/Nluc), which infects, propagates, and correspondingly ..
  6. Tuo D, Fu L, Shen W, Li X, Zhou P, Yan P. Generation of stable infectious clones of plant viruses by using Rhizobium radiobacter for both cloning and inoculation. Virology. 2017;510:99-103 pubmed publisher
    ..coli cloning system failed repeatedly. This method is simple and efficient, and is promising for a wide application in generation of infectious clones of plant virus, especially for those which are instable in E. coli. ..
  7. Retamal M, Abed Y, Rhéaume C, Baz M, Boivin G. In vitro and in vivo evidence of a potential A(H1N1)pdm09 antigenic drift mediated by escape mutations in the haemagglutinin Sa antigenic site. J Gen Virol. 2017;98:1224-1231 pubmed publisher
    ..both the HA and NA genes of the A(H1N1)pdm09 and the six internal gene segments of A/PR/8/34 were rescued by reverse genetics. In vitro, HA inhibition and microneutralization assays showed that the HA hexa-mutant reassortant virus (RG1)..
  8. Doherty P, Thomas P. Dangerous for ferrets: lethal for humans?. BMC Biol. 2012;10:10 pubmed publisher
  9. Mutso M, Saul S, Rausalu K, Susova O, Zusinaite E, Mahalingam S, et al. Reverse genetic system, genetically stable reporter viruses and packaged subgenomic replicon based on a Brazilian Zika virus isolate. J Gen Virol. 2017;98:2712-2724 pubmed publisher
    ..Efficient reverse genetic systems, genetically stable marker viruses and packaged replicons offer significant improvements for biological studies of ZIKV infection and disease, as well as for the development of antiviral approaches...

More Information

Publications24

  1. Liu J, Cattadori I, Sim D, Eden J, Holmes E, Read A, et al. Reverse Engineering Field Isolates of Myxoma Virus Demonstrates that Some Gene Disruptions or Losses of Function Do Not Explain Virulence Changes Observed in the Field. J Virol. 2017;91: pubmed publisher
    ..Using reverse genetics, we examined the roles of six selected mutations found in Australian field isolates of MYXV that fall in ..
  2. Liu Y, Nikolaitchik O, Rahman S, Chen J, Pathak V, HU W. HIV-1 Sequence Necessary and Sufficient to Package Non-viral RNAs into HIV-1 Particles. J Mol Biol. 2017;429:2542-2555 pubmed publisher
  3. Baillon L, Merour E, Cabon J, Louboutin L, Quenault H, Touzain F, et al. A single amino acid change in the non-structural NV protein impacts the virulence phenotype of Viral hemorrhagic septicemia virus in trout. J Gen Virol. 2017;98:1181-1184 pubmed publisher
    ..VHSV strain in which the NV R116 residue has been replaced by a serine, rVHSVNVR116S, was generated by reverse genetics and used to infect juvenile trout...
  4. Gilbertson B, Ng W, Crawford S, McKimm Breschkin J, Brown L. Mouse Saliva Inhibits Transit of Influenza Virus to the Lower Respiratory Tract by Efficiently Blocking Influenza Virus Neuraminidase Activity. J Virol. 2017;91: pubmed publisher
    ..Here, we investigated the viral target of the salivary inhibitor by using reverse genetics to create hybrid viruses with some surface proteins derived from an inhibitor-sensitive strain and others ..
  5. Dubois J, Cavanagh M, Terrier O, Hamelin M, Lina B, Shi R, et al. Mutations in the fusion protein heptad repeat domains of human metapneumovirus impact on the formation of syncytia. J Gen Virol. 2017;98:1174-1180 pubmed publisher
    ..Altogether, our results support the importance of the HRA domain for an HMPV-triggered fusion mechanism and identify key residues that modulate syncytium formation. ..
  6. Mögling R, Richard M, Vliet S, Beek R, Schrauwen E, Spronken M, et al. Neuraminidase-mediated haemagglutination of recent human influenza A(H3N2) viruses is determined by arginine 150 flanking the neuraminidase catalytic site. J Gen Virol. 2017;98:1274-1281 pubmed publisher
    ..Continuous monitoring of the evolution of these viruses and potential consequences for vaccine strain selection remains important. ..
  7. Zhang W, Zhang H, Liu K, Jian G, Qi F, Si N. Large-scale identification of Gossypium hirsutum genes associated with Verticillium dahliae by comparative transcriptomic and reverse genetics analysis. PLoS ONE. 2017;12:e0181609 pubmed publisher
    ..hirsutum-V. dahliae interaction and offered a number of candidate genes as potential sources for breeding wilt-tolerance in cotton. ..
  8. Kamal R, Blanchfield K, Belser J, Music N, Tzeng W, Holiday C, et al. Inactivated H7 Influenza Virus Vaccines Protect Mice despite Inducing Only Low Levels of Neutralizing Antibodies. J Virol. 2017;91: pubmed publisher
    ..In this study, we immunized mice with whole inactivated reverse genetics reassortant (RG) viruses expressing HA and neuraminidase (NA) from 3 different H7 viruses [A/Shanghai/2/2013(..
  9. Ruedas J, Ladner J, Ettinger C, Gummuluru S, Palacios G, Connor J. Spontaneous Mutation at Amino Acid 544 of the Ebola Virus Glycoprotein Potentiates Virus Entry and Selection in Tissue Culture. J Virol. 2017;91: pubmed publisher
    ..Understanding the mechanism behind this mutation can provide insight into functional differences that exist in culture conditions and among ebolavirus glycoproteins. ..
  10. Komoto S, Kanai Y, Fukuda S, Kugita M, Kawagishi T, Ito N, et al. Reverse Genetics System Demonstrates that Rotavirus Nonstructural Protein NSP6 Is Not Essential for Viral Replication in Cell Culture. J Virol. 2017;91: pubmed publisher
    ..Here, taking advantage of a recently established plasmid-only-based reverse genetics system that allows rescue of recombinant rotaviruses entirely from cloned cDNAs, we generated NSP6-deficient ..
  11. Mohanty S, Donnelly B, Dupree P, Lobeck I, Mowery S, Meller J, et al. A Point Mutation in the Rhesus Rotavirus VP4 Protein Generated through a Rotavirus Reverse Genetics System Attenuates Biliary Atresia in the Murine Model. J Virol. 2017;91: pubmed publisher
    ..of the virus, we generated a recombinant virus with a single amino acid mutation at this site through a reverse genetics system...
  12. Guttery D, Holder A, Tewari R. Sexual development in Plasmodium: lessons from functional analyses. PLoS Pathog. 2012;8:e1002404 pubmed publisher
  13. Han K, Alhatlani B, Cho S, Lee J, Hosmillo M, Goodfellow I, et al. Identification of amino acids within norovirus polymerase involved in RNA binding and viral replication. J Gen Virol. 2017;98:1311-1315 pubmed publisher
    ..a surrogate model system for norovirus research, due to the availability of robust cell culture systems and reverse genetics. To identify amino acids involved in RNA synthesis by the viral RNA-dependent RNA polymerase (NS7), we ..
  14. Liu H, Albina E, Gil P, Minet C, de Almeida R. Two-plasmid system to increase the rescue efficiency of paramyxoviruses by reverse genetics: The example of rescuing Newcastle Disease Virus. Virology. 2017;509:42-51 pubmed publisher
    Within paramyxoviruses, conventional reverse genetics require the transfection of a minimum of four plasmids: three to reconstruct the viral polymerase complex that replicates and expresses the virus genome delivered by a fourth plasmid...
  15. Baratelli M, Pedersen L, Trebbien R, Larsen L, Jungersen G, Blanco E, et al. Identification of cross-reacting T-cell epitopes in structural and non-structural proteins of swine and pandemic H1N1 influenza A virus strains in pigs. J Gen Virol. 2017;98:895-899 pubmed publisher
    ..The majority of the identified T-cell epitopes were conserved between the examined lineages, suggesting that targeting cross-reactive T-cell epitopes could be used to improve vaccines against SwIV in SLA-1*0702-positive pigs. ..