polymerase chain reaction

Summary

Summary: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

Top Publications

  1. Lopes L, Baleotti W, Suzuki R, Fabron A, Chiba A, Vieira Filho J, et al. HNA-3 gene frequencies in Brazilians and a new polymerase chain reaction-restriction fragment length polymorphism method for HNA-3a/3b genotyping. Transfusion. 2014;54:1619-21 pubmed publisher
    ..Amerindians, 74 Japanese individuals, and 124 African Brazilians were genotyped for rs2288904 by a polymerase chain reaction (PCR)-restriction fragment length polymorphism assay...
  2. Block A, Debode F, Grohmann L, Hulin J, Taverniers I, Kluga L, et al. The GMOseek matrix: a decision support tool for optimizing the detection of genetically modified plants. BMC Bioinformatics. 2013;14:256 pubmed publisher
    ..It is flexible enough to be further updated with new information and integrated in different applications and platforms. ..
  3. Golassa L, Enweji N, Erko B, Aseffa A, Swedberg G. Detection of a substantial number of sub-microscopic Plasmodium falciparum infections by polymerase chain reaction: a potential threat to malaria control and diagnosis in Ethiopia. Malar J. 2013;12:352 pubmed publisher
    ..This study aimed to explore the presence of sub-microscopic Plasmodium falciparum infections using polymerase chain reaction (PCR). The PCR-based parasite prevalence was compared against microscopy and rapid diagnostic test (RDT)...
  4. Phumee A, Kraivichian K, Chusri S, Noppakun N, Vibhagool A, Sanprasert V, et al. Detection of Leishmania siamensis DNA in saliva by polymerase chain reaction. Am J Trop Med Hyg. 2013;89:899-905 pubmed publisher
    b>Polymerase chain reaction was used to detect Leishmania siamensis DNA from clinical samples collected from six leishmaniasis patients during 2011-2012...
  5. Van der Auwera G, Maes I, De Doncker S, Ravel C, Cnops L, Van Esbroeck M, et al. Heat-shock protein 70 gene sequencing for Leishmania species typing in European tropical infectious disease clinics. Euro Surveill. 2013;18:20543 pubmed
    ..We also provide a detailed laboratory protocol that includes a step-by-step procedure of the typing methodology, to facilitate implementation in diagnostic laboratories...
  6. Nguyen Dumont T, Pope B, Hammet F, Mahmoodi M, Tsimiklis H, Southey M, et al. Cross-platform compatibility of Hi-Plex, a streamlined approach for targeted massively parallel sequencing. Anal Biochem. 2013;442:127-9 pubmed publisher
    ..To address these limitations, we previously developed Hi-Plex, a polymerase chain reaction (PCR) massively parallel sequencing strategy for screening panels of genomic target regions...
  7. Cortés Torres N, Gonzalez y Merchand J, Gonzalez Bonilla C, García Elorriaga G. Molecular analysis of mycobacteria isolated in Mexican patients with different immunodeficiencies in a tertiary care hospital. Arch Med Res. 2013;44:562-9 pubmed publisher
    ..A great genetic diversity of MTB strains was identified. This is also the first report in Mexico describing clinically important isolates of M. simiae. ..
  8. Xie K, Yang Y. RNA-guided genome editing in plants using a CRISPR-Cas system. Mol Plant. 2013;6:1975-83 pubmed publisher
    ..Our results demonstrate that the CRISPR-Cas system can be exploited as a powerful tool for gene targeting and precise genome editing in plants. ..
  9. Morgan M, Chariton A, Hartley D, Court L, Hardy C. Improved inference of taxonomic richness from environmental DNA. PLoS ONE. 2013;8:e71974 pubmed publisher
  10. Khattak A, Venkatesan M, Nadeem M, Satti H, Yaqoob A, Strauss K, et al. Prevalence and distribution of human Plasmodium infection in Pakistan. Malar J. 2013;12:297 pubmed publisher
    ..This study was undertaken to determine the current prevalence and distribution of Plasmodium species across the country...

Detail Information

Publications62

  1. Lopes L, Baleotti W, Suzuki R, Fabron A, Chiba A, Vieira Filho J, et al. HNA-3 gene frequencies in Brazilians and a new polymerase chain reaction-restriction fragment length polymorphism method for HNA-3a/3b genotyping. Transfusion. 2014;54:1619-21 pubmed publisher
    ..Amerindians, 74 Japanese individuals, and 124 African Brazilians were genotyped for rs2288904 by a polymerase chain reaction (PCR)-restriction fragment length polymorphism assay...
  2. Block A, Debode F, Grohmann L, Hulin J, Taverniers I, Kluga L, et al. The GMOseek matrix: a decision support tool for optimizing the detection of genetically modified plants. BMC Bioinformatics. 2013;14:256 pubmed publisher
    ..It is flexible enough to be further updated with new information and integrated in different applications and platforms. ..
  3. Golassa L, Enweji N, Erko B, Aseffa A, Swedberg G. Detection of a substantial number of sub-microscopic Plasmodium falciparum infections by polymerase chain reaction: a potential threat to malaria control and diagnosis in Ethiopia. Malar J. 2013;12:352 pubmed publisher
    ..This study aimed to explore the presence of sub-microscopic Plasmodium falciparum infections using polymerase chain reaction (PCR). The PCR-based parasite prevalence was compared against microscopy and rapid diagnostic test (RDT)...
  4. Phumee A, Kraivichian K, Chusri S, Noppakun N, Vibhagool A, Sanprasert V, et al. Detection of Leishmania siamensis DNA in saliva by polymerase chain reaction. Am J Trop Med Hyg. 2013;89:899-905 pubmed publisher
    b>Polymerase chain reaction was used to detect Leishmania siamensis DNA from clinical samples collected from six leishmaniasis patients during 2011-2012...
  5. Van der Auwera G, Maes I, De Doncker S, Ravel C, Cnops L, Van Esbroeck M, et al. Heat-shock protein 70 gene sequencing for Leishmania species typing in European tropical infectious disease clinics. Euro Surveill. 2013;18:20543 pubmed
    ..We also provide a detailed laboratory protocol that includes a step-by-step procedure of the typing methodology, to facilitate implementation in diagnostic laboratories...
  6. Nguyen Dumont T, Pope B, Hammet F, Mahmoodi M, Tsimiklis H, Southey M, et al. Cross-platform compatibility of Hi-Plex, a streamlined approach for targeted massively parallel sequencing. Anal Biochem. 2013;442:127-9 pubmed publisher
    ..To address these limitations, we previously developed Hi-Plex, a polymerase chain reaction (PCR) massively parallel sequencing strategy for screening panels of genomic target regions...
  7. Cortés Torres N, Gonzalez y Merchand J, Gonzalez Bonilla C, García Elorriaga G. Molecular analysis of mycobacteria isolated in Mexican patients with different immunodeficiencies in a tertiary care hospital. Arch Med Res. 2013;44:562-9 pubmed publisher
    ..A great genetic diversity of MTB strains was identified. This is also the first report in Mexico describing clinically important isolates of M. simiae. ..
  8. Xie K, Yang Y. RNA-guided genome editing in plants using a CRISPR-Cas system. Mol Plant. 2013;6:1975-83 pubmed publisher
    ..Our results demonstrate that the CRISPR-Cas system can be exploited as a powerful tool for gene targeting and precise genome editing in plants. ..
  9. Morgan M, Chariton A, Hartley D, Court L, Hardy C. Improved inference of taxonomic richness from environmental DNA. PLoS ONE. 2013;8:e71974 pubmed publisher
  10. Khattak A, Venkatesan M, Nadeem M, Satti H, Yaqoob A, Strauss K, et al. Prevalence and distribution of human Plasmodium infection in Pakistan. Malar J. 2013;12:297 pubmed publisher
    ..This study was undertaken to determine the current prevalence and distribution of Plasmodium species across the country...
  11. Li Y, Segal G, Wang Q, Dooner H. Gene tagging with engineered Ds elements in maize. Methods Mol Biol. 2013;1057:83-99 pubmed publisher
    ..Enough transgenic lines are being generated to cover most of the maize genome, allowing the targeted tagging of most genes from a Ds-launching platform located nearby. ..
  12. Chaouch M, Mhadhbi M, Adams E, Schoone G, Limam S, Gharbi Z, et al. Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of Leishmania infantum in canine leishmaniasis based on cysteine protease B genes. Vet Parasitol. 2013;198:78-84 pubmed publisher
    ..infantum specific LAMP alongside other diagnostics tools for, CanL. Our results indicate a suitable sensitivity and specificity for the developed LAMP assay that could, has usefulness application on dogs and human L. infantum diagnosis...
  13. Shin H, Lee S, Hong S, Kim S, Choi Y, Park H, et al. Prevalence of Toxoplasma gondii infection in rabbits of Korea by serological tests and nested polymerase chain reaction. J Vet Med Sci. 2013;75:1609-13 pubmed
    ..gondii-positive sera at 20, 28, 30, 35, 63 and 77 kDa. Nested polymerase chain reaction (PCR) of whole-blood samples detected the T. gondii B1 gene in 23 rabbits (16.2%)...
  14. Nelms B, Macedo P, Kothera L, Savage H, Reisen W. Overwintering biology of Culex (Diptera: Culicidae) mosquitoes in the Sacramento Valley of California. J Med Entomol. 2013;50:773-90 pubmed
    ..Therefore, both vertically and horizontally infected Culex females may persist through winter and possibly transmit WNV after diapause termination in late winter or early spring in the Sacramento Valley of California...
  15. Jiang H, Huang S, Zhou D, Zhang X, Su C, Deng S, et al. Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP. Parasit Vectors. 2013;6:227 pubmed publisher
    ..gondii isolates from pigs in Jiangxi, Sichuan, Guangdong Provinces and Chongqing Municipality in China using multilocous polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology.
  16. Cho P, Na B, Choi K, Kim J, Cho S, Lee W, et al. Development of a polymerase chain reaction applicable to rapid and sensitive detection of Clonorchis sinensis eggs in human stool samples. Pathog Glob Health. 2013;107:253-9 pubmed publisher
    ..In this study, we developed a rapid and sensitive polymerase chain reaction (PCR)-based molecular diagnostic method for detection of Clonorchis sinensis eggs in stool samples...
  17. Woyke T, Doud D, Schulz F. The trajectory of microbial single-cell sequencing. Nat Methods. 2017;14:1045-1054 pubmed publisher
  18. Raro O, Gallo S, Ferreira C, Oliveira S. Carbapenem-resistant Acinetobacter baumannii contamination in an intensive care unit. Rev Soc Bras Med Trop. 2017;50:167-172 pubmed publisher
    ..baumannii isolates. Such data highlight the ICU environment as a potential origin for the persistence of MDR A. baumannii, and hence the ICU may be a source of hospital-acquired infections caused by this microorganism. ..
  19. Parada Sanchez M, Chu E, Cox L, Undurty S, Standley J, Murray J, et al. Disrupted IRF6-NME1/2 Complexes as a Cause of Cleft Lip/Palate. J Dent Res. 2017;96:1330-1338 pubmed publisher
    ..The data presented suggest an important role for cytoplasmic IRF6 in regulating the availability or localization of the NME1/2 complex and thus the dynamic behavior of epithelia during lip/palate development. ..
  20. Williams R, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed A, et al. Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo. Development. 2018;145: pubmed publisher
  21. Einarson O, Sen D. Self-biotinylation of DNA G-quadruplexes via intrinsic peroxidase activity. Nucleic Acids Res. 2017;45:9813-9822 pubmed publisher
    ..The biotinylated DNA, moreover, remains amenable to polymerase chain reaction amplification, rendering it suitable for analysis by ChIP-Seq and related methods...
  22. Padala C, Tupurani M, Puranam K, Gantala S, Shyamala N, Kondapalli M, et al. Synergistic effect of collagenase-1 (MMP1), stromelysin-1 (MMP3) and gelatinase-B (MMP9) gene polymorphisms in breast cancer. PLoS ONE. 2017;12:e0184448 pubmed publisher
    ..MMP3-1171 5A/6A and MMP9-1562 C/T SNPs were genotyped by Allele specific polymerase chain reaction and MMP1-1607 1G/2G polymorphism by restriction fragment length polymorphism assays respectively, in 300 ..
  23. Needell J, Ir D, Robertson C, Kroehl M, Frank D, Zipris D. Maternal treatment with short-chain fatty acids modulates the intestinal microbiota and immunity and ameliorates type 1 diabetes in the offspring. PLoS ONE. 2017;12:e0183786 pubmed publisher
    ..These data demonstrate that short-chain fatty acids can reshape the intestinal microbiota and prevent virus-induced islet autoimmunity and may therefore represent a useful therapeutic strategy for disease prevention. ..
  24. Kushwaha N, Bhardwaj M, Chakraborty S. The replication initiator protein of a geminivirus interacts with host monoubiquitination machinery and stimulates transcription of the viral genome. PLoS Pathog. 2017;13:e1006587 pubmed publisher
  25. Americo J, Earl P, Moss B. Droplet digital PCR for rapid enumeration of viral genomes and particles from cells and animals infected with orthopoxviruses. Virology. 2017;511:19-22 pubmed publisher
    Droplet digital polymerase chain reaction (ddPCR) was adapted for quantifying the number of orthopoxviral genomes in purified virus samples, infected cell lysates and tissues of infected animals...
  26. Chaine M, Gubbels S, Voldstedlund M, Kristensen B, Nielsen J, Andersen L, et al. Description and validation of a new automated surveillance system for Clostridium difficile in Denmark. Epidemiol Infect. 2017;145:2594-2602 pubmed publisher
    ..This nationwide electronic system can greatly strengthen surveillance and research in various applications...
  27. Wang W, Zhang T, Wu C, Wang S, Wang Y, Li H, et al. Immortalization of chicken preadipocytes by retroviral transduction of chicken TERT and TR. PLoS ONE. 2017;12:e0177348 pubmed publisher
  28. Chung T, Cheung T, Yim S, Yu M, Chiu R, Lo K, et al. Liquid biopsy of PIK3CA mutations in cervical cancer in Hong Kong Chinese women. Gynecol Oncol. 2017;146:334-339 pubmed publisher
  29. Fukushima S, Endo M, Matsumoto Y, Fukushi J, Matsunobu T, Kawaguchi K, et al. Hypoxia-inducible factor 1 alpha is a poor prognostic factor and potential therapeutic target in malignant peripheral nerve sheath tumor. PLoS ONE. 2017;12:e0178064 pubmed publisher
    ..Finally, chetomin, an inhibitor of HIF-1α, effectively inhibited the growth of MPNST cells and induced their apoptosis. Inhibition of HIF-1α signaling is a potential treatment option for MPNSTs. ..
  30. Tostes R, Dias R, Martinele I, Senra M, D Agosto M, Massard C. Multidisciplinary re-description of Plasmodium (Novyella) paranucleophilum in Brazilian wild birds of the Atlantic Forest kept in captivity. Parasitol Res. 2017;116:1887-1897 pubmed publisher
    ..The true depth of diversity in Brazilian avian haemosporidians remains largely unknown, and given the enormous variety of vectors and avian species, there may be many more species of these blood parasites yet to be described. ..
  31. Chung D, Frausto R, Lin B, Hanser E, Cohen Z, Aldave A. Transcriptomic Profiling of Posterior Polymorphous Corneal Dystrophy. Invest Ophthalmol Vis Sci. 2017;58:3202-3214 pubmed publisher
    ..The expression of selected differentially expressed genes was validated by quantitative polymerase chain reaction (qPCR) and/or assessed by in situ hybridization in the corneal endothelium of four independent cases of ..
  32. Shakiba M, Hashemi M, Mahdar S, Rahbari Z, Bahari G. Evaluation of VNTR polymorphisms of dopamine transporter gene and the risk of bipolar disorder in Zahedan, southeast Iran. Mol Biol Res Commun. 2017;6:123-127 pubmed publisher
    ..Genotyping of the variant was done by polymerase chain reaction method...
  33. Shaw G, Liu A, Weng C, Chou C, Wang D. Inferring microbial interactions in thermophilic and mesophilic anaerobic digestion of hog waste. PLoS ONE. 2017;12:e0181395 pubmed publisher
    ..This research is still in its infancy, but it can help to depict the dynamics of microbial ecosystems and to lay the groundwork for understanding how microorganisms cohabit in the anaerobic digester. ..
  34. Huo Z, Luo X, Zhan X, Chu Q, Xu Q, Yao J, et al. Genetic analysis of indel markers in three loci associated with Parkinson's disease. PLoS ONE. 2017;12:e0184269 pubmed publisher
    ..After stratification by gender, no significant differences were observed in any indels. These results indicate that the GIGYF2 indel may be associated with increased risk of PD in northern China. ..
  35. Kang J, Lee J, Kim T, Koh E, Kim T, Sohn W, et al. PCR-RFLP for Rapid Subtyping of Plasmodium vivax Korean Isolates. Korean J Parasitol. 2017;55:159-165 pubmed publisher
    ..vivax and to supervise the genetic variation of the parasite circulating in Korea. ..
  36. Koosha M, Oshaghi M, Sedaghat M, Vatandoost H, Azari Hamidian S, Abai M, et al. Sequence analysis of mtDNA COI barcode region revealed three haplotypes within Culex pipiens assemblage. Exp Parasitol. 2017;181:102-110 pubmed publisher
  37. Bhate R, Pansare N, Chaudhari S, Barbuddhe S, Choudhary V, Kurkure N, et al. Prevalence and Phylogenetic Analysis of Orientia tsutsugamushi in Rodents and Mites from Central India. Vector Borne Zoonotic Dis. 2017;17:749-754 pubmed publisher
    ..tsutsugamushi using PCR targeting 56?kDa outer membrane protein gene. Thus, perpetuation of O. tsutsugamushi among rodents and mites was detected constituting a potential public health concern...
  38. Paquette M, Dufour R, Baass A. Scavenger Receptor LOX1 Genotype Predicts Coronary Artery Disease in Patients With Familial Hypercholesterolemia. Can J Cardiol. 2017;33:1312-1318 pubmed publisher
    ..Carrying the C allele of the rs11053646 variant of the OLR1 gene was associated with an increased risk of CAD in heterozygous adult patients with FH, and this risk could be even greater in smokers as well as in younger patients. ..
  39. Li L, Zheng S, Brinckmann J, Fu J, Zeng R, Huang L, et al. Chemical and genetic diversity of Astragalus mongholicus grown in different eco-climatic regions. PLoS ONE. 2017;12:e0184791 pubmed publisher
  40. Wang L, Shen J, Cram D, Ma M, Wang H, Zhang W, et al. Preferential selection and transfer of euploid noncarrier embryos in preimplantation genetic diagnosis cycles for reciprocal translocations. Fertil Steril. 2017;108:620-627.e4 pubmed publisher
    ..Definition of translocation breakpoints and polymerase chain reaction (PCR) diagnostic primers, correct diagnosis of euploid embryos for carrier status, and a live birth with ..
  41. Gassner C, Brönnimann C, Merki Y, Mattle Greminger M, Sigurdardottir S, Meyer E, et al. Stepwise partitioning of Xp21: a profiling method for XK deletions causative of the McLeod syndrome. Transfusion. 2017;57:2125-2135 pubmed publisher
    ..Three different McLeod patient samples were tested using 16 initial positional polymerase chain reaction (PCR) procedures distributed over an approximately 2...
  42. Mustafa A, Habibi N, Osman A, Shaheed F, Khan M. Species identification and molecular typing of human Brucella isolates from Kuwait. PLoS ONE. 2017;12:e0182111 pubmed publisher
    ..Furthermore, the combined minimum spanning tree analysis demonstrated that, compared to MLVA types discovered all over the world, the Kuwaiti isolates were a distinct group of MLVA-11 and MLVA-16 types in the East Mediterranean Region. ..
  43. Zendehboodi Z. Association of glutathione S-transferase M1 and T1 polymorphisms and temperament. Mol Biol Res Commun. 2017;6:95-100 pubmed publisher
    ..The GSTM1 and GSTT1 genotypes were determined using a polymerase chain reaction (PCR)-based method...
  44. Eryildiz C, Bukavaz S, Gurcan S, Hatipoglu O. [A rarely isolated bacterium in microbiology laboratories: Streptococcus uberis]. Mikrobiyol Bul. 2017;51:171-176 pubmed
    ..spacer gene regions were amplified with specific primers and partial DNA sequence analysis of 16S rRNA polymerase chain reaction (PCR) products were performed by 3500xL Genetic Analyzer (Applied Biosystems, USA)...
  45. Arbab M, Tahir S, Niazi M, Ishaq M, Hussain A, Siddique P, et al. TNF-α Genetic Predisposition and Higher Expression of Inflammatory Pathway Components in Keratoconus. Invest Ophthalmol Vis Sci. 2017;58:3481-3487 pubmed publisher
    ..Quantitative polymerase chain reaction (qPCR) was carried out to compare the mRNA expression of TNF-α, TNFR1, TNFR2, RELA, and IL6 in the ..
  46. Husain H, Velculescu V. Cancer DNA in the Circulation: The Liquid Biopsy. JAMA. 2017;318:1272-1274 pubmed publisher
  47. Lopez J, Lorenzo L, Alcántara R, Navas J. Characterization of Aliivibrio fischeri strains associated with disease outbreak in brill Scophthalmus rhombus. Dis Aquat Organ. 2017;124:215-222 pubmed publisher
  48. Chua T, Manin B, Daim S, Vythilingam I, Drakeley C. Phylogenetic analysis of simian Plasmodium spp. infecting Anopheles balabacensis Baisas in Sabah, Malaysia. PLoS Negl Trop Dis. 2017;11:e0005991 pubmed publisher
    ..Presently, knowlesi malaria is the only zoonotic simian malaria in Malaysia with a high prevalence recorded in the states of Sabah and Sarawak...
  49. Yang L, Ji Y, Chen L, Li M, Wu F, Hu J, et al. Genetic variability in LMP2 and LMP7 is associated with the risk of esophageal squamous cell carcinoma in the Kazakh population but is not associated with HPV infection. PLoS ONE. 2017;12:e0186319 pubmed publisher
    ..b>Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was performed to detect LMP2/LMP7 gene ..
  50. Christenson E, Dalton W, Chu D, Waters I, Cravero K, Zabransky D, et al. Single-Nucleotide Polymorphism Leading to False Allelic Fraction by Droplet Digital PCR. Clin Chem. 2017;63:1370-1376 pubmed publisher
  51. Yin C, Yao Y, Yan Z, Yang H. [Correlation between mutation of p53 gene 2-4 exons from peripheral blood and HPV16 positive cervical cancer susceptibility and clinical significance]. Zhonghua Fu Chan Ke Za Zhi. 2017;52:320-326 pubmed publisher
    ..029]???????????????????????????????????????????????????????????p>0.05?? ??? ????p53??2~4??????hpv16?????????????????????c?a2?????gg?a1a2????c-a2?g-a1???????????????????????????????p53???????????????????????????????. ..
  52. Hannaoui S, Amidian S, Cheng Y, Duque Velasquez C, Dorosh L, Law S, et al. Destabilizing polymorphism in cervid prion protein hydrophobic core determines prion conformation and conversion efficiency. PLoS Pathog. 2017;13:e1006553 pubmed publisher
    ..Therefore, a thorough analysis of CWD isolates and re-assessment of species-barriers is important in order to fully exclude a zoonotic potential of CWD. ..
  53. Kubota Aizawa S, Ohno K, Fukushima K, Kanemoto H, Nakashima K, Uchida K, et al. Epidemiological study of gastric Helicobacter spp. in dogs with gastrointestinal disease in Japan and diversity of Helicobacter heilmannii sensu stricto. Vet J. 2017;225:56-62 pubmed publisher
    ..detected in canine stomachs in our study were most closely related to H. heilmannii s.s. and displayed substantial genetic diversity. Infection with Helicobacter spp. may be associated with more severe gastritis in dogs. ..
  54. Moldal T, Vikøren T, Cliquet F, Marston D, Van Der Kooij J, Madslien K, et al. First detection of European bat lyssavirus type 2 (EBLV-2) in Norway. BMC Vet Res. 2017;13:216 pubmed publisher
    ..EBLV-2 was detected in brain tissues by polymerase chain reaction (PCR) followed by sequencing of a part of the nucleoprotein gene, and lyssavirus was isolated in ..
  55. Dave G. A rapid qualitative assay for detection of Clostridium perfringens in canned food products. Acta Biochim Pol. 2017;64:207-213 pubmed publisher
    ..tetani, C. botulinum, C. acetobutyricum, Bacillus subtilis, and Escherichia coli. This assay is extramly rapid and provides reliable and reproducible results within one hour of incubation at 37°C. ..
  56. Maheshwari Y, Selvaraj V, Hajeri S, Yokomi R. Application of droplet digital PCR for quantitative detection of Spiroplasma citri in comparison with real time PCR. PLoS ONE. 2017;12:e0184751 pubmed publisher
    Droplet digital polymerase chain reaction (ddPCR) is a method for performing digital PCR that is based on water-oil emulsion droplet technology...
  57. Boonkasidecha S, Kannan P, Kallapur S, Jobe A, Kemp M. Fetal skin as a pro-inflammatory organ: Evidence from a primate model of chorioamnionitis. PLoS ONE. 2017;12:e0184938 pubmed publisher
    ..These findings provide support for the role of the fetal skin in the development of the fetal inflammatory response. ..
  58. Sakihama S, Saito M, Kuba Miyara M, Tomoyose T, Taira N, Miyagi T, et al. Human T-cell leukemia virus type I Tax genotype analysis in Okinawa, the southernmost and remotest islands of Japan: Different distributions compared with mainland Japan and the potential value for the prognosis of aggressive adult T-cell leukemia/ly. Leuk Res. 2017;61:18-24 pubmed publisher
    ..68 (95% confidence interval, 0.87-8.25; P=0.086). Our results suggest that the tax genotype has clinical value as a prognostic factor for aggressive ATL. ..
  59. Panneum S, Rukkwamsuk T. Diagnosis of Caprine Arthritis Encephalitis Virus infection in dairy goats by ELISA, PCR and Viral Culture. Pol J Vet Sci. 2017;20:347-353 pubmed publisher
  60. Dengo Baloi L, Semá Baltazar C, Manhique L, Chitio J, Inguane D, Langa J. Antibiotics resistance in El Tor Vibrio cholerae 01 isolated during cholera outbreaks in Mozambique from 2012 to 2015. PLoS ONE. 2017;12:e0181496 pubmed publisher
    ..Continuous monitoring of antibiotic resistance pattern of epidemic strains is therefore crucial since the appearance of antibiotic resistance can influence cholera control strategies. ..
  61. Singh G, Sithebe A, Enitan A, Kumari S, Bux F, Stenström T. Comparison of droplet digital PCR and quantitative PCR for the detection of Salmonella and its application for river sediments. J Water Health. 2017;15:505-508 pubmed publisher
    Despite advances in microbial detection that quantitative polymerase chain reaction (qPCR) has led to, complex environmental samples, such as sediments, remain a challenge due to presence of PCR inhibitors...
  62. Joanne S, Vythilingam I, Teoh B, Leong C, Tan K, Wong M, et al. Vector competence of Malaysian Aedes albopictus with and without Wolbachia to four dengue virus serotypes. Trop Med Int Health. 2017;22:1154-1165 pubmed publisher
    ..albopictus is susceptible to dengue virus infections and capable of transmitting dengue virus, especially DENV-1 and DENV-4. Removal of Wolbachia from Malaysian Ae. albopictus would not reduce their susceptibility status. ..