amplified fragment length polymorphism analysis


Summary: The detection of RESTRICTION FRAGMENT LENGTH POLYMORPHISMS by selective PCR amplification of restriction fragments derived from genomic DNA followed by electrophoretic analysis of the amplified restriction fragments.

Top Publications

  1. Zhan Z, Nwafor C, Hou Z, Gong J, Zhu B, Jiang Y, et al. Cytological and morphological analysis of hybrids between Brassicoraphanus, and Brassica napus for introgression of clubroot resistant trait into Brassica napus L. PLoS ONE. 2017;12:e0177470 pubmed publisher
    ..napus clubroot resistant cultivars. ..
  2. Segatto A, Reck Kortmann M, Turchetto C, Freitas L. Multiple markers, niche modelling, and bioregions analyses to evaluate the genetic diversity of a plant species complex. BMC Evol Biol. 2017;17:234 pubmed publisher
    ..integrifolia complex represents a continuum of individuals from distant and historically isolated populations, which share some morphological traits, but are established in four different evolutionary lineages. ..
  3. Van der Auwera G, Bhattarai N, Odiwuor S, Vuylsteke M. Remarks on identification of amplified fragment length polymorphisms linked to SAG resistance in Leishmania. Acta Trop. 2010;113:92-3 pubmed publisher
  4. Listl D, Poschlod P, Reisch C. Phylogeography of a tough rock survivor in European dry grasslands. PLoS ONE. 2017;12:e0179961 pubmed publisher
    ..Therefore, central European grasslands were recently colonized by northern cryptic populations and source populations originating in the east and the Apennine Peninsula. ..
  5. Wondimu T, Gizaw A, Tusiime F, Masao C, Abdi A, Hou Y, et al. Genetic structuring, dispersal and taxonomy of the high-alpine populations of the Geranium arabicum/kilimandscharicum complex in tropical eastern Africa. PLoS ONE. 2017;12:e0178208 pubmed publisher
    ..arabicum in Ethiopia. Interestingly, the high-alpine G. kilimandscharicum lacked clear geographic structuring, suggesting a recent history of colonization of the different mountains or extensive intermountain gene flow. ..
  6. Araki K, Kubo T, Kudoh H. Genet-specific DNA methylation probabilities detected in a spatial epigenetic analysis of a clonal plant population. PLoS ONE. 2017;12:e0178145 pubmed publisher
    ..This finding suggests that it requires further studies on the spatial epigenetic structure of natural populations of diverse organisms, particularly for sessile clonal species. ..
  7. Magri M, Prata T, Manchiero C, Dantas B, Mazza C, Tengan F. Genetic variation in the microsomal triglyceride transfer protein (-493G/T) is associated with hepatic steatosis in patients infected with hepatitis C virus. BMC Infect Dis. 2017;17:235 pubmed publisher
    ..Furthermore, associations were observed between hepatic steatosis and inflammatory activity of moderate to high intensity, advanced age, elevated GGT and low LDL levels. ..
  8. Lee G, Lee J, Lim K, Suh I, Ryu S, Eom Y, et al. Prevalence of multidrug-resistant Acinetobacter baumannii producing OXA-23-like from a healthcare facility of Gangwon Province, South Korea. Int J Antimicrob Agents. 2012;39:452-3 pubmed publisher
  9. Kumar A, Prakash A, Singh A, Kumar H, Hagen F, Meis J, et al. Candida haemulonii species complex: an emerging species in India and its genetic diversity assessed with multilocus sequence and amplified fragment-length polymorphism analyses. Emerg Microbes Infect. 2016;5:e49 pubmed publisher

More Information


  1. Li J, Wang D, Xu X, Bai L, Peng B, Pu Y, et al. Utilization of UPLC/Q-TOF-MS-Based Metabolomics and AFLP-Based Marker-Assisted Selection to Facilitate/Assist Conventional Breeding of Polygala tenuifolia. Chem Biodivers. 2017;14: pubmed publisher
    ..The proposed method of metabolomics coupled with MAS could facilitate/assist the breeding of P. tenuifolia. ..
  2. Vašek J, Hlásná Čepková P, Viehmannová I, Ocelák M, Cachique Huansi D, Vejl P. Dealing with AFLP genotyping errors to reveal genetic structure in Plukenetia volubilis (Euphorbiaceae) in the Peruvian Amazon. PLoS ONE. 2017;12:e0184259 pubmed publisher
    ..The study illustrates the successful application of AFLP to infer genetic structure in non-model plants. ..
  3. Aiba T, Saito T, Hayashi A, Sato S, Yunokawa H, Maruyama T, et al. Methylated site display (MSD)-AFLP, a sensitive and affordable method for analysis of CpG methylation profiles. BMC Mol Biol. 2017;18:7 pubmed publisher
    ..Regarding the remarkable precision, sensitivity, and throughput of MSD-AFLP analysis studies, this method will be advantageous in a variety of epigenetics-based research. ..
  4. Zeng Y, Ye S, Yu W, Wu S, Hou W, Wu R, et al. Genetic linkage map construction and QTL identification of juvenile growth traits in Torreya grandis. BMC Genet. 2014;15 Suppl 1:S2 pubmed publisher
    ..It was observed that the genetic effects of QTLs on growth traits vary with age, suggesting the dynamic behavior of growth QTLs. Part of the QTLs was found to display a pleiotropic effect on basal diameter growth and height growth. ..
  5. Cai D, Xiao Y, Yang W, Ye W, Wang B, Younas M, et al. Association mapping of six yield?related traits in rapeseed (Brassica napus L.). Theor Appl Genet. 2014;127:85-96 pubmed
    ..The combination of favorable alleles of multiple associated markers significantly enhances trait performance, illustrating a great potential of utilization of the associations in rapeseed breeding programs. ..
  6. Ney G, Schul J. Population structure within the one-dimensional range of a coastal plain katydid. PLoS ONE. 2017;12:e0179361 pubmed publisher
    ..We also observed a change in body size across the range. Body size, as measured by male hind femur length, correlated closely with latitude, a possible indication of differential selection across the species range. ..
  7. Bodhini D, Radha V, Ghosh S, Majumder P, Mohan V. Lack of association of PTPN1 gene polymorphisms with type 2 diabetes in south Indians. J Genet. 2011;90:323-6 pubmed
  8. Wulff T, Argüello R, Molina Jordàn M, Roura Frigolé H, Hauquier G, Filonava L, et al. Detection of a Subset of Posttranscriptional Transfer RNA Modifications in Vivo with a Restriction Fragment Length Polymorphism-Based Method. Biochemistry. 2017;56:4029-4038 pubmed publisher
    ..For the examples studied, we show that this approach can reliably estimate the modification status of the sample, a feature that can be useful in the study of the regulatory role of tRNA modifications in gene expression. ..
  9. Wang J, Su K, Guo Y, Xing H, Zhao Y, Liu Z, et al. Construction of a high-density genetic map for grape using specific length amplified fragment (SLAF) sequencing. PLoS ONE. 2017;12:e0181728 pubmed publisher
  10. Zhang B, Shang P, Tao Z, Qiangba Y, Wang Z, Zhang H. Effect of a single nucleotide polymorphism in the growth hormone secretagogue receptor (GHSR) gene on growth rate in pigs. Gene. 2017;634:68-73 pubmed publisher
    ..05). The results indicated that GHSR had a positive influence on the growth rate of pigs and suggested that the C-1595A SNP could be of value as a molecular marker for improving the production performance of pig breeds. ..
  11. Richards C, Bossdorf O, Verhoeven K. Understanding natural epigenetic variation. New Phytol. 2010;187:562-4 pubmed publisher
  12. Guo P, Qi Y, Yang L, Ye X, Jiang H, Huang J, et al. cDNA-AFLP analysis reveals the adaptive responses of citrus to long-term boron-toxicity. BMC Plant Biol. 2014;14:284 pubmed publisher
    ..grandis ones, thus improving the B-tolerance of C. sinensis plants. Our findings reveal some novel mechanisms on the tolerance of plants to B-toxicity at the gene expression level. ..