Genomes and Genes
cellular reprogramming techniques
Summary: Procedures used for the induction of CELLULAR REPROGRAMMING to change the terminal phenotype of a cell, such as the generation of INDUCED PLURIPOTENT STEM CELLS from differentiated adult cells by the forced expression of specific genes.
- Hirota T, Ohta H, Powell B, Mahadevaiah S, Ojarikre O, Saitou M, et al. Fertile offspring from sterile sex chromosome trisomic mice. Science. 2017;357:932-935 pubmed publisher..It can also create euploid iPSCs from human trisomic patient fibroblasts. The findings have relevance to overcoming infertility and other trisomic phenotypes. ..
- Rocchetti M, Sala L, Dreizehnter L, Crotti L, Sinnecker D, Mura M, et al. Elucidating arrhythmogenic mechanisms of long-QT syndrome CALM1-F142L mutation in patient-specific induced pluripotent stem cell-derived cardiomyocytes. Cardiovasc Res. 2017;113:531-541 pubmed publisher..Impaired CDI of ICaL underlined the electrical abnormality, which was sensitive to ICaL blockade. High mutation penetrance was confirmed in the presence of the native genotype, implying strong dominance of effects. ..
- Ellis C, Ramzy A, Kieffer T. Regenerative medicine and cell-based approaches to restore pancreatic function. Nat Rev Gastroenterol Hepatol. 2017;14:612-628 pubmed publisher..We also discuss the tools and strategies being used to generate exocrine pancreas and insulin-producing ?-cell surrogates in situ and highlight obstacles to clinical application. ..
- Rhee C, Lee B, Beck S, LeBlanc L, Tucker H, Kim J. Mechanisms of transcription factor-mediated direct reprogramming of mouse embryonic stem cells to trophoblast stem-like cells. Nucleic Acids Res. 2017;45:10103-10114 pubmed publisher..Therefore, we reveal that CAG factors function as both decommission and pioneer factors during ES to TS-like cell fate conversion. ..