cellular reprogramming techniques


Summary: Procedures used for the induction of CELLULAR REPROGRAMMING to change the terminal phenotype of a cell, such as the generation of INDUCED PLURIPOTENT STEM CELLS from differentiated adult cells by the forced expression of specific genes.

Top Publications

  1. Hirano K, Konagaya S, Turner A, Noda Y, Kitamura S, Kotera H, et al. Closed-channel culture system for efficient and reproducible differentiation of human pluripotent stem cells into islet cells. Biochem Biophys Res Commun. 2017;487:344-350 pubmed publisher
    ..Our system shows an easily scalable, novel method for inducing PSC differentiation with both purity and functionality. ..
  2. Teshigawara R, Cho J, Kameda M, Tada T. Mechanism of human somatic reprogramming to iPS cell. Lab Invest. 2017;97:1152-1157 pubmed publisher
    ..Applications of genome-editing technology to pluripotent stem cells will reshape our approaches for exploring molecular mechanisms. ..
  3. Han M, Kim H, O Reilly C, Kim C. Purification of functional reprogramming factors in mammalian cell using FLAG -Tag. Biochem Biophys Res Commun. 2017;492:154-160 pubmed publisher
    ..Cell Stem Cells, 2009]. In here, we show that the expression of reprogramming factors in mammalian cells and subsequent purification of these factors by FLAG-Tag could reprogram fibroblasts into iPSCs. ..
  4. Skamagki M, Correia C, Yeung P, Baslan T, Beck S, Zhang C, et al. ZSCAN10 expression corrects the genomic instability of iPSCs from aged donors. Nat Cell Biol. 2017;19:1037-1048 pubmed publisher
    ..Correcting the genomic instability of A-iPSCs will ultimately enhance our ability to produce histocompatible functional tissues from older patients' own cells that are safe for transplantation. ..
  5. Ma H, Wang L, Yin C, Liu J, Qian L. In vivo cardiac reprogramming using an optimal single polycistronic construct. Cardiovasc Res. 2015;108:217-9 pubmed publisher
  6. Weinberg B, Pham N, Caraballo L, Lozanoski T, Engel A, Bhatia S, et al. Large-scale design of robust genetic circuits with multiple inputs and outputs for mammalian cells. Nat Biotechnol. 2017;35:453-462 pubmed publisher
    ..BLADE enables execution of sophisticated cellular computation in mammalian cells, with applications in cell and tissue engineering. ..
  7. CieĊ›lar Pobuda A, Knoflach V, Ringh M, Stark J, Likus W, Siemianowicz K, et al. Transdifferentiation and reprogramming: Overview of the processes, their similarities and differences. Biochim Biophys Acta Mol Cell Res. 2017;1864:1359-1369 pubmed publisher
    ..This process involves an ectopic expression of transcription factors and/or other stimuli. Unlike in the case of reprogramming, tissues obtained by this method do not carry the risk of subsequent teratomagenesis. ..
  8. Ezati R, Etemadzadeh A, Soheili Z, Samiei S, Ranaei Pirmardan E, Davari M, et al. The influence of rAAV2-mediated SOX2 delivery into neonatal and adult human RPE cells; a comparative study. J Cell Physiol. 2018;233:1222-1235 pubmed publisher
    ..However, the increased number of PAX6, CHX10, Thy1, and rhodopsin positive cells in adult hRPE treated cultures clearly indicated the considerable generation of neuro-retinal terminally differentiated cells. ..
  9. Bektik E, Dennis A, Prasanna P, Madabhushi A, Fu J. Single cell qPCR reveals that additional HAND2 and microRNA-1 facilitate the early reprogramming progress of seven-factor-induced human myocytes. PLoS ONE. 2017;12:e0183000 pubmed publisher
    ..Our study provides valuable information to further optimize a method of direct iCM-reprogramming in human cells. ..

More Information


  1. Hirota T, Ohta H, Powell B, Mahadevaiah S, Ojarikre O, Saitou M, et al. Fertile offspring from sterile sex chromosome trisomic mice. Science. 2017;357:932-935 pubmed publisher
    ..It can also create euploid iPSCs from human trisomic patient fibroblasts. The findings have relevance to overcoming infertility and other trisomic phenotypes. ..
  2. Rocchetti M, Sala L, Dreizehnter L, Crotti L, Sinnecker D, Mura M, et al. Elucidating arrhythmogenic mechanisms of long-QT syndrome CALM1-F142L mutation in patient-specific induced pluripotent stem cell-derived cardiomyocytes. Cardiovasc Res. 2017;113:531-541 pubmed publisher
    ..Impaired CDI of ICaL underlined the electrical abnormality, which was sensitive to ICaL blockade. High mutation penetrance was confirmed in the presence of the native genotype, implying strong dominance of effects. ..
  3. Ellis C, Ramzy A, Kieffer T. Regenerative medicine and cell-based approaches to restore pancreatic function. Nat Rev Gastroenterol Hepatol. 2017;14:612-628 pubmed publisher
    ..We also discuss the tools and strategies being used to generate exocrine pancreas and insulin-producing ?-cell surrogates in situ and highlight obstacles to clinical application. ..
  4. Galivo F, Benedetti E, Wang Y, Pelz C, Schug J, Kaestner K, et al. Reprogramming human gallbladder cells into insulin-producing ?-like cells. PLoS ONE. 2017;12:e0181812 pubmed publisher
  5. Kim H, Yoo J, Shin J, Chang Y, Jung J, Jo D, et al. Modelling APOE ?3/4 allele-associated sporadic Alzheimer's disease in an induced neuron. Brain. 2017;140:2193-2209 pubmed publisher
  6. Rhee C, Lee B, Beck S, LeBlanc L, Tucker H, Kim J. Mechanisms of transcription factor-mediated direct reprogramming of mouse embryonic stem cells to trophoblast stem-like cells. Nucleic Acids Res. 2017;45:10103-10114 pubmed publisher
    ..Therefore, we reveal that CAG factors function as both decommission and pioneer factors during ES to TS-like cell fate conversion. ..