semen analysis


Summary: The quality of SEMEN, an indicator of male fertility, can be determined by semen volume, pH, sperm concentration (SPERM COUNT), total sperm number, sperm viability, sperm vigor (SPERM MOTILITY), normal sperm morphology, ACROSOME integrity, and the concentration of WHITE BLOOD CELLS.

Top Publications

  1. Zhang J, Liu J, Ren L, Wei J, Duan J, Zhang L, et al. PM2.5 induces male reproductive toxicity via mitochondrial dysfunction, DNA damage and RIPK1 mediated apoptotic signaling pathway. Sci Total Environ. 2018;634:1435-1444 pubmed publisher
    ..The above three aspects together resulted in the decrease in sperm quantity and quality. ..
  2. Dardmeh F, Alipour H, Gazerani P, van der Horst G, Brandsborg E, Nielsen H. Lactobacillus rhamnosus PB01 (DSM 14870) supplementation affects markers of sperm kinematic parameters in a diet-induced obesity mice model. PLoS ONE. 2017;12:e0185964 pubmed publisher
    ..This study demonstrated the potential of L. rhamnosus PB01 as a regulatory agent with positive effects on weight loss and reproductive-hormones, significantly improving sperm motility and kinematic parameters in male DIO models. ..
  3. Jansen C, Elisen M, Leenstra C, Kaaijk E, van Stralen K, Verhoeve H. Longer time interval between semen processing and intrauterine insemination does not affect pregnancy outcome. Fertil Steril. 2017;108:764-769 pubmed publisher
    ..This approach allows additional flexibility for couples when the male partner is not available on the day of ovulation, and it allows for a spread of workload in the laboratory. ..
  4. Samplaski M, Lo K, Grober E, Zini A, Jarvi K. Varicocelectomy to "upgrade" semen quality to allow couples to use less invasive forms of assisted reproductive technology. Fertil Steril. 2017;108:609-612 pubmed publisher
    ..Even for low TMSCs, a varicocelectomy may reduce the need for IVF. Varicocele repair (by embolization or microsurgery) potentially reduces the need for IVF and IUI. ..
  5. Eisenberg M, Sapra K, Kim S, Chen Z, Buck Louis G. Semen quality and pregnancy loss in a contemporary cohort of couples recruited before conception: data from the Longitudinal Investigation of Fertility and the Environment (LIFE) Study. Fertil Steril. 2017;108:613-619 pubmed publisher
    ..However, given that 30% of pregnancies end in miscarriage and half the fetal genome is paternal in origin, the findings await corroboration. ..
  6. Wdowiak A, Bień A, Iwanowicz Palus G, Makara Studzinska M, Bojar I. Impact of emotional disorders on semen quality in men treated for infertility. Neuro Endocrinol Lett. 2017;38:50-58 pubmed
    ..Depression and anxiety in subfertile males are associated with lower secretion of SHBG and DHEA-S, and higher secretion of cortisol and prolactin. Depression and anxiety in male patients cause decreased semen volume and sperm density. ..
  7. Liu S, Yin H, Li C, Qin C, Cai W, Cao M, et al. Genetic effects of PDGFRB and MARCH1 identified in GWAS revealing strong associations with semen production traits in Chinese Holstein bulls. BMC Genet. 2017;18:63 pubmed publisher
    ..The identified SNPs may serve as genetic markers to optimize breeding programs for semen production traits in Holstein bull populations. ..
  8. Lueders I, Young D, Maree L, van der Horst G, Luther I, Botha S, et al. Effects of GnRH vaccination in wild and captive African Elephant bulls (Loxodonta africana) on reproductive organs and semen quality. PLoS ONE. 2017;12:e0178270 pubmed publisher
    ..After 2-4 years of continuous treatment every 5-7 months, the effects were similar to surgical castration. ..
  9. Pipan M, Mrkun J, Strajn B, Vrtač K, Kos J, Pišlar A, et al. The influence of macro- and microelements in seminal plasma on diluted boar sperm quality. Acta Vet Scand. 2017;59:11 pubmed publisher
    ..Our results demonstrate that the evaluation of Se in fresh boar seminal plasma can serve as an additional tool in predicting sperm quality after storage. ..

More Information


  1. Kanakasabapathy M, Sadasivam M, Singh A, Preston C, Thirumalaraju P, Venkataraman M, et al. An automated smartphone-based diagnostic assay for point-of-care semen analysis. Sci Transl Med. 2017;9: pubmed publisher
    ..Manual microscope-based testing and computer-assisted semen analysis (CASA) are the current standard methods to diagnose male infertility; however, these methods are labor-..
  2. Prochowska S, Nizanski W. In vitro fertilizing potential of urethral and epididymal spermatozoa collected from domestic cats (Felis catus). Pol J Vet Sci. 2017;20:19-24 pubmed publisher
    ..In this study we confirmed that cryopreserved urethral spermatozoa have equally good fertilizing potential as epididymal ones, and both can be successfully used for in vitro fertilization in cats with the use of commercial medium. ..
  3. Osadchuk L, Popova A, Erkovich A, Voroshilova N, Osadchuk A. [Effects of smoking and alcohol consumptionon reproductive and metabolic indicators in young men in western siberia]. Urologiia. 2017;:62-67 pubmed
    ..Thus, in young men, frequent drinking and smoking can alter the hormonal and metabolic balance, which, as the duration of the exposure and the strength of the factors increase, will increase the risk of reproductive disorders. ..
  4. Ribeiro S, Muratori M, De Geyter M, De Geyter C. TUNEL labeling with BrdUTP/anti-BrdUTP greatly underestimates the level of sperm DNA fragmentation in semen evaluation. PLoS ONE. 2017;12:e0181802 pubmed publisher
    ..As a consequence, the kit using BrdUTP/fluorescein-anti-BrdUTP should not be recommended as a method to assay DNA damage in semen. This study represents one further step in the standardization of TUNEL among laboratories. ..
  5. Ali L, Hassan Andrabi S, Ahmed H, Hussain Shah A. Freezability of water buffalo spermatozoa is improved with the addition of catalase in cryodiluent. Cryo Letters. 2017;38:145-154 pubmed
    ..05) in T4 group as compared with control. Addition of catalase at a concentration of 300IU/ml in TCA cryodiluent improved the freezability of water buffalo spermatozoa. ..
  6. Rick I, Mehlis M, Eßer E, Bakker T. The influence of ambient ultraviolet light on sperm quality and sexual ornamentation in three-spined sticklebacks (Gasterosteus aculeatus). Oecologia. 2014;174:393-402 pubmed
    ..By highlighting the role of UVA as a factor influencing fitness-relevant traits, our findings may contribute to a better understanding of the consequences of current and future levels of solar UVR for mating systems and life history. ..
  7. Sosnin D, Zubareva N, Nenasheva O, Krivtsov A, Karimova N, Pozdin N. [Ejaculate and serum procalcitonin levels in healthy men and men with oligoasthenozoospermia]. Urologiia. 2017;:61-65 pubmed
    ..Further studies seem warranted, specifically considering the role and source of procalcitonin production in sperm. ..
  8. Kably Ambe A, Carballo Mondragon E, Roque Sánchez A, Durán Monterosas L, Amaro Hernández E. [Evaluation of semen parameters in long term cryopreserved samples for over 10 years]. Ginecol Obstet Mex. 2016;84:1-6 pubmed
    ..5 ± 3.01 respectively) compared with OAT Groups and QX (9.8 ± 0.2 and 12, 17 ± 3.9). It is possible to store semen samples for a long period of time, retrieving suitable viability sperm useful for assisted reproduction techniques. ..
  9. Wei R, Chen J, Huang J, Zhang X, Cui Y. [Type of sperm DNA strand breaks in infertile men and its clinical implication]. Zhonghua Nan Ke Xue. 2015;21:604-9 pubmed
    ..05 or P < 0.01). Double-stranded, rather than single-stranded DNA breaks, may be a factor inducing male infertility. The type of sperm DNA strand damage is of much reference value for the assessment of male fertility. ..
  10. Gundersen T, Jørgensen N, Andersson A, Bang A, Nordkap L, Skakkebæk N, et al. Association Between Use of Marijuana and Male Reproductive Hormones and Semen Quality: A Study Among 1,215 Healthy Young Men. Am J Epidemiol. 2015;182:473-81 pubmed publisher
    ..Marijuana smokers had higher levels of testosterone within the same range as cigarette smokers. Our findings are of public interest as marijuana use is common and may be contributing to recent reports of poor semen quality. ..
  11. Slama R, Vrijheid M. Some challenges of studies aiming to relate the Exposome to human health. Occup Environ Med. 2015;72:383-4 pubmed publisher
  12. Gomaa A, Abou Khalil N, Abdel Ghani M. The protective role of folic acid against testicular dysfunction in lead-intoxicated rat model. Gen Physiol Biophys. 2017;36:297-308 pubmed publisher the relative testicular weight and the serum level of testosterone, an impairment in the characters of semen analysis, and an increased content of lead, malondialdehyde and both interleukin-6 and -10 and a decreased antioxidant ..
  13. SaÄŸlam H, AltundaÄŸ H, Atik Y, Dündar M, Adsan Ã. Trace elements levels in the serum, urine, and semen of patients with infertility. Turk J Med Sci. 2015;45:443-8 pubmed
    ..05). In the present study, we found significant correlations between concentrations of Zn, Ca, Al, Cu, Mg, Se, and Sr and semen parameters in various body fluids. ..
  14. Stasiak K, Glogowski J, Demianowicz W, Kowalski R, Nowak Tkaczyk A, Janicki B. Use of biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus). Pol J Vet Sci. 2014;17:427-32 pubmed
    ..In addition, several significant relationships were found between some indicators of semen quality and plasma and/or supernatant enzyme activity. ..
  15. Yan L, Liu J, Wu S, Zhang S, Ji G, Gu A. Seminal superoxide dismutase activity and its relationship with semen quality and SOD gene polymorphism. J Assist Reprod Genet. 2014;31:549-54 pubmed
    ..8?±?93.3 and 332.2?±?94.9, respectively, P?=?0.001). Seminal SOD activity and other factors influencing SOD activity play a role in determining sperm fertilization potential and male infertility. ..