skin test end point titration


Summary: A quantitative form of intradermal testing for the relative allergenicity of a substance. It is used to determine the amount of an allergen that will be tolerated in ALLERGEN IMMUNOTHERAPY.

Top Publications

  1. Chambers D, Cook P, Nishioka G, Erhart P. Comparison of mRAST and CAP with skin end point titration for Alternaria tenuis and dermatophagoides pteronyssinus. Otolaryngol Head Neck Surg. 1997;117:471-4 pubmed
    ..These results were then compared with the results of patients who also underwent intradermal skin testing based on end point titration techniques. ..
  2. Simons J, Rubinstein E, Kogut V, Melfi P, Ferguson B. Comparison of Multi-Test II skin prick testing to intradermal dilutional testing. Otolaryngol Head Neck Surg. 2004;130:536-44 pubmed
    ..The clinical significance of positive IDT results in the presence of negative Multi-Test II results is not known. ..
  3. Peltier J, Ryan M. Comparison of intradermal dilutional testing with the Multi-Test II applicator in testing for mold allergy. Otolaryngol Head Neck Surg. 2006;134:240-4 pubmed
    ..MQT is nearly as effective as formal IDT in determining endpoint. MQT appears to be a safe method for determining starting doses for immunotherapy with fungal allergens. ..
  4. Peltier J, Ryan M. Comparison of intradermal dilutional testing, skin prick testing, and modified quantitative testing for common allergens. Otolaryngol Head Neck Surg. 2007;137:246-9 pubmed
    ..MQT is nearly as effective as formal IDT in determining endpoint. Modified quantitative testing appears to be a safe alternative to IDT for determining starting doses for immunotherapy. ..
  5. Meglio P, Giampietro P, Gianni S, Galli E. Oral desensitization in children with immunoglobulin E-mediated cow's milk allergy--follow-up at 4 yr and 8 months. Pediatr Allergy Immunol. 2008;19:412-9 pubmed publisher
    ..Oral CM desensitization seems to be a promising method to treat CM food allergy. This protocol is time-consuming but offers the advantage that it can be performed at home. This methodology must only be used by trained staff...
  6. King H. Skin endpoint titration. Still the standard?. Otolaryngol Clin North Am. 1992;25:13-25 pubmed
    ..Other forms of skin testing, simpler to perform, are rapidly becoming more uniform and quantitative. Laboratory tests provide more specific information and improved convenience. All occupy important positions in allergy diagnosis. ..
  7. Mabry R. Blending skin endpoint titration and in vitro methods in clinical practice. Otolaryngol Clin North Am. 1992;25:61-70 pubmed
  8. Grammer L, Shaughnessy M, Holmquist L, Pruzansky J, Patterson R. Polymerization and fractionation of house dust mite allergen. Allergy Proc. 1993;14:195-9 pubmed
    ..In summary, we have prepared PDM which has the desired characteristics of reduced allergenicity, retained immunogenicity, antigenic completeness, and absence of new antigenic determinants. ..
  9. Lokesh G, Rachamallu A, Kumar G, Natarajan A. High-throughput fluorescence polarization assay to identify small molecule inhibitors of BRCT domains of breast cancer gene 1. Anal Biochem. 2006;352:135-41 pubmed
    ..9+/-1.9. The assay tolerates 20% dimethyl sulfoxide, which enables screening poorly soluble compounds. Under optimized conditions, a Z' factor of 0.87 was achieved in a 384-well format for high-throughput screening. ..

More Information


  1. Schiff M, Boris M, Weindorf S. Low doses of animal antigen attenuates the late asthmatic reaction. Otolaryngol Head Neck Surg. 1992;107:91-4 pubmed
    ..05). In this model of antigen-induced asthma, injection of low doses of allergen immediately preceding bronchoprovocation blunted the immediate and delayed asthmatic reaction. ..
  2. Santucci B, Valenzano C, De Rocco M, Cristaudo A. Platinum in the environment: frequency of reactions to platinum-group elements in patients with dermatitis and urticaria. Contact Dermatitis. 2000;43:333-8 pubmed
    ..However, if the average level of environmental platinum exposure approaches those existing in industrial settings in the future, we are going to observe more frequent health effects. ..
  3. Mori F, Pucci N, Rossi M, de Martino M, Azzari C, Novembre E. Oral desensitization to milk: how to choose the starting dose!. Pediatr Allergy Immunol. 2010;21:e450-3 pubmed publisher
  4. Dey S, Mohan C, Ramadass P, Nachimuthu K. Diagnosis of leptospirosis by recombinant antigen based single serum dilution ELISA. Indian J Med Res. 2008;128:172-7 pubmed
  5. Krouse J, Mabry R. Skin testing for inhalant allergy 2003: current strategies. Otolaryngol Head Neck Surg. 2003;129:S33-49 pubmed
  6. Krouse J, Krouse H. Efficacy of immunotherapy based on skin end-point titration. Otolaryngol Head Neck Surg. 2000;123:183-7 pubmed
    ..SET-based immunotherapy reduces self-rated disability over time. In addition, SET-based immunotherapy allows decrease in nasal reactivity and normalizes the endoscopic appearance of the nose. ..
  7. Färm G. Repeated open application tests (ROAT) in patients allergic to colophony--evaluated visually and with bioengineering techniques. Acta Derm Venereol. 1998;78:130-5 pubmed
    ..There was great variation in the reactivity in the ROATs. Objective measures for evaluating the ROAT reactions gave no further information than visual assessment. ..
  8. Corey J, Mamikoglu B, Akbar I, Houser S, Gungor A. ImmunoCAP and HY*TEC enzyme immunoassays in the detection of allergen-specific IgE compared with serial skin end-point titration by receiver operating characteristic analysis. Otolaryngol Head Neck Surg. 2000;122:64-70 pubmed
    ..The HY*TEC assay did not give acceptable results for A fumigatus. The overall assay characteristics were good to excellent for each system tested. ..
  9. Fadal R. Experience with RAST-based immunotherapy. Otolaryngol Clin North Am. 1992;25:43-60 pubmed
    ..The use of RAST as a method of allergen standardization only reinforces its value in patient management. ..
  10. Gordon B. Allergy skin tests and immunotherapy: comparison of methods in common use. Ear Nose Throat J. 1990;69:47-54, 57-62 pubmed
    ..Concepts of low-dose, symptom-relieving dose, and maximally-tolerated dose immunotherapy are described, and common factors in all allergy techniques are stressed. ..
  11. Franzese C, Burkhalter N. The patient with allergies. Med Clin North Am. 2010;94:891-902 pubmed publisher
    ..Key history and physical examination findings help to distinguish allergic rhinitis from other forms of chronic rhinosinusitis. Diagnostic testing may not be necessary unless immunotherapy is contemplated. ..
  12. Kemp A, Chiang W, Gerez I, Goh A, Liew W, Shek L, et al. Childhood food allergy: a Singaporean perspective. Ann Acad Med Singapore. 2010;39:404-11 pubmed
    ..This review is aimed at providing the clinician with a Singaporean perspective on the clinical approach and management of these disorders. ..
  13. Bernstein D, Bernstein I, Gaines W, Stauder T, Wilson E. Characterization of skin prick testing responses for detecting sensitization to detergent enzymes at extreme dilutions: inability of the RAST to detect lightly sensitized individuals. J Allergy Clin Immunol. 1994;94:498-507 pubmed
    ..The excellent correlations of both wheal diameter and area with antigen concentrations were attributed to the very small changes observed between test concentrations. ..
  14. Anon J. Introduction to in vivo allergy testing. Otolaryngol Head Neck Surg. 1993;109:593-600 pubmed
    ..It is concluded that each method of in vivo skin testing is associated with both advantages and disadvantages and that the most important factors are sensitivity and reproducibility. ..
  15. Gutman M, Torres A, Keen K, Houser S. Prevalence of allergy in patients with chronic rhinosinusitis. Otolaryngol Head Neck Surg. 2004;130:545-52 pubmed
    ..Perennial allergy has a statistically significant association with chronic and recurrent acute rhinosinusitis. The diagnosis and management of perennial allergies may be beneficial when treating chronic sinus disease. ..
  16. Stroud R, Calhoun K, Wright S, Kennedy K. Prevalence of hypersensitivity to specific fungal allergens as determined by intradermal dilutional testing. Otolaryngol Head Neck Surg. 2001;125:491-4 pubmed
    ..Fungal allergens were as likely as nonfungal to elicit reactivity. These findings suggest a role for fungal hypersensitivity in chronic rhinitis and chronic rhinosinusitis. ..
  17. Karrow N, Leffel E, Guo T, Zhang L, McCay J, Germolec D, et al. Dermal exposure to cinnamaldehyde alters lymphocyte subpopulations, number of interferon-gamma-producing cells, and expression of B7 costimulatory molecules and cytokine messenger RNAs in auricular lymph nodes of B6C3F1 mice. Am J Contact Dermat. 2001;12:6-17 pubmed
    ..However, they may help identify compounds inappropriately classified as sensitizers or nonsensitizers by the LLNA and MEST. ..
  18. Seshul M, Pillsbury H, Eby T. Use of intradermal dilutional testing and skin prick testing: clinical relevance and cost efficiency. Laryngoscope. 2006;116:1530-8 pubmed
    ..Blended allergy testing techniques that include IDT in their protocol are comparable in cost with commonly used allergy testing protocols. ..
  19. Tandy J, Mabry R, Mabry C. Correlation of modified radioallergosorbent test scores and skin test results. Otolaryngol Head Neck Surg. 1996;115:42-5 pubmed
    ..The relationship between radioallergosorbent test and skin test results will be critically analyzed. ..
  20. Laidlaw T, Booth R, Large R. Reduction in skin reactions to histamine after a hypnotic procedure. Psychosom Med. 1996;58:242-8 pubmed
    ..This study has shown highly significant results in reducing skin sensitivity to histamine using a cognitive-hypnotic technique, which indicates some promise for extending this work into the clinical area. ..
  21. Derebery M, Valenzuela S. Ménière's syndrome and allergy. Otolaryngol Clin North Am. 1992;25:213-24 pubmed
    ..By proper recognition and treatment of the underlying allergic factors affecting Ménière's disease, significant clinical improvement may be obtained even in longstanding or refractory cases. ..