laser capture microdissection


Summary: Techniques using a laser to cut away and harvest a specific cell or cluster of cells from a tissue section while viewing it under the microscope.

Top Publications

  1. Bandyopadhyay U, Fenton W, Horwich A, Nagy M. Production of RNA for transcriptomic analysis from mouse spinal cord motor neuron cell bodies by laser capture microdissection. J Vis Exp. 2014;:e51168 pubmed publisher
    ..b>Laser capture microdissection (LMD) has been developed to address this problem...
  2. Neuwirt H, Perco P, Kainz A, Mühlberger I, Leierer J, Braniff S, et al. A 3-biomarker-panel predicts renal outcome in patients with proteinuric renal diseases. BMC Med Genomics. 2014;7:75 pubmed publisher
    ..0115). We identified and validated a panel of three genes in kidney biopsies which predicted serum creatinine at follow-up and therefore might serve as biomarkers for kidney disease progression. ..
  3. Kummari E, Guo Ross S, Eells J. Laser capture microdissection--a demonstration of the isolation of individual dopamine neurons and the entire ventral tegmental area. J Vis Exp. 2015;:e52336 pubmed publisher
    b>Laser capture microdissection (LCM) is used to isolate a concentrated population of individual cells or precise anatomical regions of tissue from tissue sections on a microscope slide...
  4. Chishiki M, Takagi K, Sato A, Miki Y, Yamamoto Y, Ebata A, et al. Cytochrome c1 in ductal carcinoma in situ of breast associated with proliferation and comedo necrosis. Cancer Sci. 2017;108:1510-1519 pubmed publisher
    ..Considering that the center of DCIS is poorly oxygenated, these results indicate that CYC1 plays important roles in cell proliferation and comedo necrosis through the elevated oxidative phosphorylation activity in human DCIS. ..
  5. Zhou J, Chen Q, Lanske B, Fleming B, Terek R, Wei X, et al. Disrupting the Indian hedgehog signaling pathway in vivo attenuates surgically induced osteoarthritis progression in Col2a1-CreERT2; Ihhfl/fl mice. Arthritis Res Ther. 2014;16:R11 pubmed publisher
    ..Thus, our findings suggest the potential to develop new therapeutic strategies that can prevent and treat OA by inhibiting Ihh signaling in chondrocytes. ..
  6. Denisov E, Skryabin N, Vasilyev S, Gerashchenko T, Lebedev I, Zavyalova M, et al. Relationship between morphological and cytogenetic heterogeneity in invasive micropapillary carcinoma of the breast: a report of one case. J Clin Pathol. 2015;68:758-62 pubmed publisher
  7. Chinetti Gbaguidi G, Daoudi M, Rosa M, Vinod M, Louvet L, Copin C, et al. Human Alternative Macrophages Populate Calcified Areas of Atherosclerotic Lesions and Display Impaired RANKL-Induced Osteoclastic Bone Resorption Activity. Circ Res. 2017;121:19-30 pubmed publisher
    ..These results indicate that macrophages surrounding calcium deposits in human atherosclerotic plaques are phenotypically defective being unable to resorb calcification. ..
  8. Nyalwidhe J, Grzesik W, Burch T, Semeraro M, Waseem T, Gerling I, et al. Comparative quantitative proteomic analysis of disease stratified laser captured microdissected human islets identifies proteins and pathways potentially related to type 1 diabetes. PLoS ONE. 2017;12:e0183908 pubmed publisher
    ..mass accuracy label free quantitative mass spectrometry analysis was applied to islets isolated by laser capture microdissection from disease stratified human pancreata from the Network for Pancreatic Organ Donors with Diabetes (..
  9. Garrison P, Yue S, Hanson J, Baron J, Lui J. Spatial regulation of bone morphogenetic proteins (BMPs) in postnatal articular and growth plate cartilage. PLoS ONE. 2017;12:e0176752 pubmed publisher
    ..To investigate the presence of a similar expression gradient in articular cartilage, we used laser capture microdissection (LCM) to separate murine growth plate and articular cartilage from the proximal tibia into their six ..

More Information


  1. Fernández Nestosa M, Guimera N, Sanchez D, Cañete Portillo S, Velazquez E, Jenkins D, et al. Human Papillomavirus (HPV) Genotypes in Condylomas, Intraepithelial Neoplasia, and Invasive Carcinoma of the Penis Using Laser Capture Microdissection (LCM)-PCR: A Study of 191 Lesions in 43 Patients. Am J Surg Pathol. 2017;41:820-832 pubmed publisher
    b>Laser capture microdissection-polymerase chain reaction (LCM-PCR) supported by p16 was used for the first time to demonstrate human papillomavirus (HPV) DNA in histologically specific penile lesions, which were as follows: squamous ..
  2. Hara M, Kobayakawa K, Ohkawa Y, Kumamaru H, Yokota K, Saito T, et al. Interaction of reactive astrocytes with type I collagen induces astrocytic scar formation through the integrin-N-cadherin pathway after spinal cord injury. Nat Med. 2017;23:818-828 pubmed publisher
    ..Our findings reveal environmental cues regulating astrocytic fate decisions, thereby providing a potential therapeutic target for CNS injury. ..
  3. Kwon H, Jia S, Lan Y, Liu H, Jiang R. Activin and Bmp4 Signaling Converge on Wnt Activation during Odontogenesis. J Dent Res. 2017;96:1145-1152 pubmed publisher
  4. Boone D, Leek J, Falduto M, Torres K, Sell S, Parsley M, et al. Effects of AAV-mediated knockdown of nNOS and GPx-1 gene expression in rat hippocampus after traumatic brain injury. PLoS ONE. 2017;12:e0185943 pubmed publisher
    ..Since nNOS knockdown only modestly ameliorated TBI-induced working memory deficits, despite widespread genomic changes, manipulating expression levels of single genes may not be sufficient to alter functional outcome after TBI. ..
  5. Hogekamp C, Küster H. A roadmap of cell-type specific gene expression during sequential stages of the arbuscular mycorrhiza symbiosis. BMC Genomics. 2013;14:306 pubmed publisher
  6. Jan S, Vormer T, Jongejan A, Röling M, Silber S, de Rooij D, et al. Unraveling transcriptome dynamics in human spermatogenesis. Development. 2017;144:3659-3673 pubmed publisher
    ..These established transcriptomes provide a reference catalog for further detailed studies on human spermatogenesis and spermatogenic failure. ..
  7. Ibrahim I, Serrano M, Ruest L, Svoboda K. Biglycan and Decorin Expression and Distribution in Palatal Adhesion. J Dent Res. 2017;96:1445-1450 pubmed publisher
    ..b>Laser capture microdissection was used to collect selected palatal epithelial cells from embryonic mouse embryos at various palate ..
  8. Loveridge C, Mui E, Patel R, Tan E, Ahmad I, Welsh M, et al. Increased T-cell Infiltration Elicited by Erk5 Deletion in a Pten-Deficient Mouse Model of Prostate Carcinogenesis. Cancer Res. 2017;77:3158-3168 pubmed publisher
    ..i>Cancer Res; 77(12); 3158-68. ©2017 AACR. ..
  9. Aydin S, Ambroise J, Cosyns J, Gala J. TP53 mutations in p53-negative dysplastic urothelial cells from Belgian AAN patients: New evidence for aristolochic acid-induced molecular pathogenesis and carcinogenesis. Mutat Res. 2017;818:17-26 pubmed publisher
    ..Consequenlty, current results bring additional and valuable evidence unravelling the molecular pathogenesis of AA-induced carcinogenesis and the origin of related high-grade UCs. ..
  10. Hanley M, Hahn M, Li A, Wu X, Lin J, Wang J, et al. Genome-wide DNA methylation profiling reveals cancer-associated changes within early colonic neoplasia. Oncogene. 2017;36:5035-5044 pubmed publisher
  11. Cubi R, Vembar S, Biton A, Franetich J, Bordessoulles M, Sossau D, et al. Laser capture microdissection enables transcriptomic analysis of dividing and quiescent liver stages of Plasmodium relapsing species. Cell Microbiol. 2017;19: pubmed publisher
  12. Bodelon C, Vinokurova S, Sampson J, den Boon J, Walker J, Horswill M, et al. Chromosomal copy number alterations and HPV integration in cervical precancer and invasive cancer. Carcinogenesis. 2016;37:188-196 pubmed publisher