cell fusion


Summary: Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.

Top Publications

  1. Bu Y, Cao D. The origin of cancer stem cells. Front Biosci (Schol Ed). 2012;4:819-30 pubmed
    ..Up to date, several hypotheses have been described, and cell fusion and horizontal gene transfer, which may occur during development and tissue repair process, are considered as ..
  2. Richard J, Leikina E, Langen R, Henne W, Popova M, Balla T, et al. Intracellular curvature-generating proteins in cell-to-cell fusion. Biochem J. 2011;440:185-93 pubmed publisher
    Cell-to-cell fusion plays an important role in normal physiology and in different pathological conditions...
  3. Wakimoto H, Shimodo M, Satoh Y, Kitagawa Y, Takeuchi K, Gotoh B, et al. F-actin modulates measles virus cell-cell fusion and assembly by altering the interaction between the matrix protein and the cytoplasmic tail of hemagglutinin. J Virol. 2013;87:1974-84 pubmed publisher
    ..for the sharply declined association with F-actin, the mutant M (M-F50P) protein strongly inhibited MV cell-cell fusion and promoted the uptake of the H protein into virus particles...
  4. Kuo S, Chen Y, Wang Y, Kuo M, Jinn T, Chen W, et al. Cell-based analysis of Chikungunya virus membrane fusion using baculovirus-expression vectors. J Virol Methods. 2011;175:206-15 pubmed publisher
    ..The structural proteins of Chikungunya virus that is required for cell fusion was determined with various recombinant baculoviruses bearing different lengths of the viral structural protein ..
  5. Doherty J, Lenhart K, Cameron M, Mack C, Conlon F, Taylor J. Skeletal muscle differentiation and fusion are regulated by the BAR-containing Rho-GTPase-activating protein (Rho-GAP), GRAF1. J Biol Chem. 2011;286:25903-21 pubmed publisher
    ..Our results are the first to identify a GTPase-activating protein that regulates muscle maturation and to highlight the functional importance of BAR domains in myotube formation...
  6. Fu C, Iyer P, Herkal A, Abdullah J, Stout A, Free S. Identification and characterization of genes required for cell-to-cell fusion in Neurospora crassa. Eukaryot Cell. 2011;10:1100-9 pubmed publisher
    A screening procedure was used to identify cell fusion (hyphal anastomosis) mutants in the Neurospora crassa single gene deletion library...
  7. Goldenberg D. Horizontal transmission of malignancy by cell-cell fusion. Expert Opin Biol Ther. 2012;12 Suppl 1:S133-9 pubmed publisher
    ..Cell-cell fusion in vivo is one of several mechanisms by which genetic information can be transmitted from tumor to host cells, ..
  8. Foshay K, Looney T, Chari S, Mao F, Lee J, Zhang L, et al. Embryonic stem cells induce pluripotency in somatic cell fusion through biphasic reprogramming. Mol Cell. 2012;46:159-70 pubmed publisher
    It is a long-held paradigm that cell fusion reprograms gene expression but the extent of reprogramming and whether it is affected by the cell types employed remain unknown...
  9. Abmayr S, Pavlath G. Myoblast fusion: lessons from flies and mice. Development. 2012;139:641-56 pubmed publisher
    ..enhance our understanding of myogenesis, but also contribute to our perspectives on membrane fusion and cell-cell fusion in a wide array of model organisms and experimental systems...

Scientific Experts

More Information


  1. Terashima T, Kojima H, Chan L. Bone marrow expression of poly(ADP-ribose) polymerase underlies diabetic neuropathy via hematopoietic-neuronal cell fusion. FASEB J. 2012;26:295-308 pubmed publisher
    ..These complementary in vivo and in vitro experiments indicate that BMDC-PARP expression promotes diabetic neuropathy via BMDC-neuron fusion. ..
  2. Oliver S, Brady J, Sommer M, Reichelt M, Sung P, Blau H, et al. An immunoreceptor tyrosine-based inhibition motif in varicella-zoster virus glycoprotein B regulates cell fusion and skin pathogenesis. Proc Natl Acad Sci U S A. 2013;110:1911-6 pubmed publisher
    ..functions of the conserved glycoproteins gB and gH-gL have been delineated, but their role in regulating cell-cell fusion is poorly understood...
  3. Tsubouchi T, Soza Ried J, Brown K, Piccolo F, Cantone I, Landeira D, et al. DNA synthesis is required for reprogramming mediated by stem cell fusion. Cell. 2013;152:873-83 pubmed publisher
    ..stem cells (ESCs) can instruct the conversion of differentiated cells toward pluripotency following cell-to-cell fusion by a mechanism that is rapid but poorly understood...
  4. Song Y, Pinkernell K, Alt E. Stem cell induced cardiac regeneration: fusion/mitochondrial exchange and/or transdifferentiation?. Cell Cycle. 2011;10:2281-6 pubmed
  5. Charrin S, Latil M, Soave S, Polesskaya A, Chretien F, Boucheix C, et al. Normal muscle regeneration requires tight control of muscle cell fusion by tetraspanins CD9 and CD81. Nat Commun. 2013;4:1674 pubmed publisher
    ..Our study unveils a mechanism preventing inappropriate fusion of myotubes that has an important role in the restitution of normal muscle architecture during muscle regeneration. ..
  6. Simmons G, Bertram S, Glowacka I, Steffen I, Chaipan C, Agudelo J, et al. Different host cell proteases activate the SARS-coronavirus spike-protein for cell-cell and virus-cell fusion. Virology. 2011;413:265-74 pubmed publisher
    ..We found that R667 was dispensable for SARS-S-driven virus-cell fusion and for SARS-S-activation by trypsin and cathepsin L in a virus-virus fusion assay...
  7. Hubbard S, Choudhary S, Maus E, Shukla D, Swenson S, Markland F, et al. Contortrostatin, a homodimeric disintegrin isolated from snake venom inhibits herpes simplex virus entry and cell fusion. Antivir Ther. 2012;17:1319-26 pubmed publisher
    ..In this study, we evaluated contortrostatin (CN), an Arg-Gly-Asp motif containing disintegrin isolated from snake venom, as a novel therapeutic agent with ability to block HSV entry and related membrane fusion...
  8. Lazova R, Chakraborty A, Pawelek J. Leukocyte-cancer cell fusion: initiator of the warburg effect in malignancy?. Adv Exp Med Biol. 2011;714:151-72 pubmed publisher
    ..Here we review evidence that these phenomena can be well-explained by macrophage-cancer cell fusion, as evidenced by studies of experimental macrophage-melanoma hybrids generated in vitro and spontaneous host-..
  9. Miyamoto H, Katsuyama E, Miyauchi Y, Hoshi H, Miyamoto K, Sato Y, et al. An essential role for STAT6-STAT1 protein signaling in promoting macrophage cell-cell fusion. J Biol Chem. 2012;287:32479-84 pubmed
    ..Macrophage lineage cells such as osteoclasts and foreign body giant cells (FBGCs) form multinuclear cells by cell-cell fusion of mononuclear cells...
  10. Rappa G, Mercapide J, Lorico A. Spontaneous formation of tumorigenic hybrids between breast cancer and multipotent stromal cells is a source of tumor heterogeneity. Am J Pathol. 2012;180:2504-15 pubmed publisher
    ..Our findings reconcile the fusion theory of cancer progression with the common observation that breast cancer metastases are generally aneuploid, but not tetraploid, and are histopathologically similar to the primary neoplasm...
  11. Carloni V, Mazzocca A, Mello T, Galli A, Capaccioli S. Cell fusion promotes chemoresistance in metastatic colon carcinoma. Oncogene. 2013;32:2649-60 pubmed publisher
    ..b>Cell fusion represents an efficient means of rapid phenotypic evolution that make cells with new properties at a rate ..
  12. Gruenbaum Cohen Y, Harel I, Umansky K, Tzahor E, Snapper S, Shilo B, et al. The actin regulator N-WASp is required for muscle-cell fusion in mice. Proc Natl Acad Sci U S A. 2012;109:11211-6 pubmed publisher
    A fundamental aspect of skeletal myogenesis involves extensive rounds of cell fusion, in which individual myoblasts are incorporated into growing muscle fibers...
  13. Holm C, Jensen S, Jakobsen M, Cheshenko N, Horan K, Moeller H, et al. Virus-cell fusion as a trigger of innate immunity dependent on the adaptor STING. Nat Immunol. 2012;13:737-43 pubmed publisher
    ..Virus-cell fusion specifically stimulated a type I interferon response with expression of interferon-stimulated genes, in vivo ..
  14. Alvarez Dolado M, Martinez Losa M. Cell fusion and tissue regeneration. Adv Exp Med Biol. 2011;713:161-75 pubmed publisher
    b>Cell fusion is a natural process implicated in normal development, immune response, tissue formation, and with a prominent role in stem cell plasticity...
  15. Fan Q, Amen M, Harden M, Severini A, Griffiths A, Longnecker R. Herpes B virus utilizes human nectin-1 but not HVEM or PILR? for cell-cell fusion and virus entry. J Virol. 2012;86:4468-76 pubmed publisher
    ..Cell-cell fusion assays indicated that nectin-1, an HSV-1 gD receptor, mediated fusion of cells expressing glycoproteins from ..
  16. Pérez Vargas J, Krey T, Valansi C, Avinoam O, Haouz A, Jamin M, et al. Structural basis of eukaryotic cell-cell fusion. Cell. 2014;157:407-419 pubmed publisher
    Cell-cell fusion proteins are essential in development. Here we show that the C. elegans cell-cell fusion protein EFF-1 is structurally homologous to viral class II fusion proteins. The 2...
  17. Koido S, Homma S, Okamoto M, Namiki Y, Takakura K, Takahara A, et al. Combined TLR2/4-activated dendritic/tumor cell fusions induce augmented cytotoxic T lymphocytes. PLoS ONE. 2013;8:e59280 pubmed publisher
    ..Incorporating combined TLRs-activation and TGF-?1-blockade of DC/tumor may enhance the effectiveness of DC/tumor-based cancer vaccines and have the potential applicability to the field of adoptive immunotherapy. ..
  18. Chuprin A, Gal H, Biron Shental T, Biran A, Amiel A, Rozenblatt S, et al. Cell fusion induced by ERVWE1 or measles virus causes cellular senescence. Genes Dev. 2013;27:2356-66 pubmed publisher
    ..During embryonic development, ERVWE1-mediated cell fusion results in formation of the syncytiotrophoblast, which serves as the maternal/fetal interface at the placenta...
  19. Oikawa T, Oyama M, Kozuka Hata H, Uehara S, Udagawa N, Saya H, et al. Tks5-dependent formation of circumferential podosomes/invadopodia mediates cell-cell fusion. J Cell Biol. 2012;197:553-68 pubmed publisher
    ..Our results thus reveal an unexpected link between circumferential podosome/invadopodium formation and cell-cell fusion in and beyond osteoclasts.
  20. Roy N, Chan J, Lambelé M, Thali M. Clustering and mobility of HIV-1 Env at viral assembly sites predict its propensity to induce cell-cell fusion. J Virol. 2013;87:7516-25 pubmed publisher
  21. Koido S, Gong J. Characterization of structure and direct antigen presentation by dendritic/tumor-fused cells as cancer vaccines. Anticancer Res. 2013;33:347-54 pubmed
    ..DC-derived molecules and TAAs are presumably synthesized at separate sites of fused cells, to converge and complex with each other. ..
  22. Krajcovic M, Overholtzer M. Mechanisms of ploidy increase in human cancers: a new role for cell cannibalism. Cancer Res. 2012;72:1596-601 pubmed publisher
    ..Polyploid cells can originate from cell fusion, endoreplication, and cytokinesis failure...
  23. Metz S, Geertsema C, Martina B, Andrade P, Heldens J, van Oers M, et al. Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells. Virol J. 2011;8:353 pubmed publisher
    ..The ability of purified, secreted CHIKV-E2 to induce neutralizing antibodies in rabbits underscores the potential use of E2 in a subunit vaccine to prevent CHIKV infections. ..
  24. Serov O, Matveeva N, Khabarova A. Reprogramming mediated by cell fusion technology. Int Rev Cell Mol Biol. 2011;291:155-90 pubmed publisher
    ..The first step is realized without cell division, whereas the second requires cell proliferation. Reprogramming in hybrid cells is rapid and complete. Thus, cell fusion is a powerful tool for reprogramming.
  25. Koido S, Homma S, Takahara A, Namiki Y, Komita H, Nagasaki E, et al. Immunologic monitoring of cellular responses by dendritic/tumor cell fusion vaccines. J Biomed Biotechnol. 2011;2011:910836 pubmed publisher
    ..In this review, we will discuss immunologic monitoring of cellular responses for the evaluation of cancer vaccines including fusions of DC and whole tumor cell. ..
  26. Yang W, Li S, Weisel R, Liu S, Li R. Cell fusion contributes to the rescue of apoptotic cardiomyocytes by bone marrow cells. J Cell Mol Med. 2012;16:3085-95 pubmed publisher
    ..We therefore hypothesized that cell fusion may be a major mechanism by which BMCs rescue cardiomyocytes from apoptosis...
  27. Berndt B, Zanker K, Dittmar T. Cell fusion is a potent inducer of aneuploidy and drug resistance in tumor cell/ normal cell hybrids. Crit Rev Oncog. 2013;18:97-113 pubmed
    The biological phenomenon of cell fusion is involved in several physiological (fertilization, tissue regeneration) and pathophysiological (viral infection, cancer) processes...
  28. Fischer I, Weber M, Kuhn C, Fitzgerald J, Schulze S, Friese K, et al. Is galectin-1 a trigger for trophoblast cell fusion?: the MAP-kinase pathway and syncytium formation in trophoblast tumour cells BeWo. Mol Hum Reprod. 2011;17:747-57 pubmed publisher
    ..Furthermore, we demonstrated that gal-1 induces the activation of Elk1, a transcription factor that is activated by MAPK pathways. ..
  29. Chen Q, Huang S, Chen J, Zhang S, Chen Z. NA proteins of influenza A viruses H1N1/2009, H5N1, and H9N2 show differential effects on infection initiation, virus release, and cell-cell fusion. PLoS ONE. 2013;8:e54334 pubmed publisher
    ..In addition, in MDCK cells, rPR8-H5N1NA and rPR8-H9N2NA infected a higher percentage of cells, and induced cell-cell fusion faster and more extensively than PR8-wt and rPR8-H1N1NA did in the early phase of infection...
  30. Wei Y, Feng K, Yao X, Cai H, Li J, Mirza A, et al. Localization of a region in the fusion protein of avian metapneumovirus that modulates cell-cell fusion. J Virol. 2012;86:11800-14 pubmed publisher
    ..Furthermore, there are notable differences in cell-cell fusion among aMPV subtypes. Trypsin was required for cell-cell fusion induced by subtype B but not subtypes A and C...
  31. Soza Ried J, Fisher A. Reprogramming somatic cells towards pluripotency by cellular fusion. Curr Opin Genet Dev. 2012;22:459-65 pubmed publisher
    ..In this review we summarise some recent advances using cell fusion-based studies to better understand the basis of pluripotency and the epigenetic mechanisms that promote cell ..
  32. Matsuura K, Jigami T, Taniue K, Morishita Y, Adachi S, Senda T, et al. Identification of a link between Wnt/?-catenin signalling and the cell fusion pathway. Nat Commun. 2011;2:548 pubmed publisher
    b>Cell fusion has a critical role in various developmental processes, immune response, tissue homeostasis and regeneration, and possibly, in cancer...
  33. Dittmar T, Nagler C, Niggemann B, Zanker K. The dark side of stem cells: triggering cancer progression by cell fusion. Curr Mol Med. 2013;13:735-50 pubmed
    The phenomenon of cell fusion plays a crucial role in a plethora of physiological processes, including fertilization, wound healing, and tissue regeneration...
  34. Atanasiu D, Saw W, Gallagher J, Hannah B, Matsuda Z, Whitbeck J, et al. Dual split protein-based fusion assay reveals that mutations to herpes simplex virus (HSV) glycoprotein gB alter the kinetics of cell-cell fusion induced by HSV entry glycoproteins. J Virol. 2013;87:11332-45 pubmed publisher
    Herpes simplex virus (HSV) entry and cell-cell fusion require glycoproteins gD, gH/gL, and gB. We propose that receptor-activated changes to gD cause it to activate gH/gL, which then triggers gB into an active form...
  35. Sumer H, Nicholls C, Liu J, Tat P, Liu J, Verma P. Comparison of reprogramming ability of mouse ES and iPS cells measured by somatic cell fusion. Int J Dev Biol. 2010;54:1723-8 pubmed publisher
    ..stem (iPS) cells, like embryonic stem (ES) cells, have been shown to be able to reprogram somatic cells by cell fusion. In this study we compare the differences and similarities between ES and iPS cells measured by somatic cell ..
  36. Okayasu M, Nakayachi M, Hayashida C, Ito J, Kaneda T, Masuhara M, et al. Low-density lipoprotein receptor deficiency causes impaired osteoclastogenesis and increased bone mass in mice because of defect in osteoclastic cell-cell fusion. J Biol Chem. 2012;287:19229-41 pubmed publisher
    ..cells formed in culture were smaller and contained fewer nuclei than wild-type cells, implying impaired cell-cell fusion. Despite these findings, RANK signaling, including the activation of Erk and Akt, was normal in LDLR(-/-) ..
  37. Haralalka S, Shelton C, Cartwright H, Katzfey E, Janzen E, Abmayr S. Asymmetric Mbc, active Rac1 and F-actin foci in the fusion-competent myoblasts during myoblast fusion in Drosophila. Development. 2011;138:1551-62 pubmed publisher
    ..These data are consistent with models promoting actin polymerization as the driving force for myoblast fusion. ..
  38. Shilagardi K, Li S, Luo F, Marikar F, Duan R, Jin P, et al. Actin-propelled invasive membrane protrusions promote fusogenic protein engagement during cell-cell fusion. Science. 2013;340:359-63 pubmed publisher
    Cell-cell fusion is critical for the conception, development, and physiology of multicellular organisms...
  39. Silvestris F, Ciavarella S, Strippoli S, Dammacco F. Cell fusion and hyperactive osteoclastogenesis in multiple myeloma. Adv Exp Med Biol. 2011;714:113-28 pubmed publisher
  40. Mähs A, Ischebeck T, Heilig Y, Stenzel I, Hempel F, Seiler S, et al. The essential phosphoinositide kinase MSS-4 is required for polar hyphal morphogenesis, localizing to sites of growth and cell fusion in Neurospora crassa. PLoS ONE. 2012;7:e51454 pubmed publisher
    ..The data presented for N. crassa MSS-4 and its roles in hyphal growth are discussed with a comparative perspective on PI-control of polar tip growth in different organismic kingdoms...
  41. Wang R, Sun X, Wang C, Hu P, Chu C, Liu S, et al. Spontaneous cancer-stromal cell fusion as a mechanism of prostate cancer androgen-independent progression. PLoS ONE. 2012;7:e42653 pubmed publisher
    ..Cancer-stromal fusion thus lays a foundation for an incessant co-evolution between cancer and the cancer-associated stromal cells in the tumor microenvironment...
  42. Kuetemeyer K, Lucas Hahn A, Petersen B, Niemann H, Heisterkamp A. Femtosecond laser-induced fusion of nonadherent cells and two-cell porcine embryos. J Biomed Opt. 2011;16:088001 pubmed publisher
    b>Cell fusion is a fundamental biological process that can be artificially induced by different methods...
  43. Jeong J, Conboy I. Phosphatidylserine directly and positively regulates fusion of myoblasts into myotubes. Biochem Biophys Res Commun. 2011;414:9-13 pubmed publisher
    ..the cell-cell contact points, suggesting the importance of membrane ruffling and PS exposure for the myogenic cell fusion. Confirming this conclusion, experimentally constructed PS, but not PC liposomes dramatically enhance the ..
  44. Gou S, Liu T, Li X, Cui J, Wan C, Wang C. Pancreatic ductal cells acquire mesenchymal characteristics through cell fusion with bone marrow-derived mesenchymal stem cells and SIRT1 attenuates the apoptosis of hybrid cells. Cells Tissues Organs. 2012;196:129-36 pubmed publisher
    Bone marrow-derived mesenchymal stem cells (bMSCs) contribute to tissue repair and regeneration. Cell fusion between somatic cells and bMSCs to form hybrid cells may have an important role in tissue repair through the subsequent ..
  45. Quintana Bustamante O, Grueso E, García Escudero R, Arza E, Alvarez Barrientos A, Fabregat I, et al. Cell fusion reprogramming leads to a specific hepatic expression pattern during mouse bone marrow derived hepatocyte formation in vivo. PLoS ONE. 2012;7:e33945 pubmed publisher
    ..These BMDHs have their own cell identity characterized by an expression pattern different from hematopoietic cells or hepatocytes. The role of these BMDHs in the liver requires further investigation. ..
  46. Chang C, Chang G, Chen H. A novel cyclic AMP/Epac1/CaMKI signaling cascade promotes GCM1 desumoylation and placental cell fusion. Mol Cell Biol. 2011;31:3820-31 pubmed publisher
    ..8-CPT-AM), an Epac activator, stimulates syncytin-1 and -2 gene expression and cell fusion of placental BeWo cells in a GCM1-dependent manner...
  47. Kemp K, Gray E, Wilkins A, Scolding N. Purkinje cell fusion and binucleate heterokaryon formation in multiple sclerosis cerebellum. Brain. 2012;135:2962-72 pubmed publisher
    ..Here, we have explored Purkinje cell fusion and heterokaryon formation in the human brain and the influence of central nervous system inflammation...
  48. Shinn Thomas J, Mohler W. New insights into the mechanisms and roles of cell-cell fusion. Int Rev Cell Mol Biol. 2011;289:149-209 pubmed publisher
    ..Some of these examples come from experimental systems where the discovery of molecules essential for cell fusion is sped by the approachability of the experimental organism itself...
  49. Aguilar P, Baylies M, Fleissner A, Helming L, Inoue N, Podbilewicz B, et al. Genetic basis of cell-cell fusion mechanisms. Trends Genet. 2013;29:427-37 pubmed publisher
    Cell-cell fusion in sexually reproducing organisms is a mechanism to merge gamete genomes and, in multicellular organisms, it is a strategy to sculpt organs, such as muscle, bone, and placenta...
  50. Tat P, Sumer H, Pralong D, Verma P. The efficiency of cell fusion-based reprogramming is affected by the somatic cell type and the in vitro age of somatic cells. Cell Reprogram. 2011;13:331-44 pubmed publisher
    b>Cell fusion is one approach that has been used to demonstrate nuclear reprogramming of somatic cells to a pluripotent-like state and is a useful tool for screening factors involved in reprogramming...
  51. Miyamoto H, Suzuki T, Miyauchi Y, Iwasaki R, Kobayashi T, Sato Y, et al. Osteoclast stimulatory transmembrane protein and dendritic cell–specific transmembrane protein cooperatively modulate cell–cell fusion to form osteoclasts and foreign body giant cells. J Bone Miner Res. 2012;27:1289-97 pubmed publisher
    Cell–cell fusion is a dynamic phenomenon promoting cytoskeletal reorganization and phenotypic changes...
  52. Ruebner M, Langbein M, Strissel P, Henke C, Schmidt D, Goecke T, et al. Regulation of the human endogenous retroviral Syncytin-1 and cell-cell fusion by the nuclear hormone receptors PPAR?/RXR? in placentogenesis. J Cell Biochem. 2012;113:2383-96 pubmed publisher
    Cytotrophoblast (CT) cell fusion into a syncytiotrophoblast is obligatory for placentation and mediated by the human endogenous retrovirus (HERV)-W envelope gene Syncytin-1...
  53. Diaz D, Recio J, Weruaga E, Alonso J. Mild cerebellar neurodegeneration of aged heterozygous PCD mice increases cell fusion of Purkinje and bone marrow-derived cells. Cell Transplant. 2012;21:1595-602 pubmed
    Bone marrow-derived cells have different plastic properties, especially regarding cell fusion, which increases with time and is prompted by tissue injury...