isolated heart preparation


Summary: Method of isolation and maintenance of a working heart for various cardiac measurements. The model allows for the separation of cardiac and systemic variables in the denervated heart.

Top Publications

  1. Vecsernyes M, Szokol M, Bombicz M, Priksz D, Gesztelyi R, Fulop G, et al. Alpha-Melanocyte-stimulating Hormone Induces Vasodilation and Exerts Cardioprotection Through the Heme-Oxygenase Pathway in Rat Hearts. J Cardiovasc Pharmacol. 2017;69:286-297 pubmed publisher
  2. Chou C, Chang P, Wei Y, Lee K. Optical Mapping Approaches on Muscleblind-Like Compound Knockout Mice for Understanding Mechanistic Insights Into Ventricular Arrhythmias in Myotonic Dystrophy. J Am Heart Assoc. 2017;6: pubmed publisher
    ..Optical mapping studies revealed prolonged action potential duration and slow conduction velocity in the DM mice, leading to vulnerability of spatially discordant alternans and ventricular arrhythmia induction to pacing. ..
  3. Rubi L, Kovar M, Zebedin Brandl E, Koenig X, Dominguez Rodriguez M, Todt H, et al. Modulation of the heart's electrical properties by the anticonvulsant drug retigabine. Toxicol Appl Pharmacol. 2017;329:309-317 pubmed publisher
    ..They are rather mediated by indirect actions at the level of the autonomic nervous system. ..
  4. Fulgenzi G, Tomassoni Ardori F, Babini L, Becker J, Barrick C, Puverel S, et al. BDNF modulates heart contraction force and long-term homeostasis through truncated TrkB.T1 receptor activation. J Cell Biol. 2015;210:1003-12 pubmed
  5. Li M, Hothi S, Salvage S, Jeevaratnam K, Grace A, Huang C. Arrhythmic effects of Epac-mediated ryanodine receptor activation in Langendorff-perfused murine hearts are associated with reduced conduction velocity. Clin Exp Pharmacol Physiol. 2017;44:686-692 pubmed publisher
    ..We thus demonstrate for the first time that acute RyR2 activation reversibly induces VT in specific association with reduced CV. ..
  6. Shattock M, Park K, Yang H, Lee A, Niederer S, MacLeod K, et al. Restitution slope is principally determined by steady-state action potential duration. Cardiovasc Res. 2017;113:817-828 pubmed publisher
    ..However, concomitant changes in tissue refractoriness act to reduce susceptibility to sustained VF. Dependence on steady-state APD may contribute to the failure of restitution slope to predict sudden cardiac death. ..
  7. Ragavan M, Kirpich A, Fu X, Burgess S, McIntyre L, Merritt M. A comprehensive analysis of myocardial substrate preference emphasizes the need for a synchronized fluxomic/metabolomic research design. Am J Physiol Heart Circ Physiol. 2017;312:H1215-H1223 pubmed publisher
    ..This observation leads to a key observation regarding mass balance in the TCA cycle; flux through a portion of the cycle can be drastically elevated without changing the O2 consumption. ..
  8. Tobisawa T, Yano T, Tanno M, Miki T, Kuno A, Kimura Y, et al. Insufficient activation of Akt upon reperfusion because of its novel modification by reduced PP2A-B55α contributes to enlargement of infarct size by chronic kidney disease. Basic Res Cardiol. 2017;112:31 pubmed publisher
  9. Li Y, Zhu G, Paolocci N, Zhang P, Takahashi C, Okumus N, et al. Heart Failure-Related Hyperphosphorylation in the Cardiac Troponin I C Terminus Has Divergent Effects on Cardiac Function In Vivo. Circ Heart Fail. 2017;10: pubmed publisher
    ..However, paradoxically, it preserves heart function after ischemia/reperfusion injury, potentially by decreasing proteolysis of cTnI. ..

More Information


  1. Cho J, Zhang R, Kilfoil P, Gallet R, de Couto G, Bresee C, et al. Delayed Repolarization Underlies Ventricular Arrhythmias in Rats With Heart Failure and Preserved Ejection Fraction. Circulation. 2017;136:2037-2050 pubmed publisher
    ..Our findings are consistent with the hypothesis that potassium current downregulation leads to abnormal repolarization in HFpEF, which in turn predisposes to VA and sudden cardiac death. ..
  2. Nederlof R, Denis S, Lauzier B, Rosiers C, Laakso M, Hagen J, et al. Acute detachment of hexokinase II from mitochondria modestly increases oxygen consumption of the intact mouse heart. Metabolism. 2017;72:66-74 pubmed publisher
    ..These results indicate that acute albeit not chronic changes in mitochondrial HKII modestly affect cardiac oxygen consumption and energy substrate metabolism. ..
  3. Renguet E, Ginion A, Gélinas R, Bultot L, Auquier J, Robillard Frayne I, et al. Metabolism and acetylation contribute to leucine-mediated inhibition of cardiac glucose uptake. Am J Physiol Heart Circ Physiol. 2017;313:H432-H445 pubmed publisher
    ..Listen to this article's corresponding podcast at ..
  4. Youssef N, Campbell S, Barr A, Gandhi M, Hunter B, Dolinsky V, et al. Hearts lacking plasma membrane KATP channels display changes in basal aerobic metabolic substrate preference and AMPK activity. Am J Physiol Heart Circ Physiol. 2017;313:H469-H478 pubmed publisher
    ..These results suggest that ATP-sensitive K+ channels may play a novel role in regulating metabolism in addition to their well-documented effects on ionic homeostasis during periods of stress. ..
  5. Boardman N, Hafstad A, Lund J, Rossvoll L, Aasum E. Exercise of obese mice induces cardioprotection and oxygen sparing in hearts exposed to high-fat load. Am J Physiol Heart Circ Physiol. 2017;313:H1054-H1062 pubmed publisher
    ..Myocardial oxygen sparing is associated with this effect, despite the general notion that high fat can decrease cardiac efficiency. This highlights the role of myocardial energetics during ischemic stress. ..
  6. Kawai S, Yamada T, Matsuura T, Funao T, Nishikawa K. Neuropathic pain attenuates ischemia reperfusion injury through ?2-adrenergic pathway. Life Sci. 2017;187:9-16 pubmed publisher
    ..The underlying mechanisms were possibly regulated by ?2-adrenergic activation and pro-survival kinase expression in cardiomyocytes. ..