paper chromatography


Summary: An analytical technique for resolution of a chemical mixture into its component compounds. Compounds are separated on an adsorbent paper (stationary phase) by their varied degree of solubility/mobility in the eluting solvent (mobile phase).

Top Publications

  1. Li H, Qiu T, Cao Y, Yang J, Huang Z. Pre-staining paper chromatography method for quantification of gamma-aminobutyric acid. J Chromatogr A. 2009;1216:5057-60 pubmed publisher
    The routine method of paper chromatography includes five steps: spotting, separating, drying, spraying/immersing and color development...
  2. Rahman M, Goegebuer T, De Leener K, Maes P, Matthijnssens J, Podder G, et al. Chromatography paper strip method for collection, transportation, and storage of rotavirus RNA in stool samples. J Clin Microbiol. 2004;42:1605-8 pubmed
    ..This convenient and inexpensive rotavirus sample collection system can be of use in epidemiological surveillance studies and vaccine trials. ..
  3. Van Herle A, Birnbaum J, Slomowitz L, Mayes D, Chandler D, Rosenblit P, et al. Paper chromatography prior to cortisol RIA allows for accurate use of the dexamethasone suppression test in chronic renal failure. Nephron. 1998;80:79-84 pubmed
    ..hormone, corticosterone, and cortisol by both direct radioimmunoassay (RIA) and RIA after paper chromatography. Utilizing the direct cortisol RIA, 4 of 6 patients with CRF exhibited blunted dexamethasone suppression, ..
  4. Wolbiś M, Nowak S, Kicel A. Polyphenolic compounds in Scopolia caucasica Kolesn. ex Kreyer (Solanaceae). Acta Pol Pharm. 2007;64:241-6 pubmed
    ..of coumarins, phenolic acids and flavonoids in the leaves and underground parts of Scopolia caucasica using paper chromatography and HPLC methods were described...
  5. Lalitha K, Rajendra Kulothungan S. Selective determination of mimosine and its dihydroxypyridinyl derivative in plant systems. Amino Acids. 2006;31:279-87 pubmed
    ..1 to 0.75 microM or DHP from 0.2 to 1.5 microM and with recoveries of 99.2 to 100.5%. ..
  6. Zlateva K, Maes P, Rahman M, Van Ranst M. Chromatography paper strip sampling of enteric adenoviruses type 40 and 41 positive stool specimens. Virol J. 2005;2:6 pubmed
  7. Tripathi M, Khanna S, Das M. A novel method for the determination of synthetic colors in ice cream samples. J AOAC Int. 2004;87:657-63 pubmed
    ..5% ammonia solution, the solution is evaporated to dryness, and the residue is dissolved in 60% ethanol for paper chromatography using trisodium citrate-ammonia-water (2 + 5 + 95, w/v/v) as the mobile phase...
  8. Yang H, Erdos E, Levin Y. A dipeptidyl carboxypeptidase that converts angiotensin I and inactivates bradykinin. Biochim Biophys Acta. 1970;214:374-6 pubmed
  9. Nelson L, Perrone J, DeRoos F, Stork C, Hoffman R. Aldicarb poisoning by an illicit rodenticide imported into the United States: Tres Pasitos. J Toxicol Clin Toxicol. 2001;39:447-52 pubmed
    ..This study characterizes the ongoing epidemic of acute cholinesterase inhibitor poisoning due to an illicit rodenticide and identifies its etiology...

More Information


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  3. Hao Z, Stowe E, Ahluwalia G, Baker D, Hebbler A, Chisena C, et al. Characterization of 2',3'-dideoxycytidine diphosphocholine and 2',3'-dideoxycytidine diphosphoethanolamine. Prominent phosphodiester metabolites of the anti-HIV nucleoside 2',3'-dideoxycytidine. Drug Metab Dispos. 1993;21:738-44 pubmed
    ..The possible role of these phosphodiesters in contributing to the unusual toxicity of ddCyd is discussed. ..
  4. Albers E, Gustafsson L, Niklasson C, Liden G. Distribution of 14C-labelled carbon from glucose and glutamate during anaerobic growth of Saccharomyces cerevisiae. Microbiology. 1998;144 ( Pt 6):1683-90 pubmed
    ..Separation of the amino acids from biomass by paper chromatography revealed that the glutamate family of amino acids (glutamic acid, glutamine, proline, arginine and lysine) ..
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    ..The metK strains are able to synthesize cyclopropane fatty acids, but the rate of their formation is slowed. Modification and restriction of phage 21 appears to be normal, suggesting that these strains are able to methylate DNA. ..
  8. Boese B, Breaker R. In vitro selection and characterization of cellulose-binding DNA aptamers. Nucleic Acids Res. 2007;35:6378-88 pubmed
    ..CELAPT 14 aptamer variants exhibit robust binding both to cellulose powder and paper. Also, an allosteric aptamer construct was engineered that exhibits ATP-mediated cellulose binding during paper chromatography.
  9. Iznaga Escobar N. Direct radiolabeling of monoclonal antibodies with rhenium-188 for radioimmunotherapy of solid tumors--a review of radiolabeling characteristics, quality control and in vitro stability studies. Appl Radiat Isot. 2001;54:399-406 pubmed
    ..Moreover, excellent methods have been developed to test the radionuclide, radiochemical purity and stability of 188Re-radioimmunoconjugates using high performance liquid chromatography (HPLC) and paper chromatography.
  10. Collinder E, Berge G, Grønvold B, Lindholm A, Midtved T, Norin E. Influence of bacitracin on microbial functions in the gastrointestinal tract of horses. Equine Vet J. 2000;32:345-50 pubmed
    ..Utilising gas chromatography, spectrophotometry, gel electrophoresis and paper chromatography, samples were analysed on biochemical markers reflecting the action of parts of the intestinal flora...
  11. Watanabe F, Katsura H, Takenaka S, Fujita T, Abe K, Tamura Y, et al. Pseudovitamin B(12) is the predominant cobamide of an algal health food, spirulina tablets. J Agric Food Chem. 1999;47:4736-41 pubmed
    ..leichmannii as a test organism and the binding of vitamin B(12) to the intrinsic factor. ..
  12. Banerjee S, Das T, Samuel G, Sarma H, Venkatesh M, Pillai M. A novel [186/188Re]-labelled porphyrin for targeted radiotherapy. Nucl Med Commun. 2001;22:1101-7 pubmed
    ..The complexation yield was >98% as estimated by paper chromatography in acetone and in saline...
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    ..The present study shows that this concept also applies to heparan sulfate biosynthesis in the intact cell. ..
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    ..It was impossible to accumulate UDP-MurNAc-pentapeptide in E. coli by the antibiotics penicillin and vancomycin. The hypothesis is put forward that the amount of this murein precursor is regulated by feedback inhibition. ..
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    ..5. A comparison between paper chromatography and high voltage paper electrophoresis in terms of effective analysis of amino acid patterns demonstrated ..
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    ..Four unique half-cystine residues occur in two peptides, one containing 11 and the other, at the C-terminus of the protein, 15 amino acids. These sequences are compared with published data on growth hormones from other species. ..
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    ..Methylglyoxal-resistant mutants derived from the glycerol-sensitive parental strain also became immune to glycerol. ..
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    ..These studies reveal that (166)Ho-EC could be a viable substitute for (188)Re compounds in radioactive liquid-filled balloon IVRT. ..
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  24. Mukerjea R, Robyt J. Starch biosynthesis: sucrose as a substrate for the synthesis of a highly branched component found in 12 varieties of starches. Carbohydr Res. 2003;338:1811-22 pubmed
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    ..and dolichol C55 (dolichol 11:dihydroundecaprenol) were separated by anion-exchange paper chromatography. Squalene, sterols, phospholipids, anionic glycolipids, and glycerol did not migrate as dolichyl phosphate, ..
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  27. Jha R, de Sousa S. Microplate assay for inhibitors of the transpeptidase activity of PBP1b of Escherichia coli. J Biomol Screen. 2006;11:1005-14 pubmed
    ..Moenomycin, a TG inhibitor, also inhibited TP activity. Because this is a true enzyme assay, it has the potential to detect novel, non-beta-lactam TP inhibitors and could lead to the discovery of new antibacterial agents. ..
  28. Shukla S, Atzei G, Sedda A, Rossi G, Cipriani C. Paper chromatographic and paper elettrophoretic study of the solution chemistry of Tc-99m-methylendiphosphonate and of Tc-99m-dimercaptosuccinate for improving the tumour-affinity of Tc-99m during scintigraphic imaging of cancer. Ann Chim. 2004;94:761-6 pubmed from the radionuclide uptake, the solution chemistry of these radiopharmaceuticals was studied by paper chromatography and paper electrophoresis in distilled water, in physiological saline, in NAHCO3, and ascorbic acid ..
  29. Rick P, Wolski S, Barr K, Ward S, Ramsay Sharer L. Accumulation of a lipid-linked intermediate involved in enterobacterial common antigen synthesis in Salmonella typhimurium mutants lacking dTDP-glucose pyrophosphorylase. J Bacteriol. 1988;170:4008-14 pubmed
    ..The data support the conclusion that accumulation of lipid II is responsible in some way for the hypersensitivity of delta rfbA mutants to SDS. ..
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    ..This study strongly suggests a nonluminal, cytoplasmic orientation of the N-acetylglucosaminyltransferases concerned with the biosynthesis of the first two intermediates of the dolichol pathway. ..
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    ..cerevisiae, which suggests a role for O-mannosylated proteins in the resistance mechanism. In addition, MNT1 is part of a multigene family whose members are presumed to be yeast Golgi mannosyltransferases. ..
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    ..Another alternative protocol uses sodium sulfite. ..
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    ..5%. ..
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    ..Variations in other pteridines were also observed. The mutant accumulates and later loses drosopterins in the abdominal fat body of adults. ..
  40. Blake R, Russell E. Hyperprolinemia and prolinuria in a new inbred strain of mice, PRO-Re. Science. 1972;176:809-11 pubmed
    ..In addition, mice of this PRO/Re strain exhibited a marked prolinuria, whereas the other 14 inbred strains had no proline detectable in their urine. ..
  41. Ramakrishnan M, Wengenack T, Kandimalla K, Curran G, Gilles E, Ramirez Alvarado M, et al. Selective contrast enhancement of individual Alzheimer's disease amyloid plaques using a polyamine and Gd-DOTA conjugated antibody fragment against fibrillar Abeta42 for magnetic resonance molecular imaging. Pharm Res. 2008;25:1861-72 pubmed publisher
  42. Gurgel L, Júnior O, Gil R, Gil L. Adsorption of Cu(II), Cd(II), and Pb(II) from aqueous single metal solutions by cellulose and mercerized cellulose chemically modified with succinic anhydride. Bioresour Technol. 2008;99:3077-83 pubmed
    ..Adsorption isotherms were developed using Langmuir model. Cell 6 in relation to cell 5 exhibited an increase in Qmax for Cu2+ (30.4 mg/g), Cd2+ (86.0 mg/g) and Pb2+ (205.9 mg/g)...
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    ..Challenges of industrial-scale manufacturing of therapeutics were considered in the development of this process. ..
  46. Elzainy T, Ali T. Nature and mode of action of NAD and ATP dephosphorylating enzyme from Aspergillus terreus DSM 826. Antonie Van Leeuwenhoek. 2003;84:255-60 pubmed
    ..Chromatographic detection of the intermediates and the products formed during the progression of NAD and ATP dephosphorylation by the most purified fraction of this enzyme was found to be consistent with the following mode of its action: ..
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  48. Saritha K, Nanda Kumar N. Qualitative detection of selenium in fortified soil and water samples by a paper chromatographic-carboxyl esterase enzyme inhibition technique. J Chromatogr A. 2001;919:223-8 pubmed
    ..Soil and water samples were fortified with sodium selenite and selenium dioxide. A procedure for preparing test solutions and conditions for paper chromatography was established.
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    ..LS23 7BQ, U.K., from whom copies may be obtained on the terms given in Biochem. J. (1973) 131, 5. ..
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