mercaptoethanol

Summary

Summary: A water-soluble thiol derived from hydrogen sulfide and ethanol. It is used as a reducing agent for disulfide bonds and to protect sulfhydryl groups from oxidation.

Top Publications

  1. De Matos D, Furnus C. The importance of having high glutathione (GSH) level after bovine in vitro maturation on embryo development effect of beta-mercaptoethanol, cysteine and cystine. Theriogenology. 2000;53:761-71 pubmed
    Supplementation of IVM medium with cysteamine, beta-mercaptoethanol, cysteine and cystine induced bovine oocyte glutathione (GSH) synthesis, but only the effect of cysteamine on the developmental competence of these oocytes was tested...
  2. Hung S, Cheng H, Pan C, Tsai M, Kao L, Ma H. In vitro differentiation of size-sieved stem cells into electrically active neural cells. Stem Cells. 2002;20:522-9 pubmed
    ..Five hours after exposure to antioxidant agents (beta-mercaptoethanol +/- retinoic acid) in serum-free conditions, SS cells expressed the protein for nestin, neuron-specific ..
  3. Takahashi M, Nagai T, Okamura N, Takahashi H, Okano A. Promoting effect of beta-mercaptoethanol on in vitro development under oxidative stress and cystine uptake of bovine embryos. Biol Reprod. 2002;66:562-7 pubmed
    The effects of beta-mercaptoethanol (beta-ME) on in vitro development under oxidative stress and cystine uptake of bovine embryos were investigated...
  4. McAlpin C, Cox C, Matyi S, Voorhees K. Enhanced matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of bacteriophage major capsid proteins with beta-mercaptoethanol pretreatment. Rapid Commun Mass Spectrom. 2010;24:11-4 pubmed publisher
    ..The findings presented here suggest that beta-mercaptoethanol reduction of the disulfide bonds linking phage MCPs prior to mass spectrometric analysis results in ..
  5. Nedambale T, Du F, Yang X, Tian X. Higher survival rate of vitrified and thawed in vitro produced bovine blastocysts following culture in defined medium supplemented with beta-mercaptoethanol. Anim Reprod Sci. 2006;93:61-75 pubmed
    ..defined culture media, up to blastocyst stage, and subsequently cultured in media supplemented with beta-mercaptoethanol (beta-ME) following blastocyst vitrification and thawing...
  6. Click R. Longevity of SLE-prone mice increased by dietary 2-mercaptoethanol via a mechanism imprinted within the first 28 days of life. Virulence. 2010;1:516-22 pubmed
    In the preceding report, moderately lived-mice fed dietary 2-mercaptoethanol (2-Me) had their life extended, whereas long-lived mice were found to have the quality of life improved, but not extended, and did not develop high fat-diet ..
  7. Whitaker B, Knight J. Exogenous gamma-glutamyl cycle compounds supplemented to in vitro maturation medium influence in vitro fertilization, culture, and viability parameters of porcine oocytes and embryos. Theriogenology. 2004;62:311-22 pubmed
    ..5, or 5 mM), l-glutamate (1, 2.5, or 5 mM), l-alpha-aminobutyrate (3.3mM), beta-mercaptoethanol (BME) (25 microM), l-cysteine and BME, or l-alpha-aminobutyrate and BME. Increases (P < 0...
  8. Click R. Dietary supplemented 2-mercaptoethanol prevents spontaneous and delays virally-induced murine mammary tumorigenesis. Cancer Biol Ther. 2013;14:521-6 pubmed publisher
    ..Mice from three strains with a high incidence for naturally occurring tumors were treated daily with 2-mercaptoethanol (2-Me) starting at weaning...
  9. Yamaguchi S, Funahashi H. Effect of the addition of beta-mercaptoethanol to a thawing solution supplemented with caffeine on the function of frozen-thawed boar sperm and on the fertility of sows after artificial insemination. Theriogenology. 2012;77:926-32 pubmed publisher
    ..The objective of the present study was to examine the effects of the addition of the antioxidant beta-mercaptoethanol (bME) and caffeine to the thawing solution on the function of frozen-thawed sperm, on the phagocytic activity ..

More Information

Publications61

  1. Songsasen N, Apimeteetumrong M. Effects of beta-mercaptoethanol on formation of pronuclei and developmental competence of swamp buffalo oocytes. Anim Reprod Sci. 2002;71:193-202 pubmed
    This study was conducted to determine the effect of supplementing maturation medium with beta-mercaptoethanol (betaME) on pronuclei formation and developmental competence of swamp buffalo oocytes...
  2. Orsatti L, Pallaoro M, Steinküler C, Orru S, Bonelli F. Reactivity of the NS2/3(907-1206)ASK(4) protein with beta-mercaptoethanol studied by electrospray ion trap mass spectrometry. Rapid Commun Mass Spectrom. 2002;16:1919-27 pubmed
    ..During routine protein manipulation, in the presence of 100 mM beta-mercaptoethanol and under denatured conditions, the protein was unexpectedly modified at its cysteine residues, and the ..
  3. Click R. Obesity, longevity, quality of life: alteration by dietary 2-mercaptoethanol. Virulence. 2010;1:509-15 pubmed
    ..murine immunological reactivity in tissue culture required a sulfhydryl compound; the most effective being 2-mercaptoethanol (2-Me)...
  4. Fujiwara N, Nakano M, Kato S, Yoshihara D, Ookawara T, Eguchi H, et al. Oxidative modification to cysteine sulfonic acid of Cys111 in human copper-zinc superoxide dismutase. J Biol Chem. 2007;282:35933-44 pubmed
    ..of the SOD1 protein, by comparing the oxidative susceptibility of recombinant human SOD1 modified with 2-mercaptoethanol at Cys(111) (2-ME-SOD1) to wild-type SOD1...
  5. Langhorst M, Genisyuerek S, Stuermer C. Accumulation of FlAsH/Lumio Green in active mitochondria can be reversed by beta-mercaptoethanol for specific staining of tetracysteine-tagged proteins. Histochem Cell Biol. 2006;125:743-7 pubmed
    ..Both ethanedithiol and beta-mercaptoethanol proved to be effective...
  6. Kobayashi M, Lee E, Fukui Y. Cysteamine or beta-mercaptoethanol added to a defined maturation medium improves blastocyst formation of porcine oocytes after intracytoplasmic sperm injection. Theriogenology. 2006;65:1191-9 pubmed
    ..study was carried out to investigate the effect of adding 100 microM cysteamine (Cys) or 100 microM beta-mercaptoethanol (beta-ME) to a defined maturation medium on in vitro maturation (IVM), and fertilization and developmental ..
  7. Bai J, Hou J, Guan H, Yan F, Cui X, Liu L, et al. Effect of 2-mercaptoethanol and cysteine supplementation during in vitro maturation on the developmental competence of oocytes from hormone-stimulated lambs. Theriogenology. 2008;70:758-64 pubmed publisher
    The objective was to determine the effect of 2-mercaptoethanol and cysteine on in vitro developmental competence of oocytes from lambs (4-8-week old) stimulated with eCG and pFSH...
  8. Hashem A, Hossein M, Woo J, Kim S, Kim J, Lee S, et al. Effect of potassium simplex optimization medium and NCSU23 supplemented with beta-mercaptoethanol and amino acids of in vitro fertilized porcine embryos. J Reprod Dev. 2006;52:591-9 pubmed
    ..optimization medium (KSOM) and North Carolina State University (NCSU)-23 medium supplemented with beta-mercaptoethanol (beta-ME) and amino acids (AA) on the developmental competence of porcine in vitro fertilized (IVF) embryos...
  9. Silva Ferreira A, Rodrigues P, Hogg T, Guedes De Pinho P. Influence of some technological parameters on the formation of dimethyl sulfide, 2-mercaptoethanol, methionol, and dimethyl sulfone in port wines. J Agric Food Chem. 2003;51:727-32 pubmed
    ..Four sulfur compounds were selected for this purpose: dimethyl sulfide, 2-mercaptoethanol, dimethyl sulfone, and methionol...
  10. Kadoma Y, Fujisawa S. Radical-scavenging activity of melatonin, either alone or in combination with vitamin E, ascorbate or 2-mercaptoethanol as co-antioxidants, using the induction period method. In Vivo. 2011;25:49-53 pubmed
    ..Melatonin shows antioxidant/prooxidant activity but its mechanism of action remains unknown...
  11. Bird S, Legge M, Walker R. Thiols stabilize cobblestone morphology of cultured mesothelial cells. Cell Biol Int. 2011;35:857-67 pubmed publisher
    ..with 2% serum, with 'plus' or without 'minus' L-Cys and compared with medium supplemented with either ?-ME (?-mercaptoethanol) (0.25 mmol/l) or the receptor tyrosine kinase ligand EGF (epidermal growth factor, 100 ng/ml)...
  12. Feugang J, de Roover R, Moens A, Léonard S, Dessy F, Donnay I. Addition of beta-mercaptoethanol or Trolox at the morula/blastocyst stage improves the quality of bovine blastocysts and prevents induction of apoptosis and degeneration by prooxidant agents. Theriogenology. 2004;61:71-90 pubmed
    This study was conducted to evaluate the effect of beta-mercaptoethanol (a stimulator of glutathione synthesis) and Trolox (an hydrosoluble analogue of Vitamin E) on bovine embryos cultured from the morula stage (Day 5 post-insemination; ..
  13. Abass E, Mansour D, el Mutasim M, Hussein M, el Harith A. Beta-mercaptoethanol-modified ELISA for diagnosis of visceral leishmaniasis. J Med Microbiol. 2006;55:1193-6 pubmed
    ..procedures similar to those of the direct agglutination test (DAT), an IgG ELISA employing intact beta-mercaptoethanol (beta-ME)-treated Leishmania donovani promastigotes was developed...
  14. Lu P, Blesch A, Tuszynski M. Induction of bone marrow stromal cells to neurons: differentiation, transdifferentiation, or artifact?. J Neurosci Res. 2004;77:174-91 pubmed
    ..to hours) induced to differentiate into neurons in vitro by relatively simple chemical means (using beta-mercaptoethanol [BME] or dimethylsulfoxide [DMSO] and butylated hydroxyanisol [BHA]; Woodbury et al. [ 2000] J. Neurosci. Res...
  15. Bagis H, Odaman Mercan H. Effect of chemically defined culture medium supplemented with beta-mercaptoethanol and amino acids on implantation and development of different stage in vivo- or in vitro-derived mouse embryos. Mol Reprod Dev. 2004;69:52-9 pubmed
    ..In the present study, the effects of beta-mercaptoethanol (Beta-ME) and amino acids (AA) on the development of mouse embryos obtained in vivo or in vitro at different ..
  16. Kim M, Fibrianto Y, Oh H, Jang G, Kim H, Lee K, et al. Effect of beta-mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected from dogs with different stages of the estrus cycle. J Vet Sci. 2004;5:253-8 pubmed
    Supplementation of beta-mercaptoethanol (beta-ME) in in vitro maturation (IVM) medium was shown to improve embryo development and quality in several species...
  17. Bagis H, Akkoc T, Taskin C, Arat S. Comparison of different cryopreservation techniques: higher survival and implantation rate of frozen-thawed mouse pronuclear embryos in the presence of beta-mercaptoethanol in post-thaw culture. Reprod Domest Anim. 2010;45:e332-7 pubmed publisher
    The objective of this study was to investigate the effects of beta-mercaptoethanol (?-ME) on post-thaw embryo developmental competence and implantation rate of mouse pronuclear (PN) embryos that were cryopreserved after slow freezing, ..
  18. Zhukov I, Ejchart A, Bierzynski A. Structural and motional changes induced in apo-S100A1 protein by the disulfide formation between its Cys 85 residue and beta-mercaptoethanol. Biochemistry. 2008;47:640-50 pubmed
    ..of S100A1 protein by disulfide bond formation with small thiols such as glutathione, cysteine, or beta-mercaptoethanol (betaME) leads to a dramatic increase of the protein affinity for calcium...
  19. Funahashi H. Effect of beta-mercaptoethanol during in vitro fertilization procedures on sperm penetration into porcine oocytes and the early development in vitro. Reproduction. 2005;130:889-98 pubmed
    This study was carried out to determine the effects of beta-mercaptoethanol (bME) during a transient co-culture of gametes for 10 min, and/or the following culture until 6-9 h after insemination, on sperm penetration of porcine in vitro ..
  20. Misra L, Lal P, Chaurasia N, Sangwan R, Sinha S, Tuli R. Selective reactivity of 2-mercaptoethanol with 5beta,6beta-epoxide in steroids from Withania somnifera. Steroids. 2008;73:245-51 pubmed
    2-Mercaptoethanol reacts selectively with the 5beta,6beta-epoxy steroids isolated from Withania somnifera substituting the epoxide by a six-membered oxyethylene-2'-thio ring whereas it failed to show such reactivity on 6alpha,7alpha-..
  21. Watanabe S, Hirano T, Grau E, Kaneko T. Osmosensitivity of prolactin cells is enhanced by the water channel aquaporin-3 in a euryhaline Mozambique tilapia (Oreochromis mossambicus). Am J Physiol Regul Integr Comp Physiol. 2009;296:R446-53 pubmed publisher
    ..The inhibitory effect of mercury was partially restored by beta-mercaptoethanol, whereas no effect of mercury was observed on PRL release stimulated by a depolarizing concentration of KCl, ..
  22. Du S, Liu H, Huang K. Influence of SelS gene silence on beta-Mercaptoethanol-mediated endoplasmic reticulum stress and cell apoptosis in HepG2 cells. Biochim Biophys Acta. 2010;1800:511-7 pubmed publisher
    ..In this report, the influence of selenite supplementation and SelS gene silence on beta-mercaptoethanol (beta-ME)-mediated ER stress and cell apoptosis in HepG2 cells were examined...
  23. Gupta M, Uhm S, Lee H. Effect of vitrification and beta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in vitro fertilization. Fertil Steril. 2010;93:2602-7 pubmed publisher
    To investigate the effect of vitrification and beta-mercaptoethanol (beta-ME) on reactive oxygen species (ROS) activity and in vitro development of oocytes vitrified before or after in vitro fertilization (IVF).
  24. Choe C, Shin Y, Kim E, Cho S, Kim H, Choi S, et al. Synergistic effects of glutathione and ?-mercaptoethanol treatment during in vitro maturation of porcine oocytes on early embryonic development in a culture system supplemented with L-cysteine. J Reprod Dev. 2010;56:575-82 pubmed
    ..Of the antioxidants tested (melatonin, glutathione (GSH), ?-mercaptoethanol (?-ME), N-acetylcysteine (NAC) and dithiothreitol (DTT)), addition of GSH (1 mM) or ?-ME (25 µM) ..
  25. Kumar A, Tiwari S, Thavaselvam D, Sathyaseelan K, Prakash A, Barua A, et al. Optimization and efficient purification of recombinant Omp28 protein of Brucella melitensis using Triton X-100 and ?-mercaptoethanol. Protein Expr Purif. 2012;83:226-32 pubmed publisher
    ..Different concentrations of Triton X-100 and ?-mercaptoethanol were optimized for the solubilization of inclusion bodies...
  26. Click R. Review: 2-mercaptoethanol alteration of in vitro immune functions of species other than murine. J Immunol Methods. 2014;402:1-8 pubmed publisher
    ..immune responses were reported to be dramatically enhanced by any of four xenobiotic, sulfhydryl compounds-2-mercaptoethanol (2-ME), dithiothreitol, glutathione, and l-cysteine; the most effective of the four was 2-ME...
  27. Piepponen T, Skujins A. Rapid and sensitive step gradient assays of glutamate, glycine, taurine and gamma-aminobutyric acid by high-performance liquid chromatography-fluorescence detection with o-phthalaldehyde-mercaptoethanol derivatization with an emphasis on microdialysi. J Chromatogr B Biomed Sci Appl. 2001;757:277-83 pubmed
    ..The amino acids were pre-column derivatized with o-phthalaldehyde-2-mercaptoethanol by using an automated refrigerated autoinjector...
  28. Zheng S, Newton G, Gonick G, Fahey R, Ward J. Radioprotection of DNA by thiols: relationship between the net charge on a thiol and its ability to protect DNA. Radiat Res. 1988;114:11-27 pubmed
    ..upon the net charge (Z) at pH 7 in the order WR 1065 (Z = +2) greater than cysteamine (Z = +1) greater than 2-mercaptoethanol (Z = 0) approximately equal to dithiothreitol (Z = 0) greater than GSH (Z = -1) approximately equal to 2-..
  29. Otagiri M, Kurisu G, Ui S, Takusagawa Y, Ohkuma M, Kudo T, et al. Crystal structure of meso-2,3-butanediol dehydrogenase in a complex with NAD+ and inhibitor mercaptoethanol at 1.7 A resolution for understanding of chiral substrate recognition mechanisms. J Biochem. 2001;129:205-8 pubmed
    ..structure of a ternary complex of meso-2,3-butanediol dehydrogenase with NAD+ and a competitive inhibitor, mercaptoethanol, has been determined at 1...
  30. Wang S, Zhao B, Zhang S, Ji H. Cloning, expression, purification, and characterization of AmphiTip30, a member of short-chain dehydrogenases/reductases family from the amphioxus Branchiostoma belcheri tsingtauense. Protein Expr Purif. 2008;57:63-71 pubmed
    ..Western blot and immunohistochemistry analysis confirmed that amphioxus has a native molecular mass of approximately 26 kDa, and TIP30 was strongly expressed in ovary. Finally, the initial function of TIP30 is discussed...
  31. Hashimoto F, Hayashi H. Presence and features of fatty acyl-CoA binding activity in rat hepatic peroxisomes. J Biochem. 2002;131:453-60 pubmed
    ..The activity also depended on the concentration of 2-mercaptoethanol, and a plateau of activity was unexpectedly found at 2-mercaptoethanol concentrations from 20 to 40 mM...
  32. Lee A, Chan A, Li T. Benzotrithiole 2-oxide: a new family of thiol-activated DNA-cleaving functionalities. Bioorg Med Chem Lett. 2002;12:3259-61 pubmed
    It was demonstrated in our studies that benzotrithiole 2-oxide was capable of causing efficient DNA cleavage in the presence of 2-mercaptoethanol or glutathione and exhibited potent cytotoxic properties against certain cancer cell lines.
  33. Wang J, Ruan H, Zhang H, Zhang Q, Zhang H, He G, et al. Characterization of a thermostable and acidic-tolerable beta-glucanase from aerobic fungi Trichoderma koningii ZJU-T. J Food Sci. 2007;72:C452-6 pubmed
    ..Denaturants, including sodium dodecyl sulfate (SDS) and mercaptoethanol, also inhibited beta-glucanase activity at a concentration of 5%...
  34. Garcia E, Llorente M, Hernando A, Kieffer R, Wieser H, Mendez E. Development of a general procedure for complete extraction of gliadins for heat processed and unheated foods. Eur J Gastroenterol Hepatol. 2005;17:529-39 pubmed
    ..This study was designed to develop a universal, extraction procedure capable of extracting the totality of gliadins from both unprocessed and heat processed foods for coeliac patients...
  35. Valerio A, Baldo G, Tessari P. A rapid method to determine plasma homocysteine concentration and enrichment by gas chromatography/mass spectrometry. Rapid Commun Mass Spectrom. 2005;19:561-7 pubmed
    ..First disulfide bonds were reduced by 2-mercaptoethanol, which allows the maintenance of the reduced status preventing the rebuilding of the disulfide bond...
  36. Kodali S, Galgoci A, Young K, Painter R, Silver L, Herath K, et al. Determination of selectivity and efficacy of fatty acid synthesis inhibitors. J Biol Chem. 2005;280:1669-77 pubmed
    ..showed an inhibition hierarchy of Zn2+ > Ca2+ > Mn2+ > Mg2+; no inhibition was found with beta-mercaptoethanol or dithiothreitol...
  37. Rawn D, Lau B, Niedzwiadek B, Lawrence J. Improved method for the determination of anatoxin-a and two of its metabolites in blue-green algae using liquid chromatography with fluorescence detection. J AOAC Int. 2005;88:1741-7 pubmed
    ..Addition of o-phthaldialdehyde (OPA) and mercaptoethanol to the extract before addition of the NBD-F resulted in the successful removal of primary amines from the ..
  38. Jung H, Seong H, Ha H. Direct interaction between NM23-H1 and macrophage migration inhibitory factor (MIF) is critical for alleviation of MIF-mediated suppression of p53 activity. J Biol Chem. 2008;283:32669-79 pubmed publisher
    ..NM23-H1-MIF complexes were dependent on reducing conditions, such as the presence of dithiothreitol or beta-mercaptoethanol, but not H(2)O(2)...
  39. Guo X, Zhao Y, Chang H, Wang C, E L, Zhang X, et al. Creation of engineered cardiac tissue in vitro from mouse embryonic stem cells. Circulation. 2006;113:2229-37 pubmed
  40. Kasana R, Yadav S. Isolation of a psychrotrophic Exiguobacterium sp. SKPB5 (MTCC 7803) and characterization of its alkaline protease. Curr Microbiol. 2007;54:224-9 pubmed
    ..a 20% increase in activity was observed when it was incubated in presence of reducing agents such as beta-mercaptoethanol and dithiothreitol. This suggests that the protease isolated from psychrotrophic Exiguobacterium sp...
  41. Paillasse M, Saffon N, Gornitzka H, Silvente Poirot S, Poirot M, de Medina P. Surprising unreactivity of cholesterol-5,6-epoxides towards nucleophiles. J Lipid Res. 2012;53:718-25 pubmed publisher
    ..These data rule out 5,6-EC acting as spontaneous alkylating agents. In addition, these data support the existence of a stereoselective metabolism of 5,6?-EC...
  42. Matsumoto M, Misawa S, Tsumoto K, Kumagai I, Hayashi H, Kobayashi Y. On-column refolding and characterization of soluble human interleukin-15 receptor alpha-chain produced in Escherichia coli. Protein Expr Purif. 2003;31:64-71 pubmed
    ..Thus, we succeeded in refolding shIL-15Ralpha to an active form on an affinity column...
  43. Harishchandran A, Pallavi B, Nagaraj R. A synthetic strategy for ON-resin amino acid specific multiple fatty acid acylation of peptides. Protein Pept Lett. 2002;9:411-7 pubmed
    ..The thiol protecting group was selectively deprotected and acylation was carried out on the resin-bound peptides. The strategy described in this report is applicable to any peptide sequence...
  44. Jiang Z, Hwang G, Xi Z, Goldberg I. Formation and structure of a novel enediyne-RNA base covalent adduct. J Am Chem Soc. 2002;124:3216-7 pubmed
    ..A novel mechanism involving adduction of the NCS-chrom C-6 radical, generated by 2-mercaptoethanol activation, to C-5 of the uracil at the U9 position of the RNA 11-mer, oxidation by dioxygen, reduction by ..
  45. Zhang D, Zhang F, Cui Y, Deng Q, Krause S, Zhou Y, et al. A label-free aptasensor for the sensitive and specific detection of cocaine using supramolecular aptamer fragments/target complex by electrochemical impedance spectroscopy. Talanta. 2012;92:65-71 pubmed publisher
    ..a gold electrode through modifying their 5' or 3' end with a thiolated group followed by the treatment with mercaptoethanol (MCE)...
  46. Suzuki M, Hirota M, Hagino S, Itagaki H, Aiba S. Evaluation of changes of cell-surface thiols as a new biomarker for in vitro sensitization test. Toxicol In Vitro. 2009;23:687-96 pubmed publisher
    ..Next, we confirmed that 2-mercaptoethanol in the culture medium and the differentiation state of THP-1 cells did not affect the changes of cell-surface ..
  47. Alfadhel M, AlShehhi W, Alshaalan H, Al Balwi M, Eyaid W, Eyaida W. Mucolipidosis II: first report from Saudi Arabia. Ann Saudi Med. 2013;33:382-6 pubmed publisher
    ..Clinicians should investigate any child presenting with neonatal hyperparathyroidism, craniosynostosis, skeletal deformities, and coarse facial features for MLII. ..
  48. Kochhar S, Kochhar V. Identification and characterization of a super-stable Cu-Zn SOD from leaves of turmeric (Curcuma longa L.). Planta. 2008;228:307-18 pubmed publisher
    ..The characterized novel isoform is a Cu-Zn SOD as is indicated by its sensitivity to both H2O2 and KCN. Indian, US and PCT patents have been filed and products are being developed using this hyperthermophilic enzyme...
  49. Kadoma Y, Fujisawa S. Radical-scavenging activity of dietary phytophenols in combination with co-antioxidants using the induction period method. Molecules. 2011;16:10457-70 pubmed publisher
    ..Furthermore, the EGC:ASDB (L-ascorbyl 2,6-dibutylate) or 2-ME (2-mercaptoethanol) combination showed the prooxidative effect...
  50. Fu R, Chen J, Li Y. [Influence of expression of transglutaminase on the growth of Lactococcus lactis]. Wei Sheng Wu Xue Bao. 2005;45:510-5 pubmed
  51. Kreinbring C, Remillard S, Hubbard P, Brodkin H, Leeper F, Hawksley D, et al. Structure of a eukaryotic thiaminase I. Proc Natl Acad Sci U S A. 2014;111:137-42 pubmed publisher
    ..active with amines such as aniline and veratrylamine as well as sulfhydryl compounds such as l-cysteine and ?-mercaptoethanol as cosubstrates. Despite significant differences in polypeptide sequence and length, we have shown that the N...
  52. Songsasen N, Yu I, Gomez M, Leibo S. Effects of meiosis-inhibiting agents and equine chorionic gonadotropin on nuclear maturation of canine oocytes. Mol Reprod Dev. 2003;65:435-45 pubmed
    ..The culture medium was TCM199 + 10 ng/ml epidermal growth factor supplemented with 25 microM beta-mercaptoethanol, 0.25 mM pyruvate, and 1.0 mM L-glutamine (Basal TCM)...