ammonium sulfate

Summary

Summary: Sulfuric acid diammonium salt. It is used in CHEMICAL FRACTIONATION of proteins.

Top Publications

  1. Yakhnin A, Vinokurov L, Surin A, Alakhov Y. Green fluorescent protein purification by organic extraction. Protein Expr Purif. 1998;14:382-6 pubmed
    ..An alternative method is described for the GFP purification without affinity extensions using organic extraction yielding a highly homogeneous protein indistinguishable in spectroscopic properties from that purified by previous methods...
  2. Karnchanatat A, Petsom A, Sangvanich P, Piaphukiew J, Whalley A, Reynolds C, et al. Purification and biochemical characterization of an extracellular beta-glucosidase from the wood-decaying fungus Daldinia eschscholzii (Ehrenb.:Fr.) Rehm. FEMS Microbiol Lett. 2007;270:162-70 pubmed
    ..Fr.) Rehm grown on 1.0% (w/v) carboxymethyl-cellulose using ammonium sulfate precipitation, ion-exchange, hydrophobic interaction and gel filtration chromatography...
  3. Koper T, Stark J, Habteselassie M, Norton J. Nitrification exhibits Haldane kinetics in an agricultural soil treated with ammonium sulfate or dairy-waste compost. FEMS Microbiol Ecol. 2010;74:316-22 pubmed publisher
    ..The K(m) values are comparable to those determined directly in other soils, but are substantially lower than those from most pure cultures of ammonia-oxidizing bacteria...
  4. Arakawa T, Tsumoto K, Ejima D, Kita Y, Yonezawa Y, Tokunaga M. Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography. J Biochem Biophys Methods. 2007;70:493-8 pubmed
    ..the protein flowed through the column; however, complete binding of BSA was achieved by the addition of 2 M ammonium sulfate (AS) to the column equilibration buffer and the sample...
  5. Ko K, Ahn D. Preparation of immunoglobulin Y from egg yolk using ammonium sulfate precipitation and ion exchange chromatography. Poult Sci. 2007;86:400-7 pubmed
    ..The concentrated solution was further purified using either cation exchange chromatography or ammonium sulfate precipitation...
  6. Arakawa T, Tsumoto K, Nagase K, Ejima D. The effects of arginine on protein binding and elution in hydrophobic interaction and ion-exchange chromatography. Protein Expr Purif. 2007;54:110-6 pubmed
    ..In the case of HIC, the proteins were bound to a phenyl-Sepharose column in the presence of ammonium sulfate (AS) with or without arginine and eluted with a descending concentration of AS...
  7. Chen J, Tetrault J, Ley A. Comparison of standard and new generation hydrophobic interaction chromatography resins in the monoclonal antibody purification process. J Chromatogr A. 2008;1177:272-81 pubmed
  8. Razani S, Mousavi S, Yeganeh H, Marc I. Fatty acid and carotenoid production by Sporobolomyces ruberrimus when using technical glycerol and ammonium sulfate. J Microbiol Biotechnol. 2007;17:1591-7 pubmed
    ..Sporobolomyces ruberrimus when using different concentrations of technical glycerol as the carbon source and ammonium sulfate as the nitrogen source...
  9. Zhao W, Zhang Y, Li Q. Indirect spectrophotometric determination of sodium ceftriaxone with n-propyl alcohol-ammonium sulfate-water system by extraction flotation of copper(II). Clin Chim Acta. 2008;391:80-4 pubmed publisher
    ..In order to circumvent this problem, a rapid and simple method for the indirect spectrophotometric determination of sodium ceftriaxone is reported...

More Information

Publications103 found, 100 shown here

  1. Hiraga K, Ueno Y, Oda K. Glutamate decarboxylase from Lactobacillus brevis: activation by ammonium sulfate. Biosci Biotechnol Biochem. 2008;72:1299-306 pubmed
    ..5-5.0, and 54 kDa on SDS-PAGE. The GAD activity and stability was significantly dependent on the ammonium sulfate concentration, as observed in authentic GAD...
  2. Park J, Lee S, Song J, Joo J, Chung M, Kim S, et al. Proteomic analysis of Helicobacter pylori cellular proteins fractionated by ammonium sulfate precipitation. Electrophoresis. 2008;29:2891-903 pubmed publisher
    ..pylori strain 26695 was subjected to protein fractionation with stepwise concentrations of ammonium sulfate and the proteins were displayed by 2-DE...
  3. Chen J, Lin A, Chen Z, Wang W, Zhang T, Cai H, et al. Ammonium sulfate gradient loading of brucine into liposomes: effect of phospholipid composition on entrapment efficiency and physicochemical properties in vitro. Drug Dev Ind Pharm. 2010;36:245-53 pubmed publisher
    ..We developed a novel liposome-based brucine formulation that was composed of soybean phosphatidylcholine (SPC) and hydrogenated soybean phosphatidylcholine (HSPC)...
  4. Drisdell W, Saykally R, Cohen R. On the evaporation of ammonium sulfate solution. Proc Natl Acad Sci U S A. 2009;106:18897-901 pubmed publisher
    ..We measured the cooling rate of 3 M ammonium sulfate droplets undergoing free evaporation via Raman thermometry. Analysis of the measurements yields a value of 0...
  5. Deng B, Liu Y, Yin H, Ning X, Lu H, Ye L, et al. Determination of ultra-trace formaldehyde in air using ammonium sulfate as derivatization reagent and capillary electrophoresis coupled with on-line electrochemiluminescence detection. Talanta. 2012;91:128-33 pubmed publisher
    ..The relative standard deviations of peak height and migration time for six consecutive injection of 1 ng/mL formaldehyde derivative were 0.9% and 0.8%, respectively. The recoveries of formaldehyde in air were between 99.3% and 101%...
  6. Oh S, Kim W, Hwang T, Han H, Lim S, Kim W. Development of an ammonium sulfate DNA extraction method for obtaining amplifiable DNA in a small number of cells and its application to clinical specimens. Biomed Res Int. 2013;2013:546727 pubmed publisher
    ..We developed an ammonium sulfate DNA extraction method (A) and compared it with two other methods (B and C)...
  7. Sharma S, Gupta M. Purification of phospholipase D from Dacus carota by three-phase partitioning and its characterization. Protein Expr Purif. 2001;21:310-6 pubmed
    ..5 and was unstable above 30 degrees C. Kinetic studies showed a K(m) value of 9.5 mM and a V(max) of 0.35 mL min(-1). The enzyme purified by three-phase partitioning was found to resolve into two isoenzymes on a DEAE-cellulose column...
  8. Selvakumar N, Rahman F, Garg R, Rajasekaran S, Mohan N, Thyagarajan K, et al. Evaluation of the phenol ammonium sulfate sedimentation smear microscopy method for diagnosis of pulmonary tuberculosis. J Clin Microbiol. 2002;40:3017-20 pubmed
    We compared the sensitivity and specificity of the phenol ammonium sulfate (PhAS) sediment smear microscopy method for detection of acid-fast bacilli with those of direct smear microscopy, using culture results for Mycobacterium ..
  9. Liu J, Hong R, Cheng W, Hong K, Chang F, Tseng Y. Simple and efficient liposomal encapsulation of topotecan by ammonium sulfate gradient: stability, pharmacokinetic and therapeutic evaluation. Anticancer Drugs. 2002;13:709-17 pubmed
    ..With an aim to improve on this drawback, we herein describe a method that utilizes the ammonium sulfate gradient to entrap TPT into liposomes...
  10. Maeda R, da Silva M, Santa Anna L, Pereira N. Nitrogen source optimization for cellulase production by Penicillium funiculosum, using a sequential experimental design methodology and the desirability function. Appl Biochem Biotechnol. 2010;161:411-22 pubmed publisher
    ..The following nitrogen sources were evaluated: urea, ammonium sulfate, peptone, and yeast extract...
  11. Li C, Wang C, Yang H, Zhao X, Wei N, Cui J. Liposomal topotecan formulation with a low polyethylene glycol grafting density: pharmacokinetics and antitumour activity. J Pharm Pharmacol. 2012;64:372-82 pubmed publisher
    ..In this study, a liposomal topotecan formulation with a low PEG grafting density was prepared and its pharmacokinetics, acute toxicity and antitumour effect were investigated...
  12. Nadar S, Rathod V. Magnetic macromolecular cross linked enzyme aggregates (CLEAs) of glucoamylase. Enzyme Microb Technol. 2016;83:78-87 pubmed publisher
    ..Glucoamylase magnetic macromolecular CLEAs prepared by precipitation in presence of AFMNs by ammonium sulfate were subsequently cross linked by dialdehydic pectin. After cross-linked by pectin, 95...
  13. Alves C, Simcikova M, Brito L, Monteiro G, Prazeres D. Development of a nicking endonuclease-assisted method for the purification of minicircles. J Chromatogr A. 2016;1443:136-44 pubmed publisher
    ..recombination of PPs into target MC, cell harvesting and alkaline lysis, precipitation with isopropanol and ammonium sulfate and diafiltration/concentration by microfiltration. Next, an in vitro digestion step was carried out with Nb...
  14. Ding H, Zeng Q, Zhou L, Yu Y, Chen B. Biochemical and Structural Insights into a Novel Thermostable ?-1,3-Galactosidase from Marinomonas sp. BSi20414. Mar Drugs. 2017;15: pubmed publisher
    ..BSi20414 isolated from Arctic sea ice by ammonium sulfate precipitation and anion exchange chromatography, resulting in an 8...
  15. Zaraî Jaouadi N, Rekik H, Ben Elhoul M, Zohra Rahem F, Hila C, Ben Aicha H, et al. A novel keratinase from Bacillus tequilensis strain Q7 with promising potential for the leather bating process. Int J Biol Macromol. 2015;79:952-64 pubmed publisher
    ..Pure protein was obtained after ammonium sulfate fractionation (30-60%), followed by Mono S Sepharose cation-exchange chromatography...
  16. Zordan C, Pennington M, Johnston M. Elemental composition of nanoparticles with the nano aerosol mass spectrometer. Anal Chem. 2010;82:8034-8 pubmed publisher
    ..A method to deconvolute these ion signals using sucrose and ammonium sulfate as calibrants is presented...
  17. Poltronieri A, Patrini L, Pigatto P, Riboldi L, Marsili C, Previdi M, et al. [Occupational allergic "march". Rapid evolution of contact dermatitis to ammonium persulfate into airborne contact dermatitis with rhinitis and asthma in a hairdresser]. Med Lav. 2010;101:403-8 pubmed
    ..The described case summarizes the evolution from contact dermatitis to inhalation allergy, suggesting the occurrence of an allergic "march" for occupational allergy. ..
  18. Zhu L, Zhong Z, Yang H, Wang C. Effect of MoO3 on vanadium based catalysts for the selective catalytic reduction of NOx with NH3 at low temperature. J Environ Sci (China). 2017;56:169-179 pubmed publisher
    ..Water vapor inhibited the NOx conversion at low temperature. Deposition of ammonium sulfate or bisulfate might be main reason for the loss of catalytic activity in the presence of SO2 at low ..
  19. Odeniyi O, Adeola O. Production and characterization of polyhydroxyalkanoic acid from Bacillus thuringiensis using different carbon substrates. Int J Biol Macromol. 2017;104:407-413 pubmed publisher
    ..Glucose and ammonium sulfate supplementation afforded the optimum cultural conditions for cell growth and PHA accumulation yielding 0...
  20. Hassan Y, He J, Perilla N, Tang K, Karlovsky P, Zhou T. The enzymatic epimerization of deoxynivalenol by Devosia mutans proceeds through the formation of 3-keto-DON intermediate. Sci Rep. 2017;7:6929 pubmed publisher
    ..Chemically synthesized 3-keto-DON incubated with D. mutans protein fractions enriched by ammonium sulfate precipitation at 35-55% saturation selectively reduced 3-keto-DON to 3-epi-DON, but fell short of supporting ..
  21. Nájera J, Horn A. Infrared spectroscopic study of the effect of oleic acid on the deliquescence behaviour of ammonium sulfate aerosol particles. Phys Chem Chem Phys. 2009;11:483-94 pubmed publisher
    ..assess the impact of fatty acids on the hygroscopic properties of atmospheric aerosol particles, (NH4)2SO4 (ammonium sulfate) and oleic acid (cis-9-octadecenoic acid) were chosen to perform this study as components of the particle ..
  22. Maischberger T, Leitner E, Nitisinprasert S, Juajun O, Yamabhai M, Nguyen T, et al. Beta-galactosidase from Lactobacillus pentosus: purification, characterization and formation of galacto-oligosaccharides. Biotechnol J. 2010;5:838-47 pubmed publisher
  23. Kasabe P, Mali G, Dandge P. Assessment of alkaline cholesterol oxidase purified from Rhodococcus sp. PKPD-CL for its halo tolerance, detergent and organic solvent stability. Protein Expr Purif. 2015;116:30-41 pubmed publisher
    ..It has apparent molecular weight of 60 kDa and was purified 59 fold by using 60% saturated ammonium sulfate fractionation, anion exchange followed by size exclusion chromatographic techniques with 37% recovery...
  24. Wong J, Nenes A, Weber R. Changes in Light Absorptivity of Molecular Weight Separated Brown Carbon Due to Photolytic Aging. Environ Sci Technol. 2017;51:8414-8421 pubmed publisher
    ..we study how the light absorptivity of BrC from wood smoke and secondary BrC generated from the reaction of ammonium sulfate with methylglyoxal changes under photolytic aging by UVA radiation in the aqueous phase...
  25. Abeyrathne E, Lee H, Ham J, Ahn D. Separation of ovotransferrin from chicken egg white without using organic solvents. Poult Sci. 2013;92:1091-7 pubmed publisher
    ..5 to 5.0. The resulting supernatant was added to different ratios of ammonium sulfate and citric acid, and then centrifuged after holding overnight at 4°C...
  26. Huang H, Wu J, Lin X, Li L, Shang S, Yuen M, et al. Self-assembly of polypyrrole/chitosan composite hydrogels. Carbohydr Polym. 2013;95:72-6 pubmed publisher
    ..This method may open a new opportunity for the fabrication of composite hydrogels associating the biomacromolecules and conducting polymers, and the improvement of the comprehensive performance of the resulting products. ..
  27. Li K, Zhu C, Zhang L, Zhu X. Study on pyrolysis characteristics of lignocellulosic biomass impregnated with ammonia source. Bioresour Technol. 2016;209:142-7 pubmed publisher
    ..characteristics of rice husk impregnated with different kinds of ammonia source (ammonium acetate, urea, ammonium sulfate and ammonium dihydrogen phosphate) in a fixed bed reactor...
  28. Bayrak A, Bayrak T, Demirpence E, Kilinc K. Differential hydrolysis of homocysteine thiolactone by purified human serum (192)Q and (192)R PON1 isoenzymes. J Chromatogr B Analyt Technol Biomed Life Sci. 2011;879:49-55 pubmed publisher
    ..PON1 isoenzymes were purified from the respective phenotype human serum, using a protocol consisting of ammonium sulfate precipitation and four chromatography steps: gel filtration, ion-exchange, non-specific affinity, and a ..
  29. Horvatić A, Dodig I, Vuletić T, Pavoković D, Hamersak Z, Butorac A, et al. Comparison between enhanced MALDI in-source decay by ammonium persulfate and N- or C-terminal derivatization methods for detailed peptide structure determination. Anal Chem. 2013;85:3940-7 pubmed publisher
  30. Saranya V, Rajeswari V, Abirami P, Poornimakkani K, Suguna P, Shenbagarathai R. Statistical media design for efficient polyhydroxyalkanoate production in Pseudomonas sp. MNNG-S. Prep Biochem Biotechnol. 2016;46:446-53 pubmed publisher
    ..based on the interactive effect of five significant variables (sucrose; potassium dihydrogen phosphate; ammonium sulfate; magnesium sulfate; trace elements)...
  31. Parasar P, Wilhelm A, Rutigliano H, Thomas A, Teng L, Shi B, et al. Expression of bovine non-classical major histocompatibility complex class I proteins in mouse P815 and human K562 cells. Res Vet Sci. 2016;107:161-170 pubmed publisher
    ..Furthermore, it demonstrated that BoLA-NC1*00401 is a secreted isoform and that significant quantities of membrane associated MHC-I proteins can be shed from the cell membrane. ..
  32. Li H, Wang D, Xu A, Li S, Jin S, Wu D. High level expression and purification of active recombinant human interleukin-8 in Pichia pastoris. Protein Expr Purif. 2009;68:60-4 pubmed publisher
    ..The process includes precipitation with 80% saturation ammonium sulfate and CM Sepharose ion exchange chromatography, yielding 30 mg/L purified rhIL-8 at over 95% purity...
  33. Morais Zani K, Tanaka A, Tanaka Azevedo A. Isolation of Bothrops jararaca snake antithrombin from the supernatant of fibrinogen purification. J Biomol Tech. 2009;20:249-52 pubmed
    ..jararaca plasma using a discarded material. This feature is important, considering the limitation of material, such as snake plasma, and could also be useful to obtain pure plasma proteins from other animals, including human plasma...
  34. El Kimary E, Hamdy D, Mourad S, Barary M. HPTLC Determination of Three Gliptins in Binary Mixtures with Metformin. J Chromatogr Sci. 2016;54:79-87 pubmed publisher
    ..5% w/v aqueous ammonium sulfate (8 : 2, v/v) as mobile phase...
  35. Mukhopadhyay M, Banerjee R. Purification and biochemical characterization of a newly produced yellow laccase from Lentinus squarrosulus MR13. 3 Biotech. 2015;5:227-236 pubmed publisher
    ..The yellow laccase was purified by a factor of 12.67-fold by ammonium sulfate precipitation, anion exchange chromatography and gel filtration chromatography to a specific activity of 3,..
  36. Qin B, Liu X, Cui H, Ma Y, Wang Z, Han J. Aqueous two-phase assisted by ultrasound for the extraction of anthocyanins from Lycium ruthenicum Murr. Prep Biochem Biotechnol. 2017;47:881-888 pubmed publisher
    ..An ethanol/ammonium sulfate system was chosen for the aqueous two-phase system due to its fine partitioning and recycling behaviors...
  37. O Connell R, White I, Mc Fadden J, White J. Hairdressers with dermatitis should always be patch tested regardless of atopy status. Contact Dermatitis. 2010;62:177-81 pubmed publisher
    ..It is necessary to patch test hairdressers with dermatitis, regardless of a history of atopy. Strategies to reduce prevalence of allergic contact dermatitis are required. ..
  38. Kim C, Cho Y, Byun J, Yun J. Mathematical approach to determine the linear attenuation coefficient without collimator in gamma-ray spectrometry. Radiat Prot Dosimetry. 2013;155:236-40 pubmed publisher
    ..The linear attenuation coefficient can be calculated within ?4 % of difference in the cylindrical sample at energies of 59.5, 121.8, 244.7, 344.2 and 444.0 keV. ..
  39. Abu Naim A, Umar A, Sanagi M, Basaruddin N. Chemical modification of chitin by grafting with polystyrene using ammonium persulfate initiator. Carbohydr Polym. 2013;98:1618-23 pubmed publisher
    ..Gel permeation chromatography (GPC) analysis carried out on the hydrolyzed graft copolymer showed that the Mn and Mw were 6.3395×10(4) g/mol and 1.69283×10(5) g/mol, respectively, with polydispersity index of 2.7. ..
  40. Potprommanee L, Wang X, Han Y, Nyobe D, Peng Y, Huang Q, et al. Characterization of a thermophilic cellulase from Geobacillus sp. HTA426, an efficient cellulase-producer on alkali pretreated of lignocellulosic biomass. PLoS ONE. 2017;12:e0175004 pubmed publisher
    ..HTA426. The cellulase enzyme produced by the Geobacillus sp. HTA426 was purified through ammonium sulfate precipitation and ion exchange chromatography, with the recovery yield and fold purification of 10.14% and 5...
  41. Liu Y, Lu F, Li Y, Yin X, Wang Y, Gao C. Characterisation of mutagenised acid-resistant alpha-amylase expressed in Bacillus subtilis WB600. Appl Microbiol Biotechnol. 2008;78:85-94 pubmed
    ..The optimum pH and stable range of pH with the mutagenised protein was 4.5 and 4.0 to 6.5, respectively, compared with pH 6.5 and 5.5 to 7.0 as the favorite pH and pH stability range of the native protein. ..
  42. Woerner M, Zamponi F, Ansari Z, Dreyer J, Freyer B, Prémont Schwarz M, et al. Concerted electron and proton transfer in ionic crystals mapped by femtosecond x-ray powder diffraction. J Chem Phys. 2010;133:064509 pubmed publisher
    ..so far unknown concerted transfer of electrons and protons in a prototype material, the hydrogen-bonded ionic ammonium sulfate [(NH(4))(2)SO(4)]...
  43. DiScipio R, Liddington R, Schraufstatter I. Fibulin-1 purification from human plasma using affinity chromatography on Factor H-Sepharose. Protein Expr Purif. 2016;121:118-24 pubmed publisher
    ..involve removal of the cryoglobulin and the vitamin K dependent proteins followed by polyethylene glycol and ammonium sulfate precipitations, DEAE-Sephadex column chromatography and finally Factor H-Sepharose affinity purification...
  44. Mulay Y, Deopurkar R. Purification, Characterization of Amylase from Indigenously Isolated Aureobasidium pullulans Cau 19 and Its Bioconjugates with Gold Nanoparticles. Appl Biochem Biotechnol. 2018;184:644-658 pubmed publisher
    The amylase from Aureobasidium pullulans Cau 19 was purified by ammonium sulfate precipitation and Sephadex G-100 chromatography with a 9.25-fold increase in specific activity as compared to crude enzyme...
  45. Schwarz J, Makeš O, Ondracek J, Cusack M, Talbot N, Vodicka P, et al. Single Usage of a Kitchen Degreaser Can Alter Indoor Aerosol Composition for Days. Environ Sci Technol. 2017;51:5907-5912 pubmed publisher
    ..MEA remaining on the cleaned surfaces and on a wiping paper towel in a trash can was able to transform ammonium sulfate and ammonium nitrate into (MEA)2SO4 and (MEA)NO3...
  46. Kurosawa K, Boccazzi P, de Almeida N, Sinskey A. High-cell-density batch fermentation of Rhodococcus opacus PD630 using a high glucose concentration for triacylglycerol production. J Biotechnol. 2010;147:212-8 pubmed publisher
    ..8) yields 77.6 g l(-1) of cell dry weight composed of approximately 38% TAGs indicating that this strain holds great potential as a future source of industrial biodiesel on starchy cellulosic feedstocks that are glucose polymers. ..
  47. Darcy E, Leonard P, Fitzgerald J, Danaher M, O Kennedy R. Purification of antibodies using affinity chromatography. Methods Mol Biol. 2011;681:369-82 pubmed publisher
    ..Examples are provided for the use of proteins A and G. In addition, tables are provided that allow the reader to select the most appropriate protein for use in the isolation of their antibody. ..
  48. Johnson H, Lenhoff A. Characterization and suitability of therapeutic antibody dense phases for subcutaneous delivery. Mol Pharm. 2013;10:3582-91 pubmed publisher
    ..Both crystalline and gel particles had protein concentrations of 100-180 mg/mL within the dense phase. Protein stored within the dense phase was recoverable after 40 days of incubation at room temperature or 4 °C. ..
  49. Wu X, Kong F, Huang M, Yu S. Effects of pH on the formation of 4(5)-Methylimidazole in glucose/ammonium sulfate and glucose/ammonium sulfite caramel model reactions. Food Res Int. 2015;76:661-665 pubmed publisher
    ..Glucose/ammonium sulfate and glucose/ammonium sulfite reaction systems with initial pH conditions 4.9, 5.9, 6.9, 8.0 and 8...
  50. Jiang X, Qi Y, Wang S, Tian X. New amphoteric flocculant containing beta-cyclodextrin, synthesis, characterization and decolorization properties. J Hazard Mater. 2010;173:298-304 pubmed publisher
  51. Ariz I, Artola E, Asensio A, Cruchaga S, Aparicio Tejo P, Moran J. High irradiance increases NH(4)(+) tolerance in Pisum sativum: Higher carbon and energy availability improve ion balance but not N assimilation. J Plant Physiol. 2011;168:1009-15 pubmed publisher
  52. Baker L, Stofan J, Lukaski H, Horswill C. Exercise-induced trace mineral element concentration in regional versus whole-body wash-down sweat. Int J Sport Nutr Exerc Metab. 2011;21:233-9 pubmed
    ..After exercise, the subjects and cycle ergometer were washed with 5 L of 15-mM ammonium sulfate solution to collect all sweat minerals and determine the volume of unevaporated sweat...
  53. Crews C, Hasnip S, Roberts D, Castle L. Factors affecting the analysis of furan in heated foods. Food Addit Contam. 2007;24 Suppl 1:108-13 pubmed
    ..Quantification based on standard additions or on external calibration gave comparable results for foods containing furan at relatively low, moderate and high levels...
  54. Wu S, Wu L, Shi Q, Wang Z, Chen X, Li Y. Effects of a new nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on nitrate and potassium leaching in two soils. J Environ Sci (China). 2007;19:841-7 pubmed
    ..Above results indicate that DMPP is a good nitrification inhibitor, the efficiency of DMPP seems better in the sandy loam soil than in the clay loam soil and lasts longer...
  55. Satar R, Husain Q. Adsorption of peroxidase on Celite 545 directly from ammonium sulfate fractionated white radish (Raphanus sativus) proteins. Biotechnol J. 2009;4:408-16 pubmed publisher
    This paper demonstrates the direct immobilization of peroxidase from ammonium sulfate fractionated white radish proteins on an inorganic support, Celite 545. The adsorbed peroxidase was crosslinked by using glutaraldehyde...
  56. Moreno Jiménez E, Peñalosa J, Esteban E, Bernal M. Feasibility of arsenic phytostabilisation using Mediterranean shrubs: impact of root mineralisation on As availability in soils. J Environ Monit. 2009;11:1375-80 pubmed publisher
    ..In this respect, the risk of As mobilisation due to root decomposition was found to be low, confirming the prospects for long-term phytomanagement and phytostabilisation of arsenic...
  57. Liu X, Ren B, Chen M, Wang H, Kokare C, Zhou X, et al. Production and characterization of a group of bioemulsifiers from the marine Bacillus velezensis strain H3. Appl Microbiol Biotechnol. 2010;87:1881-93 pubmed publisher
    ..0 and 2% NaCl by using starch as the carbon source and ammonium sulfate as the nitrogen source...
  58. Clegg S, Wexler A. Densities and apparent molar volumes of atmospherically important electrolyte solutions. 1. The solutes H2SO4, HNO3, HCl, Na2SO4, NaNO3, NaCl, (NH4)2SO4, NH4NO3, and NH4Cl from 0 to 50 °C, including extrapolations to very low temperature and to the p. J Phys Chem A. 2011;115:3393-460 pubmed publisher
    ..aim.env.uea.ac.uk/aim/aim.php) together with densities of the solid salts and hydrates...
  59. Stojićević I, Dimitrijevic L, Dovezenski N, Zivkovic I, Petrusic V, Marinković E, et al. Tetanus toxoid purification: chromatographic procedures as an alternative to ammonium-sulphate precipitation. J Chromatogr B Analyt Technol Biomed Life Sci. 2011;879:2213-9 pubmed publisher
  60. Khosravi K, Hoque M, Dimock B, Hintelmann H, Metcalfe C. A novel approach for determining total titanium from titanium dioxide nanoparticles suspended in water and biosolids by digestion with ammonium persulfate. Anal Chim Acta. 2012;713:86-91 pubmed publisher
    ..The reactions between persulfate ions and TiO(2) were evaluated using stoichiometric methods and FTIR and XRD analysis. A formula for the fusing reaction is proposed that involves the formation of sulfate radicals...
  61. Miladi H, Soukri A, Bakhrouf A, Ammar E. Expression of ferritin-like protein in Listeria monocytogenes after cold and freezing stress. Folia Microbiol (Praha). 2012;57:551-6 pubmed publisher
    ..Flp have a molecular mass of about 18 kDa, as observed on SDS-PAGE. The purification procedure including ammonium sulfate fractionation was used...
  62. Kramer R, Shende V, Motl N, Pace C, Scholtz J. Toward a molecular understanding of protein solubility: increased negative surface charge correlates with increased solubility. Biophys J. 2012;102:1907-15 pubmed publisher
    ..Here, we compare the use of representatives from two classes of precipitants, ammonium sulfate and polyethylene glycol 8000, by measuring the solubility of seven proteins...
  63. Bünsow J, Johannsmann D. Electrochemically produced responsive hydrogel films: influence of added salt on thickness and morphology. J Colloid Interface Sci. 2008;326:61-5 pubmed publisher
    ..When the films were produced in the presence of a destabilizing salt (such as ammonium sulfate) one also observes larger features, resembling wrinkles...
  64. Ekinci D, Beydemir S. Evaluation of the impacts of antibiotic drugs on PON 1; a major bioscavenger against cardiovascular diseases. Eur J Pharmacol. 2009;617:84-9 pubmed publisher
    ..The antibiotics exhibited different inhibition mechanisms. We concluded that usage of these antibiotics would be very dangerous in some cases...
  65. Survase S, Annapure U, Singhal R. Statistical optimization for improved production of cyclosporin a in solid-state fermentation. J Microbiol Biotechnol. 2009;19:1385-92 pubmed
    ..Based on the Plackett-Burman design, glycerol, ammonium sulfate, FeCl3, and inoculum size were selected for further optimization by response surface methodology (RSM)...
  66. Cheng K, Demirci A, Catchmark J. Enhanced pullulan production in a biofilm reactor by using response surface methodology. J Ind Microbiol Biotechnol. 2010;37:587-94 pubmed publisher
    ..5 centipoise (cP), which is higher than the commercial pullulan and related to its molecular weight. Fourier transform infrared spectroscopy (FTIR) also suggested that the produced exopolysaccharide was pullulan...
  67. Chen G, Wu X, Zhou W, Li L. Preparation and assay of C-glucosyltransferase from roots of Pueraria lobata. J Environ Biol. 2010;31:655-60 pubmed
    ..lobata, while no enzyme activity was detected in leaves of N. tabacum. It was the first time that activity of C-glucosyltransferase, which transforms isoliquiritigenin to puerarin, was detected in P. lobata...
  68. Razon L. Life cycle energy and greenhouse gas profile of a process for the production of ammonium sulfate from nitrogen-fixing photosynthetic cyanobacteria. Bioresour Technol. 2012;107:339-46 pubmed publisher
    ..and exopolysaccharides and convert these to biogas; strip and convert the ammonia from the biogas residue to ammonium sulfate; dry the ammonium sulfate solution to ammonium sulfate crystals and transport the finished product...
  69. Mall R, Naik G, MINA U, Mishra S. Purification and characterization of a thermostable soluble peroxidase from Citrus medica leaf. Prep Biochem Biotechnol. 2013;43:137-51 pubmed publisher
    ..The enzyme was purified 15.10-fold with a total yield of 28.6% by ammonium sulfate precipitation followed by Sephadex G-100 gel filtration chromatography...
  70. Yavuz M, Baysal Z. Preparation and use of poly(hydroxyethyl methacrylate) cryogels containing L-histidine for ?-casein adsorption. J Food Sci. 2013;78:E238-43 pubmed publisher
    ..17 mg/g cryogel. It was also observed that ?-casein could be repeatedly adsorbed and desorbed with p(HEMA-MAH) cryogel without significant loss in the adsorption capacity...
  71. Hou J, Tobe B, Lo F, Blethrow J, Crain A, Wolf D, et al. Combined total proteomic and phosphoproteomic analysis of human pluripotent stem cells. Methods Mol Biol. 2013;1029:163-89 pubmed publisher
    ..We summarize additional data analysis procedures, the subject of ongoing efforts...
  72. Dumetz A, Snellinger O brien A, Kaler E, Lenhoff A. Patterns of protein protein interactions in salt solutions and implications for protein crystallization. Protein Sci. 2007;16:1867-77 pubmed
    ..protein interactions generally show little salt dependence up to very high salt concentrations, whereas in ammonium sulfate, proteins show a sharp drop in b(2) with increasing salt concentration beyond a particular threshold...
  73. Ngo T, Narinesingh D. Kosmotropes enhance the yield of antibody purified by affinity chromatography using immobilized bacterial immunoglobulin binding proteins. J Immunoassay Immunochem. 2008;29:105-15 pubmed
    ..Thus, it appears that strongly hydrated anions (citrate, sulfate, and phosphate) and weakly hydrated cations (ammonium, potassium) increase the yield of antibody purified on either Protein A or Protein G affinity gels...
  74. Ghosalkar A, Sahai V, Srivastava A. Optimization of chemically defined medium for recombinant Pichia pastoris for biomass production. Bioresour Technol. 2008;99:7906-10 pubmed publisher
    ..This supports the notion that P. pastoris cells grow on glycerol by a respiratory route and are therefore an efficient biomass and protein producers...
  75. Munzi S, Loppi S, Cruz C, Branquinho C. Do lichens have "memory" of their native nitrogen environment?. Planta. 2011;233:333-42 pubmed publisher
    ..13,000 t/year) and those previously grown in an unpolluted area (nitrogen emissions of ca. 500 t/year). Greater N availability seems to enable X. parietina to cope better with the effects of nitrogen pollution...
  76. Sousa A, Bicho D, Tomaz C, Sousa F, Queiroz J. Performance of a non-grafted monolithic support for purification of supercoiled plasmid DNA. J Chromatogr A. 2011;1218:1701-6 pubmed publisher
    ..In fact, this new monolithic support arises as a powerful instrument on the sc pDNA purification for further clinical applications...
  77. Doty A, Buhi W, Benson S, Scoggin K, Pozor M, MacPherson M, et al. Equine CRISP3 modulates interaction between spermatozoa and polymorphonuclear neutrophils. Biol Reprod. 2011;85:157-64 pubmed publisher
    ..Seminal plasma was pooled from five stallions, and proteins were precipitated in 60% (w/v) ammonium sulfate and dialyzed (3500 MW cutoff)...
  78. Cody V, Pace J, Nowak J. Structural analysis of human dihydrofolate reductase as a binary complex with the potent and selective inhibitor 2,4-diamino-6-{2'-O-(3-carboxypropyl)oxydibenz[b,f]-azepin-5-yl}methylpteridine reveals an unusual binding mode. Acta Crystallogr D Biol Crystallogr. 2011;67:875-80 pubmed publisher
    ..The crystallization conditions differed for the human and mouse DHFR crystals (100?mM K2HPO4 pH 6.9, 30% ammonium sulfate for hDHFR; 15?mM Tris pH 8.3, 75?mM sodium cacodylate, PEG 4K for mDHFR)...
  79. Gao Q, Wang Y, He D, Ju C, Gao L, Fu M. Synthesis and conductive properties of polypyrrole nanocomposites. J Nanosci Nanotechnol. 2011;11:9836-9 pubmed
    ..Then, the solubility of polypyrrole was studied by doping with sodium dodecyl benzene sulfonate (SDBS), polyethylene glycol (PEG), and poly(styrene sulfonate) (PSS), proceeding the above-mentioned characterization...
  80. Zhu X, Burger M, Doane T, Horwath W. Ammonia oxidation pathways and nitrifier denitrification are significant sources of N2O and NO under low oxygen availability. Proc Natl Acad Sci U S A. 2013;110:6328-33 pubmed publisher
    ..5%, and 0% (vol/vol) O2 concentrations following urea or ammonium sulfate [(NH4)2SO4] additions in loam, clay loam, and sandy loam soils that also contained ample nitrate...
  81. Rizvi S, Banu N. Physicochemical properties and oxidative inactivation of soluble lectin from water buffalo (Bubalus bubalis) brain. Neurochem Res. 2008;33:468-76 pubmed
    ..The data from above study suggests that the isolated lectin may belong to the galectin-1 family but is glycosylated unlike those purified till date...
  82. Muturi E, Jacob B, Shililu J, Novak R. Laboratory studies on the effect of inorganic fertilizers on survival and development of immature Culex quinquefasciatus (Diptera: Culicidae). J Vector Borne Dis. 2007;44:259-65 pubmed
    Studies were conducted to determine the effect of ammonium sulfate (AM) and muriate of potash (MOP) fertilizers on survival and development of immature stages of Culex quinquefasciatus Say, a major vector of Bancroftian filariasis in ..
  83. Taupin P. A simple and direct pre-embedding technique for ultrastructure of scarce biological specimens. Biotech Histochem. 2008;83:253-7 pubmed publisher
    ..The ability to pre-embed scarce biological specimens efficiently and reproducibly provides a valuable way to study and characterize cytological tissues such as biopsies or cystic and amniotic fluid cells...
  84. Wang X, Maroto Valer M. Integration of CO2 capture and mineral carbonation by using recyclable ammonium salts. ChemSusChem. 2011;4:1291-300 pubmed publisher
    ..The mass balance of the process shows that about 2.63 tonnes of serpentine, 0.12 tonnes of NH(4)HSO(4), 7.48 tonnes of NH(4)HCO(3), and 0.04 tonnes of NH(3) are required to sequester 1 tonne of CO(2) as hydromagnesite...
  85. Wen C, Xiang X, Zeng W, Wu T, Tian C. [Extract berberine hydrochloride by aqueous two-phase system]. Zhong Yao Cai. 2011;34:1608-11 pubmed
    ..To study the aqueous two-phase system which could be used for extraction of berberine hydrochloride...
  86. Cernigoj U, Vidic U, Barut M, Podgornik A, Peterka M, Strancar A. A multimodal histamine ligand for chromatographic purification of plasmid DNA. J Chromatogr A. 2013;1281:87-93 pubmed publisher
    ..Furthermore, an efficient plasmid DNA separation was possible both on analytical as well as on preparative scale by applying the descending pH gradient at a constant concentration (above 3.0 M) of (NH(4))(2)SO(4)...
  87. Inada A, Oshima T, Baba Y. Enhancing the water dispersibility of paclitaxel by complexation with hydrophobic peptides. Colloids Surf B Biointerfaces. 2015;135:408-15 pubmed publisher
    ..Pep was prepared by enzymatic hydrolysis of casein, followed by fractionation using ammonium sulfate precipitation and ultrafiltration...
  88. Neppolian B, Celik E, Choi H. Photochemical oxidation of arsenic(III) to arsenic(V) using peroxydisulfate ions as an oxidizing agent. Environ Sci Technol. 2008;42:6179-84 pubmed
    ..Natural solar light could also effectively oxidize As(III) at room temperature. This simple technique was, thus, considered a cost-effective and safe method for the oxidation of As(III) to As(V)...
  89. Mandal A, Kar S, Das Mohapatra P, Maity C, Pati B, Mondal K. Purification and characterization of an endoxylanase from the culture broth of Bacillus cereus BSA1. Prikl Biokhim Mikrobiol. 2011;47:277-82 pubmed
    ..Since the enzyme was active over wide range ofpH, temperature and remained active in higher salt concentration, it could find potential uses in biobleaching process in paper industries...
  90. Josic D, Breen L, Clifton J, Gajdosik M, Gaso Sokac D, Rucevic M, et al. Separation of proteins from human plasma by sample displacement chromatography in hydrophobic interaction mode. Electrophoresis. 2012;33:1842-9 pubmed publisher
    ..Further low-abundance, physiologically active proteins could be highly enriched and detected by ESI-MS/MS...
  91. Rao D, Vishweshwaraiah Y, Gowda L. The enzymatic lectin of field bean (Dolichos lablab): salt assisted lectin-sugar interaction. Phytochemistry. 2012;83:7-14 pubmed publisher
    ..The salt concentrations required for Gal/GalNAc binding are not physiologically relevant in vivo, suggesting that the role of DLL-II per se in the seed is primarily that of a PPO purportedly for plant defense...
  92. Werner A, Hasse H. Experimental study and modeling of the influence of mixed electrolytes on adsorption of macromolecules on a hydrophobic resin. J Chromatogr A. 2013;1315:135-44 pubmed publisher
    ..The studied salts are sodium chloride, ammonium sulfate, sodium sulfate and ammonium chloride...