phosphorus radioisotopes

Summary

Summary: Unstable isotopes of phosphorus that decay or disintegrate emitting radiation. P atoms with atomic weights 28-34 except 31 are radioactive phosphorus isotopes.

Top Publications

  1. Pall G, Hamilton A. Improved northern blot method for enhanced detection of small RNA. Nat Protoc. 2008;3:1077-84 pubmed publisher
    ..Although chemical cross-linking takes longer (15 min to 2 h) than UV cross-linking, improved sensitivity means shorter periods of exposure are required to detect signal after hybridization...
  2. Abraham J, Sato F, Cheng Y, Paun B, Kan T, Olaru A, et al. Novel decapeptides that bind avidly and deliver radioisotope to colon cancer cells. PLoS ONE. 2007;2:e964 pubmed
    ..The rapidly growing field of targeted tumor therapy often utilizes an antibody, sometimes tagged with a tumor-ablating material such as radioisotope, directed against a specific molecule...
  3. Bettendorff L, Nghiêm H, Wins P, Lakaye B. A general method for the chemical synthesis of gamma-32P-labeled or unlabeled nucleoside 5(')-triphosphates and thiamine triphosphate. Anal Biochem. 2003;322:190-7 pubmed
    ..Our method can easily be scaled up for industrial synthesis of a variety of labeled and unlabeled triphosphoric derivatives from their mono- or diphosphate precursors...
  4. Rubio G, Sorgonà A, Lynch J. Spatial mapping of phosphorus influx in bean root systems using digital autoradiography. J Exp Bot. 2004;55:2269-80 pubmed
    ..However, a heterogeneous pattern was detected for basal roots when measured on a length basis, indicating that both root class and diameter constitute main factors controlling the spatial pattern of net influx...
  5. Shah N, Oros Peusquens A, Arrubla J, Zhang K, Warbrick T, Mauler J, et al. Advances in multimodal neuroimaging: hybrid MR-PET and MR-PET-EEG at 3 T and 9.4 T. J Magn Reson. 2013;229:101-15 pubmed publisher
  6. Funk D, Sorg B, Lindner S, Schmeiser H. 32P-postlabeling analysis of DNA adducts formed by leukotriene A4 (LTA4). Environ Mol Mutagen. 2010;51:338-43 pubmed publisher
    ..In summary, the TLC (32)P-postlabeling method is suitable for studying DNA adduct formation by LTA(4) and can be used for further investigations on the link between inflammation and cancer...
  7. Zhao R, Deng J, Liang X, Zeng J, Chen X, Wang J. Treatment of cystic craniopharyngioma with phosphorus-32 intracavitary irradiation. Childs Nerv Syst. 2010;26:669-74 pubmed publisher
    ..The aim of the study was to evaluate the effect of phosphorus-32 colloid ([(32)P]) intracavitary irradiation on the treatment of patients with cystic craniopharyngiomas...
  8. Phillips D, Arlt V. The 32P-postlabeling assay for DNA adducts. Nat Protoc. 2007;2:2772-81 pubmed
    ..It has a wide range of applications in human, animal and in vitro studies, and can be used for a wide variety of classes of compound and for the detection of adducts formed by complex mixtures. This protocol can be completed in 3 d...
  9. Yang C, Chen Y, Tsai H, Huang C, Huang T, Chen C. In vitro deoxynucleotidylation of the terminal protein of Streptomyces linear chromosomes. Appl Environ Microbiol. 2006;72:7959-61 pubmed
    ..We devised an in vitro system that specifically incorporated dCMP, the first nucleotide at the 5' ends, onto a threonine residue of the TP of Streptomyces coelicolor...

More Information

Publications62

  1. Ortolani P, Marzocchi A, Aquilina M, Gaiba W, Neri S, Bunkheila F, et al. Long-term clinical and angiographic outcome of patients with occlusive in-stent restenosis treated with (32P) beta-brachytherapy. Catheter Cardiovasc Interv. 2004;63:433-8 pubmed
    ..Further studies should determine the most suitable source and dosage of brachytherapy for patients with occlusive ISR...
  2. Yang Y, Lorick K, Jensen J, Weissman A. Expression and evaluation of RING finger proteins. Methods Enzymol. 2005;398:103-12 pubmed
    ..Use of these methods may help identify new E3s, dissect factors involved in ubiquitylation of substrates, and screen for molecules that affect ubiquitylation...
  3. Corazza A, Vianello F, Rigo A, Cadrobbi R, Baldan N, Rigotti P, et al. Biphasic behavior of the kinetics of 31P-containing metabolites in ischemic porcine kidneys. Transplant Proc. 2003;35:3111-5 pubmed
  4. Moghaddam M, Yang W, Bojarski B, Gengenbach T, Gao M, Zareie H, et al. Azide photochemistry for facile modification of graphitic surfaces: preparation of DNA-coated carbon nanotubes for biosensing. Nanotechnology. 2012;23:425503 pubmed publisher
  5. Guszczynski T, Specht S, Copeland T. A microtiter plate fraction collector for the sequencing of radioactive phosphorylated peptides. Anal Biochem. 2006;356:151-3 pubmed
  6. Mani R, Waring A, Lehrer R, Hong M. Membrane-disruptive abilities of beta-hairpin antimicrobial peptides correlate with conformation and activity: a 31P and 1H NMR study. Biochim Biophys Acta. 2005;1716:11-8 pubmed
  7. Osborne M, Arlt V, Kliem C, Hull W, Mirza A, Bieler C, et al. Synthesis, characterization, and 32p-postlabeling analysis of DNA adducts derived from the environmental contaminant 3-nitrobenzanthrone. Chem Res Toxicol. 2005;18:1056-70 pubmed
    ..Therefore, a different approach to the synthesis of authentic standards is needed for the structural characterization of 3-NBA-derived DNA adducts formed in vivo...
  8. Syeda B, Yahya N, Schmid R, Kirisits C, Denk S, Derntl M, et al. Increased dosage during intracoronary irradiation due to overlapped source stepping shows no long-term adverse changes in vessel morphology. J Interv Cardiol. 2004;17:143-9 pubmed
    ..The purpose of this analysis was to evaluate if overdosage during intracoronary irradiation due to overlapped source stepping may result in long-term morphologic changes in vessel anatomy...
  9. Lomas Lopez R, Paracuellos P, Riberty M, Cozzone A, Duclos B. Several enzymes of the central metabolism are phosphorylated in Staphylococcus aureus. FEMS Microbiol Lett. 2007;272:35-42 pubmed
    ..The identification of these phosphoproteins should prove helpful in understanding and unravelling of the role of phosphorylation with respect to pathogenesis and virulence in this organism...
  10. Lin L, Wan F, Hu J. Functional and structural dynamics of hepadnavirus reverse transcriptase during protein-primed initiation of reverse transcription: effects of metal ions. J Virol. 2008;82:5703-14 pubmed publisher
  11. Hohenegger M, Freissmuth M, Nanoff C. Covalent modification of G-proteins by affinity labeling. Methods Mol Biol. 2004;259:183-95 pubmed
    ..One of the compounds we present here (2',3'-dialdehyde-GTP) can also be employed to block receptor-mediated G-protein activation and to disrupt the cognate signaling pathway...
  12. Siow D, Wattenberg B. An assay system for measuring the acute production of sphingosine 1-phosphate in intact monolayers. Anal Biochem. 2007;371:184-93 pubmed
    ..Overall, this assay is ideal for future studies to identify changes in S1P production in intact cells such as those that result from the differential intracellular targeting of sphingosine kinase...
  13. Ortolani P, Marzocchi A, Aquilina M, Gaiba W, Neri S, Marrozzini C, et al. 32P brachytherapy in the treatment of complex Cypher in-stent restenosis. J Interv Cardiol. 2005;18:205-11 pubmed
    ..This finding should encourage systematic studies on the safety and efficacy of IRT in this problematic setting...
  14. Russo Abrahão T, Alves Bezerra M, Majerowicz D, Freitas Mesquita A, Dick C, Gondim K, et al. Transport of inorganic phosphate in Leishmania infantum and compensatory regulation at low inorganic phosphate concentration. Biochim Biophys Acta. 2013;1830:2683-9 pubmed
    ..Proliferation of Leishmania infantum depends on exogenous inorganic phosphate (P(i)) but little is known about energy metabolism and transport of P(i) across the plasma membrane in Leishmania sp...
  15. Dong H, Suzuki N, Torres M, Bonala R, Johnson F, Grollman A, et al. Quantitative determination of aristolochic acid-derived DNA adducts in rats using 32P-postlabeling/polyacrylamide gel electrophoresis analysis. Drug Metab Dispos. 2006;34:1122-7 pubmed
    ..This study establishes the feasibility of using AA-DNA adducts as intermediate biomarkers of exposure in studies of AA nephropathy and its associated urothelial cancer...
  16. Jia Y, Quinn C, Clabbers A, Talanian R, Xu Y, Wishart N, et al. Comparative analysis of various in vitro COT kinase assay formats and their applications in inhibitor identification and characterization. Anal Biochem. 2006;350:268-76 pubmed
    ..The knowledge gained through our studies with COT, and the methods described for our assays and compound mechanistic studies, can be readily applied to other kinase targets...
  17. Pimkin M, Markham G. The CBS subdomain of inosine 5'-monophosphate dehydrogenase regulates purine nucleotide turnover. Mol Microbiol. 2008;68:342-59 pubmed publisher
    ..We conclude that the CBS subdomain of IMPDH may coordinate the activities of the enzymes of purine nucleotide metabolism and is essential for maintaining the normal ATP and GTP pool sizes in E. coli...
  18. Voytas D, Ke N. Detection and quantitation of radiolabeled proteins and DNA in gels and blots. Curr Protoc Mol Biol. 2001;Appendix 3:Appendix 3A pubmed publisher
    ..Sensitivity and linear ranges of measurement can also be greatly extended by using a phosphor imaging system to quickly and easily quantify radioactive samples...
  19. Hassel M, Cornelius M, Vom Brocke J, Schmeiser H. Total nucleotide analysis of Hydra DNA and RNA by MEKC with LIF detection and 32P-postlabeling. Electrophoresis. 2010;31:299-302 pubmed publisher
    ..6 and 3.1%. Total Hydra RNA showed highest base levels for guanine (33%) and a level of 1.4% for pseudouracil. All values were in good agreement with those determined by the (32)P-postlabeling method...
  20. Nair J, Nair U, Sun X, Wang Y, Arab K, Bartsch H. Quantifying etheno-DNA adducts in human tissues, white blood cells, and urine by ultrasensitive (32)P-postlabeling and immunohistochemistry. Methods Mol Biol. 2011;682:189-205 pubmed publisher
    ..The described methods are useful in diagnosis and monitoring of chronic degenerative diseases, including cancer, atherosclerosis, and neurodegenerative disorders...
  21. Ochiai M, Sugimura T, Nagao M. Modification of the 32P-postlabeling method to detect a single adduct species as a single spot. Methods Mol Biol. 2005;291:13-9 pubmed
    ..The advantage of these methods is that the number of adduct species formed can be estimated by TLC...
  22. Hasegawa T, Kondziolka D, Hadjipanayis C, Lunsford L. Management of cystic craniopharyngiomas with phosphorus-32 intracavitary irradiation. Neurosurgery. 2004;54:813-20; discussion 820-2 pubmed
    ..Limited data are available regarding long-term outcomes...
  23. Frosina G, Cappelli E, Ropolo M, Fortini P, Pascucci B, Dogliotti E. In vitro base excision repair assay using mammalian cell extracts. Methods Mol Biol. 2006;314:377-96 pubmed
    ..The construction of a single lesion at a defined site of the plasmid genome makes the substrate amenable to fine mapping of the repair patches, thus allowing discrimination between the two BER pathways...
  24. Walker J, Walker L, Margulies K, Buttrick P, de Tombe P. Protein kinase A changes calcium sensitivity but not crossbridge kinetics in human cardiac myofibrils. Am J Physiol Heart Circ Physiol. 2011;301:H138-46 pubmed publisher
  25. Watkins H, Goldrick M. Detection of mutations by RNase cleavage. Curr Protoc Hum Genet. 2001;Chapter 7:Unit 7.2 pubmed publisher
    ..The unit also discusses the use of alternative ribonucleases for cleaving singlebase mismatches...
  26. Mardis E. Next-generation sequencing platforms. Annu Rev Anal Chem (Palo Alto Calif). 2013;6:287-303 pubmed publisher
  27. Perrone C, Ahr H, Duan J, Jeffrey A, Schmidt U, Williams G, et al. Embryonic turkey liver: activities of biotransformation enzymes and activation of DNA-reactive carcinogens. Arch Toxicol. 2004;78:589-98 pubmed
    ..Thus, turkey embryos could be utilized to investigate potential chemical toxicity and carcinogenicity...
  28. Jones N. ³²P-postlabelling for the sensitive detection of DNA adducts. Methods Mol Biol. 2012;817:183-206 pubmed publisher
    ..For these reasons, the (32)-postlabelling assay represents an extremely versatile and extremely sensitive method to detect and monitor DNA damage...
  29. Medeiros M. Exocyclic DNA adducts as biomarkers of lipid oxidation and predictors of disease. Challenges in developing sensitive and specific methods for clinical studies. Chem Res Toxicol. 2009;22:419-25 pubmed publisher
    ..This review focuses on recent developments in the use of these adducts as possible biomarkers for disease risk related to oxidative stress and on the challenges in developing sensitive and specific methods for clinical studies...
  30. Wei J, Davis K, Wu H, Wu J. Protein phosphorylation of human brain glutamic acid decarboxylase (GAD)65 and GAD67 and its physiological implications. Biochemistry. 2004;43:6182-9 pubmed
    ..Furthermore, mutation of T91 to aspartic acid or glutamic acid mimics the effect of phosphorylation. A model depicting the effect of phosphorylation on GAD activity upon neuronal stimulation is also proposed...
  31. Salgueiro M, Duran H, Palmieri M, Pirchio R, Medina V, Ughetti R, et al. Bioevaluation of 32P patch designed for the treatment of skin diseases. Nucl Med Biol. 2008;35:233-7 pubmed publisher
    ..The objective of this study was to design and evaluate a 32P patch for the treatment of skin diseases...
  32. Carman G, Wu W. Lipid phosphate phosphatases from Saccharomyces cerevisiae. Methods Enzymol. 2007;434:305-15 pubmed
    ..The properties of the DPP1-encoded and LPP1-encoded lipid phosphate phosphatase enzymes are summarized...
  33. Li Y, Schaffer P, Perrin D. Dual isotope labeling: conjugation of 32P-oligonucleotides with 18F-aryltrifluoroborate via copper(I) catalyzed cycloaddition. Bioorg Med Chem Lett. 2013;23:6313-6 pubmed publisher
    ..These results are corroborated by HPLC analysis of the labeled material. Taken together this work demonstrates viable use of (18)F-ArBF3(-) prosthetics for labeling oligonucleotides for use in PET imaging...
  34. Hwang B, Katner J, Iyengar S. Corticotropin-releasing factor mRNA and substance P receptor binding in the paraventricular hypothalamic nucleus, central nucleus of the amygdala, and locus coeruleus of Sprague-Dawley rats following restraint-induced stress. J Mol Neurosci. 2005;25:239-50 pubmed
    ..In concert with the literature indicating SP antagonist's antidepressive effects, up-regulated SP receptors in the CeA might contribute to the development of stress-related depression...
  35. Thompson T, Certain M. Estrogen mediated inhibition of dopamine transport in the striatum: regulation by G alpha i/o. Eur J Pharmacol. 2005;511:121-6 pubmed
    ..These data are consistent with an estrogen mediated alteration of G-protein activity and support the hypothesis that estrogen may alter transporter activity through a modulation of dopamine D2 autoreceptor/G alpha(i/o) protein coupling...
  36. Drachkova I, Lysova M, Repkova M, Prokuda O, Sokolenko A, Arshinova T, et al. [Interaction of proteins from general transcription complex RNA polymerase II with oligoribonucleotides]. Mol Biol (Mosk). 2005;39:139-46 pubmed
    ..The data presented demonstrate that oligoribonucleotide and oligodeoxyribonucleotide don't compete for the binding sites on a basal transcription complex proteins...
  37. Kovacs P, Csaba G. The effect of histone deacetylase inhibitor trichostatin A (TSA) on the incorporation of 32P (Pi) and 3H-palmitic acid into the phospholipids of Tetrahymena. Cell Biochem Funct. 2008;26:39-42 pubmed
    ..32P incorporation was also inhibited. The possible relation between the microtubular system and signalling is discussed...
  38. Munnia A, Saletta F, Allione A, Piro S, Confortini M, Matullo G, et al. 32P-Post-labelling method improvements for aromatic compound-related molecular epidemiology studies. Mutagenesis. 2007;22:381-5 pubmed
    ..The above observations indicate that DNA adducts being measured are likely to have been induced by aromatic compounds...
  39. Dal Cin V, Galla G, Ramina A. MdACO expression during abscission: the use of 33P labeled primers in transcript quantitation. Mol Biotechnol. 2007;36:9-13 pubmed
    ..The use of either the reverse primer or the anchored oligo dT13 produced clear and elegant results. Nevertheless, the initial quantity of labeled primer appeared to be a crucial factor for obtaining a wide range titration curve...
  40. Mahieu S, Navoni J, Millen N, Del Carmen Contini M, Gonzalez M, Elías M. Effects of aluminum on phosphate metabolism in rats: a possible interaction with vitamin D3 renal production. Arch Toxicol. 2004;78:609-16 pubmed
    ..These findings suggest that Al could modify the Pi metabolism acting directly on intestine, kidney and bone, or indirectly through possible changes in the levels of vitamin D3...
  41. Abu Ghazalah R, Macgregor R. Structural polymorphism of the four-repeat Oxytricha nova telomeric DNA sequences. Biophys Chem. 2009;141:180-5 pubmed publisher
    ..The results presented here are an addition to the modest information available for predicting the type of G-quadruplex to be formed from G-rich sequences in aqueous solutions containing sodium or potassium ions...
  42. Hua G, Lyons B, Killham K, Singleton I. Potential use of DNA adducts to detect mutagenic compounds in soil. Environ Pollut. 2009;157:916-21 pubmed publisher
    ..However, this new method provides a potential way to detect mutagenic compounds in contaminated soil and to assess the outcomes of soil remediation...
  43. Hale J, Doherty G, Toth L, Mills S, Hajdu R, Keohane C, et al. Selecting against S1P3 enhances the acute cardiovascular tolerability of 3-(N-benzyl)aminopropylphosphonic acid S1P receptor agonists. Bioorg Med Chem Lett. 2004;14:3501-5 pubmed
  44. Malmstrom C, Konn M, Bogovski S, Lang T, Lönnström L, Bylund G. Screening of hydrophobic DNA adducts in flounder (Platichthys flesus) from the Baltic Sea. Chemosphere. 2009;77:1514-9 pubmed publisher
    ..Our results indicate that flounder from studied off shore sites of the Baltic Sea had not been exposed to a greater extent to large polycyclic hydrophobic hydrocarbons in their environment...
  45. Liepe K, Zaknun J, Padhy A, Barrenechea E, Soroa V, Shrikant S, et al. Radiosynovectomy using yttrium-90, phosphorus-32 or rhenium-188 radiocolloids versus corticoid instillation for rheumatoid arthritis of the knee. Ann Nucl Med. 2011;25:317-23 pubmed publisher
    ..Radiocolloids labelled with less costly and more accessible radionuclides are of interest to developing countries. We investigated the efficacy of different formulations in RA...
  46. Demian D, Clugston S, Foster M, Rameh L, Sarkes D, Townson S, et al. High-throughput, cell-free, liposome-based approach for assessing in vitro activity of lipid kinases. J Biomol Screen. 2009;14:838-44 pubmed publisher
    ..This assay system can be applied to other types and isoforms of PIPKs as well as a variety of other lipid kinase targets...
  47. Kameshita I. In-gel phosphatase assay using fluorogenic and radioactive substrates. Curr Protoc Protein Sci. 2011;Chapter 13:Unit13.12 pubmed publisher
    ..Each method has advantages and disadvantages that are discussed in the commentary...
  48. Sadeghi M, Karimi E, Sardari D. Monte Carlo and analytical calculations of dose distributions in craniopharyngioma cysts treated with radiocolloids containing 32P or 186Re. Appl Radiat Isot. 2009;67:1697-701 pubmed publisher
    ..The effects of the cyst wall type and of the density of the cyst inner fluid were investigated. The (32)P activities required for providing 200, 250, and 300 Gy to cysts of different sizes were calculated...
  49. Venkatesh L, Fasina O, Pintel D. RNAse mapping and quantitation of RNA isoforms. Methods Mol Biol. 2012;883:121-9 pubmed publisher
    ..RPAs are highly sensitive, the probes can be specifically targeted, and, when performed in probe excess, are quantitative, making them the method of choice for many analyses of RNA processing events. ..
  50. Leupold J, Wieben O, Mansson S, Speck O, Scheffler K, Petersson J, et al. Fast chemical shift mapping with multiecho balanced SSFP. MAGMA. 2006;19:267-73 pubmed
    ..A method is proposed that provides spectroscopic images with high spatial resolution and moderate spectral resolution at very short total data acquisition times...
  51. Pines J, Jackman M, Simpson K. Assays for CDK activity and DNA replication in the cell cycle. Curr Protoc Cell Biol. 2001;Chapter 8:Unit 8.2 pubmed publisher
    ..These assays are useful in analyzing the stage and degree of synchrony of the cell cycle and changes in the basic cell cycle machinery...
  52. Li A, Guan K, Stonor R, Smith S, Smith F. Direct and indirect influences of arbuscular mycorrhizal fungi on phosphorus uptake by two root hemiparasitic Pedicularis species: do the fungal partners matter at low colonization levels?. Ann Bot. 2013;112:1089-98 pubmed publisher
    ..The aim of this study was to test the influence of AM inoculation on phosphorus (P) uptake by Pedicularis rex and P. tricolor...
  53. Andréasson B, Lofvenberg E, Westin J. Management of patients with polycythaemia vera: results of a survey among Swedish haematologists. Eur J Haematol. 2005;74:489-95 pubmed
    ..The use of different therapeutic modalities is very variable. Many physicians carry out their phlebotomy treatment with less intensity compared with national and international recommendations...