nitrogen isotopes

Summary

Summary: Stable nitrogen atoms that have the same atomic number as the element nitrogen, but differ in atomic weight. N-15 is a stable nitrogen isotope.

Top Publications

  1. Madigan D, Litvin S, Popp B, Carlisle A, Farwell C, Block B. Tissue turnover rates and isotopic trophic discrimination factors in the endothermic teleost, pacific bluefin tuna (Thunnus orientalis). PLoS ONE. 2012;7:e49220 pubmed publisher
    ..These results allow more accurate interpretation of field data and dramatically improve our ability to use stable isotope data from wild tunas to better understand their migration patterns and trophic ecology. ..
  2. Robbins C, Felicetti L, Florin S. The impact of protein quality on stable nitrogen isotope ratio discrimination and assimilated diet estimation. Oecologia. 2010;162:571-9 pubmed publisher
    ..We conclude that using isotopes to estimate assimilated diets is more complex than often appreciated and will require developing more biologically based, time-sensitive models...
  3. Mohr W, Grosskopf T, Wallace D, Laroche J. Methodological underestimation of oceanic nitrogen fixation rates. PLoS ONE. 2010;5:e12583 pubmed publisher
    ..We hypothesise that application of N(2) fixation measurements using this modified method will significantly reduce the apparent imbalances in the oceanic fixed-nitrogen budget...
  4. Wang J, Zhang Y, Li S, Liu X, Yan X, Song H, et al. Overproduction of 15N-labeled r-RGD-hirudin in pichia pastoris for NMR studies. Protein Pept Lett. 2010;17:1228-33 pubmed
    ..Heteronuclear NMR of the (15)N-labeled r-RGD-hirudin was performed for the first time, and all signals in the heteronuclear single quantum coherence (HSQC) spectrum were successfully assigned...
  5. Dayie T, Thakur C. Site-specific labeling of nucleotides for making RNA for high resolution NMR studies using an E. coli strain disabled in the oxidative pentose phosphate pathway. J Biomol NMR. 2010;47:19-31 pubmed publisher
  6. Jones D, Cellitti S, Hao X, Zhang Q, Jahnz M, Summerer D, et al. Site-specific labeling of proteins with NMR-active unnatural amino acids. J Biomol NMR. 2010;46:89-100 pubmed publisher
    ..In this perspective, we discuss these opportunities including new photocaged unnatural amino acids, outline usage of metal chelating and spin-labeled unnatural amino acids and expand the approach to in-cell NMR experiments...
  7. Bond A, Diamond A. Recent Bayesian stable-isotope mixing models are highly sensitive to variation in discrimination factors. Ecol Appl. 2011;21:1017-23 pubmed
  8. Santoro A, Buchwald C, McIlvin M, Casciotti K. Isotopic signature of N(2)O produced by marine ammonia-oxidizing archaea. Science. 2011;333:1282-5 pubmed publisher
    ..Our results suggest that ammonia-oxidizing archaea may be largely responsible for the oceanic N(2)O source...
  9. Fromentin C, Sanders P, Nau F, Anton M, Fromentin G, Tome D, et al. A pilot study for the intrinsic labeling of egg proteins with 15N and 13C. Rapid Commun Mass Spectrom. 2012;26:43-8 pubmed publisher
    ..However, due to the non-homogeneity of the (13)C labeling, adequate (13)C enrichment of individual amino acids must be considered depending on the investigated metabolic pathway...

More Information

Publications62

  1. Hamer G, Donovan D, Hood Nowotny R, Kaufman M, Goldberg T, Walker E. Evaluation of a stable isotope method to mark naturally-breeding larval mosquitoes for adult dispersal studies. J Med Entomol. 2012;49:61-70 pubmed
    ..This stable isotope marking technique will facilitate studies of mosquito dispersal...
  2. McAlister G, Huttlin E, Haas W, Ting L, Jedrychowski M, Rogers J, et al. Increasing the multiplexing capacity of TMTs using reporter ion isotopologues with isobaric masses. Anal Chem. 2012;84:7469-78 pubmed publisher
    ..Considering the structure of the TMT reporter ion, we believe this work serves as a blueprint for expanding the multiplexing capacity of the TMT reagents to at least 10-plex and possibly up to 18-plex...
  3. Sauheitl L, Glaser B, Weigelt A. Advantages of compound-specific stable isotope measurements over bulk measurements in studies on plant uptake of intact amino acids. Rapid Commun Mass Spectrom. 2009;23:3333-42 pubmed publisher
    ..We therefore highly recommend the use of CSI measurements for future amino acid uptake studies due to their higher accuracy...
  4. Diebel M, Vander Zanden M. Nitrogen stable isotopes in streams: effects of agricultural sources and transformations. Ecol Appl. 2009;19:1127-34 pubmed
    ..Based on our findings and on agricultural census data for the entire United States, inorganic fertilizer is more likely than manure to drive variability in biotic delta15N and to cause excessive nitrogen concentrations in streams...
  5. Murray I, Wolf B. Tissue carbon incorporation rates and diet-to-tissue discrimination in ectotherms: tortoises are really slow. Physiol Biochem Zool. 2012;85:96-105 pubmed publisher
    ..These results continue to highlight the importance of laboratory experiments in interpreting isotopic data derived from field studies...
  6. Sun S, Han Y, Paramasivam S, Yan S, Siglin A, Williams J, et al. Solid-state NMR spectroscopy of protein complexes. Methods Mol Biol. 2012;831:303-31 pubmed publisher
    ..We present protocols for sample preparation and characterization, pulse sequences, SSNMR spectra collection, and data analysis...
  7. Flores P, Murray P, Hellin P, Fenoll J. Influence of N doses and form on ¹?N natural abundance of pepper plants: considerations for using ?¹?N values as indicator of N source. J Sci Food Agric. 2011;91:2255-8 pubmed publisher
    ..To this end, different N concentrations (applied as NO(3) (-) ), N forms (NO(3) (-) versus NH(4) (+) ), and increasing NO(3) (-) applications to an organic N-based fertilization regime were studied...
  8. Falxa Raymond N, Patterson A, Schuster W, Griffin K. Oak loss increases foliar nitrogen, ?(15)N and growth rates of Betula lenta in a northern temperate deciduous forest. Tree Physiol. 2012;32:1092-101 pubmed publisher
    ..The loss of oaks and subsequent increase in black birch productivity may have a lasting impact on ecosystem form and function...
  9. Chen J, Wang J. A segmental labeling strategy for unambiguous determination of domain-domain interactions of large multi-domain proteins. J Biomol NMR. 2011;50:403-10 pubmed publisher
    ..This straightforward approach permits unambiguous identification of 78 inter-domain NOEs, enabling accurate definition of the relative positions of both the NT- and the CT-domains and determination of the NMR structure of apoE...
  10. Sturm M, Lojen S. Nitrogen isotopic signature of vegetables from the Slovenian market and its suitability as an indicator of organic production. Isotopes Environ Health Stud. 2011;47:214-20 pubmed publisher
    ..The results obtained indicate that the method has several limitations. Hence, ?(15)N could be a reliable indicator for organic production, but nevertheless, the conclusion has to be validated by background information...
  11. Valenzuela L, Chesson L, O Grady S, Cerling T, Ehleringer J. Spatial distributions of carbon, nitrogen and sulfur isotope ratios in human hair across the central United States. Rapid Commun Mass Spectrom. 2011;25:861-8 pubmed publisher
    ..The accuracy of the map was tested using hair samples not included in its generation. We conclude that sulfur isotope analysis may represent a new tool to investigate the movements and/or region-of-origin of humans...
  12. Hobbie E, Högberg P. Nitrogen isotopes link mycorrhizal fungi and plants to nitrogen dynamics. New Phytol. 2012;196:367-382 pubmed publisher
  13. Williams L, White C, Longstaffe F. Improving stable isotopic interpretations made from human hair through reduction of growth cycle error. Am J Phys Anthropol. 2011;145:125-36 pubmed publisher
  14. Sturm M, Kacjan Maršić N, Lojen S. Can ?(15)N in lettuce tissues reveal the use of synthetic nitrogen fertiliser in organic production?. J Sci Food Agric. 2011;91:262-7 pubmed publisher
    ..treated with synthetic and organic nitrogen fertilisers (single or split application). The effect of combined usage of synthetic and organic fertilisers on ?(15)N was also investigated...
  15. Clode P, Kilburn M, Jones D, Stockdale E, Cliff J, Herrmann A, et al. In situ mapping of nutrient uptake in the rhizosphere using nanoscale secondary ion mass spectrometry. Plant Physiol. 2009;151:1751-7 pubmed publisher
    ..Nanoscale secondary ion mass spectrometry has potential to allow the study of assimilatory processes at the submicron level in a wide range of applications involving plants, microorganisms, and animals...
  16. Thakur C, Brown M, Sama J, Jackson M, Dayie T. Growth of wildtype and mutant E. coli strains in minimal media for optimal production of nucleic acids for preparing labeled nucleotides. Appl Microbiol Biotechnol. 2010;88:771-9 pubmed publisher
    ..These studies should benefit efforts to make selective 13C-15N isotopic-labeled nucleotides for synthesizing biologically important RNAs...
  17. Michalik A, McGill R, Furness R, Eggers T, van Noordwijk H, Quillfeldt P. Black and white--does melanin change the bulk carbon and nitrogen isotope values of feathers?. Rapid Commun Mass Spectrom. 2010;24:875-8 pubmed publisher
    ..Care should be taken in future stable isotope studies when comparing differently coloured feathers...
  18. Nakazawa T, Sakai Y, Hsieh C, Koitabashi T, Tayasu I, Yamamura N, et al. Is the relationship between body size and trophic niche position time-invariant in a predatory fish? First stable isotope evidence. PLoS ONE. 2010;5:e9120 pubmed publisher
    ..We proposed hypotheses for the underlying mechanism of the time-varying size-trophic relationship...
  19. Cuhel J, Simek M, Laughlin R, Bru D, Chèneby D, Watson C, et al. Insights into the effect of soil pH on N(2)O and N(2) emissions and denitrifier community size and activity. Appl Environ Microbiol. 2010;76:1870-8 pubmed publisher
    ..However, this highlights the need for further studies combining analysis of denitrifier ecology and quantification of denitrification end products for a comprehensive understanding of the regulation of N fluxes by denitrification...
  20. Cherel Y, Kernaleguen L, Richard P, Guinet C. Whisker isotopic signature depicts migration patterns and multi-year intra- and inter-individual foraging strategies in fur seals. Biol Lett. 2009;5:830-2 pubmed publisher
    ..The isotopic signature of whiskers, therefore, revealed new multi-year foraging strategies of male Antarctic fur seals and is a powerful tool for investigating the ecological niche during cryptic stages of mammals' life...
  21. Loiacono S, Meyer Dombard D, Havig J, Poret Peterson A, Hartnett H, Shock E. Evidence for high-temperature in situ nifH transcription in an alkaline hot spring of Lower Geyser Basin, Yellowstone National Park. Environ Microbiol. 2012;14:1272-83 pubmed publisher
    ..7°C) for nitrogen fixation in alkaline, terrestrial hydrothermal environments...
  22. Jackson M, Donohue I, Jackson A, Britton J, Harper D, Grey J. Population-level metrics of trophic structure based on stable isotopes and their application to invasion ecology. PLoS ONE. 2012;7:e31757 pubmed publisher
    ..Although we have demonstrated their applicability specifically using a detailed temporal dataset of species invasion in a lake, they have a wide array of potential ecological applications...
  23. Yousfi S, Serret M, Márquez A, Voltas J, Araus J. Combined use of ?¹³C, ?18O and ?15N tracks nitrogen metabolism and genotypic adaptation of durum wheat to salinity and water deficit. New Phytol. 2012;194:230-44 pubmed publisher
    ..Based upon these results, the key role of N metabolism in durum wheat adaptation to salinity and water stress is highlighted...
  24. Fouillet H, Juillet B, Gaudichon C, Mariotti F, Tome D, Bos C. Absorption kinetics are a key factor regulating postprandial protein metabolism in response to qualitative and quantitative variations in protein intake. Am J Physiol Regul Integr Comp Physiol. 2009;297:R1691-705 pubmed publisher
  25. Kotur Z, Mackenzie N, Ramesh S, Tyerman S, Kaiser B, Glass A. Nitrate transport capacity of the Arabidopsis thaliana NRT2 family members and their interactions with AtNAR2.1. New Phytol. 2012;194:724-31 pubmed publisher
    ..1. • Co-injection into Xenopus oocytes of cRNA of all NRT2 genes together with cRNA of AtNAR2.1 resulted in statistically significant increases of uptake over and above that resulting from single cRNA injections...
  26. Balster N, Marshall J, Clayton M. Coupling tree-ring delta13C and delta15N to test the effect of fertilization on mature Douglas-fir (Pseudotsuga menziesii var. glauca) stands across the Interior northwest, USA. Tree Physiol. 2009;29:1491-501 pubmed publisher
    ..76). These data lead us to suggest that alternative mechanisms underlie the growth response to fertilizer, i.e., increase in canopy area and shifts in biomass allocation...
  27. Huang T, Sun L, Stark J, Wang Y, Cheng Z, Yang Q, et al. Relative changes in krill abundance inferred from Antarctic fur seal. PLoS ONE. 2011;6:e27331 pubmed publisher
    ..This novel method makes it possible to infer past krill population changes from ancient tissues of krill predators...
  28. Polito M, Trivelpiece W, Karnovsky N, Ng E, Patterson W, Emslie S. Integrating stomach content and stable isotope analyses to quantify the diets of pygoscelid penguins. PLoS ONE. 2011;6:e26642 pubmed publisher
  29. Nitsch E, Humphrey L, Hedges R. Using stable isotope analysis to examine the effect of economic change on breastfeeding practices in Spitalfields, London, UK. Am J Phys Anthropol. 2011;146:619-28 pubmed publisher
    ..Not all individuals at Spitalfields were breastfed, and there may not have been a single uniformly practiced weaning scheme. There is, however, more evidence for prolonged breastfeeding during the 19th century than the 18th century...
  30. Warren C. Post-uptake metabolism affects quantification of amino acid uptake. New Phytol. 2012;193:522-31 pubmed publisher
    ..Alternatively, post-uptake metabolism and transport could be minimized by harvesting plants within minutes of isotope addition...
  31. Thompson A, Chesson L, Podlesak D, Bowen G, Cerling T, Ehleringer J. Stable isotope analysis of modern human hair collected from Asia (China, India, Mongolia, and Pakistan). Am J Phys Anthropol. 2010;141:440-51 pubmed publisher
    ..C, N, and S isotope ratios supported marine food consumption in some coastal locales. N isotope ratios suggested a relatively low consumption of animal-derived products compared to western populations...
  32. Lecomte N, Ahlstrøm O, Ehrich D, Fuglei E, Ims R, Yoccoz N. Intrapopulation variability shaping isotope discrimination and turnover: experimental evidence in arctic foxes. PLoS ONE. 2011;6:e21357 pubmed publisher
    ..Our experimental analysis provides a framework for quantifying multiple sources of variation in isotopic discrimination and half-life that needs to be taken into account when designing and analysing ecological field studies. ..
  33. Ariz I, Cruz C, Moran J, González Moro M, García Olaverri C, González Murua C, et al. Depletion of the heaviest stable N isotope is associated with NH4+/NH3 toxicity in NH4+-fed plants. BMC Plant Biol. 2011;11:83 pubmed publisher
    ..0 and 9.0 (which favours NH3 form) were carried out in order to support and substantiate our hypothesis. N source fractionation throughout the whole plant was interpreted on the basis of the relative transport of NH4+ and NH3...
  34. Jaeger A, Cherel Y. Isotopic investigation of contemporary and historic changes in penguin trophic niches and carrying capacity of the southern Indian ocean. PLoS ONE. 2011;6:e16484 pubmed publisher
  35. Cernusak L, Winter K, Turner B. Plant delta 15N correlates with the transpiration efficiency of nitrogen acquisition in tropical trees. Plant Physiol. 2009;151:1667-76 pubmed publisher
    ..These results provide a simple explanation of variation in delta(15)N of terrestrial plants and have implications for understanding nitrogen cycling in ecosystems...
  36. Kim S, Tinker M, Estes J, Koch P. Ontogenetic and among-individual variation in foraging strategies of northeast Pacific white sharks based on stable isotope analysis. PLoS ONE. 2012;7:e45068 pubmed publisher
    ..Our data reveal substantial ontogenetic and individual dietary variation within a white shark population. ..
  37. Smith C, Morgan M, Pilbeam D. Isotopic ecology and dietary profiles of Liberian chimpanzees. J Hum Evol. 2010;58:43-55 pubmed publisher
    ..Despite the high diversity of dietary items consumed by chimpanzees, isotopic signatures of chimpanzees from a primary rainforest setting exhibit narrow ranges of variation similar to chimpanzees in more open habitats...
  38. Kim S, del Rio C, Casper D, Koch P. Isotopic incorporation rates for shark tissues from a long-term captive feeding study. J Exp Biol. 2012;215:2495-500 pubmed publisher
    ..model with first-order kinetics adequately described the incorporation patterns of carbon and nitrogen isotopes for these three tissues...
  39. Wang M, Chen J, Turko I. 15N-labeled full-length apolipoprotein E4 as an internal standard for mass spectrometry quantification of apolipoprotein E isoforms. Anal Chem. 2012;84:8340-4 pubmed publisher
    ..Overall, using this method, it is possible to study the differences between apoE isoform functions on the protein level and therefore to understand the underlying biological mechanism by which apoE alters AD susceptibility...
  40. Martos F, Dulormne M, Pailler T, Bonfante P, Faccio A, Fournel J, et al. Independent recruitment of saprotrophic fungi as mycorrhizal partners by tropical achlorophyllous orchids. New Phytol. 2009;184:668-81 pubmed publisher
    ..By enlarging the spectrum of mycorrhizal fungi and the level of specificity in mycoheterotrophic orchids, this study provides new insights on orchid and mycorrhizal biology in the tropics...
  41. Newsome S, Bentall G, Tinker M, Oftedal O, Ralls K, Estes J, et al. Variation in delta13C and delta15N diet-vibrissae trophic discrimination factors in a wild population of California sea otters. Ecol Appl. 2010;20:1744-52 pubmed
  42. Dekas A, Poretsky R, Orphan V. Deep-sea archaea fix and share nitrogen in methane-consuming microbial consortia. Science. 2009;326:422-6 pubmed publisher
    ..This finding extends the demonstrated lower limits of respiratory energy capable of fueling N2 fixation and reveals a link between the global carbon, nitrogen, and sulfur cycles...
  43. Nash S, Bersamin A, Kristal A, Hopkins S, Church R, Pasker R, et al. Stable nitrogen and carbon isotope ratios indicate traditional and market food intake in an indigenous circumpolar population. J Nutr. 2012;142:84-90 pubmed publisher
    ..Self-report methods showed variations by age and cultural identity only. Thus, stable isotopes show potential as biomarkers for monitoring dietary change in indigenous circumpolar populations...
  44. Romero G, Nomura F, Gonçalves A, Dias N, Mercier H, Conforto E, et al. Nitrogen fluxes from treefrogs to tank epiphytic bromeliads: an isotopic and physiological approach. Oecologia. 2010;162:941-9 pubmed publisher
    ..Since amphibian-bromeliad associations occur in diverse habitats in South and Central America, this mechanism for deriving nutrients may be important in bromeliad systems throughout the Neotropics...
  45. Godfrey L, Glass J. The geochemical record of the ancient nitrogen cycle, nitrogen isotopes, and metal cofactors. Methods Enzymol. 2011;486:483-506 pubmed publisher
    ..This chapter reviews the enzymology and metal cofactors of the N cycle and describes proper utilization of methods used to reconstruct evolution of the N cycle through time...
  46. Ashton I, Miller A, Bowman W, Suding K. Niche complementarity due to plasticity in resource use: plant partitioning of chemical N forms. Ecology. 2010;91:3252-60 pubmed
    ..The existence of plasticity in resource use among the dominant species provides a mechanism that helps to explain the manner by which plant species with broadly overlapping resource use might coexist...
  47. Jaeger A, Lecomte V, Weimerskirch H, Richard P, Cherel Y. Seabird satellite tracking validates the use of latitudinal isoscapes to depict predators' foraging areas in the Southern Ocean. Rapid Commun Mass Spectrom. 2010;24:3456-60 pubmed publisher
    ..The combination of device deployments with sampling of relevant tissues for isotopic analysis appears to be a powerful tool for investigating consumers' isoscapes at various spatio-temporal scales...
  48. Cardona L, Alvarez de Quevedo I, Borrell A, Aguilar A. Massive consumption of gelatinous plankton by Mediterranean apex predators. PLoS ONE. 2012;7:e31329 pubmed publisher
  49. Szpak P, Longstaffe F, Millaire J, White C. Stable isotope biogeochemistry of seabird guano fertilization: results from growth chamber studies with maize (Zea mays). PLoS ONE. 2012;7:e33741 pubmed publisher
    ..Furthermore, the fertilization of maize with seabird guano creates an isotopic signature very similar to a high-trophic level marine resource, which must be considered when interpreting isotopic data from archaeological material. ..
  50. Seminoff J, Benson S, Arthur K, Eguchi T, Dutton P, Tapilatu R, et al. Stable isotope tracking of endangered sea turtles: validation with satellite telemetry and δ15N analysis of amino acids. PLoS ONE. 2012;7:e37403 pubmed publisher
  51. Hartman G, Danin A. Isotopic values of plants in relation to water availability in the Eastern Mediterranean region. Oecologia. 2010;162:837-52 pubmed publisher
    ..From these results it can be concluded that water availability is the primary factor controlling C and N isotope variability in plant communities in the Eastern Mediterranean...
  52. Sherwood O, Lehmann M, Schubert C, Scott D, McCarthy M. Nutrient regime shift in the western North Atlantic indicated by compound-specific ?15N of deep-sea gorgonian corals. Proc Natl Acad Sci U S A. 2011;108:1011-5 pubmed publisher
    ..This evidence suggests that nutrient variability in this region is coordinated with recent changes in global climate and underscores the broad potential of ?(15)N-AA for paleoceanographic studies of the marine N cycle...
  53. Reimchen T, Fox C. Fine-scale spatiotemporal influences of salmon on growth and nitrogen signatures of Sitka spruce tree rings. BMC Ecol. 2013;13:38 pubmed publisher