deuterium

Summary

Summary: The stable isotope of hydrogen. It has one neutron and one proton in the nucleus.

Top Publications

  1. Glöggler S, Müller R, Colell J, Emondts M, Dabrowski M, Blümich B, et al. Para-hydrogen induced polarization of amino acids, peptides and deuterium-hydrogen gas. Phys Chem Chem Phys. 2011;13:13759-64 pubmed publisher
    ..on the chemical state of the used catalyst and the investigated amino acid or peptide, hyperpolarized hydrogen-deuterium gas is formed, which was detected with (1)H and (2)H NMR spectroscopy at low magnetic fields of B(0) = 3...
  2. Sitkiewicz E, Tarnowski K, Poznański J, Kulma M, Dadlez M. Oligomerization interface of RAGE receptor revealed by MS-monitored hydrogen deuterium exchange. PLoS ONE. 2013;8:e76353 pubmed publisher
    ..In this study, we used hydrogen-deuterium exchange and mass spectrometry to map structural differences between the monomeric and oligomeric forms of RAGE...
  3. Misran O, Timimi B, Heidelberg T, Sugimura A, Hashim R. Deuterium NMR Investigation of the lyotropic phases of alkyl ?-glycoside/D2O systems. J Phys Chem B. 2013;117:7335-44 pubmed publisher
    ..of four glycosides (?C8OGlc, ?C8SGlc, ?C10OGlc, ?C8OGal) in water and D2O by optical polarizing microscopy and deuterium NMR...
  4. Hansen P, Kamounah F, Gryko D. Deuterium isotope effects on ¹³C-NMR chemical shifts of 10-hydroxybenzo[h]quinolines. Molecules. 2013;18:4544-60 pubmed publisher
    b>Deuterium isotope effects on ¹³C-NMR chemical shifts are investigated in a series of 10-hydroxybenzo[h]quinolines (HBQ's) The OH proton is deuteriated...
  5. Hammel S, East K, Shaka A, Rose M, Shahrestani P. Brief early-life non-specific incorporation of deuterium extends mean life span in Drosophila melanogaster without affecting fecundity. Rejuvenation Res. 2013;16:98-104 pubmed publisher
    ..Up to 22.5% deuterium oxide (D?O) in H?O was administered, with the result that a low percentage of D?O in the water increased mean ..
  6. Nakazawa S, Ahn J, Hashii N, Hirose K, Kawasaki N. Analysis of the local dynamics of human insulin and a rapid-acting insulin analog by hydrogen/deuterium exchange mass spectrometry. Biochim Biophys Acta. 2013;1834:1210-4 pubmed publisher
    ..In our previous study performed using hydrogen/deuterium exchange mass spectrometry (HDX/MS), differences were observed in the rates and levels of deuteration among ..
  7. Maltsev A, Ying J, Bax A. Deuterium isotope shifts for backbone ¹H, ¹?N and ¹³C nuclei in intrinsically disordered protein ?-synuclein. J Biomol NMR. 2012;54:181-91 pubmed
    ..spectral resolution, a feature particularly important for the poorly dispersed IDP spectra, the impact of deuterium isotope shifts on random coil values has not yet been fully characterized...
  8. Trelle M, Hirschberg D, Jansson A, Ploug M, Roepstorff P, Andreasen P, et al. Hydrogen/deuterium exchange mass spectrometry reveals specific changes in the local flexibility of plasminogen activator inhibitor 1 upon binding to the somatomedin B domain of vitronectin. Biochemistry. 2012;51:8256-66 pubmed publisher
    ..We have used hydrogen/deuterium exchange mass spectrometry to assess the inherent structural flexibility of PAI-1 and to monitor the changes ..
  9. Yoshimoto F, Zhou Y, Peng H, Stidd D, Yoshimoto J, Sharma K, et al. Minor activities and transition state properties of the human steroid hydroxylases cytochromes P450c17 and P450c21, from reactions observed with deuterium-labeled substrates. Biochemistry. 2012;51:7064-77 pubmed
    ..We probed their hydrogen atom abstraction mechanisms and regiochemical plasticity with deuterium-labeled substrates: 17-[(2)H]-pregnenolone; 17-[(2)H]-, 16?-[(2)H]-, 21,21,21-[(2)H(3)]-, and 21-[(2)H]-..

Scientific Experts

More Information

Publications62

  1. Zhang Z, Zhang A, Xiao G. Improved protein hydrogen/deuterium exchange mass spectrometry platform with fully automated data processing. Anal Chem. 2012;84:4942-9 pubmed publisher
    Protein hydrogen/deuterium exchange (HDX) followed by protease digestion and mass spectrometric (MS) analysis is accepted as a standard method for studying protein conformation and conformational dynamics...
  2. Guttman M, Weis D, Engen J, Lee K. Analysis of overlapped and noisy hydrogen/deuterium exchange mass spectra. J Am Soc Mass Spectrom. 2013;24:1906-12 pubmed publisher
    Noisy and overlapped mass spectrometry data hinder the sequence coverage that can be obtained from hydrogen deuterium exchange analysis, and places a limit on the complexity of the samples that can be studied by this technique...
  3. Zhang Y, Rempel D, Zhang J, Sharma A, Mirica L, Gross M. Pulsed hydrogen-deuterium exchange mass spectrometry probes conformational changes in amyloid beta (A?) peptide aggregation. Proc Natl Acad Sci U S A. 2013;110:14604-9 pubmed publisher
    ..The approach incorporates pulsed hydrogen-deuterium exchange coupled with MS analysis...
  4. Wei X, Shi X, Zhong W, Zhao Y, Tang Y, Sun W, et al. Chronic alcohol exposure disturbs lipid homeostasis at the adipose tissue-liver axis in mice: analysis of triacylglycerols using high-resolution mass spectrometry in combination with in vivo metabolite deuterium labeling. PLoS ONE. 2013;8:e55382 pubmed publisher
    A method of employing high-resolution mass spectrometry in combination with in vivo metabolite deuterium labeling was developed in this study to investigate the effects of alcohol exposure on lipid homeostasis at the white adipose tissue ..
  5. Lu H, Gu M, Huang Q, Huang J, Lu W, Lu H, et al. Hydrogen/deuterium exchange mass spectrometry and site-directed disulfide cross-linking suggest an important dynamic interface between the two lysostaphin domains. Antimicrob Agents Chemother. 2013;57:1872-81 pubmed publisher
    ..We used hydrogen/deuterium exchange mass spectrometry (H/DX-MS) and site-directed disulfide cross-linking to probe the interface between ..
  6. Cao J, Burke J, Dennis E. Using hydrogen/deuterium exchange mass spectrometry to define the specific interactions of the phospholipase A2 superfamily with lipid substrates, inhibitors, and membranes. J Biol Chem. 2013;288:1806-13 pubmed publisher
    ..molecules, and inhibitors on a molecular basis has advanced in recent years due to the introduction of hydrogen/deuterium exchange mass spectrometry...
  7. Krawczyk B, Ensle P, Müller W, Sussmuth R. Deuterium labeled peptides give insights into the directionality of class III lantibiotic synthetase LabKC. J Am Chem Soc. 2012;134:9922-5 pubmed publisher
    ..We present a deuterium-label based enzyme assay, utilizing a series of peptide substrates with ?-deuterated Ser, for the determination ..
  8. Elia M, Betts P, Jackson D, Mulligan J. Fetal programming of body dimensions and percentage body fat measured in prepubertal children with a 4-component model of body composition, dual-energy X-ray absorptiometry, deuterium dilution, densitometry, and skinfold thicknesses. Am J Clin Nutr. 2007;86:618-24 pubmed
    ..The effects on and consequences of body build (dimensions, mass, and length of body segments) are unclear...
  9. Lundström P, Hansen D, Vallurupalli P, Kay L. Accurate measurement of alpha proton chemical shifts of excited protein states by relaxation dispersion NMR spectroscopy. J Am Chem Soc. 2009;131:1915-26 pubmed publisher
    ..Thus, the methodology presented is a valuable addition to a growing list of experiments for characterizing excited protein states that are difficult to study using the traditional techniques of structural biology...
  10. Abad J, Camps F, Fabrias G. Substrate-dependent stereochemical course of the (Z)-13-desaturation catalyzed by the processionary moth multifunctional desaturase. J Am Chem Soc. 2007;129:15007-12 pubmed
    ..In the synthetic pathway, a functionalized acetylene common synthon was used for introducing the four deuterium tags...
  11. O Halloran S, Ilett K. Evaluation of a deuterium-labeled internal standard for the measurement of sirolimus by high-throughput HPLC electrospray ionization tandem mass spectrometry. Clin Chem. 2008;54:1386-9 pubmed publisher
    ..A stable isotope-labeled IS can be used to minimize analytical interpatient variation...
  12. Krempels K, Somlyai I, Somlyai G. A retrospective evaluation of the effects of deuterium depleted water consumption on 4 patients with brain metastases from lung cancer. Integr Cancer Ther. 2008;7:172-81 pubmed publisher
    ..A new concept based on the different chemical and physical behavior of protium and deuterium affecting cell signaling and tumor growth has been introduced in the treatment of cancer patients...
  13. Zhang Q, Willison L, Tripathi P, Sathe S, Roux K, Emmett M, et al. Epitope mapping of a 95 kDa antigen in complex with antibody by solution-phase amide backbone hydrogen/deuterium exchange monitored by Fourier transform ion cyclotron resonance mass spectrometry. Anal Chem. 2011;83:7129-36 pubmed publisher
  14. Ma L, Kaufman Y, Zhang J, Washington I. C20-D3-vitamin A slows lipofuscin accumulation and electrophysiological retinal degeneration in a mouse model of Stargardt disease. J Biol Chem. 2011;286:7966-74 pubmed publisher
    ..In this work we investigated the effects of long-term administration of deuterium-enriched vitamin A, C20-D(3)-vitamin A, on RPE lipofuscin deposition and eye function in a mouse model of ..
  15. Carulla N, Zhou M, Giralt E, Robinson C, Dobson C. Structure and intermolecular dynamics of aggregates populated during amyloid fibril formation studied by hydrogen/deuterium exchange. Acc Chem Res. 2010;43:1072-9 pubmed publisher
    ..Hydrogen-deuterium (HD) exchange experiments are based on solvent accessibilities and provide one means by which this kind of ..
  16. Bexelius C, Lof M, Sandin S, Trolle Lagerros Y, Forsum E, Litton J. Measures of physical activity using cell phones: validation using criterion methods. J Med Internet Res. 2010;12:e2 pubmed publisher
    ..Data collected on physical activity in large epidemiological studies is often based on paper questionnaires. The validity of these questionnaires is debated, and more effective methods are needed...
  17. Stoychev S, Nathaniel C, Fanucchi S, Brock M, Li S, Asmus K, et al. Structural dynamics of soluble chloride intracellular channel protein CLIC1 examined by amide hydrogen-deuterium exchange mass spectrometry. Biochemistry. 2009;48:8413-21 pubmed publisher
    ..5 in the absence of membranes by amide hydrogen-deuterium exchange mass spectrometry (DXMS)...
  18. Smirnovas V, Baron G, Offerdahl D, Raymond G, Caughey B, Surewicz W. Structural organization of brain-derived mammalian prions examined by hydrogen-deuterium exchange. Nat Struct Mol Biol. 2011;18:504-6 pubmed publisher
    ..Here we used mass spectrometry analysis of hydrogen-deuterium exchange to examine brain-derived PrP(Sc)...
  19. Kaufman Y, Ma L, Washington I. Deuterium enrichment of vitamin A at the C20 position slows the formation of detrimental vitamin A dimers in wild-type rodents. J Biol Chem. 2011;286:7958-65 pubmed publisher
    ..We prepared vitamin A enriched with the stable isotope deuterium at carbon twenty (C20-D(3)-vitamin A)...
  20. Rudowska M, Wojewska D, Kluczyk A, Bąchor R, Stefanowicz P, Szewczuk Z. The hydrogen-deuterium exchange at ?-carbon atom in N,N,N-trialkylglycine residue: ESI-MS studies. J Am Soc Mass Spectrom. 2012;23:1024-8 pubmed publisher
    ..Increased ionization efficiency, provided by the fixed positive charge on QAS group, as well as the deuterium labeling, enables the analysis of trace amounts of peptides.
  21. Meilleur F, Weiss K, Myles D. Deuterium labeling for neutron structure-function-dynamics analysis. Methods Mol Biol. 2009;544:281-92 pubmed publisher
    ..The particular sensitivity of neutrons to the isotopes of hydrogen makes selective deuterium labeling of biological systems an essential tool for maximizing the return from neutron scattering experiments...
  22. Zhang J, Chalmers M, Stayrook K, Burris L, Garcia Ordonez R, Pascal B, et al. Hydrogen/deuterium exchange reveals distinct agonist/partial agonist receptor dynamics within vitamin D receptor/retinoid X receptor heterodimer. Structure. 2010;18:1332-41 pubmed publisher
    ..Previously, we demonstrated that hydrogen/deuterium exchange (HDX) can be applied to determine novel mechanism of action of PPAR? ligands and in predicting tissue ..
  23. Marques M, Vaso A, Neto J, Fossey M, Oliveira J, Basso L, et al. Dynamics of glyphosate-induced conformational changes of Mycobacterium tuberculosis 5-enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) determined by hydrogen-deuterium exchange and electrospray mass spectrometry. Biochemistry. 2008;47:7509-22 pubmed publisher
    ..This may be part of the mechanism of inhibition of the enzyme, which is required to prevent the hydration of the substrate binding site and also to induce the cleft closure to avoid entrance of the substrates...
  24. Eichhorn G, Visser G. Evaluation of the deuterium dilution method to estimate body composition in the barnacle goose: accuracy and minimum equilibration time. Physiol Biochem Zool. 2008;81:508-18 pubmed publisher
    We examined body composition in barnacle geese (Branta leucopsis) by proximate carcass analysis and by deuterium isotope dilution...
  25. Hamuro Y, Molnar K, Coales S, Ouyang B, Simorellis A, Pochapsky T. Hydrogen-deuterium exchange mass spectrometry for investigation of backbone dynamics of oxidized and reduced cytochrome P450cam. J Inorg Biochem. 2008;102:364-70 pubmed
    ..P450(cam) (CYP101) as a function of oxidation/ligation state of the heme iron were investigated via hydrogen/deuterium exchange (H/D exchange) as monitored by mass spectrometry...
  26. Ayala I, Sounier R, Usé N, Gans P, Boisbouvier J. An efficient protocol for the complete incorporation of methyl-protonated alanine in perdeuterated protein. J Biomol NMR. 2009;43:111-9 pubmed publisher
    ..Our cost effective strategy offers a significant enhancement in the level of incorporation of methyl-labeled alanine in overexpressed proteins over previously reported methods...
  27. Daily A, Greathouse D, van der Wel P, Koeppe R. Helical distortion in tryptophan- and lysine-anchored membrane-spanning alpha-helices as a function of hydrophobic mismatch: a solid-state deuterium NMR investigation using the geometric analysis of labeled alanines method. Biophys J. 2008;94:480-91 pubmed
    We used solid-state deuterium NMR spectroscopy and geometric analysis of labeled alanines to investigate the structure and orientation of a designed synthetic hydrophobic, membrane-spanning alpha-helical peptide that is anchored within ..
  28. Manalo M, Perez L, LiWang A. Hydrogen-bonding and pi-pi base-stacking interactions are coupled in DNA, as suggested by calculated and experimental trans-Hbond deuterium isotope shifts. J Am Chem Soc. 2007;129:11298-9 pubmed
  29. Lu Y, Qu F, Moore B, Endicott D, Kuester W. Hydride reduction of NAD+ analogues by isopropyl alcohol: kinetics, deuterium isotope effects and mechanism. J Org Chem. 2008;73:4763-70 pubmed publisher
    ..The corresponding deuterium kinetic isotope effects (e.g...
  30. Farmer A, Cade B, Torres Dowdall J. Fundamental limits to the accuracy of deuterium isotopes for identifying the spatial origin of migratory animals. Oecologia. 2008;158:183-92 pubmed publisher
    b>Deuterium isotope analyses have revolutionized the study of migratory connectivity because global gradients of deuterium in precipitation (deltaD(P)) are expressed on a continental scale...
  31. Rand K, Pringle S, Morris M, Brown J. Site-specific analysis of gas-phase hydrogen/deuterium exchange of peptides and proteins by electron transfer dissociation. Anal Chem. 2012;84:1931-40 pubmed publisher
    To interpret the wealth of information contained in the hydrogen/deuterium exchange (HDX) behavior of peptides and proteins in the gas-phase, analytical tools are needed to resolve the HDX of individual exchanging sites...
  32. Liu A, Byrne N, Ma G, Nasreddine L, Trinidad T, Kijboonchoo K, et al. Validation of bioelectrical impedance analysis for total body water assessment against the deuterium dilution technique in Asian children. Eur J Clin Nutr. 2011;65:1321-7 pubmed publisher
    ..To develop and cross-validate bioelectrical impedance analysis (BIA) prediction equations of total body water (TBW) and fat-free mass (FFM) for Asian pre-pubertal children from China, Lebanon, Malaysia, Philippines and Thailand...
  33. Macallan D, Asquith B, Zhang Y, de Lara C, Ghattas H, Defoiche J, et al. Measurement of proliferation and disappearance of rapid turnover cell populations in human studies using deuterium-labeled glucose. Nat Protoc. 2009;4:1313-27 pubmed publisher
    ..b>Deuterium-labeled glucose is one such precursor which, because it achieves high levels of enrichment for a short period, ..
  34. Figueira A, Saidemberg D, Souza P, Martinez L, Scanlan T, Baxter J, et al. Analysis of agonist and antagonist effects on thyroid hormone receptor conformation by hydrogen/deuterium exchange. Mol Endocrinol. 2011;25:15-31 pubmed publisher
    ..In this study, we conducted hydrogen/deuterium exchange on TR LBDs with or without agonist (T(3)) or antagonist (NH3)...
  35. Stokasimov E, Rubenstein P. Actin isoform-specific conformational differences observed with hydrogen/deuterium exchange and mass spectrometry. J Biol Chem. 2009;284:25421-30 pubmed publisher
    ..We used amide proton hydrogen/deuterium (HD) exchange detected by mass spectrometry to analyze conformational differences between Saccharomyces ..
  36. Kupniewska Kozak A, Gospodarska E, Dadlez M. Intertwined structured and unstructured regions of exRAGE identified by monitoring hydrogen-deuterium exchange. J Mol Biol. 2010;403:52-65 pubmed publisher
    ..In this work, we used hydrogen-deuterium exchange and mass spectrometry to gain insight into the structural properties of exRAGE (extracellular region of ..
  37. Ganusov V, Borghans J, de Boer R. Explicit kinetic heterogeneity: mathematical models for interpretation of deuterium labeling of heterogeneous cell populations. PLoS Comput Biol. 2010;6:e1000666 pubmed publisher
    ..b>Deuterium, in the form of deuterated glucose or heavy water, can be used to measure rates of proliferation and death of ..
  38. Quiterio A, Silva A, Minderico C, Carnero E, Fields D, Sardinha L. Total body water measurements in adolescent athletes: a comparison of six field methods with deuterium dilution. J Strength Cond Res. 2009;23:1225-37 pubmed publisher
    ..e., deuterium dilution) in 118 adolescent athletes...
  39. Religa T, Kay L. Optimal methyl labeling for studies of supra-molecular systems. J Biomol NMR. 2010;47:163-9 pubmed publisher
    ..Thus, labeling of supra-molecules with (13)CH(3) isotopomers remains the method of choice, but in applications where (13)CHD(2) moieties are required, sensitivity will in general not be limiting...
  40. Zhang X, Chien E, Chalmers M, Pascal B, Gatchalian J, Stevens R, et al. Dynamics of the beta2-adrenergic G-protein coupled receptor revealed by hydrogen-deuterium exchange. Anal Chem. 2010;82:1100-8 pubmed publisher
    ..human beta(2) adrenergic receptor bound to the inverse agonist carazolol using the technique of amide hydrogen/deuterium exchange coupled with mass spectrometry (HDX MS)...
  41. Lalli D, Schanda P, Chowdhury A, Retel J, Hiller M, Higman V, et al. Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins. J Biomol NMR. 2011;51:477-85 pubmed publisher
    ..Sufficient signal-to-noise was achieved due to the short deuterium T (1), allowing for high repetition rates and enabling 3D experiments with a (2)H-(13)C ..
  42. Full S, Deinzer M, Ho P, Greenwood J. Phosphoinositide binding regulates alpha-actinin CH2 domain structure: analysis by hydrogen/deuterium exchange mass spectrometry. Protein Sci. 2007;16:2597-604 pubmed
    ..by which phosphoinositide binding regulates alpha-actinin function using mass spectrometry to monitor hydrogen-deuterium (H/D) exchange within the calponin homology 2 domain...
  43. Hsu Y, Johnson D, Traugh J. Analysis of conformational changes during activation of protein kinase Pak2 by amide hydrogen/deuterium exchange. J Biol Chem. 2008;283:36397-405 pubmed publisher
    ..Changes at each step were individually analyzed by amide hydrogen/deuterium exchange coupled with mass spectrometry and compared with inactive Pak2...
  44. Salomonsson L, Brändén G, Brzezinski P. Deuterium isotope effect of proton pumping in cytochrome c oxidase. Biochim Biophys Acta. 2008;1777:343-50 pubmed publisher
    ..mechanism of electron-proton coupling in cytochrome c oxidase we have studied the pH-dependence of the kinetic deuterium isotope effect of specific reaction steps associated with proton transfer in wild-type and structural variants ..
  45. Liao W, Dodder N, Mast N, Pikuleva I, Turko I. Steroid and protein ligand binding to cytochrome P450 46A1 as assessed by hydrogen-deuterium exchange and mass spectrometry. Biochemistry. 2009;48:4150-8 pubmed publisher
    ..This P450 can interact with different steroid substrates and protein redox partners. We utilized hydrogen-deuterium (H-D) exchange mass spectrometry for investigating CYP46A1-ligand interactions...
  46. Chesson L, Valenzuela L, O Grady S, Cerling T, Ehleringer J. Hydrogen and oxygen stable isotope ratios of milk in the United States. J Agric Food Chem. 2010;58:2358-63 pubmed publisher
    ..As an application example, milk water delta(18)O values were used to predict possible regions of origin for restaurant samples...
  47. Baldwin A, Religa T, Hansen D, Bouvignies G, Kay L. 13CHD2 methyl group probes of millisecond time scale exchange in proteins by 1H relaxation dispersion: an application to proteasome gating residue dynamics. J Am Chem Soc. 2010;132:10992-5 pubmed publisher
    ..The methodology is applied to the gating residues of the T. acidiphilium proteasome that are shown to undergo extensive motion on the millisecond time scale...
  48. Soulard P, McLaughlin M, Stevens J, Connolly B, Coli R, Wang L, et al. Development of a high-throughput screening assay for stearoyl-CoA desaturase using rat liver microsomes, deuterium labeled stearoyl-CoA and mass spectrometry. Anal Chim Acta. 2008;627:105-11 pubmed publisher
    ..inhibitors of SCD1, we have developed a mass spectrometry based high-throughput screening (HTS) assay using deuterium labeled stearoyl-CoA substrate and induced rat liver microsomes...
  49. Ruschak A, Velyvis A, Kay L. A simple strategy for ¹³C, ¹H labeling at the Ile-?2 methyl position in highly deuterated proteins. J Biomol NMR. 2010;48:129-35 pubmed publisher
    ..This labeling scheme adds to a growing list of different approaches that are significantly impacting the utility of solution NMR spectroscopy in studies of supra-molecular systems...
  50. Lu S, Li W, Rotem D, Mikhailova E, Bayley H. A primary hydrogen-deuterium isotope effect observed at the single-molecule level. Nat Chem. 2010;2:921-8 pubmed publisher
    ..Remarkably, a primary hydrogen-deuterium isotope effect is readily detected at the single-molecule level during prototropic rearrangement of an initial ..
  51. Ducho C, Hamed R, Batchelar E, Sorensen J, Odell B, Schofield C. Synthesis of regio- and stereoselectively deuterium-labelled derivatives of L-glutamate semialdehyde for studies on carbapenem biosynthesis. Org Biomol Chem. 2009;7:2770-9 pubmed publisher
    ..Regioselective deuterium incorporation into the 5-position of L-GSA was achieved using a labelled form of the Schwartz reagent (Cp2Zr2HCl)..
  52. Olofsson A, Lindhagen Persson M, Vestling M, Sauer Eriksson A, Ohman A. Quenched hydrogen/deuterium exchange NMR characterization of amyloid-beta peptide aggregates formed in the presence of Cu2+ or Zn2+. FEBS J. 2009;276:4051-60 pubmed publisher
    ..of Abeta(1-40) and Abeta(1-42) in the presence or absence of either Cu(2+) or Zn(2+) using quenched hydrogen/deuterium exchange combined with solution NMR spectroscopy...
  53. Thielges M, Case D, Romesberg F. Carbon-deuterium bonds as probes of dihydrofolate reductase. J Am Chem Soc. 2008;130:6597-603 pubmed publisher
    ..We have been developing the use of carbon-deuterium bonds as probes of proteins and now report the application of the technique to the enzyme dihydrofolate ..